JP2019034899A - External composition for skin - Google Patents

Abstract

To provide an external composition for skin which is derived from natural product excellent in biological safety, has a hyaluronic acid synthetic facilitatory action of epidermic cells and/or an elastase activity inhibitory action, and is used for prevention and improvement of wrinkles and sagging of the skin.SOLUTION: An external composition for skin having a hyaluronic acid synthetic facilitatory action of epidermic cells or an elastase activity inhibitory action for prevention and improvement of wrinkles and sagging of the skin includes an extractable material, hydrolyzate thereof, or a fermentation product thereof from one or more following plants: Oryza sativa of the genera Oryza belonging to Gramineae, bamboo of the genera Bamboo subfamily belonging to Gramineae, adlay of the genera Coix belonging to Gramineae, hibiscus of the genera Hibiscus belonging to Malvaceae, eelgrass or dwarf eelgrass of the genera Zostera belonging to Zosteraceae, eggplant of the genera Solanum belonging to Solanaceae, plant of the genera Tabebuia belonging to Bignoniaceous, soybean of the genera Glycine belonging to Leguminosae, sponge cucumber of the genera Luffa belonging to Cucurbitaceae, plant of the genera Tabebuia belonging to Bignoniaceous, or kiwi of the genera Actinidia belonging to Actinidiaceae.SELECTED DRAWING: None

Description

  The present invention is a novel active ingredient derived from a natural product, which prevents and improves wrinkles (including fine wrinkles) and tarmi, and a composition for external use of skin containing the same.

  Conventionally, it is known that wrinkles and tarmi, which are skin aging phenomena, are caused by ultraviolet ray irradiation, drying, or a decrease in extracellular matrix (collagen, elastin, etc.) due to aging.

  Various active ingredients have been proposed for the purpose of preventing and improving the above-mentioned skin aging phenomenon, and cosmetics, quasi-drugs or pharmaceuticals containing these active ingredients have been put on the market. For example, active ingredients include antioxidants such as vitamin C, vitamin E, catalase, or derivatives thereof, and extracellular matrix components such as collagen, elastin, hyaluronic acid or salts thereof.

  However, these conventional components have problems in terms of stability, safety and effectiveness when actually applied to a living body.

  As a result of diligent research to solve the above-mentioned problems, the present inventors have developed a plant having an epidermal cell hyaluronic acid synthesis promoting action and / or an elastase activity inhibiting action (Gramineae, Amamoaceae, Solanum, Aoiaceae, Nozenaceae, The present inventors have found an extract or a hydrolyzate thereof, or a fermented plant product.

Conventionally, extracts of gramineous plants, hydrolysates or fermented products, extracts of leguminous plants or hydrolysates thereof, extracts of leguminous plants, extracts of solanaceous plants, extracts of mallow The invention using a fermented product, an extract of the genus Cranaceae plant, an extract of the scorpionaceae plant, or an extract of the cucurbitaceae plant as a skin external preparation has been known, for example, from Patent Documents 1 to 11. However, extracts of rice, bamboo or pearl barley, hydrolysates or fermented products, soybean hydrolysates of leguminous plants, fermented products of hibiscus, mallow, Extract of core leg, extract of eggplant which is a solanaceous plant, extract of Tabebia from the family of genus Candaceae, extract of spinach of cucurbitaceae, or extract of kiwi of scorpionaceae promotes hyaluronic acid synthesis in epidermal cells And / or it has not been known to have an elastase activity inhibitory action.
JP 2000-326538 A JP 2011-248559 JP 2013-254639 JP2008-317897 JP 2006-290742 A JP 2006-347925 JP 04-139108 JP2008-069074 JP 2010-059139 JP 61-289010 JP2015-160821

The present invention includes a wrinkle and a tarmi containing an extract of one or more of the following (A) to (G) or a hydrolyzate thereof, or a fermented product of the plant, and having an epidermal cell hyaluronic acid synthesis promoting action. It is a composition for external use for prevention and improvement of skin.
(A) a hydrolyzate of rice belonging to the genus Gramineae;
(B) Extract of bamboo shoots from the family Bambooaceae
(C) a fermented pearl barley belonging to the genus Giusedaceae or an extract thereof;
(D) a fermented product of Hibiscus of the genus Hibiscus or its extract;
(E) An extract of the genus Amamo or core eel of the genus Amamoaceae;
(F) Eggplant extract of the genus Solanum genus;
(G) An extract of a plant belonging to the genus Tabebuia belonging to the genus Nozenaceae The present invention includes a wrinkle having an elastase activity inhibitory action, comprising an extract of any one or more of the following (A) to (D) or a hydrolyzate thereof: And an external composition for the prevention and improvement of talmi.
(A) Soybean hydrolyzate of the leguminous soybean genus;
(B) an extract of loofah of the genus Cucurbitaceae;
(C) Kiwi extract of the genus Matatabidae.
(D) Plant extract of the genus Tabebia

  Since the extract, hydrolyzate or fermented product according to the present invention has an epidermal cell hyaluronic acid synthesis promoting action and / or an elastase activity inhibiting action, wrinkles and tarmi can be prevented and improved, and further, pore care (for pores) It is useful as an active ingredient for suppressing spread.

