KR101743001B1 - Cosmetic composition containing chitosan and propolis extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising chitosan and propolis extract as an active ingredient. The chitosan and propolis extract according to the present invention promotes skin cell activity, alleviates skin irritation, promotes the synthesis of skin cell proteins such as collagen, thereby improving various skin troubles caused by aging and protecting the skin It has the efficacy to give.

Description

COSMETIC COMPOSITION CONTAINING CHITOSAN AND PROPOLIS EXTRACTS [0002]

The present invention provides a cosmetic composition comprising chitosan and propolis extract as an active ingredient. Chitosan and propolis extracts are effective for skin soothing and moisturizing effects, and are much more effective when used as a composite than the efficacy of each of the ingredients of the mixture. The present invention provides a cosmetic composition capable of improving the skin safety of cosmetics by alleviating the skin irritation that may occur when the cosmetic is applied to the skin using chitosan and propolis extract.

Recently, consumers who feel that they are sensitive skin are on the rise. In the general consumer consciousness survey, 30-40% of respondents answered that they are sensitive skin, and this trend continues to increase. Factors that increase the rate of feeling sensitive skin include the increase of ultraviolet radiation dose due to destruction of the ozone layer, drying of living space, personal allergy, stress, and increase of chemical harmful substances. On the other hand, It is presumed that this natural-friendly, low-level stimulation is also contributing to the awareness of sensitive skin. Therefore, there is a growing interest in materials that alleviate the stimulation of cosmetics.

In addition, human skin experiences various troubles as aging progresses. The skin becomes dry, the elasticity decreases, the wrinkles are increased, and the skin becomes dull. It is also susceptible to weak external stimuli and takes a long time to recover.

In general, various raw materials are used to make cosmetics, and usually 20 to 50 kinds of raw materials are used. These ingredients have been proven to be safe through skin irritation tests, but may be irritating to the skin, such as inflammation, itching and allergies. These reactions do not happen to all people, but only to specific people. Thus, cosmetic manufacturers are making efforts to minimize their irritation by applying commonly used stimulants to products.

Skin irritations that can be caused by the use of cosmetics include irritation such as itching, stinging and burning, and erythema and edema. Examples of contact dermatitis include irritative contact dermatitis, allergic contact dermatitis, sensory irritation, and phototoxic dermatitis, but these are difficult to distinguish clearly and are often mixed with each other. First of all, in order for skin irritation to occur, skin penetration by cosmetics should occur first, and stimulation is activated by activation of immune system through interaction with epidermis or dermis cells. In order to alleviate such skin irritation, various methods such as a method of removing skin irritation substances have been used. However, recently, functional cosmetics have been increasingly produced, and since the functional raw material itself acts as a stimulus source, .

The functional properties of chitosan-containing products are improved cholesterol, antimicrobial activity and immunity. In Japan, folk remedies have been passed that have eaten crabs and shrimp shells from olden days, and they are effective in fever and dressing. In China's "Primordial Gangmok", crab shell powder is recommended for boils and swelling.

In 1986, according to the results of a comprehensive study on the basis and application of chitin, chitosan and related enzymes, the Ministry of Education of Japan in 1986 found that chitosan inhibited the proliferation of cancer cells and prevented hypertension, liver function recovery, cholesterol regulation, .

Currently, chitosan is used as a functional material for skin cosmetics and hair cosmetics because it has skin moisturizing properties, UV blocking effect, and hair protecting action.

In addition, research on the efficacy and efficacy of chitin and chitosan societies has been launched, and unlike other health supplements, 100% of raw materials are supplied domestically. In addition, Korea has more chitosan than king crab and shrimp, and it has a good condition to produce high quality chitosan because it has a lot of red ginseng which low extraction cost.

Propolis is a type of bee that combines bees' hind legs with a resin (resin) extracted from various kinds of trees and herbs for their survival and reproduction, and then combines them with their saliva, As a collective term for a composite of various polymeric polymers produced by the reaction, its main component is a flavonoid. Flavonoids include, but are not limited to, anosoxenes such as flavone, flavanone, flavonol, flavanol, flavanone and isoflavone and their glycosides conjugated with sugars, hundreds of anthocyanins and their glycosides conjugated with sugars, And Ryuukosan ginseng.

