KR20180094820A - Composition for prevention of losing hair or promotion of growing hair comprising bean extract - Google Patents
Composition for prevention of losing hair or promotion of growing hair comprising bean extract Download PDFInfo
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- KR20180094820A KR20180094820A KR1020180094374A KR20180094374A KR20180094820A KR 20180094820 A KR20180094820 A KR 20180094820A KR 1020180094374 A KR1020180094374 A KR 1020180094374A KR 20180094374 A KR20180094374 A KR 20180094374A KR 20180094820 A KR20180094820 A KR 20180094820A
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- hair
- extract
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- ethanol
- extracted
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Images
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
Abstract
Description
본 발명은 콩 추출물을 포함하는 탈모 방지 또는 육모 촉진용 조성물에 관한 것이다.The present invention relates to a composition for preventing hair loss or promoting hair growth comprising a soybean extract.
대부분의 동물들은 계절에 따라 털이 한꺼번에 빠지고 새로 털이 나는 털갈이를 하나, 사람은 전체 약 10만개 정도의 모발 중 매일 100개 정도가 빠지고, 또한 매일 약 100개 정도가 새로 태어나면서 항상 비슷한 개수를 유지한다.Most animals fall into hairs all at once and new hairy molasses, with about 100 of them hairs per day of about 100,000 hairs in total, and about 100 hairs per day are kept fresh each year .
모발의 주기(hair cycle)는 성장기, 퇴행기, 휴지기의 3주기로 크게 나눌 수 있다. 그 중 성장기(anagen)는 모유두에서 활발한 세포 분열이 일어나 새로운 모발의 성장이 촉진되는 시기로, 이 시기에만 모발이 성장한다. 각 모발은 대략 남성은 3~5년, 여성은 4~6년 정도의 성장기를 가지므로 전체적으로 볼 때 약 80~85%의 모발이 성장기에 해당한다. 퇴행기(catagen)는 세포 분열이 점차 멈추어 가는 시기로 대략 3~4주 정도이다. 마지막으로 휴지기(talogen)는 모유두가 위축되어 모발이 모세혈관과 분리되어 단순히 두피에 박혀 있는 시기이다. 대략 3개월 정도이며 휴지기에 있는 모발은 대개 물리적 자극에 의해 쉽게 빠진다.The hair cycle can be roughly classified into three periods: growth period, regressor period, and rest period. Among them, anagen is a period when active division of cells occurs in the dermal papilla and the growth of new hair is promoted. During this period, the hair grows. Each hair has a growth period of approximately 3 to 5 years for males and 4 to 6 years for females, and as a whole, approximately 80 to 85% of hair corresponds to growth. The catagen is about 3 to 4 weeks when cell division is gradually stopped. Finally, the taloge is a period when the hair is separated from the capillaries and is simply put on the scalp. Approximately three months, and the hair in the rest period is usually easily removed by physical stimuli.
본 발명은 탈모 방지 또는 육모 촉진용 및 모발 건강 증진용 조성물을 제공하고자 한다.The present invention provides a composition for preventing hair loss or for promoting hair growth and for improving hair health.
본 발명의 일측면은 1% 내지 70%(v/v) 농도의 C1~C5 알코올로 추출한 콩 추출물을 유효 성분으로 포함하는 탈모 방지 또는 육모 촉진용 조성물을 제공한다.One aspect of the present invention provides a composition for promoting hair loss prevention or hair growth comprising an extract of soybeans extracted with a C 1 to C 5 alcohol having a concentration of 1 to 70% (v / v) as an active ingredient.
본 발명의 다른 일측면은 1% 내지 70%(v/v) 농도의 C1~C5 알코올로 추출한 콩 추출물을 유효 성분으로 포함하는 모발 건강 증진용 조성물을 제공한다.Another aspect of the present invention provides a composition for promoting hair health comprising a soybean extract extracted with a C 1 to C 5 alcohol having a concentration of 1% to 70% (v / v) as an active ingredient.
본 발명의 또 다른 일측면은 상기 조성물을 포함하는 화장품, 식품 또는 약학 조성물을 제공한다.Another aspect of the present invention provides a cosmetic, food or pharmaceutical composition comprising the composition.
본 발명에 따른 조성물은 저급 알코올, 구체적으로 저농도의 저급 알코올로 추출한 콩 추출물을 유효 성분으로 포함함으로써, 모세 혈관의 혈액 순환을 개선하여 모근의 상피 세포에 영양을 공급하고, 모근을 자극하여 모유두 세포의 활성화를 유도한다. 따라서 탈모의 진행을 억제함과 동시에 육모를 촉진하고, 나아가 모발과 두피의 노화를 방지하고 모발 건강을 증진시키는 효과가 우수하다. 또한 각 모발에 있어, 휴지기에서 성장기로의 이행을 촉진함으로써 육모를 촉진하는 효과가 뛰어나다.The composition according to the present invention comprises a soybean extract extracted with a lower alcohol, specifically a lower alcohol at a low concentration, as an active ingredient, thereby improving the blood circulation of the capillary blood vessels, supplying nutrition to the epithelial cells of the hair follicle, stimulating the hair follicle, Lt; / RTI > Therefore, it suppresses the progress of hair loss, promotes hair growth, further prevents aging of hair and scalp, and improves hair health. In addition, in each hair, the effect of promoting hair growth is promoted by promoting the transition from the resting period to the growing period.
도 1은 콩 추출물 적용에 따른 마우스의 모발 성장 정도를 보여주는 사진이다.FIG. 1 is a photograph showing the degree of hair growth in a mouse according to application of a soybean extract. FIG.
본 명세서에서 "탈모"는 두피로부터 모발이 탈락하는 현상 또는 모발이 성기거나 가늘어지는 상태를 의미하며, "탈모 방지"라 함은 상술한 바와 같은 탈모를 예방하고 억제하는 것을 의미하고, "육모 촉진"이라 함은 새로운 모발의 생성 촉진뿐만 아니라 기존 모발이 건강하게 자라도록 하는 것을 의미한다.As used herein, the term " hair loss "means prevention of hair loss and prevention of hair loss as described above, and" hair growth promotion "Refers to promoting the creation of new hair as well as allowing existing hair to grow healthily.
모발의 성장은 온도 또는 일광과 같은 환경적인 요인, 개체의 영양 상태와 질환 유무, 호르몬, 분만 여부, 방사선 또는 각종 약물에의 노출 여부 등 여러 가지 요인에 영향을 받는다. 탈모의 주 요인은 크게 생리적인 측면의 신체 내적인 요인과 외부 환경에 의한 외적인 요인으로 나눌 수 있다. 생리적인 측면의 신체 내적인 요인으로는 남성호르몬(androgen)의 일종으로 알려져 있는 테스토스테론(testosterone)과 테스토스테론을 DHT(dihydrotestosterone)로 변환 시켜 피지의 분비를 증가시키는 5-알파 환원효소(5-alpha reductase)의 활성 증가를 예로 들 수 있다. 외부 환경에 의한 외적인 요인으로는 혈액 순환이 원활하게 이루어지지 않아 발생하는 혈관 위축에 따른 모근에로의 영양 공급 부족, 두피 건조 등을 들 수 있다. 이를 토대로 탈모를 방지하고 육모를 촉진하기 위해서, 혈관을 확장시켜 모근에 영양 공급이 잘 이루어지도록 하거나, 모발의 구성 성분과 동일한 작용을 하는 성분을 직접 모근에 공급하거나, 세정제 또는 피지 분비 억제제를 이용하여 모근 부위의 과잉 피지를 제거하거나, 보습제를 이용하여 두피의 건조를 막는 방법 등이 이용될 수 있다.Hair growth is influenced by various factors such as temperature or sunlight, the nutritional status of the individual, the presence or absence of the disease, the presence of hormones, delivery, and exposure to radiation or various drugs. The main factors of hair loss are physiological internal factors and external factors. Physiological aspects of the body include testosterone, a type of male hormone (androgen), and 5-alpha reductase, which converts testosterone into DHT (dihydrotestosterone) to increase secretion of sebum For example). Outside factors due to the external environment include lack of nutritional supply to the hair follicles due to vascular atrophy caused by a lack of blood circulation, and scalp drying. On the basis of this, in order to prevent hair loss and promote hair growth, it is necessary to expand the blood vessels so that the nutrients are supplied to the hair follicles, or to supply directly the hair follicles with the components having the same action as the hair components, or by using a detergent or an anti- To remove excess sebum in the hair follicles, or to prevent drying of the scalp using a moisturizing agent.
