KR20190025863A - Cosmetic or phamaceutical composition comprising herb extract and method for preparing the same - Google Patents

Abstract

The present invention relates to a cosmetic or pharmaceutical composition comprising, as an active ingredient, an extract of a mixture comprising Prunus persica leaves, Morus alba leaves, Styphnolobium japonicum roots, Prunus mume fruits, and Salix pierotii bark. Accordingly, the cosmetic or pharmaceutical composition containing five herbal medicine extracts is excellent in skin soothing effects, without irritation to skin, such as reduction of trans-epidermal water loss and alleviation of skin redness, and exhibits a skin temperature lowering effect, a skin moisturizing effect, and an anti-inflammatory effect, thereby being suitable for use in sensitive skin.

Description

TECHNICAL FIELD The present invention relates to cosmetic or pharmaceutical compositions containing herbal extracts and methods for preparing the same,

The present invention relates to a cosmetic or pharmaceutical composition containing a herbal extract and a method for producing the same, and more particularly, to a cosmetic or a pharmaceutical composition containing a herbal extract mixed with a plurality of kinds and introducing a pretreatment in the production of an extract to provide skin moisturizing, And a pharmaceutical composition and a method for producing the same.

The skin, which is the largest organ of the human body, occupies about 16% of the entire human body volume. It is directly contacted with the external environment, and protects the human body from many harmful factors that intrude into the human body such as temperature, humidity, dust, It plays an important protective role. However, infants or sensitive adults are easily damaged by various pollutants, strong ultraviolet rays, stress, etc., and it takes time to recover. In recent years, there have been an increasing number of people who complain of skin irritation such as erythema, edema, acne, and itch, thereby reducing skin irritation and inflammation caused by various irritants and reducing anti-inflammatory and sedative It is urgently required to develop a substance having an excellent effect.

One of the biggest skin troubles of sensitive modern man is atopic symptoms. Symptoms of these atopic dermatitis are severe itching. In acute phase, the skin is itchy, reddish, swollen, sprouting up like small rice, or catching small blisters, and the scabs sit. The chronic period is itchy, and the skin becomes hard, thick, streaked, scaly like rice flour, satiated, blackened or whitened. Atopic dermatitis usually begins with an infantile eczema, often called a fever. Among steroids currently in use, steroids are highly effective in anti-inflammatory and immunosuppressive actions, but there are concerns about side effects. If applied for a long time, the skin of the right area can be shrunk, the skin pigmentation is reduced, the infection of the germ can occur, the acne may grow and the skin may become thinner. In severe cases, systemic symptoms due to hormones may occur. Since the age of onset of atopic dermatitis is common in infancy before the age of 5, it is recommended to use a small amount of steroids, rather than a lot of one at a time, when the steroid is used only for a short period of time.

 Atopic dermatitis is a more frightening disease with secondary bacterial infections that are more itchy than scratching itself. The skin of atopic patients comes with bacterial infections as a result of long-term rubbing and drying. More than 90% of patients with atopic disease are infected with Staphylococcus aureus, which can be caused by scratching the patient's itch. However, recent reports indicate that the bacteria 's exotoxin stimulates the body' s immune system, causing allergic chemicals It is said that it makes the atopy worse. Therefore, the use of appropriate antibiotics is essential for the treatment of atopy.

 However, since there is a fear of adverse side effects of steroid drugs, studies on natural drug preparations have been actively conducted recently. Preparations made from natural products have few side effects and their action is generally mild, persistent, biodegradable and environmentally friendly.

 Examples of natural product preparations for sensitive skin include cosmetic compositions for improving healing of atopic skin including white wool, barley, Echinacea, and fermented soybean extract, a cosmetic composition for improving healing of atopic skin, The present invention relates to a method for the production of a herbal composition, which comprises culturing a crude herb extract comprising a herbal extract, Angelica keiskei koidz A composition for external application for skin containing a fermented herbal extract having an excellent effect on skin improvement such as moisturizing, skin irritation alleviation, atopic improvement, and anti-inflammatory effect.

