KR102606838B1 - Antioxidative and antiinflammatory compostion comprising the extract of plant root - Google Patents

Abstract

The present invention is a composition containing one or more of extracts of peony root, burdock root, Rehmannia glutinosa root, yam root, and Aspergillus root extract, and has excellent antioxidant and anti-inflammatory effects.

Description

ANTIOXIDATIVE AND ANTIINFLAMMATORY COMPOSTION COMPRISING THE EXTRACT OF PLANT ROOT}

The present invention relates to an antioxidant and anti-inflammatory composition containing plant root extract.

Skin has a primary function of protecting our body from the external environment and a cosmetic function.

Oxidative stress and inflammatory reactions in the skin are known to be the cause of various diseases that occur in the skin. Since oxidative stress and inflammatory reactions can cause various problems such as skin aging, irritation, pigmentation, decreased barrier function, and skin dryness, antioxidant and anti-inflammatory functions of the skin are the most basic necessities for healthy skin. There is. Oxidative stress and inflammatory reactions in the skin can be caused by various endogenous or exogenous factors, and are also easily caused by ultraviolet rays and fine dust that we can easily be exposed to in our daily lives.

Cosmetics are used for the aesthetic function of cleansing or beautifying the skin and the defensive function of protecting the skin from harmful environments.

However, in various external skin products used for the purpose of protecting the skin and beautifying and cleansing, the use of ingredients that are unavoidable but different from the purpose of skin protection, such as surfactants, preservatives, fragrances, sunscreens, pigments, and functional ingredients, is unavoidable. Raw materials cause various side effects on the skin. In particular, ingredients such as AHA (alpha hydroxy acid), retinol, and arbutin contained in functional products for whitening, wrinkle improvement, and exfoliation are the main causes of skin problems.

Oxygen, which is related to antioxidants, can easily acquire electrons depending on its electron configuration and can become a powerful oxidizing agent. Oxygen-related toxic substances in the human body are called reactive oxygen species (ROS). Types of these ROS include free radicals such as superoxide, hydroxyl, peroxyl, alkoxyl, and hydroperoxyl, and hydrogen peroxide ( There are non-free radicals such as hydrogenperoxide, ozone, singlet oxygen, and peroxinitrite.

Free radicals are not only continuously produced through the basic metabolic processes of cells (action of the electron transport system during the energy production process in mitochondria, phagocytosis of white blood cells, etc.), but also exist in the human body due to various causes such as ultraviolet rays, smoking, stress, and pollutants. I'm doing it.

In 1956, Denham Harman proposed a theory on aging and free radicals that states that free radicals produced during organic respiration accumulate oxidative damage, causing aging and death. Since then, highly reactive free radicals are closely related to more than 60 types of diseases, including heart disease, cancer, and cataracts, and have been revealed through various experiments to be an important factor in causing changes in cellular structure and function that are characteristic of aging. The theory that most of the physiological decline that characterizes age-related changes is caused by cellular damage caused by free radicals is increasingly gaining ground.

In order to protect the human body from such oxidative stress, much research is being conducted to isolate, identify, and develop natural antioxidants from edible or medicinal plants that are powerful and safe for human skin. However, when applied to actual products, they are easily oxidized due to various effects. There is a problem of discarding it, and even if the efficacy is increased by using antioxidant substances with various functions at the same time, there is a problem that the free radical scavenging ability is short-lived.

Meanwhile, inflammation is a mechanism that attempts to regenerate damaged areas that have changed due to stimulation within the body. A continuous inflammatory response promotes mucosal damage, resulting in pain, swelling, redness, and fever, resulting in functional impairment. Macrophages are known to be involved in maintaining homeostasis through various host reactions. During inflammatory reactions, nitric oxide and cyclooxygenase are produced by inducible nitric oxide synthase. Inflammatory factors such as prostaglandin E2, which are produced in excess by )-2, are produced. In addition, in cells induced by lipopolysaccharide (LPS), cytokines such as tumor necrosis factor-α, interleukin-6, and interleukin-1β, which are inflammatory mediators, are produced. It produces and mediates various inflammatory responses.

