KR20170133560A - A cosmetic composition for scalp and hair improvement comprising multiple extract of combined medicinal herbs - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving scalp and hair condition containing a composite medicinal herb extract composed of Rehmannia, Japanese sweet flag, Achyranthes, milkwort, Lycium root, and Cuscuta seed as an active ingredient. More specifically, the composition is capable of promoting hair growth and preventing hair loss in addition to anti-dandruff, anti-inflammation, darkening, and antioxidation effects without side effects, thereby being used as cosmetic compositions for improving scalp and hair condition and also being used for agents for external skin application.

Description

A cosmetic composition for improving scalp and hair, which is contained as an active ingredient of a complex herbal medicine extract. {A cosmetic composition for scalp and hair improvement comprising multiple extracts of combined medicinal herbs}

The present invention relates to a cosmetic composition for improving scalp and hair for improving anti-dandruff, anti-inflammation, blackening, antioxidation, hair loss prevention and hair growth promotion using a medicinal ingredient mixed ingredient including ginseng, seokchangpo, will be.

The scalp is composed of the epidermis, dermis, and subcutaneous tissue, and the outermost tissue is the epidermis. The epidermis protects the body's sensitive tissues from damage. However, due to various physical and chemical external stimuli such as external temperature, humidity change, pollutants, stress, and lack of influence, the scalp function may be lost, and furthermore, it may affect hair loss.

 In modern society, which emphasizes beauty, many people, both men and women, use hair dye, permanent hair, and the like in order to satisfy the need for expression of individuality. However, in the case of some sensitive scalp, chemical treatment such as dyeing and perming causes irritation such as itching or stinging of the scalp and inflammation, which leads to environmental hair loss.

In this way, it is possible to protect the scalp from stimulation, stress, and chemical treatment from the environment due to various causes, to promote hair growth while preventing hair loss, and to continue research on ecosystem-friendly and environmentally harmless components to humans .

There have been many attempts to improve the scalp and hair condition by applying the extract of the herbal medicine component to the scalp. However, when the extract is used (Korean Patent No. 1537773), rose flower extract, (Korean Patent No. 1509608). However, in order to prevent hair loss and to maximize the effect of hair on the hair, one kind of natural or herbal medicine alone did not have a great effect, and only hair loss prevention and wool function And thus it lacks functions such as hair conditioning and the like.

As described above, the above-mentioned cosmetic compositions have problems such as giving an economic burden and suffering to people, causing side effects, and using only temporary or fractional pharmacological mechanism, so that the effect is more excellent and continuous, There is a growing demand for compositions without side effects.

Under these circumstances, the present inventors have made extensive efforts to develop a cosmetic composition for improving scalp or hair without side effects, and as a result, it has been found that the combined herbal medicine extract of ginseng, seokchangpo, , Natural substances, skin troubles, scalp soothing effect, hair growth promotion and dandruff inhibition, etc., and the present invention has been completed.

It is an object of the present invention to provide a cosmetic composition for improving scalp or hair containing, as an active ingredient, a complex herbal medicine extract of Rhodiola, Seocheongpo, Wusel, Origin,

Another object of the present invention is to provide an external preparation for skin containing as an active ingredient a complex herbal medicine extract of Rhododendron, Seocheongpo, Woosel, Origin, Grasshopper, and Sorghum.

As one aspect for solving the above problems, the present invention provides a cosmetic composition for improving scalp or hair, which contains, as an active ingredient, a complex herbal medicine extract of Rhodiola, Seokchangpo,

As used herein, the term " Rehmannia glutinosa "is a perennial herb of Chinese origin; The dried medicinal material was dried at. It is known that it is a well-fattened jujube, and it contains ingredients such as beta-sitosterol, mannitol, stigmasterol, campesterol, and lemarin. It has the effects of hypoglycemic action, hepatoprotective action, .

The term " Acorus gramineus "as used in the present invention refers to a plant belonging to the iris family, which has an efficacy against gadam (sputum, expectorant), dry stomach, Especially in cases of diarrhea, bronchitis, and indigestion.

