KR102122960B1 - Black Platycodon Grandiflorum root and Black Angelica Sinensis root, Black Zingiber Officinale (Ginger) Root complex with an anti-inflammatory, anti-allergic and atopic skin improvement, a preparation method thereof and a cosmetic composition of the same - Google Patents

Abstract

The present invention relates to a method for preparing black bellflower, black Angelica, and black ginger extracts having anti-inflammatory, anti-allergic and atopy skin improvement effects, specifically extracting, concentrating, and extracting 9 to 9 bellflowers, Angelica and ginger. Providing a powder, free radical scavenging in the cells, inhibiting the activity of lipid peroxidation, lipoxygenase, hyaluronidase, and providing a cosmetic composition containing the black bellflower and black donkey extract powder, thereby inducing skin inflammation , It improves the allergic and atopy skin, and relates to a cosmetic composition containing an extract of black bellflower, black donkey, black ginger, which has anti-inflammatory, anti-allergic and atopy skin-improving effects to prevent skin irritation.
In addition, the present invention is an anti-inflammatory, anti-allergy and atopy skin improvement effect, black bellflower, black donkey, characterized by extracting 9 or 9 medicinal herbs selected from at least one of bellflower, Angelica and ginger with ultrasonic or hot water. Provides a mixture of black ginger.
In addition, the present invention is a mixture of bellflower, Angelica, and ginger (9, 9) (S10), ultrasonic or hot water extraction to obtain a black bellflower, black Angelica, and black ginger mixed extract (S20), and the black bellflower and It consists of the step (S30) of obtaining a mixture of black Angelica and black ginger, and freeze-drying the extract of Black Bellflower, Black Angelica and Black Ginger (S40). It provides a method of producing black bellflower, black angelica, and black ginger extracts having anti-inflammatory, anti-allergic, and atopic skin-improving effects.
In addition, the present invention provides a cosmetic composition containing a mixed extract of black bellflower, black angelica, and black ginger with anti-inflammatory, anti-allergic, and atopic skin-improving effects, including a fermented seaweed mixture.

Description

Anti-inflammatory, anti-allergic and atopy skin improvement effect of black bellflower, black Angelica, black ginger mixture, preparation method and cosmetic composition thereof Black Platycodon Grandiflorum root and Black Angelica Sinensis root, Black Zingiber Officinale (Ginger) Root complex with an anti-inflammatory, anti-allergic and atopic skin improvement, a preparation method thereof and a cosmetic composition of the same}

The present invention relates to a method for preparing a mixed extract of black bellflower, black Angelica and black ginger having anti-inflammatory, anti-allergic and atopy skin improvement effects, and more specifically, steaming bellflower, Angelica and ginger with steam 9 times and drying 9 times (9 diarrhea 9 packs) extracts, concentrates, and freeze-dried black bellflower, black donkey, and black ginger to provide a mixed extract, thereby eliminating free radicals in cells, lipid peroxidation, and lipoxygenase ), hyaluronidase (hyaluronidase) to inhibit the activity of anti-inflammatory, anti-allergic and atopic skin extracts, and relates to a method of manufacturing the extract.

In addition, the present invention provides anti-inflammatory, anti-allergic to improve skin irritation, allergies and atopy skin, and has no side effects on skin irritation by providing a cosmetic composition containing an extract extracted from 9, 9 and 9 bellflower, Angelica and ginger. And it relates to a cosmetic composition containing a mixed extract having an atopic skin improving effect.

Skin is a body part that is directly exposed to the external environment. When exposed to excessive ultraviolet rays or pollutants, skin irritation and inflammatory reactions such as erythema, edema, and itching are caused. It is known that such skin troubles are not only an aesthetic problem, but also substances that are generated in the inflammatory reaction process cause pigmentation incidentally and promote the collapse of skin elastic fibers, thereby increasing skin wrinkles. It is also known to be associated with free radicals for skin damage.

The theory of free radicals was proposed by D. Harman in the mid-1950s, and has recently attracted attention from academic societies and related industries. Free radicals cause various problems such as wrinkle formation, skin cancer, cell death, rheumatoid arthritis, atopic dermatitis, and acne, because free radicals destroy cells, cut the connective tissue of the dermal layer of the skin, or cause cross-linking.

The causes of free radicals are known as the phagocytosis of white blood cells, the electron transport system during the production of ATP in mitochondria, the action of myeloper oxide (MPO), ultraviolet light, tobacco, normal metabolic processes, stress, pollutants, and bacteria. .

The phagocytosis refers to an action in which a cell body accepts foreign substances or bacteria into its body and decomposes and detoxifies it.

Of course, the human body has antioxidants (radical scavengers), superoxide dismutase (SOD), catalase, vitamin E, vitamin C, and ubiquinol, but the antioxidant system by age, pollution, UV, and stress It becomes neutralized and the free radical gradually increases.

Increased free radicals destroy collagen (Collagen), elastin (Hlasturonic acid) and hyaluronic acid (Hyaluronic acid), which are the connective tissues of the dermis. Destructive phenomena occur, which causes diseases of dermatitis, acne, and skin cancer.

The free radicals are involved in spontaneous oxidation reactions (dopaquinone → melanin) during the melanin formation process, and may cause spots, freckles, and wrinkles. (Journal of the Korean Society of Cosmetics, Vol. 23, No. 1, 75~132)

Inflammation is a physiological response that protects the body from harmful environmental conditions, such as invasion and mechanical damage from foreign objects such as bacteria. This inflammatory phenomenon leads to a large increase in various types of polymorphonuclear leukocytes (PMNs) and immune substances, and these cells can be treated and defended by secreting inflammatory cell products of various types of proteases and cytokines. Enable. Enzymes such as elastase, hyalurinidase and lipoxygenase are well known proteins and lipolytic enzymes produced during inflammation.

