KR20210134589A - Composition for improving skin comprising an extract of Pueraria thomsonii or a compound derived therefrom - Google Patents

Abstract

The present invention relates to a cosmetic composition, a food composition, or a quasi-drug composition comprising Pueraria thomsonii Benth or Pueraria thomsonii flower extracts, or a compound derived therefrom as an active ingredient. The composition of the present invention is highly effective in melanin reduction, anti-inflammatory activity, collagen synthesis promotion, and anti-glycosylation activity, thereby being effectively used for skin whitening, skin trouble alleviation, skin elasticity enhancement or wrinkle alleviation, antioxidant activity, and skin moisturizing. Therefore, the composition of the present invention can be used as a cosmetic composition, a food composition, and a quasi-drug composition, that are safe for the skin and highly effective in improving skin condition.

Description

Composition for improving skin comprising an extract of Pueraria thomsonii or a compound derived therefrom

The present invention relates to a pulverized or pulverized extract; Or a compound derived from the extract; relates to a cosmetic composition, food composition or quasi-drug composition comprising as an active ingredient.

Specifically, for skin whitening containing the substance as an active ingredient; for improving skin troubles; For improving skin elasticity or improving wrinkles; for antioxidant; Or it relates to a composition for moisturizing the skin.

Human skin color is determined by several factors such as melanocyte activity, blood vessel distribution, skin thickness, and the presence or absence of pigments inside and outside the human body. In particular, enzymes such as tyrosinase are The black pigment called melanin, which is produced by action, is important. Melanin exists in the skin and has an important function of protecting the body from ultraviolet rays, but when it is overproduced, it is known that it promotes pigmentation and skin aging and plays a major role in inducing skin cancer.

In order to treat or reduce excessive melanin pigmentation, ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, substances with tyrosinase inhibitory activity from before These were used in combination with cosmetics or pharmaceuticals. However, their use is limited due to insufficient whitening effect, safety problem for skin, stability problem when formulated in cosmetics, and the like.

In addition, factors that threaten skin health such as an increase in the amount of ultraviolet rays due to environmental pollution, a westernized lifestyle, and stress are increasing recently. These cause skin contamination, dryness, troubles, and abnormal skin immune system, and eventually cause dermatitis, roughness, rapid aging, and the like.

In particular, there is a problem in that the concentration of reactive oxygen species in the body is increased, and these reactive oxygen species attack lipids, proteins, sugars, DNA, etc., which are cellular components, and cause a peroxidation reaction, thereby promoting skin aging. Accordingly, there is a need for development of a cosmetic composition having an antioxidant effect that inhibits the generation of reactive oxygen species and an anti-aging (skin aging prevention) effect through this.

On the other hand, cosmetics are used to protect the skin, and various ingredients such as surfactants, preservatives, fragrances, sunscreens, and pigments are known to cause various troubles such as inflammation, pimples, and edema on the skin (Maibach. HI, Contact Dermatitis). , 6. 369-404, 1980). In addition, it is known that skin inflammation is also caused by ultraviolet rays from the sun, and sebum, sweat, fatty acids in cosmetics, proteins, etc. discharged from the body are decomposed into highly toxic substances by the skin flora present on the skin. It may cause inflammation.

Inflammatory reaction is a physiological reaction to protect the living body from invasion of foreign substances such as bacteria and mechanical damage. It can also cause harmful damage to fields. Therefore, if the inflammatory factors are not eliminated, chronic inflammation occurs as a result, resulting in more serious tissue damage. Accordingly, there is an urgent need to develop a cosmetic composition capable of initially suppressing skin troubles through an anti-inflammatory effect.

Collagen is a major matrix protein produced in fibroblasts of the skin, and functions such as maintaining the mechanical firmness of the skin and tissue bonding, supporting cell adhesion, and inducing cell differentiation. Collagen decreases due to age increase and photoaging by UV irradiation, and collagen reduction is promoted by the activity of collagenase enzyme that decomposes collagen. This is known to be closely related to the formation of wrinkles on the skin. In addition, collagen and elastin fibers play an important role in retaining moisture in the dermal layer, and are known to be involved in maintaining skin elasticity through skin moisturizing.

