KR20160003920A - Cosmetic composition for anti-inflammation or improving skin trouble containing herb extracts - Google Patents

Abstract

The present invention relates to a composition for anti-inflammation or trouble treatment comprising a herbal medicinal extract and, more specifically, to a cosmetic composition comprising one or more extracts or fractions of the same selected from the group consisting of Dioscorea japonica Thunb. root, Ledebouriella seseloides, Atractylodes macrocephala Koidzumi, Zingiber officinale, and Zizyphus jujuba Miller var. inermis Rehder as active components for anti-inflammation or trouble treatment.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for anti-inflammation or improving skin trouble containing herb extracts,

The present invention relates to a cosmetic composition for improving anti-inflammation or trouble containing an extract of a medicinal herb, and more particularly, to a cosmetic composition for improving the anti-inflammatory or trouble-improving cosmetic composition comprising an extract of a medicinal herb, And to a cosmetic composition for improving anti-inflammation or trouble.

Inflammation is the immune response of the body in response to a wound or disease. Oxidative stresses such as ultraviolet rays, active oxygen, and free radicals activate inflammatory factors and cause aging of various diseases and skin. The vasoactive polypeptide, kinin, plasmin and complement, have vasodilatory and contraction and chemotaxis effects, as well as lymphokines such as interleukin-6 (IL-6) Phosphorus and arachidonic acid are responsible for inflammation. Arachidonic acid is metabolized through inflammatory mediators such as prostaglandin and leukotrienes via two pathways: cyclooxygenase or lipooxygenase, mediating a variety of inflammatory responses.

On the other hand, a substance that acts to eliminate inflammation and to reduce vital signs and symptoms to eliminate inflammation is called an anti-inflammatory agent. To date, there have been a number of nonsteroidal anti-inflammatory drugs, including flufenamic acid, ibuprofen, benzydamine, indomethacin, prednisolone, , Dexamethasone, and the like. It is also known that allantoin, azene, hydrocortisone and the like are effective for antiinflammation. However, these materials are limited in their use due to safety of skin, .

Under these circumstances, the inventors of the present invention conducted studies on substances which are not toxic to naturally occurring natural resources and have excellent skin condition improving effect, and selected from the group consisting of yam root, licorice root, windblown, ginger and jujube And the composition containing at least one kind of herbal medicine extract as an active ingredient showed the NO production inhibitory effect, and the composition for improving anti-inflammation or trouble using the same was completed.

Korean Patent Publication No. 10-1309172 Korean Patent Registration No. 10-0954984

The object of the present invention is to provide a cosmetic composition for improving anti-inflammation or trouble, which comprises as an active ingredient at least one extract or a fraction thereof selected from the group consisting of yam root, licorice, windblown, alfalfa, ginger and jujube will be.

In order to solve the above problems, an embodiment of the present invention is characterized by comprising, as an active ingredient, at least one extract or a fraction thereof selected from the group consisting of yam root, licorice, windblown, Thereby providing a cosmetic composition for improving anti-inflammation or trouble.

In another embodiment of the present invention, there is provided a cosmetic composition for improving anti-inflammation or trouble, which comprises, as an active ingredient, a mixed extract of yam root, licorice, windshield, alfalfa, ginger and jujube or a fraction thereof.

In another embodiment of the present invention, the mixing ratio of yam root, licorice, windblown, alfalfa, ginger and jujube is 15 to 45:15 to 45: 5 to 15: 5 to 15: 5 to 15: To 15 days.

In the present invention, yam root means rootstock of yam (Dioscorea japonica) belonging to Dioscoreaceae. It is also used as a medicament such as goiter, carditis, detoxification, dryness, burn, frog, back pain, Preferably, the dried yam root can be used.

