JP7376899B2 - Skin external preparations - Google Patents

Description

The present invention relates to a novel active ingredient derived from a natural product and having excellent physiological activity and biosafety, and an external preparation for the skin (including the scalp) and an oral composition for beauty or health promotion, which are formulated with the same. .

In recent years, research has been conducted on cell aging and damage to cells caused by external factors (e.g., ultraviolet rays, chemicals such as air pollutants and environmental hormones, allergens such as pollen, environmental stress, etc.), and various cell Mechanisms related to aging phenomena, cell damage, prevention, and recovery have been elucidated.

Active ingredients for preventing and improving the above-mentioned cell damage and aging phenomena have been proposed, and cosmetics, food and drink products, and pharmaceuticals containing these active ingredients have been put on the market. For example, antioxidants such as vitamin C, vitamin E, catalase, or their derivatives; anti-inflammatory agents such as glycyrrhizic acid or its salts, allantoin, tranexamic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract and γ-amino-β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin, hyaluronic acid or its salts; humectants such as urea; and protein glycation inhibitors such as aminoguanidine.

However, these conventional components have problems in terms of stability, safety, and effectiveness when actually applied to living organisms. For example, the above-mentioned ascorbic acid or its derivatives are easily oxidized by external factors such as ultraviolet rays or enzymes in the body, and there is a problem that their uptake into cells decreases with age.

As a result of intensive research to solve the above problems, the present inventors have discovered a fermented product of a plant of the Nelumbo family (Nelumbonaceae), a hydrolyzate of a plant of the genus Oryza (Poaceae), Extracts of Salicornia plants of the Chenopodiaceae family, and hydrolysates of soybeans or their extracts have been found to be effective in producing vitamin C transporters (vitamin C transporters), which are vitamin C channels involved in the uptake of vitamin C into cells. It was also found that it has the effect of promoting the synthesis of SVCT).

Conventionally, inventions have been made in which fermented products of plants of the genus Lotus of the Lotus family, hydrolysates of plants of the genus Poaceae of the genus Poaceae, extracts of plants of the genus Salicorum of the family Chenopodiaceae, and hydrolysates of soybeans or their extracts are used in external preparations for the skin. was known, for example, from Patent Documents 1 to 5. However, fermented products of plants of the Lotus genus of the Lotus family, extracts or hydrolysates of plants of the genus Poaceae of the Poaceae family, extracts of plants of the genus Salicorum of the Chenopodiaceae family, and hydrolysates of soybeans or their extracts are used to synthesize SVCT1. It was not known to have a promoting effect.
JP2005-298489 JP 2002-128632 JP2013-103906 JP 2005-145878 JP 2006-290742

The present invention provides vitamin C containing as an active ingredient a fermented product of a plant of the genus Lotus of the lotus family, a hydrolyzate of a genus of the genus Poaceae of the genus Poaceae, an extract of a plant of the genus Salicorum of the family Chenopodiaceae, or a hydrolyzate of soybean or an extract thereof. It is an uptake promoter.
The present invention is an external skin preparation containing the vitamin C uptake promoter described above.
The present invention is an oral composition containing the above vitamin C uptake promoter.

The extract, hydrolyzate, or fermented product according to the present invention can enhance the expression of SVCT-1, which is the uptake site for vitamin C in pigment cells, and therefore can be used as an active ingredient for whitening or antioxidant purposes. By using it in combination with vitamin C, it is possible to increase its uptake into pigment cells and obtain a more effective whitening effect.

The present invention will be explained in detail below.
Examples of plants of the Nelumbo family (Nelumbonaceae) used in the present invention include Nelumbo nucifera and Nelumbo Lutea.

When obtaining a fermented product by microorganisms (yeast, lactic acid bacteria, Aspergillus or Bacillus subtilis) using plants of the genus Lotus, there are no particular restrictions on the fermentation parts of the plant, including whole plants, leaves, flowers, stamens, pistils, and stems. Although appropriate parts such as , rhizomes, and seeds (grains) can be used, it is preferable to use the whole plant or seeds from the viewpoint of the effectiveness of the fermented product obtained.

