JP2010270012A - Sirtuin 1 activating agent, sirtuin 1 activating composition, and external preparation for skin, cosmetic, food compounded with the sirtuin 1 activating agent or sirtuin 1 activating composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a sirtuin 1 activating agent which has excellent sirtuin 1 activating ability and does not have such a problem that conventional resveratrol is oxidized and browned with heat, oxygen and light to deteriorate medicinal effects, to provide an activating composition, to provide an external preparation for skin, to provide a cosmetic, and to provide food. <P>SOLUTION: The sirtuin 1 activating agent is characterized by containing a pueraria root extract as an activating agent. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention includes a sirtuin 1 (hereinafter also referred to as SIRT1) activator and an activation composition involved in the suppression of natural aging and life extension, and the ability to activate such sirtuin 1 to The present invention relates to external preparations for skin, cosmetics, and foods that exhibit an inhibitory action.

  The ability to control the speed of natural aging has been demonstrated through various model organisms. It has long been known that aging inhibition is caused by calorie restriction. In recent years, however, genes for extending life span have been identified in various species such as yeast, nematodes, flies and rodents. These genes are considered to be one of the evolutionarily conserved pathways that have evolved to increase survival in response to deteriorating environmental conditions (such as caloric restriction).

  Yeast has proven to be a useful model for studying longevity. In yeast, life span is defined by the number of divisions of individual cells. Yeast sirtuin 2 (Silent Information Regulator 2: hereinafter also referred to as SIRT2) gene is involved in life extension, and when this gene is additionally introduced into yeast, it is recognized that life span is extended by about 30 to 70%. . It has been proved that the SIRT2 gene is also necessary for the life extension effect by calorie restriction of yeast.

  The human homologue of yeast SIRT2 is named SIRT1-7 (SIR Two ortholog-1-7). Among them, SIRT1 is considered to be functionally similar to yeast SIRT2. Non-Patent Document 1 below also describes that when SIRT1 gene is additionally introduced into human cells, the amount of Senescence Associated-β-galactosidase (SA-β-gal), which is an indicator of aging, is reduced. . This indicates that SIRT1 is effective in suppressing aging even in mammalian cells.

  SIRT1 is known as a deacetylase. SIRT1 is a member of the sirtuin family that requires NAD + among deacetylases. It is known that the enzyme activity of SIRT1 as a deacetylase is activated by polyphenols having a transstilbene structure such as resveratrol, butein, piceantannol, fisetin, and quercetin.

  It is known that when SIRT1 is activated, it exhibits a life extension action and prevention of cell death, and thus exhibits an anti-aging effect that suppresses natural aging and prolongs life. Resveratrol is known as a component that strongly activates SIRT1 (Patent Document 1 and Patent Document 2 below).

  The present inventors thought that aging of skin can be suppressed by activating SIRT1 present in skin cells. Actually, resveratrol, which is a SIRT1 activator, was added to skin fibroblasts, and the amount of SA-β-gal, which is an indicator of aging, was found to decrease. Therefore, it was confirmed that cosmetics and foods containing the SIRT1 activator have an anti-aging effect.

  However, resveratrol has a problem that it is oxidized or browned by heat, oxygen and light, and its medicinal effect is lowered. Accordingly, there is a demand for the development of active ingredients and compositions that are excellent in SIRT1 activation ability and stability in place of resveratrol.

JP 2007-326872 A JP 2006-298776 A

(1) PLOS ONE. 2008 Mar 5; 3 (3): e1710

  The present invention was made to solve such problems, and is oxidized or browned by heat, oxygen and light like the above conventional resveratrol, and there is no problem that the medicinal effect is reduced, Moreover, it is an object to provide a sirtuin 1 activator having an excellent ability to activate sirtuin 1, an activation composition, an external preparation for skin, a cosmetic, and a food.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that the konkon extract has excellent SIRT1 activation ability and excellent stability, and have completed the present invention.

  That is, this invention provides the sirtuin 1 activator characterized by using a cuckoo extract as an active ingredient. Moreover, this invention provides the sirtuin 1 activation composition characterized by containing such a cuckoo extract.

  In such a sirtuin 1 activation composition, it is also possible to contain resveratrol in addition to the cuckoo extract. Moreover, it is also possible to contain a grape vine extract in addition to the cuckoo extract. Furthermore, it is possible to contain daidzein in addition to the cuckoo extract. Furthermore, chitosan or a chitosan derivative can also be contained.