In the present invention, licorice (Glycyrrhiza glabra) means a perennial herb that belongs to leguminosae. Preferably, the root or stem of licorice root can be dried and used. Licorice harmonizes the toxicity of all medicines and makes the medicinal effect appear, regulates the fever and morale of the book, makes good communication of all blood vessels, and strengthens the muscles and bones. Pharmacological action is effective for detoxification, hepatitis, urticaria, and eczema. Jinhae, geomam, muscle relaxation, diuretic action and suppress peptic ulcer. Licorice crust is reddish brown or dark brown with vertical wrinkles and occasionally shrubs, buds and scales. The outer skin of the peeled licorice is pale yellow and fibrous.

In the present invention, Lepidoptera (Ledebouriella divaricata) refers to perennial herbaceous plants belonging to the genus Umbelliferae. Preferably, roots or rootstocks of windshields can be used by drying. Windshield is effective for all kinds of winds such as headache, chills, fever, whole body and sore throat. It is used for painful limb pain, tetanus, muscle cramps, paralysis due to paralysis, and paralysis. Pharmacological actions such as fever, swelling, immune activation, antiallergic, anti-ulcer, and antibacterial activity have been reported. The windshield is cylindrical and the lower part is slightly thinner. The outer surface is light brown, and the upper part of the rootstock has dense wrinkles with rounded nodules and some with a hairy sheath of brown hair. The roots have many vertical wrinkles and marks of thin roots. There are many fine hairs such as brushes on the head and radial pattern.

In the present invention, the term "flower bud" refers to the root stem of Atractylodes japonica belonging to Compositae. Preferably, the dried product may be dried. It is weak in the function of 脾 胃 (脾胃), news, mala appearance, yellow color of the face is diluted, the diarrhea is good, and the water is stagnant, the whole body is swollen, helps digestion of water when not digested. It can also be used for chest leaks, coughs, clear sputum due to accumulation of water and wetness in the spleen. 脾气 (脾气) When sweating on the skin spontaneously due to weakness, use. It is also used for cold and limb pain with gastrointestinal disorder. Pharmacological studies have been reported to increase mouse weight and endurance, increase phagocytosis, and stimulate cellular immune function. In addition, intestinal (intestinal) inhibition and control of excitement, anti-ulcer and liver function protection, immune function hyperactivity, vasodilation, diuretic and hypoglycemic effects, and anti-cancer effects have been reported.

In the present invention, ginger refers to the root of Zingiber officinale, a perennial plant belonging to Zingiberaceae. Preferably, the dried ginger can be used. Ginger treats chills, fever, headache, vomiting, seawater and sputum caused by colds. It also works on abdominal pain and diarrhea caused by food poisoning. It also boils ginger in boiling water and drinks in tea. Pharmacological action to promote gastric secretion, digestive power, cardiac excitement, blood circulation promotion, and bacillary action have been reported.

In the present invention, jujube means the mature fruit of the jujube (Zizyphus jujube) belonging to the Rhamnaceae family. Preferably, the jujube can be dried. Jujube is used for daily diets or for cooking such as rice cakes and short meals. Dried jujube in September, and it has efficacy such as nutrition, tonic, shinhae, analgesia, detoxification in one room, lack of energy, general pain, insomnia, muscle cramps, drug poisoning .

The term "extract" used in the present invention refers to an extract obtained by the extraction treatment of the yam root, licorice, windshield, alfalfa, ginger, jujube or mixture thereof, a diluted solution or an intensive solution of the extract, , An adjusted product of the above extract or a purified product thereof, or a mixture thereof, and an extract of all the formulations which can be formed using the extract itself.

The extract of the present invention can be extracted from natural, hybrid, or mutant plants of the respective plants, and can also be extracted from plant tissue cultures.

