JP2011213600A - Bleaching agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new bleaching agent containing an extract of a flower of Pueraria lobata.SOLUTION: The bleaching agent and the cosmetic for bleaching contain the extract of the flower of the Pueraria lobata. The extract of the flower of the Pueraria lobata has tyrosinase activity-inhibiting activities and melanin formation-inhibiting activities, and the cosmetic containing the extract becomes the cosmetic for the bleaching, exhibiting excellent bleaching effects. The extract can be added to a food. The extract of the Pueraria lobata includes the extracts obtained by subjecting the flower of the Pueraria lobata, a pulverized product of the flower of the Pueraria lobata, a dried product of the flower of the Pueraria lobata and the powder of the flower of the Pueraria lobata to extraction treatment. The shapes of the extract of the flower of the Pueraria lobata can be any of liquid, paste and powder (sometimes, a case of powder of the essence of the flower of the Pueraria lobata is present) irrespective of the shape of the extract of the flower of the Pueraria lobata. Squeezed juice of the flower of the Pueraria lobata, the extract of the flower of the Pueraria lobata and the dried powder thereof (squeezed juice powder, extracted extract powder or the like) are preferable.

Description

  The present invention relates to a whitening agent characterized by containing a kuzu flower extract.

The skin color is mainly determined by the type and amount of coloring components such as melanin in the epidermis and dermis, but is controlled by various external and internal factors. If melanin is excessively produced by these factors, it causes local pigmentation, spots, freckles and the like, which is a major cosmetic problem. This melanin production is thought to be due to melanocytes. That is, melanocytes are activated by ultraviolet light, hormone secretion, and stimulating factors released from surrounding keratinocytes, and melanin production is promoted.

In addition, tyrosinase is activated by ultraviolet rays, lack of sleep, fatigue, etc., and tyrosinase turns tyrosine, an essential amino acid, into dopaquinone, and is thought to promote the synthesis of melanin by enzymatic or non-enzymatic oxidation of dopaquinone. .

Therefore, for whitening agents that solve cosmetic problems such as spots and buckwheat, it is important to “suppress the production of melanin” and “inhibit tyrosinase activity”. Under such circumstances, various new materials as whitening agents have been developed. (For example, Patent Documents 1 to 4).

JP 2008-50292 A JP 2005-289880 A JP 2005-120051 A Japanese Patent No. 3747053 WO2006 / 038721

Here, in the background of the diversification of whitening agents, whitening agents containing new materials that realize “inhibition of melanin production” and “inhibition of tyrosinase activity” in addition to materials conventionally known as whitening agents The offer of was desired.

In response to this expectation, the present invention provides a whitening agent containing a new material that realizes “suppression of melanin production” and “inhibition of tyrosinase activity”. That is, this invention is a whitening agent characterized by containing the Kuzuka extract. Further, the present invention is preferably a whitening agent, wherein the Kuzuka extract has a melanin production inhibitory action and a tyrosinase activity inhibitory action. Furthermore, it is a cosmetic for whitening characterized by containing the said whitening agent.

  ADVANTAGE OF THE INVENTION According to this invention, the whitening agent containing the new raw material which has a melanin production inhibitory effect and a tyrosinase activity inhibitory effect, ie, the whitening agent containing the kuzu flower extract, can be obtained. In addition, it does not specifically limit regarding the form of this invention, Kuzuka extract can be used as cosmetics, foodstuffs, etc. as it is or with various components added.

  Hereinafter, embodiments of the present invention will be described. The present invention should not be construed as being limited to the following embodiments, and various modifications can be made within the scope of the claims.

  The kuzu flower used in the present invention is a flower portion of the kuzu, which is a leguminous plant, and may include pistils, stamens, sepals, flower axes, bud leaves, buds and the like in addition to petals. The production area of Kuzu is not particularly limited.