검은콩을 예로 들 수 있는 콩에 포함되어 있는 비타민 E, 불포화 지방산 또는 안토시아닌은 혈관을 확장시킬 수 있으므로 혈액 순환이 원활하게 이루어지도록 할 수 있다고 알려져 있다. 지금까지 콩에 관한 연구는 약리 활성을 나타내는 유효 성분을 분리 및 정제하는 방법의 측면에서 주로 이루어졌으며, 콩 자체의 의약적 용도에 대한 연구는 거의 이루어지지 않았다. 또한 그 추출 방법에 있어서도, 천연물에 통상적으로 이용하는 추출 방법인, 고농도의 유기 용매를 이용하는 추출 방법을 주로 사용해 왔다. 콩 추출물에는 밝혀진 성분 외에 여러 미지의 성분들이 있으며, 이들 중 일부는 인체에 유용한 약리 효과를 나타낼 수 있다. 또한 그 유용한 성분은 통상의 고농도 유기 용매를 이용한 추출 방법에 의해서는 추출되지 않을 수 있다. 본 발명자들은 천연물 추출에서 일반적으로 사용되는 추출법에 대한 인식에서 벗어나, 저농도의 유기 용매를 추출 용매로 사용하여 콩으로부터 추출물을 획득하였고, 이 추출물이 고농도 유기 용매로 추출한 추출물보다 더 강력한 탈모 방지 또는 육모 촉진 및 모발 건강 증진 효과를 가짐을 밝혀냈다.It is known that vitamin E, unsaturated fatty acids or anthocyanins contained in soybeans, such as black soybeans, can expand the blood vessels and thus smooth blood circulation. So far, studies on soybean have mainly been conducted in terms of methods for separating and purifying an active ingredient exhibiting pharmacological activity, and research on the medicinal use of soybean itself has hardly been conducted. Also, in the extraction method, an extraction method using an organic solvent having a high concentration, which is an extraction method usually used for natural products, has been mainly used. There are many unknown ingredients in the soybean extract, in addition to the known ingredients, some of which may have pharmacological effects useful in the human body. In addition, the useful components may not be extracted by a conventional extraction method using a high concentration organic solvent. The inventors of the present invention have found that extracts obtained from soybeans by using a low concentration of organic solvent as an extraction solvent are devoid of recognition of the extraction method commonly used in extracting natural products and that the extracts are more effective than the extracts extracted with high concentration organic solvents, Promoting and hair health promoting effect.
이에 본 발명의 일측면은 1% 내지 70%(v/v) 농도의 C1~C5 알코올로 추출한 콩 추출물을 유효 성분으로 포함하는 모발용 조성물, 구체적으로 탈모 방지 또는 육모 촉진용 조성물 및 모발 건강 증진용 조성물을 제공한다. 상기 콩 추출물은 모유두 세포의 활성화를 유도하여 증식을 촉진하는 효과 및 모세 혈관을 확장시켜 혈액 순환을 활성화하여 모근에 영양을 공급하는 효과가 있다. 또한 휴지기의 모발에 대해 성장기로의 이행을 촉진한다. 따라서 이를 유효 성분으로 포함하는 본 발명에 따른 조성물은 탈모를 방지하고, 육모를 촉진하며, 나아가 모발의 노화를 방지하고 모발의 윤기를 높이며, 모근을 강화하여 모발 건강을 증진시킬 수 있다.Accordingly, one aspect of the present invention is to provide a composition for hair containing, as an active ingredient, a soybean extract extracted with a C 1 to C 5 alcohol having a concentration of 1 to 70% (v / v), specifically a composition for promoting hair loss prevention or hair growth, Thereby providing a composition for promotion. The soybean extract has the effect of promoting the activation of the dermal papilla cells and promoting the proliferation and expanding the capillary blood vessels, thereby activating the blood circulation and supplying nutrition to the hair follicle. It also promotes the transition to growth period for the hair of the resting period. Therefore, the composition according to the present invention containing the active ingredient as an active ingredient can prevent hair loss, accelerate hair growth, prevent aging of hair, increase hair shine, and enhance hair health by strengthening hair root.
본 발명의 일측면에서, 상기 조성물은, 저농도의 유기 용매를 이용하여 추출한 콩 추출물을 유효 성분으로 포함하는 것을 특징으로 한다. 본 발명의 다른 일측면에서, 상기 유기 용매는 C1~C5 알코올을 포함한다. 상기 C1~C5 알코올은, 예를 들어, 메탄올, 에탄올, 이소프로필알코올, n-프로필알코올, n-부탄올 및 이소부탄올로 구성된 군으로부터 선택된 어느 하나 또는 둘 이상의 혼합 용매일 수 있으며, 구체적으로 에탄올일 수 있으나, 이에 제한되는 것은 아니다. 본 발명의 또 다른 일측면에서, 상기 C1~C5 알코올의 농도는 1 내지 70%(v/v), 구체적으로 1 내지 40%(v/v), 더 구체적으로는 5 내지 30%(v/v), 보다 더 구체적으로는 12 내지 25%(v/v)일 수 있다.In one aspect of the present invention, the composition is characterized by comprising a soybean extract extracted using a low-concentration organic solvent as an active ingredient. In another aspect of the present invention, the organic solvent comprises a C 1 to C 5 alcohol. The C 1 to C 5 alcohols may be any one or a mixture of two or more selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol, Ethanol, but is not limited thereto. In another aspect of the present invention, the concentration of the C 1 to C 5 alcohol is 1 to 70% (v / v), specifically 1 to 40% (v / v), more specifically 5 to 30% v / v), and even more specifically 12 to 25% (v / v).
본 발명의 일측면에서, 콩은 그 추출물이 탈모 방지, 육모 촉진 또는 모발 건강 증진 효과를 가지는 콩을 모두 포함한다. 본 발명의 다른 일측면에서, 콩은 검은콩 또는 유색콩을 포함한다. 본 발명의 또 다른 일측면에서, 콩은 서리태, 서목태, 흑태, 황태, 푸르대콩(청태), 강낭콩, 얼룩강낭콩, 울타리콩, 녹두, 적두, 거두, 콩나물콩, 선비콩 및 대두로 구성된 군으로부터 선택된 하나 이상을 포함한다.In one aspect of the present invention, the soybean contains all of the soybean extracts having the effect of preventing hair loss, accelerating hair growth, or improving hair health. In another aspect of the invention, the beans comprise black beans or colored beans. In another aspect of the present invention, the soybean is selected from the group consisting of Seolite, Seomyeutae, Blackcurrant, Chrysanthemum, Fuda bean, Kidney bean, Stark bean, Fence bean, Mung bean, Red bean, And includes at least one selected.
