KR101680806B1 - Cosmetic composition for skin whitening comprising Amaranth - Google Patents
Cosmetic composition for skin whitening comprising Amaranth Download PDFInfo
- Publication number
- KR101680806B1 KR101680806B1 KR1020160014832A KR20160014832A KR101680806B1 KR 101680806 B1 KR101680806 B1 KR 101680806B1 KR 1020160014832 A KR1020160014832 A KR 1020160014832A KR 20160014832 A KR20160014832 A KR 20160014832A KR 101680806 B1 KR101680806 B1 KR 101680806B1
- Authority
- KR
- South Korea
- Prior art keywords
- amaranth
- cosmetic composition
- skin
- whitening
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 claims description 114
- 240000001592 Amaranthus caudatus Species 0.000 claims description 69
- 235000009328 Amaranthus caudatus Nutrition 0.000 claims description 69
- 235000012735 amaranth Nutrition 0.000 claims description 69
- 239000004178 amaranth Substances 0.000 claims description 69
- 230000002087 whitening effect Effects 0.000 claims description 59
- 239000002537 cosmetic Substances 0.000 claims description 58
- 238000000034 method Methods 0.000 claims description 14
- 239000004480 active ingredient Substances 0.000 claims description 11
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 241000798427 Vanderbylia fraxinea Species 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims 1
- 239000000469 ethanolic extract Substances 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 64
- 210000003491 skin Anatomy 0.000 description 57
- 238000009472 formulation Methods 0.000 description 52
- 239000000284 extract Substances 0.000 description 35
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 32
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 32
- 230000000052 comparative effect Effects 0.000 description 31
- 239000000047 product Substances 0.000 description 27
- 238000004519 manufacturing process Methods 0.000 description 24
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 22
- 230000000694 effects Effects 0.000 description 22
- 210000004027 cell Anatomy 0.000 description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- 102000003425 Tyrosinase Human genes 0.000 description 16
- 108060008724 Tyrosinase Proteins 0.000 description 16
- 229960000271 arbutin Drugs 0.000 description 16
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 16
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 12
- 238000000605 extraction Methods 0.000 description 12
- 230000002401 inhibitory effect Effects 0.000 description 12
- 239000002904 solvent Substances 0.000 description 12
- 239000001569 carbon dioxide Substances 0.000 description 11
- 229910002092 carbon dioxide Inorganic materials 0.000 description 11
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 10
- 238000011156 evaluation Methods 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 10
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 9
- -1 glycerol aliphatic ester Chemical class 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 239000006071 cream Substances 0.000 description 8
- 230000008859 change Effects 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 230000008099 melanin synthesis Effects 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 6
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 6
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 5
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 5
- 206010040880 Skin irritation Diseases 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 235000014113 dietary fatty acids Nutrition 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000194 fatty acid Substances 0.000 description 5
- 229930195729 fatty acid Natural products 0.000 description 5
- 235000011187 glycerol Nutrition 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 239000008213 purified water Substances 0.000 description 5
- 230000036556 skin irritation Effects 0.000 description 5
- 231100000475 skin irritation Toxicity 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 4
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 229960003237 betaine Drugs 0.000 description 4
- 239000006184 cosolvent Substances 0.000 description 4
- 238000002845 discoloration Methods 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 239000006210 lotion Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000000194 supercritical-fluid extraction Methods 0.000 description 4
- 239000004094 surface-active agent Substances 0.000 description 4
- 229960004441 tyrosine Drugs 0.000 description 4
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 3
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 3
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 229920002535 Polyethylene Glycol 1500 Polymers 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229920002125 Sokalan® Polymers 0.000 description 3
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 3
- 229960000458 allantoin Drugs 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- BTANRVKWQNVYAZ-UHFFFAOYSA-N butan-2-ol Chemical compound CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- SNPLKNRPJHDVJA-UHFFFAOYSA-N dl-panthenol Chemical compound OCC(C)(C)C(O)C(=O)NCCCO SNPLKNRPJHDVJA-UHFFFAOYSA-N 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 3
- ZCTXEAQXZGPWFG-UHFFFAOYSA-N imidurea Chemical compound O=C1NC(=O)N(CO)C1NC(=O)NCNC(=O)NC1C(=O)NC(=O)N1CO ZCTXEAQXZGPWFG-UHFFFAOYSA-N 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- VUYXVWGKCKTUMF-UHFFFAOYSA-N tetratriacontaethylene glycol monomethyl ether Chemical compound COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO VUYXVWGKCKTUMF-UHFFFAOYSA-N 0.000 description 3
- 238000002137 ultrasound extraction Methods 0.000 description 3
- 229940058015 1,3-butylene glycol Drugs 0.000 description 2
- 241000219318 Amaranthus Species 0.000 description 2
- 241000416162 Astragalus gummifer Species 0.000 description 2
- 240000006162 Chenopodium quinoa Species 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 208000034874 Product colour issue Diseases 0.000 description 2
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 2
- 229920001615 Tragacanth Polymers 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- SESFRYSPDFLNCH-UHFFFAOYSA-N benzyl benzoate Chemical compound C=1C=CC=CC=1C(=O)OCC1=CC=CC=C1 SESFRYSPDFLNCH-UHFFFAOYSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 235000019437 butane-1,3-diol Nutrition 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 208000016252 change in skin color Diseases 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- DQYBDCGIPTYXML-UHFFFAOYSA-N ethoxyethane;hydrate Chemical compound O.CCOCC DQYBDCGIPTYXML-UHFFFAOYSA-N 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000006072 paste Substances 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- 239000012064 sodium phosphate buffer Substances 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 239000000196 tragacanth Substances 0.000 description 2
- 235000010487 tragacanth Nutrition 0.000 description 2
- 229940116362 tragacanth Drugs 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 239000008158 vegetable oil Substances 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 description 1
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- WNWHHMBRJJOGFJ-UHFFFAOYSA-N 16-methylheptadecan-1-ol Chemical class CC(C)CCCCCCCCCCCCCCCO WNWHHMBRJJOGFJ-UHFFFAOYSA-N 0.000 description 1
- GJJVAFUKOBZPCB-UHFFFAOYSA-N 2-methyl-2-(4,8,12-trimethyltrideca-3,7,11-trienyl)-3,4-dihydrochromen-6-ol Chemical compound OC1=CC=C2OC(CCC=C(C)CCC=C(C)CCC=C(C)C)(C)CCC2=C1 GJJVAFUKOBZPCB-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000554155 Andes Species 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- AHMIDUVKSGCHAU-UHFFFAOYSA-N Dopaquinone Natural products OC(=O)C(N)CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 241000316316 Fomitella Species 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 1
- AHMIDUVKSGCHAU-LURJTMIESA-N L-dopaquinone Chemical compound [O-]C(=O)[C@@H]([NH3+])CC1=CC(=O)C(=O)C=C1 AHMIDUVKSGCHAU-LURJTMIESA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
- 244000043158 Lens esculenta Species 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- ULUAUXLGCMPNKK-UHFFFAOYSA-N Sulfobutanedioic acid Chemical compound OC(=O)CC(C(O)=O)S(O)(=O)=O ULUAUXLGCMPNKK-UHFFFAOYSA-N 0.000 description 1
- BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003388 anti-hormonal effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 229940079894 benzophenone-9 Drugs 0.000 description 1
- 229960002903 benzyl benzoate Drugs 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 150000005827 chlorofluoro hydrocarbons Chemical class 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- QDCHWIWENYCPIL-UHFFFAOYSA-L disodium;4-hydroxy-5-(2-hydroxy-4-methoxy-5-sulfonatobenzoyl)-2-methoxybenzenesulfonate Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(OC)=CC(O)=C1C(=O)C1=CC(S([O-])(=O)=O)=C(OC)C=C1O QDCHWIWENYCPIL-UHFFFAOYSA-L 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 201000005577 familial hyperlipidemia Diseases 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 230000002641 glycemic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 239000011539 homogenization buffer Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229960004337 hydroquinone Drugs 0.000 description 1
- MTNDZQHUAFNZQY-UHFFFAOYSA-N imidazoline Chemical class C1CN=CN1 MTNDZQHUAFNZQY-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- SUMDYPCJJOFFON-UHFFFAOYSA-N isethionic acid Chemical class OCCS(O)(=O)=O SUMDYPCJJOFFON-UHFFFAOYSA-N 0.000 description 1
- 230000003780 keratinization Effects 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 229940069445 licorice extract Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000008206 lipophilic material Substances 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000036564 melanin content Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 230000003061 melanogenesis Effects 0.000 description 1
- 210000002780 melanosome Anatomy 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- CQDGTJPVBWZJAZ-UHFFFAOYSA-N monoethyl carbonate Chemical compound CCOC(O)=O CQDGTJPVBWZJAZ-UHFFFAOYSA-N 0.000 description 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000001294 propane Substances 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 229960004063 propylene glycol Drugs 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000004761 scalp Anatomy 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 206010040882 skin lesion Diseases 0.000 description 1
- 231100000444 skin lesion Toxicity 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 229940031439 squalene Drugs 0.000 description 1
- TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 229930003802 tocotrienol Natural products 0.000 description 1
- 239000011731 tocotrienol Substances 0.000 description 1
- 235000019148 tocotrienols Nutrition 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000003021 water soluble solvent Substances 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A61K8/975—
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
Description
본 발명은 '아마란스(Amaranth caudatus) 또는 아마란스 추출물'을 아까시재목버섯(Fomitella fraxinea)으로 발효시킨 아마란스 발효물을 유효성분으로 함유하는 피부 미백용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition for skin whitening comprising, as an active ingredient, an amaranth fermented product obtained by fermenting Amaranth caudatus or Amaranth extract with Fomitella fraxinea .