The best mode for carrying out the present invention will be described below, but the present invention is not limited to this.
First, as the "rice" of the Poaceae rice genus (Oryza) used in the present invention, any of those belonging to Oryza sativa can be used for taxonomics, specifically, for example, Koshihikari, Sasanishiki, Nihonbare, Akita Komachi, Kinuhikari, Hua Echizen etc. can be mentioned, but of course it is not limited to these. In the present invention, the use site of rice is not particularly limited, and any suitable site such as whole grass, leaves, flowers, stems, roots, seeds (grains) can be used, but whole grasses and leaves are preferred. Moreover, when using a leaf, it is more preferable to use the leaf before heading. In addition, when using a rice leaf, you may heat-process a leaf before an extraction process. Thereby, the modification | denaturation and alteration by the active component (enzyme etc.) contained in a rice leaf can be suppressed, and the activity fall during storage of the extract | collected rice leaf can be prevented.

  Examples of the “bamboo” of Bambusoideae used in the present invention include, for example, mushroom (Phyllostachys bambusoides), mushroom (Phyllostachys pubescens), bee (Pyllostachys nigra), pyllostachys ayys (Phyllostachys ayla), , Bambusa multiplex, Semiarundinaria fastuosa, Sasa kurilensis, Sinobambusa tootsik, ii, Chimonobambusa marmorep, Chimonobambusa marmorep, Chimonobambusa marmorep However, the present invention is not limited to this. In the present invention, it is preferable to use bamboo shoots (bamboo shoots) that are young shoots of bamboo. The portion of the bamboo shoots may be an edible portion, a skin portion of a non-edible portion, or bamboo water. Bamboo shoots refer to young shoots of the bamboo of the family Gramineae. Preferred bamboo shoots used in the present invention vary depending on the seed of the bamboo. For example, in the case of mushrooms, those harvested from June to September, preferably those having a ground part of 10 to 500 cm, and further from the ground part. The thing of 30 cm-200 cm is more preferable. Moreover, in the case of Mosouchiku, the thing until a young shoot appears on the ground harvested from the beginning of March to the end of June is preferable. If it grows beyond the above range, the edible part and the skin of the young shoots become hard, and the amount of tyrosine which is undesirable as a component of cosmetics increases, so that it is not suitable for the present invention.

  Examples of the plant of the genus Zosteraceae (Zostera) used in the present invention include, for example, Zostera marina, Zostera japonica, Zostera asiatica, Zostera caespitosa, and Zostera caulescens. In the present invention, among the plants of the genus Amamo, amamo or core malam (preferably whole grass) is preferable from the viewpoint of easy material acquisition.

  Soybean used as a raw material in the present invention is a plant classified into the same species as soybean (scientific name: Glycine max (L.) Merill) in plant taxonomy. As the soybean, any of white soybean (yellow soybean), green soybean, black soybean, and the like can be used.

  Examples of “Hibiscus” of the genus Hibiscus used in the present invention include, for example, Roselle (Hibiscus sabdariffa L.), Mucuge (Hibiscus syriacus), Fubis (Hibiscus mutabills), Momijia (Hibiscus coccineus), (Hibiscus tiliaceus), Bussougi (Hibiscus schizopetalus) and the like. Examples of the use site include whole grass, leaves, flower parts, stems, roots and grains. In addition, when using a flower part, the flowering time, a magnitude | size, etc. of a flower are not specifically limited, Moreover, you may use the thing containing either or all of a petal, a bud, etc.

  In the present invention, the eggplant is an eggplant belonging to the genus Solanum, for example, long eggplant, water eggplant, Kamo eggplant, Yamatomaru eggplant or the like.

  The barley used in the present invention is a barley of the genus Coix, and the use of barley seeds is preferred. In the present invention, when using pearl barley seeds, both those with shells and those without shells can be used, and further, powders obtained by pulverization or crushing, or grains of pearl barley seeds. In any case, a fermented product that is equivalent and has a stronger skin physiological activity than the original pearl seeds can be obtained. From the viewpoint of extraction / fermentation efficiency, it is preferable to use a powder obtained by pulverization or crushing, or a high-temperature / high-pressure processed product thereof, in both cases of shelled and shell-removed products.