However, the development of a natural formulation which does not have side effects while providing moisturizing effect, anti-inflammation and itching relief, which are essential elements of a therapeutic agent for sensitive skin, is insufficient and research is needed.

Korean Patent No. 0431076 Korea Patent No. 1081914

It is an object of the present invention to provide a herbal medicine extract which is excellent in skin soothing effects such as reduction of transdermal water loss and skin redness without irritation to the skin and exhibits skin temperature lowering, To provide a cosmetic or pharmaceutical composition containing the same.

Another object of the present invention is to prepare a cosmetic composition or a pharmaceutical composition containing a mixed extract having an optimal combination of herbal medicine in the production of a herbal medicine extract and to maximize the improvement effect of various skin problems by introducing a dyeing method in a conventional herbicidal process have.

According to an aspect of the present invention,

Peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And an extract of a mixture containing willow bark; as an active ingredient.

The mixture may be a chlorinated mixture.

The chlorine treatment may be a process in which the material is immersed in salt water and then subjected to scouring.

The quenching may be carried out at 80 to 120 ° C.

The calcination process may be performed for 20 minutes to 40 minutes.

The mixed extract may contain 10 to 40,000 parts by weight of mulberry leaves per 100 parts by weight of peach tree leaves; 10 to 20,000 parts by weight of a tree root; From 0.5 to 5000 parts by weight of plum blossom; And 10 to 30,000 parts by weight of willow bark.

The extract of the mixture may contain one or more extracts selected from the group consisting of purified water, glycerin, butyleneglycol, hexanediol, methylpropanediol, propanediol, propylene glycol, ethanol, methanol, ethyl acetate, dichloromethane, ethyl ether, And may be one extracted with a solvent.

The cosmetic composition may have at least one of application for skin moisture improvement, skin moisturizing enhancement, skin soothing, anti-inflammation, atopic amelioration, itching improvement, and skin temperature reduction.

The extract of the mixture may be contained in an amount of 0.0001 to 90% by weight based on the total weight of the cosmetic composition.

According to another aspect of the present invention,

Extracting a component of a material comprising at least one selected from the group consisting of peach tree leaves, mulberry leaves, painting tree roots, plum blossom, and willow bark.

(A) preparing a washed material by washing a material containing at least one selected from a peach tree leaf, a mulberry leaf, a painting tree root, a plum tree fruit, and a willow bark; (b) immersing the washed material in salt water and then subjecting the washed material to a salt treatment to prepare a chlorinated material; (c) pulverizing the chlorinated material and extracting it with an extraction solvent to obtain a filtrate; And (d) concentrating and drying the filtrate to prepare a dried extract powder.

Wherein the material of step (a) comprises, relative to 100 parts by weight of peach tree leaves, 10 to 40,000 parts by weight of mulberry leaves; 10 to 20,000 parts by weight of a tree root; From 0.5 to 5000 parts by weight of plum blossom; And 10 to 30,000 parts by weight of willow bark.

The brine of step (b) may be in a concentration of 2% to 4%.

Step (c) can be carried out by stirring at 80 to 120 캜 for 20 to 40 minutes.

The extraction solvent of step (d) is selected from the group consisting of purified water, glycerin, butylene glycol, hexanediol, methylpropanediol, propanediol, propylene glycol, ethanol, methanol, ethyl acetate, dichloromethane, ethyl ether, It may be more than one kind.

After step (e), the extraction powder may be dissolved in an extraction solvent to prepare an extract.

According to another aspect of the present invention,

Peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And an extract of a mixture containing willow bark; as an active ingredient, an anti-inflammatory pharmaceutical composition is provided.

Also, peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And an extract of a mixture comprising willow bark; as an active ingredient, a pharmaceutical composition for improving or treating atopy.

Also, peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And an extract of a mixture containing willow bark; as an active ingredient, a pharmaceutical composition for improving or treating itching is provided.

According to another aspect of the present invention,

Extracting a component of a material comprising at least one selected from the group consisting of peach tree leaves, mulberry leaves, painting tree roots, plum fruit, and willow bark.

