JPH08151315A - Skin cosmetic - Google Patents

Abstract

PURPOSE: To obtain a skin cosmetic excellent in aged skin-improving effects such as a rough skin-improving effect, a corneum-improving effect, a blood circulation-stimulating effect, and a skin-beautifying effect by compounding a kind of glucoprotein, kallidinogenase. CONSTITUTION: The skin cosmetic contains kallidinogenase which is a glucoprotein having a mol.wt. of approximately 30000. The kallidinogenase exhibits pharmacological actions such as a telangiectatic action, a blood flow- increasing action and a tissue metabolism-improving action. The skin cosmetic stimulates the blood circulation in the skin to improve the functions of the skin, repairs or improves the original functions of the skin to hold the skin in a healthy state, and especially exhibits the remarkable effects, when applied to aged skin. The kallidinogenase is preferably compounded in an amount of 0.001-5.0wt.% based on the whole amount of the skin cosmetic. The skin cosmetic can be applied to e.g. lotions, milky lotions, creams, or packs.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin cosmetic having excellent aged skin improving effects (rough skin improving effect, keratin improving effect, blood circulation promoting effect, beautiful skin effect, etc.).

[0002]

Aging skin is dry and non-smooth rough skin in which keratinocyte exfoliation is observed, and the connective tissue has a high collagen / elastin ratio and wrinkles. Also, in aging skin, the turnover of the stratum corneum is slow due to a decrease in cell metabolism, and therefore, it is said that the turnover is accelerated when the antiaging effect is imparted to the skin, and various turnover components of skin tissue are activated. Is being studied.

As a cosmetic having an effect of preventing skin aging, a cosmetic containing vinpocetine or the like has been proposed up to now (Japanese Patent Laid-Open No. 5-194179), but in practice, it has a function of skin tissue. It has been difficult to obtain a dermocosmetic which is repaired or improved, and the function originally possessed by the skin is restored to improve the antiaging effect of the skin.

Therefore, as a result of intensive studies in view of the above circumstances, the present inventor has found that the skin cosmetics described below are excellent in the aged skin improving effect (rough skin improving effect, keratin improving effect, blood circulation promoting effect, beautiful skin effect, etc.). After confirmation, the present invention was completed.

[0005]

That is, the object of the present invention is to provide a skin cosmetic excellent in the effect of preventing skin aging (rough skin improving effect, keratin improving effect, blood circulation promoting effect, beautiful skin effect, etc.). It is in.

[0006]

The above-mentioned object is achieved by incorporating calidinogenase into skin cosmetics.

The kalidinogenase used in the present invention is a known substance, and its pharmacological effects include dilation of peripheral blood vessels, blood flow increasing action, and tissue metabolism improving action, and it is applied to the improvement of hypertension and the like. ing. Calizinogenase is a glycoprotein with a molecular weight of about 30,000.

[0008] A skin cosmetic composition containing the calidinogenase of the present invention promotes blood circulation in the skin to enhance skin function, and restores or improves the function originally possessed by the skin to make the skin healthy. When applied to the skin, especially when applied to aged skin, a remarkable effect is observed.

The amount of the above-mentioned kalidinogenase is preferably 0.001 to 5.0% by weight based on the total amount of skin cosmetics.

The skin cosmetic of the present invention can be applied to lotions, emulsions, creams, packs and the like.

In addition to the above, the skin cosmetic of the present invention may be appropriately blended with a dye, a fragrance, an antiseptic, a surfactant, a pigment, an antioxidant, etc. within a range that achieves the object of the present invention. it can.

[0012]

EXAMPLES The present invention will be described in detail below based on examples and comparative examples.

Examples 1-8, Comparative Examples 1-2

The test method used in the present invention is as follows.

[Rough Skin Improvement Effect Test] The effect of continuous application for 4 weeks was examined for 10 middle-aged and elderly subjects having rough skin on the lower leg. 1 day per day on the left lower leg outer test site of the subject
About 0.5 g of the sample was applied, and the condition of the skin before and after the start of the test was evaluated according to the criteria shown in Table 1. The lower right leg was not coated with the sample and served as a control.

