KR20180037728A - Cosmetic composition containing the mixed extract of pomegranate, rosa centifolia flower, carthamus tinctorius and rosa rugosa - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of pomegranate, cabbage rose, safflower, and sweetbrier as an active ingredient. To this end, the content of the mixed extract composed of pomegranate, cabbage rose, safflower, and sweetbrier is 0.0001-30.0 wt% as the active ingredient with respect to the total weight of the cosmetic composition. According to the present invention, the mixed extract composed of pomegranate, cabbage rose, safflower, and sweetbrier has inhibitory effects on matrix metalloproteinase-1 (MMP-1) production, collagen biosynthesis effects, antioxidative effects, skin wrinkle improvement effects, skin elasticity improvement effects, and moisturizing effects, thereby enabling production of various functional cosmetic products.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a mixed extract of pomegranate, pomegranate, rose flower, safflower and garlic.

The present invention relates to a cosmetic composition containing a mixed extract of pomegranate, western rose flower, safflower, and marigold.

A variety of physical and chemical changes occur in human skin during the aging process. The cause of this phenomenon is divided into intrinsic aging and photo-aging. Free radicals can be caused by activation of ultraviolet rays, stress, disease state, environmental factors, wounds, and aging. When such state is deepened, the antioxidant defense network existing in the living body is destroyed, And aging.

More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major components of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins can cause collagen (collagen), hyaluronic acid, elastin, proteoglycan, fibronectin, and the like that are severely hyperinflammatory reactions and skin elasticity If this gets worse, DNA mutations can lead to mutations, cancer, and immune deficiency.

Therefore, it is necessary to protect the cell membrane by destroying the free radicals mediated by free radicals, ultraviolet rays, and inflammation that occur during the metabolism of the body, and to regenerate already damaged cells by active metabolism, Can quickly recover and maintain healthy skin.

Aging involves not only these free radicals, but also enzyme called matrix metalloproteinase (MMP), a collagenase. That is, synthesis and degradation of extracellular matrix such as collagen in vivo are appropriately controlled, but collagen synthesis is reduced as aging progresses, and expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And the wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation.

Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity. Until now, the raw materials used as cosmetic materials mostly inhibited only the enzyme activity. Therefore, we tried to control the amount and activity of MMP expression induced by intracellular natural aging and photoaging.

Korean Patent Laid-Open Publication No. 2016-0087113 discloses cosmetics including various flowers such as marigold flower, camellia flower, apricot flower, cucumber flower, peony flower, rose, azalea, and Japanese hiragana. Korean Patent Publication No. 2011-0001538 discloses a cosmetic composition having antioxidative and whitening activity, which includes various flowers such as lily of the valley, bamboo flower, magnolia flower, and acacia flower.

The inventors of the present invention have studied the applicability of various natural products to cosmetics. As a result, they have found that pomegranate, western rose flower, safflower and safflower are selected and their extracts are used to produce MMP-1 inhibitory effect, collagen biosynthesis Antioxidative effect, and moisturizing effect of the present invention. As a result, it was found that the efficacy as a cosmetic product can be expected.

It is an object of the present invention to provide a cosmetic composition which contains MMP-1 production inhibitory effect, collagen biosynthesis effect, antioxidative effect and moisturizing effect by containing a mixed extract of pomegranate, western rose flower,

It is also an object of the present invention to provide a cosmetic composition that exhibits an efficacy by preparing and purifying supercritical and ultrasound to maximize the efficacy of a mixed extract of pomegranate, western rose flower, safflower, and Korean poppy.

It is another object of the present invention to provide a cosmetic method using a cosmetic composition containing a mixed extract of pomegranate, western rose flower, safflower, and common flower.

The present invention relates to a cosmetic composition containing, as an active ingredient, a mixed extract of pomegranate, western rose flower, safflower, and green tea.

Preferably, the cosmetic composition is for improving skin wrinkles.

Preferably, the cosmetic composition is for antioxidation.

Preferably, the cosmetic composition is for moisturizing.

Preferably, the cosmetic composition is for improving skin elasticity.

