JP2016108261A - Skin turnover improving agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a functional material derived from natural products with excellent biosafety, the functional material improving skin turnover in order to maintain the skin in a youthful and healthy state and in order to make the skin smooth.SOLUTION: The invention provides a skin turnover improving agent containing as an active ingredient a solvent extract of the flowers of Rosa damascena which is a plant belonging to Rosaceae Rosa, the agent having chymotrypsin type serine protease (kallikrein) activity-enhancing action.SELECTED DRAWING: None

Description

  The present invention relates to a skin turnover improving agent obtained from a plant belonging to the genus Rosaceae and having excellent skin physiological activity and biosafety.

  When the human epidermis is functioning normally, the birth of new cells in its basal layer and the loss of old keratinocytes from the stratum corneum are maintained in a certain balance (skin turnover is functioning normally). ) This epidermis turnover is caused by internal factors such as aging, cell / tissue damage caused by active oxygen induced by chemical substances (nitrogen compounds, sulfur compounds, etc.) contained in sunlight (ultraviolet rays), exhaust gas, etc. It is known that it does not function normally due to external factors such as dryness or inflammation.

  Conventionally, γ-aminobutyric acid, diisopropylamine dichloroacetate, or hydroxycarboxylic acid has been proposed as an agent for improving skin turnover, but there are problems in terms of skin safety and effectiveness, and skin safety. There is also a need for an agent for improving skin turnover that is highly effective.

  In view of the problems of the prior art, the present inventors have intensively studied to find a new active ingredient derived from a natural product from the viewpoint of skin safety. As a result, it has been newly found that a solvent extract of Damask rose belonging to the genus Rosaceae has an excellent skin turnover improving effect.

  Conventionally, it has been disclosed in Patent Documents 1 to 3 that a rose extract belonging to the genus Rosaceae has a serine protease activity inhibitory action, a matrix metalloprotease-9 inhibitory action or a hyaluronic acid production promoting effect. Further, it is disclosed in Patent Document 4 that the extract of damask rose can be used as an antibacterial agent or deodorant, and further, the use of a steam distillate of damask rose (such as essential oil) for cosmetics. And Patent Documents 5 and 6.

JP 2000-327555 A Japanese Patent Laid-Open No. 2001-064192 JP 2001-163794 A Japanese Patent Laid-Open No. 2001-316277 JP 2004-175678 A JP 2006-056902 A

  However, it has not been known that the solvent extract of damask rose has an excellent skin turnover improving effect.

  The present invention is a skin turnover improving agent comprising a solvent extract of Damask rose belonging to the genus Rosaceae as an active ingredient.

  INDUSTRIAL APPLICABILITY The present invention is a cosmetic comprising as an active ingredient a solvent extract of damask rose, which is a plant belonging to the genus Rosaceae, and can provide a skin turnover improving agent excellent in biosafety and effectiveness.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The extraction material used in the present invention is a damask rose belonging to the genus Rosaceae (Rosa damascena), and any variety (including hybrids and subspecies) may be used as long as it is a damask rose.

  The material used in the present invention may be any of damask rose whole grass, leaves, flower parts, stems, seeds, fruits, roots, etc., but the use of whole grasses or flower parts is preferred.

  For the preparation of the extract, damask roses (for example, whole grass, flower parts, etc.) are washed with water beforehand if necessary to remove foreign substances, and then left as it is or dried, and then chopped or crushed as necessary. Extraction is performed by contacting with an extraction solvent. Extraction can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method, but a supercritical extraction method can also be used in addition to the immersion method.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, from the viewpoint of skin irritation and effectiveness, and also because they can be widely applied to cosmetics, in the present invention, water, lower alcohols, polyhydric alcohols, and the like are used. A hydrophilic solvent is preferred. Preferable examples of using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or water and lower alcohols. Examples include the use of a mixed solvent with (especially ethanol) or a mixed solvent of water and a polyhydric alcohol (especially 1,3-butylene glycol, glycerin). Among them, water alone or water with 1,3 -A mixed solvent of butylene glycol is particularly preferred.

  When the mixed solvent is used, for example, if the mixed solvent is water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1:10 to 20: 1, and the mixed solvent is water and ethanol. If it exists, it is preferable to set it as the range of 1: 10-20: 1 if it is a mixed solvent of 1: 10-25: 1 and water and glycerol.

  Further, the weight ratio of the dried portion of damask rose (for example, whole grass or flower) to the extraction solvent is preferably in the range of 1: 1 to 1:50, more preferably 1: 5 to 1:35. It is a range.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent. The extraction temperature is preferably in the range of 0 ° C. to 80 ° C., more preferably in the range of 0 ° C. to 20 ° C., and the extraction time is preferably 1 to 168 hours (1 hour to 1 week). More preferably, it is in the range of 1 to 120 hours (1 hour to 5 days).

  Prior to the extraction process of the present invention or in parallel with the extraction process, the damask rose may be hydrolyzed as necessary. As a result, the storage stability of the extract of damask rose can be improved and the extract as a cosmetic compounding agent can be used more effectively.