Currently, retinol, adenosine, chlorella extract and the like are known as skin elasticity enhancement and wrinkle improvement cosmetics. Retinol promotes collagen synthesis and inhibits the elastase enzyme, but it is unstable and has limited usage due to safety issues such as irritation and rash when applied to the skin. It is known that it is difficult to expect a wrinkle improvement effect.

In addition, in the body, a non-enzymatic reaction in which a simple sugar such as glucose or fructose forms a covalent bond with a protein or fat of a protein occurs, which is called glycation. Glycosylated collagen prevents the collagen from forming an appropriate structure in the extracellular matrix of the dermal layer, thereby causing the skin to lose its elasticity and promote wrinkle formation. In addition, advanced glycation endproducts (AGEs) generated by glycation are brownish substances, and as skin aging progresses, they accumulate on the upper dermis layer and the reflected light reflected from the skin is reduced. Therefore, the amount of final glycation products increases as skin aging progresses, and it is considered as one of the substances that gradually make the face look yellow and dull (Hiroshi, O. et al, Skin Research and Technology, 15, p. 496, 2009). .

Aminoguanidine, Pyridoxamine, Aspirin, etc. are known as substances that inhibit glycation. However, these substances have a limited amount of use due to safety concerns on the skin or are practically effective due to insignificant effects. There is a problem that cannot be expected.

Under the above background, the present inventors have conducted research on substances having excellent skin whitening, skin trouble improvement, skin elasticity enhancement or wrinkle improvement, antioxidant, and skin moisturizing effects among natural resources existing in nature, powder or powder The present invention was completed by confirming that a composition comprising a brown algae extract, or a compound derived therefrom, has the effect of reducing melanin, anti-inflammatory, promoting collagen synthesis, and anti-glycosylation.

An object of the present invention is a pulverized or pulverized extract; Or a compound derived from the extract; to provide a cosmetic composition comprising as an active ingredient.

Specifically, for skin whitening containing the substance as an active ingredient; for improving skin troubles; For improving skin elasticity or improving wrinkles; for antioxidant; Or to provide a cosmetic composition for moisturizing the skin.

Another object of the present invention is a pulverized or pulverized extract; Or a compound derived from the extract; to provide a food composition comprising as an active ingredient.

Another object of the present invention is a pulverized or pulverized extract; Or a compound derived from the extract; to provide a quasi-drug composition comprising as an active ingredient.

One aspect of the present invention is a pulverized or pulverized extract; Or a compound derived from the extract; provides a cosmetic composition comprising as an active ingredient.

In the present invention, "bungal (粉葛)" refers to Gamgaldeung ( Pueraria thomsonii,甘葛藤), specifically, the tuberous root of Gamgaldeung. It is cylindrical, quadrangular, or semi-cylindrical, and has a length of 12-15 cm and a diameter of 4-8 cm. The outer surface is yellowish white or light brown, and the uncoated ones are grayish brown, and the cross section is white. It is heavy and hard, and the cut side is powdery, and the longitudinal cross section has several vertical stripes made of fibers. In oriental medicine, it is used to treat indigestion, headache, anemia, dysentery, abdominal pain, alcohol poisoning, cold, vomiting and bleeding.

In the present invention, "Bungalhwa (粉葛花)" refers to a flower part of a branch, specifically, a flower bud or a flower just starting to bloom. In oriental medicine, it has been mainly used to treat bleeding, and has a unique sweet taste that relieves alcohol poisoning and strengthens the intestines.

In the present invention, pulverization or pulverization may be purchased and used commercially sold, or harvested or cultivated in nature may be used.

In the present invention, "extract" is an extract obtained by pulverization or pulverization extraction treatment, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, etc. It includes extracts of all formulations that can be formed using the extract itself and the extract. The extract may be extracted from pulverized or pricked natural, hybrid or varietal plants, and may also be extracted from plant tissue cultures.

The method of extracting the pulverized or pulverized extract in the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. It includes hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, and the like, and may be performed alone or in combination of two or more methods, but is not limited thereto.