  The kuzu flower extract used in the present invention includes an extract obtained by performing an extraction process from kuzu flower, kuzu flower crushed product, kuzu flower dry product, or kuzu flower powder. The shape of the kuzuhana extract is not limited and may be any of liquid, paste, and powder (sometimes referred to as kuzuka extract powder). Preferably, it is squeezed kuzuka juice, kuzuka extract, or a dry powder thereof (squeezed powder, extract extract powder, etc.). Kuzuka used in the present invention contains flavonoids such as isoflavones, saponins, tryptophan glycosides and the like, and preferably may contain 5% by weight or more of isoflavones. Among the kuzu flower extracts, the kuzu flower processed product from which insoluble solids (residues) have been removed in advance, such as the kuzu flower juice or the dried powder of the extract, It is suitably used as a product. Further, “Kuzu no Hana Extract” manufactured by Toyo Shinyaku Co., Ltd. can be preferably used.

  The whitening agent of the present invention can be used as it is or as a cosmetic or food with various commonly used components added. If necessary, raw materials and additives that are commonly used by those skilled in the art can be added.

  The whitening agent used in the present invention as cosmetics can be processed into various forms such as lotions, emulsions, gels, creams, ointments and the like. It is also used as cosmetics, pharmaceuticals, quasi drugs and the like. Specifically, it can be used as lotion, cosmetic cream, milky lotion, cream, pack, hair tonic, hair cream, shampoo, hair rinse, treatment, body shampoo, facial cleanser, soap, foundation, hair restorer, aqueous ointment, spray, etc. (Patent Document 5).

The whitening agent used in the present invention as a food can be used by mixing with various components (for example, excipients, extenders, binders, thickeners, emulsifiers, colorants, fragrances, food additives, seasonings). . Specifically, as a nutritional supplement, royal jelly, vitamins, protein, chitosan, lecithin and the like are blended, and a sugar solution and seasoning can be added to adjust the taste. These may be formed into a capsule such as a hard capsule or a soft capsule, a tablet, or a pill, or a powder, granule, bowl, or the like as necessary. These may be eaten as they are, or may be drunk in water, hot water, milk, soy milk, tea, juice or the like according to the shape or preference.

  As long as it has a whitening effect, the blending amount of the kuzu flower extract contained in the whitening agent of the present invention can be appropriately set in a wide range depending on various conditions such as the dosage form used and the object to be used. Preferably, the Kuzuka extract is contained in the external preparation in a proportion of 0.00001% by mass to 20% by mass, more preferably 0.001% by mass to 10% by mass in terms of dry mass.

  The whitening agent characterized by containing the kuzuhana extract of the present invention has a whitening effect superior to, for example, the same amount of isoflavones or saponins, or a plant extract containing the same amount of isoflavones or saponins. Yes.

  The whitening cosmetic of the present invention may contain various medicinal ingredients, humectants, surfactants and other auxiliaries depending on the purpose of enhancing the whitening action of the kuzuhana extract or the purpose of use of the cosmetic. . These auxiliaries may be used alone or in combination of two or more.

Examples of the medicinal component that is one of the auxiliary agents include vitamins, polyphenols, cell activators and phospholipids. Vitamins include vitamins A such as retinol, retinal, retinoic acid, 3-dehydroretinol, 3-dehydroretinal, 3-dehydroretinoic acid; pros such as α-carotene, β-carotene, γ-carotene and cryptoxanthine. Vitamin A, thiamine (vitamin B1), riboflavin (vitamin B2), pyridoxine, pyridoxal, pyridoxamine (above vitamin B6), cobalamin (vitamin B12), nicotinic acid, nicotinamide, pantothenic acid, biotin (vitamin H), folic acid ( Vitamin B) such as vitamin M), ascorbic acid (vitamin C); vitamin D such as ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), 7-dehydrocholesterol, ergosterol, etc. Vitamin E such as rovitamin D, α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol, δ-tocotrienol, phylloquinone (vitamin K1), menaquinone ( Vitamin Ks such as vitamin K2) and menadione (vitamin K3). As the vitamins, any of those derived from natural products such as animals, plants, algae and microorganisms, and synthetic products utilizing chemical reactions or enzymatic reactions can be used. Furthermore, you may use the extract of the natural product containing these as it is.

