KR101921832B1 - Cosmetic Compositions for Skin Whitening Containing Ginseng Steaming Extract - Google Patents

Abstract

The present invention relates to a method for preparing a fraction of a steamed ginseng liquid, and since a fraction obtained by treating the steamed ginseng liquid with an aqueous solution of 3 to 8 (v/v)% of a NaOH aqueous solution and fractionating the steamed ginseng liquid with butanol has an excellent skin whitening effect, a preparation method of the present invention or the fraction of the steamed ginseng liquid can be usefully used in the field of cosmetics requiring the skin whitening effect.

Description

[0001] The present invention relates to a cosmetic composition for skin whitening,

The present invention relates to a cosmetic composition for skin whitening comprising a rosemary solution.

Panax Ginseng contains amino acids, vitamins, organic acids, carbohydrates and various minerals. It is a medicinal herb that has excellent effect in preventing and treating various diseases such as tonic action, thirst relief, strengthening of immune function, blood pressure, diabetes, .

Ginseng contains 50-70% of carbohydrates, 10-15% of crude protein, 3-8% of crude saponin and 3-7% of ash, and the major physiologically active ingredients are saponin, phenolic component, polyacetylene component, Alkaloid components, polysaccharides and the like are known. Among them, ginseng saponin, which is called "ginsenoside", is named among the saponins of ginseng among the various saponins of plants. Ginseng saponin is a glycoside composed of glycone and aglycone. Up to now, about 30 species have been isolated and their structure has been identified.

Red ginseng is produced by steam-drying the roots of ginseng. During the process of extracting ginseng or extracting ginseng, co-products (such as ginseng extract or red ginseng) are produced. The ginseng liquid is a liquid which is formed on the surface of ginseng when it is cooked. Ginseng is relatively small in amount but contains ginsenosides such as glycosides, polyacetylenic compounds, nitrogenous compounds, flavonoids, vitamins (B group), trace elements, enzymes, antioxidants, organic acids and amino acids . Like the ginseng, the ginseng has sedative and excitatory effects on the central nervous system. It acts on the circulatory system to prevent hypertension and arteriosclerosis. It also reduces hematopoiesis and blood glucose levels, protects the liver, And is known to have a protective effect against radiation.

Ginseng saponin, polyacetylene, antioxidant activity phenolic compounds, gomisin-N, -A, which protects against liver, and acidic peptides that have insulin-like action . In this way, the effective ingredient of the ginseng extract is contained in the extract, and various methods are considered for utilizing the extract.

Several factors are involved in human skin color, and the activity of melanocyte, which makes melanin pigment in particular, is the most important factor. Melanocytes in the human body, such as tyrosinase, act on the melanin and melanin is produced. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin. Thus, melanin produced by stimulation inside and outside of the skin is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stimulus disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results.

However, from the past, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or tyrosinase inhibiting activity have been mixed with cosmetics or medicines in order to inhibit the synthesis or deposition of melanin , Inadequate whitening effect of these ingredients, safety to the skin, formulation and stability in combination with cosmetics, and the like. Therefore, it is necessary to discover natural raw materials that can control the production of melanin effectively, while having few side effects.

Patent Literature 1 discloses a technique of adding a ginseng solution to a pet-supplemented feed. As disclosed in Patent Document 1, there has been no report on the introduction of additional processes for increasing the use of active ingredients contained in the ginseng solution, in particular for whitening purposes, when the ginseng solution is utilized alone. It is true.

The present inventors have studied to utilize the ginseng solution which is a byproduct of red ginseng production. The fraction prepared by fractionating the ginseng solution with basic component after pretreatment of basic component with ginseng solution was compared with the ginseng solution without basic component, And thus the whitening effect was excellent.

Korean Patent No. 1648254

In the present invention, it is an object of the present invention to provide a fraction having a skin whitening effect while reducing raw material cost by using a rosemary solution as a raw material.

In order to solve the above problems, the present invention provides a method for producing ginseng, (V / v) to 8 (v / v)% NaOH aqueous solution to prepare a mixture; Adding butanol to the mixture and fractionating the mixture; And recovering only the butanol layer after fractionation.