In addition, as the plants of the Poaceae family (Poaceae) and the Oryza genus (Oryza) used in the present invention, any plant that belongs to Oryza sativa taxonomically can be used, and specifically, for example, Koshihikari, Sasanishiki, Examples include Nihonbare, Akita Makachi, Kinuhikari, Hana Echizen, etc., but are of course not limited to these. In the present invention, there are no particular limitations on the part of the plant belonging to the genus Poaceae, and any suitable parts such as the whole plant, leaves, flowers, stems, roots, and seeds (grains) can be used. preferable. Moreover, when using leaves, it is more preferable to use leaves before heading. In addition, when using rice leaves, the leaves may be subjected to heat treatment before the extraction treatment. Thereby, it is possible to suppress denaturation and deterioration due to active ingredients (such as enzymes) contained in the rice leaves, and it is possible to prevent a decrease in activity of the collected rice leaves during storage.

Examples of plants of the genus Salicornia in the Chenopodiaceae family include Salicornia europaea, Salicornia bigelovii, Salicornia dolichostachia S. sp. strictisima, but the present invention is not limited thereto. do not have. Parts of these plants used include whole plants, flowers, leaves, stems, seeds, and the like.

The soybean used as a material in the present invention is a plant that is classified as the same species as soybean (scientific name: Glycine max (L.) Merrill) in botanical taxonomy. As soybeans, any of white soybeans (yellow soybeans), blue soybeans, black soybeans, etc. can be used.

When preparing an extract from the above plants, it can be done, for example, as follows. That is, first, if necessary, the extracted part of each plant is washed with water in advance to remove foreign substances, and then it is left as is or dried, and if necessary, it is chopped or crushed, and brought into contact with an extraction solvent to perform extraction. Extraction can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method, but it is also possible to use a supercritical extraction method or a steam distillation method.

Extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; ethylene glycol, 1,3-propanediol, 1,3-butylene glycol, Polyhydric alcohols such as glycerin; Esters such as ethyl acetate, butyl acetate, methyl propionate, and glyceryl trioctanoate; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether, isopropyl, and ether; n-hexane, toluene , hydrocarbon solvents such as chloroform, and the like, which can be used alone or in combination of two or more.

When using a mixed solvent, the mixing ratio is, for example, 1:1 to 25:1 by volume (the same applies hereinafter) for a mixed solvent of water and ethyl alcohol, and 1:1 for a mixed solvent of water and glycerin. In the case of a mixed solvent of water and 1,3-propanediol or 1,3-butylene glycol, the ratio is preferably in the range of 1:1 to 15:1.

There are no particular limitations on the pH when preparing the extract, but it is generally preferred to keep it in the range of 3 to 9. In this sense, if necessary, an alkaline regulator such as sodium hydroxide, sodium carbonate, potassium hydroxide, or an acidic regulator such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be added to the extraction solvent to obtain the desired effect. The pH may be adjusted to .

Extraction conditions such as extraction temperature and extraction time vary depending on the type of solvent used and pH, but for example, when using water, 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol as the solvent. If so, the extraction temperature is preferably in the range of 0°C to 90°C, and the extraction time is preferably 0.5 hours to 7 days.

Here, prior to the extraction treatment of the present invention or in parallel with the extraction treatment, the extraction site may be subjected to a hydrolysis treatment as necessary. This may improve the skin irritation, effectiveness, storage stability, etc. of the extract and make it possible to use the extract more effectively.

In particular, in the present invention, it is preferable to hydrolyze extracts of rice leaves and soybeans. Examples of hydrolysis treatment methods include decomposition treatment methods using acids, alkalis, or enzymes.

When performing hydrolysis treatment using an enzyme, at least one enzyme selected from proteolytic enzymes, starch-degrading enzymes, pectin-degrading enzymes, fibrinolytic enzymes, and lipolytic enzymes can be used. .

Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, and peptidases such as glycylglycine peptidase, carboxypeptidase, and aminopeptidase. , bromelain, a complex proteolytic enzyme derived from a microorganism (for example, Neurase [manufactured by Amano Enzyme Co., Ltd.]), and the like can be used.

As the starch degrading enzyme, for example, α-amylase, β-amylase, glucoamylase, β-galactosidase, etc. can be used.

As the pectin degrading enzyme, for example, pectinase, pectin depolymerase, pectin demethoxylase, pectin lyase, pectin esterase, polygalacturonase, etc. can be used.

As the fibrinolytic enzyme, for example, cellulase, hemicellulase, agarase, mannase, chitinase, chitosanase, carrageenase, arginase, fucoidanase, inulase, xylanase, ligninase, etc. can be used.

As the lipolytic enzyme, for example, lipase, phospholipase, etc. can be used.