Preferably, the mixed extract of pomegranate, western rose flower, safflower, and marigold is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the mixed extract of pomegranate, western rose flower, safflower and safflower is selected from the group consisting of (a) water, anhydrous or lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, (B) a supercritical extraction method or (c) an ultrasonic extraction method using an extraction solvent selected from the group consisting of acetonitrile, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof.

The combined extracts of pomegranate, western rose flower, safflower, and hornblende according to the present invention exhibited an effect of inhibiting MMP-1 production (Experimental Example 1), enhancing collagen synthesis (Experimental Example 2), antioxidative effect (Experimental Example 3) Experimental Example 4).

Further, according to the present invention, in order to maximize the efficacy of the mixed extracts of pomegranate, western rose flower, safflower and Korean blended flowers, the effect of inhibiting MMP-1 production and enhancing collagen synthesis are further enhanced through ultrasonic extraction or supercritical extraction .

The cosmetic composition of the present invention contains, as an active ingredient, a mixed extract of pomegranate, western rose flower, safflower, and common flower.

Pomegranate has been widely used to make beverages and ice creams from ancient Solomon kingdom, and pomegranate flowers and the bark of less ripe fruit have been used as raw materials for red dyes. The flowers are reddish, yellow, white, and various colors. When they are ripe, the fruit is opened in a round shape. It contains a large amount of vegetable estrogen and is called a female fruit.

The western rose flower is a rose flower that emits a sweet and fantastic scent, and Greek and Roman doctors used it as a tonic. In China, it is said that roses have a function of bloodshed and bloodshed in oriental medical aspect, and they have been used as a beauty material for skin care by massaging rose powder after bathing.

Safflower, also known as safflower, has been widely used as a medicine to relieve ejaculation from traditional medicine in traditional medicine and has been used for women's diseases, bronchitis and abdominal pain.

It is a plant that is loved more than the roses, because it smells of deciduous shrubs in the sandy beaches and mountain bottoms of each place in Korea. The petals are used for food preparation and fatigue recovery. The fruit is a natural antioxidant rich in vitamin C, and the root has a variety of uses, including the role of liver protection and the use of juvenile sterling silver.

The mixed extract of pomegranate, western rose flower, safflower, and rhizome of the present invention can be extracted by mixing the same amount of pomegranate, western rose flower, safflower and japonica or by mixing them in different amounts.

The mixed extract of pomegranate, western rose flower, safflower and marigold of the present invention can be prepared according to a conventional method known in the art, that is, under the conditions of ordinary temperature and pressure, using a conventional solvent, (A) water, anhydrous or lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, Solvent extraction method using a solvent selected from the group consisting of ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and mixtures thereof (b) supercritical extraction with carbon dioxide and supercritical extraction with high temperature or (c) .

In the present invention, the mixed extracts of pomegranate, western rose flower, safflower and safflower are mixed with various extraction solvents, for example, water, anhydrous or hydrated lower alcohol (for example, methanol, ethanol, Is obtained using at least one extraction solvent selected from the group consisting of propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and mixtures thereof , Preferably obtained using ethanol, 70% (v / v) ethanol or water, and most preferably 70% (v / v) ethanol.

On the other hand, it is obvious to those skilled in the art that the mixed extract of pomegranate, western rose flower, safflower and marigold of the present invention can obtain an extract having substantially the same effect not only by the above-mentioned extraction solvent but also by other extraction solvent .

In addition, the mixed extract of pomegranate, western rose flower, safflower and marigold of the present invention includes not only the extract obtained by the above-mentioned extraction solvent, but also the extract obtained through ordinary purification and fermentation process. For example, decompression by carbon dioxide, extraction by supercritical extraction with high temperature, extraction by extraction using ultrasound, separation by ultrafiltration membrane with constant molecular weight cutoff value, various chromatography (size, charge, hydrophobic or affinity And the active fraction obtained through various purification and extraction methods, such as an extract obtained by fermentation products using natural or various microorganisms, are also included in the extract of the present invention.

Further, the present invention provides a cosmetic composition characterized in that the above extract is obtained by liquid-freezing obtained by cold-pressing and heating filtration at room temperature, and further by concentrating or lyophilizing the solvent under reduced pressure.