The present invention also provides a cosmetic composition for skin whitening comprising the fragrant ginseng fraction produced by the method for producing a fraction of dried ginseng.

The method of manufacturing the ginseng liquid fraction of the present invention increases the skin whitening effect by treating the aqueous solution of NaOH with the aqueous solution of ginseng while reducing the cost by utilizing the ginseng solution which is a byproduct which is formed on the surface when the ginseng is raw as a raw material, It can be useful in the cosmetics field that you need.

FIG. 1 is a graph comparing skin whitening effects of the fresh ginseng liquid fraction (experimental group) produced by the production method of the present invention and the fraction prepared by another production method. (V / v) to 8 (v / v) in the preparation method of the present invention, and the control group 1 is a concentrated solution of the rosemary solution, the control group 2 is a fraction prepared by excluding the NaOH treatment step in the preparation method of the present invention, Means a fraction prepared using 10% NaOH aqueous solution instead of aqueous NaOH solution.
FIG. 2 is a graph showing the results obtained by using the other acetic acid component instead of the aqueous solution of 3 (v / v) to 8 (v / v)% NaOH in the rosemaking liquid fraction (experimental group) (Control group 4) prepared by the method of the present invention.
Fig. 3 shows the whitening effect of a concentrated solution of a red ginseng water extract or a concentrated butanol fraction of a red ginseng water extract. In the control group 1, the concentrate of the red ginseng solution, the red ginseng concentrate of the red ginseng extract, the control group 2, the butanol fraction of the rosemaking concentrate prepared using the butanol fractionation process except the NaOH treatment step, 'Red ginseng concentrate butanol fraction' refers to the butanol fraction of the concentrate of red ginseng water extract.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for producing ginseng, (V / v) to 8 (v / v)% NaOH aqueous solution to prepare a mixture; Adding butanol to the mixture and fractionating the mixture; And recovering only the butanol layer after fractionation.

The present invention also provides a composition for skin whitening comprising the fragrant ginseng fraction prepared by the above-mentioned method for producing a fraction of dried ginseng.

The ginseng solution is a by-product formed on the surface of the ginseng when it is cooked, and includes an active ingredient present on the surface of ginseng or ginsenoside.

The ginseng may be commonly used ginseng and preferably ginseng such as Panax ginseng CA Meyer, Panax quinquefolium, Panax notoginseng, Panax japonicum, Panax trifolium or Himalaya Ginseng such as Panax pseudoginseng or processed ginseng such as red ginseng.

The aqueous solution of 3 (v / v)% to 8 (v / v)% NaOH may be contained in an amount of 5 to 30 parts by weight, preferably 5 to 20 parts by weight, And more preferably 5 parts by weight to 15 parts by weight, but is not limited thereto. When the NaOH aqueous solution is contained below the lower limit value (control group 2) or when it exceeds the upper limit value (control group 3), the whitening effect is reduced (see FIG.

The concentration of the NaOH solution may preferably be 3.5 (v / v)% to 7.5 (v / v)% and more preferably the concentration of the NaOH solution may be 4 (v / )%, And even more preferably 4 (v / v)% to 6 (v / v)%, but is not limited thereto. If the concentration of NaOH is out of the above range, the skin whitening effect is decreased.

In one embodiment of the present invention, when the whitening effect of the fresh ginseng liquid fraction according to the production method of the present invention is confirmed, it is preferable that the ginseng solution itself or 3 (v / v) to 8 (v / (v / v)% NaOH aqueous solution instead of 3% (v / v)% to 8% (v / v)% NaOH aqueous solution in the process of the present invention, The skin whitening effect was superior to that of NaOH aqueous solution treatment and whitening effect was maintained even at low concentration.

The organic solvent used in the present invention may be selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, chloroform, and a mixed solvent of these organic solvents and water, preferably butanol.