피부의 흑화는 내적, 외적 요인에 대한 피부세포의 반응에 의하여 발생하는 것으로, 대표적인 요인은 피부가 자외선에 노출됨으로 인한 것이다. 즉, 피부가 자외선에 노출되면 티로시나제가 활성화되고, 이것은 피부조직에 존재하는 아미노산의 일종인 티로신에 작용하여 도파(dopa)를 생성시키고, 다시 도파퀴논(dopaquinone)을 생성시키는 산화과정을 통해 피부 색소세포인 멜라노사이트(Melanocyte)내의 멜라노좀(Melanosome)에서 멜라닌이라는 중합체가 합성된다. 이 멜라닌은 피부의 각질형성 세포인 케라티노사이트(Keratinocyte)로 전달되며, 각질화 과정에 의해 피부 표면에 도달하여 자외선으로부터 피부를 보호하게 된다. Blackening of the skin is caused by the reaction of the skin cells to internal and external factors. Typical factors are the exposure of the skin to ultraviolet rays. That is, when the skin is exposed to ultraviolet rays, tyrosinase is activated, which acts on tyrosine, a type of amino acid present in the skin tissue, to generate dopa, and then, through the oxidation process of generating dopaquinone, A polymer called melanin is synthesized from Melanosome in the cell, Melanocyte. This melanin is transferred to the keratinocyte, the keratinocyte of the skin, and reaches the skin surface by the keratinization process to protect the skin from ultraviolet rays.
멜라닌은 우리 인체에 없어서는 안 되는 자외선 방어제이며, 단백질, 지질, 핵산 등과 같은 생체성분을 변형시키는 다양한 라디칼을 제거하는데 효과적인 프리 라디칼 소거제의 역할을 담당한다. 하지만, 멜라닌이 국소적으로 과도하게 합성되거나 피부병변 및 노화에 따른 피부의 생리기능이 떨어지게 되면, 멜라닌이 피부 표면에 침착되어 기미, 주근깨 및 다양한 색소침착을 유발하게 된다.Melanin is an essential UV inhibitor in the human body and plays a role of a free radical scavenger effective in removing various radicals that modify biological components such as proteins, lipids, and nucleic acids. However, when melanin is over-synthesized locally, or when the skin's physiological functions due to skin lesions and aging deteriorate, melanin deposits on the skin surface causing spots, freckles and various pigmentation.
피부 흑화의 원인과 기작이 밝혀지면서, 피부 흑화 과정에 관여하는 티로시나제(Tyrosinase)의 활성 저해효과를 갖는 물질들을 화장료에 배합하거나, 멜라닌 생성과정 중의 일부 반응을 저해함으로써 멜라닌의 생성을 감소시키는 방법이 피부 흑화를 방지하기 위해 일반적으로 사용되고 있다. 이러한 목적으로 사용되고 있는 대표적인 물질로는 알부틴, 아스코르빈산, 히드로퀴논, 상백피추출물 및 감초추출물 등의 식물 추출물이 있다. As a cause and mechanism of skin blackening are revealed, a method of reducing the production of melanin by mixing substances having an inhibitory effect on tyrosinase activity involved in the skin blackening process in cosmetics or inhibiting some reactions during the melanin production process It is commonly used to prevent skin blackening. Representative materials that have been used for this purpose include plant extracts such as arbutin, ascorbic acid, hydroquinone, bark extract, and licorice extract.
그러나, 알부틴의 경우, 화장품에 배합시 변색 및 경시변화에 따른 역가 저하 등 안정성에 문제점이 있으며, 피부자극이 커 사용상 한계가 있다. 아스코르빈산은 티로시나제 활성 저해효과가 낮고, 분자 자체의 안정성이 낮아 멜라닌 생성 억제제로 적합하지 않은 문제가 있다. 히드로퀴논은 피부에 대한 자극이 높아 안전성 문제로 현재 화장품 배합시에 그 사용성이 제한되는 문제가 있다. 또한, 대부분의 식물 추출물들은 고농도에서만 비교적 높은 티로시나제 활성 저해 효과를 나타내며, 저농도에서는 저해 효과가 거의 나타나지 않는 등의 문제점을 갖고 있다. 특히, 기존 멜라닌 생성 억제제들은, 실제 사람 피부에 대한 임상적인 실험시 미백효과가 미약하다는 가장 큰 문제점이 있다. However, in the case of arbutin, there is a problem in stability such as discoloration and decrease in potency due to aging change when it is added to cosmetics, and there is a limit in use due to skin irritation. Ascorbic acid has a problem that the effect of inhibiting the activity of tyrosinase is low and the stability of the molecule itself is low, which is not suitable as an inhibitor of melanogenesis. Hydroquinone has a problem that its usability is limited when it is mixed with cosmetics at present because of high safety of skin irritation. In addition, most plant extracts exhibit relatively high inhibitory effect on tyrosinase activity only at a high concentration, and the inhibitory effect is hardly exhibited at a low concentration. In particular, the existing melanin production inhibitors have the biggest problem that the whitening effect is weak in a clinical experiment on actual human skin.
따라서, 안정성의 문제가 없고, 우수한 미백효과를 보이는 천연발효물을 함유하는 피부 미백용 화장료 조성물에 대한 연구가 꾸준히 이루어지고 있다. Accordingly, there is a continuing research on cosmetic compositions for skin whitening, which contain natural fermented products exhibiting excellent whitening effect without any problem of stability.
본 발명은 멜라닌 생성을 억제하여 미백효능이 우수한 천연발효물을 유효성분으로 함유하는 피부 미백용 화장료 조성물을 제공하는 것을 목적으로 한다.An object of the present invention is to provide a cosmetic composition for skin whitening which contains, as an active ingredient, a natural fermented product having an excellent whitening effect by inhibiting melanin production.
본 발명은 아마란스(Amaranth caudtus) 발효물을 유효성분으로 함유하는 것을 특징으로 하되, 상기 아마란스 발효물은 아마란스 또는 아마란스 추출물을 아까시재목버섯(Fomitella fraxinea)으로 발효시킨 것을 특징으로 하는 피부 미백용 화장료 조성물을 제공한다. The present invention is characterized in that a fermented product of Amaranth caudtus is contained as an active ingredient, wherein the amaranth fermented product is obtained by fermenting Amaranth or Amaranth extract with Fomitella fraxinea .
본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 발효는 바람직하게 배양 온도 20~30℃, pH 5~7 및 호기적 조건에서 8~12일간 처리되는 것이 좋다. In the cosmetic composition for skin whitening of the present invention, the fermentation is preferably performed at a culture temperature of 20 to 30 ° C, a pH of 5 to 7, and an aerobic condition for 8 to 12 days.
본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 아마란스는 일예로, 잎, 꽃잎 및 줄기 중 선택되는 어느 하나를 파쇄한 것일 수 있다. 바람직하게는 파쇄 후, 100~500 메쉬(mesh)로 여과된 것이 좋다. In the cosmetic composition for skin whitening of the present invention, the amaranth may be one obtained by disrupting any one selected from leaves, petals and stem. Preferably, after crushing, it is preferably filtered at 100 to 500 mesh.
본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 아마란스 추출물은 바람직하게, 잎, 꽃잎, 줄기 등으로부터 추출하여 얻은 것이 좋고, 더욱 바람직하게는 잎, 꽃잎을 추출하여 얻은 것이 좋다. 가장 바람직하게는 꽃잎으로부터 추출하여 얻은 것이 좋다. 이를 만족하는 아마란스 추출물을 아까시재목버섯으로 발효한 아마란스 발효물의 경우, 티로시나제 활성을 우수하게 저해하고, 멜라닌 생성을 억제하여 피부 미백에 우수한 효과를 발휘할 수 있다.In the cosmetic composition for skin whitening of the present invention, the above-described amaranth extract is preferably obtained by extracting from leaves, petals, stems and the like, more preferably obtained by extracting leaves and petals. Most preferably, it is obtained from a petal. The amaranth fermented product obtained by fermenting Amaranth extract with Amaranthus japonicus mushroom exhibits superior effects on tyrosinase activity and suppression of melanin production, thereby exerting an excellent effect on skin whitening.