본 명세서에서 "검은콩(black bean)"이란, 낟알 껍질의 색이 검은 빛깔을 띠는 콩을 총칭한다. 상기 검은콩의 예로는, 서리태, 서목태 및 흑태 등을 들 수 있으나, 이에 제한되는 것은 아니다. 검은콩의 명칭은 지역이나 분류, 방언의 영향 등에 의해 다양하게 불릴 수 있다. 본 명세서에서 "검은콩 추출물"이란, 다양한 추출 방법을 통해 검은콩에서 추출된 물질을 총칭하며, 예를 들어, 유기 용매 등을 이용하여 추출된 물질, 추출된 물질에 대한 다양한 분획물 등을 포함한다. As used herein, the term "black bean" refers to soybeans having a black color in the color of the grain shell. Examples of the black beans include, but are not limited to, seaweed, seomyeotae, and black soybeans. The name of black bean can be variously called by region, classification, influence of dialect. As used herein, the term "black bean extract" refers to a substance extracted from black beans through various extraction methods, and includes, for example, a substance extracted using an organic solvent or the like, various fractions of the extracted substance, and the like .
본 명세서에서 "유색콩(colored-bean)"이란, 낟알 껍질의 색이 짙은 빛깔을 띠는 콩을 총칭하며, 검은색 뿐만 아니라 적색, 황색 또는 청색 등의 빛깔을 띠는 콩을 포괄한다. 상기 유색콩의 예로는, 서리태, 서목태, 흑태, 황태, 푸르대콩, 강낭콩, 얼룩강낭콩, 울타리콩, 녹두, 적두, 거두, 콩나물콩, 선비콩 또는 대두 등을 들 수 있으나, 이에 제한되는 것은 아니다. 유색콩의 명칭은 지역이나 분류, 방언의 영향 등에 의해 다양하게 불릴 수 있다. 본 명세서에서 "유색콩 추출물"이란, 다양한 추출 방법을 통해 유색콩에서 추출된 물질을 총칭하며, 예를 들어, 유기 용매 등을 이용하여 추출된 물질, 추출된 물질에 대한 다양한 분획물 등을 포함한다.As used herein, the term "colored-bean" refers to soybeans having a dense color of grain shells, and includes not only black but also beans having a color such as red, yellow or blue. Examples of the above-mentioned colored beans include, but are not limited to, Seoritae, Seomotei, Black tea, Yellowtail, Fuda bean, Kidney bean, Stain kidney bean, Fence bean, Mung bean, Red bean, Reed, Soybean bean, Soybean bean or Soybean . The names of colored beans can be variously called by region, classification, and the influence of dialects. In the present specification, the term "colored bean extract" refers to a substance extracted from colored beans through various extraction methods, and includes, for example, a substance extracted using an organic solvent or the like, various fractions of the extracted substance, and the like .
본 발명의 일측면에 따른 조성물은 조성물 전체 중량을 기초로 0.001 내지 90 중량%, 구체적으로 1.0 내지 70 중량%, 더 구체적으로 5.0 내지 50 중량%의 콩 추출물을 포함한다. 상기 중량%로 포함되는 경우 본 발명의 의도한 효과를 나타내기에 적절할 뿐만 아니라, 조성물의 안정성 및 안전성을 모두 만족할 수 있으며, 비용 대비 효과의 측면에서도 상기 범위로 포함되는 것이 적절할 수 있다.The composition according to one aspect of the present invention comprises from 0.001 to 90% by weight, specifically from 1.0 to 70% by weight, more particularly from 5.0 to 50% by weight, of soybean extract, based on the total weight of the composition. When it is contained in the above weight percentage, it is not only suitable for exhibiting the intended effect of the present invention but also can satisfy both the stability and safety of the composition, and may be suitably included in the above range in terms of cost effectiveness.
본 발명의 일측면은 1% 내지 70%(v/v) 농도의 C1~C5 알코올로 추출한 콩 추출물을 포함하는 조성물을 포함하는 화장품 조성물을 제공한다.One aspect of the present invention provides a cosmetic composition comprising a composition comprising a soybean extract extracted with a C 1 to C 5 alcohol at a concentration of 1% to 70% (v / v).
상기 화장품 조성물은 국소 적용에 적합한 모든 제형으로 제공될 수 있다. 예를 들면, 용액, 수상에 유상을 분산시켜 얻은 에멀젼, 유상에 수상을 분산시켜 얻은 에멀젼, 현탁액, 고체, 겔, 분말, 페이스트, 포말(foam) 또는 에어로졸 조성물의 제형으로 제공될 수 있다. 이러한 제형의 조성물은 당해 분야의 통상적인 방법에 따라 제조될 수 있다.The cosmetic composition may be provided in any formulation suitable for topical application. For example, it may be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.
상기 화장품 조성물은 상기한 물질 이외에 주 효과를 손상시키지 않는 범위 내에서, 바람직하게는 주 효과에 상승 효과를 줄 수 있는 다른 성분들을 함유할 수 있다. 본 발명에 따른 화장품 조성물은 비타민, 고분자 펩티드, 고분자 다당 및 스핑고 지질로 이루어진 군에서 선택된 물질을 포함할 수 있다. 또한 본 발명에 따른 화장품 조성물은 보습제, 에몰리언트제, 계면 활성제, 자외선 흡수제, 방부제, 살균제, 산화 방지제, pH 조정제, 유기 및 무기 안료, 향료, 냉감제 또는 제한(制汗)제를 포함할 수 있다. 상기 성분의 배합량은 본 발명의 목적 및 효과를 손상시키지 않는 범위 내에서 당업자가 용이하게 선정 가능하며, 그 배합량은 조성물 전체 중량을 기준으로 0.01~5 중량%, 구체적으로 0.01~3 중량%일 수 있다.The cosmetic composition may contain, in addition to the above-mentioned substances, other ingredients which can give rise to a synergistic effect on the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may comprise a material selected from the group consisting of vitamins, high molecular peptides, high molecular weight polysaccharides and sphingolipids. The cosmetic composition according to the present invention may also contain a moisturizing agent, an emollient agent, a surfactant, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjusting agent, an organic and inorganic pigment, a fragrance, . The blending amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The blending amount thereof can be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition have.
본 발명의 일측면은 1% 내지 70%(v/v) 농도의 C1~C5 알코올로 추출한 콩 추출물을 포함하는 조성물을 포함하는 식품 조성물을 제공한다.One aspect of the invention provides a food composition comprising a composition comprising a soybean extract extracted with a C 1 to C 5 alcohol at a concentration of 1% to 70% (v / v).
상기 식품 조성물의 제형은 특별히 한정되지 않으나, 예를 들어, 정제, 과립제, 드링크제, 캐러멜 등으로 제형화될 수 있다. 각 제형의 식품 조성물은 유효 성분 이외에 해당 분야에서 통상적으로 사용되는 성분들을 제형 또는 사용 목적에 따라 당업자가 어려움 없이 적의 선정하여 배합할 수 있으며, 다른 성분과 동시에 적용할 경우 상승 효과가 일어날 수 있다.The formulation of the food composition is not particularly limited, but may be formulated into tablets, granules, drinks, caramels, and the like. The food composition of each formulation may be formulated by the person skilled in the art without difficulty, depending on the purpose of formulation or use, in addition to the active ingredient, and the synergistic effect may occur when the composition is applied simultaneously with other ingredients.