  Examples of the plant belonging to the genus Tabigniaceae (Bignoniaceae) used in the present invention include, for example, Tabebuia impetiginosa, Tabebuia rosea, Tabebuia caraicia and Tabebuia caraicia. Tabebuia chrysantha), Tabebuia chrysotricha and the like, but the present invention is not limited to these. Further, in the present invention, the whole plant or bark (inner bark) of the Tabebia genus plant is suitably used as an extraction raw material. Of these plants belonging to the genus Tabebuia, the use of tabebuia impetigenosa, particularly the use of internal bark, is most preferable from the viewpoint of the effectiveness of the extract. Tabebuia Impetiginosa is called Paudalco, Ipe, Iperoscio, Rapacho, Tahibo, etc. in Brazil. Synonyms include T. avellanedae and T. ipe.

  When preparing an extract from the above plant, it can carry out as follows, for example. That is, first, the extraction site of each plant is preliminarily washed with water if necessary to remove foreign substances, and then directly or dried, and then minced or pulverized as necessary, and extracted by contacting with an extraction solvent. The extraction can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method, but a supercritical extraction method or a steam distillation method can also be used.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; ethylene glycol, 1,3-propanediol, 1,3-butylene glycol, Polyhydric alcohols such as glycerol; Esters such as ethyl acetate, butyl acetate, methyl propionate and glyceryl trioctanoate; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether, isopropyl and ether; n-hexane, toluene And hydrocarbon solvents such as chloroform and the like, and these can be used alone or in admixture of two or more.

  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 by volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1: if the mixed solvent is water and glycerin. In the case of 1 to 15: 1, or a mixed solvent of water and 1,3-propanediol or 1,3-butylene glycol, a range of 1: 1 to 15: 1 is preferable.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent. If so, the extraction temperature is preferably in the range of 0 ° C. to 90 ° C., and the extraction time is preferably 0.5 hours to 7 days.

  Here, prior to the extraction process of the present invention or in parallel with the extraction process, the extraction site may be subjected to a hydrolysis process as necessary. Thereby, there is a possibility that the extract can be used more effectively by improving the skin irritation, effectiveness or storage stability of the extract.

  In particular, in the present invention, it is preferable to subject the rice leaf and soybean extract to a hydrolysis treatment. Examples of the hydrolysis treatment method include acid, alkali or enzyme decomposition treatment methods.

  When the hydrolysis treatment is performed using an enzyme, at least one enzyme selected from a proteolytic enzyme, a amylolytic enzyme, a pectin degrading enzyme, a fibrinolytic enzyme, and a lipolytic enzyme can be used as the enzyme. .

  Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, and peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase. , Bromelain, microorganism-derived complex proteolytic enzymes (for example, neurase [manufactured by Amano Enzyme Co., Ltd.]) and the like can be used.

  As the starch degrading enzyme, for example, α-amylase, β-amylase, glucoamylase, β-galactosidase and the like can be used.

  As the pectin degrading enzyme, for example, pectinase, pectin depolymerase, pectin demethoxylase, pectin lyase, pectin esterase, polygalacturonase and the like can be used.

  Examples of the fibrinolytic enzyme include cellulase, hemicellulase, agarase, mannase, chitinase, chitosanase, carrageenase, arginase, fucoidanase, inulase, xylanase, and ligninase.

  As the lipolytic enzyme, for example, lipase, phospholipase and the like can be used.

  In addition, the present invention may be subjected to a fermentation treatment with a microorganism (such as lactic acid bacteria, yeast, bacilli or Bacillus subtilis) for the purpose of improving the effectiveness of the extraction material, suppressing skin irritation or improving stability. In particular, in the present invention, hibiscus and pearl barley or an extract thereof are preferably subjected to fermentation treatment with microorganisms.

  When performing fermentation, it can carry out as follows, for example. First, as an assimilation source for fermentation, the plant itself (hereinafter sometimes referred to as a plant body) may be used, or an extract obtained by extracting the plant body with an appropriate medium may be used. Moreover, when using an extract, it is also possible to perform fermentation with a plant body contained, without removing the plant body of an extract by solid-liquid separation. Here, the plant may be raw or may be previously dried or semi-dried. Further, the collected shape can be used as it is.

  In the present invention, lactic acid bacteria are, for example, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, Lactobacillus delbrueckii Lactobacillus delbrueckii and other Lactobacillus genus Lactobacillus genus Lactobacillus Lactic acid bacteria of the genus Carnobacterium such as Carnobacterium divergens, Carnobacterium piscicola; Leuconostoc mesenteroides, Leuconostoc lactis, Leuconostoc lactis Leuconostoc lactic acid bacteria such as Leuconostoc citreum; Streptococcus faecalis, Streptococcus pyogenes (St Leptococcus pyogenes) and other Streptococcus lactic acid bacteria; Enterococcus caseliflavus, Enterococcus sulfreus and other Enterococcus lactic acid bacteria; Lactococcus lacta Lactic acid bacteria of the genus Lactococcus; lactic acid bacteria of the genus Weissella such as Weissella confusa and Weissella kandleri; Lactic acid bacteria of the genus Vagococcus (Vagococcus fluvialis), Vagococcus salmoninarum, etc .; Pedi Lactococcus Damunosasu (Pediococcus damnosus), include lactic acid bacteria of the Pediococcus (Pediococcus) genus such as Pediococcus pentosaceus (Pediococcus pentosaceus).