Supercritical fluid extraction refers to supercritical fluid extraction. In general, supercritical fluids are extracted from liquids and gases when the gas reaches a critical point under high-temperature and high-pressure conditions. And has chemically similar polarity to non-polar solvents. Due to these properties, supercritical fluids have been used to extract lipophilic substances (J. Chromatogr. A. 1998; 479: 200-205). Carbon dioxide is a supercritical fluid with both liquid and gaseous nature, with the operation of supercritical fluid equipment reaching its critical pressure and temperature, resulting in increased solubility in fat-soluble solutes. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide. When the supernatant carbon dioxide containing a small amount of cosolvent is passed through the sample remaining in the extraction vessel after extracting the lipid-soluble substance, components that were not extracted by pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a mixed fluid in which a cosolvent is further mixed with supercritical carbon dioxide or carbon dioxide. These co-solvents may be used alone or in admixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether. Most of the extracted samples contain carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed by a reduced pressure evaporator.

Ultrasonic extraction is an extraction method using energy generated by ultrasonic vibration. Ultrasonic waves can destroy insoluble solvent contained in a sample in a water-soluble solvent. Due to the high local temperature generated at this time, Since the kinetic energy is increased, sufficient energy required for the reaction is obtained, and the high pressure is induced by the impact effect of the ultrasonic energy, thereby increasing the mixing efficiency of the substance contained in the sample and the solvent, thereby increasing the extraction efficiency. As the extraction solvent which can be used for the ultrasonic extraction method, one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether can be used. The extracted sample is recovered by vacuum filtration, and the filtrate is recovered, and the extract is removed by a vacuum evaporator and freeze-dried to obtain an extract.

The mixed extract of pomegranate, western rose flower, safflower, and rhizome according to the present invention is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition, more preferably, the pomegranate, rose flower, safflower, Is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition. When the content of the extract is less than 0.0001% by weight, the effect of improving the skin is not exhibited. When the content of the extract is more than 30.0% by weight, the degree of improvement of the skin against the increase of the content is insignificant, It is not even.

The cosmetic composition of the present invention comprising the pomegranate, western rose flower, safflower and mixed extract of pomegranate having various functions as described above is excellent in collagen synthesis promoting effect, MMP-1 formation inhibiting effect, antioxidant effect, skin wrinkle improving effect, moisturizing effect .

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, and the surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

On the other hand, the cosmetic process of the present invention refers to all cosmetic processes using the cosmetic composition of the present invention. That is, all methods known in the art using a cosmetic composition belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. The cosmetic composition according to the present invention may be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

The cosmetic method using the cosmetic composition comprising the pomegranate, rosacea, safflower and safflower mixture of the present invention can provide an effect of inhibiting MMP-1 production, an effect of enhancing collagen synthesis, an antioxidative effect and a moisturizing effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Production Example 1: Production of a mixed extract of pomegranate, western rose flower, safflower and horsetail

1 kg of shade pale pomegranate, western rose flower, safflower and safflower was refluxed for 3 hours in a 70% (v / v) aqueous ethanol solution for 1 hour at a weight ratio of 1: 1: 1: Filtered through Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure at 50 DEG C or below and lyophilized to obtain 43 g.

Manufacturing example  2 and 3: Supercritical  Manufacture of fluid extracts and ultrasonic extracts

1 kg of shade pomegranate, western rose flower, safflower and safflower at a weight ratio of 1: 1: 1: 1 is subjected to a conventional supercritical extraction method (as a co-solvent under supercritical conditions at a temperature of about 60 ° C and a pressure of about 300 atm) Ethanol) to obtain a supercritical extract (Production Example 2). Further, 1 kg of shredded pomegranate, western rose flower, safflower and japonica at a weight ratio of 1: 1: 1: 1 was sonicated (extracted with a super sonic ultrasonic device at 25 KHz for about 2 hours at 30 캜) To obtain an ultrasonic extract (Manufacturing Example 3). In the case of Production Example 3, ethanol was used as a co-solvent.

Comparative Manufacturing Example  1: Manufacture of pomegranate extract

One kilogram of shredded pomegranate was refluxed for 3 hours with 70% (v / v) ethanol aqueous solution for 5 hours, cooled, and filtered through Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower.