The type of extraction solvent used for extraction in the present invention is not particularly limited, and any solvent known in the art may be used. As the extraction solvent, water (or distilled water); lower alcohols having 1 to 4 carbon atoms, such as methanol, ethanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, but is not limited thereto. In addition, a solvent extract may be prepared by extracting pulverization or pulverization one or more times using the solvent, and a dry extract obtained by distilling the solvent extract under reduced pressure and then freeze-drying or spray-drying may be prepared.

In an embodiment of the present invention, the skin improvement effect was confirmed after preparing a powdered powder and a powdered extract using water (or distilled water).

In the present invention, the pulverized or pulverized extract may be included in the form of a fraction thereof, a processed product thereof, animals/plants containing the same, and an extract thereof.

In the present invention, "fraction" refers to a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture containing various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. Non-limiting examples of the fractionation method include, but are not limited to, a method of obtaining a fraction from the extract by treating an extract obtained by extracting pulverization or pulverization with a predetermined solvent.

The kind of solvent used for the fraction is not particularly limited, and any solvent known in the art may be used. Examples of the fractionation solvent include polar solvents such as water and alcohol; A non-polar solvent such as hexane, ethyl acetate, chloroform, and dichloromethane may be used, and these may be used alone or in combination of two or more, but is not limited thereto.

Specifically, in the present invention, the compound derived from the pulverized or pulverized extract may be one of the compounds represented by the following Chemical Formulas 1 to 3.

[Formula 1]

[Formula 2]

[Formula 3]

The compound represented by Formula 1 is tectorigenin 7-O-xylosylglucoside, a compound having a molecular _{formula of C 27} H ₃₀ O _{15 and a molecular weight of 594.5.} The compound represented by Formula 2 is tectoridin, a compound having a molecular formula of C ₂₂ H ₂₂ O ₁₁ and a molecular weight of 462.4. The compound represented by Formula 3 is tectorigenin, a compound having a molecular _{formula of C 16} H ₁₂ O _{6 and a molecular weight of 300.26.}

It is known that the three kinds of compounds are a kind of isoflavonoid-based compounds, and can be obtained from pulverized or pulverized extracts.

In the present invention, the route of obtaining the compound is not particularly limited, and it can be chemically synthesized by a method known in the art, separated from a pulverized or pulverized extract, or other kinds of natural products, or commercially available materials can be purchased and used. have.

Specifically, the cosmetic composition of the present invention is for skin whitening; for improving skin troubles; For improving skin elasticity or improving wrinkles; for antioxidant; Or it may be for skin moisturizing.

In the present invention, "skin whitening" means to lighten skin tone by inhibiting the synthesis of melanin pigment, and to improve skin hyperpigmentation such as spots or freckles caused by ultraviolet rays, hormones, or heredity. Such a whitening effect may be achieved, for example, by reducing the total amount of melanin pigment, but the present invention is not limited to this mechanism of action.

In the present invention, "improving skin troubles" means inhibiting or inhibiting the generation of troubles on the skin, or alleviating troubles that have already been generated, and can be achieved by anti-inflammatory, but the present invention is based on this mechanism of action. It is not limited.

The skin trouble refers to a skin disease that may be caused by inflammation caused by excessive nitric oxide production in macrophages. All skin diseases related to inflammation are included regardless of the type, and the skin diseases related to inflammation include atopic dermatitis, psoriasis, erythematous disease triggered by radiation, chemicals, burns, acid burns, bullous dermatosis, lichenoid form. type disease, allergic itch, seborrheic eczema, rose acne, pemphigus vulgaris, erythema multiforme, erythema nodosum, balanitis, vulvitis, inflammatory hair loss such as alopecia areata, skin T-cell lymphoma, etc. , skin rash, acne, pimples, rosacea (red nose), but is not limited thereto.

The anti-inflammatory means suppressing inflammation caused in the skin, which can be achieved by suppressing excessive nitric oxide (NO) production. In particular, the composition of the present invention may have an effect of improving skin troubles such as acne through an anti-inflammatory effect.

In the present invention, "promoting skin elasticity" means It means to relieve the degree of sagging or sagging skin. In addition, "wrinkle improvement" means suppressing or inhibiting the generation of wrinkles on the skin, or alleviating the wrinkles already formed.