In one embodiment of the present invention, the fraction of the fresh ginseng solution obtained by fractionation with butanol after 3 (v / v)% to 8 (v / v)% NaOH aqueous solution treatment reduced the synthesis of melanin (see FIG.

The butanol may be included in an amount of 5 parts by weight to 30 parts by weight, preferably 5 parts by weight to 20 parts by weight, more preferably 5 parts by weight to 10 parts by weight, based on 10 parts by weight of the mixture, But is not limited thereto. When the content of butanol is below the lower limit, the active ingredients converted by NaOH treatment are not sufficiently fractionated. When the content of butanol exceeds the upper limit, the time required for the subsequent concentration increases excessively, and the reprocessing is repeated once or several times in order to remove the residual solvent.

The fresh ginseng fraction of the present invention inhibits melanin production of skin induced by UV or inflammation and improves pigmentation. For example, symptoms such as spots, freckles, and spots are improved.

The rosemary extract fraction of the present invention may be contained in the cosmetic composition in an amount of 0.01 to 30% by weight, preferably 0.01 to 20% by weight, more preferably 0.01 to 10% by weight, 0.01 to 5% by weight. If the content is less than 0.01% by weight, the effect of the fractions may be insignificant. If the content of the fractions exceeds 30% by weight, the effect of the fractions may be relatively low.

The cosmetic composition may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetics field. Liquid or solid cosmetics may include, for example, but not limited to, lotions, creams, lotions, bath salts, and the like.

The base, adjuvant and additives commonly used in the cosmetics field are not particularly limited, and examples thereof include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin and the like.

The cosmetic composition may contain components commonly used in cosmetic compositions as well as the fragrance of the fresh ginkgo extract of the present invention and may contain conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes, . ≪ / RTI >

The cosmetic composition may be in the form of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing oil , A powder foundation, an emulsion foundation, a wax foundation, and a spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the cosmetic composition is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition is an interface-active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The cosmetic composition may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. The cosmetic composition according to the present invention may be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

When the cosmetic composition is a soap, a surfactant-containing cleansing agent or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or washed off with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in detail with reference to Production Examples and Examples.

However, the following Preparation Examples and Examples are for illustrating the present invention, and the content of the present invention is not limited by the following Production Examples and Examples.

≪ Preparation Example 1 > Preparation of a fraction by pretreating a fresh ginseng solution with a basic solution

<1-1> Manufactured by treating 5% aqueous NaOH solution

After washing the ginseng and drying it, the ginseng solution produced in the ginseng process was supplied by Korean ginseng corporation Samchang. The vinegar solution was concentrated at 52 캜 and concentrated until the sugar content of the concentrate reached 58 brix. The solid content of the obtained ginseng concentrate was about 52%. 10 parts by weight of a 5% NaOH aqueous solution was added to 1 part by weight of the concentrate, and the mixture was dissolved at room temperature for 1 hour. An equal volume of saturated butanol was added to the vinegar solution dissolved in the NaOH aqueous solution and fractionated. Only the butanol layer was recovered after the fractionation. The recovered butanol layer was concentrated under reduced pressure at 50 ° C using a vacuum concentrator to completely remove the butanol solvent, then redissolved in purified water, completely frozen at -70 ° C, and lyophilized for 24 hours.

<1-2> Manufactured by treating with 10% aqueous NaOH solution

The fractions of the ginseng solution were prepared in the same manner as in Preparation Example 1, except that the concentration of the aqueous 5% NaOH solution was changed.

<1-3> Manufacture by treating other alkali components instead of NaOH aqueous solution

Fractions of the rosemary extract were prepared in the same manner as in Preparation Example 1, except that 5% Alkarol (Alkarbis) aqueous solution was used instead of 5% NaOH.

