JP2015151388A - cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic which contains a component derived from a natural product excellent in biological safety, further exhibits remarkably excellent skin-beautifying effect and whitening effect, and is excellent in use feeling.SOLUTION: The present invention relates to an extract obtained from a guava, a plant of Myrtaceae Psidium, or a cosmetic containing the extract and collagen, the cosmetic being remarkably excellent in barrier function improving effect, moisturizing effect, antiaging effect, and whitening effect of a skin, and further excellent in use feeling when applying to the skin.

Description

  The present invention relates to a cosmetic compounding ingredient obtained from a plant belonging to the genus Bungiroceae, which has excellent skin physiological activity and biosafety, and a whitening and skin care cosmetic comprising such an ingredient.

  Skin aging is caused by internal factors such as inactivation of cell growth / differentiation associated with aging, decreased hormone secretion, quantitative decrease of extracellular matrix components, and cells caused by active oxygen induced by sunlight (ultraviolet rays). -Phenomenon caused by complex intertwining with external factors such as tissue damage or inflammation.

  Reactive oxygen, an external factor of skin aging, not only directly damages skin cells, but also denatures or crosslinks the collagen of the extracellular matrix component, resulting in wrinkle formation and reduced skin elasticity. Causes abnormal pigmentation and causes various damages to the skin, such as spots, freckles, and liver spots.

  In order to prevent this skin aging and keep the skin healthy and youthful, the use of various active ingredients has been proposed in the past, and cosmetics containing these active ingredients (beautifying skin ingredients, whitening ingredients, etc.) have been proposed. It is on the market. For example, vitamin C, vitamin E, superoxide dismutase (hereinafter abbreviated as SOD), an antioxidant such as catalase; an anti-inflammatory agent such as glycyrrhizic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract Cell activation components such as γ-amino-β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin and hyaluronic acid; and humectants such as urea. Further, arbutin, kojic acid, and the like are known as substances that suppress the occurrence of pigmentation such as skin spots, buckwheat, and melasma, and are widely used as active ingredients for whitening agents.

  However, conventional skin-beautifying agents and whitening agents have been proposed, but there are problems in terms of safety to the skin and the effectiveness when actually applied to the skin. Accordingly, there is a need for cosmetic ingredients with improved such points.

  In view of the problems of the prior art, the present inventors have intensively studied to find new skin-beautifying and whitening components derived from natural products from the viewpoint of skin safety. As a result, the extract of guava belonging to the genus Vanilla genus and the cosmetics containing the extract and collagen have excellent skin beautifying effects and whitening effects, and by combining these, skin safety and effectiveness are improved. We found that it would be possible to provide excellent cosmetics.

  Conventionally, cosmetics (Patent Documents 1 and 2) containing an extract of the guava belonging to the genus Bungiroceae as the active ingredient are known. However, the guava extract has a barrier function improving action and a moisturizing action. It has not been known that a combination of an extract and collagen can provide an excellent usability and whitening effect.

JP 05-246837 A Japanese Unexamined Patent Publication No. 07-188045

The present invention is a cosmetic containing an extract of guava belonging to the genus Vanilla.
The present invention is a cosmetic containing an extract of guava and collagen.
In the present specification, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

The present invention is a cosmetic comprising an extract of the genus Vanilla of the genus Pantheraceae, which is based on the skin barrier function improving action and moisturizing action exhibited by the extract, and has an excellent wrinkle and tarmi improvement, and skin. It is possible to provide a cosmetic that can improve the elasticity, gloss, and transparency. Further, the present invention is a cosmetic comprising a guava extract and collagen, further comprising collagen, thereby improving the feeling of use of the cosmetic, further improving the skin barrier function and moisturizing action, The effect of preventing and improving fine lines, spots, freckles, dullness, etc. can be enhanced.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The material used in the present invention may be any of guava whole plants, leaves, flower parts, stems, seeds, berries, roots, etc. belonging to the genus Vanilla genus, but the use of whole grasses or leaves is preferred.

