KR101068808B1 - Composition for Whitening Containing Extract of Delonix regia - Google Patents

Abstract

The present invention relates to a whitening composition containing the extract of phoenix tree ( Delonix regia ) as an active ingredient, more specifically, to a cosmetic composition containing phoenix tree extract and a dermatologically acceptable carrier and phoenix tree extract and food It relates to a food composition containing a carrier acceptable as.

The phoenix tree extract according to the present invention can be usefully used as a composition for skin whitening because it exhibits a whitening effect by inhibiting the production of melanin without causing toxicity to cells.

Phoenix, whitening

Description

Composition for Whitening Containing Extract of Delonix regia containing phoenix tree extract as an active ingredient

The present invention relates to a whitening composition containing phoenix tree extract as an active ingredient, and more particularly, to a cosmetic composition containing phoenix tree extract and a dermatologically acceptable carrier, and to a pharmaceutically acceptable carrier. It relates to a food composition containing.

Skin is a very important tissue that protects the human body while directly contacting the external environment and has biochemical and physical functions. The skin is divided into three types: the epidermis, the dermis, and the hypodermis. Melanin is a pigment present in the epidermal layer of the skin. It is made from melanocytes in the basal layer of the epidermis and migrates to epidermal cells called keratinocytes. There are no enzymes in the body that break down melanin, but they are removed by falling off the skin as keratinocytes fall off the epidermis.

In the melanin synthesis process, melanocyte vesicles in melanocytes are first converted into tyrosine through dopa (DOPA) by an enzyme called tyrosinase and then into dopaquinone (DOPAquinone). Dopachrome produces melanin, a blackish brown copolymer. The melanin thus produced is transferred to keratinocytes through the melanosomes, where it is lost to the surface of the skin along with the keratinization process for about 28 days.

Melanin absorbs the light energy of sunlight UV to protect skin organs under the dermis from UV damage, and it protects the skin from external harmful factors such as trapping harmful oxygen and free radicals generated in skin skin. Play a role. In addition, the skin color of a person is determined according to the amount of melanin in the skin cells, and those with a lot of melanin pigment have brown or black skin, while those with a small amount of melanin have white skin.

When abnormally low melanin is produced, skin lesions such as vitiligo are caused, and conversely, if melanin accumulates due to excessive synthesis or keratinization due to excessive daylight, hormonal changes, inflammation, and drugs, damage to the skin can occur. In addition to the skin pigments are deposited to form blemishes, freckles, and even from these lesions can cause skin cancer. Therefore, in order to prevent such a pigmentation phenomenon, it is necessary to inhibit a part of melanogenesis process to reduce melanin production.

Today, Asian women prefer white and clean skin and make it an important standard of beauty. Therefore, the development of whitening agent for treatment of abnormal skin pigmentation and satisfying beauty needs is being actively developed. At present, in the development of a whitening agent for skin beauty, a method of reducing the generated melanin pigment by decolorization and a method of inhibiting the activity of tyrosinase, an enzyme that forms melanin pigment, are known. However, whitening agents using tocopherols and vitamins used to reduce the melanin pigment are known to have a slight decolorizing effect of the melanin pigment. Therefore, many studies have been conducted on inhibitors that inhibit the production of melanin pigment.

In the conventional cosmetic field, as a whitening component, for example, substances that inhibit tyrosinase enzyme activity such as kojic acid, arbutin, hydroquinone, vitamin-C (L-Ascorbic acid) and Their derivatives and various plant extracts have been used.