As used herein, the term " Achyranthes bidentata "is a perennial herbaceous plant belonging to the genus Bifidum, and may be referred to as a knot in that the shape of a node in a plant stem is similar to that of a bovine knot. The whisker is a medicinal material that corresponds to the root of the shoulder. The active ingredient contains saponin and a large amount of calcium and is known to have the effects of knee diseases (inflammation caused by arthritis, rheumatoid arthritis, bruises), hypertension, diuretic action and the like.

As used herein, the term "Polygala tenuifolia" refers to a plant belonging to the non-adherent tree. In the herb, the root portion is used for medicinal purposes and the efficacy of sedation, hypnotic action, have.

The term "Lycium chinense" as used in the present invention refers to a dried medicinal substance of the root bark of Gujuga tree, which is also referred to as a goitrous muscle, a famine, and a hungry famine, and includes blood pressure lowering action, Action, antibacterial effect and so on.

The term " Cuscuta chinensis "as used in the present invention is a seed of a Korean ginseng, which is an annual plant belonging to the family Myrosea, and is also referred to as ginseng seed. It mainly protects the liver and kidneys, clears the eyes, and has the effect of treating diabetes.

As used herein, the term "extract" refers to any conventional extraction solvent known in the art, such as (a) water, (b) C1-4 lower alcohols such as methanol, ethanol, c) a mixed solvent of the lower alcohol and water.

The extract may contain any one of the extract obtained by the extraction treatment, the diluted or concentrated liquid of the extract, the dried product obtained by drying the extract, or the adjusted product or the purified product.

The extraction can be carried out using room temperature extraction, hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction and steam extraction, but the present invention is not limited thereto.

The term "herbal medicine extract" used in the present invention may be an extract of a mixture of ginseng, seokchonpo, wooze, raw paper, coriander and soil, or may be a mixture of extracts of each component. (W / w) weight ratio of the extracts of Chinese cabbage, Chinese cabbage, Chinese cabbage, silkworm, wax, raw paper, gill pulp and soil extract to the weight ratio of 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: (W / w), more specifically 1 to 3: 1 to 3: 1 to 3: 1 to 5: 1 to 5: 1 to 5: 3: 1 to 3: 1 to 3 (w / w).

The term "blackening of hair" as used in the present invention means that the hair turns black. Like skin color, hair color is determined by the melanin produced by melanocytes. Melanogenesis, which occurs vigorously during the anagen phase of the hair growth cycle, is accomplished through the action of several enzymes that are phased in the melanosome within the melanocyte (melanocyte). It has been reported that the whitening of the hair gradually and selectively decreases the number and activity of melanocytes in the hair follicles. Thus, the extract plays an important role in the darkening of the hair by increasing the amount of melanin production.

According to one embodiment of the present invention, the total amount of melanin in the melanoma cells of the rats treated with the herbal extract of Zygosha, Seokchangpo, Wusu, (Table 1). It was confirmed that the total amount of melanin in the melanoma cells of the rats was higher than that of the melanoma cells.

As used herein, the terms "hair loss prevention" and "hair growth" refer to hair loss, hair loss, and hair loss due to various habits and environmental influences, such as genetic causes, hormone imbalance, mental stress of social life, exposure to air pollution, It means to improve the hairless condition in the area to be present, to prevent the hair loss from proceeding, and to get hairy.

According to a specific embodiment, human dermal papilla cells treated with the herbal extract of Rhizopus, Seokjangpo, Woosu, Yeonji, Gyohwabi and Sorghum of the present invention are cultured, luciferase is added to the supernatant, and the activity of luciferase is measured As a result, it was confirmed that the effect on the activation of Wnt / β-catenin signal pathway was the best in the herb extract (Table 5).

In addition, the expression of DKK-1 gene, an antagonist of Wnt / β-catenin, was amplified by RT-PCR to confirm that the herbal extracts activate Wnt / β-catenin which promotes hair growth. As a result, the treatment of the herbal medicine extract significantly decreased the expression of DKK-1, which was increased by the dihydrotestosterone treatment, and the effect was more excellent than that of the single extract. It can be seen that hair growth can be promoted through this (Table 6).