On the other hand, their action sometimes causes harmful damage to adjacent tissue cells and non-cellular components, so under appropriate conditions, inflammation returns to normal function after the initial state, but irritants that stimulate inflammation do not disappear or continue. If it is made as a result, chronic inflammation occurs as a result, which causes even more serious tissue damage.

Cells and connective tissue are damaged by proteolytic enzymes caused by excessive inflammation, and damage to connective tissue decreases the elasticity of the skin, causing wrinkles as well as adversely affecting cell regeneration and proliferation. This leads to rapid skin aging. In addition, inflammation in the skin is characterized by local erythema and swelling. When the damage is greater, normal tissues are restored in the dissipation stage by scar tissue, which is the result of proliferation of fibroblasts and accumulation of connective tissue proteins. . However, if tissue is more severely damaged and the inflammatory response persists, restoring normal tissue structure and function becomes much more difficult.

The production of mediators that promote the migration of white blood cells along with inflammation is also achieved by a process with arachidonic acid. This pathway consists of a process by cyclooxygenase and a process by lipoxygenase, and leukotriene and prostagladine produced by these enzymes act as inflammatory factors. Therefore, inhibitors of lipoxygenase and cyclooxygenase may not only inhibit the production of inflammatory substances, but also inhibit the cell membrane destruction by free radicals produced during inflammation and the peroxidation reaction of lipids, and may exhibit anti-inflammatory action. In addition, by inhibiting proteins and lipolytic enzymes produced by excessive inflammation, it is possible to suppress skin aging by protecting damaged cells.

In addition, atopic skin occurred mainly in the early childhood, and was also called taeyeol. It occurs mainly as the content of ceramides in the stratum corneum decreases. As the moisture retention function of the stratum corneum decreases, the skin dries and easily occurs and the skin barrier ( As the skin barrier function decreases, the irritant-inducing substance penetrates into the stratum corneum to induce irritation, and as the antigen protein, which is difficult to invade in the normal skin, penetrates easily, skin allergies and the like are easily generated and show symptoms of pruritus. Therefore, more than 90% of atopic patients are infected with Staphylococcus aureus, which is caused by scratching because the patient can't tolerate itching, but recent reports indicate that the exotoxin from this bacterium stimulates the body's immune system and causes allergies. It is said that it exacerbates atopy by making it come out. In other words, the bacteria themselves act as allergens.

The causes of atopic skin have been largely identified so far. First, they are caused by genetic causes, and are composed of phospholipids of the cell membrane, which is a skin protective layer responsible for skin protection, and intercellular lipid, which is a binding substance between keratinocytes. The lack of the essential fatty acid gamma-linoleic acid (γ-linoleic acid; GLA) does not make the skin protective film hard, so the skin surface easily loses moisture and is easily allergic by external stimulation. It is caused by environmental factors. As the living conditions of apartments become universal due to changes in the living environment of modern people, atopy skin is increasing worldwide as the living environment changes to a dry state. As a result, water retention decreases and skin protection function is lost, resulting in less irritation. Allergies occur by reacting sensitively to Edo, the third is caused by free radicals, and lipid peroxide is produced by free radicals, which causes atopic skin, and fourthly, infections caused by bacteria, viruses, and fungi. As a result, it is considered that these causes occur in a complex relationship (Dermatology, Korean Society of Dermatology, Publication Committee, Yeomungak, 1992).

Korean Patent Publication No. 10-2014-0042886 discloses a prior art related to anti-inflammatory and anti-allergic, which relates to an anti-inflammatory steroid condensed at 16,17 positions having a pyrrolidine ring. Compounds of glucocorticosteroids having anti-inflammatory and anti-allergic action, methods for preparing these compounds, pharmaceutical compositions, combinations thereof, and their therapeutic uses, and more specifically, to the pyrrolidine derivative glucocorticosteroid It is about.

The prior art is to prepare a compound through artificial organic synthesis in order to obtain the efficacy of anti-inflammatory and anti-allergic. It is difficult to obtain a practical effect due to poor stability during synthesis, and there is a problem in that side effects are generated by the compound depending on the person. .

That is, in order to reduce skin irritation and inflammation caused by various stress sources and reduce side effects due to the use of cosmetics, it is urgently required to develop a substance having anti-inflammatory, anti-allergic and skin irritation-reducing effects, along with side effects on the human body There is also a need for a method that can effectively extract these components while minimizing the destruction of the components of useful components of natural products.

Therefore, in order to improve the anti-inflammatory, anti-allergic and atopy skin, the effect of improving the anti-inflammatory, anti-allergic and atopy skin is excellent, and there is no side effect and the development of an inexpensive substance and the development of an easy-to-use cosmetic containing the substance This is essential.

Accordingly, the present invention was devised to solve the above problems, and extracts black bellflower, black donkey, and black ginger obtained by steaming bellflower and Angelica with steam 9 times and drying them 9 times (9 diarrhea 9 packs) by ultrasound. , Anti-inflammatory, anti-allergic and atopy skin improving effects to eliminate free radicals in cells and inhibit the activity of lipid peroxidation, lipoxygenase, hyaluronidase by providing a lyophilized mixed extract, and The purpose is to provide a manufacturing method.

In addition, the present invention improves skin inflammation, allergies and atopic skin by providing a cosmetic composition containing a mixture of black bellflower, black Angelica, and black ginger extracted with 9 and 9 envelopes of bellflower and Angelica, improving side effects on skin irritation and skin irritation An object of the present invention is to provide a cosmetic composition containing an extract having anti-inflammatory, anti-allergic and atopic skin-improving effects.

Accordingly, the present invention was devised to solve the above problems, and extracts black bellflower, black donkey, and black ginger obtained by steaming bellflower and Angelica with steam 9 times and drying them 9 times (9 diarrhea 9 packs) by ultrasound. , Anti-inflammatory, anti-allergic and atopy skin improving effect to provide freeze-dried mixed extract to inhibit free radical scavenging in cells and inhibit lipid peroxidation, lipoxygenase, and hyaluronidase activity, and The purpose is to provide a manufacturing method.