상기 유효 성분의 투여량 결정은 당업자의 수준 내에 있으며, 이의 1일 투여 용량은 예를 들어 0.1mg/kg/일 내지 5000mg/kg/일, 보다 구체적으로는 50 mg/kg/일 내지 500 mg/kg/일이 될 수 있으나, 이에 제한되지 않으며, 투여하고자 하는 대상의 연령, 건강 상태, 합병증 등의 다양한 요인에 따라 달라질 수 있다.The dosage of the active ingredient is within the level of those skilled in the art and its daily dose is, for example, from 0.1 mg / kg / day to 5000 mg / kg / day, more specifically from 50 mg / kg / day, but it is not limited thereto, and may be varied depending on various factors such as the age, health condition, and complications of the subject to be administered.
본 발명의 일측면은 1% 내지 70%(v/v) 농도의 C1~C5 알코올로 추출한 콩 추출물을 포함하는 조성물을 포함하는 약학 조성물을 제공한다. 본 발명의 다른 일측면에서, 상기 약학 조성물은 탈모를 예방 또는 치료하고, 육모를 촉진하며, 모발 건강을 증진하는 효과를 나타낼 수 있다.One aspect of the present invention provides a pharmaceutical composition comprising a composition comprising a soybean extract extracted with a C 1 to C 5 alcohol at a concentration of 1% to 70% (v / v). In another aspect of the present invention, the pharmaceutical composition may exhibit an effect of preventing or treating hair loss, promoting hair growth, and enhancing hair health.
본 발명의 일측면에 따른 약학 조성물은 경구, 비경구, 직장, 국소, 경피, 정맥 내, 근육 내, 복강 내, 피하 등으로 투여될 수 있다. 경구 투여를 위한 제형은 정제(錠劑), 환제(丸劑), 연질 및 경질 캅셀제, 과립제(顆粒劑), 산제, 세립제, 액제, 유탁제(乳濁濟) 또는 펠렛제일 수 있으나, 이에 제한되는 것은 아니다. 비경구 투여를 위한 제형은 용액제, 현탁제, 유액제, 겔, 주사제, 점적제, 좌제(坐劑), 패취 또는 분무제일 수 있으나, 이에 제한되는 것은 아니다. 상기 제형은 당해 분야의 통상적인 방법에 따라 용이하게 제조될 수 있으며, 계면 활성제, 부형제, 수화제, 유화 촉진제, 현탁제, 삼투압 조절을 위한 염 또는 완충제, 착색제, 향신료, 안정화제, 방부제, 보존제 또는 기타 상용하는 보조제를 적당히 사용할 수 있다.The pharmaceutical composition according to one aspect of the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like. Formulations for oral administration may be in the form of tablets, pills, soft and hard capsules, granules, powders, granules, solutions, emulsions or pellets, It is not. Formulations for parenteral administration may be, but are not limited to, solutions, suspensions, emulsions, gels, injections, drops, suppositories, patches or spray formers. The formulations may be readily prepared according to conventional methods in the art and may be prepared by conventional means including, but not limited to, surfactants, excipients, wetting agents, emulsifying accelerators, suspending agents, salts or buffers for controlling osmotic pressure, colorants, spices, stabilizers, preservatives, Other commonly used adjuvants may be used.
본 발명의 일측면에 따른 약학 조성물의 유효 성분은 투여 받을 대상의 연령, 성별, 체중, 병리 상태 및 그 심각도, 투여 경로 또는 처방자의 판단에 따라 달라질 것이다. 이러한 인자에 기초한 적용량 결정은 당업자의 수준 내에 있으며, 이의 1일 투여 용량은 예를 들어 0.1mg/kg/일 내지 5000mg/kg/일, 보다 구체적으로는 50 mg/kg/일 내지 500 mg/kg/일이 될 수 있으나, 이에 제한되는 것은 아니다.The effective ingredients of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, route of administration, or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 5000 mg / kg / day, more specifically from 50 mg / kg / day to 500 mg / / Day, but is not limited thereto.
이하, 실시예, 비교예 및 시험예를 들어 본 발명의 구성 및 효과를 보다 구체적으로 설명한다. 그러나 이들 실시예, 비교예 및 시험예는 본 발명에 대한 이해를 돕기 위해 예시의 목적으로만 제공된 것일 뿐 본 발명의 범주 및 범위가 그에 의해 제한되는 것은 아니다.Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to examples, comparative examples and test examples. However, these examples, comparative examples and test examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto.
[실시예 1 내지 4] 10%, 20%, 50% 및 70% 에탄올 서리태 추출물의 제조[Examples 1 to 4] Preparation of 10%, 20%, 50% and 70% ethanol solids extracts
건조된 서리태 1kg을 50℃의 온도에서 각각 10%, 20%, 50% 및 70%(v/v)의 에탄올 용액 10L에 침지하여 5시간 동안 3회 환류 추출한 다음, 상온에서 12시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 7~20%이었으며, 조제된 분말은 사용시까지 저온에서 보관하였다. 1 kg of the dried seaweed was immersed in 10 L of ethanol solutions of 10%, 20%, 50% and 70% (v / v) at a temperature of 50 캜, refluxed for 3 hours for 5 hours, and left at room temperature for 12 hours . The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 7 ~ 20% and the prepared powder was stored at low temperature until use.
[실시예 5] 20% 에탄올 서목태 추출물의 제조[Example 5] Preparation of 20% ethanol Seomyeo extract
건조된 서목태 1kg을 60℃의 온도에서 20%(v/v)의 에탄올 수용액 5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 저온에서 보관하였다.1 kg of the dried seedlings were immersed in 5 L of a 20% (v / v) aqueous ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was kept at low temperature until use.
[실시예 6] 20% 에탄올 강낭콩 추출물의 제조[Example 6] Preparation of 20% ethanolic kidney bean extract
건조된 강낭콩 300g을 60℃의 온도에서 20%(v/v)의 에탄올 수용액 1.5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 저온에서 보관하였다 300 g of dried green beans was immersed in 1.5 L of a 20% (v / v) aqueous ethanol solution at a temperature of 60 ° C, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use
[실시예 7] 20% 에탄올 얼룩강낭콩 추출물의 제조[Example 7] Preparation of 20% ethanol stain kidney bean extract
건조된 얼룩강낭콩 300g을 60℃의 온도에서 20%(v/v)의 에탄올 수용액 1.5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 저온에서 보관하였다. 300 g dried bean sprouts were immersed in 1.5 L of a 20% (v / v) aqueous ethanol solution at a temperature of 60 ° C., refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was kept at low temperature until use.
[실시예 8] 20% 에탄올 콩나물콩 추출물의 제조[Example 8] Preparation of 20% ethanol bean soybean extract
건조된 콩나물콩 300g을 60℃의 온도에서 20%(v/v)의 에탄올 용액 1.5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 저온에서 보관하였다.300 g of dried bean sprouts beans were immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 ° C, refluxed for 3 hours, and left at room temperature for a certain time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was kept at low temperature until use.
[실시예 9] 20% 에탄올 황태 추출물의 제조[Example 9] Preparation of 20% ethanol extract
건조된 황태 1kg을 60℃의 온도에서 20%(v/v)의 에탄올 용액 5L에 침지하여 3시간 동안 2회 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 냉동 보관하였다. 1 kg of the dried persimmon was immersed in 5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed twice for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored frozen until use.