Comparative Manufacturing Example  2: Production of western rose flower extract

One kilogram of shrubby western rose blossom was refluxed for 3 hours with 70% (v / v) aqueous ethanol solution for 5 hours, cooled, and filtered through Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower.

Comparative Manufacturing Example  3: Preparation of safflower extract

1 kg of shredded shade safflower was refluxed for 3 hours with 70% (v / v) aqueous ethanol solution for 5 hours, cooled down, and filtered through Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower.

Comparative Manufacturing Example  4: Manufacture of extracts

One kilogram of shredded shrimp was subjected to reflux extraction three times for 5 hours in a 70% (v / v) aqueous ethanol solution, followed by cooling and filtering with a Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 DEG C or lower.

Experimental Example  1. Mixture of pomegranate, western rose flower, MMP -1 production inhibitory effect

Experimental Example 1 measured the effect of inhibiting the production of collagenase, MMP-1, which is a collagen decomposition enzyme of Comparative Preparation Examples 1 to 4, and a mixed extract of pomegranate, rose blossom, safflower and japonica obtained in Production Examples 1, 2 and 3.

Human normal skin cells were inoculated into a 48-well microplate (Nunc, Denmark) at a concentration of 1 × 10 ⁶ cells per well and cultured in DMEM medium (Sigma, USA) and 37 ° C And then cultured for 24 hours. Then, the serum-free DMEM medium and the control group in which the pomegranate, the rose blossom, the safflower flower, the mixed extract of Safflower, and the corresponding extracts of Preparation Examples 1 to 3 and the extracts of Comparative Preparations 1 to 4 were respectively added and the pomegranate, The cells were further cultured for 48 hours in serum-free DMEM medium containing no blossom, safflower and hyacinth extract.

After culturing, the supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) The inhibition rate (%) was calculated and the results are shown in Table 1.

Name of sample Treatment concentration Inhibition rate of MMP-1 production (%) Production Example 1 Production Example 2 Production Example 3 Comparative Preparation Example 1 Comparative Production Example 2 Comparative Production Example 3 Comparative Production Example 4 extract 10ppm 22.79 24.17 23.82 9.5 7.2 5.2 6.8 100ppm 45.74 54.81 53.14 18.2 16.3 17.5 14.6 1000ppm 87.12 89.19 88.51 42.3 38.2 35.6 32.5 TGF-beta (200 nM) 76.8

As shown in the results of Table 1 above, it was confirmed that the mixed extract of pomegranate, western rose flower, safflower and Japanese white pearl of the present invention inhibits the production of MMP-1 (inhibits MMP-1 activity) in a concentration-dependent manner. (200 nM), which is a substance known to have an inhibitory effect on MMP-1 production, and the concentration (100 ppm) of the mixed extract of pomegranate, western rose flower, The inhibitory rate of MMP-1 at the level of the above-mentioned mixed extracts shows that the mixed extract of pomegranate, western rose flower, safflower, and marigold has an inhibitory effect on MMP-1 production.

Experimental Example  2. Enhancement of collagen synthesis in pomegranate, western rose flower, safflower and mixed flower extract

Human normal epithelial cells, fibroblasts, were inoculated into 48-well microplates at a density of 1 x 10 ⁶ cells per well and cultured in DMEM medium for 24 hours. The mixture of pomegranate, western rose flower, safflower and mulberry produced by Preparation Examples 1 to 3, and serum-free DMEM medium supplemented with Comparative Preparation Examples 1 to 4 and a control group were mixed with pomegranate, And further cultured in serum-free DMEM medium without extracts for 48 hours. After the incubation, the supernatant of each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan) and converted into ng / Respectively. Collagen biosynthesis increase rate (%) was calculated according to the following formula (2), and the results are shown in Table 2.

Name of sample Treatment concentration Collagen biosynthesis rate (%) Production Example 1 Production Example 2 Production Example 3 Comparative Preparation Example 1 Comparative Production Example 2 Comparative Production Example 3 Comparative Production Example 4 extract 5 ppm 21.0 24.9 23.8 11.5 8.6 10.2 6.5 10ppm 43.4 44.5 42.5 20.1 15.2 18.2 13.8 20 ppm 154.4 163.5 165.1 38.5 35.4 36.5 30.4 TGF-beta (200 nM) 163.5

According to Table 2, it was confirmed that the collagen biosynthesis rate of the mixed extracts of pomegranate, western rose flower, safflower, and related plants produced by Production Examples 1, 2 and 3 of the present invention increased in a concentration-dependent manner. In particular, it was confirmed that the extract prepared in Preparation Example 2 was more effective than the extracts prepared in Preparation Examples 1 and 3.

