KR102567305B1 - Cosmetic composition for pore shrinking and sebum control containing natural ingredients - Google Patents

Abstract

The present invention relates to a cosmetic composition containing natural complex extract as an active ingredient, and more specifically, to a cosmetic composition that contains complex extracts of green apple, green coffee bean, green tomato, and sabal palm fruit as active ingredients and exhibits excellent antioxidant, pore shrinking (pore reducing), and sebum secretion suppressing effects.

Description

Cosmetic composition for pore shrinking and sebum control containing natural ingredients containing natural complex extracts as active ingredients {Cosmetic composition for pore shrinking and sebum control containing natural ingredients}

The present invention relates to a cosmetic composition containing a natural complex extract as an active ingredient, and more particularly, contains a green apple, green coffee bean, green tomato and saba palm fruit complex extract as an active ingredient to provide excellent antioxidant and pore contraction (convergence) And it relates to a cosmetic composition exhibiting a sebum secretion inhibitory effect.

In general, the production of sebum in the skin, including the scalp and face, plays a role in maintaining moisture in the skin and preventing the invasion of microorganisms, but excessive secretion of sebum causes shine of the skin, lifting of makeup, enlargement of pores and aging, and post-puberty. It can also trigger the formation of acne. Various causes are involved in the excessive secretion of sebum, and among them, the cause caused by the activation of sebaceous gland cells by the action of hormones is known to be the most important factor. Accordingly, female hormones such as estrogen have been used to treat excessive secretion of sebum or acne caused by male hormones in the past, but due to skin inflammation and side effects caused by hormone administration, the use is currently discontinued or a very small amount is used. The situation is. Currently, as a method of suppressing sebum secretion in cosmetics, there are methods of using porous powder that temporarily absorbs sebum or using extracts known to inhibit sebum, but the effect is insignificant due to the small amount of active ingredients. is about

The present inventors conceived that the core of pore care is the inhibition of excessive sebum production due to hormone action and the contraction of enlarged pores, so that they contracted skin pores through astringent action with a natural substance that inhibits the activity of 5α-reductase, an enzyme that promotes sebum production. Research has been conducted to develop cosmetics for natural pore reduction and sebum control using natural substances that help, and the complex extracts of green apple, green coffee bean, green tomato and sabal palm fruit are excellent in inhibiting the activity of 5α-reductase that promotes sebum production, The present invention was completed by confirming that the pore shrinking effect was exhibited by excellent astringent action.

(001) Republic of Korea Patent No. 10-1194767 (2012.10.19) (002) Republic of Korea Patent No. 10-1628939 (2016.06.02)

An object of the present invention is to provide a cosmetic composition containing a complex extract of green apple, green coffee bean, green tomato and saba palm fruit as an active ingredient and exhibiting excellent antioxidant effect, sebum secretion inhibition and pore contraction effect.

According to the present invention in order to achieve the above object, there is provided a cosmetic composition containing green apple, green coffee bean, green tomato and fruit complex extract of sabal palm as an active ingredient.

Preferably, the composite extract is characterized in that it is a mixture of extracts extracted from green apple, green coffee bean, green tomato and sabal palm fruit at a low temperature of 20 to 25 ° C and a high temperature of 90 to 100 ° C, respectively. The composite extract is made by mixing the low-temperature extract and the high-temperature extract in a weight ratio of 1 to 3:1 to 3, respectively.

The composite extract is characterized in that green apple, green coffee bean, green tomato and sabal palm fruit are mixed and extracted in a weight ratio of 0.5 to 1:0.5 to 1:0.5 to 1:0.5 to 1, respectively.

As an active ingredient, the composite extract is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is characterized in that it is for antioxidant, sebum secretion inhibition or pore contraction.

The natural complex extract of green apple, green coffee bean, green tomato, and sabal palm fruit of the present invention is safe as a natural extract, and exhibits excellent antioxidant effect, sebum secretion inhibition, and pore contraction (convergence) effect due to its synergistic effect, so it can be used as a cosmetic can be useful

1 is a graph showing the sebum secretion control effect of the composite extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The technical feature of the present invention is that the composite extract of green apple, green coffee bean, green tomato and saba palm fruit exhibits excellent antioxidant effect, pore contraction effect and sebum secretion control effect due to its synergistic effect.

A green apple means an apple that is green before ripening to red. Green apples contain about 10 times more polyphenols, which are antioxidants, than regular apples, so they have excellent anti-aging effects by removing active oxygen, the main culprit of aging. It has been found that applephenone, a polyphenol derived from green apples, protects and soothes the skin by suppressing the production of inflammatory factor NO, and suppresses SREBP 1, a protein involved in sebum production.