본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 아마란스 추출물은 바람직하게 100~500 메쉬(mesh)로 여과된 것이 좋다. In the cosmetic composition for whitening skin of the present invention, the amaranth extract preferably is filtered at 100 to 500 mesh.
본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 아마란스 추출물은 당업계에서 공지된 통상의 방법에 따라, 즉 통상적인 온도와 압력의 조건 하에서 통상적인 용매를 사용하여 추출될 수 있다. 다만, 바람직하게는 용매 추출법, 초임계 추출법 및 초음파 추출법 중 선택되는 어느 하나의 추출법을 통해 추출된 것이 좋다. In the cosmetic composition for skin whitening of the present invention, the amaranth extract can be extracted according to a conventional method known in the art, that is, using a conventional solvent under the conditions of ordinary temperature and pressure. However, it is preferably extracted through any extraction method selected from a solvent extraction method, a supercritical extraction method and an ultrasonic extraction method.
본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 용매 추출법은 바람직하게, 물, 탄소수 1~4의 무수 또는 함수 저급 알코올, 프로필렌글리콜, 부틸렌글리콜, 글리세린, 아세톤, 에틸 아세테이트, 클로로포름, 부틸 아세테이트, 디에틸에테르, 디클로로메탄 및 헥산으로 부터 선택되는 하나 이상을 추출용매로 사용하는 것이 좋으며, 더욱 바람직하게는 70%(v/v) 에탄올 또는 물을 사용하는 것이 좋고, 가장 바람직하게는 70%(v/v) 에탄올을 추출용매로 사용하는 것이 좋다. In the cosmetic composition for skin whitening of the present invention, the solvent extraction method preferably includes water, anhydrous or lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, More preferably 70% (v / v) ethanol or water is used as the extraction solvent, and most preferably 70% (v / v) ethanol or water is used as the extraction solvent. v / v) ethanol as the extraction solvent.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 초임계 추출법은 초임계 유체 추출법(supercritical fluid extraction)을 의미하는 것으로, 일반적으로 초임계 유체는 기체가 고온 고압 조건에서 임계점에 도달하였을 때, 액체 및 기체의 성질을 지니고 있으며, 화학적으로 비극성 용매와 유사한 극성을 지니고 있다. 이러한 특성으로 인해 초임계 유체는 지용성 물질의 추출에 사용되고 있다. 초임계 유체로 사용되는 이산화탄소는 초임계 유체기기의 작동으로 압력 및 온도가 임계점까지 이르는 과정을 거치면서 액체 및 기체 성질을 동시에 지닌 초임계 유체가 되고, 그 결과 지용성 용질에 대한 용해도가 증가한다. 초임계 이산화탄소가 일정량의 시료를 함유한 추출 용기를 통과하게 되면 시료에 함유된 지용성 물질은 초임계 이산화탄소에 추출되어 나온다. In the cosmetic composition for skin whitening according to the present invention, the supercritical extraction refers to supercritical fluid extraction. Generally, supercritical fluids, when a gas reaches a critical point under high-temperature and high-pressure conditions, It has liquid and gas properties, and is chemically similar in polarity to non-polar solvents. Because of these properties, supercritical fluids are being used to extract lipophilic materials. The carbon dioxide used as the supercritical fluid becomes a supercritical fluid having the liquid and gas properties at the same time as the pressure and the temperature reach the critical temperature by the operation of the supercritical fluid device, and as a result, the solubility to the oil soluble solute increases. When supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the lipophilic substance contained in the sample is extracted into supercritical carbon dioxide.
또한, 상기 초임계 추출법은 일 예로 이산화탄소 또는 이산화탄소에 추가적으로 공용매를 혼합한 혼합유체를 초임계 유체로 사용함으로써 유효 성분을 추출할 수 있다. 상기 공용매는 일 예로 클로로포름, 에탄올, 메탄올, 물, 에틸아세테이트, 헥산 및 디에틸에테르로 이루어진 군에서 선택되는 하나 또는 그 이상의 혼합물을 사용할 수 있다. 초임계 이산화탄소를 통해 지용성 물질을 추출한 후, 추출 용기에 남아있는 시료에 다시 소량의 공용매가 함유된 초임계 이산화탄소를 흘려 통과시키면 순수한 초임계 이산화탄소만으로는 추출되지 않았던 성분들이 추출되어 나오게 할 수 있다. 또한, 초임계 이산화탄소를 이용하여 추출된 시료는 이산화탄소를 함유하고 있는데 이산화탄소는 실온에서 공기 중으로 휘발되므로 쉽게 제거할 수 있고, 공용매는 감압증발기로 제거할 수 있다.In addition, the supercritical extraction method can extract an active ingredient by using, for example, a mixed fluid in which a cosolvent is further mixed with carbon dioxide or carbon dioxide as a supercritical fluid. The co-solvent may be, for example, one or more mixtures selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether. After extracting the lipid-soluble material through the supercritical carbon dioxide, supercritical carbon dioxide containing a small amount of the co-solvent is passed through the remaining sample in the extraction vessel to extract the components that were not extracted with pure supercritical carbon dioxide alone. In addition, the sample extracted using supercritical carbon dioxide contains carbon dioxide. Since the carbon dioxide is volatilized into air at room temperature, it can be easily removed, and the co-solvent can be removed by the decompression evaporator.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 초음파 추출법은 초음파 진동에 의해 발생되는 에너지를 이용하는 추출방법으로, 수용성 용매 속에서 초음파가 시료에 포함된 불용성인 물질을 파괴시킬 수 있다. 초음파를 가하게 되면, 발생하는 높은 국부온도로 인하여 주위에 위치하는 반응물 입자들의 운동에너지가 커져 반응에 필요한 충분한 에너지를 얻게 된다. 또한, 초음파 에너지의 충격효과로 높은 압력을 유도하여 시료에 함유된 물질과 용매의 혼합 효과를 높여주어 추출효율을 증가시킬 수도 있다. 상기 초음파 추출법에 사용할 수 있는 추출용매는 클로로포름, 에탄올, 메탄올, 물, 에틸아세테이트, 헥산 및 디에틸에테르로 이루어진 군에서 선택되는 하나 또는 그 이상의 혼합물을 사용할 수 있다. 이때, 추출된 시료는 용매를 제거하기 위해, 일 예로 진공 여과하여 여과액을 회수한 후, 감압증발기를 사용할 수 있고, 동결 건조하는 방법을 사용할 수도 있다. On the other hand, in the cosmetic composition for whitening skin of the present invention, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration. In the water-soluble solvent, ultrasonic waves can destroy insoluble matter contained in the sample. When the ultrasonic wave is applied, the kinetic energy of the reactant particles located around due to the high local temperature is increased, so that sufficient energy required for the reaction is obtained. Also, the extraction efficiency can be increased by inducing a high pressure by the impact effect of the ultrasonic energy, thereby enhancing the mixing effect of the substance contained in the sample and the solvent. The extraction solvent usable in the ultrasonic extraction may be one or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane and diethyl ether. At this time, the extracted sample may be vacuum-filtered to remove the solvent, for example, recover the filtrate, and then use a vacuum evaporator or a freeze-drying method.
한편, 본 발명의 아마란스(Amaranth caudatus)는 남아메리카 안데스산맥의 고산지대에서 약 5,000년 전부터 재배되었던 일년생 비름과 식물로, '신이 내린 곡물'이라고도 불리는 슈퍼푸드 중 하나이다. 아마란스는 전체 성분 중 평균 15~17%가 식물성 단백질로 구성되어 있을 만큼 퀴노아와 비등한 단백질 함량을 나타내며, 동물성 단백질 식품인 우유를 대체할 수 있는 고단백 식품으로 손꼽힌다. 인체를 구성하는 데 필요한 필수아미노산이 모두 함유되어 있으며, 그 중 리신 성분은 간 기능 개선 효과와 함께 칼슘 흡수를 돕는 효과도 있다. Meanwhile, Amaranth caudatus of the present invention is an annual peanut plant which was cultivated about 5,000 years ago in the high mountains of the Andes of South America, and is one of the superfoods which is also called 'god grains.' Amaranth is a high-protein food that can replace milk, an animal protein food, with an average protein content comparable to quinoa, with an average of 15 to 17 percent of the total ingredients being composed of vegetable proteins. It contains all the essential amino acids needed to make the human body. Among them, lysine component has an effect of improving calcium absorption as well as improving liver function.