The cosmetic or pharmaceutical composition of the present invention includes five herbal medicine extracts, and is excellent in skin sedative effects such as reduction of transdermal water loss, alleviation of skin redness without irritation to the skin, lowering of skin temperature, improvement of skin moisturizing effect and anti- It is very suitable for use on sensitive skin.

In addition, the method of producing a cosmetic composition or a pharmaceutical composition of the present invention can produce a mixed extract having an optimal combination of herbal medicine in the production of herbal extracts, and can maximize the improvement effect of various skin problems by introducing a salt- have.

Fig. 1 shows the cell migration effect analysis result of Test Example 1. Fig.
Fig. 2 shows the results of the water content improving effect analysis of Test Example 2. Fig.
Fig. 3 shows the results of the moisturizing and improving effect analysis of Test Example 3. Fig.
4 shows the results of the analysis of the anti-inflammatory effect of Test Example 4. Fig.
Fig. 5 shows the result of the atopy inhibitory effect analysis of Test Example 5. Fig.
Fig. 6 shows the results of analysis of transdermal water loss in the skin soothing effect of Test Example 6. Fig.
Fig. 7 shows the analysis result of the redness reduction effect in the skin soothing effect of Test Example 6. Fig.
Fig. 8 shows the result of the skin temperature reduction effect test of Test Example 7. Fig.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprises" or "having" are used to specify that a feature, a number, a step, an element, or a combination thereof is to be described, But do not preclude the presence or addition of steps, components, or combinations thereof.

Hereinafter, the cosmetic composition of the present invention will be described.

The cosmetic composition of the present invention comprises peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And willow bark; as an active ingredient.

It is preferable that the mixture is subjected to a chlorine treatment.

The chlorine treatment means that the saltwater is dipped in the salt water and then subjected to a drenching process.

The calcination is preferably performed at 80 to 120 ° C for 20 minutes to 40 minutes.

The mixed extract may contain 10 to 40,000 parts by weight of mulberry leaves per 100 parts by weight of peach tree leaves; 10 to 20,000 parts by weight of a tree root; From 0.5 to 5000 parts by weight of plum blossom; And 10 to 30,000 parts by weight of willow bark.

The peach tree leaves contain nitrile glycosides, 0.07-0.2% tannin, and are rich in aspartic acid, lycopene, and the like.

It is preferable that the mixed extract includes a leaf part of a part of a peach tree, but other parts may be applied depending on the case. Peach tree flowers contain chemperol and its glycosides, tribasic acid and dioxybasic acid. The seeds contain about 30% fetishin and about 1.5% of amigalline. The bark contains naringenin, triacontane, And pentatriacontane, and it contains 40 to 50% of oil (main components are olein, palmitin, stearin). Flowers are used for diuretic therapy and constipation therapy in the treatment of bronchial asthma, and in the private sector, leafy branches are put into a kiln and used for manure and eczema.

The fruit of the plum tree is called plum, and the fruit is round and yellow when yellow. There are lemon, malic, vine, succinic acid, sitosterol, oleic acid, cetyl alcohol and seed amidgalin in the fruit acid, and the raw fruit is used as a raw material of lemon acid. Dry fruit contains 19% of lemon acid and 1.5% of malic acid. In migraine treatment, it is used as a calming concentrate, diarrhea, fever, cough medicine, and phlegm (herbal ingredients and use, January, 1999, 358). The plum contains hysperidin and catechin acid, which can be effective for skin inflammation.

 Morus alba, Linne, Morus alba for Peldulls DIPPEL, Morus bombycis, Koidzumi, and Mulberry trees are the most common species of mulberry trees. Morus bombycis var. Caudatifolia, Morus bombycis var. Maritima KOIDZ, Morus tiliaefolia, Makino, which are deciduous broad - leaved arboreous trees distributed horizontally throughout most of Korea, It grows in the foot of the mountain. The upper limbs are usually bitter and have the efficacy to remove inflammation, diuretic, tongue, sweating and wind, and have been widely used as herbal medicines for a long time. Especially, mulberry leaves are rich in polyphenols and have high antioxidative effect. They are effective in strengthening the skin barrier by abundant lipid, which is one of the important components of the skin barrier component.