In addition, the present invention improves skin inflammation, allergies and atopic skin by providing a cosmetic composition containing a mixture of black bellflower, black Angelica, and black ginger extracted with 9 and 9 envelopes of bellflower and Angelica, improving side effects on skin irritation and skin irritation An object of the present invention is to provide a cosmetic composition containing an extract having anti-inflammatory, anti-allergic and atopic skin-improving effects.

Anti-inflammatory, anti-allergic and atopy skin improvement effect according to the present invention as described above, black bellflower, black Angelica, black ginger mixed extract and its manufacturing method is bellflower, black bellflower that has undergone 9 diarrhea and 9 processes of steaming and drying from Angelica , Black Angelica and Black Ginger are extracted, concentrated, and freeze-dried to provide black bellflower, Black Angelica, and Black Ginger powder, thereby free radical scavenging in cells, lipid peroxidation, lipoxygenase, hyaluronia It has the effect of suppressing the activity of the aze.

In addition, the cosmetic composition containing a mixed extract of black bellflower, black angelica, and black ginger with anti-inflammatory, anti-allergic and atopy skin improvement according to the present invention provides a cosmetic composition containing black bellflower, black angelica, and black ginger mixed extract By doing so, it has the effect of improving skin inflammation, allergies and atopic skin, and preventing side effects on skin irritation.

1 is a flow chart of a method of manufacturing a black bellflower, black angelica, and black ginger mixed extract having anti-inflammatory, anti-allergic and atopy skin improvement according to the present invention.

The present invention is an anti-inflammatory, anti-allergic and atopy skin search for various natural substances in order to find a basic substance that is effective, as a result, it is very effective against anti-inflammatory, anti-allergic and atopy skin improvement in bellflower, Angelica and ginger It was discovered that this was followed by the process of steaming and drying through 9 steaming and 9 foaming, and simultaneously solving the stability and safety issues of the products applied with the mixed extract extracted with ultrasonic waves.

In addition, it was found that even in a result of experiments applied directly to human skin by blending a certain concentration or more in a cosmetic composition, a distinct skin condition was exhibited.

Hereinafter, the present invention will be described in detail.

The present invention is an anti-inflammatory, anti-allergy and atopy skin improvement effect of black bellflower, black angelica, and black ginger mixed extracts of 9 bells and 9 bags through steaming and drying of bellflower and Angelica. Gives

The above bellflower ( Platycodon grandiflorum A. De Candolle ) is a perennial herb belonging to the family Campanulaceae and is mainly distributed in East Asia such as Korea, China, Japan, Taiwan, and Russia. The root of the bellflower (Kilkyung) is used for food, and is widely used as a herbal medicine since ancient times in Donguibogam or Bonchogangmok. Especially, it is useful as expectorant, pear, antitussive, tonsillitis, choeyu, cold, cough, asthma, tuberculosis, expectorant It is said to be effective against pleurisy. In addition, it contains platicodin, saponin, glaticodin, and spinasterol, and has anti-inflammatory, antioxidant, and atopic dermatitis inhibitory effects (Jung Seung-il et al., Korea Food and Nutrition Science Society, 2013, 42(5), p759-765 ).

The Angelica gigas Nakai is a perennial herb belonging to the Apiaceae family and is well-known as a succulent herb belonging to the genus Apiaceae. Mainly in the late autumn the following spring, the roots are pruned before spring buds are sprouted, the leaves/stems are removed, washed with clean water, and then dried in a shaded place where the wind is well used for medicinal purposes. In particular, it is well known as an effective medicine for women, and Korean Angelica, which is called a native Angelica, contains a large amount of decursin, decrusinol, and modakenin ingredients, so it has various pharmacological effects such as anti-inflammatory, analgesic, immune, liver function strengthening, and sedation. (Lee Jae-jun et al., Korean Food Storage and Distribution Society, 2009, 16(1), p94-100).

The ginger (Zingiberis Rhizoma) is the root of the ginger (Zingiber officinale), a perennial plant of the ginger family (Zingiberace), which is effective in indigestion, vomiting, diarrhea, and has pharmacologically high effects such as promoting blood circulation. . In particular, since ginger contains 6-gingerol, it has been reported to have strong antioxidant and antibacterial, anti-inflammatory, and dermatitis-inducing and therapeutic effects (Yang Jae-heon et al., Korean Pharmaceutical Association, 2006, 36(4), p253-262). .

The black bellflower, Angelica mixed extract prepared by using the bellflower, Angelica, and ginger, or the mixed extract obtained by mixing and extracting the Black bellflower, Black Angelica, and black ginger through ultrasound, free radical scavenging in cells, lipid peroxidation, and lipoxygenase , Hyaluronidase inhibitory effect is excellent.

A method of preparing a mixed extract of black bellflower, black Angelica, and black ginger using the bellflower, Angelica, and ginger will be described with reference to the accompanying drawings.

1 is a flow chart of a method of manufacturing a black bellflower, black angelica, and black ginger mixed extract having anti-inflammatory, anti-allergic and atopy skin improvement according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, and the method of manufacturing the extract of black bellflower, black donkey and black ginger with anti-inflammatory, anti-allergic and atopy skin improvement effects of the present invention includes the following steps. It is preferred, but is not limited to this.

The present invention repeats the process of steaming the washed bellflower, Angelica and ginger, and the process of drying the steamed Bellflower, Angelica and Ginger. The step of extracting ultrasonic or hot water by mixing a black bellflower, black donkey, and black ginger that have undergone the drying process at a predetermined rate, and concentrating the mixed extract after the extraction process under reduced pressure to obtain a black bellflower, black donkey, and black ginger mixed extract concentrate. Black having anti-inflammatory, anti-allergic and atopy skin improvement effect, comprising the steps of freeze-drying the black bellflower, black Angelica, and black ginger mixture extract to obtain a black bellflower, black Angelica, and black ginger concentrate. It is characterized in that it provides a cosmetic composition containing a mixed extract of black bellflower, black angelica, and black ginger prepared by the method of manufacturing the bellflower, black angelica, and black ginger mixture.