뿐만 아니라, 칼슘ㆍ칼륨ㆍ인ㆍ철분 등의 무기질을 비롯해 섬유질, 인지질 등 다양한 영양성분이 풍부하여 다른 곡물에 부족한 영양소를 보충할 수 있는 건강식품이다. 쌀, 보리, 밀 등 다른 곡류에 비해 탄수화물이나 나트륨 함량이 낮고, 글루텐이 전혀 없어서 체중 조절용 식품으로도 탁월하다. 특히, 식물성 스쿠알렌ㆍ폴리페놀ㆍ토코트리에놀 등 항산화 및 항암 성분이 다량 함유되어, 혈당을 조절하기 때문에 당뇨, 고혈압, 고지혈증에 뛰어난 효능을 보인다. 체내 활성산소를 제거하는 항산화 기능은 면역력 증강, 노화 방지를 비롯해 호르몬 조절 효과로 피부 미용에도 도움을 준다. 더불어 불포화지방산과 식물성스테롤(phytosterol)의 높은 함유량 또한 혈중 콜레스테롤 수치와 혈당지수(GI : glycemic index)를 낮춰 당뇨, 고혈압, 동맥경화를 예방한다. 이처럼 아마란스는 각종 성인병 예방과 항암 작용에 효과적인 완전식품으로 각광받으며, 어린이 이유식, 다이어트 식품, 채식주의자들의 영양식품 등으로 활용되고 있다.In addition, it is rich in various nutrients such as calcium, potassium, phosphorus and iron, fiber, and phospholipid, and is a health food that can supplement nutrients in other grains. Compared to other grains such as rice, barley, and wheat, it has low carbohydrate and sodium content, and has no gluten. Especially, it contains antioxidant and anti-cancer ingredients such as vegetable squalene, polyphenol, and tocotrienol, and regulates blood sugar. Therefore, it shows excellent effects on diabetes, hypertension and hyperlipemia. The antioxidant function that removes active oxygen in the body helps the skin beauty by the immunity enhancement, anti-aging and hormone control effect. In addition, the high content of unsaturated fatty acids and phytosterol also lowers blood cholesterol and glycemic index (GI) to prevent diabetes, hypertension and arteriosclerosis. As such, amaranth is well-known as a complete food for effective prevention of various diseases and anticancer effects, and is being used for children's baby food, diet food, and nutritional food for vegetarians.
한편, 본 발명의 아까시재목버섯(Fomitella fraxinea)은 장수버섯이라고도 불리며, 일년생으로 갓은 지름이 5~20 cm, 두께 1~2 cm 정도이다. 처음에는 반구형이며, 연한 황색 또는 난황색의 혹처럼 덩어리진 모양으로 발생하였다가 생장하면서 반원형으로 편평해진다. 갓 표면은 적갈색이나 차차 흑갈색이 되며, 각피화된다. 갓 가장자리는 성장하는 동안 연한 황색이고, 환문이 있다. 조직은 코르크질이고 연한 황갈색이다. 자실층은 황색에서 회백색으로 되며, 상처를 주면 검은 갈색의 얼룩이 생긴다. 관공은 한 개의 층으로 형성되며, 길이는 0.3~1cm 정도이고, 관공구는 원형으로 조밀하다. 포자문은 백색이며, 포자모양은 난형이고 두꺼운 벽을 가지고 있다.On the other hand, the fomitella mushroom of the present invention fraxinea ) is also called longevity mushroom, which is a year-old god, 5-20 cm in diameter and 1-2 cm thick. It is hemispheric at first and occurs in a lumpy shape like a pale yellow or yellowish yellow lump and grows in a semicircular shape with growth. The surface is reddish brown, gradually dark brown, and becomes pebbly. Freshly marginal is pale yellow while growing, and has a discourse. The tissue is corky and light tan. The fascial layer turns from yellow to off-white, and a black-brown stain is formed when a wound is applied. The hole is formed as one layer, about 0.3 ~ 1cm in length, and the tube tool is circular and dense. Formulation is white, spore is ovate and has thick wall.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 상기 아마란스 발효물은, 바람직하게 화장료 조성물 전체 중량에 대하여 0.001~30.0중량% 함유되는 것이 좋다. 더욱 바람직하게는 화장료 조성물 전체 중량에 대하여 0.01~10중량% 함유되는 것이 좋다. 상기 추출물의 함량이 0.001중량% 미만인 경우에는 피부 미백 개선 효과가 나타나지 않으며, 30.0중량%를 초과하는 경우에는 함유량 증가에 대한 피부 미백 개선 효과 증대 정도가 미미하고, 제형상의 안전 및 안정성에 문제가 있으며, 경제적이지 않다. On the other hand, in the cosmetic composition for whitening skin of the present invention, the amaranth fermentation product is preferably contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. More preferably 0.01 to 10% by weight based on the total weight of the cosmetic composition. When the content of the extract is less than 0.001% by weight, the effect of improving skin whitening is not exhibited. When the content of the extract is more than 30.0% by weight, the degree of improvement of skin whitening effect is small, It is not economical.
한편, 본 발명의 피부 미백용 화장료 조성물은 화장료 조성물에 통상적으로 이용되는 성분들을 포함할 수 있으며, 일 예로 항산화제, 안정화제, 용해화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 그리고 담체를 포함한다.Meanwhile, the skin whitening cosmetic composition of the present invention may contain components commonly used in cosmetic compositions, and examples thereof include conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers .
한편, 본 발명의 피부 미백용 화장료 조성물은 당업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 일 예로 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 팩, 마사지크림 및 스프레이 등으로 제형화 될 수 있으나, 이에 한정되는 것은 아니다. 보다 상세하게는, 유연 화장수, 영양 화장수, 영양 크림, 마사지 크림, 에센스, 아이 크림, 클렌징크림, 클렌징 폼, 클렌징 워터, 팩, 스프레이 또는 파우더의 제형으로 제조될 수 있다.Meanwhile, the skin whitening cosmetic composition of the present invention can be prepared in any formulations conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants - containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라가칸트, 셀룰로오스 유도체, 폴리에틸렌글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.On the other hand, in the cosmetic composition for skin whitening of the present invention, when the formulation is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, , Silica, talc or zinc oxide may be used.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되고, 일 예로 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸렌글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌글리콜 또는 소르비탄의 지방산 에스테르가 이용될 수 있다. On the other hand, when the formulation is a solution or emulsion in the skin whitening cosmetic composition of the present invention, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, Benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소 결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라가칸트 등이 이용될 수 있다.On the other hand, in the case of the skin whitening cosmetic composition of the present invention, when the formulation is a suspension, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene A suspending agent such as sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. may be used.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.On the other hand, in the cosmetic composition for skin whitening of the present invention, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component when the formulation is a powder or a spray, In addition, it may additionally include propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 제형이 계면활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.On the other hand, in the cosmetic composition for skin whitening of the present invention, when the formulation is a surfactant-containing cleansing agent, alcohols such as aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.
한편, 본 발명의 피부 미백용 화장료 조성물에 있어서, 계면활성제 함유된 클렌징 제형 또는 계면활성제 비함유 클렌징 제형 또는 비누일 경우, 피부에 도포한 후, 닦아내거나 떼거나 물로 씻어낼 수도 있다. 일 예로, 상기 비누는 액상비누, 가루비누, 고형비누 및 오일비누이며, 상기 계면활성제 함유 클렌징 제형은 클렌징 폼, 클렌징 워터, 클렌징 수건 및 클렌징 팩이며, 상기 계면활성제 비 함유 클렌징 제형은 클렌징크림, 클렌징 로션, 클렌징 워터 및 클렌징 겔이며, 이에 한정되는 것은 아니다.On the other hand, in the cosmetic composition for whitening skin of the present invention, if it is a cleansing formulation containing a surfactant or a cleansing formulation without a surfactant or soap, it may be applied to the skin and then wiped off or washed off with water. The surfactant-free cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.
한편, 본 발명의 피부 미백용 화장료 조성물은 본 발명 이외의 다른 화장료 조성물과 중복하여 사용할 수 있다. 또한, 본 발명에 따른 화장료 조성물은 통상적인 사용방법에 따라 사용될 수 있으며, 사용자의 피부 상태 또는 취향에 따라 그 사용횟수를 달리할 수 있다.Meanwhile, the cosmetic composition for skin whitening of the present invention may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and may be used in a different number of times depending on the skin condition or taste of the user.
본 발명의 피부 미백용 화장료 조성물에 함유된 아마란스(Amaranth caudatus) 발효물은 티로시나제 활성을 저해하고, 멜라닌 생성을 억제하여 피부 미백에 우수한 효과를 발휘한다.The Amaranth caudatus fermented product contained in the skin whitening cosmetic composition of the present invention inhibits tyrosinase activity and inhibits melanin production, thereby exerting an excellent effect on skin whitening.