For the above reasons, research is being actively conducted on methods to reduce skin irritation and inflammation. Substances used as anti-inflammatory agents include non-steroidal benzydamine, indomethacin, and ibuprofen, and steroidal dexamethasone. , hydrocortisone, etc. (Leung Y.F. et al., Mol Vis. Sep 5;9:425-39 (2003), Rocksen D. et al., Clin Exp Immunol. 122(2):249-56 (2000), Endo Y. et al. ., Res Commun Chem Pathol Pharmacol. 33(2):195-206 (1981), FAVOUR C.B. et al., Med Bull. 6(1):15-8 (1958), Sato S. et al., J Osaka Dent Univ.1(1):37-49 (1967))

However, in most cases, the above substances cannot be used on the skin or the amount of use is limited, and especially in the case of steroids, they are accompanied by side effects such as skin atrophy, insomnia, and anxiety.

Therefore, the need to discover substances with antioxidant and anti-inflammatory effects from natural plants that are safe for the skin is increasing.

In particular, plants may show different effects or show differences in efficacy depending on the medicinal part used.

For example, Patent Document 1 discloses psoriasis treatment compositions such as dandelion bark, raw fat, and soybean sprouts, but there is no disclosure about the antioxidant and anti-inflammatory effects of plant root extracts, and Patent Documents 2 to 5 disclose antioxidant-related effects as plant extracts. is just starting.

Chinese Patent Publication No. 103877295 Korean Patent Registration No. 10-1127156 Korean Patent Registration No. 10-1397160 Korean Patent Registration No. 10-1768870 Korean Patent Registration No. 10-1309172

The present invention seeks to provide a composition having antioxidant and anti-inflammatory effects.

Additionally, the present invention seeks to provide a cosmetic composition containing the above composition.

The antioxidant and anti-inflammatory composition according to the present invention preferably includes one or more of extracts of peony root, burdock root, Rehmannia glutinosa root, yam root, and Aspergillus root extract.

The antioxidant and anti-inflammatory composition according to the present invention more preferably contains extracts of peony root and burdock root.

The antioxidant and anti-inflammatory composition according to the present invention more preferably contains extracts of peony root, burdock root and Rehmannia glutinosa root.

The antioxidant and anti-inflammatory composition according to the present invention more preferably contains extracts of peony root, burdock root, Rehmannia glutinosa root and yam root.

The antioxidant and anti-inflammatory composition according to the present invention more preferably contains extracts of peony root, burdock root, Rehmannia glutinosa root, yam root and Aspergillus root.

The composition is preferably in the form of any one of aerosol, liquid, solution, gel, paste, cream, ointment, foam, and powder.

It is preferable that the composition is included in any one of a pack, band, or patch.

It is more preferable that the pack is any one of topical patch, sheet type, gauze type, cream type, and gel type.

It is preferable that the composition is either a pharmaceutical composition or a cosmetic composition.

The cosmetic composition includes hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, and hair aerosol. , hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservation treatment, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, It is preferable that it is a formulation selected from the group including hair mousse and hair spray.

It is preferable that the pharmaceutical composition is a formulation selected from the group including tablets, capsules, injections, creams, gels, patches, sprays, ointments, warning agents, lotions, liniment agents, pasta agents, and cataplasma agents.

The present invention is a composition containing one or more of extracts of peony root, burdock root, Rehmannia glutinosa root, yam root, and Aspergillus root extract, and has excellent antioxidant and anti-inflammatory effects.

Figure 1 schematically shows the results of measuring the ability of the composition according to the present invention to inhibit intracellular reactive oxygen species production.
Figure 2 schematically shows the results of measuring the nitric oxide production inhibition ability of the composition according to the present invention.
Figures 3 and 4 schematically show the results of measuring inhibition of pro-inflammatory cytokine expression by the composition according to the present invention.
Figure 5 schematically shows the results of measuring the inhibition of inflammation-inducing enzyme expression by the composition according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art. Accordingly, the embodiments of the present invention may be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below.