  For the preparation of the extract, first, the guava part (for example, whole grass or leaf, flower part, stem, and / or fruit) of the genus Pantheraceae is washed with water if necessary to remove foreign substances, and then left as it is. Alternatively, after drying, it is chopped or crushed as necessary, and extracted by contacting with an extraction solvent. The extraction can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method, but a supercritical extraction method or a steam distillation method can also be used.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, water and lower alcohols are used in the present invention from the viewpoint of the effectiveness of the extract obtained, further from the viewpoint of skin irritation, and from the viewpoint that it can be widely applied to cosmetics. Or hydrophilic solvents such as polyhydric alcohols are preferred. Preferable examples of using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or water and lower alcohols. Examples include the use of a mixed solvent with (especially ethanol) or a mixed solvent of water and a polyhydric alcohol (especially 1,3-butylene glycol, glycerin). Among them, water alone or water with 1,3 -A mixed solvent of butylene glycol is particularly preferred.

  When the mixed solvent is used, for example, if the mixed solvent is water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1: 1 to 20: 1, and the mixed solvent is water and ethanol. If it exists, it is preferable to set it as the range of 1: 1 to 20: 1 if it is 1: 1 to 25: 1 and the mixed solvent of water and glycerol.

  Moreover, the weight ratio of the dry part (for example, whole grass or a leaf, a flower part, a stem, and / or a fruit) and the extraction solvent of the guava belonging to the genus Vanilla is preferably in the range of 1: 1 to 1:50. More preferably, it is in the range of 1: 5 to 1:20.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 4-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used, for example, extraction temperature and extraction time. For example, water or 1,3-butylene glycol, or water and 1,3 -When a mixed solution with butylene glycol is used as a solvent, the extraction temperature is preferably in the range of 0 ° C to 80 ° C, more preferably in the range of 0 ° C to 20 ° C, and the extraction time is preferably 1 hour. ~ 1 week, more preferably in the range of 4 hours to 3 days.

  Prior to the extraction process of the present invention or in parallel with the extraction process, the guava extract may be hydrolyzed as necessary. This may improve the storage stability of the guava extract and possibly more effectively use the extract as a cosmetic compounding agent.

  In the case of subjecting the extract to an enzymatic hydrolysis treatment, examples of the enzyme include proteolytic enzymes such as actinase, papain and pepsin, starch degrading enzymes such as glucoamylase, α-amylase and β-amylase, cellulase, hemicellulase and pectinase. One or two or more selected from the group of enzymes of lipolytic enzymes such as fibrinolytic enzymes and lipases may be used, but one or more selected from these enzyme groups, respectively. It is more preferable to use a combination of these enzymes.

  The amount of the enzyme added is, for example, 0.01 to 10% by weight in total with respect to the whole guava of the genus Vanilla genus or, if it is a leaf, flower part, stem and / or fruit, the solid content thereof It is preferable to set it as the range of this, More preferably, it is the range of 0.1 to 2.0 weight%.

  In general, the extract prepared as described above may be adjusted to a pH of 4 to 8 and used as it is as a cosmetic compounding agent as it is or as a desired concentration by vacuum concentration or the like. You may do it. In addition, the extract may be dried by a conventional method such as spray drying.

  In addition, the extract prepared as described above may be refrigerated for a certain period of time in order to enhance storage stability and the like, and the supernatant may be used as a cosmetic compounding agent.

  Next, the collagen or derivative thereof used in the present invention may be derived from animals or aquatic organisms. For example, collagen derived from horses, cattle, pigs, sheep, etc. if derived from animals, and aquatic organisms include salmon, tuna, flounder, shark, Thailand, kingfish, salamander, swordfish, scorpion, tilapia , Ray, bonito, eelfish, hammer, anago, eel, jellyfish and the like, but the present invention is not limited thereto. When aquatic organisms are used, there are no particular limitations on the site used for the extraction and collection of collagen, and skin, flesh, bones, folds, scales, and the like can be used.