However, the whitening components are deteriorated due to poor stability in the prescription system, or the use thereof is limited due to the occurrence of off-flavor, efficacy at the biological level, unclear effect and safety problems. Kojic acid inhibits enzymatic activity by adsorbing copper ions present in the active site of tyrosinase, but has been discontinued as a raw material for cosmetics because of its instability, skin side effects and recent animal experiments. Vitamin-C and its derivatives are difficult to use as raw materials for cosmetics due to their oxidative instability. Hydroquinone has excellent whitening effect on skin, but it causes allergies, toxicity to melanin producing cells, permanent depigmentation to skin, etc. It is highly irritating to skin, and is recently prescribed as a carcinogenic substance, so its use is banned and only a limited concentration is allowed in each country. In addition, arbutin is a derivative in which glucopyranoside is combined with hydroquinone, and has little side effects when using hydroquinone and inhibits the synthesis of melanin pigments without toxicity to humans, thereby increasing melanin pigmentation. Applicability has been suggested as a therapeutic agent for skin diseases, but it has a disadvantage of being partially degraded by skin enzymes.

In addition to these whitening substances, research has been conducted to find whitening active ingredients in natural products, especially plants. Among them, white skin (Japanese Patent Application Laid-Open No. 55-44375, Small 64-26507, Small 64-83009, Pyeong 1-25687 No. 5-139950, Korean Patent Publication Nos. 92-002109, 97-021273, Licorice (Japanese Patent Laid-Open No. 60-214721, No. 60-214728, No. 63-23809, No. 64- 63506, Hei 1-149706, and Korean Patent Nos. 92-002109, 97-025601), Peony (Japanese Patent Laid-Open No. 61-246109 and Korean Laid-Open Patent No. 92-002111), Cinnamon Patent Nos. 63-30403, Hep. 5-139954), Gosam (Japanese Patent Laid-Open No. 64-26507 and Korean Patent Laid-Open No. 92-002110), Brown root (Japanese Laid-Open Patent Nos. 60-214727, Small 64-16709 ), Angelica (Japanese Patent Laid-Open No. 56-92211, Hei 4-26610), Mokdanpi (Japanese Laid-Open Patent No. 60-214721, No. 61-50915, No. 61-246109), Banha (Japanese Laid-Open Patent Publication) Pyeong 2-207028, Korea A number of plant extracts and herbal extracts, such as Japanese Patent Application Laid-Open No. 96-033442) and Aloe (Japanese Patent Laid-Open No. 52-44375, Hei 2-207030, Hei 5-139950), act on tyrosinase to inhibit melanin production. Although it has been found that, they also have a lot of problems in the use of more than the effective concentration in cosmetics or pharmaceuticals in terms of safety, discoloration, etc., and still does not show an excellent effect.

Therefore, the present inventors have diligently researched to develop a whitening composition which is excellent in stability and less discoloration while suppressing melanin production. As a result, the extract of Delonix regia , a plant native to Taiwan and southern China, reduces melanin production. It was confirmed that it can be usefully used as a whitening composition by acting on the skin stably, and completed the present invention.

Therefore, it is an object of the present invention to provide a composition for whitening containing phoenix tree extract as an active ingredient.

Still another object of the present invention is to provide a cosmetic composition containing phoenix tree extract and a dermatologically acceptable carrier and a food composition containing phoenix tree extract and a foodologically acceptable carrier.

In one aspect, the present invention provides a composition for whitening containing phoenix tree extract as an active ingredient.

Delonix regia of the present invention is a flowering plant species of the family Fabaceae, which is distinguished by leaves and brilliant flowers such as fern plants, and has been cultivated as a decorative tree. It is widely distributed in the Caribbean, Hong Kong, Canary Islands, Taiwan, and southern China, and the seed pod of phoenix is used as a percussion instrument known as shak-shak or maraca in the Caribbean.

The phoenix tree extract according to the present invention is preferably extracted from the leaves.

More preferably, the extract may be prepared by hydrothermal extraction of a polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, most preferably by adding methanol.

The whitening effect of the extract is characterized in that it is made by inhibiting melanin production.

Looking at the manufacturing process of phoenix tree extract according to the present invention.

The phoenix is washed with water and dried in the shade. Dry phoenix tree is placed in an extraction container and extracted for a certain time at a suitable temperature in an appropriate amount of extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used, and preferably methanol is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of phoenix tree.