이하, 본 발명의 내용에 대해 하기 실시예에서 더욱 상세히 설명하지만, 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니고, 이와 등가의 기술적 사상의 변형까지를 포함한다.Hereinafter, the contents of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited to the following examples, but includes modifications of equivalent technical ideas.
[[ 제조예Manufacturing example 1: One: 아마란스amaranth (( Amaranth Amaranth caudatuscaudatus ) 추출물의 제조]) ≪ / RTI >
본 제조예에서는 70% 에탄올을 사용하여 아마란스 추출물을 제조하고자 하였다. In this example, the extract of Amaranth was prepared by using 70% ethanol.
세절하여 음건한 아마란스 잎, 꽃잎, 줄기 100 g을 분쇄하여 분쇄물을 제조하였다. 상기 분쇄물에 70% 에탄올 5 L를 첨가한 후, 냉각콘덴서가 부착된 추출기에서, 80℃ 온도로 3시간 동안 가열하여 추출하였다. 상기 추출물을 300메쉬(mesh) 여과포로 여과한 후, 와트만 2번 여과지로 다시 한번 여과하였다.100 g of finely chopped Amaranth leaves, petals and stems were pulverized to prepare pulverized products. To the pulverized product, 5 L of 70% ethanol was added, and the mixture was heated at 80 DEG C for 3 hours in an extractor equipped with a cooling condenser to extract it. The extract was filtered through a 300-mesh filter cloth, and then filtered once again with Watson-2 filter paper.
그 후, 칼슘을 넣고, 냉각콘덴서가 달린 증류장치로 60℃ 온도에서 감압 농축하여 13 g의 추출물을 수득하였다.Thereafter, calcium was added, and the mixture was concentrated under reduced pressure at a temperature of 60 DEG C by a distillation apparatus equipped with a cooling condenser to obtain 13 g of an extract.
[[ 제조예Manufacturing example 2 내지 19: 2 to 19: 아마란스amaranth (( Amaranth Amaranth caudatuscaudatus ) 추출물의 제조]) ≪ / RTI >
하기 표 1의 추출용매를 사용한 것을 제외하고는, 제조예 1에서와 동일한 방법으로 추출하여 아마란스 추출물을 얻었다. Amaranth extract was obtained by extracting in the same manner as in Preparation Example 1, except that the extraction solvent of Table 1 was used.
각 추출용매에 따른 아마란스 추출물의 건조중량은 하기 표 1에 나타내었다. The dry weight of the Amaranth extract according to each extraction solvent is shown in Table 1 below.
[[ 실시예Example 1: One: 아마란스amaranth (( Amaranth Amaranth caudatuscaudatus ) ) 발효물의Fermented 제조] Produce]
아마란스 추출물(제조예 1)을 약 10%(w/v)가 되게 정제수에 희석한 후, 아까시재목버섯 배양용 배지 1 L에 30 g을 첨가하여 30 g/L가 되도록하였다. 그 후, 아까시재목버섯 균주(Fomitella fraxinea) 5일 배양액을 2.5%(v/v)의 양으로 첨가하였다.The amaranth extract (Preparation Example 1) was diluted with purified water so as to be about 10% (w / v), and then 30 g was added to 1 L of the medium for cultivation of Robusta japonica mushroom to obtain 30 g / L. After that, the strain Fomitella fraxinea ) culture medium was added in an amount of 2.5% (v / v).
이후, 배양 온도 26℃, pH 6 및 호기적 조건에서 10일간 배양하였다. 배양 완료 후, 멸균하고, 300메쉬(mesh)로 여과하여 균체를 제거함으로써, 아마란스 발효물을 수득하였다. Then, the cells were cultured at a culture temperature of 26 ° C, pH 6, and aerobic condition for 10 days. After completion of the cultivation, the cells were sterilized, and the cells were removed by filtration through a 300-mesh mesh to obtain an Amaranth fermentation product.
[[ 실험예Experimental Example 1: One: 티로시나제Tyrosinase 활성 저해효과 분석] Active inhibition effect]
본 실험예에서는 상기 실시예 1의 아마란스 발효물의 티로시나제 활성 저해효과를 확인하고자 하였다. 이때, 제조예 1의 아마란스 추출물과, 미백제로 잘 알려진 상지추출물 및 알부틴을 비교샘플로 사용하였다.In this Experimental Example, the effect of inhibiting the tyrosinase activity of the amaranth fermentation product of Example 1 was examined. At this time, the amaranth extract of Preparation Example 1 and the topical extract and arbutin, well known as whitening agents, were used as comparative samples.
티로시나제는 생체 내에서 티로신(tyrosine)이라는 물질의 산화과정을 촉진하여 멜라닌이 생성되도록 하는 효소이다. 티로시나제는 버섯에서 분리 및 정제된 것으로, 시그마(Sigma)사에서 구입하여 사용하였다. Tyrosinase is an enzyme that stimulates the oxidation of tyrosine in vivo to produce melanin. Tyrosinase was isolated and purified from mushroom and purchased from Sigma.
기질인 L-티로신은 0.05 M 인산나트륨 완충용액(pH 6.8)에 용해하여 0.1 ㎎/㎖ 용액으로 만들고, 상기 제조예 1에서 수득한 아마란스 추출물, 실시예 1에서 수득한 아마란스 발효물을 각각 0.05 M 인산나트륨 완충용액(pH 6.8)에 10% 농도가 되도록 용해한 후, 사용하였다. The substrate, L-tyrosine, was dissolved in a 0.05 M sodium phosphate buffer solution (pH 6.8) to prepare a 0.1 mg / ml solution. The amaranth extract obtained in Preparation Example 1 and the amaranth fermentation obtained in Example 1 were each dissolved in 0.05 M Was dissolved in a sodium phosphate buffer solution (pH 6.8) so as to have a concentration of 10%, and then used.
L-티로신 용액 0.5 ㎖를 시험관에 넣고, 여기에 아마란스 추출물 용액, 아마란스 발효물 용액 및 알부틴 0.5 ㎖를 각각 가한 후, 37℃ 항온기에서 10분간 방치하였다. 0.5 ml of L-tyrosine solution was placed in a test tube, and the amaranth extract solution, the amaranth fermentation solution and arbutin 0.5 ml were added thereto, and the mixture was left at 37 ° C for 10 minutes.
그 후, 200 unit/㎖ 티로시나제 0.5 ㎖를 첨가하고, 37℃에서 10분간 반응시켰다. 이 반응액이 든 시험관을 얼음 위에 놓아 급냉시켜 반응을 중지시킨 후, 분광광도계를 파장 475 nm에서의 흡광도를 측정하였다. Thereafter, 0.5 ml of 200 units / ml tyrosinase was added, and the mixture was reacted at 37 占 폚 for 10 minutes. The reaction tube was placed on ice to quench the reaction, and the absorbance at 475 nm was measured by a spectrophotometer.
대조군으로는 아마란스 발효물 대신 완충용액 0.5 ㎖를 넣은 것을 사용하였다. 이때, 티로시나제에 대한 활성 저해율(%)은 하기 수학식 1에 의하여 계산하였으며, 티로시나제 활성이 50% 저해될 때의 IC50의 값을 각각 측정하였다. 그 결과는 하기의 표 2에 나타내었다.As a control group, 0.5 ml of a buffer solution was used instead of the fermentation of amaranth. At this time, the inhibitory activity (%) against tyrosinase was calculated by the following equation (1), and the IC 50 value when the tyrosinase activity was inhibited by 50% was measured. The results are shown in Table 2 below.
(아마란스 추출물)Production Example 1
(Amaranth extract)
(아마란스 발효물)Example 1
(Amaranth fermented product)
실험결과, 티로시나제 활성을 50% 저해하는 아마란스 추출물의 농도는 101.5 ㎍/ml이었고, 상지추출물은 236.4 ㎍/ml, 알부틴은 198.5 ㎍/ml로 측정되었다. 이와 비교하여, 본 발명의 아마란스 발효물인 실시예 1은 33.4 ㎍/ml로 측정되어, 티로시나제 활성 억제효과가 월등히 뛰어난 것을 확인할 수 있었다. As a result of the experiment, the concentration of amaranth extract inhibiting tyrosinase activity by 50% was 101.5 / / ml, that of upper body extract was 236.4 / / ml and that of arbutin was 198.5 / / ml. In comparison, Example 1, which is an amaranth fermented product of the present invention, was measured at 33.4 占 퐂 / ml, confirming that the effect of inhibiting tyrosinase activity was remarkably excellent.