  The kacon extract can be extracted with, for example, ethanol.

  Furthermore, this invention provides the skin external preparation, cosmetics, and foodstuff which have the sirtuin 1 activation ability containing the above sirtuin 1 activators or the sirtuin 1 activation composition.

  According to the present invention, as described above, sirtuin 1 replaces conventional resveratrol by using cuckoo extract as an active ingredient of sirtuin 1 activator, or by including cuckoo extract in a sirtuin 1 activation composition. It came to be able to provide the activator or the activation composition excellent in the activation ability.

  In addition, the use of the cocoon extract has an effect that the problem that the medicinal effect is lowered due to oxidation or browning caused by heat, oxygen and light generated in conventional resveratrol can be solved.

  As described above, the sirtuin 1 activator of the present invention comprises a cuckoo extract as an active ingredient. Moreover, the sirtuin 1 activation composition of this invention contains a cuckoo extract as mentioned above.

  Cuckon extract is an extract of the roots of Kudzu (Pueraria lobata Ohwi), a kind of perennial vine. The extraction method of the cuckoo extract is not particularly limited, but can be extracted with the solvent from the root of Kudu (Pueraria lobata Ohwi) as it is or after pulverization, from room temperature to the boiling point of the solvent.

  Usable solvents are water, alcohols (for example, lower alcohols such as ethanol, butanol and methanol, polyhydric alcohols such as 1,3-butylene glycol and glycerin), ketones such as acetone, ethyl ether, dioxane and acetonitrile. , Esters such as ethyl acetate, and organic solvents such as xylene and benzene can be used alone or in any combination of two or more. Further, depending on the purpose of use, it may be diluted or evaporated to form a paste or processed into a dry powder.

  Moreover, it is preferable to use an ethanol extract as the cool extract. This is because the ethanol extract exhibits a high SIRT1 activation ability. As the ethanol extract, for example, puerarin extract B (trade name: manufactured by Ikeda Sakuka Kogyo Co., Ltd.) can be used.

  The sirtuin 1 activating composition of the present invention may contain resveratrol in addition to the cuckoo extract as described above. Resveratrol is a kind of polyphenol present in grape leaves and shoots, and is a general term for trans-resveratrol, cis-resveratrol and mixtures thereof. It is also possible to use a component containing resveratrol.

  As the component containing resveratrol, it is preferable to use a component containing 0.1% by weight or more of resveratrol. Examples of the component containing resveratrol include grape vine extract. As the grape vine extract, for example, Resveratrox (trade name: manufactured by Sunbright Co., Ltd.), which is a grape vine extract containing 1.0 to 3.0% by weight of resveratrol, can be used. In addition to resveratrol, biniferin, which is a dimer of resveratrol, can also be used. Examples of the grape vine extract containing such vinyliferin include Viniferol (trade name: manufactured by Sun Bright).

  The sirtuin 1 activation composition of the present invention may contain daidzein. Daidzein is an isoflavone aglycone that is abundant in soybeans. Examples of extracts containing daidzein include soybean extract.

  Furthermore, the sirtuin 1 activation composition of the present invention may contain chitin / chitosan or a derivative thereof. By containing such chitin / chitosan or a derivative thereof, the stability of the SIRT1 activator of the present invention can be enhanced. The types of chitin / chitosan and their derivatives are not particularly limited. For example, carboxymethylchitin, carboxymethylchitosan, glycolate of chitosan, pyrrolidone carboxylate, salts of lactate and ascorbate, partially myristoylated chitosan pyrrolidone Examples thereof include carboxylic acids (for example, PM-chitosan (trade name: manufactured by Pierce Co., Ltd.), partially myristoylated carboxymethyl chitosan, and the like.