The term "dandruff generation inhibition" used in the present invention means prevention of dermatosis on the skin surface of the head caused by sebum secretion, dandruff causing bacteria, and the like. The cause of the dandruff may include, but is not limited to, a natural phenomenon, an increase in sebum secretion, a causative agent of dandruff, and a schizophrenia caused by a metabolic disorder.

According to a specific embodiment, the present invention provides a method for inhibiting growth of dandruff when compared with the control and single extracts, as compared to the control and mono extracts, as a result of an antibacterial test on the dandruff causative bacteria of the herbal extracts of Rhizophora, Seocheongpo, (Table 8).

In addition, it was confirmed that dandruff prevention effect and itching reduction effect were excellent in the shampoo containing the herbal medicine extract as compared with the conventional shampoo (Table 10).

As used herein, the term " scalp inflammation relief "means an inflammatory condition that occurs in a scalp-causing inflammatory agent in a weak and sensitive human scalp. The causative bacteria of the scalp include, but are not limited to, bacteria, fungi, fungus, and the like.

According to a specific example, RAW264.7 cell line, which is a macrophage of mice treated with a combination herbal medicine extract of Rhizophora, Seocheongpo, Woojil, Grasshopper, Grasshopper, and Sorcerer, was cultured and its absorbance was measured to determine NO- , And the combined herbal medicine extracts showed higher NO-production inhibition rates than the control and single extracts (Table 2).

In addition, the free radical scavenging ability of the herb extracts was similar to that of the control and single extracts, and the free radical scavenging ability was similar. As a natural extract, it was confirmed that the antioxidative effect was excellent without side effects (Table 3) .

The cosmetic composition for scalp or hair for improvement of the present invention contains 0.001 to 95% by weight (w / w), specifically 0.001 to 50% by weight (w / w) of the complex herbal medicine extract, (W / w), based on the total weight of the composition. However, the composition is not limited thereto, and may vary depending on the condition and progress of the patient.

The cosmetic composition for improving scalp or hair according to the present invention may contain 0.001 to 15% by weight (w / w), more preferably 0.001 to 10% by weight, of each of the above- (W / w), more specifically 0.001 to 5 wt% (w / w). However, the composition is not limited thereto, and may vary depending on the condition and progress of the patient.

The term "moisturizing agent " as used in the present invention means a moisturizing agent used by a method conventional in the art.

The cosmetic composition may further comprise a moisturizing agent, and the composite composition may be contained in an amount of 0.01 to 0.5 times the amount of the moisturizing agent added. The moisturizing agent may include 0.01 to 20% by weight, specifically 0.001 to 10% by weight, based on the total weight of the cosmetic composition. The moisturizing agent may comprise glycerin, 1,3 butylene glycol, lyroylylglutamate lysine or a non-toxic salt thereof.

The cosmetic composition of the present invention can be used not only as a complex herbal medicine extract of Zhiwu, Seokchangpo, Woosu, a raw paper, a crustacean and a soil, but also as an adjuvant commonly used in a cosmetic composition such as a hydrophilic or lipophilic gelling agent, a hydrophilic or lipophilic active agent, Solvents, perfumes, fillers, blocking agents, pigments, absorbers, dyes, and the like.

The cosmetic composition may be at least one selected from the group consisting of hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nourishing cream, hair moisturizing cream, Hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, A hair mousse or a hair spray.

Preferably, the composition of the present invention can be used by a method such as direct application or spreading on hair or scalp. The hair to which the composition of the present invention is applied includes hair follicles and hair follicles, hair and eyelashes and eyelashes, beard, armpits, pubic hair, hair follicles and hair follicles.

In another aspect, the present invention provides an external preparation for skin comprising, as an active ingredient, a complex herbal medicine extract of Rhodiola, Seokchonpo, Wusel, Origin, Grasshopper, and Sorghum.

The term "external preparation for skin " used in the present invention refers to an external preparation for skin having anti-hair loss and hair growth promoting effect containing an extract of a complex herbal medicine as an active ingredient, such as cream, gel, patch, spray, ointment, Pasta or cataplasma drugs or quasi-drugs in the form of external preparations for skin, but the present invention is not limited thereto.