 Painted wood (Sophora Japonica L) belongs to the soybean family, and is distributed in Korea, Japan, and China, with a height of 25m. Quercetin is abundant in the roots of painting trees and is effective in moisturizing the skin (Colloids and Surfaces B: Biointerfaces, Volume 123, 1 November 2014, Pages 778-786).

 The willow (Salix koreensis Andersson) is also known as willow or weeping will, with 3 ~ 10% of tannin in the skin, flavon, ascorbic acid, salicylic acid. Salicin contains up to 5% and is degraded to saline and glucose by enzymes. Willow has a component (salicyl) that is similar to the main active ingredient of aspirin, so it acts to relieve fever and relieve inflammation and pain. Willow bark is rich in salicylic acid, so it can be effective for skin inflammation.

The extract of the mixture is extracted with an extraction solvent such as purified water, glycerin, butyleneglycol, hexanediol, methylpropanediol, propanediol, propylene glycol, ethanol, methanol, ethyl acetate, dichloromethane, ethyl ether, However, the scope of the present invention is not limited thereto, and various extraction solvents that can be used for extraction of plant material can be used.

The cosmetic composition can be used for skin moisture improvement, skin moisturizing enhancement, skin soothing, anti-inflammation, atopic amelioration, itching improvement, skin temperature reduction and the like.

The cosmetic composition may be an extract of the mixture, a powder obtained by freeze-drying the extract of the mixture in vacuo, concentration, or a combination of the powder and an extraction solvent.

The extract of the mixture may preferably be contained in an amount of 0.0001 to 90% by weight based on the total weight of the cosmetic composition. This is because when the content of the extract is less than 0.0001% by weight, the skin soothing effect is hardly exhibited, and when it is more than 90% by weight, the stability on the formulation may be lowered.

Hereinafter, the method for producing the cosmetic composition of the present invention will be described.

The method for producing a cosmetic composition of the present invention comprises extracting a component of a material comprising at least one selected from the group consisting of peach tree leaves, mulberry leaves, tree roots, plum blossom, and willow bark.

Specifically, first, the washed material is prepared by washing a material including at least one selected from peach tree leaves, mulberry leaves, roots of painting trees, fruit of plum, and willow bark (step a).

Wherein the material is selected from the group consisting of 10 to 40,000 parts by weight of mulberry leaves, 10 to 20,000 parts by weight of a tree root; From 0.5 to 5000 parts by weight of plum blossom; And 10 to 30,000 parts by weight of willow bark.

Thereafter, the washed material is immersed in brine, and then a crystallization process is performed to prepare a chlorine-treated material (step b).

The salt water is preferably in a concentration of 2% to 4%.

The calcination is preferably carried out at 80 to 120 DEG C for 20 to 40 minutes.

Thereafter, the seed treated material is pulverized and then extracted with an extraction solvent to obtain a filtrate (step c).

The extract of the mixture is extracted with an extraction solvent such as purified water, glycerin, butyleneglycol, hexanediol, methylpropanediol, propanediol, propylene glycol, ethanol, methanol, ethyl acetate, dichloromethane, ethyl ether, And more preferably, purified water, methylpropanediol, or hexanediol can be used. The scope of the present invention is not limited thereto, and various extraction solvents that can be used for extraction of plant materials can be used.

Thereafter, the filtrate is concentrated and dried to prepare dried extract powder (step d).

The concentration is preferably carried out by reduced-pressure concentration, and the drying can be performed by lyophilization, but the scope of the present invention is not limited thereto.

Thereafter, the dried extract powder may be dissolved in an extraction solvent to prepare an extract.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredients, the ingredients conventionally used in cosmetic compositions, for example, conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, And includes pancakes. The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil , A powder foundation, an emulsion foundation, a wax foundation, etc. However, the present invention is not limited thereto. More specifically, it can be manufactured in the form of cleansing cleansing lotion, cream, massage cream, eye cream, essence, gel, pack, spray, powder, sunscreen and the like.