Further, in the present invention, fermentation treatment may be performed using microorganisms (lactic acid bacteria, yeast, Aspergillus or Bacillus subtilis, etc.) for the purpose of improving the effectiveness, suppressing skin irritation, or improving stability of the extracted material. In particular, in the present invention, it is preferable that plants of the genus Lotus or extracts thereof are subjected to fermentation treatment using microorganisms.

When fermentation is carried out, it can be carried out, for example, as follows. First, as a resource for fermentation, the plant itself (hereinafter sometimes referred to as a plant body) may be used, or an extract obtained by extracting the plant body with an appropriate medium may be used. Furthermore, when using an extract, it is also possible to perform fermentation while containing the plant body, without removing the plant body to be extracted by solid-liquid separation. Here, the plant may be fresh or may be dried or semi-dried in advance. Moreover, it is also possible to use the sampled shape as it is.

In the present invention, lactic acid bacteria include, for example, lactic acid bacteria of the genus Lactobacillus, such as Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus delbrueckii; Lactic acid bacteria of the genus Carnobacterium such as Carnobacterium divergens and Carnobacterium piscicola; Leuconostoc mesenteroides, Leuconostoc lactis, Leuco Lactic acid bacteria of the genus Leuconostoc such as Leuconostoc citreum; Lactic acid bacteria of the genus Streptococcus such as Streptococcus faecalis and Streptococcus pyogenes; Enterococcus caseliflavus, Enterococcus Sulphureus (Enterococcus Lactic acid bacteria of the genus Enterococcus, such as Lactococcus sulfreus; Lactococcus plantarum; Lactic acid bacteria of the genus Lactococcus, such as Lactococcus raffinolactis; Weissella confusa, Weissella kandleri, etc. Lactic acid bacteria belonging to Vacella; Atopobium Minutum, Atopobium Purvulus (AtopobiumParvulus) lactic acid bacteria (VAGOCO (VAGOCO) Lactic acid bacteria belonging to Bagococcus such as CCUS Fluvialis), Bagococcus Salmoninarum, etc. ; Examples include lactic acid bacteria of the genus Pediococcus such as Pediococcus damnosus and Pediococcus pentosaceus.

In the present invention, yeasts include, for example, Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, Saccharomyces bayonus. ) etc. Yeasts of the genus Saccharomyces, yeasts of the genus Galactomyces, yeasts of the genus Torulaspora such as Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei, Zygosaccharomyces rouxii, Yeasts of the genus Zygosaccharomyces such as Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, Zygosaccharomyces lactis, Candida versatilis ), Candida Echerisii ( Yeasts of the genus Candida such as Candida etchellsii, Candida kefyr, Candida sake, Candida scotii, Aureobasidium pullulans, Aureobasidium mansoni yeasts of the genus Aureobasidium such as Aureobasidium microstictum (Aureobasidium microstictum) and Aureobasidium microstictum (Aureobasidium microstictum). Furthermore, the yeast according to the present invention may be any of sake yeast, wine yeast, beer yeast, yeast derived from plant flowers (roses, lilies, cherry blossoms, etc.), and yeast derived from the sea.

Furthermore, in the present invention, koji molds include, for example, yellow koji molds such as Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus awamori, etc. ), black koji molds such as Aspergillus kawauchii, Aspergillus usami, and Aspergillus niger, and red koji molds such as Monascus anka and Monascus pilosus.

Furthermore, in the present invention, Bacillus subtilis includes, for example, Bacillus natto, Bacillus subtilis, Bacillus circulans, and the like.

When the above-mentioned suspension or extract is fermented by microorganisms, it is necessary to sterilize the suspension or extract before the fermentation process to remove bacteria that may interfere with the fermentation. As a method for sterilizing and removing bacteria, a method may be used in which the fermented material is washed in advance with sterilizing ethanol and then suspended in a sterile solvent such as sterile water, or a method in which the fermented material is suspended in a solvent and then suspended. The suspension may be sterilized by heat sterilization or the like. Heat sterilization treatments include autoclave sterilization in which the suspension is heated at 120 to 130°C for 10 to 20 minutes, and intermittent sterilization in which the suspension is held at 80 to 90°C for 60 to 120 minutes once a day for 2 to 3 days. Heat sterilization methods such as sterilization methods are generally used.

The sterilized suspension is placed in a fermentation tank, where microorganisms are inoculated and fermented. The appropriate amount of microorganisms to be inoculated is 10 ⁷ to 10 ⁸ cells/mL. If the amount of inoculation exceeds the above range, the progress time of fermentation will hardly change, while if it falls below the above range, it will take a long time to complete the fermentation, which is not preferable.

