KR101957847B1 - Composition for skin whitening comprising kadsura coccinea extract - Google Patents

Abstract

The present invention discloses a composition for skin whitening comprising an extract of black algae as an active ingredient.

Description

Technical Field [0001] The present invention relates to a composition for skin whitening,

The present invention relates to a composition for skin whitening comprising a black algae extract.

The skin color of a person is determined by various factors such as the activity of the melanocyte making the melanin pigment (melanocyte), the distribution of blood vessels, the thickness of the skin, or the presence of pigments such as carotenoids and bilirubin. Among them, the formation of melanin, which is a black pigment produced in melanocytes by the action of various enzymes such as tyrosinase, is one of the most important factors determining the color of the skin.

Melanin present in the skin is an important function for protecting the body from ultraviolet light. However, when it exists in excess, melanin causes skin pigmentation such as spots, freckles, dots, and black spots, as well as promotes skin aging, It is also known to act as.

Various substances known to exhibit skin whitening effects, such as ascorbic acid, kojic acid, arbutin, hydroquinone or derivatives thereof, have been used to treat or alleviate skin pigmentation due to melanin overproduction, And the skin irritation is strong, so that there is a limitation in its use.

Korean Patent Publication No. 10-2004-0092781 (November 04, 2004) Specification

The present invention aims to provide a composition having excellent skin whitening effect. The present invention also provides a cosmetic composition having excellent skin whitening effect.

One aspect of the present invention provides a composition for skin whitening comprising an extract of black algae as an active ingredient.

Another aspect of the present invention provides a cosmetic composition for skin whitening comprising a black algae extract as an active ingredient.

The composition according to the present invention can contain melanin, inhibiting the production of melanin and exhibiting excellent skin whitening effect, and can be freely used even those having sensitive skin with little irritation to the skin.

Fig. 1 is a graph showing the melanin production inhibitory activity of the black algae extract.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal including a human, and is a concept of the broadest notion including not only a tissue covering a body surface such as a face or a body but also a scalp and a hair. In the present specification, "sensitive skin" is defined as a skin which is less resistant to external stimuli and is susceptible to adverse reactions such as stinging, burning, burning, pruritus, flushing, dryness, The causes of such sensitive skin include congenital skin characteristics, family history, medical history, misuse of cosmetics, living environment or stress.

As used herein, the term "extract" is a broad concept that encompasses all of the substances obtained by extracting components of natural products, regardless of the type of extraction method, extraction solvent, extracted component or extract.

One aspect of the present invention provides a composition for skin whitening comprising an extract of black algae as an active ingredient. Black roe extract inhibits melanin production, so that a composition containing the same can have an excellent skin whitening effect. Specifically, the composition according to one aspect of the present invention enhances brightness and uniformity of the entire skin color and suppresses hyperpigmentation by incorporating a black algae extract as an active ingredient. In addition, since the extract of black bean has a hypoallergenic property that gives little irritation to the skin, the composition containing it can be freely used even by those having sensitive skin. This is an excellent effect of improving high skin irritation, one of the disadvantages of existing skin whitening substances.

According to one aspect of the present invention, the black-tiger (Kadsura coccinea (Lem.) AC Smith) is sometimes referred to as "posterior omisina. &Quot; In one aspect of the invention, the Black Roast Calli extracts include, but are not limited to, leaves, roots, roots, flowers, fruits or seeds of black roots. In another aspect of the present invention, the Black Roast Calli extract comprises a black roast extract obtained by extracting at least one selected from the group consisting of water, an organic solvent, and a mixture of water and an organic solvent. More specifically, ≪ / RTI > The organic solvent includes, but is not limited to, at least one selected from the group consisting of alcohol, acetone, hexane, ether, ethyl acetate, diethyl ether, ethyl methyl ketone and chloroform. Wherein the alcohol comprises a C ₁ to C ₅ lower alcohol and the C ₁ to C ₅ lower alcohol is selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol But is not limited thereto.

The composition according to one aspect of the present invention may comprise 0.001 to 80% by weight, specifically 0.01 to 50% by weight, more specifically 0.1 to 10% by weight, of the Black Curd Calligraphy extract based on the total weight of the composition. When it is contained in the above weight percentage, it is not only suitable for exhibiting the intended skin whitening effect of the present invention but also can satisfy both the stability and safety of the composition, and may be suitably included in the above range in terms of cost effectiveness.