 When the formulation of the present invention is a paste, a cream or a gel, vegetable oil, wax, starch, cellulose, silicone, bentonite, silica, zinc oxide or the like may be used as a carrier component. , Aluminum hydroxide, calcium silicate and the like may be used. When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, glycerin, ethyl carbonate, ethyl acetate, propylene glycol, butyleneglycol, , 2-hexanediol, glycerol aliphatic ester, and sorbitan fatty acid ester.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol, propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, etc. may be used. Also, when the formulation is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, methyl taurate, sarcosinate, alkylamido betaine, aliphatic alcohol, Rid, vegetable oil, or ethoxylated glycerol fatty acid ester.

The present invention also relates to a process for the production of peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And a willow bark. The present invention also provides a pharmaceutical composition comprising the extract as an active ingredient.

The present invention also relates to a process for the production of peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And an extract of a mixture comprising willow bark; as an active ingredient, a pharmaceutical composition for improving or treating atopy.

The present invention also relates to a process for the production of peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And a willow bark; as an active ingredient, a pharmaceutical composition for improving or treating itching.

The extract of the mixture is the same as that of the cosmetic composition of the present invention described above, so that details thereof will be referred to for the details.

In addition, the present invention provides a method for preparing a pharmaceutical composition comprising extracting a component of a material comprising at least one selected from the group consisting of peach tree leaves, mulberry leaves, nursery tree roots, plum tree fruit, and willow bark .

The method of extracting the above components is the same as that of the above-described method for producing a cosmetic composition, and therefore, the details thereof will be referred to.

The pharmaceutical composition of the present invention may be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, Or a flavoring agent may be used.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

The pharmaceutical form of the pharmaceutical composition may be liquid, syrup, juice, suspension, emulsion, drip agent, or the like. For example, also, if necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.

The pharmaceutical composition of the present invention is preferably administered by transdermal administration.

[Example]

Example  One: A seed  Manufacture of herbal extracts treated

(1) Material cleaning

Peach tree leaf, plum tree fruit, mulberry leaf, painting tree roots, and willow bark, respectively.

(2) Treatment of chlorophyll

Then, the mixture was mixed well with a 3% brine solution prepared using cleanly purified salt, and the mixture was sealed and allowed to stand for 3 hours to allow salt to penetrate. Then, peach tree leaves, plum blossom, mulberry leaves, Painted tree roots and willow bark were shaken at a temperature of about 100 ° C for 30 minutes and then dried in the shade.

(3) Herbal extracts

Then, the mixture was crushed and passed through a 300-mesh sieve to finely crush the crushed material. Then, ethanol was added so that the final concentration became 60% (v / v) ethanol aqueous solution, refluxed twice for 3 hours, cooled, and filtered with whatman # 2 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized.

Specifically, the filtered extract was concentrated using a rotary vacuum concentrator, and 600 ml of the extract was placed in a 1,000 ml round-bottomed flask, and the mixture was concentrated under reduced pressure for 2 hours while rotating at 1000 rpm at a pressure of 700 mmHg .

The concentrate under reduced pressure was subjected to preliminary freezing to lower the temperature to -40 ° C., maintain it at -40 ° C. for 3 hours, freeze trapped to -80 ° C., maintain the vacuum at 20 mTorr, Raise it to 40 ℃ again. Thereafter, the mixture was kept at -20 ° C for 4 hours, at 0 ° C for 1 hour, at 15 ° C for 6 hours, at 30 ° C for 2 hours, and maintained at 20 hours until completely dried to prepare a lyophilized powder.

The lyophilized powder was dissolved in a concentration of 1% (v / v) and 10% (v / v) using 10% methylpropanediol and 2% 1,2- .

Example  2: Heat number  Preparation of herbal extracts

A crude herbal extract was prepared in the same manner as in Example 1, except that the seedling treatment was omitted.

[Test Example]

Test Example  1: cell migration ( Cell Migration ) Effect analysis

1% (v / v) extract (Example 1) of Example 1 and 1% (v / v) extract (DW 1) of Example 2 were added to HDFn cells (human dermal fibroblast neonatal cell) And the cell migration effect was observed after 24 hours.