Fermentation temperature is generally in the range of 5 to 50°C, preferably 20 to 40°C, which is the optimum growth temperature for each microorganism (for example, 30 to 40°C for lactic acid bacteria, 25 to 30°C for yeast). is within the range of The number of days for fermentation is generally in the range of 1 to 10 days, preferably 2 to 5 days at the optimum temperature. If the number of fermentation days is shorter than the above general range, fermentation will not occur sufficiently and the effectiveness of the fermented product will tend to decrease, while if it is extended beyond 10 days, no further increase in effectiveness will be observed. In addition, coloring and fermentation odor increase, both of which are undesirable.

When preparing fermented products from plants of the genus Lotus, the suspension or The extract solution may be subjected to the hydrolysis treatment described above.

The extract, hydrolyzate, or fermented product prepared as described above may be used as it is, generally after adjusting the pH to 3 to 9, or it may be concentrated to a desired concentration by vacuum concentration, etc. May be used as Alternatively, it may be dried by a conventional method such as spray drying.

Moreover, in order to improve the storage stability of the extract, hydrolyzate, or fermented product prepared as described above, the supernatant may be used after being refrigerated for a certain period of time.

The extract, hydrolyzate, or fermented product according to the present invention can be incorporated into external skin preparations (cosmetics, quasi-drugs, external medicines), and food and drinks for beauty or health promotion (oral compositions). can. Examples of external skin preparations include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, blushers, whitening powders, face washes, body shampoos, scalp and hair shampoos, and hair conditioners. , shampoos or tonics for hair growth, hair nourishing, cleaning cosmetics such as soaps, bath salts, etc., but the present invention is not limited thereto. Foods and beverages for beauty or health promotion include beauty drinks, nutritional drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks, etc.; various soups (including powdered soups), and dairy products. , jelly, candy, tablet confectionery, gum, and other foods; tablets, liquids, granules, or jelly-like health foods/drinks, etc.; however, the present invention is not limited to these; food and beverages that can be taken orally; Can be added to products, etc.

The amount of the extract, hydrolyzate, or fermented product according to the present invention can be adjusted as appropriate depending on the formulation to be mixed, and the solid content is, for example, 0.002 to 1.0 in the case of basic cosmetics. Weight % (solid content weight %, same hereinafter) range, in the case of makeup cosmetics, the range of 0.002 to 1.0 weight %, and in the case of cleansing cosmetics, the range of 0.002 to 10.0 % by weight. In the case of hair cosmetics, the solid content of the composition is in the range of 0.0001 to 5.0% by weight. Further, the amount incorporated into the oral composition is preferably in the range of 0.1 to 15% by weight in terms of solid content.

In addition to the essential components of the extract, hydrolyzate, or fermented product, the preparation containing the extract, hydrolyzate, or fermented product according to the present invention includes ingredients normally used in external skin preparations and oral compositions. For example, oil-based ingredients, surfactants (synthetic and natural), humectants, thickeners, preservatives and disinfectants, powder ingredients, antioxidants, chelating agents, pH adjusters, pigments, fragrances, etc. are required. It can be blended as appropriate. Furthermore, there is no problem in using it in combination with other physiologically active ingredients as long as the effectiveness and characteristics are not impaired.

Here, examples of oily ingredients include lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cacao oil, meadowfoam oil, Plant-derived fats and oils such as rosehip oil, orchid oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, and plant-derived squalane; Animal-derived fats and oils such as mink oil and turtle oil; beeswax, carnauba wax, and rice Waxes such as wax and lanolin; Hydrocarbons such as liquid paraffin, petrolatum, paraffin wax, and squalane; Fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and eicosenoic acid; lauryl alcohol, cetanol, Higher alcohols such as stearyl alcohol; synthetic esters such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexylglyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.), and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, Nonionic surfactants such as polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates , polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene alkyl phenyl ether phosphate, and other anionic surfactants; quaternary ammonium salts, primary to tertiary fatty amine salts, Cationic surfactants such as trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, polyethylene polyamine fatty acid amide salts; N , N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N',N'-dimethyl-N' -Ampholytic surfactants such as β-hydroxypropylammoniosulfobetaine and the like can be used.

Emulsifiers or emulsification aids include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or its esters, lactose, water-soluble soybean-derived Polysaccharides, complexes of soybean-derived proteins and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc. can also be blended.

Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and saccharides such as trehalose, mucopolysaccharides (e.g. Hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and ) extracts, various amino acids and their derivatives.

Examples of thickeners include components derived from brown algae, green algae, or red algae such as alginic acid, agar, carrageenan, and fucoidan; Silane root extract; pectin, locust bean gum, aloe polysaccharide, alcaligenes-producing polysaccharide, etc. polysaccharides; gums such as xanthan gum, tragacanth gum, and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, and acrylic acid/methacrylic acid copolymer; hyaluronic acid Examples include acids and derivatives thereof; polyglutamic acid and derivatives thereof.

Examples of preservatives and disinfectants include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, and chloride. Benzalkonium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodeinyl urea), polyphosphoric acid, propanediol, 1,2-pentanediol, various essential oils, bark dry distillate, fermented radish liquid, sugarcane, etc. Examples include plant-derived ethanol or 1,3-butylene glycol.

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, There are cellulose powders, grain powders (rice, wheat, corn, millet, etc.), and legume powders (soybeans, adzuki beans, etc.).

Examples of ultraviolet absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy- 4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. be.

Antioxidants include, for example, butylated hydroxyanisole, butylated hydroxytoluene, propyl gallate, extract of violacea, silane root extract, peony extract, vitamin E and its derivatives (e.g., vitamin E nicotinate, vitamin E linoleate). etc.) etc.

Examples of the chelating agent include trisodium ethylenediamine hydroxyethyl triacetate, edetic acid or its salts, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, and tetrasodium hydroxyethane diphosphonate.

Examples of the pH adjuster include citric acid or its salts, lactic acid or its salts, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide, and the like.

Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone and its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives. , 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine mono phosphate, adenosine monophosphate), and these may be blended alone or in combination.

Examples of the above-mentioned kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid sugar derivatives such as kojic acid ethers, and kojic acid glucoside. Examples of ascorbic acid derivatives include sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and sodium L-ascorbic acid 2-phosphate. Ascorbic acid ester salts such as magnesium 2-sulfate ester, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl potassium phosphate, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate ester, L -L-ascorbic acid tetrafatty acid esters such as ascorbic acid tetralaurate, 3-O-ethyl ascorbic acid, sodium L-ascorbic acid-2-phosphate-6-O-palmitate, glyceryl ascorbic acid or its acyl derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and examples of tranexamic acid derivatives include tranexamic acid esters (for example, tranexamic acid lauryl ester). , tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amide derivatives of tranexamic acid (for example, tranexamic acid methylamide), etc., and resorcinol derivatives include, for example, 4-n-butylresorcinol, 4-isoamyl Resorcinol etc. are 2,5-dihydroxybenzoic acid derivatives, such as 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives. Examples of the acid include nicotinamide and benzyl nicotinate, and examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

Physiologically active ingredients include, for example, placenta extract, sagebrush extract, saxifrage extract, perilla extract, rice bran extract or its hydrolyzate, white mustard extract or its hydrolyzate, white mustard fermented product, and peony. Extract or its hydrolyzate, lactic acid bacteria-fermented rice, purple beetroot extract, lotus flower extract, adlay hydrolyzate, adlay seed fermentation product, royal jelly fermentation product, sake lees extract or ceramide contained therein, sake lees fermentation product, Examples include Pandanus amaryllifolius Roxb. extract, Arcangelicia flava Merrilli extract, chamomile extract, and the like. In addition, extracts of eggplants (Lotus, long eggplant, Kamo eggplant, rice eggplant, etc.) or their hydrolysates, apricot fruit extracts, seaweed extracts such as Katamen Kirincho, extracts of marine flowering plants such as eelgrass, Fermented soy milk, jellyfish water, rice extract or its hydrolyzate, fermented rice extract, germinated rice extract or its hydrolyzate, germinated rice ferment, black bean extract or its hydrolyzate, damask rose flower extract bamboo shoot skin extract, linoleic acid and its derivatives or processed products (e.g. liposomal linoleic acid, etc.), animal or fish-derived collagen and its derivatives, elastin and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.) ), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, Panax ginseng extract or fermented products thereof, Red ginseng extract, honeycomb extract, loofah extract, Ulva extract, peach extract, peach kernel extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, pau d'arco bark extract, hibiscus flower extract products or fermented products, leaf mogul extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, Benifuki extract, silane extract, extract or hydrolyzate of Japanese pepper peel or seed coat, Safflower extract, Casablanca extract, sweet potato extract or fermented product thereof, guava leaf extract, Heutyamia extract, Banpeiyu extract, aloe extract, fig flower extract, apple extract, white asparagus extract etc.