[실시예 10] 20% 에탄올 푸르대콩 추출물의 제조[Example 10] Preparation of 20% ethanol furry soybean extract
건조된 푸르대콩 300g을 60℃의 온도에서 20%(v/v)의 에탄올 용액 1.5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 냉동 보관하였다. 300 g of dried fleabane beans were immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored frozen until use.
[실시예 11] 20% 에탄올 울타리콩 추출물의 제조[Example 11] Preparation of 20% ethanol fence soybean extract
건조된 울타리콩 1kg을 60℃의 온도에서 20%(v/v)의 에탄올 용액 5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 저온 보관하였다.1 kg of dried fence beans was immersed in 5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use.
[실시예 12] 20% 에탄올 대두 추출물의 제조[Example 12] Preparation of 20% ethanol soybean extract
건조된 대두 1kg을 60℃의 온도에서 20%(v/v)의 에탄올 용액 5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 저온 보관하였다.1 kg of dried soybeans was immersed in 5 L of a 20% (v / v) ethanol solution at a temperature of 60 ° C., refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use.
[실시예 13] 20% 에탄올 거두 추출물의 제조[Example 13] Preparation of 20% ethanol sugar extract
건조된 거두 300g을 60℃의 온도에서 20%(v/v)의 에탄올 용액 1.5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 냉장 보관하였다.300 g of the dried reed was immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15%, and the prepared powders were refrigerated until use.
[실시예 14] 20% 에탄올 적두 추출물의 제조[Example 14] Preparation of 20% ethanolic soybean curd extract
건조된 적두 300g을 60℃의 온도에서 20%(v/v)의 에탄올 용액 1.5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 저온 보관하였다.300 g of dried red oak was immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use.
[실시예 15] 20% 에탄올 흑태 추출물의 제조[Example 15] Preparation of 20% ethanol black tea extract
건조된 흑태 300g을 60℃의 온도에서 20%(v/v)의 에탄올 용액 1.5L에 침지하여 3시간 동안 환류 추출한 다음, 상온에서 일정 시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 3~15%이었으며, 조제된 분말은 사용시까지 저온 보관하였다.300 g of the dried black tea was immersed in 1.5 L of a 20% (v / v) ethanol solution at a temperature of 60 캜, refluxed for 3 hours, and left at room temperature for a certain period of time. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 3 ~ 15% and the prepared powder was stored at low temperature until use.
[실시예 16] 서리태 부탄올 추출물의 제조[Example 16] Preparation of a frosted butanol extract
실시예 2에서 얻어진 20%(v/v) 에탄올 서리태 추출물 25g을 증류수 250ml에 녹인 후 분별 깔때기를 사용하여 n-부탄올 250ml로 2회 추출한 후 얻어진 부탄올 층을 감압 농축한 후 동결 건조하여 시료를 제조하였다. 수율은 5~15%이었으며, 제조된 시료는 사용시까지 냉동 보관하였다.25 g of the 20% (v / v) ethanol solids extract obtained in Example 2 was dissolved in 250 ml of distilled water and extracted twice with 250 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and then lyophilized to prepare a sample Respectively. The yield was 5 ~ 15%, and the prepared samples were stored frozen until use.
[실시예 17] 서목태 부탄올 추출물의 제조[Example 17] Preparation of Butanol Extract of Seomyeongbuk
실시예 5에서 얻어진 20%(v/v) 에탄올 서목태 추출물 1g을 증류수 10ml에 녹인 후 분별 깔때기를 사용하여 n-부탄올 10ml로 2회 추출한 후 얻어진 부탄올 층을 감압 농축한 후 동결 건조하여 시료를 제조하였다. 수율은 5~15%이었으며, 제조된 시료는 사용시까지 냉동 보관하였다.1 g of the 20% (v / v) ethanol ethanol extract obtained in Example 5 was dissolved in 10 ml of distilled water and extracted twice with 10 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and lyophilized to prepare a sample Respectively. The yield was 5 ~ 15%, and the prepared samples were stored frozen until use.
[실시예 18] 울타리콩 부탄올 추출물의 제조[Example 18] Preparation of bean butanol extract of fence
실시예 11에서 얻어진 20%(v/v) 에탄올 울타리콩 추출물 25g을 증류수 250ml에 녹인 후 분별 깔때기를 사용하여 n-부탄올 250ml로 2회 추출한 후 얻어진 부탄올 층을 감압 농축한 후 동결 건조하여 시료를 제조하였다. 수율은 5~15%이었으며, 제조된 시료는 사용시까지 냉동 보관하였다.25 g of the 20% (v / v) ethanol fence soybean extract obtained in Example 11 was dissolved in 250 ml of distilled water and extracted twice with 250 ml of n-butanol using a separating funnel. The obtained butanol layer was concentrated under reduced pressure, . The yield was 5 ~ 15%, and the prepared samples were stored frozen until use.
[실시예 19] 푸르대콩 부탄올 추출물의 제조[Example 19] Production of bean butanol extract of furundae
실시예 10에서 얻어진 20%(v/v) 에탄올 푸르대콩 추출물 1g을 증류수 10ml에 녹인 후 분별 깔때기를 사용하여 n-부탄올 10ml로 2회 추출한 후 얻어진 부탄올 층을 감압 농축한 후 동결 건조하여 시료를 제조하였다. 수율은 5~15%이었으며, 제조된 시료는 사용시까지 저온 보관하였다.1 g of the 20% (v / v) ethanol furda soybean extract obtained in Example 10 was dissolved in 10 ml of distilled water and extracted twice with 10 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and freeze- . The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.
[실시예 20] 황태 부탄올 추출물의 제조[Example 20] Preparation of Butanol Extract from Huang Tta
실시예 9에서 얻어진 20%(v/v) 에탄올 황태 추출물 1g을 증류수 10ml에 녹인 후 분별 깔때기를 사용하여 n-부탄올 10ml로 2회 추출한 후 얻어진 부탄올 층을 감압 농축한 후 동결 건조하여 시료를 제조하였다. 수율은 5~15%이었으며, 제조된 시료는 사용시까지 저온 보관하였다.1 g of the 20% (v / v) ethanol extract obtained in Example 9 was dissolved in 10 ml of distilled water and extracted twice with 10 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and lyophilized to prepare a sample Respectively. The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.
[실시예 21] 콩나물콩 부탄올 추출물의 제조[Example 21] Preparation of bean sprout soybean butanol extract
실시예 8에서 얻어진 20%(v/v) 에탄올 콩나물콩 추출물 1g을 증류수 10ml에 녹인 후 분별 깔때기를 사용하여 n-부탄올 10ml로 2회 추출한 후 얻어진 부탄올 층을 감압 농축한 후 동결 건조하여 시료를 제조하였다. 수율은 5~15%이었으며, 제조된 시료는 사용시까지 저온 보관하였다.1 g of the 20% (v / v) ethanol bean soybean extract obtained in Example 8 was dissolved in 10 ml of distilled water, and the mixture was extracted twice with 10 ml of n-butanol using a separating funnel. The obtained butanol layer was concentrated under reduced pressure, . The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.