In the extraction of the yam root, licorice, windblown, liquor, ginger, jujube or mixture thereof according to the present invention, the extraction method is not particularly limited and may be carried out according to a method commonly used in the art . Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

In the present invention, the kind of the extraction solvent used to extract the yam root, licorice, windbreak, extract, ginger, jujube or mixture thereof is not particularly limited and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C ₁ to C ₄ lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof. Water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in admixture of two or more. In the present invention, water (or distilled water), 1,3-butylene glycol, or a mixed solvent thereof is preferably used as a solvent for extracting herbal medicine roots, licorice root, wind chimes, ginger, jujube or mixtures thereof And more preferably, a solvent in which water (or distilled water): 1,3-butylene glycol is mixed at a weight ratio of about 7: 3 can be used. More preferably, a solvent in which water (or distilled water): 1,3-butylene glycol is mixed at a weight ratio of about 6: 4 can be used. The solvent may be used to prepare a solvent extract by extracting the yam root, licorice, windblown, liquorice, ginger, jujube or a mixture thereof at least once. The solvent extract may be subjected to vacuum distillation or freeze drying or spray drying A dry extract can be prepared. In one specific embodiment of the present invention, a mixture of water (or distilled water) and 1,3-butylene glycol in a weight ratio of 6: 4 is used to make a yam root extract, licorice extract, windshield extract, And extracts of the herbal mixture were respectively prepared (Examples 1 to 7).

In the present invention, the extract obtained by hot water extraction or cold extraction is filtered to remove suspended solid particles by filtration, for example, nylon or the like, or by freeze filtration, Spray drying and the like.

The term "fraction " as used herein means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. As a non-limiting example of the above-mentioned fractionation method, there can be mentioned a method of obtaining a fraction from the extract by treating a predetermined solvent with an extract obtained by extracting yam root, licorice, windblown, extract, ginger, jujube or mixture thereof.

The kind of the solvent used for obtaining the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fraction solvent include polar solvents such as water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohols are used in the fraction solvent, C ₁ to C ₄ alcohols can be preferably used.

The yam root extract, licorice extract, windblown extract, extract of liquorice, ginger extract, jujube extract or mixture thereof of the present invention effectively inhibits the production of nitrogen monoxide (NO) to exhibit anti-inflammatory or anti-trophic effects. In addition, since the composition of the present invention is a substance derived from a natural material, it is not toxic and can be used as a cosmetic composition without adverse effect even when it is applied to a human body.

Inflammation reaction is a mechanism to regenerate damage due to physical action, harmful substance, chemical stimulation, bacterial infection as a part of defensive reaction of biotissue to external stimuli. Persistent inflammation causes rather mucosal damage and promotes damage, inflammation and skin troubles in skin tissue.

Macrophages play a crucial role in the immune response and control a variety of inflammatory mediators, including nitric oxide, prostaglandins, and inflammatory cytokines, the immune proteins. Among them, nitrogen monoxide plays an important role in killing bacteria or removing tumors, but excessive nitric oxide production by pathogen causes inflammation, and inflammation induces skin trouble.

In other words, inhibition of the production of mononuclear mononuclear nitric oxide can prevent, alleviate, ameliorate and treat skin inflammation and prevent, alleviate, improve and treat skin troubles caused by inflammatory reaction.

Specifically, in the case of using yam root extract, licorice extract, windshield extract, chrysanthemum extract, ginger extract, jujube extract or mixed extract thereof, the production of nitrogen monoxide in macrophages was higher than that in the case of using the basic medium (Example 8).

In particular, the extract of the mixture or its fractions containing both yam root, licorice, windblown, extract, ginger and jujube of the present invention can be obtained by synergistic or cooperative action between extracts and / In comparison with L-NMMA (L-NG-Monomethylarginine), which is a known inhibitor of nitrogen monoxide formation, it has been found that the inhibitory effect of monoethanolamine It was confirmed that there was a very potent inhibitory effect on the production of nitrogen monoxide, which was first identified in the present invention.

Specifically, the extract or the fraction thereof of the mixture containing yam root, licorice, windblown, alfalfa, ginger and jujube of the present invention is superior to the extract alone or fraction thereof of yam root, licorice, windbreak, Production inhibitory effect was further increased, and as a result, it was possible to exhibit a more excellent anti-inflammatory or skin-trouble-improving effect.

In the present invention, the blending ratio of yam root, licorice, windblown, alfalfa, ginger and jujube is preferably 15 to 45:15 to 45: 5 to 15: 5 to 15: 5 to 15: 5 to 15 have.