The extraction method may be an extraction method such as water bath extraction, reflux cooling extraction, ultrasonic extraction, etc., preferably may be extracted 1 to 5 times as an extraction method of water bath extraction.

The deposition temperature is 30 ~ 70 ℃, more preferably 40 ~ 60 ℃, most preferably maintained at about 50 ℃ is most effective in the prevention of decay, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

After the phoenix tree extract is obtained, the solids may be removed using a filter paper, the suspension may be centrifuged, and the supernatant may be filtered under reduced pressure.

The filtered extract may be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

As a result of testing the efficacy of the phoenix tree extract according to the present invention, by reducing the melanin production by melanin-producing cells has a whitening effect (Experimental Example 1-2), it was confirmed that less cytotoxicity (Experimental Example 2). Therefore, phoenix tree extract of the present invention can be usefully used as a composition for skin whitening because it can exhibit a whitening effect by inhibiting the production of melanin without causing toxicity to cells.

The composition according to the present invention can be used orally or parenterally, the method of applying to the skin during parenteral use can be used, and the preferred method of use can be selected according to the dosage form.

As another aspect, the phoenix tree extract of the present invention may be provided as a cosmetic composition by mixing with a dermatologically acceptable carrier. The phoenix tree extract in the composition of the present invention may be contained in an amount of 0.001 to 50% by weight based on the total weight of the composition.

Cosmetics prepared from the cosmetic composition containing phoenix tree extract of the present invention can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to the cosmetic containing the phoenix tree extract of the present invention, by containing a dermatologically acceptable medium or base may be prepared in the form of adjuvants that can be topically or systemically applied commonly used in the field of dermatology.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

“Dermatologically acceptable carriers” that can be used according to the desired formulation include purified water, oils, waxes, fatty acids, fatty alcohols, fatty acid esters, surfactants, humectants, thickening agents, antioxidants, and viscosity stabilizers. These include, but are not limited to, topical stabilizers, chelating agents, buffers, preservatives, lower alcohols, and the like, and the types and concentrations thereof are varied and those skilled in the art do not depart from the spirit and scope of the present invention. Includes parts that can be modified and changed. If necessary, a whitening agent, a moisturizer, an anti-inflammatory agent, an antibacterial agent, an antifungal agent, a vitamin, a sunscreen agent, an antibiotic, an anti-acne agent, a perfume, a dye may be included, and these are cosmetic compositions according to the present invention in amounts commonly used in the cosmetic field. Can be included. Suitable from 0.001 to 50% by weight per total weight of the composition.

Specifically, the oil may be hydrogenated vegetable oil, perm oil, cottonseed oil, olive oil, palm oil, jojoba oil, avocado oil, wax, beeswax, carnauba, candelilla, montan, ceresin, liquid paraffin Lanolin may be used. Stearic acid, linoleic acid, linolenic acid, oleic acid may be used as a fatty acid, cetyl alcohol, octyl dodecanol, oleyl alcohol, pantenol, lanolin alcohol, stearyl alcohol, hexadecanol may be used as a fatty acid. As the fatty acid ester, isopropyl myristate, isopropyl palmitate, butyl stearate and the like may be used, but is not limited thereto.

Examples of the surfactant include anionic surfactants such as sodium stearate, sodium cetyl sulfate, polyoxyethylene laurylether phosphate, sodium N-acyl glutamate; Cationic surfactants such as stearyldimethylbenzylammonium chloride and stearyltrimethylammonium chloride; Amphoteric surfactants such as alkylaminoethylglycine hydrochloride and lecithin; Glycerin monostearate, sorbitan monostearate, propylene glycol monostearate, polyoxyethylene oleyl ether, polyethylene glycol monostearate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene ethylene coconut fatty acid monoethanol arnide ( monoethaolarnide), polyoxypropylene glycol, polyoxyethylene castor oil, nonionic surfactants such as polyoxyethylene lanolin and the like.