Propolis is B.C. It has been used by ancient Egyptians for over 300 years as an antiseptic to be used as an antiseptic for mummified antimicrobial preservatives. The efficacy of this antiseptic is due to the fact that moths, small animals or snakes, It can be confirmed from the natural observations that the original shape is preserved without being corrupted by the propolis coated by bees for many years. The effects other than preservation of propolis include cosmetic effects such as anti-cancer effect, burn healing ability, eczema, atopic dermatitis and fungus-like fungi healing ability, anti-alopecia suppression ability, mitigating or eliminating the dysmenorrhea, constipation, , Tooth decay, dental caries, and oral salt. Based on this, Korea's Ministry of Health and Welfare has recently defined as food supplement 13-25 as food supplement. In addition, for flavonoids, which are the main component of propolis, there was an increase in immunity of 5.6 times that of Dr. Kabarukina of Russia in 1969, a demonstration of stronger sterilization power than that of penicillin in Alexanderplatz in 1973, Anti-inflammatory effect, clinical trial results of ammonia efficacy at Japan Institute of Technology in 1994, activation of other systemic cells, hormone stimulation activity, treatment of scurvy by action of vitamin P, hypotensive effect of hypertensive patients, maintenance of biohormone balance Results of studies and clinical trials in various countries around the world have been reported.

Korean Patent Laid-Open Publication No. 2013-0097360 discloses a nutritive cream composition containing chitosan or a derivative thereof, and Korean Patent Publication No. 2013-0134803 discloses a moisturizing cosmetic composition containing propolis nano beacycin .

The inventors of the present invention found that when chitosan and propolis mixed extract significantly alleviated the skin irritation caused by cosmetic raw materials and found that when used in cosmetics, The present inventors have completed the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition which is excellent in skin soothing and moisturizing effect, containing chitosan and propolis extract.

Other objects and advantages of the present invention will become more apparent from the following examples and claims.

The present invention relates to a cosmetic composition comprising chitosan and propolis extract.

Preferably, the present invention relates to a cosmetic composition for preventing skin aging comprising chitosan and propolis extract as an active ingredient.

Preferably, the present invention relates to a cosmetic composition for relieving skin irritation comprising chitosan and propolis extract as an active ingredient.

Preferably, the present invention relates to a cosmetic composition for skin moisturizing comprising chitosan and propolis extract as an active ingredient.

Preferably, the chitosan and propolis extract are contained in an amount of 0.0001 to 30.0% (w / w) based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, Lt; / RTI >

The cosmetic composition comprising the chitosan and propolis extract according to the present invention inhibits the biosynthesis of IL-1α, an inflammatory cytokine, and is excellent in cell stimulation relaxation effect. In the cosmetic composition containing the same, Can be expected to relieve skin irritation and soothe.

The present invention relates to a cosmetic composition comprising chitosan and propolis extract as an active ingredient.

The chitosan or propolis extract according to the present invention is extracted with a solvent selected from the group consisting of water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol , More preferably water or an alcohol. The ethanol may preferably be 70% ethanol. A suitable amount of the extraction solvent may be 1 to 15 times, more preferably 1 to 5 times, and most preferably 3 times the dry weight of chitosan and propolis.

The mixture of chitosan and propolis extract is preferably prepared by mixing natural products and then extracting (mixing and extracting).

As extraction conditions, they are extracted at a temperature of 4-100 DEG C for 1 hour to 5 days, preferably at a temperature of 90-100 DEG C for 1 hour to 5 hours, more preferably at a temperature of 95-100 DEG C 2 hours to 3 hours.

Specifically, the chitosan and propolis mixed extract of the present invention can be prepared by a process comprising the following steps:

(a) adding to the dried chitosan and propolis a solvent selected from the group consisting of 1-5 times water, 1-4 anhydrous or lower alcohol, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, ;

(b) extracting the solvent-added chitosan and propolis of step (a) at 95-100 ° C for 1 hour to 5 hours;

(c) filtering the extract of step (b) and drying the filtrate at 65 ° C with a rotary evaporator.

When 1,3-butylene glycol is used as the extraction solvent, it is difficult to dry it using a rotary evaporator. Therefore, after the extraction step (b), the drying loss should be adjusted to 1% (w / v) do.

The chitosan and propolis extract of the present invention can be prepared in powder form by an additional process such as vacuum distillation and freeze drying or spray drying.