Below, an example of the manufacturing method of the collagen of this invention or its derivative (s) is explained in full detail.
A known method can be used to extract collagen from the raw material. In order to increase the extraction efficiency of collagen from the raw material, it is preferable to chop, cut or pulverize the raw material by an appropriate means.

The collagen obtained as described above is useful as it is as a raw material for cosmetics and the like, but is further subjected to a succination treatment or the like to be chemically modified (for example, succinylated collagen), Alternatively, hydrolysis can be performed with an acid, alkali, enzyme, or the like, and a hydrolyzate (eg, hydrolyzed collagen, tripeptide, etc.) can be provided as a raw material for cosmetics.

  Examples of the collagen derivative by chemical modification include acylated derivatives such as succinylated collagen, phthalated collagen, maleylated collagen, and myristyl-succinylated collagen.

  The acylated derivative of collagen can be produced by reacting an acylating agent applied to collagen according to a conventionally known method for preparing acylated collagen.

As an example, a method for producing succinylated collagen is as follows.
First, an alkali such as sodium hydroxide or potassium hydroxide is added to an aqueous collagen solution to adjust the pH to 9 to 12 to insolubilize the collagen to obtain a collagen dispersion. As an acylating agent, 1 to 10 times (weight ratio) of succinic anhydride as an acylating agent is directly or dissolved in acetone, ethanol, methanol or the like, and gradually added while stirring at room temperature. During this time, the pH of the reaction solution is adjusted with an alkali such as sodium hydroxide or potassium hydroxide so that it does not fall below 9. When the insolubilized collagen is dissolved, the reaction is stopped, and an acid is added to the reaction solution to adjust the pH to 4-5. At this time, since the succinylated collagen is insolubilized and precipitated, it is recovered by filtration or centrifugation, and if necessary, succinylated collagen is obtained by washing and drying. This succinylated collagen is well soluble in neutral aqueous solutions of pH 5-8.

  The preparation of the collagen hydrolyzate of the present invention may be carried out in the same manner as the conventional method for preparing a collagen hydrolyzate. For example, the collagen obtained by the above process is treated with a proteolytic enzyme. The proteolytic enzyme is not particularly limited, and may be any of acidic protease, neutral protease, or alkaline protease.

The amount of enzyme added, the reaction time, and the treatment temperature can be set as appropriate. Usually, the amount of enzyme added is preferably 0.01 to 5% by mass relative to the solid content of the treatment liquid. The reaction time is preferably 0.5 to 6 hours, and more preferably 2 to 5 hours. Further, the reaction temperature is preferably 30 to 70 ° C, more preferably 40 to 60 ° C. In addition, after completion | finish of an enzyme reaction, it is preferable to inactivate an enzyme by heating.

Moreover, the collagen of the present invention may be subjected to a crosslinking treatment. For the crosslinking treatment, a conventional crosslinking treatment method can be used. For example, a treatment with a cross-linking agent (such as a peptide synthesis reagent), heat treatment, ultraviolet treatment, ozone treatment and the like can be mentioned.

  Examples of the guava extract of the present invention or cosmetics (including quasi-drugs) containing the extract and collagen include basic cosmetics such as emulsions, creams, lotions, essences, packs, lipsticks, foundations, and liquid foundations. Makeup cosmetics such as makeup press powder, blusher, white powder, cleansing cosmetics such as face wash, body shampoo, soap, hair shampoo, hair conditioner, hair care cosmetics such as hair restorer, and bath preparations Of course, it is not limited to these.

  The blending amount of the guava belonging to the genus Vanilla genus in the cosmetics of the present invention is generally 0.002 to 1.0% by weight, preferably 0.02 in the case of basic cosmetics, as the solid content of the extract. In the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. In general, it is in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight. Moreover, in the case of hair care cosmetics, the solid content of the extract is generally 0.00001 to 5.0 wt% (solid content wt%, the same shall apply hereinafter), preferably 0.0001 to 3 0.0% by weight.