  Examples of polyphenols include catechol, catechin, epicatechin, gallocatechin, epigallocatechin, gallocatechin gallate, epigallocatechin gallate, tannic acid, hammelitannin (1,5-digalloyl hamamelose), proanthocyanidins, Examples include caffeic acid derivatives, gallic acid, pyrogallol tannin, catechol tannin and the like. Tea extract, grape seed extract, pine bark extract, sweet potato stem and leaf extract and the like containing these polyphenols can also be used as polyphenols.

  Cell activators include yeast extract, Asparagus plant, Avocado (Persea americana Mill.), Aloe plant, Apricot (Prunus armenica L. var. Ansu Maxim.), Ginkgo (Ginkgo). L.), Japanese beech (Fagus japonica Maxim.), Onion garlic (Allium sativum L. f. Pekinense Makino), Panax ginseng C. A. Meyer, Camisole (Mat. ) And Phellodendron plants, cucumber (Cuc) mis sativas L., calendula arvensis L., Lentinus edodes Sing., Actinidia chinensis Pran., Eguisetum p. Garlic (Allium sativum L.), Sembria (Swartia japonica Makino), Tamazaki cephalantha Hayata, Lisa (Lactuca sativa um), L ndula officinalis L., Carcinus (Aesculus turbinata Blume), carrot (Daucus carata L.), pine phoenix (Poria cos wolf L), grape (Vitis vinifera L M. Roemen, safflower (Carthamus tinctorius L.), mannenro (Rosmarinus officinalis L.), Citrus genus plant, Sapindus mukurossi Gaertn. ), Murasaki (Lithosperum officinalale L. var. Erythrorohizon Maxim.), Eucalyptus genus plants, Lilyum genus plants, and the like.

  Phospholipids include phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol (cardiolipin), glycerophospholipids such as phosphatidic acid, lysophosphatidylcholine (lysolecithin), lysophosphatidylethanolamine, lysophosphatidylserine, Phosphatidylinositol, lysophosphatidylglycerol, lysoglycerophospholipids such as lysophosphatidic acid, sphingophospholipids such as sphingomyelin; and hydrogenated products thereof. These phospholipids may be derived from animals and plants such as soybeans and egg yolks, or may be synthesized by a chemical or enzymatic method.

Examples of humectants include collagen or its degradation products, soy peptides, amino acids, mucopolysaccharides such as hyaluronic acid, amino sugars such as chondroitin, saccharides such as trehalose, and water-soluble substances such as seaweed, alginic acid, glucomannan, and pectin. Dietary fiber. Examples of the surfactant include lecithin, fatty acid ester, and amino acid derivative.

  The whitening cosmetic product of the present invention has a whitening effect superior to a whitening cosmetic product containing, for example, the same amount of isoflavones or saponins, or a plant extract containing the same amount of isoflavones or saponins.

The present invention will be described below with reference to examples, but it goes without saying that the present invention is not limited to these examples.

As the Kuzuhana extract, Kuzuka extract (containing 20% by weight of isoflavone) manufactured by Toyo Shinyaku Co., Ltd. was used. As the soybean extract, a soybean extract (containing 40% by weight of isoflavone) manufactured by Fuji Oil Co., Ltd. was used.

Example 1
Example 1 demonstrates the melanin production inhibitory effect of this invention. In Example 1, it is confirmed by the measurement of melanin production inhibition rate that the present invention suppresses melanin production. Hereinafter, a specific method of measurement will be described.