Green coffee beans refer to green coffee beans that have not been roasted, and green coffee beans contain beneficial compounds such as chlorogenic acid, diterpene, and melanoidin, as well as caffeine, a powerful antioxidant. Caffeine is a protective ingredient that acts as an insecticide that kills pests that feed on plants. It helps improve skin inflammation by reducing the production of inflammatory mediators in the body and shrinks pores through astringent action.

Green tomatoes with a green light contain tomatidine, which inhibits a protein that causes muscle aging. Tomatidine helps to synthesize collagen around the pores and improves sagging pores, especially the effect of improving vertical pores. In addition, in the case of heat-treated green tomatoes, the content of polyphenols and flavonoids increases, helping to improve the pores.

Sabal palm fruit was used as a food or medicine to treat diseases of the genitourinary system of Native American men early in the 18th century, and it grows in a dry environment and contains various active ingredients to help with anti-inflammatory and soothing. It is effective for inflammatory diseases by inhibiting the activity of 5α-Reductase, which is the cause of sebum secretion.

According to the present invention, there is provided a cosmetic composition containing a complex extract of green apple, green coffee bean, green tomato and fruit of sabal palm as an active ingredient.

In the preparation of the composite extract, selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether At least one solvent may be used.

More preferably, the complex extract is characterized in that it is a mixture of extracts obtained by extracting green apple, green coffee bean, green tomato, and fruit of sabal palm at a low temperature of 20 to 25 ° C and a high temperature of 90 to 100 ° C, respectively. The composite extract is made by mixing the low-temperature extract and the high-temperature extract in a weight ratio of 1 to 3:1 to 3, respectively.

According to a preferred embodiment of the present invention, the composite extract is (A) green apple, green coffee bean, green tomato, and 20 to 24 hours at a low temperature, preferably 20 to 25 ℃ by adding purified water to the palm fruit mixture Extracting to prepare a low-temperature extract; (B) preparing a high-temperature extract by adding purified water to a mixture of green apple, green coffee bean, green tomato and saba palm fruit and extracting at a high temperature, preferably at a temperature of 90 to 100 ° C. for 2 to 3 hours; and (C) mixing the two extracts. Compared to each of the low-temperature extract and the high-temperature extract, the combined extract showed more excellent antioxidant, sebum secretion inhibition, and pore contraction effects.

In the preparation of the extract, green apple, green coffee bean, green tomato and sabal palm fruit are mixed and extracted in a weight ratio of 0.5 to 1:0.5 to 1:0.5 to 1:0.5 to 1, respectively, 1:1: It is more preferable because it shows a more excellent effect when mixed and extracted in a weight ratio of 1: 1.

The composite extract of the present invention thus prepared exhibited excellent antioxidant activity (Test Example 1), sebum secretion inhibitory activity (Test Examples 2 and 3) and pore contraction activity (Test Example 4).

The composite extract as an active ingredient is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may be usefully used for antioxidant, pore contraction, or sebum secretion inhibition.

In addition to the composite extract, the cosmetic composition of the present invention is a cosmetic or dermatological field such as a fatty substance, a surfactant, a preservative, a vitamin, a wetting agent, water, an emulsifier, an essential oil, or any other component commonly used in cosmetics. It may contain commonly used adjuvants. In addition, the above components may be introduced in an amount generally used in the field of skin science.

In addition, the formulation of the cosmetic composition in the present invention is not limited, but skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, essence, It may be applied in any one formulation selected from the group consisting of pack, soap, shampoo, treatment, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, and eye shadow.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are only for illustrating the present invention, and the present invention is not limited by the following examples.

Example 1-2: Preparation of low-temperature natural complex extract

After mixing green apples, green coffee beans, green tomatoes, and Sabal palm fruits in the ratio shown in Table 1 below, they were put in purified water corresponding to 10 times the dry weight and extracted at low temperature (20-25 ° C) for 24 hours. This was concentrated at a temperature of 50 to 60 ° C using a vacuum concentrator and then freeze-dried with a lyophilizer to obtain an extracted powder.

weight ratio green apple green coffee bean green tomato sabal palm fruit Example 1 One 0.5 One 0.5 Example 2 One One One One

Examples 3-4: Preparation of high-temperature natural complex extract

After mixing green apples, green coffee beans, green tomatoes, and Sabal palm fruits in the ratio shown in Table 2 below, they were put in purified water corresponding to 10 times the dry weight and extracted at high temperature (90 ~ 100 ℃) for 2 hours. This was concentrated at a temperature of 50 to 60 ° C using a vacuum concentrator and then freeze-dried with a lyophilizer to obtain an extracted powder.

weight ratio green apple green coffee bean green tomato sabal palm fruit Example 3 One 0.5 One 0.5 Example 4 One One One One

Examples 5-6: Preparation of natural complex extract

The low-temperature composite extract and the high-temperature composite extract of green apple, green coffee bean, green tomato, and saba palm fruit prepared in the above example were mixed in the same weight ratio to Example 5 (Example 1 + Example 3) and Example 6, respectively. (Example 2 + Example 4).