  In the case of subjecting the extract to an enzymatic hydrolysis treatment, examples of the enzyme include proteolytic enzymes such as actinase, papain and pepsin, starch degrading enzymes such as glucoamylase, α-amylase and β-amylase, cellulase, hemicellulase and pectinase. One or two or more selected from the group of enzymes of lipolytic enzymes such as fibrinolytic enzymes and lipases may be used, but one or more selected from these enzyme groups, respectively. It is more preferable to use a combination of these enzymes.

  The amount of the enzyme added is preferably in the range of 0.01 to 10% by weight, and more preferably 0. It is in the range of 1 to 2.0% by weight.

  In general, the extract prepared as described above may be adjusted to a pH of 3 to 8 and used as it is as a cosmetic compounding agent as it is or as a desired concentration by vacuum concentration or the like. You may do it. In addition, the extract may be dried by a conventional method such as spray drying.

  In addition, the extract prepared as described above may be refrigerated for a certain period of time in order to enhance storage stability and the like, and the supernatant may be used as a cosmetic compounding agent.

  The extract of the present invention obtained as described above contains various active ingredients. For example, as an organic acid by liquid chromatography (manufactured by Hitachi High-Technologies Corporation; LaChrom ELITE), It was confirmed that gallic acid, chlorogenic acid, and caffeic acid were contained.

  Examples of the use of the skin turnover improving agent containing the damask rose extract of the present invention include cosmetics (including quasi-drugs), for example, basic cosmetics such as emulsions, creams, lotions, essences, packs, lipsticks, Makeup cosmetics such as foundations, liquid foundations, makeup press powders, cheeks and white powders, facial cleansers, body shampoos, hair shampoos, soaps and other cleansing cosmetics, hair restorers, and bath preparations. However, of course, it is not limited to these.

  When the extract of damask rose of the present invention is used for cosmetics, the blending amount of the extract of damask roses is generally 0.002 to 1.0 weight in the case of basic cosmetics as the solid content of the extract. %, Preferably in the range of 0.02 to 0.2% by weight, in the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight, In the case of cleaning cosmetics, it is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight.

  When the extract of damask rose of the present invention is used in cosmetics, in addition to the essential damask rose extract, components usually used in cosmetics such as oily components, surfactants (synthetic, natural) Material), moisturizer, thickener, emulsifier or emulsifying aid, antiseptic / bactericidal agent, powder component, ultraviolet absorber, antioxidant, pigment, fragrance, other physiologically active ingredients, etc. as necessary Can be used together. Moreover, as long as the effectiveness and characteristics of the damask rose extract of the present invention are not impaired, there is no problem even if it is combined with other physiologically active ingredients.

  Here, as the oil component, for example, bergamot oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, Oils derived from plants such as avocado oil, macadamia nut oil, plant-derived squalane; Oils derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax , Hydrocarbons such as squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol; isopropyl myristate, isopropyl palmitate , Butyric oleate , 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Anions such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates Surfactant; quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers or emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

  Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components; silane root (white) extract; Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Plant-derived ethanol such as ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), propanediol, 1,2-pentanediol, various essential oils, dry bark, radish fermented liquor, sugarcane Alternatively, there is 1,3-butylene glycol.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powder, cereal (rice, wheat, corn, millet, etc.) powder, legume (soybean, red bean, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate) , Adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivative include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyltocopherylmaleic acid, ascorbyltocopherylphosphate K, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as prillyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L -L-ascorbic acid tetrafatty acid esters such as ascorbyl tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acyl thereof Derivatives, ascorbyl glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl phosphate L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives include tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxy. Cibenzoic acid and the like, nicotinic acid derivatives include, for example, nicotinic acid amide and benzyl nicotinate, and α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid, and the like. .

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, party extract or hydrolyzate thereof, pearl hydrolyzate, pearl seed fermented product, Royal jelly fermented product, sake lees extract or ceramide contained therein, sake lees fermented product, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. It is done. Coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (bergamot, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, apricot fruit extract, catamen giraffe, etc. Seaweed extract of sea urchin, extract of marine flowering plant such as sea cucumber, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, rice fermented extract, germinated rice extract or hydrolyzate thereof, germinated rice fermented Products, black bean extract or hydrolyzate thereof, bamboo shoot peel extract, linoleic acid and derivatives or processed products thereof (eg liposomal linoleic acid, etc.), animal or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, Glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichroic Acetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or fermented product thereof, red ginseng extract, beetroot extract, loofah extract, anaasa extract, peach extract , Peach extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, Paudarco bark extract, dairy lily extract or fermented product, hibiscus flower extract or fermented product, hagoromogusa extract, Cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, red rich extract, purple orchid extract, extract or hydrolyzate of yam peel or seed coat, safflower flower extract, casablanca extract, Sweet potato extract or fermented product thereof, guava leaf extract, dokudami extract, evening white potato extract, aloe extract , Fig flower extract, there is an apple extract or the like.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of damask rose flower extract solution After drying petals of Damascus rose belonging to the family Rosaceae, 225 g of purified water was added to 15 g of the dried product and immersed at 4 ° C., and then 225 g of 1,3-butylene glycol was added. . This was extracted at 4 ° C. and then filtered to obtain 387 g of a brown transparent damask rose flower extract solution (solid content concentration 1.01%).