Theophylline (manufactured by Sigma) was dissolved in DMSO (manufactured by Wako Pure Chemical Industries, Ltd.) to a concentration of 133.4 mM. Further, it was diluted 200 times with a normal medium (D-MEM containing 10% fetal bovine serum: Sigma) to obtain a test medium.

The concentration of Kuzuhana extract was adjusted with a normal medium and filtered and sterilized with a 0.2 μm filter. A positive control was prepared by dissolving kojic acid in distilled water, adjusting the concentration in a test medium, and then sterilizing by filtration with a 0.2 μm filter. As a negative control, a test medium alone was sterilized by filtration with a 0.2 μm filter.

Preparation of B16 melanoma cells used for evaluation of the melanin production inhibitory effect was performed as follows. Mouse-derived frozen B16 melanoma cells (RCB1283: RIKEN) were thawed using a normal medium, seeded in a 6-well plate, and then cultured in a carbon dioxide incubator. Further, the cells were subcultured from a 6-well plate to a 75 cm ² flask, and the culture was continued in a carbon dioxide incubator. Thereafter, the cells were collected from the 75 cm ² flask, seeded in a 6-well plate, and cultured for 24 hours in a carbon dioxide incubator. After removing the medium from the plate, a medium containing a test substance was added at 1.5 ml / well, and a test medium was further added at 1.5 ml / well, followed by culturing for 3 days.

After culturing, the cell culture supernatant is removed from each well, the cells are washed once with 2 ml of phosphate buffer (PBS (−)), and Trypsin-EDTA (manufactured by Sigma) diluted with PBS (−) is added. 500 μl / well was added. After treatment at room temperature for 4 to 5 minutes, PBS (-) containing 10% fetal bovine serum was added at 500 μl / well to neutralize trypsin. Thereafter, the cells were detached by pipetting, and the cells were collected. After centrifuging at 2,000 rpm for 5 minutes and removing the supernatant, 1 ml of PBS (−) was added and suspended, and then 60 μl of the suspension was sampled to count the number of cells. The remaining cells were centrifuged and the supernatant was completely removed. 250 μl of 1% Triton X-100 / 1N sodium hydroxide aqueous solution was added to the cells, heated in a thermostatic bath at 65 ° C. to 75 ° C. for 60 minutes, and then shaken to completely dissolve.

The melanin production inhibitory action was evaluated as follows. The obtained B16 melanoma cell lysate derived from a mouse was transferred to a 96-well plate at 200 μl / well, the amount of synthetic melanin of each substance was measured at a wavelength of 490 nm, and the inhibition rate obtained from the following formula was expressed.
Inhibition rate (%) = 100− (absorbance of test substance / absorbance of negative control) × 100

The results are shown in Table 1.

  From the results in Table 1, it can be seen that the Kuzuka extract has an excellent melanin production inhibitory effect. That is, the melanin production inhibitory effect obtained from the Kuzuhana extract is very superior to the inhibitory effect of the soybean extract containing the same amount of isoflavones. These show that the Kuzuka extract used in the present invention has an excellent effect as compared with isoflavones conventionally known as melanin production inhibitors or plant extracts containing isoflavones. Therefore, the Kuzuhana extract exhibits an excellent whitening effect and is useful as a whitening agent.

(Example 2)
Example 2 describes tyrosinase activity inhibition according to the present invention. In Example 1, it is confirmed by measurement of tyrosinase activity inhibition that the present invention has tyrosinase activity inhibition. Hereinafter, a specific method of measurement will be described.

First, the concentration of Kuzuka extract was prepared using 1/15 M phosphate buffer (pH 7.2) to obtain a sample solution. Similarly, the concentration of kojic acid was prepared and used as a positive control.

  The enzyme solution was prepared as follows. Tyrosinase (derived from mushrooms, manufactured by CALZYME) was prepared using a 1/15 M phosphate buffer (pH 7.2) to 550 units / ml to obtain an enzyme solution.