&Lt; Example 1 > Evaluation of skin whitening efficacy

The skin whitening efficacy of the fraction obtained in Production Example 1-1 was confirmed. B16 / F1 melanoma cells (ATCC, USA) were grown to 10 passages, and then 5 × 10 ⁴ cells were dispensed into 6-well plates. DMEM medium containing 10% bovine serum was cultured in a 5% CO ₂ incubator Respectively. After 24 hours, the control and experimental groups were treated with 0.0625 mg / ml and 0.03125 mg / ml, respectively. As a positive control, p-coumaric acid was treated at a concentration of 50 ppm. After 72 hours, the melanoma cells were physically detached, melanin was dissolved at 70 ° C in 1N NaOH containing DMSO, and the absorbance was measured at 405 nm.

<1-1> Evaluation of skin whitening efficacy by process

Samples (Control 1) in which the rosemary solution of Production Example 1-1 was concentrated and the fraction of Production Example 1-1 were used as experimental groups and the same method as in Production Example 1-1 except that the 5% NaOH aqueous solution was not treated (Control group 2), the fractions of preparation example 1-2 (control group 3) and 50 ppm (positive control group) of p-coumaric acid were compared.

As a result, as shown in Fig. 1, the fraction of Production Example 1-1 showed the best skin-whitening effect in all the concentration ranges as compared with the control groups 1 to 3. In particular, it showed a whitening effect even at a low concentration of 0.03125 (mg / ml). In addition, the whitening effect was superior to that of the positive control group, p-cumaric acid.

From the above results, it was confirmed that the whitening effect of the fraction obtained by pretreating the aqueous solution of NaOH prepared to have a concentration in the range of 3 (v / v)% to 8 (v / v) (Control group 1), when the concentration of NaOH aqueous solution was 10% or more and the butanol fraction was applied (control group 2) in the case of performing the butanol fraction without the NaOH treatment (control group 2) in the case where the NaOH treatment and the butanol fraction were not performed 3 (v / v)% to 8 (v / v)% aqueous NaOH solution treatment to increase the whitening efficacy when using the ginseng solution because the whitening effect is maintained even at low concentration. It was found necessary.

<1-2> Comparison of efficacy when NaOH is replaced with another alkali component

The skin-whitening efficacy of the fraction of Experimental Example 1-1 (experimental group) and the fraction of Preparation Example 1-3 (control 4) using a different alkali component instead of NaOH were compared.

As shown in FIG. 2, although the pH values of the 5% NaOH aqueous solution and the 5% alkaline aqueous solution were similar to each other at 13.0 and 12.5, the whitening efficacies were different and the fractions of Production Example 1-1 using 5% The whitening effect was lower at all concentrations when the aqueous 5% alkalcohol solution was treated, and 0.01525 (mg / ml) and 0.015625 (mg / ml), respectively, , But melanin synthesis was increased.

From these results, it was found that NaOH is more suitable than other alkali components in order to increase the whitening effect of the ginseng solution.

&Lt; Example 2 > Analysis of ginsenoside content of each fraction

The contents of the control groups 1 to 4 and the ginsenosides of the experimental group are shown in Table 1 below.

Name of sample Rb1 Rb2 Rc Rd Rg3s Rg3r Re Rf Rg1 Rg2s Rh1 Control 1 19.33 7.55 9.66 2.36 1.13 2.12 7.19 1.31 3.37 1.30 0.33 Control group 2 122.92 54.30 69.19 19.31 17.47 9.78 38.75 10.47 19.05 13.04 19.15 Experimental group 13.56 9.86 10.11 24.54 44.90 18.47 81.09 25.60 48.76 35.52 16.65 Control group 3 3.65 2.00 1.87 7.69 37.28 11.97 59.48 22.83 45.01 35.75 17.22 Control group 4 195.70 77.37 104.26 34.13 27.19 15.24 52.77 14.43 26.90 17.76 7.70

As can be seen from Table 1, Rg1, Rg3, Rf, Re and the like of the fractions of Production Example 1-1 of the present invention (experimental group) were lower than those of fractions prepared by other methods, It was confirmed that a large change occurs in the content. As a result, it was confirmed that the components that help the whitening effect were strengthened by the process of the present invention, and that the test group showed excellent whitening effect.