  In addition, components commonly used in cosmetics, external preparations, and foods can be blended in a range that does not impair the effects and functionality of the present invention, depending on the form. For example, polyhydric alcohols such as butylene glycol, dipropylene glycol and glycerin, higher alcohols such as cetanol and behenyl alcohol, nonpolar oils such as liquid paraffin and squalane, and ester oils such as isopropyl palmitate and isopropyl myristate And liquid oils such as vegetable oils such as wheat germ oil and olive oil, silicon compounds such as trimethylsiloxysilicic acid and methylphenylpolysiloxane, and fluorine compounds such as perfluoropolyether. Water-soluble polymers, moisturizing and softening agents, antioxidants, astringents, whitening agents, antibacterial agents, anti-inflammatory agents, UV absorbers, UV scattering agents, water-soluble vitamins, oil-soluble vitamins, enzymes, etc. Ingredient standards for quasi-drugs, blended ingredient-specific specifications for cosmetic varieties, cosmetic ingredients standards, Japanese pharmacopoeia, official standards for food additives, and the like.

  As cosmetics, for example, 1) topical or systemic skin cleansers or skin cosmetics, 2) medicinal and / or cosmetic preparations applied to the scalp and hair, and 3) used in bath water Bath agent, 4) Deodorant and deodorant for human body, 5) Oral agent such as mouthwash, 6) Sheet for skin application, cosmetic sheet, cosmetic cotton, wet tissue, etc., solid, solution , Gels, emulsions, sheets, sprays, ampoules, capsules, powders, etc.

  Specifically, lotions, emulsions, creams, ointments, gels, cosmetics, facial impregnation sheets, basic cosmetics such as oils, packs, mists, skin cleansing agents such as facial cleansers, cleansings, shampoos, rinses, hair treatments Hair cosmetics such as hair nick, hair growth and nourishing hair, makeup cosmetics such as foundation, lipstick, eye shadow, eyeliner, mascara, etc.

  An emulsified cosmetic can be prepared by emulsifying a liquid oil agent. Specific examples include liquid paraffin, squalane, isononyl isononanoate, isotridecyl isononanoate, cetyl ethylhexanoate, glyceryl tri-ethylhexanoate, glycerin tri (capryl / capric acid), cetyl palmitate, isostearic acid-2- Hexyldecyl, stearyl stearate, isostearyl myristate, di-2-ethylhexanoic acid neopentyl glycol, dicapric acid neopentyl glycol, diisostearate glyceryl, propylene glycol distearate, and other liquid oils such as rice oil, rice bran oil, Primrose oil, linoleic acid, wheat germ oil, olive oil, jojoba oil, grape seed oil, chamomile oil, rosemary oil, fennel oil, and other animal liquid oils such as jojoba oil and egg yolk oil. It is.

  In this case, as the emulsifier, nonionic surfactant such as fatty acid monoglyceride, polyoxyethylene fatty acid ester, polyoxyethylene alkyl ether, polyglycerin fatty acid ester, cationic surfactant such as benzalkonium chloride, amphoteric surfactant Agents, anionic surfactants and the like can be used.

  Furthermore, the sirtuin 1 activation composition of the present invention may contain a water-soluble polymer. Examples of water-soluble polymers include gum arabic, quinseed, agar, casein, dextrin, gelatin, pectin, starch, carrageenan, alginic acid or a salt thereof, hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof, ethyl cellulose, methyl cellulose, carboxymethyl cellulose And carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, polyvinyl alcohol, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, carboxyvinyl polymer, polyethyleneimine and the like.

  The food of the present invention includes beauty foods. Here, beauty food means all foods, beverages and internal preparations for beauty and health, and the forms are ampoules, capsules, tablets (pellets), powders, granules, solids, liquids, jelly (gels), etc. can do. Specifically, in addition to nutritional supplementation, nourishing tonic, fatigue recovery, constitution improvement, whitening, skin beautification, hair restoration / hair growth, slimming, mental stability, etc., beauty and health oriented foods and beverages, gum, candy, yogurt, soft drinks Preference foods or drinks, such as water, are mentioned.

  As pigments and colorants used in beauty foods, grape juice pigment, red cabbage pigment, red rice pigment, Anato pigment, squid ink pigment, turmeric pigment, krill pigment, amber pigment, gardenia pigment, onion pigment, spirulina pigment, hibiscus pigment Purple potato pigment, purple yam pigment, rutin and the like.

  Other sweeteners and nutrient enhancers include sugar, fructose, arabinose, xylobiose, galactose, glucosamine, collagen, xylose, trehalose, acetylglucosamine, amino acids such as proline and arginine, fructooligosaccharides, stevia, mannose, maltose, honey, Glucose, miraculin, monelin, licorice extract, shell calcined calcium, yeast, wheat germ, hemicellulose, heme iron and the like can be used.