Comparative Example 1: Preparation of low-temperature natural complex extract

After mixing green apples, green coffee beans, and green tomatoes in the ratio shown in Table 3 below, they were put in purified water corresponding to 10 times the dry weight and extracted at low temperature (20-25 ° C) for 24 hours. This was concentrated at a temperature of 50 to 60 ° C using a vacuum concentrator and then freeze-dried with a lyophilizer to obtain an extracted powder.

weight ratio green apple green coffee bean green tomato Comparative Example 1 One One One

Comparative Example 2: Preparation of high-temperature natural complex extract

Green apples, green coffee beans, and green tomatoes were mixed in the ratio shown in Table 4 below, put in purified water corresponding to 10 times the dry weight, and extracted at high temperature (90-100 ° C) for 2 hours. This was concentrated at a temperature of 50 to 60 ° C using a vacuum concentrator and then freeze-dried with a lyophilizer to obtain an extracted powder.

weight ratio green apple green coffee bean green tomato Comparative Example 2 One One One

Comparative Example 3: Preparation of natural complex extract

Comparative Example 3 was obtained by mixing the low-temperature composite extract and the high-temperature composite extract of green apple, green coffee bean, and green tomato prepared in Comparative Examples 1 and 2 in the same weight ratio.

Test Example 1: Antioxidant effect

The DPPH free radical scavenging ability of the extracts according to the comparative examples and examples was measured.

After mixing 0.1 mL of the sample solution of each Example and Comparative Example and 0.1 mL of DPPH (1,1-diphenyl-2-picrylhydrazyl) solution, leave it for 30 minutes, and then evaluate the radical scavenging activity of the sample at 520 nm using an ELISA Reader. did At this time, ascorbic acid was used as a positive control, and DPPH radical scavenging activity was calculated by the following formula. The results are shown in Table 5 below.

DPPH radical scavenging activity (%) = [(control-sample)/control] × 100

sample Concentration (wt/v%) Free radical scavenging activity (%) Comparative Example 1 0.1 59.4 Example 1 0.1 62.2 Example 2 0.1 69.8 Comparative Example 2 0.1 70.3 Example 3 0.1 72.2 Example 4 0.1 75.8 Comparative Example 3 0.1 81.1 Example 5 0.1 83.9 Example 6 0.1 85.4

As shown in Table 5, the composite extract of green apple, green coffee bean, green tomato, and fruit of Sabal palm exhibited better DPPH radical scavenging activity than the green apple, green coffee bean, and green tomato composite extract of Comparative Example, and the low-temperature extract and The composite extracts of Examples 5 and 6 prepared by mixing the high-temperature extracts showed the best DPPH radical scavenging ability, and thus it was confirmed that the antioxidant ability was the best. Ascorbic acid (50ppm), the control group, showed a clearance rate of 86.03%.

Test Example 2: Sebum control efficacy

Cells were stained by staining the neutral lipids (triacylglycerol, cholesterol ester) inside the cells through the Oil Red O staining method, and the sebum control efficacy of each extract was compared with images or after dissolving in isoprotanol, the absorbance was compared.

Specifically, primary sebocytes were dispensed into a 24-well plate at a concentration of 6 × 10⁴cells/well and cultured overnight. In order to promote sebum secretion, linoleic acid (50 μM), arachidonic acid (50 μM), and dihydrotestosterone (50 nM) were treated as stimulants, and each extract prepared in Comparative Examples and Examples was not treated or treated together. After culturing for 4 days, the medium was removed and washed with PBS. Thereafter, 10% paraformaldehyde in PBS was added to fix the cells for 15 minutes, and then the cells were washed again with PBS. Oil Red O staining solution was treated for 15 minutes, washed with EtOH and DIW to remove excess dye, put into PBS for microscopic observation, and after removing PBS, 4% NP-40 in isopropyl alcohol was added to dye the cells. After melting, the absorbance was measured at 520 nm, and the degree of neutral lipid production was compared by % based on the non-stimulated group (extract non-treated group) to evaluate the sebum control effect.