Next, the present invention will be explained in more detail with reference to production examples, test examples, and formulation examples, but the present invention is not limited thereto. In addition, in the following, all parts mean parts by weight, and all percentages mean weight %.

Manufacturing example 1. Fermented lotus seed solution (1)
100 g of lotus seeds (with astringent skin removed) were crushed, 1900 g of purified water was added to prepare a suspension, and the suspension was heat sterilized. This suspension was inoculated with 10 ⁸ lactic acid bacteria (Lactobacillus plantarum)/mL, and cultured stationary at 37° C. for 3 days under a nitrogen stream. After the cultivation was completed, the mixture was heat sterilized and the culture solution was filtered to obtain 1415 g of a solution of lactic acid bacteria fermented lotus seeds (solid concentration 2.53%).

Production example 2. Fermented lotus seed solution (2)
1430 g of a yeast fermented lotus seed solution (solid content concentration 2.24%) was obtained in the same manner as in Production Example 1, except that the yeast Saccharomyces cerevisiae was used as the microorganism in place of lactic acid bacteria (Lactobacillus plantarum).

Production example 3. Fermented lotus seed solution (3)
Aspergillus oryzae, which is a koji mold, was used as the microorganism instead of lactic acid bacteria (Lactobacillus plantarum), but in the same manner as in Production Example 1, 1420 g of a solution of koji mold fermented lotus seeds (solid content concentration 2.41%) was obtained. .

Manufacturing example 4. Fermented lotus seed solution (4)
1385 g of a solution of Bacillus subtilis fermented lotus seeds (solid content concentration 2.60%) was prepared in the same manner as in Production Example 1, except that Bacillus natto, which is Bacillus subtilis, was used as the microorganism instead of lactic acid bacteria (Lactobacillus plantarum). Obtained.

Manufacturing example 5. Lotus whole plant fermented product solution (1)
1167 g of a solution of lactic acid bacteria fermented whole lotus plant (solid content 1.21 %) was obtained.

Production example 6. Rice leaf hydrolyzate solution (1)
Add 1,000 g of purified water to 200 g of dried and crushed rice leaves just before heading (heading stage), extract at 80°C for 1 hour, filter, and prepare 550 g of a pale yellow transparent rice leaf extract solution (solid). 2.5%) was obtained. 0.025 g of pectinase was added to 500 g of the obtained extract solution and hydrolyzed at 40° C. for 4 hours. Thereafter, the mixture was heated at 90° C. for 1 hour to inactivate the enzyme, and then filtered to obtain 455 g of a pale yellow and transparent enzyme hydrolyzate solution of rice leaf extract (solid content concentration: 2.72%).

Manufacturing example 7. Rice leaf hydrolyzate solution (2)
450 g of an enzymatic hydrolyzate solution of rice leaf extract (solid content concentration 2.31%) was obtained in the same manner as Production Example 1 except that cellulase was used instead of pectinase.

Production example 8. Rice leaf hydrolyzate solution (3)
452 g of an enzymatic hydrolyzate solution of rice leaf extract (solid content concentration 2.43%) was obtained in the same manner as Production Example 1 except that xylanase was used instead of pectinase.

Production example 9. Preparation of Salicornia extract (1)
200 g of purified water was added to 20 g of dried shredded whole plant of Salicornia herbacea, and extracted at 40° C. for 1 hour. The obtained extract was filtered to obtain 155 g of a brown transparent extract solution (solid content: 2.00%).

Production example 10. Preparation of Salicornia extract (2)
200 g of purified water was added to 20 g of dried shredded Salicornia stems and extracted at 40° C. for 3 hours. The obtained extract was filtered to obtain 152 g of a brown transparent extract solution (solid content: 1.99%).

Production example 11. Preparation of Salicornia extract (3)
200 g of a 50% aqueous 1,3-butylene glycol solution was added to 20 g of dried shredded whole plants of Salicornia sativa and extracted at 40° C. for 5 hours. The obtained extract was filtered to obtain 160 g of a light brown transparent extract solution (solid content: 1.95%).

Production example 12. Soybean Hydrolyzate 200 g of purified water was added to 10 g of dried and crushed black soybean seeds (black soybean), and extracted at 80° C. for 1 hour. Neurase (manufactured by Amano Enzyme Co., Ltd.) was added to the crudely filtered extract at a concentration of 0.01%, and the mixture was allowed to act at 40° C. for 3 hours. Next, the mixture was treated at 80° C. for 1 hour to inactivate the enzyme, and then filtered to obtain 160 g of a light brown transparent black soybean extract hydrolyzate solution (solid content concentration 1.2%).