  Further, in the present invention, yeast refers to, for example, Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carls bergensis, Yeast of the genus Saccharomyces, yeast of the genus Galactomyces, Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei, Torulaspora rosei, etc. Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, Digosaccharomyces miso Yeasts of the genus Zygosaccharomyces lactis such as Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, and Candida scotty Yeasts of the genus Aureobasidium such as Aureobasidium pullulans, Aureobasidium mansonii, Aureobasideium microstictum, etc. . The yeast according to the present invention may be any of sake yeast, wine yeast, beer yeast, plant flower (rose, lily, cherry tree, etc.) yeast, or sea-derived yeast.

  Further, in the present invention, aspergillus refers to, for example, Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus sojae, etc. ), Aspergillus kawauchii, Aspergillus usami, Aspergillus niger, etc. Aspergillus niger, Monascus anka, Monascus pilosus, etc.

  In the present invention, examples of Bacillus subtilis include Bacillus natto, Bacillus subtilis, and Bacillus circulans.

  When the above-mentioned suspension or extract is fermented with microorganisms, it is necessary to sterilize the microorganisms before the fermentation step to remove the germs that hinder fermentation. As a method for sterilizing and removing the various bacteria, a method in which the fermentation material is washed with ethanol for sterilization in advance and then suspended in a sterile solvent such as sterile water may be used. The turbid liquid may be sterilized by heat sterilization or the like. As the heat sterilization treatment, the autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, or holding at 80 to 90 ° C. for 60 to 120 minutes is repeated once a day for 2 to 3 days. The heat sterilization method such as the method is generally used.

The sterilized suspension is placed in a fermentation tank, in which microorganisms are inoculated and fermented. The appropriate amount of inoculated microorganism is 10 ⁷ to 10 ⁸ cells / mL. Even if the inoculation amount exceeds the above range, the fermentation progress time hardly changes. On the other hand, if the inoculation amount is less than the above range, it takes a long time to complete the fermentation.

  The fermentation temperature is generally in the range of 5 to 50 ° C., preferably 20 ° C. to 40 ° C., which is the optimum temperature for growth of each microorganism (for example, 30 ° C. to 40 ° C. for lactic acid bacteria, 25 ° C. to 30 ° C. for yeast). Range. The number of days of fermentation is generally in the range of 1 to 10 days, preferably 2 to 5 days, at the optimum temperature. If the fermentation days are shorter than the above general range, the fermentation is not sufficiently performed and the effectiveness of the fermented product tends to be reduced. On the other hand, if the fermentation days are longer than 10 days, no further increase in effectiveness is observed. Not only that, but also coloring and an increase in fermentation odor occur.

  When preparing a fermented product from hibiscus and pearl barley, the above suspension or extraction is performed before or simultaneously with the inoculation of microorganisms so that the components of the target use site can be used more effectively as a source of microorganism utilization. The above-described hydrolysis treatment may be performed on the physical solution.

  The extract, hydrolyzate or fermented product prepared as described above may be used as it is, generally after adjusting the pH to 3 to 9, or at a desired concentration by vacuum concentration or the like. May be used as Moreover, it is good also as a dried material by conventional methods, such as a spray-dry method.

  In addition, the extract, hydrolyzate, or fermented product prepared as described above may be stored after refrigeration for a certain period of time in order to improve storage stability and the like, and then the supernatant may be used.

  The extract, hydrolyzate or fermented product according to the present invention can be blended in a skin external preparation (cosmetics, quasi-drug, external medicine). Examples of skin external preparations include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, blushers, white powders, facial cleansers, body shampoos, scalp, hair shampoos, hair conditioners. , Shampoos or tonics for hair growth, hair restoration, and cosmetics for cleaning such as soap, and bath preparations, and the like, but the present invention is not limited thereto.

  The blending amount of the extract, hydrolyzate or fermented product according to the present invention can be appropriately adjusted according to the formulation to be blended. As the solid content, for example, in the case of basic cosmetics, 0.002 to 1.0. In the case of makeup cosmetics, in the range of 0.002 to 1.0% by weight, and in the case of cleansing cosmetics, 0.002 to 10.0. It is in the range of wt%. Moreover, in the case of hair cosmetics, it is 0.0001 to 5.0 weight% as solid content of a composition.