According to a specific embodiment of the present invention, a mixed extract having a mixing ratio of 30: 30: 10: 10: 10: 10 was prepared by mixing the yam root, licorice, windblown, As a result, it was confirmed that the degree of inhibition of nitrogen monoxide formation was larger than that of the single extract of yam root, licorice, windshield, alfalfa, ginger or jujube (Example 8).

The term "anti-inflammatory" as used in the present invention means all actions in which the immune response is alleviated and NO production is inhibited.

As used herein, the term "Trouble " means skin conditions such as skin rash, acne, pimples, and red nose which may be caused by inflammation caused by excessive nitric oxide production in macrophages.

The term "improvement of trouble" used in the present invention refers to inhibiting or inhibiting the generation of troubles in the skin, or alleviating the trouble already generated.

The cosmetic composition of the present invention can be used as a cosmetic composition in the form of a solution, a topical ointment, a cream, a foam, a nutritional lotion, a softening lotion, a pack, a soft water, a latex, a makeup base, But are not limited to, those selected from the group consisting of solutions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powdered foundations, emulsion foundations, wax foundations, patches and sprays no.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, use is made of an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, But is not limited thereto. These may be used alone or in combination of two or more.

In the cosmetic composition of the present invention, the herbal medicine extract of yam root, licorice, windblown, liquorice, ginger, jujube or mixture thereof may preferably be contained in an amount of 0.0001 to 50% by weight of the total weight of the cosmetic composition, And preferably from 0.0005 to 10% by weight. There is an advantage of exhibiting excellent anti-inflammatory or skin trouble-improving effect within the above-mentioned range, and there is an advantage that the formulation of the composition is stabilized.

The composition of the present invention containing the extracts of the yam root, licorice, windblown, liquorice, ginger, jujube or mixture thereof or fractions thereof is excellent in anti-inflammatory or skin-tear-improving effect. Specifically, the composition of the present invention is excellent in inhibiting the production of nitrogen monoxide in macrophages.

In addition, since the composition of the present invention is a substance derived from a natural substance, it has no toxicity and does not cause side effects even when it is applied to human body, and thus it is highly utilized as a cosmetic composition.

Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These embodiments are only for illustrating the present invention, and the scope of the present invention is not limited by these embodiments.

Example  One : Yam root extract  Produce

The yam roots were well dried and then placed in three separate flasks, and 100 g of dry weight was added to the flask and extracted with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 6: 4) for 3 days. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a yam root extract.

Example  2: Preparation of licorice extract

The licorice was well dried, and after three folds, the dry weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 6: 4) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a licorice extract.

Example  3: Preparation of windshield extract

After thoroughly drying the windshield, it was cut into three pieces, and 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 6: 4) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a windshield extract.

Example  4 : Extract  Produce

After thoroughly drying the extract, the dried weight of 100 g was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 6: 4) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a crude extract.

Example  5: Preparation of ginger extract

The ginger was well dried and cut into three pieces. 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 6: 4) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare ginger extract.

Example  6: Preparation of Jujube Extract

The jujube was well dried, and after finishing three times, 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 6: 4) for 3 days by cooling. The chilled extract was filtered with a filter having a pore size of 0.2 mu m to prepare jujube extract.

Example  7: Preparation of mixed extract of herbal medicines

(Yam root 30g, licorice 30g, wind, 10g, 10g, 10g ginger, 10g jujube) were added to the flask, and the mixture was extracted 1000 g of a solvent (distilled water: 1,3-butylene glycol = 6: 4) was extracted by cooling with ice for 3 days. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a herbal composition extract.

Example  8: Anti-inflammatory effect - NO  Production inhibitory effect

In order to confirm the anti-inflammatory effect and the skin trouble relieving effect of the herbal medicine extract, nitric oxide (NO) formation inhibition experiment was performed by the GRIESS method using the RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates at 3 × 10 ⁵ per well, and cultured for 24 hours. Subsequently, the herbal extract extract prepared in Examples 1 to 7 was diluted with the concentrations shown in the following Table 1 and replaced with the cell culture medium containing them. At this time, L-NMMA (L-NG-Monomethylarginine), a NO-production inhibitor, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) . 100 μl of the supernatant was transferred to a 96-well plate, and 100 μl of GRIESS solution was added thereto at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect of the herb extract. Respectively. The experiment was repeated three times.