Experimental Example  3: Mixture of pomegranate, western rose flower, DPPH  Radical scavenging analysis

In Experimental Example 3, the antioxidative effect (free radical scavenging ratio) was measured by the DPPH method in order to measure the antioxidative effect of the mixed extracts of pomegranate, rose flower, safflower and japonica obtained in Production Examples 1 to 3. The DPPH method measures the antioxidative effect by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The degree of free radical scavenging (%) at a wavelength of 560 nm is measured by comparing the degree of decrease of the absorbance by DPPH reduction by the test substance (mixed extract) with the absorbance of the blank test solution. As a reagent used, 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 ml.

As a measuring method,

(1) Add 0.15 ml of the sample solution to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stir rapidly, and start culturing at 25 ° C for 10 minutes.

② Measure the absorbance St at 560 nm thereafter.

(3) The blank of the sample solution is measured by the same procedure as that for measuring the absorbance St except that methanol is used instead of the 0.1 mM DPPH solution.

(4) The absorbance Bt is measured by operating in the same manner as in the measurement of the absorbance St except that distilled water is used instead of the sample solution.

(5) Blank of the blank test is carried out in the same manner as in the measurement of the absorbance St, except that methanol is used instead of the 0.1 mM DPPH solution and distilled water is used instead of the sample solution, and the absorbance Bo is measured.

The results of the free radical scavenging rate (%) were calculated according to Equation (3), and the results are shown in Table 3 below.

St: absorbance at 514 nm after free radical scavenging of the sample solution

Bt: Absorbance at 514 nm after free radical scavenging of blank test solution

So: the absorbance at 514 nm before the reaction in the absence of free radicals in the sample solution

Bo: absorbance at 514 nm before the reaction in the absence of the free radical of the blank test solution

Name of sample SC ₅₀ (%) Production Example 1 0.021 Production Example 2 0.015 Production Example 3 0.023

SC ₅₀ , which is the concentration of the sample required to remove 50% of the DPPH free radical, was confirmed. As a result, Production Examples 1 to 3 showed antioxidative effects.

Formulation Example  1: containing pomegranate, western rose flower, mixed extract of safflower Cosmetics  Preparation of composition

Cosmetic preparations (Formulation Examples 1 to 3) containing the pomegranate, rose blossom, safflower and safflower mixture extracts of Preparation Examples 1 to 3 as cosmetic preparations containing pomegranate, western rose flower, (Comparative Preparation Example 1), Western Rose Flower Extract (Comparative Preparation Example 2), Safflower Extract (Comparative Preparation Example 3) and Comparative extract (Comparative Preparation Example 4) for comparison of efficacy Formulation Examples 1 to 4 were prepared and a cosmetic composition (Comparative Formulation Example 5) containing glycerin and 1,3-butylene glycol as a moisturizing agent instead of the mixed extract of pomegranate, western rose flower, . ≪ / RTI >

division ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 compare
Formulation Example 1 compare
Formulation Example 2 compare
Formulation Example 3 compare
Formulation Example 4 compare
Formulation Example 5 A Production Example 1 5.0 - - - - - - - Production Example 2 - 5.0 - - - - - - Production Example 3 - - 5.0 - - - - - Comparative Preparation Example 1 - - - 5.0 - - - - Comparative Production Example 2 - - - - 5.0 - - - Comparative Production Example 3 - - - - 5.0 - - Comparative Production Example 4 - - - - - 5.0 - glycerin - - - - - - - 5.0 1,3-butylene glycol - - - - - - - 5.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 B Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 C Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Experimental Example  4: Moisturizing effect of mixed extracts of pomegranate, western rose flower, safflower and green tea