  In preparations containing the extract, hydrolyzate or fermented product according to the present invention, in addition to the essential component extract, hydrolyzate or fermented product, components usually used for external preparations for skin and oral compositions, For example, oily components, surfactants (synthetic and natural products), moisturizers, thickeners, antiseptics / bactericides, powder components, antioxidants, chelating agents, pH adjusters, pigments, fragrances, etc. are required Depending on the case, it can be blended appropriately. Moreover, as long as the effectiveness and characteristics are not impaired, there is no problem even if it is used in combination with other physiologically active ingredients.

  Here, as the oil component, for example, lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cacao oil, meadow foam oil, Oils derived from plants such as rosehip oil, lambender oil, sheer butter, tea tree oil, avocado oil, macadamia nut oil, plant-derived squalane; oils derived from animals such as mink oil and turtle oil; beeswax, carnauba wax, rice Waxes such as wax and lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and eicosenoic acid; lauryl alcohol, cetanol, Higher alcohols such as stearyl alcohol; Isopro myristate Le, isopropyl palmitate, butyl oleate, 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  As the surfactant, for example, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, Nonionic surfactants such as polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates , Polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene alkyl phenyl ether phosphate, etc. Surfactant; quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers or emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and saccharides such as trehalose, mucopolysaccharides (for example, Hyaluronic acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) ) Extracts, various amino acids and their derivatives.

  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae, or red algae; silane root (white) extract; pectin, locust bean gum, aloe polysaccharide, alkaline-genes-producing polysaccharide, etc. Polysaccharides such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluron Examples include acid and derivatives thereof; polyglutamic acid and derivatives thereof.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, chloride Benzalkonium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), polyphosphoric acid, propanediol, 1,2-pentanediol, various essential oils, dry bark, radish fermented liquor, sugarcane, etc. There are plant-derived ethanol and 1,3-butylene glycol.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, Cellulose powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, red beans, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy- 4-methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. is there.

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, murasakixibub extract, silane root extract, peony extract, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate) Etc.).

  Examples of the chelating agent include trisodium ethylenediaminehydroxyethyl triacetate, edetic acid or a salt thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, tetrasodium hydroxyethane diphosphonate, and the like.

  Examples of the pH adjuster include citric acid or a salt thereof, lactic acid or a salt thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide and the like.

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof 4-methoxysalicylic acid potassium salt, magnolignan (5,5′-dipropyl-biphenyl-2,2′-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine mono) Phosphate, adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivative include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid esters such as kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. As the ascorbic acid derivative, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid- Ascorbic acid ester salts such as 2-sulfate magnesium, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleic acid, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbic acid Ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L -L-ascorbic acid tetrafatty acid esters such as ascorbyl tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acyl thereof Derivatives, ascorbyl glycerol derivatives such as bisglyceryl ascorbic acid, aminopropyl phosphate L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl Examples of hydroquinone derivatives include phosphates such as arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives such as tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionylol. Examples of nicotinic acid derivatives include nicotinic acid amide and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. .

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus flower extract, pearl barley hydrolyzate, royal jelly fermented product, sake lees extract or ceramide contained therein, fermented sake lees, pandanus amaryfoliius (Pandanus amaryllifolius Roxb.) Extract, Arcangelicia flava Merrilli extract, chamomile extract and the like. Also, apricot fruit extract, seaweed extract such as catamen giraffe, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, rice fermented extract, germinated rice extract or hydrolyzate thereof, germinated rice fermented , Damask rose flower extract, linoleic acid and derivatives or processed products thereof (eg liposomal linoleic acid), animal or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt) Etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or fermented product thereof , Red ginseng extract, beetle extract, anaaaosa extract, peach extract Extracts, peach seed extract, sunflower extract, Zizyphus joazeiro extract, hibiscus flower extract or fermented product, hagoromogusa extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract , Red rich extract, silane extract, yam peel or seed extract or hydrolyzate, safflower flower extract, Casablanca extract, sweet potato extract or fermented product thereof, guava leaf extract, dokudami extract, evening Examples include birch extract, aloe extract, fig flower extract, apple extract, white asparagus extract.

  Next, although a manufacture example, a test example, and a formulation example are given and this invention is demonstrated further more concretely, this invention is not limited to them. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of rice leaf hydrolyzate (1)
Purified water (1000 g) was added to 200 g of dry pulverized rice leaves immediately before heading (blossoming stage), extracted at 80 ° C. for 1 hour, filtered and 550 g of light yellow transparent rice leaf extract solution (solid) A partial concentration of 2.5%). To 500 g of the obtained extract solution, 0.025 g of pectinase was added and hydrolyzed at 40 ° C. for 4 hours. Thereafter, the enzyme was inactivated by heating at 90 ° C. for 1 hour, followed by filtration to obtain 457 g (solid content concentration: 2.73%) of an enzyme hydrolyzate solution of light yellow transparent rice leaf extract.