In a preferred embodiment of the present invention, the components contained in the cosmetic composition may contain various components that maintain the activity of the biocomponent in addition to the mixture of chitosan and propolis extract used as the active ingredient of the present invention such as a preservative, Such as buffers, stabilizers, aluminum hydroxide, aluminum phosphate, surfactants, liposomes, thickeners, etc. and ingredients commonly used in cosmetic compositions such as antioxidants, stabilizers, solubilizers, vitamins, , And carriers, and the like, but are not limited thereto.

The cosmetic composition of the present invention can be prepared by any of the formulations conventionally manufactured in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, Oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The chitosan and propolis extract may be contained in an amount of 0.0001 to 30.0% (w / w), more preferably 0.01 to 20.0% (w / w) based on the total weight of the cosmetic composition. When the extract is contained in an amount less than the above range, the effect is insignificant, and when it exceeds the above range, economical efficiency is low.

In addition, the chitosan and propolis may be mixed in a dry weight ratio of 1: 3 to 3: 1, most preferably in the same dry weight ratio.

Another object of the present invention is achieved by a cosmetic method characterized by applying the cosmetic composition to skin.

The chitosan and propolis extract mixture according to the present invention is excellent in skin soothing, moisturizing effect and skin aging prevention effect.

The cosmetic composition comprising the chitosan and propolis extract according to the present invention inhibits the biosynthesis of IL-1α, an inflammatory cytokine, and is excellent in cell stimulation relaxation effect. In the cosmetic composition containing the same, Can be expected to relieve skin irritation and soothe. To induce skin irritation, various cytokines that mediate external stimuli are produced. IL-1α is a typical component. By modulating the production of these ingredients, skin irritation can be mitigated.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Production Example 1

A total of 500 g of the dried chitosan (purchased from DAE-BONG LS) and propolis (purchased from Natural Solutions Co., Ltd.) at a ratio of 1: 1 (dry weight ratio) were collected and put into 2 kg of water, extracted at 95 to 100 ° C. for 2 hours, And dried to obtain 64 g of hot water extract.

Production Example 2

A total of 500 g of the completely dried chitosan (purchased from Daebong LS) and propolis (purchased from Natural Solutions Co., Ltd.) in a ratio of 1: 1 (dry weight ratio) were collected and added to 2 kg of ethanol and extracted at 95 to 100 ° C for 2 hours, And dried to obtain 57 g of an ethanol extract.

Comparative Production Example 1

500 g of completely dried chitosan (purchased from Daebong LS) was collected, put into 2 kg of water, extracted at 95 to 100 ° C for 2 hours, filtered and dried to obtain 63 g of hot water extract.

compare Manufacturing example  2.

500 g of completely dried chitosan (purchased from Daebong LS) was collected and added to 2 kg of ethanol, followed by extraction at 95 to 100 ° C for 2 hours, followed by filtration and drying to obtain 57 g of an ethanol extract.

compare Manufacturing example  3.

500 g of completely dried propolis (purchased from Natural Solutions) was collected and put in 2 kg of water, extracted at 95 to 100 ° C for 2 hours, filtered and dried to obtain 61 g of hot water extract.

compare Manufacturing example  4.

500 g of completely dried propolis (obtained from Natural Solutions Co.) was collected and added to 2 kg of ethanol, followed by extraction at 95 to 100 ° C for 2 hours, followed by filtration and drying to obtain 56 g of an ethanol extract.

Experimental Example  1: Enhancement of Collagen Biosynthesis of Chitosan and Propolis Extracts

Normal human fibroblasts were inoculated into each well of a 48-well microplate at 1 × 10 ⁶ cells and cultured in DMEM (Dulbecco's Modified Eagle Medium) medium at 37 ° C. for 24 hours. Then, in Comparative Experiments 1 to 4 and Preparation Examples 1 and 2, the test group was changed to DMEM medium without serum at a final concentration of 1.0%, and the control group was replaced with serum-free DMEM medium containing no chitosan or propolis extract Were further cultured for 24 hours. After culturing, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured using a kit (Takara, Japan). The amount of PICP was converted to ng / ml, and 250 uM of ascorbic acid was used as a positive control. The increase rate of collagen biosynthesis was calculated according to the following formula (1), and the results are shown in Table 1.

division Increase in collagen biosynthesis (%) Positive control group 75.3 Comparative Preparation Example 1 23.2 Comparative Production Example 2 25.1 Comparative Production Example 3 32.1 Comparative Production Example 4 33.5 Production Example 1 87.3 Production Example 2 86.5

Through the above Experimental Example 1, the chitosan and propolis extracts of Preparation Examples 1 and 2 of the present invention were superior to the single extract (Comparative Preparation Example 1-4) for enhancing collagen biosynthesis. In addition, the effect was superior to ascorbic acid, a positive control.