As a result, while the Induction Control group, which is a positive control group, increased the production of neutral lipids, the samples of Comparative Examples and Examples decreased the production of neutral lipids despite the sebum secretion promoting action. In particular, it was confirmed that the examples showed a more excellent sebum inhibitory effect than the comparative example (FIG. 1).

Test Example 3: Sebum secretion inhibition test

The 5α-reductase activity inhibition rate of the extract samples according to the comparative examples and examples was measured.

Adult Sprague-Dawley male rats (7-8 weeks old) fasted for 24 hours were killed with diethyl ether, and then the abdominal prostate was removed and the connective tissue was removed. A buffer solution (0.32M sucrose, 0.1mM dithiothreitol, 20mM sodium acetate) was added thereto, chopped into small pieces, and then suspended using a grinder. The suspension was centrifuged to obtain a supernatant to partially purify 5α-reductase. An activity inhibition experiment was performed using this enzyme.

A portion of the obtained supernatant was placed in a buffer solution containing 0.2M monobasic acid and 0.2M dibasic acid, and then reacted with a substrate (testosterone) to which the isotope 3H was attached to measure the amount of dihydrotestosterone produced. . The reaction solution was prepared by adding 1 mM dithiothreitol, 40 mM sodium phosphate (pH 6.5), 50 μM NADPH, [1,2,6,7-3H] testosterone/testosterone and enzyme suspension (0.5 mg) to a total volume of 640 μl. .

After dissolving the sample to 1% in 10% ethanol, 6.40 μl of the sample was added to the above reaction solution to make the final concentration 0.01%. As a control group, the same volume of solvent was used, and as a positive control group, a well-known substance called finasteride, an inhibitor of 5α-reductase approved by the US FDA, was used.

The reaction was started simultaneously with the addition of the enzyme suspension and proceeded at 37 ° C. for 30 minutes, followed by extraction by adding 1 ml of ethyl acetate, and 100 μl of ethyl acetate was applied to silica plastic sheet kieselgel F254 (Silica plastic sheet kieselgel F254). The developing solvent system was developed with acetate-cyclohexane (1:1).

After drying the plastic sample in the air, a bath system was used to measure the amount of isotope. The elemental content was measured. The results for each inhibition rate are shown in Table 6 below.

* T: 3H radioactivity in testosterone area (Radio Activity)

** DHT: 3H radioactivity in the area of dihydrotestosterone

*** Conversion rate: radioactivity to DHT area / total radioactivity × 100

**** Inhibition rate: ((conversion rate of control group - conversion rate of sample) / conversion rate of control group) × 100

Sample (0.01%) T(dpm) DHT (dpm) Conversion rate (%) Inhibition rate (%) Comparative Example 1 7749 3905 33.50 24.22 Example 1 7967 3611 31.18 29.47 Example 2 8237 3623 30.54 30.92 Comparative Example 2 7246 2946 28.90 34.63 Example 3 7983 2679 25.12 43.18 Example 4 8649 2574 22.93 48.13 Comparative Example 3 8367 1997 19.26 56.43 Example 5 7841 1369 14.86 66.38 Example 6 8249 1164 12.36 72.04 control group 8694 6891 44.21 - positive control 8103 2888 26.27 40.57

As a result, the composite extract of green apple, green coffee bean, green tomato, and sabal palm fruit of Example showed a more excellent 5α-reductase inhibitory effect than the green apple, green coffee bean, and green tomato composite extract of Comparative Example, and The composite extracts of Examples 5 and 6 prepared by mixing the extract and the high-temperature extract exhibited the best 5α-reductase inhibitory effect, and it was confirmed that the sebum control activity was the best.

Test Example 4: Pore contraction effect test

In order to test the pore contraction effect, the hemoglobin precipitation rate was measured using the composite extract samples according to the Comparative Examples and Examples.

The pore contraction effect was confirmed using bovine hemoglobin protein for the samples of the comparative examples and examples. PBS (phosphate bufferedsaline, pH 7.4) was mixed with hemoglobin at a ratio of 1:1 (1 mg/1 ml), centrifuged at 3,000 rpm for 5 minutes, and the supernatant was separated to measure absorbance at 407 nm using an optical instrument. . As a control, 2 ml of the mixed solvent was used instead of the sample. The pore contraction effect was measured by measuring the amount of hemoglobin precipitated when the final concentration of the samples of Comparative Examples and Examples contained in the reaction solution was 1 to 5%, and the results are shown in Table 7. The higher the hemoglobin precipitation rate, the better the pore shrinking effect.