The herbal composition of the present invention can be used as a cosmetic composition for improving scalp and hair by improving anti-dandruff, anti-inflammation, blackening, antioxidation, hair loss prevention and hair growth promotion without side effects In addition, there is an effect that can be usefully used as an external preparation for skin.

Figure 1 shows a primer according to an embodiment of the invention.
Fig. 2 is a test result on the effect of all of the extracts on the flow cytometry according to one embodiment of the present invention. Fig.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited by the following examples.

Example  1. Manufacture of Rice extract

Dried Korean rhubarb was purchased and used for the experiment. After thoroughly stirring, the flask was charged with 100 g of dry weight, and extracted with 1000 g of an extraction solvent (distilled water) at 70 ° C for 3 hours. The extracted hot water extracts were filtered with a filter having a pore size of 0.2 μm to prepare respective extracts.

Example  2. Seokchangpo  Preparation of extract

Dried Korean seokjangpo was purchased and used for the experiment. After finely chopping seokchonpo, 100 g of dry weight was put into a flask and extracted with 1000 g of extraction solvent (distilled water) by hot water extraction at 70 ℃ for 3 hours. The extracted hot water extracts were filtered with a filter having a pore size of 0.2 μm to prepare respective extracts.

Example  3. The  Preparation of extract

Dried domestic wheat was purchased and used in the experiment. After finishing well, the dried weight of 100 g was placed in a flask and subjected to hot extraction with 1000 g of extraction solvent (distilled water) at 70 DEG C for 3 hours. The extracted hot water extracts were filtered with a filter having a pore size of 0.2 μm to prepare respective extracts.

Example  4. Preparation of extract

Dried Korean soils were purchased and used in the experiment. After finely ground, the dried weight of 100 g was placed in a flask and subjected to hot extraction with 1000 g of extraction solvent (distilled water) at 70 ° C for 3 hours. The extracted hot water extracts were filtered with a filter having a pore size of 0.2 μm to prepare respective extracts.

Example  5. Gill pulpit  Preparation of extract

Dried Korean myofibers were purchased and used for the experiment. After finely chopping the scallops, 100 g of dry weight was placed in a flask, and extracted with 1000 g of extraction solvent (distilled water) at 70 ° C for 3 hours. The extracted hot water extracts were filtered with a filter having a pore size of 0.2 μm to prepare respective extracts.

Example  6. Manufacture of soil extract

Dried domestic soil was purchased and used for the experiment. After thoroughly finely ground, the dried weight of 100 g was placed in a flask and extracted with 1000 g of extraction solvent (distilled water) at 70 캜 for 3 hours. The extracted hot water extracts were filtered with a filter having a pore size of 0.2 μm to prepare respective extracts.

Example  7. Preparation of complex herbal medicine extract

The extracts of each of the ginseng extracts prepared in Examples 1 to 6, the extracts of Seokchangpo extract, raspberry extract, native extract, gingerolus extract and extracts of Sorghum were mixed with each other at a ratio of 1: 1 (dry weight of each extract was mixed with 1: 1) .

Experimental Example  1. The increase in total melanin Blackening  effect

Blackening effect was confirmed by measuring the total amount of melanin at the cellular level by adding the extracts of Examples 1 to 7 to the culture medium of rat B-16 mouse melanoma cells (Lotan R., Lotan D. Cancer Res 40: 3345-3350, 1980). To evaluate the toxicity of melanoma cells in rats before the experiment and to determine the concentration without toxicity, blackening evaluation was performed.