Production Example 2 Preparation of rice leaf hydrolyzate (2)
449 g of enzyme hydrolyzate solution of rice leaf extract (solid content concentration 2.30%) was obtained in the same manner as in Production Example 1 except that cellulase was used instead of pectinase.

Production Example 3 Preparation of rice leaf hydrolyzate (3)
In the same manner as in Production Example 1, except that xylanase was used instead of pectinase, 454 g (solid content concentration: 2.51%) of a rice leaf extract enzyme hydrolyzate was obtained.

Production Example 4 Preparation of bamboo shoot extract (1)
To 100 g of dried crushed bamboo shoots of bamboo shoots (Phyllostachys bambusoides), 1000 g of purified water was added, extracted at 50 ° C. for 3 hours, the extract was filtered, and 657 g of light brown transparent madake extract (solid content 0) 8%).

Production Example 5 Preparation of bamboo shoot skin extract (2)
An extract solution was prepared in the same manner as in Production Example 2 except that Moso bamboo was used instead of the mushrooms in Production Example 2 as a bamboo shoot, and 521 g of a light brown bamboo shoot peel extract solution was obtained (solid content concentration 0. 5%).

Production Example 6 Preparation of fermented pearl barley seeds (1)
100 g of lotus seeds (those with astringent skin removed) were pulverized, 1900 g of purified water was added to prepare a suspension, and heat sterilized. After adding 1 g of glucoamylase, 1 g of papain, and 0.5 g of cellulase to this suspension, 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum) were inoculated, and left to stand at 37 ° C. for 3 days in a nitrogen stream. . After completion of the culture, the mixture was sterilized by heating, and the culture solution was filtered to obtain 1450 g of a lotus seed fermented lactic acid bacterium solution (solid content concentration: 2.7%).

Production Example 7 Preparation of fermented pearl barley seeds (2)
50 g of barley seeds without the shell were pulverized, 950 g of purified water was added to prepare a suspension, and heat sterilized. After adding 0.5 g of glucoamylase and 0.5 g of papain to this suspension, 10 ⁷ yeast / mL of yeast (Saccharomyces cerevisiae) was inoculated, followed by stationary culture at 30 ° C. for 3 days. After completion of the culture, the mixture was sterilized by heating, cooled to room temperature, and filtered to obtain 520 g of a fermented pearl seed solution (solid concentration: 1.4%).

Production Example 8 Preparation of eelgrass extract Fresh eelgrass (Zostera marina) whole plant was thoroughly washed, dried in the sun and chopped. 20 g of this dried product was extracted for 7 days at room temperature using 1 kg of a solvent of water and ethanol (volume ratio 100: 7). This was filtered, and powdered activated carbon was added at 2% by weight of the filtrate and stirred for 10 minutes. This liquid was filtered to obtain 810 g of a pale yellow processed product (solid content 0.15% by weight).

Production Example 9 Preparation of water eggplant juice liquid 2800 g of eggplant (water eggplant) fruit was made into a paste using a food processor, and the resulting liquid was further filtered to obtain 2010 g of tan brown clear water eggplant juice liquid (solid) Minor concentration 4.05%).

Production Example 10 Preparation of water eggplant fruit extract 100 g of dried water eggplant fruit was mixed with 1000 g of purified water, extracted at 40 ° C. for 4 hours, and then filtered to obtain 810 g of a yellow clear water eggplant fruit extract (solid Minor concentration 1.53%).

Production Example 11 Preparation of fermented hibiscus (1)
A suspension was prepared by adding 950 g of purified water to 50 g of flowers of Hibissucas sabdariffa L., and sterilized by heating at 80 ° C. for 1 hour. The sterilized suspension was inoculated with 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum), and statically cultured at 37 ° C. for 3 days. After completion of the culture, the culture solution was sterilized by heating and filtered to obtain 730 g of a lactic acid bacteria fermented product solution (solid content concentration: 3.21%).

Production Example 12. Preparation of fermented hibiscus (2)
655 g (solid content concentration: 2.71%) of a fermented lactic acid bacteria solution of Fussa (Hibiscus) genus Mucuge was obtained in the same manner as in Production Example 1 except that Hibiscus syriacus was used instead of Roselle.

  Production Example 13 In the same manner as in Production Example 13 except that Saccharomyces cerevisiae, which is yeast, was used instead of lactic acid bacteria, 810 g of a yeast fermented yeast solution of Roselle koji (solid content concentration: 3.22%) was obtained.

Production Example 14 Preparation of Tabebuia Impetiginosa Extract 100g of Tabebuia Impetiginosa's internal bark was mixed with 1000g of purified water, extracted at 4 ° C for 24 hours, filtered, and light brown transparent extract 655 g of a solution was obtained (solid content concentration 1.51%). This was diluted 6 times with purified water to obtain an extract solution.