When explaining the manufacturing method of the present invention in each step is as follows.

The step (S10) of obtaining black bellflower, black donkey, and black ginger is performed through 9 distillation and 9 bags that repeat drying and drying of bellflower, donkey and ginger.

The bellflower, Angelica and ginger are washed several times with water, and each of the bellflower, Angelica and ginger is steamed, respectively. .

Specifically, the bellflower, Angelica and ginger washed in the process of 9 and 9 bags were steamed for 8 to 16 hours at a temperature of 65 to 90°C, respectively. The drying process is performed for 8 to 48 hours at a temperature of 30 to 50°C.

The above washing and drying process of steamed bellflower, Angelica and ginger is repeated 9 to 15 times.

If the steaming process is carried out for a long time exceeding 90 to 96°C outside the above range, active ingredients such as saponins or pradicodines contained in bellflower may be destroyed, and when the steaming is performed at a temperature higher than 120°C Its value is lowered as a product because its own organization becomes flabby. In addition, the temperature and time above are in the form of bellflower, Angelica, and ginger because they are not mature enough to proceed to the steaming at a temperature lower than 65°C or the difficulty in removing the flavor and taste of the bellflower root itself. This is the optimal range to obtain the desired black bellflower, black donkey and black ginger.

Next, the black bellflower, black donkey, and black ginger obtained through the above 9 symptom 9 packs are subjected to a step (S20) of extracting black bellflower, black donkey, and black ginger mixture by ultrasonic or hot water extraction again.

In detail, a black bellflower, black donkey, and black ginger are mixed at a specific ratio with a wavelength of 10 to 70 KHz and a output of 50 to 1,000 watts from the ultrasonic extractor to extract them for 4 to 12 hours at 60 to 80°C. Perform.

The wavelength and output range is an optimal range that prevents sediment from being generated by crushing the structure of the black bellflower and Angelica, and the temperature range can maintain the shape without boiling the black bellflower and Angelica and optimally contain the contained active ingredients. It is the range that can be eluted.

In addition, the wavelength, output, and temperature ranges are optimal in that the effect of inhibiting the activity of free radical scavenging in cells from black bellflower, black donkey and black ginger, and inhibiting the activity of lipid peroxidation, lipoxygenase, and hyaluronidase is excellent. Range.

The extraction solvent is preferably a mixed solvent selected from one or more of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin and ethylene glycol.

Another extraction method is a hot water extraction method in which a mixture of black bellflower, black donkey and black ginger obtained through the above 9 steaming 9 bags is added to a extractor equipped with a cooling condenser, and an extraction solvent is added to black bellflower, Angelica A mixed extract is obtained.

Specifically, in order to prevent the solvent from evaporating, black bellflower, Angelica and extracting solvent are added to an extractor equipped with a cooling condenser, and heated to extract at 60 to 80° C. for 4 to 24 hours.

The temperature range during hot water extraction is an optimal range in which free radical scavenging is performed from black bellflower, black donkey and black ginger, and the effect of inhibiting the activity of lipid peroxidation, lipoxygenase, and hyaluronidase is excellent.

The extraction solvent is an extraction solvent in which one or more mixtures selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin and ethylene glycol are used, black bellflower, black donkey, black It is preferred to add an amount of 1 to 20 times the weight of ginger.

In another extraction method, the black bellflower, black angelica, and black ginger obtained through the above 9 diarrhea 9 bags are added to the immersion machine in a uniform ratio, and water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin and ethylene The active ingredient is extracted by immersion for 1 to 7 days at 5 to 30°C using an extraction solvent in which one or more mixtures selected from the group consisting of cliccol are mixed.

Next, a step (S30) of obtaining a mixture of black bellflower, black Angelica, and black ginger by extracting the black bellflower, black Angelica, and black ginger mixture under reduced pressure is performed (S30).

The black bellflower, black angelica, and black ginger mixture extracts obtained in the extraction step (S20) are put in a vacuum concentrator and concentrated at 30 to 50° C. to obtain a black bellflower, black angelica, and black ginger mixed extract concentrate.

Next, a step (S40) of obtaining a mixture of black bellflower, black Angelica, and black ginger by performing freeze-drying of the concentrated extract of black bellflower, black Angelica, and black ginger (S40).

The concentrated extract of black bellflower, black donkey, and black ginger obtained in the reduced-pressure concentration step (S30) is freeze-dried and then pulverized to obtain a black bellflower, black donkey, and black ginger concentrated powder.

The anti-inflammatory, anti-allergic and atopy skin improvement effect according to the present invention, black bellflower, black Angelica, black ginger mixed extract improves skin inflammation, allergy and atopy skin, has no skin toxicity, can be applied to sensitive skin, It can be used in various cosmetic compositions because there is no skin side effect even when used for a long time.

In addition, the cosmetic composition containing a mixed extract of black bellflower, black Angelica and black ginger, which has anti-inflammatory, anti-allergic and atopy skin improvement effects according to the present invention, free radical scavenging effect in cells, lipid peroxidation, hyaluronidase activity inhibition, It has excellent anti-oxidation, anti-inflammatory, and skin improvement effects such as inhibitory activity of lipoxygenase, and is a safe, low-cost, and easy-to-use cosmetic with no side effects in skin irritation tests. It is a cosmetic composition excellent in ease.

That is, the black bellflower, black Angelica, black ginger mixture extract may be prepared in an emulsifying type, solubilizing type, oily, powder dispersed type, or solid powdery form to be applied as a cosmetic. Specifically, the cosmetic composition can be applied as a lotion, skin, lotion, cream, foundation, essence, gel, pack, etc., more specifically, emulsifying sunscreen cream, emulsifying foundation, emulsifying makeup base, oil It can be applied as a cake-type foundation, two-way cake, or powder pact.