In the present invention, "antioxidation" means inhibiting cellular oxidation by highly reactive free radicals or reactive oxygen species depending on intracellular metabolism or oxidative stress caused by the influence of ultraviolet rays. and removing free radicals or reactive oxygen species, thereby reducing cell damage.

In the present invention, "moisturizing the skin" means to increase the feeling of moisture in the skin and to maintain a moist state. If you increase the skin moisturizing effect, it can have a beneficial effect on improving wrinkles and increasing elasticity of the skin.

Specifically, the composition of the present invention may have a melanin reduction effect, an anti-inflammatory effect, a collagen synthesis promoting effect, and an anti-glycosylation effect. In an embodiment of the present invention, the powdered extract, the powdered extract, and the compounds represented by Formulas 1 to 3, respectively, inhibit melanin production, inhibit NO production, and promote the synthesis of type I collagen, , there is an antioxidant effect and anti-glycosylation effect (Tables 1 to 5), the composition of the present invention can be usefully used for skin whitening, skin trouble improvement, skin elasticity enhancement or wrinkle improvement, antioxidant and / or skin moisturizing purpose Confirmed.

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutrient lotion, softening lotion, pack, soft water, emulsion, makeup base, essence, soap, liquid detergent, bath agent, sunscreen cream, sun oil, suspension, emulsion It can be prepared in a formulation selected from the group consisting of liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray, but is not limited thereto. .

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and conventional ingredients include, for example, oil, water, surfactant, humectant, lower alcohol, Thickeners, chelating agents, colorants, preservatives, fragrances and the like may be appropriately mixed, but the present invention is not limited thereto. The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures thereof may be used, but are not limited thereto.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof may be used as a carrier component. In particular, in the case of a spray, additional chlorophyll propellants such as, but not limited to, fluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol fatty ester, fatty acid ester of polyethylene glycol or sorbitan may be used. However, it is not limited thereto.

When the formulation of the present invention is a suspension, as a carrier component a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugar, etc. may be used as carrier components. can, but is not limited thereto.

Another aspect of the present invention is a pulverized or pulverized extract; Or a compound derived from the extract; provides a food composition comprising as an active ingredient.

Specifically, in the present invention, the compound derived from the pulverized or pulverized extract may be one of the compounds represented by the following Chemical Formulas 1 to 3.

[Formula 1]

[Formula 2]

[Formula 3]

Specifically, the food composition of the present invention is for skin whitening; for improving skin troubles; For improving skin elasticity or improving wrinkles; for antioxidant; Or it may be for skin moisturizing.

Powdering, powdering, Formulas 1 to 3, skin whitening, skin trouble improvement, skin elasticity enhancement or wrinkle improvement, antioxidant, and skin moisturizing are the same as described above.

The food composition may be used in the form of health functional food, but is not limited thereto. In addition, the food composition of the present invention is a powdered or powdered extract; or a compound derived from said extract; In addition, it may include food supplementary additives that are acceptable for food.

In the present invention, "food supplement additive" means a component that can be added auxiliary to food, it is added to the manufacture of health functional food of each formulation, and can be appropriately selected and used by those skilled in the art. Examples of food supplement additives include various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners , pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, and the like.

The food composition of the present invention may include a health functional food. The health food refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and pills using raw materials or ingredients useful for the human body. Here, the term "functionality" refers to obtaining useful effects for health purposes, such as regulating nutrients or physiological effects with respect to the structure and function of the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and at the time of manufacture, it can be prepared by adding raw materials and components commonly added in the art. In addition, if the formulation of the health functional food is also recognized as a health functional food, it may be manufactured without limitation. The food composition of the present invention can be prepared in various forms, and unlike general drugs, it has the advantage that there are no side effects that may occur during long-term administration of the drug using food as a raw material, and has excellent portability, Health functional food can be taken as a supplement for skin whitening, skin trouble improvement, skin elasticity improvement or wrinkle improvement, antioxidant, and skin moisturizing effect.