Inhibitory Effect of Herb Medicine Extract on NO Production sample(%) NO production inhibition rate (%) Negative control group - L-NMMA (10 [mu] g / ml) 39.54 Example 1 (0.1%) 11.01 Example 1 (1.0%) 13.98 Example 2 (0.1%) 13.54 Example 2 (1.0%) 16.97 Example 3 (0.1%) 12.10 Example 3 (1.0%) 15.94 Example 4 (0.1%) 11.18 Example 4 (1.0%) 15.56 Example 5 (0.1%) 10.55 Example 5 (1.0%) 15.12 Example 6 (0.1%) 12.47 Example 6 (1.0%) 16.50 Example 7 (0.1%) 20.16 Example 7 (1.0%) 34.43

As can be seen from the results in Table 1, it can be confirmed that the concentration of 0.1% of the extracts of yam root extract, licorice extract, windshield extract, chrysanthemum extract, ginger extract, jujube extract, , It can be seen that the extract of the present invention can be used for anti-inflammation or trouble-improving purposes.

In particular, the 1% concentration of the mixed extract showed excellent NO production inhibitory effect similar to that of L-NMMA, which is known to be a NO production inhibitor. Therefore, it was found that the mixed extract of herb medicine prepared in Example 7 had a better anti-inflammatory or trouble-improving effect than the herbal extract alone.

Manufacturing example  1: Preparation of an essence containing the mixed extract of the present invention

A cosmetic composition of an essence formulation was prepared as shown in the following Table 2 (unit: wt%).

Raw material name Content (% by weight) Cetearyl alcohol
Cetearyl glucoside
Jojoba seed oil
Silica
glycerin
Example 7 Extract
Purified water 2
One
5
0.5
10
5
To 100

Manufacturing example  2: Production of a cream containing the mixed extract of the present invention

A cream form cosmetic composition was prepared as shown in Table 3 below (unit: wt%).

Composition Content (% by weight) Example 7 Extract
Stearic acid
ethanol
Potassium hydroxide
glycerin
Propylene glycol
antiseptic
incense
Purified water 5
15.0
1.0
0.7
5.0
3.0
Suitable amount
Suitable amount
to 100

Claims (7)

Wherein the extract comprises at least one extract or a fraction thereof selected from the group consisting of yam root, licorice, windshield, alfalfa, ginger and jujube as an active ingredient.
The cosmetic composition for improving anti-inflammatory or anti-inflammatory properties according to claim 1, which comprises, as an active ingredient, a mixed extract of yam root, licorice, windblown, extract, ginger and jujube or a fraction thereof.
The method according to claim 2, wherein the mixing ratio of the yam root, licorice, windblown, alfalfa, ginger and jujube is 15 to 45:15 to 45: 5 to 15: 5 to 15: 5 to 15: 5 to 15 Wherein the cosmetic composition is a cosmetic composition for improving anti-inflammation or trouble.
4. The cosmetic composition according to claim 3, wherein the mixing ratio of the yam root, licorice, windblown, alfalfa, ginger and jujube is 30: 30: 10: 10: 10: 10 by weight.
The method of claim 1 wherein the extract of yam root, licorice, wind, baekchul, ginger, and at least one member selected from the group consisting of jujube water, a lower alcohol of C ₁ to C _4, 1,3- butylene glycol, And ethyl acetate. The cosmetic composition for improving anti-inflammatory or anti-inflammatory properties according to claim 1,
6. The cosmetic composition according to claim 5, wherein the solvent is water and 1,3-butylene glycol at a weight ratio of 6: 4.
The cosmetic composition according to claim 1, wherein the anti-inflammatory or anti-inflammatory effect is due to inhibition of nitrogen monoxide (NO) production.