KR102378103B1 - Cosmetic Composition Comprising Fermented Extract of Morinda citrifolia and Maca Root as Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fermented extract of noni and maca root as an active ingredient.

Description

Cosmetic Composition Comprising Fermented Extract of Morinda citrifolia and Maca Root as Active Ingredient

The present invention relates to a cosmetic composition comprising, as an active ingredient, a fermented extract of noni and maca root fermented with yeast (Saccharomyces), and more particularly, to a cosmetic composition excellent in antioxidant, skin moisturizing and skin barrier improvement effects.

The goal of cosmetics is to maintain beautiful and healthy skin, and to delay the aging of the skin that occurs naturally with aging. In general, aging is caused by oxidative damage to biological materials by free radicals. That is, highly reactive free radicals peroxidize lipids constituting cell membranes, destroy cell membranes, oxidize proteins, and attack nucleic acids to cause oxidative damage, destroying biomaterials and ultimately causing cell death. The most significant skin characteristics due to aging include wrinkles, loss of elasticity, and dullness of the skin. In addition, in addition to wrinkles and decreased elasticity, after the age of 40, the color of the face is not clear and often dull and dark. When the metabolic ability decreases and the skin becomes rough, the moisture content is also easily reduced. In general, the moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer and becomes about 10 to 30% in the keratinocyte layer. It is known that a substance with hydrophilic water retention ability called Natural Moisturizing Factor (NMF) exists in the keratinocyte layer and plays an important role in moisturizing the skin. When the normal keratinocyte layer has about 10 to 30% moisture, the skin is smooth and soft, and the body's protective function is normally exhibited.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important to prevent skin aging, but other natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate (Sodium lactate), urea, etc. There is a problem of impairing the emulsion stability of this strong cosmetic. Although amino acids, collagen, and elastin have moisturizing properties, there is a limit to their moisturizing ability. The ability to retain moisture in the stratum corneum can be controlled by the natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. The development of substances with high moisturizing effect is one of the important research tasks in cosmetics. In addition, substances known to have antioxidant activity include beta-carotene (β-carotene), dibutylhydroxytoluene (BHT), etc. However, since these substances are used as synthetic products, there is a problem of skin safety when used for a long time or in large amounts. Therefore, there is an urgent need for the development of a new antioxidant cosmetic that can be applied to the skin more safely and usefully.

Therefore, research to develop substances with excellent safety and excellent skin-beauty effects by searching the skin physiological activities of various natural plant components for this purpose is currently being actively conducted.

Korean Patent No. 10-0844275 discloses a cosmetic composition having excellent skin wrinkle improvement and skin elasticity improvement effects including noni extract.

In addition, Korean Patent Registration No. 10-1319999 discloses a cosmetic composition containing cheonmundong and maca fermented extract, which has skin regeneration, soothing and irritation relief effects.

It is an object of the present invention to reduce the risk of problems with existing cosmetic cosmetics and to increase the content of useful active ingredients present in trace amounts in natural substances to provide various physiologically active effects such as antioxidant effect, moisturizing effect or skin elasticity on the skin. It is to provide a cosmetic composition.

The above object is achieved by a cosmetic composition for antioxidant or skin moisturizing containing a fermented extract of noni and maca root as an active ingredient.

Preferably, the cosmetic composition has an effect of improving the skin barrier.

Preferably, the fermented extract of noni and maca root can be obtained by fermenting noni and maca with yeast (Saccharomyces) for 1 to 7 days while maintaining pH 5 to 7 under a temperature condition of 20° C. to 40° C.

Also preferably, the fermented extract of noni and maca root is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. It has any one formulation selected.