There is no limitation in the form that the health functional food of the present invention can take, and it may include any food in a conventional sense, and may be used interchangeably with terms known in the art, such as functional food. In addition, the health functional food of the present invention can be prepared by mixing known additives with other suitable auxiliary ingredients that may be included in the food according to the selection of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and There are vitamin complexes and the like, and it can be prepared by adding the extract according to the present invention as a main component to juice, tea, jelly, juice, and the like. It also includes food used as feed for animals.

Another aspect of the present invention is a powdered or crushed extract; Or a compound derived from the extract or a pharmaceutically acceptable salt thereof; provides a quasi-drug composition comprising as an active ingredient.

Specifically, in the present invention, the compound derived from the pulverized or pulverized extract may be one of the compounds represented by the following Chemical Formulas 1 to 3.

[Formula 1]

[Formula 2]

[Formula 3]

Specifically, the quasi-drug composition of the present invention is for skin whitening; for improving skin troubles; For improving skin elasticity or improving wrinkles; for antioxidant; Or it may be for skin moisturizing.

Powdering, powdering, Formulas 1 to 3, skin whitening, skin trouble improvement, skin elasticity enhancement or wrinkle improvement, antioxidant, and skin moisturizing are the same as described above.

The quasi-drug composition of the present invention includes powdered or powdered extract; or a compound derived from said extract; In addition, if necessary, it may further include a pharmaceutically acceptable carrier, excipient or diluent. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as it does not impair the effects of the present invention, and includes, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, preservatives, etc. may include

Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, acacia gum, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , injectable esters, Witepsol, Macrogol, Tween 61, cacao butter, laurage, and the like, but are not limited thereto.

The quasi-drug composition of the present invention may include, but is not limited to, disinfectant cleaner, shower foam, ointment, wet tissue, coating agent, etc. It may be appropriately selected from the techniques of

In addition, the compounds represented by Chemical Formulas 1 to 3 included in the composition of the present invention may be included in the form of a cosmetically, food, or pharmaceutically acceptable salt. This refers to a salt in a form that can be used cosmetically, food or pharmaceutical among salts, which are substances in which cations and anions are combined by electrostatic attraction. Any and all organic or inorganic addition salts in which the side effects attributable to the salt do not diminish the beneficial efficacy of the compound. Such salts include acid addition salts formed with free acids or metal salts formed with bases.

Organic acids and inorganic acids can be used as free acids, and organic acids include methanesulfonic acid, p-toluenesulfonic acid, acetic acid, trifluoroacetic acid, maleic acid, succinic acid, oxalic acid, benzoic acid, tartaric acid, and fumaric acid. , manderic acid, propionic acid, citric acid, lactic acid, glycolic acid, gluconic acid, galacturonic acid, glutamic acid, glutaric acid , glucuronic acid, aspartic acid, ascorbic acid, carbonic acid, vanillic acid, hydroiodic acid, etc. can be used, and as the inorganic acid, hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, tartaric acid, etc. can be used. , but not limited thereto.

In addition, a base may be used to prepare a cosmetically or pharmaceutically acceptable metal salt. The alkali metal salt or alkaline earth metal salt is obtained, for example, by dissolving the compound in an excess alkali metal hydroxide or alkaline earth metal hydroxide solution, filtering the undissolved compound salt, and then evaporating and drying the filtrate. In this case, as the metal salt, it is cosmetically suitable to prepare a sodium, potassium, or calcium salt in particular, but is not limited thereto. Also, the corresponding silver salt can be obtained by reacting an alkali metal or alkaline earth metal salt with a suitable silver salt (eg, silver nitrate).

The compounds represented by Formulas 1 to 3 or a salt thereof of the present invention may exist in unsolvated as well as solvated forms including hydrates and ethanolates. In addition, the compound represented by Formulas 1 to 3 or a salt thereof of the present invention may exist in a crystalline or amorphous form, and all such physical forms are included in the scope of the present invention.