Prescription example 1. Emulsion [Ingredients] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Lotus essential oil 0.025
Polyoxyethylene (20) Sorbitan Monostearate 1.0
Lipophilic glyceryl stearate 1.0
Hydrogenated soy lecithin 1.5
Fermented product of Production Example 1 3.0
L-ascorbic acid 2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Water-soluble collagen 0.1
Flavoring: Appropriate amount Purified water: Amount to make 100 parts

Prescription example 2. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 3.0 parts of the fermented product of Production Example 2 contained in Formulation Example 1 was replaced with 3.0 parts of the fermented product of Production Example 2.

Prescription example 3. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 3.0 parts of the fermented product of Production Example 3 contained in Formulation Example 1 was replaced with 3.0 parts of the fermented product of Production Example 3.

Prescription example 4. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 3.0 parts of the fermented product of Production Example 4 contained in Formulation Example 1 was replaced with 3.0 parts of the fermented product of Production Example 4.

Prescription example 5. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 3.0 parts of the fermented product of Production Example 5 contained in Formulation Example 1 was replaced with 3.0 parts of the fermented product of Production Example 5.

Prescription example 6. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate of Production Example 6 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 7. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate of Production Example 7 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 8. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate of Production Example 8 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 9. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 9 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 10. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 10 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 11. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 11 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 12. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate of Production Example 12 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 13. Emulsion: Prescription except that 2.0 parts of L-ascorbic acid-2-phosphate magnesium ester is used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide contained in Prescription Example 1. A milky lotion was obtained in the same manner as in Example 1.

Prescription example 14. Emulsion: Prescription except that 2.0 parts of sodium L-ascorbic acid-2-phosphate ester is used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Prescription Example 1. A milky lotion was obtained in the same manner as in Example 1.

Prescription example 15. Emulsion Same as Formulation Example 1 except that 1.0 part of 3-O-ethyl ascorbic acid is used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide contained in Formulation Example 1. A milky lotion was obtained.

Prescription example 16. Emulsion [Ingredients] Part Squalane 3.0
Behenyl alcohol 3.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) Sorbitan Monostearate 1.0
Glycerin fatty acid ester 1.0
Soybean lecithin 1.5
Fermented product of Production Example 1 5.0
L-ascorbic acid 2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Water-soluble collagen 0.1
Sodium hyaluronate 0.01
Purified water Amount that makes the total amount 100 parts

Prescription example 17. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 16, except that 1.0 part of tranexamic acid was used in place of 1.0 part of dipotassium glycyrrhizinate contained in Formulation Example 16.

Prescription example 18. Lotion [Ingredients] Part Jojoba oil 1.0
Polyoxyethylene (5.5) Cetyl Alcohol 5.0
Butylparaben 0.1
Fermented product of Production Example 1 5.0
L-ascorbic acid 2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide (appropriate amount) Flavor (appropriate amount) Purified water (amount to make 100 parts)

Prescription example 19. Lotion [Ingredients] Part Fermented product of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Guar gum 0.1
Flavoring (appropriate amount) Potassium hydroxide (appropriate amount) Purified water (amount to make 100 parts)

Prescription example 20. Lotion A lotion was obtained in the same manner as in Formulation Example 19, except that 10.0 parts of the hydrolyzate of Production Example 6 was used in place of the fermented product of Production Example 2 contained in the ingredients of Formulation Example 19.

Prescription example 21. Essence [Ingredients] Part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid liquid 0.1
Extract of Production Example 9 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water Amount that makes the total amount 100 parts

Example 22. Liquid foundation [Ingredients] Part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
Fermented product of Production Example 4 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount to make the total amount 100 parts Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount

Prescription example 23. Body shampoo [Ingredients] Part N-lauroylmethylalanine sodium 25.0
Coconut oil fatty acid potassium solution (40%) 26.0
Coconut oil fatty acid diethanolamide 3.0
Methylparaben 0.1
Hydrolyzate of Production Example 7 5.0
1,3-butylene glycol 2.0
Purified water Amount that makes the total amount 100 parts