Throughout the specification of the present invention, when a part “includes” a certain component, this means that it does not exclude other components but further includes other components, unless specifically stated to the contrary.

Throughout the specification of the present invention, when a step is said to be located “on” or “before” another step, this means not only when a step is in a direct time-series relationship with another step, but also when a step is in a direct time-series relationship with the other step, as well as a mixing step after each step. Likewise, the order of two steps can include the same rights as in the case of an indirect time-series relationship in which the time-series order can be changed.

The terms "about", "substantially", etc. used throughout the specification of the present invention are used to mean at or close to that value when manufacturing and material tolerances inherent in the stated meaning are presented, and the present invention Precise or absolute figures are used to aid understanding and to prevent unscrupulous infringers from taking unfair advantage of the disclosure. The term “step of” or “step of” used throughout the specification does not mean “step for.”

The antioxidant and anti-inflammatory composition according to the present invention is preferably a root extract rather than a plant flower, leaf, fruit, stem, etc.

The antioxidant and anti-inflammatory composition according to the present invention preferably includes one or more of extracts of peony root, burdock root, Rehmannia glutinosa root, yam root, and Aspergillus root extract.

The antioxidant and anti-inflammatory composition according to the present invention more preferably contains extracts of peony root and burdock root.

The antioxidant and anti-inflammatory composition according to the present invention more preferably contains extracts of peony root, burdock root and Rehmannia glutinosa root.

The antioxidant and anti-inflammatory composition according to the present invention more preferably contains extracts of peony root, burdock root, Rehmannia glutinosa root and yam root.

The antioxidant and anti-inflammatory composition according to the present invention more preferably contains extracts of peony root, burdock root, Rehmannia glutinosa root, yam root and Aspergillus root.

The above peony is also called Mokdan (牧丹). It is 2m tall and is grown in various places. The branches are thick and hairless. The leaves are three-layered and the small leaves are egg-shaped and split into 2 to 5 pieces. The surface of the leaf is hairless and the back side has fine hairs and is often whitish. The flowers are bisexual, bloom red in May, are over 15cm in diameter, and have a pouch-like flower stamen that surrounds the ovary. There are 5 sepals and more than 8 petals, and the size and shape are not the same, and are shaped like an upside-down egg, with deep irregular grooves on the edges. There are many stamens and 2 to 6 pistils with hair. The fruit ripens in September and the inner gland bursts to reveal seeds, which are round and black. There are many cultivated varieties, and the root bark is used for anti-inflammatory, headache, back pain, stomach upset, and hemostasis.

The burdock is 50 to 150 cm tall. The tap root grows 30 to 60 cm long, and a stem emerges from the end. The leaves attached to the roots come out in clusters and have long petioles. The stems are alternate and have a heart shape. The outside is dark green, but the back has dense white fluff, and there are teeth shaped like this on the edges. Flowers bloom from July to August and are black-purple, and the flowers hang in corymbs at the ends of branches. The involucre is round, the bracts are needle-shaped, and the ends look like hooks. The flowers are tubular, the seeds are black, and the tubular hairs are brown. The fruit is an achene and ripens in September. It is hardy, rarely suffers from diseases, is very resistant to cold, and is not particularly sensitive to soil type. Propagation is done by seed or by dividing. It is a naturalized plant native to Europe. Varieties include Nongyacheon, which has long and thick roots, and Sacheon, which has good flesh and short roots. The recipe is to make pickled vegetables or stew them and eat them as a side dish. The root contains inulin and some palmitic acid. In Europe, it is used as a diuretic and diaphoretic, and the seeds are used as a diuretic when there is swelling, and as an antidote for sore throats and poisonous insects. It is widely cultivated in Japan and grows wild in Europe, Siberia, and northeastern China.