FIG. 1 shows an optical microscope image of the herbal medicine extract compared with the hydrothermal extract of Example 2 to confirm the cell migration effect of the herbicide-treated herb extract according to Example 1. According to FIG. 1, as a result of cell migration evaluation in HDFn cells, the extract obtained by further performing the dyeing treatment of Example 1 was superior to the hot-water extract of Example 2 in cell migration.

Experimental Example  2: Analysis of water content improvement effect

(V / v) extract (Example 1) of Example 1, 10% (v / v) extract (Example 10) of Example 1 and 1% (v / v) of Example 2 (AQP3) and hyaluronic acid (HAS3) synthase genes involved in skin moisture content were evaluated by adding the extract (DW 1) extract of Example 2 and the 10% (v / v) extract (DW 10) The results are shown in Fig. Here, "ra" means retinol. 2, the herbal medicine extract of the present invention was shown to be effective in improving the skin moisture, and in particular, the herbal medicine extract of Example 1, in which the salt treatment was further performed, further enhanced the expression of aquaporin and hyaluronic acid synthase gene appear.

Experimental Example  3: Analysis of moisturizing improvement effect

(V / v) extract (Example 1) of Example 1, 10% (v / v) extract (Example 10) of Example 1 and 1% (v / v) of Example 2 ) Extracts (DW 1) and the 10% (v / v) extract (DW 10) of Example 2 were added to evaluate the expression of cladin gene involved in the keratin differentiation in papilloma and cell- The results are shown in Fig. According to FIG. 3, it is possible to increase the rate of expression of clodin gene involved in pillared green and cell-cell binding force involved in differentiation of keratinocytes in dermal keratinocytes, thereby strengthening skin barrier and consequently improving skin moisturizing effect.

Experimental Example  4: Analysis of anti-inflammatory effect

The human skin keratinocytes were treated with Poly I: C, an inflammation inducing substance, and then treated with 1% (v / v) extract (Example 1) of Example 1 and 10% (v / v) 8 gene expression rate by adding 1% (v / v) extract (DW 1) of Example 2, 10% (v / v) extract (DW 10) of Example 2 and dexamethasone (antihistamine) The results are shown in Fig. According to FIG. 4, the extract of Example 1 and the 10% (v / v) extract of Example 2 were superior to the conventional antihistamine dexamethasone.

Experimental Example  5: Atopy inhibitory effect analysis

The human skin keratinocytes were treated with Poly I: C and IL-4, which are inducers of TSLP (skin-inducing chemicals), and then treated with 1% (v / v) (V / v) extract (DW 10) of Example 1, 1% (v / v) extract (DW 1) of Example 2 and 10% , Dexamethasone (antihistamine agent) was added to measure the expression rate of TSLP gene, and the results are shown in FIG. 5, the TSLP gene expression rate of the herbal medicine extract of the present invention was lower than that of the test group (+) in which no drug treatment was performed, and it was confirmed that the TSLP gene expression was 10% (v / v) In the extract treatment group, the atopy inhibition effect was the best.

Test Example  6: Analysis of skin soothing effect

Twenty subjects who met the selection criteria were selected and tested to demonstrate skin soothing effects. First, 1% (v / v) extract 15 of Example 1 was applied to the skin where the 1% SLS (sodium laureth sulfate) solution was applied to the skin and the area of increased transdermal water loss (TEWL) and skin- The results are shown in FIG. 6 and FIG. 7, respectively. The results are shown in FIG. 6 and FIG. 7, respectively. The results are shown in FIG. 6 and FIG. 7, respectively, after 24 hours and 48 hours after application of the herbal medicine extract. According to Figs. 6 and 7, the amount of transdermal water loss was significantly reduced to a level (p <0.05) statistically significant compared with the control site after 24 hours and 48 hours, and skin- (P < 0.05) after 24 hours of the patch compared to the control area, indicating that the 1% (v / v) extract of Example 1 was superior in skin soothing effect.