The extracts of Examples 1 to 7 were added to the culture medium to give final concentrations of 1 ppm and 10 ppm, and Forskolin (Forskolin) of 10 ppm was added to the medium to treat B-16 melanoma cells for 3 days Lt; / RTI >

Cells were then trypsinized, detached from the culture, centrifuged, and then melanin was extracted. The removed cells were incubated with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by an absorbance of 1 × 10 ⁶ cells per unit cell, and the total amount of melanin relative to the control group was calculated as an increase rate (%). The results are shown in Table 1. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Increase in the total amount of melanin at the cell level according to the concentration sample Melanin production (abs) Growth rate (%) Control group (DMSO, 10 ppm) 0.33 - The positive control (phoscholine, 10 ppm) 0.792 140 Example 1 (10 ppm) 0.499 51.21 Example 1 (1 ppm) 0.432 30.91 Example 2 (10 ppm) 0.481 45.76 Example 2 (1 ppm) 0.429 30.00 Example 3 (10 ppm) 0.506 53.33 Example 3 (1 ppm) 0.438 32.73 Example 4 (10 ppm) 0.497 50.61 Example 4 (1 ppm) 0.423 28.18 Example 5 (10 ppm) 0.512 55.15 Example 5 (1 ppm) 0.434 31.52 Example 6 (10 ppm) 0.517 56.67 Example 6 (1 ppm) 0.452 36.97 Example 7 (10 ppm) 0.546 65.45 Example 7 (1 ppm) 0.467 41.52

As a result, as shown in Table 1, the complex herbal medicine extract (Example 7) exhibited a higher total melanin amount than that of the control or single extract treatment group (Examples 1 to 6) to rat melanoma cells It was confirmed that it can be used for blackening purposes.

Experimental Example  2. Anti-inflammatory effects of inhibition of NO production

In order to confirm the anti-inflammatory effect and the skin trouble relieving effect of the extracts of Examples 1 to 7, nitrite oxide (NO) formation inhibition experiment was carried out by the GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates to enter 3x105 cells into each well, and cultured for 24 hours. Subsequently, the extracts of Examples 1 to 7 were replaced with a diluted cell culture medium at a final concentration of 1, 10, 100 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated together as a positive control and cultured for 30 minutes. LPS (Lipopolysaccharide) . 100 μl of the supernatant was transferred to a 96-well plate, and 100 μl of the GRIESS solution was added thereto at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect of the extracts of Examples 1 to 7, The results are shown in Table 2 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Anti-inflammatory effect - NO generation inhibitory effect sample NO production inhibition rate (%) Control group (DMSO, 10 ppm) - L-NMMA (positive control, 10 ppm) 41.02 Example 1 (10 ppm) 32.15 Example 2 34.81 Example 3 31.94 Example 4 35.27 Example 5 29.62 Example 6 37.29 Example 7 47.69

As a result, as shown in Table 2, the complex herbal medicine extract has high activity and exhibits excellent activity as a natural substance when compared with L-NMMA, which is a representative anti-inflammatory drug, and the sole extract, Anti-inflammatory effect acts as an auxiliary role, and synergy effect can be expected.

Experimental Example  3. Free radical Scatters  Antioxidative effect by confirmation

The free radical scavenging ability of the herbal extracts was measured by the 1,1-diphenyl-2-picryl hydrazine (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical, which shows maximum absorption at 517 nm in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma, and dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of the herbal extract or the positive control vitamin C was added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).

The concentration of the extract when the absorbance of the sample was half of the absorbance of the control group was expressed by IC50, and the results are shown in Table 3 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

sample IC50 (% w / v) Control group 0.0025 Positive control (vitamin C) concentration 0.0005 Example 1 Concentration 0.0011 Example 2 Concentration 0.0019 Example 3 Concentration 0.0022 Example 4 Concentration 0.0016 Example 5 Concentration 0.0015 Example 6 Concentration 0.0009 Example 7 Concentration 0.0006

As a result, as shown in Table 3, the complex herbal medicine extract had similar free radical scavenging ability compared with vitamin C and single extract, and it was confirmed that the antioxidative effect was excellent without side effects when considering that it is a natural extract.

Experimental Example  4. Assessment of cytotoxicity

In this experiment, cell division of Follicle Dermal Papilla (Promocell, Germany) was measured by Cell Counting Kit-8 (Dojindo Lab, USA) for the evaluation of cytotoxicity of the herbal extracts.