[0016]

[Table 1]

The determination results of the test site before and after the test and the control site are compared, and when the skin dryness is improved by two stages or more (for example, + →-, ++ → ±), the case where it is improved by one stage is slightly effective. Valid, invalid when there was no change. The test results were shown by the number of subjects who were valid or slightly valid.

[Test for effect of improving keratin (increasing anti-peeling property of keratinocytes)] Scotch tape (Nichiban Mendeing Tape) was adhered to the skin of the test area before and after the start of the above-described test for improving rough skin effect, and this The state of keratinocytes attached to the tape when peeled off was examined in detail by a scanning electron microscope, and the skin keratinocyte anti-peeling property was analyzed according to the criteria in Table 2 to determine the keratin improving effect.

[0019]

[Table 2]

The evaluation was made effective when the difference between the evaluation points of the test site and the control site after continuous application for 4 weeks was improved by 2 points or more, 1 case was slightly effective, and 0 point was not effective. . The judgment results are shown by the number of subjects who were valid and slightly valid.

[Skin blood flow test method] The abdomen of three New Zealand White rabbits was shaved and fasted for 18 hours, and then pentoparbitol sodium salt was intravenously injected at a rate of 35 mg / kg. Then, anesthesia was performed. Next, a plate type transducer was fixed on the sample application site (test site) on the abdomen with cellophane tape, and the cross thermoelectric skin flow rate (Shinei Co., Ltd., model 201). Was used to measure the skin blood flow (μV). The sample was uniformly applied to a 3 × 2 cm skin site by 0.1 g, and the blood flow rate (CB) before the sample application and a certain time after the sample application (for example, 0.
Blood flow rate (Ct) after 5, 1.0, 2.0 hours) was measured, and the blood flow rate of increase (%) was calculated by the following formula. The test result was shown by the average value of the blood flow rate increase rate of 3 birds. Blood flow (%) = [(Ct-CB) / CB] x 100

[Sensory test (Beautiful skin effect test)] Rough skin,
Female subject (35-55 years old) who complains of fine wrinkles, dry skin, etc.
The sample was applied to 10 persons twice a day (morning and evening) for 4 consecutive weeks, and the effect after 4 weeks was evaluated. The test results were expressed by the number of people who answered that the skin was moistened, the skin became smooth, and the skin became taut for each item of skin wettability, smoothness, and elasticity.

Examples 1 to 4 and Comparative Example 1

[Skin Lotion] Each skin lotion was prepared by blending the composition shown in Table 3 with calidinogenase as shown in Table 4 by the following preparation method.

[0025]

[Table 3]

(Preparation Method) The C component of calidinogenase is B
After being mixed with the components to uniformly dissolve the components A and B, the components A and B were mixed and dispersed by stirring, and then filled in a container.
At the time of use, the content was uniformly dispersed by shaking before use.

The results of carrying out the various tests are shown in Table 4.

[0028]

[Table 4]

As shown in Table 4, the skin lotions containing the calidinogenases of Examples 1 to 4 showed good results in various tests as compared with Comparative Example 1.

Examples 5 to 8 and Comparative Example 2

[Skin Cream] Each skin cream was prepared by blending the composition shown in Table 5 with calidinogenase as shown in Table 4 by the following preparation method.

[0032]

[Table 5]

(Preparation Method) The C component of calidinogenase is B
Each of the skin creams was prepared by blending the ingredients with each other, heating and dissolving each of the components A and B at 80 ° C., mixing and stirring and cooling to 30 ° C.

The results of the above tests are shown in Table 4 above.

As shown in Table 4, it is clear that the skin creams of Examples 5 to 8, which are the skin cosmetics of the present invention, are superior in all of their characteristics as compared with the respective comparative examples. There were no abnormalities in the compounding characteristics.

[0036]

Industrial Applicability As described above, the present invention can provide a skin cosmetic excellent in the skin aging prevention effect (rough skin improving effect, keratin improving effect, blood circulation promoting effect, beautiful skin effect, etc.).

Claims (1)

[Claims] 1. A skin cosmetic comprising a mixture of calidinogenase.