The cosmetic composition, food composition, and quasi-drug composition of the present invention may additionally contain one or more active ingredients exhibiting the same or similar functions. For example, it may include skin whitening, skin trouble improvement, skin elasticity enhancement or wrinkle improvement, antioxidant and/or skin moisturizing ingredients known in the art to which the present invention pertains. Due to the additional ingredients, the skin whitening, skin trouble improvement, skin elasticity enhancement or wrinkle improvement, antioxidant and/or skin moisturizing effect of the composition of the present invention may be further enhanced. The content range of the additional component may be appropriately adjusted by those skilled in the art in consideration of skin safety, ease of formulation, and stability of active ingredients according to combined use.

Furthermore, the present invention relates to a pulverized or pulverized extract; or a compound derived from the extract; skin whitening, including applying to the skin of an individual; improvement of skin troubles; increase skin elasticity or improve wrinkles; antioxidant; or a method of moisturizing the skin. The subject may include, without limitation, mammals including rats, livestock, and humans.

The composition of the present invention has excellent melanin reduction, anti-inflammatory, collagen synthesis promotion, and anti-glycosylation effects, and thus can be usefully used for skin whitening, skin trouble improvement, skin elasticity enhancement or wrinkle improvement, antioxidant, and skin moisturizing. Therefore, the composition of the present invention can be used as a cosmetic composition, a food composition, and a quasi-drug composition that is safe for the skin and has an excellent skin condition improvement effect.

Hereinafter, the present invention will be described in more detail through examples. These examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of powdered extract

After the powder was dried well and chopped, 100 g of dry weight was placed in a flask, and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare a powdered extract.

Example 2: Preparation of pulverized extract

After the flowers and buds of Bungal were well dried and chopped, 100 g of dry weight was placed in a flask, and extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare a pulverized extract.

Example 3: Preparation of compounds

The route of obtaining compounds 1 to 3 is not particularly limited, and may be chemically synthesized by a method known in the art or used in an experiment using commercially available materials.

Experimental Example 1: Whitening effect according to the inhibition of melanin production

Examples 1 and 2, compounds 1 to 3 were added to the culture medium of B-16 mouse melanoma cells, and the whitening effect was confirmed by measuring the total amount of melanin at the cell level (Lotan R., Lotan D. Cancer Res. 40:3345-3350, 1980). Before the experiment, toxicity was evaluated for melanoma cells in mice, and a non-toxic concentration was selected for whitening evaluation.

The final concentration of the culture medium was 10 ppm or 100 ppm, and arbutin, a control, was added to the medium to 100 ppm, treated with B-16 melanoma cells, respectively, and cultured for 3 days. Thereafter, the cells were treated with trypsin, removed from the culture vessel, centrifuged, and then melanin was extracted. To the detached cells, 1 mL of sodium hydroxide solution (1N concentration) was added and boiled for 10 minutes to dissolve melanin, and then the amount of generated melanin was measured by measuring the absorbance at 400 nm using a spectrophotometer.

The amount of melanin was measured by a method indicating the absorbance of the unit cell number (1×10 ⁶ cells), the total amount of melanin relative to the control was calculated as the inhibition rate (%), and the results are summarized in Table 1.

sample Melanin total inhibition rate (%) control - Positive control (arbutin, 100ppm) 47.76 Example 1 (100 ppm) 50.41 Example 2 (100 ppm) 52.78 Compound 1 (10 ppm) 48.31 Compound 2 (10 ppm) 63.08 Compound 3 (10 ppm) 32.72

As can be seen from the results in Table 1, the powdered extract (Example 1), the powdered extract (Example 2), and compounds 1 to 3 all showed excellent melanin reduction effects even at low concentrations, indicating that they could be used for skin whitening purposes. Confirmed.

Experimental Example 2: Anti-inflammatory effect according to inhibition of NO production

In order to confirm the anti-inflammatory effect and skin trouble improvement effect of Examples 1 and 2 and Compounds 1 to 3, nitric oxide (nitric oxid: NO) formation inhibition test was conducted.