Production Example 15. Preparation of soybean hydrolyzate (1)
200 g of purified water was added to 10 g of dry pulverized black soybean seeds and extracted at 80 ° C. for 1 hour. After roughly filtering the obtained extract with pH 5 and using diluted hydrochloric acid, lipase and protease (papain) were added to a concentration of 0.01% and allowed to act at 40 ° C. for 3 hours. Next, it was treated at 80 ° C. for 1 hour to deactivate the enzyme, followed by filtration to obtain 165 g of a hydrolyzate solution (solid content concentration: 1.24%) of a light brown transparent black soybean extract.

Production Example 16. Preparation of soybean hydrolyzate (2)
In Production Example 15, a light yellow transparent soy extract hydrolyzate solution (solids concentration 1.09) was obtained in the same manner as in Production Example 1 except that white soybean seeds were used instead of black soybean seeds. %) 160 g was obtained.

Production Example 17 Preparation of kiwi fruit extract 300 g of immature fruit of kiwi (Actinidia chinensis) is minced, and 50 g of purified water is added thereto. Extraction with the purified water is performed overnight in the refrigerator. The extract was filtered to obtain 315 g of a light brown transparent kiwi immature fruit extract (solid content concentration 2.1%).

Production Example 18. Preparation of loofah extract (1)
100 g of purified water was added to 10 g of dried luffa fruits and stems and leaves, and extracted at 40 ° C. for 3 hours. The obtained solution was filtered to obtain 74.0 g (solid content concentration 2.65%) of a brown transparent solution. This was used as a loofah extract solution.

Production Example 19. Preparation of loofah extract (2)
After cutting 200 g of raw luffa fruits, the juice was squeezed and the resulting solution was heated at 40 ° C. for 1 hour. After heating, it was filtered to obtain 120 g (solid concentration: 4.50%). Diluted twice with purified water to obtain a loofah pressed extract solution.

Formulation Example 1 Lotion [Ingredients] part Jojoba oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
Hydrolyzate of Production Example 1 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide appropriate amount Purified water Amount to make 100 parts in total Perfume Appropriate amount

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate in Production Example 2 was used instead of the hydrolyzate in Production Example 1 contained in Formulation Example 1.

Formulation Example 3 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate in Production Example 3 was used instead of the hydrolyzate in Production Example 1 contained in Formulation Example 1.

Formulation Example 4 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 4 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 5 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 5 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 6 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 6 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 7 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 7 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 8 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 8 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 9 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the juice of Production Example 9 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 10 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 10 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 11 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the fermented product of Production Example 11 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 12. Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 12 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 13 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the fermented product of Production Example 13 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 14. Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 14 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 15. Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the hydrolyzate of Production Example 15 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 16. Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate in Production Example 16 was used instead of the hydrolyzate in Production Example 1 contained in Formulation Example 1.

Formulation Example 17. Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 17 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 18. Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 18 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 19. Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 19 was used instead of the hydrolyzate of Production Example 1 contained in Formulation Example 1.

Formulation Example 20. Latex [Ingredients] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Lotus essential oil 0.025
Polyoxyethylene (20) sorbitan monostearate 1.0
Lipophilic glyceryl stearate 1.0
Hydrogenated soybean lecithin 1.5
Extract of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Water-soluble collagen 0.1
Purified water Total amount of 100 parts Perfume Appropriate amount

Formulation Example 21. Emulsion An emulsion was obtained in the same manner as in Formulation Example 20, except that 2.0 parts of tranexamic acid was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 20. It was.

Formulation Example 22. Emulsion An emulsion was obtained in the same manner as in Formulation Example 20, except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide contained in Formulation Example 20. .

Formulation Example 23. Emulsion In the same manner as in Formulation Example 20, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 20 were used, the emulsion was prepared. Obtained.

Formulation Example 24. Emulsion [Ingredients] part squalane 3.0
Behenyl alcohol 3.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 1.0
Glycerin fatty acid ester 1.0
Soy lecithin 1.5
Extract of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Water-soluble collagen 0.1
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 25. Emulsion An emulsion was obtained in the same manner as in Formulation Example 24 except that 1.0 part of tranexamic acid was used instead of 1.0 part of dipotassium glycyrrhizinate contained in Formulation Example 24.

Formulation Example 26. Lotion [component] part Extract of Production Example 4 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Guar gum 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Formulation Example 27. Lotion A lotion was obtained in the same manner as in Formulation Example 26 except that 10.0 parts of the fermented product of Production Example 6 was used instead of the extract of Production Example 4 contained in Formulation Example 26.