In the cosmetic composition, the anti-inflammatory, anti-allergic and atopy skin improvement effect according to the present invention, black bellflower, black Angelica, black ginger mixed extract may contain 0.1 to 20% by weight based on the total liquid weight of the cosmetic composition, While recovering the evaporated solvent using a distillation apparatus equipped with a cooling condenser, the dry weight may include 0.001 to 5% by weight, preferably 0.01 to 3% by weight.

Hereinafter, it will be described in detail by examples, comparative examples, test examples and production examples of the present invention.

The following examples, comparative examples, test examples, and manufacturing examples are merely illustrative of the present invention, and the present invention is not limited to the following examples, comparative examples, test examples, and manufacturing examples.

<Example 1> Black bellflower, black Angelica, black ginger mixed extract and powder manufacturing method

After washing the collected bellflower and Angelica water several times in running water to remove soil and harmful substances sufficiently, remove the dried bellflower, Angelica and ginger from the steamer under conditions of 90℃ and 1.3 atm 12 respectively. After steaming for a period of time and drying the hot air at 50℃ for 18 hours, 9 kg of black bellflower, 0.9 kg of black donkey, and 0.6 kg of black ginger (total weight of 3.0 kg) were added to 20 kg of 30% ethyl alcohol. Put it and extract for 4 hours at 50 ℃ with an ultrasonic extractor (wavelength: 50 KHz, output 800 watt), and filtered with a 300 mesh filter cloth, 1 micro, 0.45 micro filter. Then, the concentrated extract of black bellflower, black Angelica, and black ginger concentrated at 50°C using a vacuum concentrator (buchi) was freeze-dried using a freeze dryer (Ilshin Lab) to obtain a dry weight of 498.5 g.

< Example 2~7> Black bellflower , Black donkey , Black ginger Mixed extract and powder manufacturing method

In the same manner as in Example 1, the samples in Table 1 below (black bellflower, black donkey, black ginger) were extracted at a mixed ratio to obtain an extract dried as shown in Table 1 below.

Black bellflower: Black Angelica: Black ginger consumption (Kg) Total consumption (Kg) Yield (g) Example 2 2.0: 0.5: 0.5 3 476.3 Example 3 0.5: 2.0: 0.5 3 485.7 Example 4 0.5: 0.5: 2.0 3 472.7 Example 5 1.5: 0.75: 0.75 3 479.6 Example 6 0.75: 1.5: 0.75 3 483.2 Example 7 0.75: 0.75: 1.5 3 477.5

< Preparation Example 1> Preparation of a cosmetic lotion containing a mixed extract of black bellflower , black angelica , and black ginger with anti-inflammatory, anti-allergic and atopy skin improvement effects according to the present invention

The prescription examples of the cosmetic lotion (skin lotion) in the cosmetic composition containing the mixed extract powder of bellflower, Angelica and ginger 9 Example 9 in Example 1 are shown in Table 2.

number Raw material Content (% by weight) One Example 1 0.1 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene cured castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Coloring a very small amount 10 Spices a very small amount 11 Purified water Balance

<Manufacturing method>

After adding 2, 3, 4, and 8 in order and dissolving by stirring, 5 was dissolved by heating to about 60°C, and after 10 was dissolved, it was added to 11. Lastly, 6, 7, and 9 were added, and 1 was added to sufficiently stir and aged.

< Preparation Example 2> Preparation of a lotion containing a mixed extract of black bellflower , black donkey and black ginger with anti-inflammatory, anti-allergic and atopy skin improvement effects according to the present invention

Table 1 shows a prescription example of a nutritional lotion in a cosmetic composition containing a mixed extract powder of bellflower, Angelica, and ginger 9, 9 bags of Example 1.

number Raw material Content (% by weight) One Example 1 0.1 2 Wax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Floating paraffin 10.0 6 Sorbitan stearate 1.0 7 Lipophilic glycerin monostearate 0.5 8 Stearic acid 1.5 9 Glyceryl stearate/page-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Coloring a very small amount 15 Spices a very small amount 16 Purified water Balance

<Manufacturing method>

10, 11, 13, 16 are mixed and stirred, heated to between 80 and 85°C, put into the manufacturing unit, and then acted as emulsifiers, and 2, 3, 4, 5, 6, 7, 8, 9, and 12 are 80 to 85°C. After heating and dissolving, emulsify. After the emulsification is completed, the mixture is cooled to 50°C with stirring using a stirrer, and then 15 times, after cooling to 45°C, 15 times, and 1 time at 35°C is cooled to 25°C and aged.

< Preparation Example 3, Comparative Example 1> Preparation of a cream containing a mixed extract of black bellflower , black angelica and black ginger with anti-inflammatory, anti-allergic and atopy skin improvement effects according to the present invention

The prescription example of the nutritional cream in the cosmetic composition containing the mixed extract powder of bellflower, Angelica, and ginger 9 Example 9 in Example 1 is shown in Preparation Example 3 of Table 4.

number Raw material Preparation Example 3
Content (% by weight) Comparative Example 1
Content (% by weight) One Example 1 0.1 - 2 Stearic acid 2.0 2.0 3 Cetyl alcohol 2.0 2.0 4 Glyceryl monostearate 2.0 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 0.5 6 Sorbitan sesquioleate 0.5 0.5 7 Glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate 1.0 1.0 8 Wax 1.0 1.0 9 Floating paraffin 4.0 4.0 10 Squalane 4.0 4.0 11 Caprylic/Capric Triglyceride 4.0 4.0 12 Carboxyvinyl polymer 0.3 0.3 13 Butylene glycol 5.0 5.0 14 glycerin 3.0 3.0 15 Triethanolamine 0.5 0.5 16 Purified water Balance Balance

<Manufacturing method>

After mixing 12, 13, 14, and 16 with mixing and stirring, the mixture is heated to 80 to 85° C., and then added to the manufacturing unit to act as an emulsifier and 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11 are 80 to After heating to 85° C. to dissolve, 15 was added and stirred, and the mixture was added to the production section and emulsified. After the emulsification is finished, the mixture is cooled to 35°C with stirring using a stirrer, cooled to 25°C by adding No. 1 and aged.