Prescription example 24. Hair shampoo [Ingredients] Part N-coconut oil fatty acid methyltaurine sodium 10.0
Polyoxyethylene (3) alkyl ether sodium sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Coconut oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Extract of Production Example 10 2.0
1,3-butylene glycol 2.0
Purified water Amount that makes the total amount 100 parts

Prescription example 25. Hair conditioner [Ingredients] Part Polyoxyethylene (10) Hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Setanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
Hydrolyzate of Production Example 8 2.0
1,3-butylene glycol 5.0
Purified water Amount that makes the total amount 100 parts

Prescription example 26. Beverage [Ingredients] Part Hydrolyzate of Production Example 6 5.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Vitamin C 0.1
Purified water Amount that makes the total amount 100 parts

Prescription example 27. Beverage A beverage was obtained in the same manner as Formulation Example 26 except that the hydrolyzate of Production Example 9 was used instead of the fermented product of Production Example 1 contained in Formulation Example 26.

Prescription example 28. Beverage A beverage was obtained in the same manner as Formulation Example 26 except that the hydrolyzate of Production Example 12 was used instead of the fermented product of Production Example 1 contained in Formulation Example 26.

Test example 1. Sodium-dependent vitamin C transporter (SVCT) synthesis promotion effect Normal human epidermal melanocytes were prepared at 1 x ¹⁰ cells/mL using DermaLife (registered trademark) [manufactured by Kurabo Industries, Ltd.] containing a proliferation additive, and plated in a 96-well microplate. 100 μL each was inoculated and cultured at 37°C under 5% carbon dioxide gas and saturated water vapor. After 24 hours, a culture solution containing each of the fermented products of Production Examples 1 and 2, the hydrolysates of Production Examples 6 and 7, and the extracts of Production Examples 9 and 10 as sample solution 1 was added and further cultured. Here, sample solution 1 was prepared so that the final concentration as a solution with respect to the culture solution was 0.25%, 0.5%, and 1.0%. Further, the hydrolyzate of Production Example 12 was prepared as sample solution 2 so that the final concentration as a solution with respect to the culture solution was 2.0%. In addition, as a control, control group 1 was added with a culture solution containing PBS(-) (1.0%) in place of sample solution 1, and control group 1 was added with culture solution containing PBS(-) (2.0%) in place of sample solution 2. A control group 2 was set up in which the culture medium was additionally added. After 48 hours, the culture supernatant was removed, and 200 μL of PBS(-) was added and removed. Next, 50 μL of 10% trichloroacetic acid (Wako Pure Chemical Industries, Ltd.) was added and incubated for 30 minutes in the cold. Afterwards, the supernatant was removed. The plate was washed with 100 μL of PBS(-), and 50 μL of PBS(-) containing 0.2% Triton-X was added and incubated at room temperature for 1 hour. The supernatant was removed, 50 μL of PBS(-) containing 8% bovine serum albumin (SIGMA) was added, and the mixture was incubated at room temperature for 2 hours. The supernatant was removed, the plate was washed with 100 μL of PBS(-) containing 0.2% Triton-X, and 50 μL of anti-SVCT mouse monoclonal antibody (Santa Cruz) was added and incubated in the cold for 24 hours. The supernatant was removed and washing was repeated three times using 100 μL of PBS(-) containing 0.2% Triton-X. 50 μL of Alexa Fluor 488 anti-mouse secondary antibody (Life Technologies) was added and incubated at room temperature in the dark for 2 hours. Remove the supernatant and wash three times using 100 μL of PBS(-) containing 0.2% Triton-X, add 100 μL each of PBS(-), and use Ex485/Em520 using a fluorescence plate reader (Dainippon Pharmaceutical Co., Ltd.). The fluorescence intensity was measured. The relative value of the fluorescence intensity with respect to the measured value in the control group was defined as the SVCT synthesis rate (%).

Table 1 shows the results of Test Example 1 regarding Sample Solution 1.
[Table 1]

As shown in Table 1, it was confirmed that the extract, hydrolyzate, or fermented product according to the present invention has an extremely excellent effect of promoting SVCT synthesis in a concentration-dependent manner.

Table 2 shows the results of Test Example 1 regarding sample solution 2.
[Table 2]

As shown in Table 2, it was confirmed that the hydrolyzate according to the present invention has an extremely excellent effect of promoting SVCT synthesis.

Claims (1)

An agent for promoting vitamin C uptake into cells , which contains as an active ingredient a fermented product of lactic acid bacteria or yeast of lotus seeds , or a fermented product of lactic acid bacteria or yeast of lotus seed extract.