  In the cosmetics of the present invention, in addition to the essential component guava extract, or the extract and collagen, components commonly used in cosmetics, such as oily components, surfactants (synthetic systems, natural products systems), Moisturizers, thickeners, emulsifiers or emulsifying aids, antiseptic / bactericidal agents, powder components, ultraviolet absorbers, antioxidants, pigments, fragrances, other physiologically active ingredients, etc. may be appropriately blended as necessary. it can. In addition, as long as the effectiveness and features of the guava extract of the present invention are not impaired, it may be combined with other physiologically active ingredients in cosmetics.

Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

As emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and derivatives thereof, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.) and the like can also be blended.

Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) extract, various amino acids And their derivatives.

Examples of the thickener include components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan and fucoidan; silane root (white) extract; polysaccharides such as pectin, locust bean gum and aloe polysaccharide; xanthan gum, Gums such as tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; Examples include glutamic acid and its derivatives.

Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate, etc.), peony extract, silane root (white) extract, etc. There is.

Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhodenol, 4- (4-hydroxyphenyl) -4-butanol)), hydroxybenzoic acid And derivatives thereof, vitamin E and derivatives thereof, α-hydroxy acid, and AMP (adenosine monophosphate, adenosine monophosphate). These may be used alone or in combination.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, ascorbic acid sugar derivatives such as L-ascorbic acid-5-glucoside, 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is Hexanoyl group, o L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid- 2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acylated derivatives thereof, ascorbyl glycerol derivatives such as bisglyceryl ascorbic acid, as hydroquinone derivatives, arbutin (hydroquinone-β-D-glucopyranoside), α-Arbutin (hydroquinone-α-D-glucopyranoside) and the like as tranexamic acid derivatives include tranexamic acid esters (for example, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranet And amides of tranexamic acid (for example, tranexamic acid methylamide). Resorcinol derivatives include, for example, 4-n-butylresorcinol, 4-isoamylresorcinol, and the like. Examples of the -dihydroxybenzoic acid derivative include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic acid derivative include nicotinic acid amide. Examples of the α-hydroxy acid such as benzyl nicotinate include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

Examples of physiologically active ingredients include whitening ingredients such as placenta extract, Sakuhakuhi extract, Yukinoshita extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peonies Extract or its hydrolyzate, lactic acid bacteria fermented rice, damask rose extract, apricot extract, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, banquet extract, pearl barley hydrolysate , Barley seed fermented product, royal jelly fermented product, sake lees fermented product, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. As an aging component, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (water eggplant, long eggplant, Kamo eggplant, rice Etc.) Extracts or hydrolysates thereof, extracts of seaweeds such as catamen giraffes, extracts of marine flowering plants such as sea eels, fermented soymilk, jellyfish water, rice extract or hydrolysates thereof, rice fermented extracts Germinated rice extract or hydrolyzate thereof, fermented germinated rice, black bean extract or hydrolyzate thereof, linoleic acid and derivatives or processed products thereof (eg liposomal linoleic acid), elastin and derivatives thereof, glycyrrhizic acid and Derivatives thereof (dipotassium salts, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, aloe extract , Honeycomb extract, Loofah extract, Anaaaosa extract, Zizyphus joazei ro) There are extracts, etc.

Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of guava leaf extract The guava leaf was dried, 225 g of purified water was added to 10 g of the dried product and immersed at 4 ° C., and then 225 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered to obtain 360 g of a brown transparent guava leaf extract solution (solid content concentration 0.50%).

Production Example 2 Preparation of Guava Whole Grass Extract The whole guava grass was dried, 225 g of purified water was added to 10 g of the dried product and immersed at 4 ° C., and then 225 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered to obtain 330 g of a brown transparent guava leaf extract solution (solid content concentration 0.45%).