Test Example  7: Skin temperature reduction effect analysis

In order to demonstrate the effect of lowering the skin temperature, a human body application test was conducted. First, 21 subjects who matched the selection criteria were selected, and 1% (v / v) extract of Example 1 was applied to the face of the subject, and skin temperature changes were analyzed using a thermal imaging camera. The results are shown in FIG. . According to FIG. 8, it was judged that the skin-temperature reduction effect of the region where the sample was applied was decreased to a statistically significant level (p < 0.05) immediately after the application of the sample, All showed an average temperature reduction of 2.5 °.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, many modifications and changes may be made by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims. The present invention can be variously modified and changed by those skilled in the art, and it is also within the scope of the present invention.

Claims (20)

Peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And a willow bark; as an active ingredient. The method according to claim 1,
Wherein the mixture is a salt-treated mixture. 3. The method of claim 2,
Characterized in that the salt treatment is carried out by immersing the salt in a salt water and then finishing the salt treatment. The method of claim 3,
Wherein the softening process is performed at 80 to 120 ° C. 5. The method of claim 4,
Wherein the calcination is carried out for 20 minutes to 40 minutes. The method according to claim 1,
The above-
With respect to 100 parts by weight of peach tree leaves,
10 to 40,000 parts by weight of mulberry leaves;
10 to 20,000 parts by weight of a tree root;
From 0.5 to 5000 parts by weight of plum blossom; And
And 10 to 30,000 parts by weight of a willow bark. The method according to claim 1,
The extract of the mixture may contain one or more extracts selected from the group consisting of purified water, glycerin, butyleneglycol, hexanediol, methylpropanediol, propanediol, propylene glycol, ethanol, methanol, ethyl acetate, dichloromethane, ethyl ether, Wherein the cosmetic composition is extracted with a solvent. The method according to claim 1,
The cosmetic composition of the present invention has at least one of the following applications: skin moisturizing, skin moisturizing, skin cleansing, anti-inflammation, atopic amelioration, itching improvement and skin temperature reduction. The method according to claim 1,
Wherein the extract of the mixture is contained in an amount of 0.0001 to 90% by weight based on the total weight of the cosmetic composition. Extracting a component of a material comprising at least one selected from the group consisting of peach tree leaves, mulberry leaves, painting tree roots, plum blossom, and willow bark. 11. The method of claim 10,
The method for producing the cosmetic composition,
(a) preparing a washed material by washing a material comprising at least one selected from the group consisting of peach tree leaves, mulberry leaves, painting tree roots, plum blossom, and willow bark;
(b) immersing the washed material in salt water and then subjecting the washed material to a salt treatment to prepare a chlorinated material;
(c) pulverizing the chlorinated material and extracting it with an extraction solvent to obtain a filtrate; And
(d) concentrating and drying the filtrate to prepare a dried extract powder. 12. The method of claim 11,
The material of step (a)
With respect to 100 parts by weight of peach tree leaves,
10 to 40,000 parts by weight of mulberry leaves;
10 to 20,000 parts by weight of a tree root;
From 0.5 to 5000 parts by weight of plum blossom; And
And 10 to 30,000 parts by weight of a willow bark. 12. The method of claim 11,
Wherein the brine of step (b) is in a concentration of 2% to 4%. 12. The method of claim 11,
Wherein step (c) is carried out by shaking at 80 to 120 DEG C for 20 to 40 minutes. 12. The method of claim 11,
The extraction solvent of step (d) is selected from the group consisting of purified water, glycerin, butylene glycol, hexanediol, methylpropanediol, propanediol, propylene glycol, ethanol, methanol, ethyl acetate, dichloromethane, ethyl ether, Wherein the cosmetic composition is at least one kind of cosmetic composition. 12. The method of claim 11,
And (e) after the step (e), dissolving the extracted powder in an extraction solvent to prepare an extract. Peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And an extract of a mixture containing willow bark; as an active ingredient. Peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And an extract of a mixture containing willow bark; as an active ingredient. Peach tree leaves; Mulberry leaves; Painting tree roots; Plum tree fruit; And a willow bark; as an active ingredient, an extract of the mixture. Extracting a component of a material comprising at least one selected from the group consisting of peach tree leaves, mulberry leaves, painting tree roots, plum blossom, and willow bark.

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