Experimental Example  2: Effect of chitosan and propolis extract on fibroblast proliferation

Normal human fibroblasts were inoculated into each well of a 96-well microplate to a concentration of 1 x 10 ⁴ cells and cultured in DMEM medium at 37 ° C for 24 hours. Then, the control group, which was replaced with serum-free DMEM medium and the serum-free DMEM medium without chitosan and propolis extract, was changed to a control group of 24 Lt; / RTI > After incubation, MTT (Sigma, USA) solution (3 mg / ml) was added to compare the cell survival rate, and the cell viability was measured at 570 nm using an ELISA reader (Molecular Devices, The fibroblast growth rate (%) was calculated, and the experimental results are shown in Table 2 below. At this time, TGF-β 10 ng / ml was used as a positive control.

division Fibroblast Growth Rate (%) Positive control group 173.2 Comparative Preparation Example 1 112.3 Comparative Production Example 2 118.5 Comparative Production Example 3 121.1 Comparative Production Example 4 122.4 Production Example 1 188.5 Production Example 2 189.3

As can be seen from the results of Table 2, the chitosan and propolis extracts of Preparation Examples 1 and 2 of the present invention were superior to the single extracts (Comparative Preparations 1 to 4) in promoting cell growth. In addition, it was found that the cell growth rate was better than that of the positive control TGF-β.

Experimental Example  3: Effect of chitosan and propolis extract on cell stimulation by lactic acid

Human fibroblasts (Korean Cell Line Bank, Korea) were cultured in T-75 flasks (Falcon, USA) until they grew to about 80%. The cells were transferred to a 96-well plate (Falcon, USA) at 3 × 10 ⁴ cells / well and cultured for 24 hours. After culturing, cells were completely adhered to each other through a microscope to confirm whether they were growing well. Experiments were conducted using lactic acid as a skin cell stimulating agent. At this time, 0.2% of lactic acid was used. That is, 200 占 퐇 of DMEM (Sigma, USA) medium containing 0.2% lactic acid and DMEM medium containing no lactic acid were added to each of the 96-wells, and Comparative Production Examples 1-4 and Preparation Examples 1 and 2 were added to 1.0% w / w). At this time, no lactic acid, chitosan and propolis extract were added to the control group, and only lactic acid was added to the control group. MTT (Sigma, USA) solution (3 mg / ml) was added to compare cell survival rates after 12 hours of addition of the test substance, and the cell viability was measured at 570 nm using an ELISA reader (Molecular Devices, And cell viability (%) was calculated according to the following equation (3). The experimental results are shown in Table 3 below.

division Fibroblast proliferation rate (%) Control group 97.8 Comparative control group (lactic acid 0.2%) 0.0 Comparative Preparation Example 1 (1%) 96.8 Comparative Preparation Example 1 (1%) + lactic acid 0.2% 54.5 Comparative Production Example 2 (1%) 98.2 Comparative Preparation Example 2 (1%) + lactic acid 0.2% 57.9 Comparative Preparation Example 3 (1%) 99.2 Comparative Preparation Example 3 (1%) + lactic acid 0.2% 63.1 Comparative Preparation Example 4 (1%) 98.6 Comparative Preparation Example 4 (1%) + lactic acid 0.2% 64.3 Production Example 1 (1%) 98.9 Production Example 1 (1%) + lactic acid 0.2% 77.6 Production Example 2 (1%) 100.0 Production Example 2 (1%) + lactic acid 0.2% 76.4

As can be seen from the results of Table 3, the experimental results of Production Example 1-2 in which the chitosan and propolis extract of the present invention were treated with and treated with lactic acid showed that the single extract (Comparative Preparation Examples 1-4) And the effect of mitigation of cell stimulation was better than that of control.

Hereinafter, a cosmetic composition containing the chitosan and propolis extract of the present invention will be presented based on the results of the above experimental examples. However, the composition of the present invention is not intended to be limited to the following formulations.