Glycerin, 1,3-butylene glycol, propylene glycol may be used as the moisture absorbent, and ethanol or isopropanol may be used as the lower alcohol. Examples of thickeners include sodium alginate, sodium caseate, gelatin agar, xanthan gum, starch, cellulose ethers (e.g., hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose, hydroxy propylmethyl cellulose), polyvinyl pyrrolidone, Polyvinyl alcohol, polyethylene glycol, sodium carboxymethyl cellulose, and the like, but are not limited thereto. As antioxidants, butylated hydroxytoluene, butylated hydroxyanisole, propyl gallate, citric acid, ethoxyquin can be used, and chelating agents include disodium edetate and ethane hydroxy diphosphate. Citric acid, sodium citrate, boric acid, borax, disodium hydrogen phosphate are available as buffers, and methyl parahydroxybenzoate, ethyl parahydroxybenzoate, dihydro as preservatives. Acetic acid, salicylic acid, benzoic acid are available, but are not limited to these.

The composition of the present invention may contain one or more skin whitening active ingredients exhibiting the same or similar function in addition to the phoenix tree extract. Skin whitening active ingredients include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanones, methylenedioxyphenyl alkanol, 2,7-dinitroindazole, Plant extracts such as vine tangerine extract, rice extract, licorice extract, and the like, but are not limited thereto.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, cleansing agents, face washes, soaps, treatments, Essences and the like.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

As another aspect, the phoenix tree extract of the present invention may be provided as a food composition in admixture with a food acceptable carrier. When the phoenix tree extract of the present invention is used as a food or beverage additive, the phoenix tree extract may be added as it is or used together with other food or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the phoenix tree extract may be suitably determined according to the purpose of use (prevention, health or therapeutic treatment).

In the case of long-term intake for health and hygiene or health control, the phoenix tree extract can be taken for a long time because there is no problem in terms of safety.

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, dairy products including other noodles, gum, ice cream, various soups, drinks, tea, and drinks. Alcoholic beverages and vitamin complexes.

The liquid component added in addition to the phoenix tree extract when formulated into a beverage is not limited to the above, but may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in conventional beverages. Examples of the aforementioned natural carbohydrates include monosaccharides (e.g. glucose, fructose, etc.), disaccharides (e.g. maltose, sucrose, etc.) and polysaccharides (e.g. conventional sugars such as dextrins, cyclodextrins, etc.), and xylitol Sugar alcohols such as sorbitol and erythritol. The proportion of said natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention. As flavoring agents other than those mentioned above, natural flavoring agents (taumarin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (e.g., saccharin, aspartame, etc.) can be used. .

In another embodiment, the food composition of the present invention comprises various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic and natural flavors, colorants and enhancers (such as cheese, chocolate), pectic acid and salts thereof, organic acids , Protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The food composition of the present invention may also contain pulp for the production of fruit and vegetable drinks. These components may be used alone or in combination, and the ratio of such additives is generally selected in the range of 0.001 to 50% by weight based on the total weight of the composition.

The phoenix tree extract according to the present invention can be usefully used as a composition for skin whitening because it exhibits a whitening effect by inhibiting the production of melanin without causing toxicity to cells.

Hereinafter, the examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, those skilled in the art. Will be self-evident.

<Example: Preparation of phoenix tree extract for skin whitening

The phoenix tree of this example was taken from the alpine region of the Philippines.

50 g of the collected plant was immersed in 200 ml of 100% methanol and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Methanol was evaporated from the filtrate using nitrogen, concentrated and then dissolved in 10% methanol to prepare an extract.

Experimental Example 1: Measurement of the Whitening Effect

1-1: B16 Melanoma Cell Culture

B16 melanoma cells were provided by Suwon Forest Research Institute and cultured. B16 melanoma cells are cell lines producing melanin and are commonly used for whitening screening.

The medium for culturing the cells was sterile by adding DMEM, 10% FBS, 1% penicillin / streptomycin. Cell cultures were incubated in an incubator at 37 ° C., 5% CO ₂ . Melanoma cells were cultured and passaged when the 100 mm Petri dishes were about 90% full.