The cosmetic composition according to the present invention contains an active ingredient fermented with yeast (Saccharomyces) from noni and maca root, a natural extract that is harmless to the human body and has excellent safety, and has excellent antioxidant, moisturizing, and skin barrier improvement effects.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" means 30, 25, 20, 25, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include, but are not limited to, a given step or element, or group of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present inventors studied the applicability of noni and maca root as a cosmetic containing an active ingredient fermented with yeast (Saccharomyces) among various natural products and measured the antioxidant, moisturizing, and skin barrier improvement effects through various experiments. was found to be expected. As such, a cosmetic composition having an excellent effect was developed by obtaining a component having excellent efficacy in the highest possible yield.

The present invention relates to a cosmetic composition comprising, as an active ingredient, a fermented extract of noni and maca root fermented with yeast (Saccharomyces), and more particularly, to a cosmetic composition excellent in antioxidant, skin moisturizing and skin barrier improvement effects.

Noni ( Morinda citrifolia ) is a plant belonging to the gentian family, and its fruits, leaves, roots, stems, seeds, etc. have been used medicinally in various regions including China. According to the ancient literature of the South Pacific region, it is recorded as the best natural remedy. Noni has been proven to be safe and is widely used around the world to treat various diseases. In particular, as it contains a large amount of polyphenols, it has high antioxidant efficacy and is highly evaluated for preventing skin aging.

The noni extract of the present invention means a product obtained by extraction from various organs or parts of noni (eg leaves, branches, flowers, roots, stems, fruits, etc.), preferably an extract obtained from a plant or fruit, and most preferably a fruit extracts obtained from

On the other hand, the noni extract of the present invention can be prepared using a conventional solvent under conditions of temperature and pressure according to a conventional method known in the art. That is, the noni extract contains water, anhydrous or hydrous lower alcohol having 1 to 5 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane. And extraction using a solvent selected from the group consisting of and mixtures thereof, or extracting noni using the solvent, and then mixing them may be prepared, but preferably the solvent is alcohol, more preferably 70% ethanol It is better to use And the suitable amount of the extraction solvent is 1 to 15 times the dry weight of noni, more preferably 5 to 10 times, and most preferably 6 times.

Maca ( Lepidium meyenii ) is a biennial herb of the Chinese cabbage family, and the root is mainly used for medicinal purposes. Maca, which is called the wild ginseng of the Andes and attracts attention as a superfood, is a radish-like plant and comes in three colors: yellow, red, and black. Maca is a plant that grows wild in the Andean Plateau at an altitude of 4000 meters above sea level in Peru. It is rich in various minerals and essential amino acids. It contains various active ingredients to maintain life by surviving in harsh alpine conditions where oxygen is scarce, and these ingredients are known to be beneficial to human health. Maca contains large amounts of proin and arginine amino acids, which are major components of collagen. This increases collagen synthesis in the skin to maintain skin elasticity and prevent aging.

According to one embodiment of the present invention, the fermentation extract of Noni and Maca is fermented with yeast (Saccharomyces) for 1 to 7 days while maintaining pH 5 to 7 under a temperature condition of 20 to 40 ° C. It means obtained by extraction using a solvent.

According to a preferred embodiment of the present invention, the content of the active ingredient obtained by fermenting noni and maca root raw material added to the cosmetic composition with yeast (Saccharomyces) is 0.0001 to 30.0% by weight, preferably 0.001 to 30.0% by weight, based on the total weight of the cosmetic composition. 25.0% by weight, most preferably 0.1 to 10.0% by weight. When the content of the active ingredient fermented by yeast (Saccharomyces) of the noni and maca root raw materials is less than 0.0001 wt%, antioxidant, moisturizing, and skin barrier improvement effects do not appear, and when it exceeds 30 wt%, the effect on increasing the content increases The degree is insignificant, there is a problem in safety and stability of the formulation, and it is not economical either.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation and spray, etc., and more specifically, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, It can be prepared in the form of cleansing water, pack, spray or powder. And the ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions in addition to the active ingredient as an active ingredient, for example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers includes