Prescription Example 1: Softening Longevity

Examples of prescription of softening water in cosmetics containing chitosan and propolis extract are as follows.

ingredient content(%) Production Example 2 0.5 glycerin 5.0 1,3-butylene glycol 3.0 Allantoin 0.1 DL-Panthenol 0.3 Iditti-2Na 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 Nicole HCO 60 0.4 Phenoxyethanol 0.3 incense 0.01 Distilled water Balance Sum 100

Prescription example  2: Nourishing lotion

Examples of prescription of nutritional lotion among cosmetics containing chitosan and propolis extract are as follows.

ingredient Content (unit:% by weight) Production Example 2 2.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoin 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Glyoxylic vinyl polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaruronate 5.0 Triethanolamine 0.12 Phenoxyethanol 0.2 incense 0.02 Distilled water Balance Sum 100

Prescription example  3: Nourishing cream

Examples of prescription of nutritional cream among cosmetics containing chitosan and propolis extract are as follows.

ingredient Content (unit:% by weight) Production Example 2 3.0 Pro-type glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Phenoxyethanol 0.2 incense 0.05 Distilled water Balance Sum 100

Comparative Prescription Example  : Nourishing cream

An example of the formulation of the nutritional cream in the cosmetic containing the chitosan ethanol extract (Comparative Preparation Example 3) is as follows.

ingredient Content (unit:% by weight) Comparative Production Example 3 3.0 Pro-type glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaluronate 4.0 Phenoxyethanol 0.2 incense 0.05 Distilled water Balance Sum 100

Experimental Example  4 : HaCaT  Keratinocytes ( keratinocyte ) Inhibited the secretion of IL-1α

The HaCaT keratinocytes (German Cancer Research Institute, Germany) in a 24-well plate 2 × 10 with a density of ⁵ cells / well 10% by FBS is inoculated with DMEM (Dulbeco's Modified Eagle's Medium , Gibco) was added wet screen 37 ℃ , And cultured for 1 day in a 5% CO2 incubator. After exchanging with serum-free DMEM, the samples of Comparative Production Examples 1-4 and Preparation Examples 1 and 2 were all treated at 100 ppm, cultured for 1 day, treated with SDS (sodium dodecyl sulfate, Sigma) Respectively. After 4 hours, a portion of the culture was taken and used for IL-1α quantification and protein quantification. The quantification of IL-1α was performed according to the kit's IL-1α assay using Endogen's human IL-1α kit. The protein was quantitated by the BCA method of Sigma, and the quantification of IL-1α The amount of IL-1? Secreted per protein was measured. As a positive control, Ketopropen, which has been used as a skin irritation mitigating agent, was used. The final IL-1α production rate (%) was calculated by the following formula (4).

division IL-1? Production rate (%) Control group 42.47 ± 1.88 SDS only 100.00 Comparative Preparation Example 1 90.87 + - 5.40 Comparative Production Example 2 91.50 + - 4.80 Comparative Production Example 3 88.22 + - 4.90 Comparative Production Example 4 87.32 ± 3.80 Production Example 1 80.33 + - 2.66 Production Example 2 79.22 + - 3.50

Experimental Example  5: Containing chitosan and propolis extract Cosmetic  Moisture evaluation

The moisturizing effect of the cosmetic of the present invention was evaluated through practical use tests. The nutritional cream of Preparation Example 3 containing 3% (w / w) of chitosan and propolis extract of Preparation Example 2 and the cream of Preparation Example 3 replacing the chitosan and propolis extract with the chitosan ethanol extract of Comparative Preparation Example 3 Was used as a control. A 30-year-old woman applied a square-shaped product to the upper bamboo to apply each product. After applying for 2 weeks, the skin moisturizing amount was changed to a skin corneometer (C + K courage, Germany) The number of subjects improved by 10% or more after the measurement was shown in Table 9, and the skin moisturization improvement ratio was calculated.

cosmetics Skin moisturizing effect Effective rate (%) Number of subjects who improved by more than 10% no effect Cream of Prescription Example 3 26 4 86.7 Comparative Prescription Cream 19 11 63.3 Control cream 11 19 36.7

As can be seen from the results of the experiment shown in Table 9, the cream of Formulation Example 3 of the present invention containing chitosan and propolis extract had a comparative formulation containing a single extract and a control formulation containing purified water instead of chitosan and propolis extract Showed a very high skin moisturizing effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (5)

Chitosan and propolis are mixed in a dry weight ratio of 1: 3 to 3: 1, and water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol Wherein the cosmetic composition has an effect of promoting collagen biosynthesis. The method according to claim 1,
Wherein the mixed extract is contained in an amount of 0.0001 to 30.0% (w / w) based on the total weight of the cosmetic composition. The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Wherein the composition is a cosmetic composition for preventing skin aging. delete delete