[실험예 2: B16F1 멜라닌 형성 세포를 이용한 멜라닌 생성 억제효과 분석][Experimental Example 2: Analysis of inhibitory effect on melanin production using B16F1 melanin-forming cells]
본 실험예에서는 상기 실시예 1의 아마란스 발효물의 미백 효과를 확인하기 위해 B16F1 멜라닌 형성 세포에 대한 멜라닌 생성 억제 정도를 알아보고자 하였다. 이때, 제조예 1의 아마란스 추출물과, 미백제로 잘 알려진 알부틴을 비교샘플로 사용하였다.In order to confirm the whitening effect of the fermented amaranthus of Example 1, the present experimental example was conducted to investigate the inhibition of melanin formation on B16F1 melanin-forming cells. At this time, the amaranth extract of Preparation Example 1 and arbutin, which is well known as a whitening agent, were used as a comparative sample.
본 실험예에서 사용한 B16F1 멜라닌 형성 세포는 마우스에서 유래한 세포로, ATCC(American Type Culture Collection)로부터 분양받아 사용하였다. B16F1 멜라닌 형성 세포를 6웰 플레이트에 각 웰당 2×105 농도로 분주하고, 세포를 부착시킨 후, 독성을 유발하지 않는 농도로 분주하였다.The B16F1 melanin-forming cells used in this experiment were mouse-derived cells, and were used from ATCC (American Type Culture Collection). B16F1 melanin-forming cells were dispensed in a 6-well plate at a concentration of 2 × 10 5 cells / well, and the cells were adhered to each other at a concentration that did not cause toxicity.
그 후, 상기 제조예 1에서 수득된 아마란스 추출물, 실시예 1에서 수득된 아마란스 발효물 및 알부틴을 각각 하기 표 3에 기재된 만큼씩 처리하여 72시간 동안 배양하였다. 72시간 배양 후, 세포를 트립신-EDTA로 떼어내고, 세포수를 측정한 다음 원심분리하여 세포를 회수하였다. Thereafter, the amaranth extract obtained in Preparation Example 1, the amaranth fermentation product obtained in Example 1, and arbutin were each treated as described in Table 3 and cultured for 72 hours. After 72 hours of culture, the cells were detached with trypsin-EDTA, the number of cells was measured, and the cells were recovered by centrifugation.
세포 내 멜라닌의 정량은 로탄(Lotan: Cancer Res., 40: 3345-3350, 1980)의 방법을 변형하여 실시하였다. 셀 펠릿을 PBS로 1회 세척한 후, 균질화 버퍼액(50 mM 소듐 포스페이트, pH6.8, 1% Triton X-100, 2 mM PMSF) 1 ml를 첨가하여 5분간 와류하여 세포를 파쇄하였다. 원심분리(3,000rpm, 10분)하여 얻은 세포 여액에 1N NaOH(10% DMSO)를 첨가하여 추출된 멜라닌을 용해한 후, 마이크로 플레이트 판독기로 405 nm에서 멜라닌의 흡광도를 측정하였다.Quantification of intracellular melanin was carried out by modification of the method of Lotan (Cancer Res., 40: 3345-3350, 1980). Cell pellet was washed once with PBS, and 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. After centrifugation (3,000 rpm, 10 min), 1N NaOH (10% DMSO) was added to the cell filtrate to dissolve the extracted melanin and the absorbance of melanin was measured at 405 nm with a microplate reader.
그 후, 멜라닌을 정량하여 시료의 멜라닌 생성 저해율(%)을 측정하였다. B16F1 멜라닌 형성 세포의 멜라닌 생성 저해율(%)은 하기 수학식 2에 의하여 계산하였으며, 그 결과를 표 3에 나타내었다. Thereafter, melanin was quantified to measure the inhibition rate (%) of melanin formation of the sample. Melanin formation inhibition rate (%) of melanin-forming cells of B16F1 was calculated by the following equation (2), and the results are shown in Table 3 below.
A: 시료를 첨가하지 않은 웰의 멜라닌 양A: Amount of melanin in wells to which no sample was added
B: 시료를 첨가한 웰의 멜라닌 양B: Amount of melanin in the well to which the sample was added
(아마란스 추출물)Production Example 1
(Amaranth extract)
(아마란스 발효물)Example 1
(Amaranth fermented product)
실험 결과, 아마란스 발효물의 농도가 50 ㎍/ml 이상이 되면서부터 멜라닌 생성 억제능이 알부틴보다 우수하게 나타나는 것을 확인할 수 있었다. 또한, 아마란스를 발효물이 아마란스 추출물보다 더욱 우수한 효과를 갖는 것을 확인하였다.As a result of the experiment, it was confirmed that the melanin production inhibitory ability was superior to the arbutin when the concentration of the fermented amaranth was 50 μg / ml or more. In addition, it was confirmed that the fermented product of amaranth has more excellent effect than the extract of amaranth.
[ [ 제형예Formulation Example 1: One: 아마란스amaranth 발효물을The fermented product 함유하는 미백용 For whitening 화장료Cosmetics 조성물 제조] Composition Preparation]
상기 실시예 1에서 수득한 아마란스 발효물 5 g, 글리세린 10 g, 베타인 5 g, PEG 1500 2g, 알란토인 0.1 g, DL-판테놀 0.3 g, EDTA-2Na 0.02 g, 벤조페논-9 0.04 g, 히드록시에틸 셀룰로오스 0.1 g, 소듐히아루로네이트 8 g, 카르복시비닐폴리머 0.2 g, 트리에탄올아민 0.18 g, 옥틸도데카논 0.3 g, 옥틸도데세스-16 0.4 g, 에탄올 6 g, 향 0.2 g, Germall 115 0.3 g, 정제수 61.86 g 를 혼합하여 미백용 화장료 조성물을 제조하였다. 5 g of the amaranth fermentation product obtained in Example 1, 10 g of glycerin, 5 g of betaine, 2 g of PEG 1500, 0.1 g of allantoin, 0.3 g of DL-Panthenol, 0.02 g of EDTA-2Na, 0.04 g of benzophenone- 0.1 g of hydroxyethylcellulose, 8 g of sodium hyaruronate, 0.2 g of carboxyvinyl polymer, 0.18 g of triethanolamine, 0.3 g of octyldodecanone, 0.4 g of octyldodeces-16, 6 g of ethanol, 0.2 g of fragrance, 0.3 g of Germall 115 , And purified water (61.86 g) were mixed to prepare a whitening cosmetic composition.
[ [ 비교제형예Comparative Formulation Example 1: One: 아마란스amaranth 추출물을 함유하는 미백용 For whitening containing extracts 화장료Cosmetics 조성물 제조] Composition Preparation]
상기 제조예 1에서 수득한 아마란스 추출물 5 g과, 글리세린 10 g, 베타인 5 g, PEG 1500 2g, 알란토인 0.1 g, DL-판테놀 0.3 g, EDTA-2Na 0.02 g, 벤조페논-9 0.04 g, 히드록시에틸 셀룰로오스 0.1 g, 소듐히아루로네이트 8 g, 카르복시비닐폴리머 0.2 g, 트리에탄올아민 0.18 g, 옥틸도데카논 0.3 g, 옥틸도데세스-16 0.4 g, 에탄올 6 g, 향 0.2 g, Germall 115 0.3 g, 정제수 61.86 g 를 혼합하여 미백용 화장료 조성물을 제조하였다. 5 g of the amaranth extract obtained in Preparation Example 1 and 10 g of glycerin, 5 g of betaine, 2 g of PEG 1500, 0.1 g of allantoin, 0.3 g of DL-Panthenol, 0.02 g of EDTA-2Na, 0.04 g of benzophenone- 0.1 g of hydroxyethylcellulose, 8 g of sodium hyaruronate, 0.2 g of carboxyvinyl polymer, 0.18 g of triethanolamine, 0.3 g of octyldodecanone, 0.4 g of octyldodeces-16, 6 g of ethanol, 0.2 g of fragrance, 0.3 g of Germall 115 , And purified water (61.86 g) were mixed to prepare a whitening cosmetic composition.
[[ 비교제형예Comparative Formulation Example 2: 알부틴을 함유하는 미백용 2: For whitening containing arbutin 화장료Cosmetics 조성물 제조] Composition Preparation]
알부틴 2 g, 글리세린 10 g, 베타인 5 g, PEG 1500 2g, 알란토인 0.1 g, DL-판테놀 0.3 g, EDTA-2Na 0.02 g, 벤조페논-9 0.04 g, 히드록시에틸 셀룰로오스 0.1 g, 소듐히아루로네이트 8 g, 카르복시비닐폴리머 0.2 g, 트리에탄올아민 0.18 g, 옥틸도데카논 0.3 g, 옥틸도데세스-16 0.4 g, 에탄올 6 g, 향 0.2 g, Germall 115 0.3 g, 정제수 64.86 g 를 혼합하여 에센스 제형의 미백용 화장료 조성물을 제조하였다.2 g of arbutin, 10 g of glycerin, 5 g of betaine, 2 g of PEG 1500, 0.1 g of allantoin, 0.3 g of DL-Panthenol, 0.02 g of EDTA-2Na, 0.04 g of benzophenone-9, 0.1 g of hydroxyethylcellulose, 8 g of Nate, 0.2 g of carboxyvinyl polymer, 0.18 g of triethanolamine, 0.3 g of octyldodecanone, 0.4 g of octyldodeces-16, 6 g of ethanol, 0.2 g of fragrance, 0.3 g of Germall 115 and 64.86 g of purified water were mixed, To prepare a whitening cosmetic composition.