[실시예 22] 대두 부탄올 추출물의 제조[Example 22] Preparation of soybean butanol extract
실시예 12에서 얻어진 20%(v/v) 에탄올 대두 추출물 25g을 증류수 250ml에 녹인 후 분별 깔때기를 사용하여 n-부탄올 250ml로 2회 추출한 후 얻어진 부탄올 층을 감압 농축한 후 동결 건조하여 시료를 제조하였다. 수율은 5~15%이었으며, 제조된 시료는 사용시까지 저온 보관하였다.25 g of the 20% (v / v) ethanol soybean extract obtained in Example 12 was dissolved in 250 ml of distilled water and extracted twice with 250 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and lyophilized to prepare a sample Respectively. The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.
[실시예 23] 강낭콩 부탄올 추출물의 제조[Example 23] Preparation of butanol extract of kidney bean
실시예 6에서 얻어진 20%(v/v) 에탄올 강낭콩 추출물 1g을 증류수 10ml에 녹인 후 분별 깔때기를 사용하여 n-부탄올 10ml로 2회 추출한 후 얻어진 부탄올 층을 감압 농축한 후 동결 건조하여 시료를 제조하였다. 수율은 5~15%이었으며, 제조된 시료는 사용시까지 저온 보관하였다.1 g of the 20% (v / v) ethanolic bean extract obtained in Example 6 was dissolved in 10 ml of distilled water and extracted twice with 10 ml of n-butanol using a separatory funnel. The obtained butanol layer was concentrated under reduced pressure and then lyophilized to prepare a sample Respectively. The yield was 5 ~ 15% and the prepared samples were stored at low temperature until use.
[비교예 1] 물을 이용한 서리태 추출물의 제조[Comparative Example 1] Preparation of a sea thyme extract using water
건조된 서리태 1kg을 100℃의 온도에서 물 10L에 침지하여 5시간 동안 3회 환류 추출한 다음, 상온에서 12시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 7~20%이었으며, 조제된 분말은 사용시까지 저온에서 보관하였다.1 kg of the dried seaweed was immersed in 10 L of water at a temperature of 100 캜, refluxed three times for 5 hours, and left at room temperature for 12 hours. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 7 ~ 20% and the prepared powder was stored at low temperature until use.
[비교예 2] 75% 에탄올을 이용한 서리태 추출물의 제조[Comparative Example 2] Preparation of a seaweed extract using 75% ethanol
건조된 서리태 1kg을 50℃의 온도에서 100%(v/v)의 에탄올 용액 10L에 침지하여 5시간 동안 3회 환류 추출한 다음, 상온에서 12시간 동안 방치하였다. 추출액을 여과하여 감압 농축하고 동결 건조하여 분말 시료를 제조하였다. 수율은 7~20%이었으며, 조제된 분말은 사용시까지 저온에서 보관하였다. 1 kg of the dried seaweed was immersed in 10 L of a 100% (v / v) ethanol solution at a temperature of 50 캜, refluxed for 3 hours for 5 hours, and left at room temperature for 12 hours. The extract was filtered, concentrated under reduced pressure and lyophilized to prepare a powder sample. The yield was 7 ~ 20% and the prepared powder was stored at low temperature until use.
[비교예 3] 100% 에탄올을 이용한 서리태 추출물의 제조[Comparative Example 3] Preparation of a seaweed extract using 100% ethanol
100%(v/v)의 에탄올을 사용하였다는 것을 제외하고는 실질적으로 비교예 2와 동일한 방법으로 서리태 추출물을 수득하였다. 수율은 7~20%이었으며, 조제된 분말은 사용시까지 저온에서 보관하였다. A seared extract was obtained in substantially the same manner as in Comparative Example 2, except that 100% (v / v) ethanol was used. The yield was 7 ~ 20% and the prepared powder was stored at low temperature until use.
[시험예 1] 모유두 세포 증식 촉진 효과 평가[Test Example 1] Evaluation of promoting effect of proliferation of dermal papilla cells
쥐수염 모유두세포(Rat Vibrissa dermal papillae cell; 온도 민감 T 항원을 인코딩한 폴리오마바이러스 라지 T 유전자(polyomavirus large T gene)를 PVG 쥐수염 모유두세포(vibrissa dermal papilla cell)에 트랜스펙션하여 만든 PVGDPLTt sa 6이다. 이하, DP 세포라 한다.)를 이용하여 콩 추출물의 모유두 세포 증식 촉진 효과를 평가하였다.Rat Vibrissa dermal papillae cell (PVGDPLTT sa) transfected with polyomavirus large T gene encoding temperature-sensitive T antigen into PVG mouse vibrissa dermal papilla cell 6. Hereinafter, it is referred to as a DP cell) to evaluate the promoting effect of the soybean extract on the development of the papilla cell.
먼저 DP 세포를 96-웰 플레이트에 한 웰 당 약 104 세포/웰의 농도가 되도록 준비하였다. 실시예 1 내지 4 및 비교예 1 내지 3을 DMSO(Dimethyl sulfoxide) 또는 에탄올에 각각 용해시킨 후, 5% FBS 세럼 농도를 가지는 DMEM 배지로 순차적 희석(serial dilution)하여 최종 농도가 10ppm이 되도록 하였다. 실시예 1 내지 4 및 비교예 1 내지 3이 첨가된 배지 200㎕를 각각 웰 당 첨가한 후, 37℃, 5% CO2 배양기에서 약 3일간 배양하였다. 2㎎/㎖의 농도로 조제된 MTT 용액을 각 웰 당 50㎕씩 첨가한 후, 동일한 배양기에서 4시간 동안 방치하였다. MTT 시약이 세포 내 미토콘드리아에 흡수되었다고 판단되면 상등액을 제거하고, DMSO를 150㎕ 첨가한 다음, 10분간 교반하여 균일하게 프로마잔(formazan) 염색(dye)을 녹여내었다. 그런 다음 ELISA 리더를 이용하여 515nm 파장에서 O.D. 값을 측정하고, 미리 만들어둔 표준 곡선에 맞추어 세포 수를 측정하였다. 측정한 세포 수를 기초로 실시예 1 내지 4 및 비교예 1 내지 3의 모유두 세포 증식 촉진능(%)을 계산하고 그 결과를 아래 표에 나타내었다.DP cells were first prepared to a concentration of about 10 4 cells / well per well in a 96-well plate. Examples 1 to 4 and Comparative Examples 1 to 3 were dissolved in DMSO (dimethyl sulfoxide) or ethanol, and then serial dilution was performed with DMEM medium having a 5% FBS serum concentration to obtain a final concentration of 10 ppm. 200 占 퐇 of the medium to which Examples 1 to 4 and Comparative Examples 1 to 3 were added was added to each well and cultured at 37 占 폚 in a 5% CO 2 incubator for about 3 days. MTT solution prepared at a concentration of 2 mg / ml was added in an amount of 50 占 퐇 / well, followed by incubation in the same incubator for 4 hours. When it was judged that the MTT reagent was absorbed into the intracellular mitochondria, the supernatant was removed, 150 μl of DMSO was added, and the mixture was stirred for 10 minutes to uniformly dissolve the formazan dye. The OD value at 515 nm was measured using an ELISA reader, and the number of cells was measured according to a standard curve prepared beforehand. Based on the measured cell numbers, the ability of the examples 1 to 4 and the comparative examples 1 to 3 to promote the proliferation of the dermal papilla cells (%) was calculated and the results are shown in the table below.