First, a uniform number of cells were plated on a 96-well plate and stabilized, and then the following extracts were treated to 10, 20 ug / ml and then further cultured for 48 hours. Thereafter, CCK-8 was treated and the absorbance was measured at 450 nm for 2 hours. The relative cell viability of the cells treated with the untreated control extract was analyzed as follows, and the results are shown in Table 4 below. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

Relative cell viability = (compound treated group) / (untreated group) X 100

sample Survival rate (%) No treatment 100 BIO 1uM 103 Example 1 (10 ug / ml) 93 Example 1 (20 ug / ml) 102 Example 2 (10 ug / ml) 94 Example 2 (20 ug / ml) 102 Example 3 (10 ug / ml) 101 Example 3 (20 ug / ml) 104 Example 4 (10 ug / ml) 94 Example 4 (20 ug / ml) 102 Example 5 (10 ug / ml) 101 Example 5 (20 ug / ml) 105 Example 6 (10 ug / ml) 97 Example 6 (20 ug / ml) 107 Example 7 (10 ug / ml) 92 Example 7 (20 ug / ml) 103

As a result, as shown in Table 4, the number of cells was not greatly changed by the sample treatment of each extract, and it was confirmed that there was no cytotoxicity.

Experimental Example  5. Extract of Wnt / β- catenin  signal pathway active effect

Activation of the Wnt / β-catenin signal pathway promotes hair growth, so we investigated the effect of Wnt / β-catenin signal pathway activation on extracts to promote hair growth using TOPflash reporter (Millipore, USA). Human dermal papilla cells were inoculated in a 24-well plate at 2 x 10 < ⁵ > / well and cultured for 24 hours. Then, the extracts were treated to 10, 20 ug / ml and then further cultured for 15 hours, followed by dual luciferase assay kit (Promega, USA) was used to measure the firefly luciferase activity. All results were expressed as mean ± standard deviation (SD) after three or more independent experiments.

In order to accurately measure the activity of luciferase, each sample was run 3 times, and the relative activity to the control group was calculated as an increase rate (%) and is shown in Table 5 below. The control group is untreated group. The positive control was 6-bromoindirubin-3'-oxime (BIO) and is known to inhibit GSK-3 in the Wnt signaling pathway.

sample Growth rate (%) Control group (no treatment) 100 BIO 1uM 152 Example 1 (10 ug / ml) 137 Example 1 (20 ug / ml) 178 Example 2 (10 ug / ml) 146 Example 2 (20 ug / ml) 192 Example 3 (10 ug / ml) 134 Example 3 (20 ug / ml) 179 Example 4 (10 ug / ml) 143 Example 4 (20 ug / ml) 182 Example 5 (10 ug / ml) 127 Example 5 (20 ug / ml) 164 Example 6 (10 ug / ml) 134 Example 6 (20 ug / ml) 175 Example 7 (10 ug / ml) 162 Example 7 (20 ug / ml) 203

As a result, as shown in Table 5, each of the extracts of the present invention increased the TOPflash reporter activity in a concentration-dependent manner, showing selective activity against Wnt / β-catenin signaling, Example 7). The measured values corrected the firefly luciferase level to the renilla luciferase level.

Experimental Example  6. On DHT  Induced by DKK -1 inhibitory effect

Activation of Wnt / β-catenin promotes hair growth while male hormone or dihydrotestosterone (WAKO, Japan) acts as an antagonist of Wnt / β-catenin through the promotion of DKK-1 gene expression.

Therefore, in order to investigate whether the herbal extracts inhibit the growth of DKK-1 gene, which is an antagonist of Wnt / β-catenin, human dermal papilla cells were treated with 20 ug / ml of extract and 100 nM of dihydrotestosterone for 24 hours After that, the expression of DKK-1 was confirmed by RT-PCR using the primer (Bioneer, Korea) shown in FIG. The results were analyzed by using a comparative CT (ΔΔCT) Experiment (Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-Delta Delta C (T) (2001)), and internal control was performed using GAPDH, wherein the primers used were shown in Figure 1. The results are also shown in Table 6.

sample DKK-1 relative expression value Control group 0.32 DHT 100 nM 1.00 BIO 1uM + DHT 100nM 0.70 Example 1 20 ug / ml + DHT 100 nM 0.74 Example 2 20 ug / ml + DHT 100 nM 0.71 Example 3 20 ug / ml + DHT 100 nM 0.72 Example 4 20 ug / ml + DHT 100 nM 0.68 Example 5 20 ug / ml + DHT 100 nM 0.74 Example 6 20 ug / ml + DHT 100 nM 0.72 Example 7 20 ug / ml + DHT 100 nM 0.62

As a result, as shown in Table 6, the treatment with the herbal extract significantly decreased the expression of DKK-1 by dihydrotestosterone treatment, and the effect was more excellent than that of the single extract. It can be seen that hair growth can be promoted through this.