One aspect of the present invention provides a cosmetic composition comprising a black algae extract as an active ingredient. The cosmetic composition may exhibit skin whitening effect and specifically improve or prevent skin pigmentation such as spots, freckles, dots, black spots, and other skin irritations. In particular, since the cosmetic composition has low irritation to the skin, it can be used regardless of the skin type of the user. The skin type includes sensitive skin or dry skin.

The cosmetic composition according to the present invention may be provided in all formulations suitable for topical application. For example, it may be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, so long as they do not impair the main effect. Specifically, the cosmetic composition according to the present invention may further comprise kojic acid, arbutin or ascorbic acid derivative which can enhance skin whitening effect. The cosmetic composition according to the present invention may further comprise a moisturizing agent, an emollient agent, a surfactant, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a fragrance, have. The compounding amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The amount thereof may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, have.

One aspect of the present invention provides a pharmaceutical composition comprising an extract of Black Curcuma longa as an active ingredient. The pharmaceutical composition may exhibit a skin whitening effect and specifically improve or treat skin pigmentation such as spots, freckles, dots, black spots, and other skin irritations.

The pharmaceutical composition according to one aspect of the present invention may be administered orally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

Formulations for oral administration may be in the form of tablets, pills, soft and hard capsules, granules, powders, granules, solutions, emulsions or pellets, It is not. These formulations may contain, in addition to the active ingredient, a diluent such as lactose, dextrose, sucrose, mannitol, sorbitol, cellulose or glycine, a lubricant such as silica, talc, stearic acid or polyethylene glycol, or a binder such as magnesium aluminum Silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose or polyvinylpyrrolidone). And may optionally contain pharmaceutical additives such as disintegrants, absorbents, colorants, flavors or sweeteners.

Formulations for parenteral administration may be, but are not limited to, injections, drips, lotions, ointments, gels, creams, suspensions, emulsions, suppositories, patches or sprayings.

The effective ingredients of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, route of administration, or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

One aspect of the present invention provides a food composition comprising a black algae extract as an active ingredient. The food composition comprises a preferred food composition or a health food composition. The food composition containing the black algae extract as an active ingredient according to another aspect of the present invention may exhibit a skin whitening effect and specifically may improve or prevent skin pigmentation such as spots, freckles, dots, have.

The formulation of the food composition is not particularly limited, but may be formulated into, for example, tablets, granules, powders, liquid preparations such as a drink, gel, bars and the like. The food composition of each formulation can be blended with the ingredients commonly used in the field in addition to the active ingredient without difficulty by those skilled in the art depending on the purpose of formulation or use, and synergistic effect can be obtained when the composition is applied simultaneously with other ingredients.

The dosage of the active ingredient is within the level of those skilled in the art and its daily dose is, for example, from 0.1 mg / kg / day to 5000 mg / kg / day, more specifically from 50 mg / kg / day, but it is not limited thereto, and may be varied depending on various factors such as the age, health condition, and complication of the subject to be administered.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to Examples and Experimental Examples. However, these examples and experimental examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

[Examples] Preparation of black algae extract

Black algae extract was prepared by a conventional method in the art using black algae. Specifically, 1 kg of Black Roe was washed, dried at 60 DEG C for 24 hours, and cut. This was repeatedly extracted three times at 70 to 75 DEG C for 1 hour with 95% ethanol. After filtration, 113 g of black radish extract was obtained.

[Experimental Example 1] Evaluation of inhibitory effect on melanin formation

In order to evaluate the melanin formation inhibitory effect of the black algae extract, the following experiment was carried out using the black algae extract of Example.

First, mouse melanoma (Murine melanoma) (Melan-a ) a cell under the after FBS containing the inoculated RPMI640 or equal than 1x10 ⁵ per well in 12-well plates in medium with seongjangryeok pieces, 5% CO _2, 37 ℃ The cells were cultured until they were confluent to about 80% of the bottom of the wells. Under then remove the culture media, each of 1, 5 and 10 ㎍ / heukno No. extract of ml and 0.5, and then replaced with a 1 and the medium is diluted in 2 mM arbutin 5% CO _2, 37 ℃ were incubated for 7 days. The concentration range of the sample was determined through toxicity experiments. Cells from which the medium had been removed were washed with PBS and treated with trypsin to recover the cells. The recovered cells were centrifuged at 5,000 to 10,000 rpm for 10 minutes, and the supernatant was removed to obtain a pallet. The resulting cell pellet was dried at 60 ° C., and 100 μl of 1 M NaOH containing 10% DMSO or cell lysis buffer was added thereto to obtain intracellular melanin in a 60 ° C. thermostat. The absorbance of the obtained solution was measured by a microplate reader at 490 nm and the amount of protein was determined by Bradford method and the amount of melanin per cell constant number or the amount of melanin per constant protein was determined. The melanogenesis activity (%) of each concentration of black nose and arbutin relative to the control untreated sample is shown in the table below and in FIG.