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like may be used.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the present invention will be described in more detail through a preparation example. These preparation examples are only for explaining the present invention in more detail, and it is to those of ordinary skill in the art to which the present invention belongs that the scope of the present invention is not limited by these preparation examples according to the gist of the present invention. it will be self-evident

<Preparation Example 1> Preparation of noni extract

After washing the noni fruit with purified water, drying it, and pulverizing it, it was made fine using a 100 mesh sieve. 70% ethanol was added to the noni pulverized product to 100 g/L, extracted under reflux three times for 5 hours each, and cooled, filtered with Whatman #3 filter paper, concentrated under reduced pressure at 50° C. or less, and freeze-dried. The freeze-dried product was dissolved to 2% (W/V) using 50% glycerin to prepare a noni extract (Preparation Example 1).

<Preparation Example 2> Preparation of maca root extract

The maca roots were washed with purified water, dried, and then all pulverized and made fine using a 100 mesh sieve. 70% ethanol was added to the noni pulverized product to 100 g/L, extracted under reflux three times for 5 hours each, and cooled, filtered with Whatman #3 filter paper, concentrated under reduced pressure at 50° C. or less, and freeze-dried. The freeze-dried product was dissolved to 2% (W/V) using 50% glycerin to prepare a maca root extract (Preparation Example 2).

<Preparation Example 3> Preparation of fermented extract of noni and maca root

It was prepared as an extract by fermenting noni fruit and maca root, and after selecting noni and maca root as a sample, washing them sufficiently, and making the pulverized product fine so that it can pass through a 300 mesh sieve. This was added to the culture medium of the Saccharomyces genus (Saccharomyces cerevisiae KCTC 7904) strain of noni and maca root to 20g/L. Here, 2% (W/V) of glucose was added as a carbon source, and 0.5% (W/V) of peptone was additionally added and cultured. The culture was carried out using a 5L fermenter at 37°C, preferably at pH 6 while maintaining at pH 5-7, and preferably for 1 to 7 days, preferably for 5 days. After culturing, the culture medium was centrifuged to remove the culture medium.

The fermented noni and maca root from which the culture was first removed was extracted with ethanol three times for 5 hours each by adding ethanol to make a final 70% (V/V) aqueous solution of ethanol, followed by cooling, followed by cooling with Whatman #2 filter paper was filtered. The filtered extract was transferred to a concentration tank, and the filtered extract was concentrated under reduced pressure and freeze-dried. This powder was dissolved to 2% (W/V) using 50% glycerin to prepare a noni and maca root fermented extract (Preparation Example 3).

<Formulation Example 1>

Formulation examples of creams among cosmetics containing the fermented extract of noni and maca root (Preparation Example 3) are shown in Table 1 below.

ingredient content (wt%) Fermented extract of noni and maca root
(Production Example 3) 5.0 glycerin 5.0 Sodium Hyaluronate 4.0 Polysorbate 60 1.5 mineral oil 5.0 squalane 3.0 dimethicone 2.0 triethanolamine 1.0 Lipophilic monostearic glycerin 2.0 cetearyl alcohol 2.2 Stearic Acid 1.5 Sorbitan Stearate 0.6 Sodium Magnesium Silicate 0.1 betaine 3.0 antiseptic a very small amount incense a very small amount pigment a very small amount Purified water remaining amount total 100

<Comparative Formulation Example 1>

Comparative Formulation Example 1 of the cream not containing the fermented extract of noni and maca root is shown in Table 2 below.

ingredient content (wt%) glycerin 5.0 Sodium Hyaluronate 4.0 Polysorbate 60 1.5 mineral oil 5.0 squalane 3.0 dimethicone 2.0 triethanolamine 1.0 Lipophilic monostearic glycerin 2.0 cetearyl alcohol 2.2 Stearic Acid 1.5 Sorbitan Stearate 0.6 Sodium Magnesium Silicate 0.1 betaine 3.0 antiseptic a very small amount incense a very small amount pigment a very small amount Purified water remaining amount total 100