Production Example 3 Preparation of Halo Collagen 30 g of frozen Peel (Muraenesox cinereus) skin was thawed and cut into 10-20 mm squares. The operation of adding 900 mL of purified water to the shredded product, homogenizing with a juicer mixer, and discarding the liquid phase part was repeated three times to sufficiently wash the fish skin shredded product with water. Next, the washed fish skin was sufficiently drained, 900 mL of 0.5 M acetic acid aqueous solution containing 0.03 g of pepsin was added thereto, and the mixture was stirred at 4 to 10 ° C. for 24 hours to extract collagen. After filtering this extract to remove the fish skin of the extraction residue, sodium chloride was added to the resulting clarified extract to 1 M with stirring, and stirring was continued for another hour after the addition was completed. Was precipitated. The precipitated collagen was recovered by centrifugation, suspended in 900 mL of 50 mM Na ₂ HPO ₄ solution, adjusted to pH 7.5 and stirred for 24 hours, and then centrifuged again to recover collagen. Next, the operation of suspending the recovered collagen in 900 mL of purified water, washing with stirring and centrifuging is repeated three times, and then the obtained purified collagen is dissolved in a 0.02 wt% aqueous phosphoric acid solution to obtain a solid content. 450 g of a substantially colorless collagen aqueous solution with a concentration of 0.3% by weight was obtained.

Production Example 4 Preparation of hemocollagen (hydrolysis)
Purified collagen recovered by the same process as in Production Example 3 was dispersed in purified water so as to be a 1.0 wt% suspension, and then heated to 40 ° C. 0.015 g of proteolytic enzyme was added, hydrolyzed at 50 ° C. for 2 hours, and then heated at 80 ° C. for 1 hour to inactivate the enzyme. Filtration purification was performed to obtain 140 g of a slightly yellow transparent collagen hydrolyzate having a solid concentration of 0.9% by weight.

Production Example 5 Preparation of collagen (succinylation)
30 g of frozen skins of peach (Muraenesox cinereus) were thawed and cut into 10 to 20 mm squares. The operation of adding 900 mL of purified water to the shredded product, homogenizing with a juicer mixer, and discarding the liquid phase part was repeated three times to sufficiently wash the fish skin shredded product with water. Next, the washed fish skin was sufficiently drained, 900 mL of 0.5 M acetic acid aqueous solution containing 0.03 g of pepsin was added thereto, and the mixture was stirred at 4 to 10 ° C. for 24 hours to extract collagen. The extract was filtered to remove the fish residue of the extraction residue, and then the clarified extract obtained was adjusted to pH 9.5 by adding 10 wt% NaOH aqueous solution. The succinic anhydride 1.5g was added to this, and it stirred at 4-10 degreeC for 24 hours. During this time, the pH of the reaction solution was adjusted with a 10 wt% NaOH aqueous solution so that it did not fall below 9. After completion of the reaction, citric acid was added to adjust the pH to 4. Insolubilized collagen was collected by centrifugation. The collected collagen was dispersed in a phosphate buffer (pH 6.5), stirred for 1 hour, and then centrifuged again to collect collagen. Next, the operation of suspending the recovered collagen in 900 mL of purified water, washing with stirring and centrifuging is repeated three times. The obtained purified succinylated collagen is suspended in purified water, and 0.5 M NaOH is added. The solution was dissolved by adjusting the pH to 6.5. 300 g of a substantially colorless collagen aqueous solution with a solid content concentration of 0.3% by weight was obtained.

Production Example 6 Preparation of eel collagen 350 g of an approximately colorless collagen aqueous solution with a solid content concentration of 0.3% by weight was obtained in the same manner as in Production Example 3, except that eel (Anguilla japonica) was used instead of the peel.

Production Example 7 Preparation of Maanago Collagen In the same manner as in Production Example 3 except that the skin of maago (Conger myriaster) was used in place of the peel, 300 g of a substantially colorless collagen aqueous solution was obtained at a concentration of 0.3% by weight.

For example, the cosmetic composition of the present invention is obtained by mixing the guava extract of Production Example 1 or 2 obtained above and any one or more collagens of Production Examples 3 to 7.