< Preparation Example 4> Preparation of an essence containing an extract of black bellflower, black angelica, and black ginger with anti-inflammatory, anti-allergic and atopy skin improvement effects according to the present invention

Table 1 shows a prescription example of an essence in a cosmetic composition containing a mixed extract powder of bellflower, Angelica, and ginger 9, 9 bags of Example 1.

number Raw material Content (% by weight) One Example 1 0.1 2 Citosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steareth-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Shotgun 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Balance

<Manufacturing method>

2, 3, 4, 5 and 6 are homogenized at a constant temperature to be referred to as nonionic amphiphilic lipids. The nonionic amphiphilic lipid was mixed with 7, 8 and 14, but was mixed by adding No. 1, homogenized at a constant temperature, passed through a microfluidizer, and then 9 was added slowly at a constant temperature to homogenize, and then the micro Pass it through the fluidizer again and disperse it by adding 10, 11, 12, 13 to stabilize and age it.

< Test example 1> Evaluation of cell non-toxicity and cell proliferation effect

The cell proliferation effect using cell culture was measured for the extracts and dry powders prepared in Examples 1 to 7 above.

1) Experiment method

Cytotoxicity and proliferation experiments were conducted in the following manner. Cells being cultured (fibroblast, 3T3 cells) are dispensed into 96-well microplates at 5,000 cells/well, incubated in a thermostat for 30 minutes, and samples are administered at 50, 100, 200, and 400 (μg/ml) for each concentration. And incubated for 72 hours. And thiazoline blue was administered and further cultured for 4 hours. The culture medium was discarded, and the reaction stopper was added to each well of the microplate, stirred for 5 minutes, and absorbance was measured at 570 nm. As a control group, 10% fetal bovine serum (FBS) medium was administered at the same time as the sample injection amount, and co-culture was performed as an optimal condition for cell growth. .

2) Experiment result

The cell proliferation effect was calculated by the calculation formula 1, and the results are shown in Table 6 below.

[Calculation formula 1]

density
(ug/ml) Cell proliferation effect (%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Control 100 100 100 100 100 100 100 50 108.7 100.9 103.5 100.6 100.5 102.1 103.1 100 116.7 102.1 107.2 101.2 101.6 105.1 105.5 200 123.3 104.4 111.2 104.2 103.3 108.9 109.2 400 129.1 111.6 119.7 110.4 109.6 114.3 115.1

It was confirmed that the extracts of Examples 1 to 7 all had cell proliferation effects.

Among them, in the case of Example 1, the cell extracts obtained by mixing samples of 9 cells of 9 bellflower, 9 capsules of 9 Angelica 9, and 9 cells of ginger 9 at the same ratio and compared with the extracts of Comparative Examples 2-7 were extracted. It was confirmed that the effect.

< Test example 2> Free radical ( free radical ) Elimination effect evaluation

The free radical scavenging effect of the extracted and dried powders prepared in Examples 1 to 7 was measured.

1) Experiment method

The experiment was performed using the DPPH (1,1-diphenyl-2 picryl-hydrazyl) method (reference: Blois.MSNature 181, 1190, 1958), and DPPH and Quercetin were used by Sigma. . To 1 ml of 0.2 mM DPPH methanol solution, add 2 ml of ethanol or methanol solution of Examples 1 to 6 at various concentrations (5, 10, 20, 40, 80 μg/ml), stir well, and react for 10 minutes at room temperature. . Then, absorbance was measured at 517 nm, and purified water was used instead of each sample in a blank test.

2) Experiment result

The free radical scavenging effect was obtained using the following Equation 2 and the results are shown in Table 7 below.

[Calculation formula 2]

Concentration (ug/ml) Free radical scavenging effect (%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 0 0 0 0 0 0 0 0 5 25.9 19.3 11.7 19.1 11.6 13.5 17.1 10 53.7 43.7 24.2 32.5 20.4 23.5 29.5 20 84.1 61.2 40.8 53.7 39.7 44.1 55.7 40 97.6 85.3 79.6 86.8 75.6 80.5 86.2 80 97.7 94.8 96.5 92.1 95.1 97.6 SC50 14.03 19.00 24.58 20.55 25.98 23.93 20.82

As shown in Table 7, the free radical 50% scavenging effect of the extract powder of Example 1 is 14.03, and the free radical 50% scavenging effect concentration (IC ₅₀ ) of Examples 2 to 7 is 19.00, 24.58, 20.55, 25.98, It was confirmed that the effect is significantly superior to 23.93, 20.82 μg / ml.

< Test example 3> lipid peroxidation ( Lipid peroxydation ) Evaluation of inhibitory effect

Lipid peroxydation inhibitory effects were measured on the extracts and dry powders prepared in Examples 1 to 7 above.

1) Experiment method

After dissolving linolenic acid to 0.1% in 8% sodium lauryl sulfate aqueous solution, take 3.9 ml and add 0.1 ml of the sample according to concentration (56.25, 112.5, 225, 450, 900 μg/ml), and then irradiate with ultraviolet light for 1 hour. Investigate. Then, 1 ml was taken, 1.5 ml of 0.8% TBA aqueous solution and 1.5 ml of 20% acetic acid (pH 3.5) were added, cooled by bathing for 1 hour, and then 1 ml of purified water and 5 ml of n-BuOH:Pyridine (15:1) were added. After shaking and centrifugation, the n-BuOH layer was taken and absorbance was measured at 532 nm.

2) Experiment result

In the blank test, no sample was added. The lipid peroxidation effect was obtained using the following equation 3 and the results are shown in Table 8 below.