The above-mentioned Rehmannia glutinosa is a medicinal material (Korea) made by drying the roots of Rehmannia glutinosa Liboschitz var. purpurea Makino of the Hyeonsam family. In China, dried Rehmannia glutinosa is called raw Rehmannia glutinosa. When the raw rehmannia glutinosa was soaked in water, the one that floated on water was called Cheonhwang (天黃), the half-floating and half-sinking one was called Inhwang (人黃), and the one that sank completely was called Rehmanniae (地黃). Among these, it is said that sinking in water is good, half-sinking is next, and floating is not good. It is said that the reason why it is called Rehmannia glutinosa is because it is precious to sink, so it is said to have changed from being called lower. This medicine has a peculiar smell and tastes sweet, but is a bit bitter afterward. Rehmannia glutinosa is used for fever, thirst, osteoarthritis, hypothermia, hematemesis, and nosebleeds, and treats skin rashes caused by heat. Pharmacological effects have been reported to lower blood sugar level, tonic, diuretic, protect liver function, and antibacterial activity.

The yam is also called dang yam. It mainly grows in mountainous areas. A stem emerges from a cylindrical fleshy root and wraps itself around another object. The leaves are opposite or alternate, have long petioles, are long oval or triangular, have sharp ends, have a heart base, and are hairless. Flower buds grow in the leaf axils. The flower is a solitary flower that blooms from June to July and hangs on a water inflorescence. The male flower spikes stand upright, and the female flower spikes droop downward and bear white flowers. Male flowers have six stamens, perianth segments, and one pistil, and female flowers have six perianth segments and one pistil. The fruit is a capsule with three wings, and the seeds also have membranous wings. The rhizome is edible and is also used as a tonic and anti-diarrheal agent.

The above Cheongung is native to China and is cultivated as a medicinal plant. It is 30-60cm high, is hollow, and has somewhat branched branches. The leaves are alternate, pinnately compound leaves with two or three branches, and the forked pieces are egg-shaped and have somewhat deep serrated teeth. The root leaves and lower leaves have long petioles, and the lower part becomes a leaf sheath that surrounds the stem. The flowers bloom from August to September, are white, and form a compound umbel. There are 5 petals, rolled inward, 5 stamens, and 1 pistil. There are 5 to 6 gun and rifle cannons each, shaped like a row. The fruit grows but does not mature. The internodes in the ground look like lumps 5 to 10 cm long and 3 to 5 cm in diameter and have a strong scent. It has sedative, analgesic, and tonic effects and is used to treat headaches, anemia, and gynecological diseases. From September to November, the rhizome is dug up, the leaves and stems are removed, dried in the sun, and 3 to 6 g is boiled and taken or used as a pill or powder.

The composition according to the present invention can be in the form of any one of aerosol, liquid, solution, gel, paste, cream, ointment, foam, and powder, but is not limited thereto.

For example, lotions are similar to solutions, but tend to be thicker and more emollient in nature. Lotions are usually a mixture of water and oil and often contain less alcohol than a solution.

For example, creams are an emulsion of approximately equal proportions of oil and water. It penetrates well into the outer layer of the skin's stratum corneum. Creams are thicker than lotions and retain their shape when removed from the container.

Additionally, the composition of the present invention may be included in any one of a pack, band, or patch, but is not limited thereto.

The pack may be in the form of any one of topical patch, sheet type, gauze type, cream type, and gel type.

The pack may be any one selected from a mask pack, hand pack, foot pack, calf pack, hair pack, thigh pack, abdominal pack, side pack, forearm pack, chin pack, and neck pack.

The composition of the present invention is preferably included in either a pharmaceutical composition or a cosmetic composition.

The pharmaceutical composition in the present invention is a dosage form selected from the group including tablets, capsules, injections, creams, gels, patches, sprays, ointments, warning agents, lotions, liniment agents, pasta agents, cataplasma agents, etc. It may be possible, but it is not limited to this.

The pharmaceutical composition of the present invention may further include pharmaceutically acceptable carriers, lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives, etc. commonly used in preparation.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and in case of parenteral administration, it can be administered through methods such as intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, mucosal administration, eye drop administration, and local administration. It can be administered, but is not limited to this.