[[ 실험예Experimental Example 3: 피부 미백용 3: for skin whitening 화장료Cosmetics 조성물의 미백 효능 분석] Analysis of whitening efficacy of the composition]
본 실험예에서는 상기 제형예 1의 '아마란스 발효물을 유효성분으로 함유하는 피부 미백용 화장료 조성물'의 미백 효능을 알아보고자 하였다. 이때, 상기 비교제형예 1 및 비교제형예 2의 화장료 조성물을 비교샘플로 사용하였다. In this Experimental Example, the whitening efficacy of the cosmetic composition for skin whitening containing 'an amaranth fermented product as an active ingredient' of Formulation Example 1 was examined. At this time, the cosmetic composition of Comparative Formulation Example 1 and Comparative Formulation Example 2 was used as a comparative sample.
임상 실험을 위하여, 색소침착증을 가지고 있는 건강한 성인 여성 40명을 무작위로 20명씩 2개 그룹(A, B 및 C)으로 나눈 후, A 그룹을 대상으로 비교제형예 1의 화장료 조성물을, B 그룹을 대상으로 제형예 1의 화장료 조성물, C 그룹을 대상으로 비교제형예 2를 각각 1일 2회씩 8주간 도포하였다. For clinical trials, 40 healthy adult women with pheochromocytosis were randomly divided into two groups (A, B, and C) by 20 individuals, and then the cosmetic composition of Comparative Formulation Example 1 was administered to Group A, Were applied to the cosmetic composition of Formulation Example 1 and Comparative Formulation Example 2 for C group for 8 weeks twice a day, respectively.
이후, 피부색의 변화를 분광광도계(spectrophotometer, Minolta CM600-d)를 이용하여 색의 밝기 변화(ΔL)를 측정하고, 복수의 숙련자에 의한 객관적 육안관찰과 피검자에 의한 주관적 육안관찰을 실시하여 미백효과를 측정하였다. 그 결과는 하기 표 4에 나타내었다. 이때, 미백 효능 정도를 다음의 미백 효능 평가 기준에 따라 분류하여 평가하였다. Subsequently, the change in skin color was measured by using a spectrophotometer (Minolta CM600-d), and the change in color brightness (? L) was measured. Objective visual observation with a plurality of experts and subjective visual observation with the subject were performed, Were measured. The results are shown in Table 4 below. At this time, the degree of whitening efficacy was classified and evaluated according to the following whitening efficacy evaluation criteria.
<미백효능 평가 기준><Whitening efficacy evaluation standard>
-3: 매우악화 -2: 악화 -1: 약간 악화 0: 변화 없음-3: very bad -2: worse -1: slightly worse 0: no change
1: 약간 개선 2: 개선 3: 매우 개선 1: Slight improvement 2: Slight improvement 3: Slight improvement
(제형예 1)Group A
(Formulation Example 1)
(비교제형예 1)Group B
(Comparative Formulation Example 1)
(비교제형예 2)C group
(Comparative Formulation Example 2)
(제형예 1)Group A
(Formulation Example 1)
(비교제형예 1)Group B
(Comparative Formulation Example 1)
(비교제형예 2)C group
(Comparative Formulation Example 2)
(제형예 1)Group A
(Formulation Example 1)
(비교제형예 1)Group B
(Comparative Formulation Example 1)
(비교제형예 2)C group
(Comparative Formulation Example 2)
실험 결과, 본 발명의 '아마란스 발효물을 함유하는 피부 미백 화장료 조성물'을 도포한 A 그룹의 평균은 ΔL 값은 3.54 (p<0.05)이었고, '아마란스 추출물을 함유하는 피부 미백 화장료 조성물'을 도포한 B 그룹의 평균 ΔL 값은 3.3 (p<0.05)이었고, 알부틴을 함유한 피부 미백 화장료 조성물을 도포한 C그룹의 평균 ΔL 값은 2.405 (p<0.05)이었다. As a result, the average value of ΔL of the group A applied with the skin whitening cosmetic composition containing the fermented product of the present invention was 3.54 (p <0.05), and the skin whitening cosmetic composition containing the extract of Amaranth was applied The mean ΔL value of the B group was 3.3 (p <0.05), and the mean ΔL value of the C group applied the skin whitening cosmetic composition containing arbutin was 2.405 (p <0.05).
한편, 숙련자에 의한 미백 평가에서 숙련자에 의한 미백 평가에서 A그룹은 1.45, B그룹은 1.4, C그룹은 0.6이었으며, 피검자에 의한 미백 평가에서는 A그룹은 1.4, B그룹은 1.1, C그룹은 0.6으로 나타났다.On the other hand, in the whitening evaluation by the skilled person, in the whitening evaluation by the expert, 1.45 for the A group, 1.4 for the B group and 0.6 for the C group, whitening evaluation by the subject was 1.4 for the A group, 1.1 for the B group and 0.6 Respectively.
이와 같은 결과를 통해, 본 발명의 아마란스 발효물을 함유하는 화장료 조성물의 피부 미백 효과가 가장 우수한 것을 확인할 수 있었다. From these results, it was confirmed that the skin whitening effect of the cosmetic composition containing the amaranth fermented product of the present invention was the most excellent.
[[ 실험예Experimental Example 4: 제형의 안정성 실험] 4: Stability test of formulation]
본 실험예는 상기 제형예 1의 '아마란스 발효물을 유효성분으로 함유하는 피부 미백용 화장료 조성물'의 제형의 안정성을 알아보고자 하였다. 이때, 상기 비교제형예 1의 아마란스 추출물을 유효성분으로 함유하는 화장료 조성물과 상기 비교제형예 2의 알부틴을 함유하는 화장료 조성물을 비교샘플로 사용하였다. The present experimental example was conducted to investigate the stability of the formulation of the cosmetic composition for skin whitening comprising the fermented product of Amaranth as an active ingredient in the above-mentioned Formulation Example 1. At this time, a cosmetic composition containing the amaranth extract of comparative formulation example 1 as an active ingredient and a cosmetic composition containing arbutin of comparative formulation example 2 were used as comparative samples.
상기 제형예 1, 비교제형예 1, 비교제형예 2의 피부 미백용 화장료 조성물을 각각 불투명 초자 용기에 담아, 45℃로 일정하게 유지되는 항온조에서 2주 동안 보관하였다. The cosmetic compositions for skin whitening of Formulation Example 1, Comparative Formulation Example 1 and Comparative Formulation Example 2 were respectively packed in an opaque glazed container and stored in a thermostat kept constant at 45 캜 for 2 weeks.
또한, 상기 제형예 1, 비교제형예 1, 비교제형예 2의 피부 미백용 화장료 조성물을 각각 불투명 초자용기에 담아, 4℃로 일정하게 유지되는 완전히 차광된 냉장고 내에서 2주 동안 보관한 후, 변색 정도를 비교측정하였다. The cosmetic compositions for skin whitening in Formulation Example 1, Comparative Formulation Example 1 and Comparative Formulation Example 2 were each placed in an opaque glazed container and stored for 2 weeks in a completely shaded refrigerator kept constant at 4 캜, The degree of discoloration was compared and measured.
실험 결과를 하기 표 5에 나타내었다. 이때, 제품 변색 정도는 다음의 제품 변색 평가 기준에 따라 분류하여 평가하였다. The experimental results are shown in Table 5 below. At this time, the discoloration degree of the product was classified and evaluated according to the following product discoloration evaluation standard.
<제품 변색 평가 기준>≪ Evaluation criteria of product discoloration >
0: 변화없음 1: 극히 조금 변함 2: 조금 변함 3: 조금 심하게 변함 0: No change 1: Extremely slightly changed 2: Slightly changed 3: Slightly changed
4: 심하게 변함 5: 극히 심하게 변함4: Severely changed 5: Extremely severe
실험 결과, 본 발명의 아마란스 발효물을 함유하는 피부 미백용 화장료 조성물은 변색이 거의 일어나지 않아, 제형의 안정성이 알부틴보다 훨씬 우수함을 알 수 있었다. As a result of the experiment, it was found that the skin whitening cosmetic composition containing the amaranth fermentation product of the present invention hardly discolored and the stability of the formulation is much better than that of arbutin.