Experimental Example  7. Dermal papilla cells  Proliferation effect

On day 0, cells were divided into 5,000 cells / well at 10:00 am. On Day 1, the depleted medium was replaced at 10 am and cultured for 24 hours. On day 2, the measurement of CCK-8 (initial value average: 0.586) and sample treatment before sample treatment were performed at 10:00 am. At this time, 100ppm of the herbal extracts and 100ppm / 100ppm of the remaining 6 extracts were treated. On Day 5, CCK-8 was measured at 10 am. The control value averaged 0.556, indicating that the cells did not die and maintained the initial value.

The culture media used were 1) DMEM (10% FBS, 1% P / S), 2) depletion media: 5% charcoal stripped FBS containing phenol-red free DMEM media, % P / S) was used. The results obtained when the sample treatment was treated at 100 ppm are shown in Table 7 and FIG.

Average (ratio) Stdev Control (DMEM) 1.00 0.03 SF (serum free) 0.68 0.04 Example 1 (Reversible) 1.15 0.07 Example 2 (Seok Changpo) 0.84 0.05 Example 3 (wax) 1.13 0.06 Example 4 (Paper) 1.15 0.12 Example 5 (GFP) 1.22 0.1 Example 6 (Tozan) 0.77 0.03 Example 7 (herbal medicine extract) 1.32 0.07

As a result, as shown in Table 7 and FIG. 2, it was confirmed that the pelvis cells were more proliferated in the herbal extract than in the single extract. It can be seen that this can promote hair growth.

Experimental Example  8. Antidote  effect

Antimicrobial effect of Pityrosporum ovale, a causative agent of dandruff, was tested. As a test sample, Magnolia was compared and evaluated as a control group in addition to the extract.

The minimum inhibitory concentration (MIC) assay was used for the experiment. Three strains of common bacteria, two strains of fungi, and pityrosporum ovalet were used as test strains. Bacterial-MHB2 and fungus-PDB medium were applied and serial dilution method was performed, and 10 ⁶ levels of bacteria were inoculated into each well. Three kinds of general bacteria, two kinds of fungi, and Pityrosporum ovalet were inoculated and cultured, and then the degree of growth of the cells was observed for each sample concentration to determine the minimum concentration for inhibiting the germ growth. The results are shown in Table 8.

Minimum inhibitory concentration (%) E. coli
Escherichia coli S. aureus
Fungus P. aeruginosa
P. aeruginosa A. niger black mold C. albicans yeast Pityrosporum ovale
Dandruff Zinc pyrichion 0.002 0.001 0.002 0.02 0.02 0.001 Example 1 One One One 2 One One Example 2 0.01 0.01 0.01 0.5 0.5 0.01 Example 3 One 0.1 One 2  One One Example 4 One 0.1 One One One One Example 5 0.01 0.01 0.01 0.2 0.2 0.01 Example 6 0.01 0.01 0.01 0.1 0.1 0.01 Example 7 0.01 0.01 0.01 0.02 0.02 0.01

As a result, as shown in Table 8, in the MIC measurement, the effect of the complex herbal extract on the growth inhibition of dandruff growth was similar to that of the positive control group, zinc pyrithione, and the efficacy was superior to that of the single extract.