sample / concentration (%) Hemoglobin precipitation (%) 0 0.1 0.5 1.0 5.0 control group 0.1 0.1 0.1 0.1 0.1 Comparative Example 1 0.1 1.2 3.3 8.8 37.8 Example 1 0.1 1.6 4.1 10.5 41.3 Example 2 0.1 2.0 5.0 11.7 49.7 Comparative Example 2 0.1 1.5 7.5 14.2 46.8 Example 3 0.1 2.2 9.4 16.9 50.9 Example 4 0.1 2.7 11.5 18.3 54.5 Comparative Example 3 0.1 3.2 13.8 20.1 61.3 Example 5 0.1 3.6 14.0 28.2 71.4 Example 6 0.1 4.1 16.7 33.6 76.2

As confirmed in Table 7, the composite extracts of Examples 5 and 6 prepared by mixing the low-temperature extract and the high-temperature extract showed a higher precipitation rate, indicating that the pore contraction effect through the protein coagulation effect was excellent.

Test Example 5: Cytotoxicity and Proliferation Experiment

Cytotoxicity and proliferation experiments were conducted in the following manner. Normal human fibroblasts were inoculated into each well of a 96-well microplate to form 1 × 10⁴ cells, and cultured in DMEM medium at 37° C. for 24 hours. Subsequently, the experimental group replaced with DMEM medium containing 2.5% serum at a final concentration of 0.1, 0.5, 1.0, 2.0, and 5.0%, and the control group replaced with serum-free DMEM medium were further cultured for 24 hours. After culturing, in order to compare cell viability, MTT (Sigma, United States) solution (3mg/ml) was added and cell viability was measured using ELISA READER (Molecular Devices, United States) to measure absorbance at 570 nm and optimal conditions for cell growth Simultaneous culture was performed under the same conditions as above, and cell proliferation of the control group was set as 100%, and the cell growth rate of the sample input experimental group was calculated.

Cell proliferation rate (%) = ( OD at 570 nm of sample-added group/ OD at 570 nm of sample-free group) × 100

density(%) Cell proliferation rate (%) control group 0.1 0.5 1.0 2.0 5.0 Comparative Example 1 100 99.4 99.9 100.1 100.0 100.1 Example 1 100 98.9 98.7 101.1 100.6 100.0 Example 2 100 100.0 101.0. 99.4 99.5 100.8 Comparative Example 2 100 98.0 99.5 102.0 100.8 100.4 Example 3 100 100.4 102.1 99.3 100.6 99.7 Example 4 100 100.5 99.9 100.1 99.9 100.9 Comparative Example 3 100 99.2 101.1 100.0 100.3 98.8 Example 5 100 100.1 100.7 100.3 98.7 101.0 Example 6 100 100.0 99.3 101.0 100.4 101.2

As shown in Table 8, when the cell proliferation in the optimal cell growth conditions is 100%, considering the measurement error range, the composite extracts of green apple, green coffee bean, green tomato, and Sabal palm fruit are all non-toxic to cells, It was found that there was a cell proliferation effect.

Example 7: Preparation of Serum Containing Natural Complex Extract

Serum containing the composite extract prepared in Example 6 was prepared in a conventional manner according to the composition of Table 9 below.

ingredient Content (% by weight) Example 7 Complex extract (Example 6) 5.0 carbomer 0.1 Methylgluses-20 2.0 glycereth-26 2.5 jojoba oil 2.0 MCT Oil 1.0 dimethicone 2.0 Citric Acid 0.3 propylene glycol 5.0 Butylene Glycol 3.0 1,2-hexanediol 2.0 allantoin 0.2 betaine 0.2 incense a very small amount Purified water balance

Claims (8)

Green apple, green coffee bean, green tomato and sabal palm fruit are extracted at a low temperature of 20 ~ 25 ℃ and high temperature of 90 ~ 100 ℃. A cosmetic composition containing a. delete The cosmetic composition according to claim 1, wherein the composite extract is formed by mixing the low-temperature extract and the high-temperature extract in a weight ratio of 1 to 3:1 to 3, respectively. The method of claim 1, wherein the composite extract is extracted by mixing green apple, green coffee bean, green tomato and sabal palm fruit in a weight ratio of 0.5 to 1:0.5 to 1:0.5 to 1:0.5 to 1, respectively. A cosmetic composition to do. The cosmetic composition according to claim 1, wherein the composite extract as an active ingredient is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the cosmetic composition is for antioxidation. The cosmetic composition according to claim 1, wherein the cosmetic composition is for inhibiting sebum secretion. The cosmetic composition according to claim 1, wherein the cosmetic composition is for shrinking pores.