The appropriate dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, ingested food, administration time, administration route, excretion rate, and reaction sensitivity. may be prescribed. Preferably, the dosage of the pharmaceutical composition of the present invention may be 0.0001-100 mg/kg (body weight) for adults, but is not limited thereto.

The selection of suitable ingredients that can be added to the present invention, as will be apparent to those skilled in the art, depends to some extent on whether the external skin composition of the present invention is formulated as a solution, lotion, cream, ointment, gel, foam, or transdermal patch. something to do.

For example, it may include colorants (pigments), flavoring agents (flavors), suspending agents, emulsifiers, solubilizers, stabilizers, isotonic agents, pH adjusters, viscosity adjusters, solvents, etc. The ingredients and contents commonly added to cosmetic compositions will be apparent to those skilled in the art.

On the other hand, the cosmetic composition of the present invention can be used as hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair Wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservation treatment, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, It may be a formulation selected from the group including hair moisturizer, hair mousse, and hair spray, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through specific examples and experimental examples of the composition according to the present invention.

[Example 1. Preparation of peony root extract]

The dried peony roots were finely ground using a grinder and powdered. After mixing peony root powder and 70% ethanol, an extraction solution was prepared for 3 hours at room temperature using a stirrer. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a peony root extract.

[Example 2. Preparation of burdock root extract]

The dried burdock roots were finely ground using a grinder and powdered. After mixing burdock root powder and 70% ethanol, an extraction solution was prepared for 3 hours at room temperature using a stirrer. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a burdock root extract.

[Example 3. Preparation of Rehmannia glutinosa root extract]

The dried Rehmannia glutinosa roots were finely ground using a grinder and powdered. After mixing Rehmannia glutinosa root powder and 70% ethanol, an extraction solution was prepared for 3 hours at room temperature using a stirrer. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a Rehmannia glutinosa root extract.

[Example 4. Preparation of yam root extract]

The dried yam roots were finely ground using a grinder and powdered. After mixing yam root powder and 70% ethanol, an extraction solution was prepared for 3 hours at room temperature using a stirrer. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a yam root extract.

[Example 5. Preparation of Cheonigung root extract]

The dried roots were finely ground using a grinder and powdered. After mixing the root powder and 70% ethanol, an extraction solution was prepared for 3 hours at room temperature using a stirrer. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a root extract.

[Example 6. Preparation of mixed extract of peony root and burdock root]

Dried peony root and burdock root powder were mixed in a ratio of 1:1, 70% ethanol was added, and an extraction solution was prepared using a stirrer at room temperature for 3 hours. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a mixed extract of peony root and burdock root.

[Example 7. Preparation of mixed extract of peony root, burdock root and Rehmannia root]

Dried peony root, burdock root, and Rehmannia glutinosa root powder were mixed in a ratio of 1:1:1, respectively, 70% ethanol was added, and an extraction solution was prepared using a stirrer at room temperature for 3 hours. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a mixed extract of peony root, burdock root, and Rehmannia glutinosa root.

[Example 8. Preparation of mixed extract of peony root, burdock root, Rehmannia root and yam root]

Dried peony root, burdock root, Rehmannia glutinosa root, and yam root powder were mixed in a ratio of 1:1:1:1, respectively, 70% ethanol was added, and an extraction solution was prepared using a stirrer at room temperature for 3 hours. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a mixed extract of peony root, burdock root, Rehmannia glutinosa root, and yam root.

[Example 9. Preparation of mixed extracts of peony root, burdock root, Rehmannia glutinosa root, yam root and Aspergillus root]

Mix dried peony root, burdock root, Rehmannia glutinosa root, yam root, and Aspergillus root powder in a ratio of 1:1:1:1:1, add 70% ethanol, and extract for 3 hours at room temperature using a stirrer. A solution was prepared. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a mixed extract of peony root, burdock root, Rehmannia glutinosa root, yam root, and Aspergillus root.