Formulation Example 28. Essence [Ingredients] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid solution 0.1
Extract of Production Example 8 5.0
Citric acid 0.3
Sodium citrate 0.6
Amount of purified water totaling 100 parts

Example 29. Liquid foundation [ingredient] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
Extract of Production Example 6 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Titanium oxide 8.0
Talc 4.0
Color pigment Appropriate amount Purified water Amount that makes 100 parts

Test Example 1 Epidermal cell hyaluronic acid synthesis promoting effect Human epidermal cells NHEK were seeded at 4 × 10 ³ cells / well in a 96-well microplate containing HuMedia KG2 medium (manufactured by Kurabo Industries), under conditions of 37 ° C. and 5.0% CO ₂ . After pre-culturing for 1 day, a HuMedia KG2 medium containing the extract, hydrolyzate or fermented product of Production Examples 1 to 14 as a sample solution was added, and further cultured under the same conditions for 3 days. Thereafter, the supernatant was collected from each well, and the amount of hyaluronic acid in the supernatant was quantified using a hyaluronic acid measurement kit [Quantikine (registered number) ELISA Hyaluronan Immunoassay, R & D SYSTEMS, USA]. For cells, after washing once with PBS (-), 100 μL / well of hoechst33342 reagent diluted 100-fold with PBS (-) was added, incubated at 37 ° C for 1 hour, and DNA was fluorescently stained . Thereafter, the fluorescence intensity (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader (Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)) was measured to determine the amount of DNA. The amount of hyaluronic acid synthesized per epidermal cell DNA was calculated by dividing the amount of hyaluronic acid (ng / mL) in the supernatant thus obtained by the amount of DNA. Using the group with PBS (-) added instead of the sample solution as the control, calculate the relative value of the amount of hyaluronic acid synthesis in each group with respect to the amount of hyaluronic acid synthesized in the control group, and promote hyaluronic acid synthesis (%) Asked. In order to confirm that the test was conducted normally, a group to which 10 mM N-acetyl D-glucosamine was added was also set as a positive control.

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, it was confirmed that the extract, hydrolyzate, or fermented product according to the present invention has an excellent epidermal cell hyaluronic acid synthesis promoting effect.

Test Example 2 Neutrophil Elastase Colorimetric Drug Discovery Kit (Enzo Life Sciences Inc. USA) was used for the evaluation test of the neutrophil elastase activity inhibitory effect. According to the protocol attached to the kit, in a 96-well microplate, Assay Buffer 65 μL per well, Elastase (90-fold diluted with Assay Buffer) 10 μL, sample solution (extract or hydrolyzate of Preparation Examples 14, 16-18) Add 20 μL of the solution in Assay Buffer to a final concentration of 0.5, 1.0, or 2.0%), and add 5 μL of the substrate solution (MeOSuc-AAPV-pNA: 10-fold diluted with Assay Buffer) from above. The reaction was started. The assay buffer and substrate solution only group is the blank group, the group with assay buffer added instead of the sample solution is the control group, and the group with Elastatinal adjusted to a final concentration of 0.1 mM is added instead of the sample solution is the positive control group. did. With the substrate solution added to all wells and starting the reaction, a 96-well plate was placed in a microplate reader, and the increase in ABS405nm per minute for 10 minutes was measured in Kinetic mode, and the slope was calculated. . The average value of the slope of the blank group was subtracted from all the wells, and the relative value of each sample addition group with the average value of the control group as 100 was determined to obtain the elastase activity rate (%).

The results of Test Example 2 are shown in Table 2.
[Table 2]

As shown in Table 2, it was confirmed that the extract and the hydrolyzate according to the present invention have an excellent neutrophil elastase activity inhibitory action.

Claims (2)

Prevention or improvement of wrinkles and tarmi containing epidermal cell hyaluronic acid synthesis promoting action, comprising an extract of one or more of the following (A) to (G) or a hydrolyzate thereof, or a fermented product of the plant: Skin external composition.
(A) a hydrolyzate of rice belonging to the genus Gramineae;
(B) Extract of bamboo shoots from the family Bambooaceae
(C) a fermented pearl barley belonging to the genus Giusedaceae or an extract thereof;
(D) a fermented product of Hibiscus of the genus Hibiscus or its extract;
(E) An extract of the genus Amamo or core eel of the genus Amamoaceae;
(F) Eggplant extract of the genus Solanum genus;
(G) Extract from the plant of the genus Tabebuiaceae A skin external composition for preventing and improving wrinkles and tarmi having an elastase activity inhibitory action, comprising an extract of any one or more of the following (A) to (D) or a hydrolyzate thereof:
(A) Soybean hydrolyzate of the leguminous soybean genus;
(B) an extract of loofah of the genus Cucurbitaceae;
(C) Kiwi extract of the genus Matatabidae.
(D) Plant extract of the genus Tabebia