  As water-soluble vitamins used in cosmetics and beauty foods, vitamin B group thiamine hydrochloride, thiamine sulfate, riboflavin, pyridoxine cyanocobalamin, folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositol, etc. Ascorbic acid or a derivative thereof which is a vitamin C group, carnitine, ferulic acid, or the like can be used, and vitamin Ps such as rutin, eliocitrin, hesperidin, or the like can be used.

  Further, as oil-soluble vitamins used in cosmetics and beauty foods, vitamin A group such as retinol, retinal, dehydroretinal, β-carotene, lycopene, astaxanthin, retinol palmitate, etc. can be used. Ergocalciferol, cholecalciferol, dihydrotaxosterol etc. can be used, and tocopherol or its derivative etc. which are vitamin E group can be used. In addition, vitamin F, γ-oryzanol, etc. Can be used.

  Examples of the present invention will be described below.

(Reference example)
In human skin cells, it is known that the amount of SA-β-galactosidase in cells increases with age. Therefore, SA-β-galactosidase is used as a marker for cell senescence. Using human skin-derived fibroblasts that had been subcultured for 4 months, a test using SA-β-galactosidase staining was conducted for the suppression of aging of resveratrol (Sigma), which is generally used as a SIRT1 activator. .

  After constant cell density, the cells were exposed for 4 days in a medium containing resveratrol (10-30 μM). Cell Senescence detection kit (manufactured by Cosmo Bio) was used for SA-β-galactosidase staining. The amount of SA-β-galactosidase staining was measured and the amount not containing resveratrol was measured in the same manner as a control. The amount of SA-β-galactosidase staining for the control was taken as 100, and the ratio of the amount of SA-β-galactosidase staining to the control was Asked. The measurement results are shown in Table 1. As is clear from Table 1, it was suggested that the addition of resveratrol significantly suppresses SA-β-galactosidase staining. Furthermore, as a result of binarizing the stained image and quantifying the amount of SA-β-galactosidase, it was found that the amount of SA-β-galactosidase was reduced by 18 to 23% by adding resveratrol. Thus, it was confirmed that resveratrol that activates SIRT1 is effective for suppressing skin aging due to aging. From this, it was suggested that the SIRT1 activator is effective for suppressing skin aging due to aging.

Example 1
In this example, the SIRT1 activity of the cuckoo extract, which is the SIRT1 activator of the present invention, was tested. In the test, SIRT1 Fluorescent Activity Assay / Drug Discovery Kit (BIOMOL) was used. In this kit, a fluorogenic peptide based on a sequence containing the 382rd lysine deacetylated by SIRT1 in the amino acid sequence of p53 is used as a substrate, and this peptide is deacetylated by SIRT1. Since the fluorescence is emitted at the same time, the enzyme activity of SIRT1 can be measured.

  And by adding various substances to this reaction system, SIRT1 inhibitors and SIRT1 activators can be used for screening. As a result of screening more than 250 components, it was found that the Kakonkon extract has SIRT1 activity. Cascon extract (manufactured by Ikeda Sakuka Kogyo Co., Ltd., product name: Puerarin Extract B) was prepared to a final concentration of 0.25 wt%, 0.05 wt%, 0.025 wt%, and SIRT1 Fluorescent Activity Assay / Drug Discovery The SIRT1 activity was measured using a kit, and purified water was similarly measured as a control. The SIRT1 activity of the control was taken as 100, and the ratio of the SIRT1 activity to the control was determined. The measurement results are shown in Table 2.

  As is clear from Table 2, the SIRT1 activity of the cocoon extract showed 7.3 times, 2.25 times and 1.8 times the activity of the control, respectively. It was also found that the higher the final concentration, the higher the SIRT1 activity. From this result, it was revealed that the cuckoo extract significantly increased the deacetylation activity of SIRT1.

  Resveratrol was also measured in the same manner, and it was found that the SIRT1 activity of the cuckoo extract is comparable to resveratrol, which is known as an excellent activator of SIRT1.