Specifically, RAW264.7 cells, which are macrophages of mice, were subcultured several times, ^{and then put into a 24-well plate 3×10 5} each, and cultured for 24 hours. Then, it was replaced with a cell medium diluted to a final concentration of 10 or 100 ppm. At this time, the NO production inhibitory substance L-NMMA (L-NG-monomethylarginine) was treated together as a positive control and incubated for 30 minutes, and 1 μg of LPS (lipopolysaccharide) was treated as a stimulus and cultured for 24 hours. 100 μl of the supernatant was taken and transferred to a 96-well plate, 100 μL of GRIESS solution was added and reacted at room temperature for 10 minutes. The NO production inhibition rate (%) was calculated by measuring the absorbance at 540 nm, and the results are shown in Table 2 below.

sample NO production inhibition rate (%) Control (DMSO, 10 ppm) - Positive control (L-NMMA, 10ppm) 30.58 Example 1 (100 ppm) 24.41 Example 2 (100 ppm) 26.86 Compound 1 (100 ppm) 99.51 Compound 2 (100 ppm) 99.52 Compound 3 (10 ppm) 51.94

As can be seen from the results of Table 2, it was confirmed that the powdered extract (Example 1), the powdered extract (Example 2), and compounds 1 to 3 all showed excellent NO production inhibitory effects, so that they could be used for improving skin troubles. did. In addition, it can be expected to exhibit a synergistic effect when used together with other anti-inflammatory substances.

Experimental Example 3: Effect of promoting the synthesis of type I collagen in human-derived fibroblasts

Examples 1 and 2, and compounds 1 to 3 were added to the culture medium of human-derived fibroblasts to confirm the effect of promoting the synthesis of type 1 collagen at the cellular level. The measurement of synthesized collagen was quantified using the PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay KIT). In order to measure the amount of collagen synthesis, Examples 1 and 2, and compounds 1 to 3 were added to the fibroblast culture medium (DMEM medium) and cultured for 48 hours. The degree of collagen synthesis was measured at 450 nm using a spectrophotometer. For comparison of the effect, the collagen synthesis level was measured in the same way for the sample in which the untreated fibroblast culture medium (negative control group) and vitamin C (positive control group) were added to a final concentration of 52.85 μg/mL. The collagen production increase rate (%) was calculated as the ratio of the relative collagen production to the control, and the results are shown in Table 3 below.

sample Type 1 collagen production (ng/ml) Collagen production increase rate (%) negative control 150.2 - Positive control group (vitamin C, 50ppm) 210.9 40.4 Example 1 (100 ppm) 179.6 19.57 Example 2 (100 ppm) 187.4 24.77 Compound 1 (10 ppm) 202.3 34.7 Compound 2 (10 ppm) 221.9 47.7 Compound 3 (10 ppm) 208.1 38.5

As can be seen from the results in Table 3, the powdered extract (Example 1), the powdered extract (Example 2), and compounds 1 to 3 all promoted collagen synthesis, and used to enhance skin elasticity or improve wrinkles, and for skin moisturizing found that it can be used as In particular, it was confirmed that compounds 1 to 3 exhibited similar or superior collagen synthesis effects despite being used at a lower concentration than the positive control group.

Experimental Example 4: Antioxidant effect according to free radical scavenging

In order to confirm the antioxidant activity of Examples 1 and 2, and Compounds 1 to 3, free radical scavenging activity was measured. Free radical scavenging activity was measured using DPPH. DPPH was purchased from Sigma Co., Ltd. (USA) and used. First, a standard DPPH ethanol solution of 1.5 mM (0.06 mg/mL) was prepared. Then, make a 1000 μg/mL vitamin C solution using tertiary distilled water, and then serially dilute by 1/2 to 1000, 500, 250, 125, 62.5, 31.25, 15.625, 7.8125, 3.90625, 1.953125, 0.9765625, 0.48828125 μg A sample of vitamin C at a concentration of /mL was prepared. In Examples 1 and 2, dilution solutions of the compounds were prepared from 1000000 μg/mL concentrations, and in Formulas 1 to 3, at the same concentration as vitamin C. Then, the sample and the standard DPPH solution were added in the same ratio, stirred well, reacted at 37° C. for 30 minutes, and absorbance was measured at 520 nm. _{The free radical scavenging ability was obtained by obtaining IC 50} , which is the concentration of vitamin C and the compound of Formula 1 required to remove 50% of free radicals of the non-additive control group, and the results are shown in Table 5 below.

sample IC ₅₀ (%w/v) Positive control group (vitamin C) 0.0005 Example 1 0.0040 Example 2 0.0042 compound 1 0.0015 compound 2 0.0024 compound 3 0.0012

As can be seen from the results of Table 4, it was confirmed that the powdered extract (Example 1) and the powdered extract (Example 2), and compounds 1 to 3 all exhibit excellent free radical scavenging activity, and thus can be used for antioxidant purposes. In addition, it can be expected that it will exhibit a synergistic effect together with other substances in skin whitening, skin elasticity enhancement or wrinkle improvement, and skin moisturizing use.