Production Example 2 Preparation of extract of damask rose whole grass Damascus whole plant belonging to the genus Rosaceae is dried, and 225 g of purified water is added to 15 g of the dried product and immersed at 4 ° C., followed by 225 g of 1,3-butylene glycol. Added. This was extracted at 4 ° C. and then filtered to obtain 340 g of a brown transparent damask rose whole plant extract solution (solid content concentration 0.62%).

Formulation Example 1 Lotion [A component] part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Extract of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Amount that makes 100 parts in total [Component C]
Perfume Appropriate A component and B component were each heated to 80 ° C. or higher, then B component was added to A component and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 2 was used instead of the extract of Production Example 1 contained in the component B of Formulation Example 1.

Formulation Example 3 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Damask rose essential oil 0.025
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount of 100 parts [component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 4 Emulsion Emulsion in the same manner as in Formulation Example 3 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside in Formulation Example 3 Got.

Formulation Example 5 Emulsion In the same manner as in Formulation Example 3, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside, Obtained.

Formulation Example 6 Emulsion In the same manner as in Formulation Example 3, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 7 Emulsion In the same manner as in Formulation Example 3, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 8 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 1 2.5
Extract of Production Example 2 2.5
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 9 Lotion [Ingredient] part Extract of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Formulation Example 10 Essence [Ingredients] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid solution 0.1
Extract of Production Example 1 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water in an amount of 100 parts Hyaluronic acid was dissolved in purified water, and then the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Example 11 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Extract of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Formulation Example 12. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Extract of Production Example 2 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Formulation Example 13 Hair growth cosmetics [ingredients] Part Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-Menthol 0.8
Japanese cypress raft root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Gentian extract 2.0
Extract of Production Example 1 3.5
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine appropriate amount ethanol 20
The amount in which the total amount of purified water is 100 parts The above ingredients were sufficiently stirred and mixed to obtain a hair restorer.

Formulation Example 14. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Purified water Total amount of 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, stirring is continued and cooled to room temperature to obtain a hair shampoo It was.

Example 15. Hair conditioner [Component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[Component B] Part Extract of Production Example 2 2.0
1,3-butylene glycol 5.0
Purified water in an amount of 100 parts A component and B component were each heated to 80 ° C. and dissolved uniformly, then B component was added to A component, and stirring was continued to cool to room temperature to obtain a hair rinse. .

Test Example 1 Evaluation Test for Kallikrein-7 Activity Enhancement Effect In this Test Example 1, the activity of a chymotrypsin type serine protease (kallikrein-7) that promotes peeling of the epidermal horny layer and normalizes the turnover of the epidermal horny layer in normal skin. Enhancement was evaluated.
[Test method]
Normal human epidermal keratinocytes were prepared to 2 × 10 ⁵ cells / mL with HuMedia-KG2 [registered trademark] (produced by Kurabo Industries) containing a growth additive, and 100 μL was seeded in a 96-well microplate, and 5% carbonic acid was added. The cells were cultured at 37 ° C. under gas and saturated steam. After 24 hours, a culture solution containing the extract (sample solution) according to Production Example 1 of the present invention was added and cultured. Here, the sample solution was prepared so that the final concentration would be 0.5% and 1.0% as a solution with respect to the total amount of the culture solution. In addition, a test group to which a culture solution containing 50% 1,3-butylene glycol was added so that the final concentration was 1.0% was set as a control. After 72 hours, the cell culture supernatant in each test section was removed, and the cells were disrupted with 20 μL of Tris-HCl buffer (pH 8.0) containing 0.14 M sodium chloride and 0.1% Tween20. An enzyme solution was used. To the crude enzyme solution, add 30 μL of 100 mM Tris-HCl buffer (pH 7.8) containing 0.1% Tween 20 as a buffer and 50 μL of the above buffer containing 100 mM Suc-Leu-Leu-Val-Tyr-MCA as a substrate. The mixture was reacted at 37 ° C. for 1 hour with stirring. After completion of the reaction, fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems), and this was defined as kallikrein-7 activity. Further, another plate subjected to the same culturing operation was prepared, and after removing the culture supernatant, 100 μL of Hoechst 33342 solution was added and reacted at 37 ° C. for 1 hour. After the completion of the reaction, the fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader to obtain the amount of DNA. Using the obtained numerical value, the kallikrein-7 activity per DNA was calculated.

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, it was shown that the extract according to Production Example 1 of the present invention has an excellent activity-enhancing effect of kallikrein-7.

Claims (1)

A skin turnover ameliorating agent comprising a solvent extract of Rosa damascena, a plant of the Rosaceae (Rosaceae) genus (Rosa) as an active ingredient.