[Calculation formula 3]

Concentration (ug/ml) Lipid peroxidation inhibitory effect (%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 0 0 0 0 0 0 0 0 56.25 5.8 3.2 1.8 1.9 1.5 2.2 1.9 112.5 15.3 9.6 4.3 8.2 2.9 6.5 4.8 225 36.7 24.3 12.1 28.3 9.9 18.7 13.9 450 77.5 51.8 48.3 53.2 43.1 45.2 39.8 900 96.7 90.9 85.7 91.3 86.3 88.9 78.7 IC50 390.72 482.97 533.89 475.65 547.87 516.49 585.19

As shown in Table 8, Example 1 is a 390.72 μg/ml that is superior to lipid peroxidation 50% inhibitory concentration (IC ₅₀ ) of Examples 2 to 7 482.97, 533.89, 475.65, 547.87, 516.49, 585.19 μg/ml. It was confirmed that it has an excellent lipid peroxidation effect.

< Test example 4> Lipoxygenase ( Lpoxygenase ) Evaluation of inhibitory effect

The effect of inhibiting the activity of lipoxygenase (Lpoxygenase) on the extracted and dried powders prepared in Examples 1 to 7 was measured.

1) Experiment method

Experiments were performed using the TBARS method. Linoleic acid, Lipoxygenase, Thiobabitulic acid, and Quercetin used in the experiment were used by Sigma. To 1 ml of 1 mM Linoleic acid, 0.05 ml of Examples 1 to 6 at various concentrations (6.25, 12.5, 25, 50 μg/ml) was added, 0.95 ml of Lipoxygenase was administered, stirred well, and reacted at 25° C. for 10 minutes. . Then, 0.5 ml of Trichloroacetic acid was added and 1 ml of Thiobarbiturlic acid was added, followed by heating for 10 minutes and cooling in ice water for 2-3 minutes to complete the reaction. After the reaction was completed, 2 ml of Butanol was added to the reaction solution, and centrifugation was performed at 4,000 g for 5 minutes, and absorbance was measured at 535 nm. In this case, purified water was used instead of each sample as a blank test.

2) Experiment result

Lipoxygenase activity inhibitory effect was obtained using the following calculation formula 4 and the results are shown in Table 9 below.

[Calculation formula 4]

Concentration (ug/ml) Lipoxygenase activity inhibitory effect (%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 0 0 0 0 0 0 0 0 6.25 10.7 5.7 5.2 7.2 4.9 4.1 6.8 12.5 31.5 13.5 12.1 15.1 11.9 9.8 15.3 25 74.9 35.7 37.3 39.2 31.1 24.3 35.6 50 93.1 85.8 85.7 84.7 81.5 80.1 81.9 IC50 22.83 31.18 31.12 30.73 33.21 34.94 32.01

As shown in Table 9, the lipoxygenase 50% activity inhibition concentration of Example 1 (IC ₅₀ ) was 22.83 μg/ml, and the lipoxygenase 50% activity inhibition concentration of Examples 2 to 7 (IC ₅₀ ) 31.18, 31.12 , 30.73, 33.21, 34.94, and 32.01 μg/ml.

< Test example 5> Hyaluronidase Active inhibition Effect evaluation

The inhibitory effect of hyaluronidase activity was measured on the extracts and dry powders prepared in Examples 1 to 7 above.

1) Experiment method

It is a method of applying the Morgan-Elson method. The final enzyme activity of Hyaluronidase is 400NF unit/ml, the final concentration of HA is 0.4 mg/ml, and the inhibitory effect of the activation step of the inactive Hyaluronidase is inhibited by using the active compound 48/80 buffer solution (0.1 mg/ml). Hyaluronidase activity was measured centrally. The sample was dissolved in a buffer to prepare a sample solution, and a control group was used instead of the sample solution.

In addition, a buffer was used instead of the enzyme solution for each blank, and green tea extract was used as a control.

2) Experiment result

The effect of inhibiting hyaluronidase activity was calculated using the following formula 5, and the results are shown in Table 10 below.

[Calculation formula 5]

Concentration (ug/ml) Hyaluronidase inhibitory effect (%) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 0 0 0 0 0 0 0 56.25 23.1 18.2 15.2 15.6 10.9 9.1 11.3 112.5 48.3 41.2 29.7 34.3 24.7 22.1 25.7 225 79.7 69.7 63.2 55.7 51.6 49.2 52.9 450 95.4 90.2 88.9 90.2 83.5 81.7 86.6 900 97.2 97.8 98.9 97.5 96.7 95.6 IC50 172.16 263.23 298.79 298.88 339.24 353.34 242.07

As shown in Table 10, the hyaluronidase 50% activity inhibition concentration of Example 1 (IC ₅₀ ) is 172.16 μg/ml, and the hyaluronidase 50% activity inhibition concentration of Examples 2 to 7 (IC ₅₀ ) 263.23, It was confirmed that it was superior to 298.79, 298.88, 339.24, 353.34, 242.07 μg/ml.

< Test example 6> Evaluation of atopy improvement effect

Clinical evaluation of the atopic skin improvement effect on the cosmetic of Preparation Example 3 was measured. In order to evaluate the improvement effect of the cosmetic preparation 3 on atopic skin, the following experiment was conducted on 20 adults with atopic skin.

The test sample was applied to the whole body by dividing it into right and left, respectively, by double-blinded test, but the use of other moisturizers that could affect the effect of the test sample was prohibited. The results after 1, 2, 3, and 4 weeks after application of the test sample were evaluated by SCORAD (SCORing Atopic Dermatitis) measurement method, and the results are shown in Table 11 below.