(Comparative Example 1)
Soybean extract, rosemary extract, clove extract, button bili extract, ryokucha extract, pycnogenol, turmeric extract, asparagus extract, auren extract, buckwheat extract, kina extract, jujube extract, pearl barley extract, tea The extract was prepared to a final concentration of 0.25% by weight, and SIRT1 activity was measured with SIRT1 Fluorescent Activity Assay / Drug Discovery Kit in the same manner as in Example 2, and purified water was also measured as a control. SIRT1 activity was taken as 100, and the ratio of SIRT1 activity to control was determined. Table 3 shows the measurement results. As is clear from Table 3, no difference from the control was observed, indicating that there was no effect of increasing the deacetylation activity of SIRT1.

(Example 2)
In this example, the SIRT1 activation composition of the present invention containing a cuckoo extract was tested for SIRT1 activity. As a component containing resveratrol, grape vine extract (product name: Resveratrox) containing 1.0 to 3.0% by weight of resveratrol is adjusted to a final concentration of 0.05% by weight. The SIRT1 activity was measured using SIRT1 Fluorescent Activity Assay / Drug Discovery Kit, and purified water was similarly measured as a control. The SIRT1 activity of the control was taken as 100, and the ratio of SIRT1 activity to the control was determined. Table 4 shows the measurement test results. As is clear from Table 4, the grape vine extract showed 1.35 times the activity of the control.

  Grape vine having 0.05% by weight of cuckon extract (trade name: Puerarin extract B, manufactured by Ikeda Saccharification Co., Ltd.) having 2.25 times the SIRT1 activation ability of the control and 1.35 times the activation ability of the control. An SIRT1 activation composition was prepared by coexisting with 0.05% by weight of an extract (product name: Resveratrox, manufactured by Sunbright), and the SIRT1 activity of the SIRT1 activation composition was measured. As a result of the measurement, the activity was 3.25 times that of the control. From this result, it was clarified that the activity of SIRT1 is synergistically increased by coexisting the cuckoo extract and the grape vine extract containing resveratrol.

  In addition, 0.05 wt% cascon extract (product name: Puerarin Extract B, manufactured by Ikeda Saccharification Co., Ltd.) having 2.25 times the SIRT1 activation ability of the control and 3.1 times activation ability of the control. Resveratrol (0.025 mM) was allowed to coexist to form a SIRT1 activation composition, and the SIRT1 activity of the SIRT1 activation composition was measured. As a result of the measurement, the activity was 5.15 times that of the control. Further, when SIRT1 activation composition was prepared by coexisting 0.025% by weight of cocoon extract and resveratrol (0.025 mM) and SIRT1 activity was measured in the same manner, it showed 4.3 times the activity of the control. It was. From these results, it was revealed that the activity of SIRT1 is synergistically increased by coexistence of the cuckoo extract and resveratrol.

  Furthermore, it has 0.05% of Cakkon extract (product name: Puerarin extract B, manufactured by Ikeda Saku Kagaku Kogyo Co., Ltd.) having 2.25 times the SIRT1 activation ability of the control and 2.7 times the activation ability of the control. SIRT1 activation composition was prepared by coexisting daidzein (0.025 mM), and SIRT1 activity of the SIRT1 activation composition was measured. As a result of the measurement, the activity was 4.95 times that of the control. From this result, it was clarified that the activity of SIRT1 is synergistically increased by coexistence of the cuckoo extract and daidzein.

  From these results, it was found that the SIRT1 activation composition in which the cuckoo extract and resveratrol, grape vine extract, or daidzein coexist was an effective composition for activating SIRT1. This proved useful for cosmetics, external preparations, and beauty foods having an anti-aging function that suppresses aging due to aging of the skin.

Example 4
In this example, the stability of the activation ability of SIRT1 was tested. After measuring the activation ability of SIRT1, it was measured at 40 ° C. after 2 months. The measurement results are shown in Table 5. As is clear from Table 5, resveratrol decreased the activation ability of SIRT1, whereas the cuckoo extract showed almost no decrease in activation ability, and was an active ingredient with excellent drug stability. I found out.

(Formulation Example 1) (Anti-aging Essence)
This formulation example is an example of a cosmetic liquid that is cloudy and does not contain a low-molecular-weight surfactant as an example, and the composition and blending ratio are as follows.