Control group Arbutin (mM) Black roe extract (㎍ / ml) 0.5 One 2 One 5 10 Melanin production (%) 100 92.07 80.96 70.29 110.71 79.94 61.92

As can be seen from the above results, the black algae extract has an inhibitory effect on melanin production, and this effect is superior to arbutin, which is known as a conventional skin whitening substance. As a result, it can be seen that the composition containing the black algae extract has an excellent skin whitening effect by inhibiting melanin production.

[Experimental Example 2] Evaluation of degree of skin irritation

In order to evaluate the skin irritation level of black algae extract, the following experiment was conducted in comparison with kojic acid, which is known as a conventional skin whitening substance.

Fifteen subjects with sensitive skin sensitive to stinging and burning by stimulation were selected, and 0.5 ml of each of Kozan acid (purchased from YM Chemical) and the black roots extract of the examples were randomly applied to the left and right sides of the face, The degree of warmth was rated as 3.0 out of 0.1 points. The evaluation criteria and evaluation results are as follows.

Kojic acid Black roe extract Stinginess 0.85 0.35 Burning 0.50 0.55 Average 0.68 0.45

0 to 0.4: No or almost no stimulation

0.5 ~ 1.0: Feeling slight irritation

1.1 to 2.0: Feeling a moderate degree of irritation

2.1 ~ 3.0: Feeling severe irritation

As can be seen from the above results, in the area coated with kojic acid, there was almost no feeling of stinging and burning, whereas in the area coated with black roe extract, burning and burning were hardly felt. In other words, it can be said that the extract of black algae can be freely used for sensitive skin because it does not irritate the skin unlike the known skin whitening substance Kojisan.

Formulation examples of compositions according to one aspect of the present invention are described below, but may be applied to various other formulations, which are not intended to be limiting but merely illustrative of the invention.

[Formulation Example 1] Lotion

The lotion is prepared according to a conventional method with the composition shown in the following table.

ingredient Content (% by weight) Black roe extract 3.0 L-ascorbic acid-2-phosphate magnesium salt 1.0 Water soluble collagen (1% aqueous solution) 1.0 Sodium citrate 0.1 Citric acid 0.05 1,3-butylene glycol 3.0 Purified water Balance

[Formulation Example 2] Cream

Creams are prepared according to a conventional method with the composition shown in the following table.

ingredient Content (% by weight) Black roe extract 1.0 Polyethylene glycol monostearate 2.0 Glycerin monostearate 5.0 Cetyl alcohol 4.0 Squalene 3.0 Tri-2-ethylhexane glyceryl 6.0 Sphingoglycolipids 1.0 1,3-butylene glycol 7.0 Purified water Balance

[Formulation Example 3] Soft capsule

A soft capsule was prepared by mixing 100 mg of black radish extract, 50 mg of soybean extract, 180 mg of soybean oil, 50 mg of red ginseng extract, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow pearls and 6 mg of lecithin according to a conventional method .

[Formulation Example 4] Tablets

100 mg of black algae extract, 50 mg of soybean extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate are mixed and ethanol is added to form granules, followed by drying and tableting.

[Formulation Example 5] Health drinks

Black roe extract .................... 1000 mg

Citric acid ................................ 1000 mg

Oligosaccharides ............................... 100 g

Taurine ................................... 1 g

Purified water .................................. Remaining amount

The above components are mixed according to a conventional health drink manufacturing method, and the mixture is stirred and heated at about 85 캜 for about 1 hour, and then the prepared solution is sterilized by filtration.

Claims (5)

As the active ingredient,
A composition for skin whitening, which is suitable for sensitive skin.
The method according to claim 1,
Wherein the composition comprises 0.001% to 80% by weight of a black and white tincture extract based on the total weight of the composition.
The method according to claim 1,
Wherein the composition inhibits melanin production.
4. The method according to any one of claims 1 to 3,
Wherein the composition is a cosmetic composition.
delete

Images