세포 증식 촉진능(%)Dairy cattle
Cell proliferation promoting ability (%)
상기에서 볼 수 있듯이, 물로 추출한 비교예 1과 각각 75%(v/v), 100%(v/v)로 추출한 비교예 2 및 3에 비해 실시예 1 내지 4의 모유두 세포 증식 촉진 효과가 더 우수하였다. 특히 20% 에탄올로 추출한 콩 추출물인 실시예 2의 모유두 세포 증식 촉진 효과가 가장 우수하였다. 따라서 알코올로 추출한 콩 추출물은 모유두 세포 증식을 촉진하여 탈모를 방지하고 육모를 촉진하며 나아가 모발 건강을 증진하는 뛰어난 효과가 있음을 알 수 있다.As can be seen from the above, compared with Comparative Example 1 extracted with water and Comparative Examples 2 and 3 extracted with 75% (v / v) and 100% (v / v) . Especially, the effect of promoting the growth of the papilla cells of Example 2, which is a soybean extract extracted with 20% ethanol, was the best. Therefore, the soybean extract extracted with alcohol promotes the proliferation of the papillary cells, thereby preventing hair loss, accelerating hair growth, and further enhancing hair health.
[시험예 2] 모세혈관 확장 효과 평가[Test Example 2] Capillary expansion effect evaluation
K+ 채널이 열리면 미토제네시스(mitogenesis)가 활성화되고 혈관 평활근(vascular smooth muscle)이 이완되면서 혈관이 확장된다. 한편, K+ 채널이 차단되면 NIH3T3 섬유아세포(fibroblast)의 증식이 저해된다고 알려져 있다. 따라서 시료 처리에 의해 NIH3T3 섬유아세포의 성장이 촉진되면, K+ 채널이 열린다고 볼 수 있고, 나아가 혈관 확장이 촉진된다고 볼 수 있다.When the K + channel is opened, mitogenesis is activated and the vascular smooth muscle relaxes and blood vessels expand. On the other hand, it is known that inhibition of NIH3T3 fibroblast proliferation is inhibited when K + channel is blocked. Therefore, when the growth of NIH3T3 fibroblasts is promoted by the sample treatment, it can be seen that the K + channel is opened and further the vasodilation is promoted.
먼저 NIH3T3 섬유아세포를 24-웰 배양 플레이트에 104 세포/웰의 농도로 시드(seed)하고, 10% FCS(fetal calf serum) 및 2mM L-글루타민을 포함하며 항생제를 포함하지 않는 DMEM 배지에서 24시간 배양하였다. 이후 배양 배지를 각각 실시예 1 내지 4 및 비교예 1과 3이 첨가된 배지로 교체하고 72시간 배양하였다. 실시예 1 내지 4 및 비교예 1과 3이 첨가된 배지 제조 방법은 시험예 1과 같다. 배지를 제거한 후 PBS(Ca2+/Mg2+ 없음)로 세척하고, 0.5ml TE(trypsin-ethylenediamine tetraacetic acid)로 처리한 후 배양하였다. 10% FCS가 포함된 DMEM를 가하고 세포를 취한 다음 EP 튜브에 담아 6000rpm에서 원심 분리하였다. 상등액을 제거하고 남은 세포액을 100㎕의 PBS에 현탁한 후 혈구 계산기(hemocytometer)를 사용하여 세포 수를 측정하였다. 이를 기초로 실시예 1 내지 4 및 비교예 1과 3의 모세혈관 확장능을 계산하여 아래 표에 나타내었다.First, NIH3T3 fibroblasts were seeded at a concentration of 10 4 cells / well in a 24-well culture plate and cultured in DMEM medium containing 10% FCS (fetal calf serum) and 2 mM L-glutamine, Time. Then, the culture medium was replaced with the medium to which Examples 1 to 4 and Comparative Examples 1 and 3 were added, respectively, and cultured for 72 hours. The medium preparation methods to which Examples 1 to 4 and Comparative Examples 1 and 3 are added are the same as those of Test Example 1. After the medium was removed, the cells were washed with PBS (Ca 2+ / Mg 2+ free), treated with 0.5 ml TE (trypsin-ethylenediamine tetraacetic acid), and cultured. DMEM containing 10% FCS was added and cells were taken and centrifuged at 6000 rpm in an EP tube. The supernatant was removed, and the remaining cell lysate was suspended in 100 μl of PBS, and then the number of cells was measured using a hemocytometer. Based on this, the capillary vasodilatability of Examples 1 to 4 and Comparative Examples 1 and 3 was calculated and shown in the table below.
확장능(%)Capillary
Extensibility (%)
상기에서 볼 수 있듯이, 물과 75%(v/v)로 각각 추출한 비교예 1 및 3에 비해 실시예 1 내지 4의 모세혈관 확장 효과가 우수하였다. 특히 20% 에탄올로 추출한 콩 추출물인 실시예 2의 모세혈관 확장 효과가 가장 우수하였다. 따라서 알코올로 추출한 콩 추출물은 두피 모세혈관의 혈액 순환을 개선하여 모발 건강을 증진시키고 탈모의 진행을 억제하며 육모를 촉진함에 있어 뛰어난 효과를 가짐을 알 수 있다.As can be seen from the above, the capillary vasodilating effect of Examples 1 to 4 was superior to that of Comparative Examples 1 and 3, which were respectively extracted with water and 75% (v / v). Especially, the capillary vasodilating effect of Example 2, which is a soybean extract extracted with 20% ethanol, was the best. Therefore, the soybean extract extracted with alcohol can improve blood circulation of scalp capillary blood vessels, thereby improving hair health, inhibiting the progress of hair loss and promoting hair growth.
[시험예 3] 모발 성장 촉진 효과 평가[Test Example 3] Evaluation of hair growth promoting effect
실시예 2와 4를 이용하여 마우스의 모발 성장 촉진 효과를 평가하였다. 먼저 생후 47～53일된 마우스(C57BL/6)의 등 부위 털을 일렉트릭 크립퍼(electric clipper)로 제거하였다. 상기 연령의 마우스 모발은 휴지기에 들어선 상태이므로 바로 성장하지 않는다는 특징을 가진다. 그 중 등 부위 피부가 양호한 마우스들을 골라 한 군당 5~7마리의 마우스가 포함되도록 3개의 군으로 나눈 후, 별도의 케이스에서 사육하였다. 실시예 2와 4가 각각 0.6%로 포함되도록 한 시리얼 사료를 식이를 통하여 자유 섭취하도록 하였다. 콩 추출물을 포함하지 않은 시리얼 사료를 섭취한 군을 대조군으로 하였다. 23일 후 각 군의 제모 부위 변화를 관찰하고 그 사진을 도 1에 나타내었다. 또한 제모 부위에 새로 성장한 각 마우스의 모발을 일렉트릭 크립퍼로 수거하여 무게를 측정하였다. 그 결과를 아래 표에 나타내었다.The hair growth promoting effect of the mice was evaluated using Examples 2 and 4. First, the dorsal hairs of the mice (C57BL / 6) at 47 to 53 days of age were removed with an electric clipper. Since the mouse hair of this age is in a resting state, it does not grow immediately. Among them, mice with good back skin were selected and divided into three groups so that 5 to 7 mice were included in each group, and then they were raised in a separate case. The cereal feeds in which Examples 2 and 4 were included at 0.6% were freely taken through diets. The control group was fed with cereal feed containing no soybean extract. After 23 days, the change of the epilation area of each group was observed and the photograph is shown in Fig. In addition, hair of each newly-developed mouse on the hair removal area was collected by electric creepers and weighed. The results are shown in the table below.