  The substrate solution was prepared as follows. An L-DOPA solution (manufactured by Wako Pure Chemical Industries, Ltd.) was prepared to be 0.3 mg / ml using a 1/15 M phosphate buffer (pH 7.2), and used as a substrate solution.

  The test solution was prepared as follows. After adding the sample solution to the 96-well plate at 100 μl / well, the enzyme solution was added at 100 μl / well and incubated at 37 ° C. for 10 minutes. Furthermore, the substrate solution was added at 100 μl / well and incubated at 37 ° C. for 5 minutes, and then the absorbance at 475 nm was measured.

  The test solution blank was prepared as follows. After adding the sample solution to the 96-well plate at 100 μl / well, the enzyme solution was added at 100 μl / well and incubated at 37 ° C. for 10 minutes. Furthermore, instead of the substrate solution, 1/15 M phosphate buffer (pH 7.2) was added at 100 μl / well, incubated at 37 ° C. for 5 minutes, and then the absorbance at 475 nm was measured.

  The control solution was prepared as follows. After adding 1 / 15M phosphate buffer (pH 7.2) to a 96-well plate at 100 μl / well, an enzyme solution was added at 100 μl / well and incubated at 37 ° C. for 10 minutes. Furthermore, the substrate solution was added at 100 μl / well and incubated at 37 ° C. for 5 minutes, and then the absorbance at 475 nm was measured.

  The control solution blank was prepared as follows. After adding 1 / 15M phosphate buffer (pH 7.2) to a 96-well plate at 100 μl / well, an enzyme solution was added at 100 μl / well and incubated at 37 ° C. for 10 minutes. Further, 1/15 M phosphate buffer (pH 7.2) was added at 100 μl / well, and incubated at 37 ° C. for 5 minutes, and then the absorbance at 475 nm was measured.

Tyrosinase activity inhibition was expressed as an inhibition rate obtained from the following formula.
Inhibition rate (%) = [100 − [(AB) / (CD)] × 100]
A: Absorbance at 475 nm of the test solution.
B: Absorbance at 475 nm of the test solution blank.
C: Absorbance at 475 nm of the control solution.
D: Absorbance at 475 nm of the control solution blank.

The results are shown in Table 2.

  From the results in Table 2, it can be seen that the Kuzuka extract has an excellent tyrosinase activity inhibitory effect. That is, the tyrosinase inhibitory effect obtained from the Kuzuhana extract is very superior to the inhibitory effect of the soybean extract containing the same amount of isoflavones. These indicate that the Kuzuka extract used in the present invention has an excellent tyrosinase inhibitory effect as compared to isoflavones conventionally known as tyrosinase inhibitors or plant extracts containing isoflavones. Therefore, the Kuzuhana extract exhibits an excellent whitening effect and is useful as a whitening agent.

As described above, the whitening agent characterized by containing the Kuzuka extract relating to the present invention has a tyrosinase activity inhibitory action and a melanin production inhibitory action. Therefore, cosmetics and foods exhibiting an excellent whitening effect can be obtained by including this whitening agent in cosmetics and foods. The whitening agent described in the present invention can be used as cosmetics, foods, and the like by using the Kuzuka extract as it is or by adding various components.

  The whitening agent characterized by containing the Kuzuka extract relating to the present invention has a tyrosinase activity inhibitory action and a melanin production inhibitory action. Therefore, cosmetics and foods exhibiting an excellent whitening effect can be obtained by including this whitening agent in cosmetics and foods. The whitening agent described in the present invention can be used as cosmetics, foods, and the like by using the Kuzuka extract as it is or by adding various components.

Claims (4)

Whitening agent characterized by containing Kuzuhana extract. The whitening agent according to claim 1, wherein the Kuzuhana extract has a melanin production inhibitory action. The whitening agent according to claim 1, wherein the Kuzuhana extract has a tyrosinase activity inhibitory action. A whitening cosmetic comprising the whitening agent according to claim 1.