The blending ratio of the guava extract and the collagen is preferably 1: 10000 to 500: 3, more preferably 1: 2000 to 1000: 0.1 in terms of the weight ratio of the solid content.

Formulation Example 1 Lotion [A component] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[Component B] Extract of part production example 1 1.0
Collagen of Production Example 3 1.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide appropriate amount Purified water Amount that makes the total amount 100 parts [component C]
Fragrance Appropriate amounts of component A and component B were each heated to 80 ° C. or higher, component B was added to component A and stirred, and then homogenized for 2 minutes with Hiscotron (5000 rpm). After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation Example 2 A lotion was obtained in the same manner as in Formulation Example 1, except that 1.0 part of the extract of Production Example 2 was used instead of the extract of Production Example 1 contained in the B component of Formulation 1.

Formulation Example 3 A lotion was obtained in the same manner as in Formulation Example 1, except that the collagen of Production Example 4 was used instead of the collagen of Production Example 3 contained in the B component of Formulation 1.

Formulation Example 4 A lotion was obtained in the same manner as in Formulation Example 1 except that the collagen of Production Example 5 was used instead of the collagen of Production Example 3 contained in the component B of Lotion Formulation 1.

Formulation Example 5 A lotion was obtained in the same manner as in Formulation Example 1, except that the collagen of Production Example 6 was used instead of the collagen of Production Example 3 contained in the B component of Formulation 1.

Formulation Example 6 A lotion was obtained in the same manner as in Formulation Example 1, except that the collagen of Production Example 7 was used instead of the collagen of Production Example 3 contained in the B component of Formulation 1.

Formulation Example 7 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[Component B] Part Extract of Production Example 1 1.0
Collagen of Production Example 3 1.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 8 Emulsion In the same manner as in Formulation Example 8, except that 2.0 parts of tranexamic acid is used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the B component of Formulation Example 7. Got.

Formulation Example 9 Emulsion In the B component of Formulation Example 7, the emulsion was prepared in the same manner as Formulation Example 5 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Formulation Example 10 Emulsion In the same manner as in Formulation Example 5, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 11 Emulsion In the component B of Formulation Example 7, an emulsion was obtained in the same manner as Formulation Example 5 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide were used instead of dipotassium glycyrrhizinate. It was.

Formulation Example 12. Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[Component B] Part Extract of Production Example 1 1.0
Collagen of Production Example 3 1.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 13 Lotion [Ingredient] Part Extraction Example 2 Extract 1.0
Collagen of Production Example 4 1.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Fragrance Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts.

Formulation Example 14. Essence [ingredient] part ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Extract of Production Example 1 1.0
Collagen of Production Example 3 1.0
Citric acid 0.3
Sodium citrate 0.6
Purified water After hyaluronic acid was dissolved in purified water in an amount of 100 parts, the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Example 15. Liquid foundation [component A] part stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[Component B] Extract of part production example 1 1.0
Collagen of Production Example 3 1.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Total amount of 100 parts [component C] parts Titanium oxide 8.0
Talc 4.0
Coloring pigment Appropriate amounts The above-mentioned components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Formulation Example 13 Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[Component B] Part Extract of Production Example 2 1.0
Collagen of Production Example 5 1.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Formulation Example 14. Shampoo for hair care [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
Collagen of Production Example 3 1.0
1,3-butylene glycol 2.0
Purified water Amounts of 100 parts in total A and B components are each heated to 80 ° C to dissolve uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a hair shampoo It was.

Comparative Example 1 Lotion [A component] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[Component B] Extract of part production example 1 1.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide appropriate amount Purified water Amount that makes the total amount 100 parts [component C]
Perfume

Comparative Example 2 Lotion Toner lotion was obtained in the same manner as in Comparative Example 1, except that 1.0 part of collagen of Production Example 3 was used instead of Extract 1.0 of Production Example 1 in Component B of Comparative Example 1.