Experimental Example  9. Dandruff prevention and itching reduction evaluation

A shampoo was prepared by a conventional method according to the prescription of Table 9 below

Compounding ingredient Execution Formulation Example 1 Comparative Formulation Example 1 Polyquaternium 0.5 0.5 Sodium lauryl sulfate (30%) 20 20 Sodium polyoxyethylene lauryl sulfate 30 30 Coconut oil fatty acid diethanolamide 3 3 Quaternary ammonium cationic surfactant 2 2 Carbopol-1342 0.3 0.3 Paraoxybenzoic acid ester 0.2 0.2 Example 7 0.5 - Spices and Pigments Suitable amount Suitable amount Purified water Balance (100 total) Balance (100 total)

Ten men and women with a relatively large amount of dandruff were selected and shampoos of Examples and Comparative Examples were used for one month, respectively. Before the start of the test, the dandruff was sampled with a normal shampoo, and the dandruff accumulated for 3 days was sampled. The sampled dandruff was compared with the dandruff accumulated for 3 days after the test with the shampoo of each of the examples and the comparative example Respectively.

At this time, the collected dandruff was obtained by allowing the skilled tester to wash the scalp using about 5 g of the sample, then rinse with about 2 to 3 liters of water, and then rinsed with 800 micro-pore size filter The dry weight of the dandruff of the filtered state was measured, and the dandruff reduction rate was determined by the following equation.

After randomly selecting 10 men and women who feel a lot of itchiness and using the shampoo of Examples and Comparative Examples for 8 weeks for every 2 days for each product, the dandruff prevention effect and the reduction effect of itching were evaluated by the interview standard . The criteria for the anti-dandruff effect are as follows:

5 points - Dandruff prevention effect is very good,

4 points - Good anti-dandruff effect,

3 points - Dandruff prevention effect is normal,

2 points - little dandruff effect,

1 point - No dandruff effect at all.

The criteria for the evaluation of the itching reduction effect are as follows:

5 points - Very good itching effect,

4 points - good itching,

3 points - The itching effect is normal,

2 points - slight itching effect,

1 point - No itching effect.

The results according to the evaluation criteria are shown in Table 10.

Execution Formulation Example 1 Comparative Formulation Example 1 Dandruff reduction rate (%) 34.8 19.7 Anti-dandruff effect 3.7 2.4 Itching reduction effect 3.6 2.5

As a result, as shown in Table 10, referring to the results of dandruff reduction rate measurement, the shampoo containing the herbal extract of the present invention showed superior dandruff reduction rate than the conventional shampoo.

Also, referring to the evaluation results of dandruff prevention effect and itching reduction effect, it was confirmed that the dandruff prevention effect and the itching reduction effect are superior to the conventional shampoo when using the shampoo containing the herbal medicine extract according to the present invention.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

<110> LG HOUSEHOLD & HEALTH CARE LTD. <120> A cosmetic composition for scalp and hair care          multiple extracts of combined medicinal herbs <130> KPA160345-KR <160> 2 <170> KoPatentin 3.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> sd primer forward <400> 1 tgatgagtac tgcgctagtc 20 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> sd primer reverse <400> 2 ctcctatgct tggtacacac 20

Claims (8)

A cosmetic composition for improving the scalp or hair containing an extract of a complex herb medicine comprising ginseng, seokchonpo, wuss, raw paper, gigoba and soil as active ingredients.
The method according to claim 1,
Wherein the composition is characterized by having at least one of the following effects.
a) blackening of hair;
b) preventing hair loss or promoting hair growth;
c) suppression of dandruff formation; And
d) Scalp relief
The method according to claim 1,
Wherein the complex herbal extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
The method according to claim 1,
Wherein the weight ratio of the herbal extract to the herbal extract is 1:10 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10:10 (w / w) RTI ID = 0.0 &gt; 1, &lt; / RTI &gt;
The method according to claim 1,
Wherein the complex herbal extract comprises 0.001 to 95% by weight, based on the total weight of the cosmetic composition.
The method according to claim 1,
Wherein the cosmetic composition further comprises a moisturizing agent.
The method according to claim 1,
The cosmetic composition may be at least one selected from the group consisting of hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nourishing cream, hair moisturizing cream, Hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair shampoo, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, Hair mousse or hair spray.
A skin external preparation for improving scalp or hair containing an active ingredient of a herbal extract of Ganghwag, Seokchangpo, Wusu, Jejudo, Gigobee, and Tozan.

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