[[ 실험예Experimental Example 5: 피부 자극 실험] 5: skin irritation test]
본 실험예는 상기 제형예 1의 아마란스 발효물을 유효성분으로 함유하는 피부 미백용 화장료 조성물의 피부자극 정도를 알아보고자 하였다. 이때, 상기 비교제형예 1의 아마란스 추출물을 유효성분으로 함유하는 화장료 조성물과 상기 비교제형예 2의 알부틴을 함유하는 화장료 조성물을 비교샘플로 사용하였다. The experimental example was to examine the degree of skin irritation of the skin whitening cosmetic composition containing the fermented product of Amaranth as the active ingredient of the above-mentioned Formulation Example 1. At this time, a cosmetic composition containing the amaranth extract of comparative formulation example 1 as an active ingredient and a cosmetic composition containing arbutin of comparative formulation example 2 were used as comparative samples.
성인 남,녀 20명을 대상으로 등부위에 5×20 cm 크기로 각 시료의 일정량 (20 ㎕)을 1시간 또는 24시간 동안 첩포한 후, 제거하였다. Twenty adults (20 ㎕) of each sample (5 × 20 cm) were applied to the back of the adult for 1 hour or 24 hours and then removed.
그 후, 육안으로 피부상태 변화를 판독하였다. 그 결과는 표 6에 나타내었다. 이때, 피부상태의 변화 정도는 다음의 피부상태 평가 기준에 따라 분류하여 평가하였다. Thereafter, changes in skin condition were visually observed. The results are shown in Table 6. At this time, the degree of change of the skin condition was classified and evaluated according to the following skin condition evaluation standard.
<피부 상태 평가 기준><Criteria for skin condition>
0: 변화없음 1: 극히 조금 변함 2: 조금 변함 3: 조금 심하게 변함 0: No change 1: Extremely slightly changed 2: Slightly changed 3: Slightly changed
4: 심하게 변함 5: 극히 심하게 변함4: Severely changed 5: Extremely severe
실험 결과, 본 발명의 아마란스 발효물을 유효성분으로 함유하는 피부 미백용 화장료 조성물이 비교제형예 1 및 비교제형예 2의 화장료 조성물에 비해 피부 자극이 적은 것으로 확인할 수 있었다.As a result, it was confirmed that the skin whitening cosmetic composition containing the fermented product of Amaranth according to the present invention as an active ingredient had less skin irritation than the cosmetic composition of Comparative Formulation Example 1 and Comparative Formulation Example 2. [
Claims (4)
상기 아마란스 발효물은,
아마란스 꽃잎, 잎, 줄기 중 선택되는 하나 이상의 에탄올 추출물을 아까시재목버섯(Fomitella fraxinea)으로 발효시킨 것을 특징으로 하는 피부 미백용 화장료 조성물.
( Amaranth caudtus ) fermented product as an active ingredient,
The amaranth fermentation product,
A cosmetic composition for skin whitening characterized in that at least one ethanol extract selected from amaranth petals, leaves and stems is fermented with Fomitella fraxinea .
상기 발효는,
배양 온도 20∼30℃, pH 5∼7 및 호기적 조건에서 8∼12일간 처리되는 것을 특징으로 하는 피부 미백용 화장료 조성물.
The method according to claim 1,
The above-
Wherein the composition is treated at a culturing temperature of 20 to 30 DEG C, a pH of 5 to 7, and an aerobic condition for 8 to 12 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020160014832A KR101680806B1 (en) | 2016-02-05 | 2016-02-05 | Cosmetic composition for skin whitening comprising Amaranth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020160014832A KR101680806B1 (en) | 2016-02-05 | 2016-02-05 | Cosmetic composition for skin whitening comprising Amaranth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR101680806B1 true KR101680806B1 (en) | 2016-11-30 |
Family
ID=57707026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020160014832A Active KR101680806B1 (en) | 2016-02-05 | 2016-02-05 | Cosmetic composition for skin whitening comprising Amaranth |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR101680806B1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102725138B1 (en) * | 2023-10-30 | 2024-11-05 | 김윤영 | Nanovitamin complex composition |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009102242A (en) * | 2007-10-22 | 2009-05-14 | Kose Corp | Skin-lightening agent, and external preparation for skin-lightening |
-
2016
- 2016-02-05 KR KR1020160014832A patent/KR101680806B1/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009102242A (en) * | 2007-10-22 | 2009-05-14 | Kose Corp | Skin-lightening agent, and external preparation for skin-lightening |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102725138B1 (en) * | 2023-10-30 | 2024-11-05 | 김윤영 | Nanovitamin complex composition |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101885199B1 (en) | Cosmetic composition containing the mixed extract of pomegranate, rosa centifolia flower, carthamus tinctorius and rosa rugosa | |
| KR101176528B1 (en) | Cosmetic composition comprising the extract of salvia plebeia as active ingredient | |
| KR101685251B1 (en) | Cosmetic composition comprising extract of Brassica Rapa, Brassica Rapa radish leaves or Brassica Rapa callus with anti-oxidant, astringent activity and skin wrinkle improvement effect and the manufacturing method thereof | |
| KR102063686B1 (en) | Skin external composition containing extract of soybean root | |
| KR101445642B1 (en) | Cosmetic composition comprising the extract of Lagerstroemia indica as active ingredient | |
| KR101176526B1 (en) | Cosmetic Composition Comprising the extract of Spiraea prunifolia var.simpliciflora as Active Ingredient | |
| KR102021580B1 (en) | A cosmetic composition comprising soybean sprout by product extract | |
| KR101500551B1 (en) | Cosmetic composition with hydroxycitric acid for skin whitening | |
| KR20160000093A (en) | A cosmetic composition for antioxidizing and whitening containing the extracts of germinated crops | |
| KR102226179B1 (en) | Cosmetic Compositions for Anti-aging Comprising Extracts of Plants | |
| KR102044935B1 (en) | Whitening or moisturizing cosmetic composition comprising the extract of Cicer Arietinum | |
| KR101680806B1 (en) | Cosmetic composition for skin whitening comprising Amaranth | |
| KR20210060801A (en) | Cosmetic Compositions for Anti-aging Comprising Complex Fermented Product of Plants | |
| KR102479095B1 (en) | Cosmetic composition for skin whitening comprising the extract of Styrax Japonicus | |
| KR102471009B1 (en) | Cosmetic composition containing Albiggia kalkora extract for skin whitening and improving wrinkles | |
| KR102257477B1 (en) | Cosmetic composition having Anti-pollution effect comprising Lotus leaf extracts as an effective component | |
| KR20140071716A (en) | Cosmetic composition comprising the extract of Akebia quinata as active ingredi ent | |
| KR20190030397A (en) | Cosmetic Composition Comprising Extracts of Pinecone of Pinus sylvestris | |
| KR101449577B1 (en) | Cosmetic composition for skin whitening comprising the extract of Garcinia cambogia as active ingredient | |
| KR101996547B1 (en) | Cosmetic composition with skin whitening effect containing diphylleia grayi extract | |
| KR20170018539A (en) | Skin brightening cosmetic composition with the extract of rhizomes of Belamcanda chinensis by subcritical water extraction | |
| KR20150034371A (en) | Cosmetic composition comprising the extract of Commelina communis as anti-wrinkle ingredient | |
| KR20140071713A (en) | Cosmetic composition comprising the extract of Metaplexis japonica as active ingredient | |
| KR102051581B1 (en) | Cosmetic Composition For Skin Improvement Comprising Freekeh Extracts And Fractions As Active Component | |
| KR20160004593A (en) | Cosmetic Composition Comprising Extract of Pittosporum tobira as Active Ingredient |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 20160205 |
|
| PA0201 | Request for examination | ||
| PA0302 | Request for accelerated examination |
Patent event date: 20160215 Patent event code: PA03022R01D Comment text: Request for Accelerated Examination Patent event date: 20160205 Patent event code: PA03021R01I Comment text: Patent Application |
|
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20160314 Patent event code: PE09021S01D |
|
| PE0902 | Notice of grounds for rejection |
Comment text: Final Notice of Reason for Refusal Patent event date: 20160708 Patent event code: PE09021S02D |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20161107 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20161123 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20161123 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration | ||
| FPAY | Annual fee payment |
Payment date: 20191120 Year of fee payment: 4 |
|
| PR1001 | Payment of annual fee |
Payment date: 20191120 Start annual number: 4 End annual number: 4 |
|
| PR1001 | Payment of annual fee |
Payment date: 20201118 Start annual number: 5 End annual number: 5 |
|
| PR1001 | Payment of annual fee |
Payment date: 20211116 Start annual number: 6 End annual number: 6 |
|
| PR1001 | Payment of annual fee |
Payment date: 20231120 Start annual number: 8 End annual number: 8 |