The clinical moisturizing effects of the cosmetic compositions of Formulation Examples 1, 2 and 3 were measured as follows. A, B, and C groups were divided into 5 groups (A, B, C, D, E, F, G and H) (Comparative Formulation Example 1) containing only pomegranate extract (Comparative Formulation Example 1), Group E: Cosmetic composition containing only rose flower extract (Comparative Formulation Example 2), Group F: Safflower (Comparative Formulation Example 3), Comparative Example 3 containing Comparative Example 3, Cosmetic composition containing only extracts (Comparative Formulation Example 3), Cosmetic composition containing Comparative Example 4 only in Group G (Comparative Formulation Example 4), H Group containing glycerin , And a cosmetic composition containing only a humectant of 1,3-butylene glycol (Comparative Formulation Example 5) were applied to the face for 2 weeks twice a day, respectively. The moisturizing effect was evaluated by the Corneometer CM 820 (Corage + Khazaka, Germany) after every week and after the test. The experimental results are shown in Table 5 below.

division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 21.4 35.6 45.7 Formulation Example 2 23.1 37.7 48.9 Formulation Example 3 24.1 38.5 47.7 Comparative Formulation Example 1 23.7 27.7 30.5 Comparative Formulation Example 2 23.8 29.4 35.9 Comparative Formulation Example 3 23.5 27.4 29.9 Comparative Formulation Example 4 22.5 25.6 27.5 Comparative Formulation Example 5 21.6 28.3 30.1

(Comparative Formulation Example 5) and cosmetic compositions (Comparative Formulation Examples 1 to 4) each containing a pomegranate extract, a Western rose flower extract, a safflower extract and a corresponding extract as shown in Table 5 above (Formulation Examples 1 to 3) containing the mixed extract of pomegranate, western rose flower, safflower, and Japanese peony of the present invention were excellent in moisturizing effect.

Experimental Example  5: Measurement of skin elasticity improvement effect

Experimental Example 5 was conducted in order to examine the skin elasticity improving effect of the cosmetic composition according to the formulation examples and the comparative formulations obtained in Preparation Examples 1 to 3, including the mixed extracts of pomegranate, rose flower, safflower, The subjects were 70 women who had decreased strength. Ten groups were divided into seven groups. Formulation 1 was applied to Group 1, Formulation Example 2 was applied to Group 2, Formulation Example 3 was applied to Group 3, Comparative Formulation Example 1 was applied to Group 4, Comparative Formulation Example 2 was applied to Group 5 , Comparative Formulation Example 3 in Group 6, and Comparative Formulation Example 4 in Group 7 were applied to the face in the morning and evening for 12 weeks, respectively, and skin elasticity was measured after 12 weeks. The elasticity of the facial region was measured using a cutometer SEM575 (MPA 580, Courage + Khazaka GmbH, Germany), and the R2 (biological elasticity) indicating skin elasticity was measured before and after application . The results are shown in Table 6 below, and the results are an average value of improvement for each group.

Experimental product Skin elasticity improvement (%) Formulation Example 1 23.5 Formulation Example 2 28.1 Formulation Example 3 26.8 Comparative Formulation Example 1 13.2 Comparative Formulation Example 2 12.2 Comparative Formulation Example 3 11.2 Comparative Formulation Example 4 12.3

As shown in Table 6, Formulations 1 to 3 containing the mixed extracts of pomegranate, rose blossom, safflower and safflower showed significantly improved skin elasticity as compared to Comparative Formulations 1 to 4.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (7)

Pomegranate, western rose flower, safflower, and a mixture of the same as an active ingredient. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving skin wrinkles. The cosmetic composition according to claim 1, wherein the cosmetic composition is for antioxidation. The cosmetic composition according to claim 1, wherein the cosmetic composition is for moisturizing. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving skin elasticity. 6. The cosmetic composition according to any one of claims 1 to 5, wherein the mixed extract of pomegranate, western rose flower, safflower and moss is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. 6. The method according to any one of claims 1 to 5, wherein the mixed extract of pomegranate, western rose flower, safflower and safflower is selected from the group consisting of (a) water, an anhydrous or lower alcohol having 1-4 carbon atoms, propylene glycol, (B) a supercritical extraction method or (c) an ultrasonic extraction method using an extraction solvent using an extraction solvent selected from the group consisting of glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane, . ≪ / RTI >