Test Example 1 Method for measuring stratum corneum moisture content [Sample preparation]
The extract of Production Example 1 or 2 according to the present invention, and the extract of Production Example 1 or 2 and the collagen of any of Production Examples 3 to 7 were mixed, and the composition of the present invention was prepared as follows.
[Table 1]

[Test method] Method for measuring stratum corneum moisture content A test part (15 mm x 15 mm) was set on the inner side of the left forearm of the test subject. The stratum corneum water content of each test part was measured 5 times using Skicon-200, and the average was taken as the initial value of each test part. After measuring each initial value, set up 7 test plots and control plots, apply lotion containing 1.0% each of samples 1 to 7 of this invention (apply to test plots), and purify instead of samples Application of a lotion containing 2% of water (application to the control area) was started (twice a day). Two weeks later, the stratum corneum water content of each test part was measured in the same manner. As the test result, the amount of change in the stratum corneum water content from the initial value of each test part was determined. The test results were expressed as relative values with a control value of 100.

The results of Test Example 1 are shown in Table 2.
[Table 2]

As shown in Table 2, the guava extract according to the present invention and the composition containing the extract and collagen remarkably improve the retention of the skin stratum corneum moisture content. Can be improved, and the transparency of the skin can be enhanced.

Test Example 2 Monitor test (feeling of use)
About the lotion (formulation example 1) and the comparative example 1 which mix | blended the composition of this invention, the usability | use_condition was evaluated by the actual use test by a monitor.

[sample]
(1) Lotion of Formulation Example 1 (Invention Example 1)
(2) Emulsion of Comparative Formulation Example 1 (Comparative Example 1)

[Test method]
Twenty randomly selected women aged 18 to 50 years were used as subjects, and each subject received the skin lotion according to Invention Example 1 and the skin lotion according to Comparative Example 1 twice a day (morning in the morning). , Evening) The following 4 items (I to D) were evaluated for the feeling of use and skin condition when applied for one month. Each evaluation was performed according to a five-step evaluation of A: very good, B: good, C: normal, D: somewhat bad, and E: bad.
(Feeling of use)
A. The feel of the hand
B. Elongation during application c. Smoothness when applied. Feel after application

The results of Test Example 2 are shown in Table 3.
[Table 3]

As shown in Table 3, it has been clarified that the combined composition of guava extract and collagen according to the present invention has an excellent usability.

Test Example 3 Monitor test (skin quality improvement)
About the lotion (formulation example 1) and the comparative examples 1 and 2 which mix | blended the composition of this invention, the skin quality improvement effect (stain, buckwheat, dullness) was evaluated by the actual use test by a monitor.

[sample]
(1) Lotion of Formulation Example 1 (Invention Example 1)
(2) Lotion of Comparative Formulation Example 1 (Comparative Example 1)
(3) Lotion of Comparative Formulation Example 2 (Comparative Example 2)

[Test method]
Randomly extracted 40 women aged 18-50 were divided into two groups (A, B) of 20 subjects as subjects, and each group was compared with Invention Example 1 and Comparative Example 1 or Invention Example 1. One of the two types of lotion combinations in Example 2 was allocated, and each of the left and right cheeks was applied with the emulsion of the present invention or the comparative example twice a day (morning and evening) for one month. The condition of the skin at the time was evaluated for the following three items (i to ha). Each evaluation was performed according to a five-step evaluation of skin quality improvement effects: A: improved, B: slightly improved, C: unchanged, D: slightly worsened, E: worsened. It was.
(Skin quality)
A. Simi, Sobacus b. Dull

The results of Test Example 3 are shown in Table 4.
[Table 4]

As shown in Table 4, it is clear that the composition according to the present invention containing the guava extract and collagen according to the present invention has an excellent skin quality improving effect (improved spots, buckwheat, dullness). became.

Claims (2)

A cosmetic comprising an extract of guava which is a plant of the Myrtaceae family (Psidium) as an active ingredient and having an action of improving skin barrier function and a moisturizing action. A cosmetic comprising an extract of guava which is a plant of the Myrtaceae family Psidium and collagen.