상기에서 볼 수 있듯이, 에탄올로 추출한 콩 추출물은 마우스 모발의 성장을 촉진시켰다. 이는 도 1에 나타나는 외관 및 표 3의 모발 무게에서도 알 수 있다. 특히, 20% 에탄올로 추출한 콩 추출물의 모발 성장 촉진 효과가 매우 뛰어났다. 따라서 알코올로 추출한 콩 추출물은 모발 성장 촉진 효과가 우수함을 알 수 있다.As can be seen from the above, the soybean extract extracted with ethanol promoted the growth of mouse hair. This can be seen from the appearance shown in Fig. 1 and the hair weight in Table 3. Especially, soybean extract extracted with 20% ethanol showed excellent hair growth promoting effect. Therefore, the soybean extract extracted with alcohol has excellent hair growth promoting effect.
아래에 상기 조성물의 제형예를 설명하나, 이는 본 발명을 한정하는 것이 아니라 단지 구체적으로 설명하고자 함이다.Formulation examples of the composition are described below, but are not intended to limit the invention, but merely to illustrate them.
[제형예 1] 연질 캅셀제[Formulation Example 1] Soft capsule
서리태 추출물 100 mg, 대두 추출물 50 mg, 대두유 180 mg, 팜유 2 mg, 팜경화유 8 mg, 황납 4 mg 및 레시틴 6 mg을 혼합하고, 통상의 방법에 따라 1 캡슐당 400 mg씩 충진하여 연질 캅셀제를 제조한다.A soft capsule was prepared by mixing 100 mg of a safflower extract, 50 mg of a soybean extract, 180 mg of soybean oil, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow pearls and 6 mg of lecithin, .
[제형예 2] 정제[Formulation Example 2] Tablets
울타리콩 추출물 100 mg, 포도당 100 mg, 전분 96 mg 및 마그네슘 스테아레이트 4 mg을 혼합하고 30% 에탄올을 40 mg 첨가하여 과립을 형성한 후, 60℃에서 건조하고 타정기를 이용하여 정제로 타정한다.100 mg of fence bean extract, 100 mg of glucose, 96 mg of starch and 4 mg of magnesium stearate are mixed and 40 mg of 30% ethanol is added to form granules. The granules are dried at 60 ° C. and tableted by tabletting.
[제형예 3] 드링크제[Formulation Example 3] Drinking agent
서목태 추출물 100 mg, 포도당 10 g, 구연산 2 g 및 정제수 187.8 g을 혼합하고 병에 충진한다. 내용물의 최종 용량은 200 ml로 한다.100 mg of Seomunghae extract, 10 g of glucose, 2 g of citric acid and 187.8 g of purified water are mixed and filled into a bottle. The final volume of the contents should be 200 ml.
[제형예 4] 건강 식품[Formulation Example 4] Health food
서리태 추출물......................... 1000 ㎎ Seaweed extract ......................... 1000 mg
비타민 혼합물 Vitamin mixture
비타민 A 아세테이트......................70 ㎍ Vitamin A Acetate ...................... 70 ㎍
비타민 E .............................. 1.0 ㎎ Vitamin E .............................. 1.0 mg
비타민 B1............................. 0.13 ㎎ Vitamin B1 ............................. 0.13 mg
비타민 B2 ............................ 0.15 ㎎ Vitamin B2 ............................ 0.15 mg
비타민 B6.............................. 0.5 ㎎ Vitamin B6 .............................. 0.5 mg
비타민 B12............................. 0.2 ㎍ Vitamin B12 ............................. 0.2 g
비타민 C................................ 10 ㎎ Vitamin C ................................ 10 mg
비오틴.................................. 10 ㎍ Biotin .................................. 10 μg
니코틴산아미드......................... 1.7 ㎎ Nicotinic acid amide ....................... 1.7 mg
엽산.................................... 50 ㎍ Folic acid .................................... 50 ㎍
판토텐산 칼슘.......................... 0.5 ㎎ Calcium pantothenate .......................... 0.5 mg
무기질 혼합물 Mineral mixture
황산제1철.............................. 1.75 ㎎ Ferrous sulfate .............................. 1.75 mg
산화아연............................... 0.82 ㎎ Zinc oxide ............................... 0.82 mg
탄산마그네슘........................... 25.3 ㎎ Magnesium carbonate ........................... 25.3 mg
제1인산칼륨.............................. 15 ㎎ Potassium Phosphate .............................. 15 mg
제2인산칼슘.............................. 55 ㎎ Secondary calcium phosphate .............................. 55 mg
구연산칼륨............................... 90 ㎎ Potassium citrate ............................... 90 mg
탄산칼슘................................ 100 ㎎ Calcium carbonate ................................ 100 mg
염화마그네슘............................ 24.8 ㎎ Magnesium chloride ............................ 24.8 mg
상기의 비타민 및 미네랄 혼합물의 조성비는 비교적 건강 식품에 적합한 성분을 혼합 조성하였지만, 그 배합비를 임의로 변형 실시하여도 무방하며, 통상의 건강 식품 제조방법에 따라 상기의 성분을 혼합한 다음, 과립을 제조하고, 통상의 방법에 따라 건강 식품 조성물 제조에 사용할 수 있다.Although the composition ratio of the above-mentioned vitamin and mineral mixture is relatively mixed with the ingredients suitable for health food, the compounding ratio may be arbitrarily changed. The above ingredients are mixed according to the ordinary health food manufacturing method, And can be used in the manufacture of a health food composition according to a conventional method.
[제형예 5] 크림[Formulation Example 5] Cream
아래 표에 기재된 조성에 따라 통상적인 방법으로 크림을 제조한다.Creams are prepared by conventional methods according to the compositions listed in the table below.
본 발명이 속한 기술 분야에서 통상의 지식을 가진 자라면 상기 내용을 바탕으로 본 발명의 범주 내에서 다양한 응용 및 변형을 행하는 것이 가능할 것이다.It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
A composition for oral hair loss prevention or promotion of hair growth, comprising an extract of a sea tangle extracted with ethanol at a concentration of 12% to 25% (v / v) as an active ingredient.
A composition for oral administration for enhancing hair health, which comprises, as an active ingredient, a sea tangle extract extracted with ethanol at a concentration of 12% to 25% (v / v).
조성물은 조성물의 전체 중량을 기초로 0.001 내지 90 중량%의 서리태 추출물을 포함하는 조성물.
3. The method according to claim 1 or 2,
Wherein the composition comprises from 0.001 to 90% by weight of the perfume extract, based on the total weight of the composition.
조성물은 두피의 혈액 순환을 개선하여 모근에 영양을 공급하는 조성물.
3. The method according to claim 1 or 2,
The composition improves the blood circulation of the scalp and nourishes the hair follicle.
A food composition comprising the composition according to claims 1 or 2.
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| KR20060055017A (en) * | 2004-11-17 | 2006-05-23 | 최양원 | A manufacturing process of epilation for acceleration and depilation prevention |
| KR20100080991A (en) * | 2009-01-05 | 2010-07-14 | (주)아모레퍼시픽 | Hair cosmetic composition for strengthening hair root |
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| KR20060055017A (en) * | 2004-11-17 | 2006-05-23 | 최양원 | A manufacturing process of epilation for acceleration and depilation prevention |
| KR20100080991A (en) * | 2009-01-05 | 2010-07-14 | (주)아모레퍼시픽 | Hair cosmetic composition for strengthening hair root |
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