Experimental Example 5: Anti-glycation effect

In order to confirm the glycation inhibitory effect, the glycation inhibitory activity was measured using L-arginine and glucose.

First, 1M L-arginine and 1M glucose were dissolved in 1M phosphate buffer (pH 7.4) and prepared by diluting the sample to 50 ppm and 100 ppm using 1M phosphate buffer. 1M L-arginine and 1M phosphate buffer were mixed in a ratio of 1:4, and then 80 μl of each was dispensed to a 96-well plate. Here, 100 μL of a sample diluted to 10 ppm and 100 ppm, respectively, and 0.01 M aminoguanidin to be used as a positive control were added. After mixing these samples well, finally, glucose diluted with 1M phosphate buffer was added so that the final concentration of glucose was 0.1M. The reaction was carried out at 70° C. for 4 hours. The degree of glycosylation was measured by measuring the absorbance at 420 nm of the 96-well plate using a spectrophotometer.

The glycation experimental group of the following formula was an experimental group in which glycation was induced by adding 1M L-arginine and 1M glucose, and in order to measure the absorbance of the sample itself, only 1M L-arginine and the sample were added and the absorbance was measured at 420 nm without adding glucose. The glycation inhibition rate (%) was calculated by the following formula, and the results are shown in Table 5.

sample Glycosylation inhibition rate (%) Control (DMSO, 50 ppm) - Positive control (Aminoguanidine, 55ppm) 48.12 Example 1 (100 ppm) 41.28 Example 2 (100 ppm) 40.08 Compound 1 (10 ppm) 41.67 Compound 2 (10 ppm) 38.95 Compound 3 (10 ppm) 34.75

As can be seen from the results of Table 5 above, it was confirmed that the powdered extract (Example 1) and the powdered extract (Example 2), and compounds 1 to 3 all exhibit excellent anti-glycosylation effects. In addition, it can be expected that it will exhibit a synergistic effect together with other substances in skin whitening, skin elasticity enhancement or wrinkle improvement, and skin moisturizing use.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims described below and their equivalents.

Claims (9)

A cosmetic composition for skin whitening comprising a flower extract of Pueraria thomsonii as an active ingredient. A cosmetic composition for improving skin troubles comprising a flower extract of Pueraria thomsonii as an active ingredient. A cosmetic composition for enhancing skin elasticity or improving wrinkles, comprising a flower extract of Pueraria thomsonii as an active ingredient. A cosmetic composition for antioxidants comprising a flower extract of Pueraria thomsonii as an active ingredient. A cosmetic composition for moisturizing the skin comprising a flower extract of Pueraria thomsonii as an active ingredient. A cosmetic composition for anti-saccharification comprising a flower extract of Pueraria thomsonii as an active ingredient. The composition according to any one of claims 1 to 6, wherein the composition is a solution, an external ointment, a cream, a foam, a nourishing lotion, a softening lotion, a pack, a softening water, an emulsion, a makeup base, an essence, a soap, a liquid detergent, a bathing agent , sunscreen cream, sun oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray Having a formulation, a cosmetic composition. For skin whitening comprising a flower extract of Pueraria thomsonii as an active ingredient; for improving skin troubles; For improving skin elasticity or improving wrinkles; for antioxidant; for moisturizing the skin; Or for anti-glycosylation, a food composition. For skin whitening comprising a flower extract of Pueraria thomsonii as an active ingredient; for improving skin troubles; For improving skin elasticity or improving wrinkles; for antioxidant; for moisturizing the skin; Or for anti-glycosylation, quasi-drug composition.