1) Criteria

① Extent criteria: area = damaged part/100

② Intensity criteria

-Erythema (1/2/3)

-Edema (1/2/3)

-Exudation (1/2/3)

-Skin peeling (1/2/3)

-Taesunhwa degree (1/2/3)

-Drying degree (1/2/3)

③ Subjective standards

-Itching (1-10)

-Insomnia (1-10)

* Calculation of Scorad = (Accuracy Criteria/5) + (Strength Criteria/7) + Subjective Criteria

Classification 0 weeks 1 week 2 weeks 3 weeks 4 weeks Preparation Example 3 65.8 36.2 18.2 13.6 8.2 0.00% 44.98% 72.34% 79.33% 87.54% Comparative Example 1 67.1 47.5 36.7 29.6 20.7 0.00% 29.21% 45.31% 55.89% 69.15%

As shown in Table 11, the cosmetic composition of Preparation Example 3 containing the bellflower Angelica 9 9 9 mixed extract (Example 1) is purified water as much as the Bellflower Angelica 9 9 9 mixed extract (Example 1) in Preparation Example 3 It can be seen that the atopy skin improvement effect is very excellent as compared with Comparative Example 1 to which was added. In particular, looking at the results after 4 weeks, it shows an excellent effect of 87.54% improvement based on the Scoread index.

On the other hand, in order to examine the effect of reducing the atopic dermatitis felt by the caregiver, after using the cosmetic preparations of Preparation Example 3 and Comparative Example 1 for 1 month, the user was concerned about itching, erythema change, and dry skin of the dermatitis according to the criteria in Table 12 below. It is shown in Table 13 below to evaluate the degree of improvement in overall dermatitis and the side effects of the test sample along with satisfaction.

One 2 3 4 5 no effect Some usually Great Very good Very complaining dissatisfaction usually Satisfaction very good

Classification Skin itching Erythema changes Dry skin Overall improvement effect Side Effect Preparation Example 3 4.2 3.9 3.9 4.3 4.1 Comparative Example 1 3.5 2.6 3.3 3.1 3.8

As shown in Table 13, the cosmetic preparation of Preparation Example 3 according to the present invention is superior to the comparative example 1 in which the extract of the present invention is not added in all the evaluation items for adults with atopic skin as the test subject. can confirm.

What has been described in the present invention is only an example for a mixed extract of a bellflower Angelica 9 Jeung 9 packet having an anti-inflammatory, anti-allergic and atopy skin improvement effect, a method for manufacturing the same, and a cosmetic composition thereof, the present invention being limited to the above examples However, it can be implemented in a variety of different forms, only this embodiment is provided to complete the disclosure of the present invention, and to fully inform the scope of the invention to those of ordinary skill in the art. And the invention is only defined by the scope of the claims.

Claims (12)

9 bellflowers, Angelica and ginger, respectively, are mixed in a weight ratio of 5:3:2 and combined with a weight ratio of 10:70 KHz, ultrasonic extraction at 60 to 80°C for 4 to 12 hours at a wavelength of 50 to 1,000 watts, or 60 It is characterized by inhibiting the activity of lipid radical peroxidation, lipoxygenase and hyaluronidase by erasing free radicals in cells by extracting hot water at 80 to 80°C for 4 to 12 hours, concentrating under reduced pressure at 30 to 50°C and freeze-drying. Anti-inflammatory, anti-allergic and atopy skin extracts of bellflower, Angelica and ginger with 9 and 9 packs.
delete According to claim 1,
The extraction is an anti-inflammatory, anti-allergic and atopy skin improving effect, characterized in that it is at least one extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin and ethylene glycol, 9 extracts of Angelica and ginger 9 packs.
Step 9 to secure the black bellflower, black Angelica and black ginger by 9 9 9 bellflower, Angelica and ginger (S10);
The black bellflower, black donkey and black ginger of the above step (S10) are mixed in a weight ratio of 5:3:2 to eliminate free radicals in the cells and inhibit the activity of lipid peroxidation, lipoxygenase and hyaluronidase. And a 10 to 70 KHz wavelength and 50 to 1,000 watt output at 60 to 80 ℃ ultrasonic extraction for 4 to 12 hours or 60 to 80 ℃ hot water extraction for 4 to 12 hours to obtain a mixed extract (S20);
Step (S30) to obtain a mixed extract concentrate by concentration under reduced pressure at 30 to 50 ℃ the mixed extract of the step (S20);
The step (S40) of freeze-drying the mixed extract concentrate of the step (S30) to obtain a concentrated extract powder (S40); anti-inflammatory, anti-allergy and atopy skin improvement effect of bellflower, Angelica and ginger 9 Method for preparing a mixed extract.
delete The method of claim 4,
In the step (S20), anti-inflammatory, characterized in that ultrasonic or hot water extraction is performed using one or more extraction solvents selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin and ethylene glycol, Method for preparing mixed extract of 9 bellflower, 9 Angelica and ginger with anti-allergic and atopy skin improvement effect.
delete delete delete Claim 1, including the bellflower, Angelica and ginger of 5: 3: 2 in a weight ratio of 9 to 9, containing a mixed extract of cells to eliminate free radicals in the cell and lipid peroxidation, lipoxygenase and hyaluronidase activity By inhibiting the anti-inflammatory, anti-allergy and atopic skin cosmetic composition containing a mixture extract of bellflower, Angelica and ginger 9 9 9 packs.
The method of claim 10,
The composition is a bellflower having anti-inflammatory, anti-allergic and atopy skin improvement effect, characterized in that it comprises 0.001 to 5% by weight of the total dry weight of the cosmetic composition of claim 9 of the bellflower, Angelica and ginger of claim 1 , Angelica and ginger 9 cosmetic composition containing 9 extracts.
The method of claim 10,
The cosmetic composition is a formulation of lotion, skin, lotion, cream, foundation, essence, gel, pack, emulsifying sunscreen cream, emulsifying foundation, emulsifying makeup base, oil cake foundation, two way cake, or powder pact A cosmetic composition containing a mixed extract of bellflower, Angelica and ginger 9, 9, having anti-inflammatory, anti-allergic, and atopy skin-improving effects, characterized by formulating.

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