<Comparative Formulation Example 2>

Comparative Formulation Example 2 of a cream in a cosmetic containing a noni extract (Preparation Example 1) is shown in Table 3 below.

ingredient content (wt%) Noni extract (Preparation Example 1) 5.0 Sodium Hyaluronate 4.0 Polysorbate 60 1.5 mineral oil 5.0 squalane 3.0 dimethicone 2.0 triethanolamine 1.0 Lipophilic monostearic glycerin 2.0 cetearyl alcohol 2.2 Stearic Acid 1.5 Sorbitan Stearate 0.6 Sodium Magnesium Silicate 0.1 betaine 3.0 antiseptic a very small amount incense a very small amount pigment a very small amount Purified water remaining amount total 100

<Comparative Formulation Example 3>

Comparative Formulation Example 3 of cream among cosmetics containing maca root extract (Preparation Example 2) is shown in Table 4 below.

ingredient content (wt%) Maca root extract (Preparation Example 2) 5.0 Sodium Hyaluronate 4.0 Polysorbate 60 1.5 mineral oil 5.0 squalane 3.0 dimethicone 2.0 triethanolamine 1.0 Lipophilic monostearic glycerin 2.0 cetearyl alcohol 2.2 Stearic Acid 1.5 Sorbitan Stearate 0.6 Sodium Magnesium Silicate 0.1 betaine 3.0 antiseptic a very small amount incense a very small amount pigment a very small amount Purified water remaining amount total 100

<Experimental Example 1> Measurement of antioxidant effect

Antioxidant activity of the extracts prepared in Preparation Examples 1 to 3 of the present invention was measured by thiobarbituric acid method (TBA assay) using a lipid peroxidation system by Fenton reaction. That is, in 5 ml of 25 mM Trizma hydrochloric acid/0.75 mM potassium chloride buffer containing 10 μl of ethyl linoleate, 0.2% sodium dodecyl sulfoxide (SDS), 10 μmol of ferrous chloride, and 2 μmol of hydrogen peroxide, prepared in Preparation Examples 1 to 3 After adding a test solution of dimethyl sulfoxide (DMSO) to the extract, incubated at 55° C. for 16 hours, 50 μl of 4% dibutylhydroxyl toluene (BHT) was added to stop the reaction. Put 0.3 ml of this solution in a test tube, add 3.0 ml of 0.05N hydrochloric acid and 0.67% thiobarbituric acid (TBA), and boil for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer was measured at 535 nm after centrifugation. The antioxidant effect of each extract was calculated by Equation 1 below. The results are shown in Table 7.

test substance Antioxidative effect(%) experimental concentration
(10㎍/ml) experimental concentration
(50㎍/ml) experimental concentration
(100㎍/ml) Preparation Example 1 23 44 61 Preparation Example 2 27 51 63 Preparation 3 41 68 87

Most of the antioxidant effects of the noni and maca root extracts of Preparation Examples 1 to 3 showed high activity, and in particular, it can be seen that the antioxidant effect of Preparation Example 3 according to the present invention was very excellent.

In addition, the noni maca root extracts of Preparation Examples 1 to 3 and the existing antioxidants ascorbic acid, dl-α-tocopherol, beta-carotene (β-carotene) and dibutylhydroxyltoluene The antioxidant activity of (BHT) was measured in the same manner as in Experimental Example 2, and IC ₅₀ , which was a 50% inhibitory concentration, was compared. The results are shown in Table 8 below.

test substance IC ₅₀ (10 μg/ml) Preparation Example 1 32.1 Preparation Example 2 34.2 Preparation 3 52.1 ascorbic acid 21.91 dl-a-tocopherol (dl-a-tocopherol) 3.36 Beta-carotene (β-carotene) 29.8 Dibutylhydroxyltoluene (BHT) <1

The noni and maca root extracts of Preparation Examples 1 to 3 exhibited high activity comparable to other antioxidants, and in particular, it can be seen that Preparation Example 3 exhibited high activity. In particular, it can be seen that Preparation Example 3 has significant antioxidant activity despite being a natural product mixture.