1-2: melanin inhibition measurement using absorbance measurement

In order to measure the whitening effect of phoenix tree extract, the amount of melanin produced by treatment with B16 melanoma cells was measured. Cells were seeded on 6-well plates and the extracts prepared in Example were treated at 100 µg / ml, 50 µg / ml and 25 µg / ml concentrations, and then cultured for 2 days to recover the cells. The recovered cells were added with 1N NaOH containing 10% DMSO, reacted at 90 ° C for 1 hour to be sufficiently dissolved in melanin, and then absorbance was measured at a wavelength of 405 nm using an ELISA reader.

Experimental results were calculated using the following equation 1 to calculate the melanin production, it is shown in Table 1. Arbutin, which is known as a general whitening substance, has 87% melanin at 200 μg / ml compared to untreated, whereas phoenix tree extract has higher melanin than arbutin at 86% at 100 μg / ml. It could be confirmed that it has an inhibitory effect.

Melanin Formation (%) = Treatment Absorbance / Untreated Absorbance × 100

Inhibition of Melanin Production by Phoenix Extract Extract concentration (µg / ml) Melanin Content (% Control) 100 86.67 ± 1.35 50 97.13 ± 0.55 25 99.73 ± 4.21

Experimental Example 2: Toxicity Test

In order to use it as a whitening cosmetic of phoenix tree extract, an experiment was performed to confirm the cytotoxicity. Cells were inoculated into 96 well plates and treated with 100 µg / ml, 50 µg / ml and 25 µg / ml, respectively, after 2 days, the medium was removed, and 100 µl of MTT solution was added. After the removal, 100 μl of DMSO was added to dissolve the stained cells, and the absorbance was measured at a wavelength of 540 nm using an ELISA reader.

Cell viability was calculated using Equation 2 below, and the results are shown in Table 2. Compared to the cells untreated with phoenix tree extract, despite the high concentration of 100 ㎍ / ㎖, it can be seen that there is no cytotoxicity with a viable cell rate of 95%.

Cell viability (%) = (1-treated absorbance / untreated absorbance) × 100

Cytotoxicity Assay of Phoenix Fruit Extract Extract concentration (µg / ml) Cytotoxicity (% control) 100 95.81 ± 1.86 50 105.30 ± 3.85 25 106.79 ± 3.36

Examples of formulations for the composition of the present invention are illustrated below.

<Example 1: Flexible lotion>

Formulation example of the flexible lotion containing phoenix tree extract was prepared as shown in Table 3.

Flexible lotion with phoenix tree extract Raw material Content (% by weight) Phoenix tree extract 10.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<Example 2: Nutritional Cream>

Formulation of nutrition cream containing phoenix tree extract was prepared as shown in Table 4.

Nourishing Cream with Phoenix Extract Raw material Content (% by weight) Phoenix tree extract 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium aluminum silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Quantity Triethanolamine Quantity Preservative Quantity Flavoring agent Quantity Purified water Balance

Preparation Example 3 Preparation of Food

Foods containing phoenix tree extract of the present invention was prepared as follows.

3-1. Manufacture of flour food products

0.5 to 5.0 parts by weight of phoenix tree extract of the present invention was added to flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare foods for health promotion.

3-2. Manufacture of dairy products

5-10 parts by weight of the phoenix tree extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

3-3. Manufacture of wire

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The phoenix tree extract of the present invention was decompressed and concentrated in a vacuum concentrator, and the dried product obtained by drying with a sprayer and a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

It was prepared by combining the dry powder of the grains, seeds and phoenix tree extract prepared in the following ratio.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

Dry powder of phoenix tree extract (3 parts by weight),

(0.5 part by weight),

Foxglove (0.5 part by weight)

Claims (5)

delete delete delete Cosmetic composition comprising phoenix tree extract and a dermatologically acceptable carrier. A food composition comprising phoenix tree extract and a food acceptable carrier.