&Lt; Comparative Example 1 >

It was confirmed that other extracts could be applied instead of the rosemary extract as a raw material to the fraction preparation method of the present invention because the rosemary extract fraction prepared in Preparation Example 1-1 was excellent in whitening effect. As the other raw materials, a concentrated solution of red ginseng water extract and a concentrated butanol fraction of red ginseng water extract were used, and the whitening effect of a concentrated solution of a red ginseng water extract or a concentrated solution of a red ginseng water extract before 5% NaOH treatment was confirmed.

As shown in Fig. 3, the butanol fraction (red ginseng concentrate fraction in Fig. 3) of the concentrate of the red ginseng water extract (the red ginseng concentrate of Fig. 3) or the red ginseng water extract was not significantly significantly whitening effect. The fractionation experiment through

From the above results, it was found that the whitening effect of the concentrated red ginseng water extract or the butanol fraction of the red ginseng water extract is not superior to that of the control group 1 (rosemary concentrate) not treated with 5% NaOH. Therefore, It was found that it is appropriate to use the vitamins as a raw material.

PREPARATION EXAMPLE 2 Preparation of Cosmetic Composition

<2-1> Preparation of essence

The essence was prepared according to the content (parts by weight) shown in the following Table 2 using the fresh ginseng liquid fraction of Production Example 1-1.

Furtherance Content (parts by weight) Triethanolamine 0.25 Carboxyvinyl polymer 0.22 glycerin 4 Butylene glycol 2 The rosemary extract fraction of Preparation Example 1-1 1.5 Wax 0.5 Cetostearyl alcohol One Glyceryl monostearate One Squalene 4 Purified water Suitable amount

<2-2> Production of softening longevity

The softening longevity of the rosemary extract fraction of Preparation Example 1-1 containing the active ingredient as the active ingredient was prepared as shown in Table 3 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 PEG-40
Hydrogenated castor oil 1.00 ethanol 30.00 The rosemary extract fraction of Preparation Example 1-1 0.5 coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<2-3> Preparation of nutritional cream

The nutritional cream containing the dried ginseng liquid fraction of Production Example 1-1 as an active ingredient was prepared in accordance with the composition shown in Table 4 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalene 3.8 The rosemary extract fraction of Preparation Example 1-1 1.5 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Flavoring agent Suitable amount Purified water Balance

<2-4> Production of Lotion

Lotions containing the fresh ginseng liquid fraction of Production Example 1-1 as an active ingredient were prepared according to the composition shown in Table 5 below.

Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Pro-type glycerin monostearate 1.8 PEG-100 stearate 2.6 Sorbic acid sesquioleate 0.6 Squalene 4.8 Macadia oil 2 Jojoba oil 2 Tocopheryl acetate 0.4 Methylpolysiloxane 0.2 Tocopheryl acetate 0.4 1,3-butylene glycol 4 Xanthan gum 0.1 glycerin 4 d-Pandenol 0.15 The rosemary extract fraction of Preparation Example 1-1 1.0 Allantoin 0.1 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 Purified water Suitable amount

Claims (7)

Preparing a ginseng solution of ginseng;
(V / v) to 8 (v / v)% NaOH aqueous solution to prepare a mixture;
Adding butanol to the mixture and fractionating the mixture; And
And recovering only the butanol layer after fractionation.
The method according to claim 1,
Wherein the concentration of the NaOH aqueous solution is 3.5 (v / v)% to 7.5 (v / v)%.
The method of claim 2,
Wherein the concentration of NaOH aqueous solution is 4 (v / v)% to 7 (v / v)%.
The method according to claim 1,
Wherein the butanol is contained in an amount of 5 to 30 parts by weight based on 10 parts by weight of the mixture.
The method according to claim 1,
Wherein the butanol is contained in an amount of 5 to 20 parts by weight based on 10 parts by weight of the mixture.
The method according to claim 1,
Wherein the butanol is contained in an amount of 5 to 10 parts by weight based on 10 parts by weight of the mixture.
A cosmetic composition for skin whitening comprising the fraction of red ginseng liquor produced by the method of any one of claims 1 to 6.

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