<Experimental Example 2> Evaluation of skin moisturizing improvement effect

The anti-wrinkle effect of the cream containing the noniwa maca root ferment extract (Formulation Example 1) and the cream of Comparative Formulation Examples 1 to 3 was evaluated as follows. For 40 people in their 20s and 40s without skin disease, each group was divided into two groups of 20 people each, and in each group, the nourishing cream of the formulation example 1 and Comparative Formula Examples 1 to 3 was applied twice daily to the face and face for 1 month. It was applied to the forearm. Before starting application, the skin conductivity was measured using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under constant temperature and humidity conditions (24℃, 40% humidity) before starting the application, and the basic value was taken, and after 1 week, 2 weeks, and 4 weeks, The increase rate was evaluated by measuring the skin conductivity increase rate (%). The results are shown in Table 9 below.

experimental product 1 week later After two weeks after 3 weeks Formulation Example 1 46 49 51 Comparative Formulation Example 1 21 18 17 Comparative Formulation Example 2 26 30 35 Comparative Formulation Example 3 19 25 31

As shown in Table 9 above, in the case of the cream (Formulation Example 1) containing the fermented noni and maca root extract, the skin conductivity increase rate was very excellent compared to Comparative Formulation Examples 1 to 3. In general, since skin conductivity is proportional to the amount of skin moisture, the above results confirm that cosmetics containing noni and maca root fermented extract maintain high skin moisture content compared to cosmetics that do not. Skin irritation was not observed in most subjects who applied this cosmetic to the skin.

<Experimental Example 4> Evaluation of skin barrier improvement effect

The noni and maca root fermented extract of the present invention In order to find out the skin barrier improvement effect of the cosmetic containing it, the experiment was conducted as follows. In this experimental example, the amount of Trans-Epidemal Water Loss (TEWL) for the cosmetic of Formulation Example 1 was evaluated by performing a comparative experiment using a Tewameterer (TM300, Courage and Khazaka Electronic Co., Germany). For 40 women in their 20s and 40s, apply each sample to the right and left faces, and measure the amount of transdermal moisture loss for 1 hour, 2 hours, 4 hours, and 6 hours before and after application to the area 3 cm to the right and 1 cm below the eye area. It was measured three times using a Tewameterr (TM300, Courage and Khazaka Electronic Co., Germany) and the average value was used to evaluate the amount of transdermal water loss. The results are shown in Table 10 below.

division 1 hours 2 hours 4 hours 6 hours Formulation Example 1 14.0 13.1 11.3 8.2 Comparative Formulation Example 1 17.9 16.7 13.6 11.4 Comparative Formulation Example 2 18.9 17.3 15.2 14.2

As shown in Table 10, as a result of measuring the amount of transdermal moisture loss, it was found that the amount of transdermal moisture loss was decreased in the facial skin of the experimenter who applied the cream containing the noniwa maca root yeast (Saccharomyces) ferment extract. It was found to have a better effect.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

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Claims (6)

A cosmetic composition for moisturizing the skin containing a fermented extract of noni fruit and maca root as an active ingredient,
The fermented extract of noni fruit and maca root is obtained by fermenting noni fruit and maca root with yeast (Saccharomyces) for 1 to 7 days while maintaining pH 5 to 7 under a temperature condition of 20 ° C to 40 ° C. cosmetic composition for According to claim 1, wherein the cosmetic composition has a skin barrier improvement effect, the skin moisturizing cosmetic composition. The cosmetic composition for moisturizing the skin according to claim 1, wherein the fermented extract of noni fruit and maca root is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition of claim 1, wherein the cosmetic composition is composed of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. A cosmetic composition for skin moisturizing, having any one formulation selected from the group. delete delete