[Experimental Example 1: Measurement of inhibition ability to generate reactive oxygen species in cells]

Human-derived keratinocytes (HaCaT) were cultured on a plate for 24 hours, then treated with the control and Examples 1 to 9 and cultured for 24 hours. Each treatment group was reacted with DCFH-DA and then treated with hydrogen peroxide (H ₂ O ₂ ) to induce oxidation. By measuring the fluorescence value, the ability to inhibit the production of reactive oxygen species (ROS) occurring within cells was confirmed.

As shown in Figure 1, the reactive oxygen species inhibition ability of Examples 8 and 9 increases compared to Examples 1 to 5, and the reactive oxygen species inhibition ability of Example 9 increases compared to Example 8. Confirmed.

[Experimental Example 2: Measurement of intracellular nitric oxide production inhibition ability]

Mouse-derived macrophages (Raw264.7) were cultured on a plate for 24 hours, then treated with Examples 1 to 9 along with lipopolysaccharide (LPS), an inflammatory response-inducing substance, and cultured for 24 hours. After recovering the cell culture medium, it was reacted using Promega's Griess reagent system. By measuring the absorbance value, the ability to inhibit the production of nitric oxide (NO) occurring within cells was confirmed.

As shown in Figure 2, the ability to inhibit nitric oxide production in Examples 8 and 9 increases compared to Examples 1 to 5, and the ability to inhibit nitric oxide production in Example 9 increases compared to Example 8. Confirmed.

[Experimental Example 3: Measurement of inhibition of pro-inflammatory cytokine expression]

Human-derived keratinocytes (HaCaT) were cultured on a plate for 24 hours, then treated with Examples 1 to 9 together with lipopolysaccharide (LPS), an inflammatory response-inducing substance, and cultured for 24 hours. Cells were recovered, RNA was isolated, and RT-PCR was performed to confirm the gene expression levels of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).

As shown in Figure 3, the expression level of TNF-α in Examples 8 and 9 was lower than that in Examples 1 to 5, and the expression level of Example 9 was confirmed to be lower than that of Example 8.

In addition, as shown in Figure 4, the expression level of IL-6 in Examples 8 and 9 was lower than that in Examples 1 to 5, and the expression level of Example 9 was confirmed to be lower than that in Example 8. .

[Experimental Example 4: Measurement of inhibition of inflammation-induced enzyme expression]

Human-derived keratinocytes (HaCaT) were cultured on a plate for 24 hours, then treated with Examples 1 to 9 together with lipopolysaccharide (LPS), an inflammatory response-inducing substance, and cultured for 24 hours. The cells were recovered, RNA was isolated, and RT-PCR was performed to confirm the gene expression level of cyclooxygenase-2 (COX-2), an enzyme involved in the inflammatory response.

As shown in Figure 5, the expression level of COX-2 in Examples 8 and 9 was lower than that in Examples 1 to 5, and the expression level of Example 9 was confirmed to be lower than that in Example 8.

Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and variations are possible without departing from the technical spirit of the present invention as set forth in the claims. This will be self-evident to those with ordinary knowledge in the field.

Claims (11)

A cosmetic composition with antioxidant and anti-inflammatory effects containing ethanol extracts of peony root, burdock root, Rehmannia glutinosa root, yam root and Aspergillus root. delete delete delete delete The cosmetic composition according to claim 1, wherein the composition is in the form of any one of aerosol, liquid, solution, gel, paste, cream, ointment, foam, and powder. The cosmetic composition according to claim 1, wherein the composition is included in any one of a pack, a band, or a patch. The cosmetic composition according to claim 7, wherein the pack is one of a topical patch, sheet type, gauze type, cream type, and gel type. delete The method of claim 1, wherein the cosmetic composition is used in hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, and hair massage cream. , hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservation treatment, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dresser, hair A cosmetic composition, characterized in that it is a formulation selected from the group including lacquer, hair moisturizer, hair mousse, and hair spray. delete