Composition ratio (wt%)
Mineral oil 1.0%
Squalane 1.0%
Partially myristoylated carboxymethyl chitosan 0.2%
Butylene glycol 2.5%
Dipropylene glycol 5.5%
Methylparaben 0.1%
Cuckoo extract
(Ikeda saccharification, puerarin extract B-2, solution type) 5.0%
Cuckoo extract (powder type) 0.03%
Total amount of purified water remaining 100.0%

  The cloudy anti-aging essence was prepared as follows. That is, mineral oil and squalane, which are oil phases, are heated and dissolved at 70 ° C., and then gradually added to an aqueous phase composed of partially myristoylated carboxymethyl chitosan, butylene glycol, etc., and homomixer (8000 rpm, 5 minutes treatment) Evenly dispersed. The mixture was cooled to 40 ° C. under deaeration, added with a konkon extract or the like as an SIRT1 activation component, and uniformly dispersed with a homomixer (3000 rpm, 1 minute treatment). A cloudy aqueous essence was obtained by deaeration and filtration.

(Formulation Example 2) (Anti-aging Essence)
This Formulation Example is a formulation example of a cosmetic liquid that is white turbid and does not contain a low-molecular-weight surfactant, as in Formulation Example 1.

Composition ratio (wt%)
Mineral oil 1.0%
Propyl myristate 1.0%
Partially myristoylated carboxymethyl chitosan 0.2%
Butylene glycol 5.5%
Propylene glycol 5.5%
Methylparaben 0.1%
Cascon extract (Ikeda saccharification, puerarin extract B-2, solution type) 5.5%
Grape vine extract (product of Sunbright, product name: Resveratrox, powder type) 0.3%
Daidzein 0.05%
Total amount of purified water remaining 100.0%

  The cloudy anti-aging essence was prepared as follows. That is, mineral oil and propyl myristate, which are oil phases, are heated and dissolved at 70 ° C., and then gradually added to an aqueous phase composed of partially myristoylated carboxymethyl chitosan, butylene glycol, etc., and treated with a homomixer (8000 rpm for 5 minutes) ). The mixture was cooled to 40 ° C. under deaeration, added with a konkon extract or the like as an SIRT1 activation component, and uniformly dispersed with a homomixer (3000 rpm, 1 minute treatment). A cloudy aqueous essence was obtained by deaeration and filtration.

(Formulation example 3) Hydrophilic cream (wrinkle prevention cream)
This formulation example is a formulation example of a hydrophilic cream for preventing wrinkles, and the composition and the blending ratio are as follows.

Composition ratio (wt%)
Glyceryl stearate 1.0%
POE (20) cetyl ether 0.3%
Tri (caprylic / capric) glyceryl 1.0%
Behenyl alcohol 4.0%
Propyl myristate 2.5%
Tocopherol acetate 0.3%
Vaseline 1.0%
Butylene glycol 1.5%
Phenoxyethanol 0.2%
Partially myristoylated chitosan pyrrolidone carboxylate 0.3%
Chitosan glycolate 0.1%
Cascon extract (ethanol extraction, powder type) 0.05%
Resveratrol 0.01%
Grape vine extract (powder type) 0.2%
Total amount of purified water remaining 100.0%

  Preparation of a wrinkle-preventing hydrophilic cream was performed as follows. That is, after the oil phase glyceryl stearate, POE (20) cetyl ether, tri (caprylic acid / capric acid) glyceryl, behenyl alcohol, propyl myristate, tocopherol acetate and petrolatum are heated and dissolved at 70 ° C., partially myristoylated chitosan or The mixture was gradually added to an aqueous phase composed of butylene glycol and the like, and uniformly dispersed with a homomixer (8000 rpm, 1 minute treatment). The reaction mixture was cooled to 40 ° C. under deaeration, and the SIRT1 activating components such as cocoon extract and resveratrol were added and dispersed uniformly with a homomixer (2000 rpm, 1 minute treatment). By degassing and filtering, a cloudy hydrophilic cream was obtained.

(Prescription Example 4) Hair Care Liquid (for hair growth)
This prescription example is a prescription example of a hair care liquid for hair growth, and the composition and blending ratio are as follows.

Composition ratio (wt%)
Ethanol 30.0%
Stearyltrimethylammonium chloride 0.8%
POE (20) hydrogenated castor oil 0.2%
Partially myristoylated carboxymethyl chitosan 0.3%
Propylene glycol 7.0%
Glycolic acid 0.3%
Tocopherol acetate 0.5%
Cascon extract (ethanol extraction, solution type) 7.8%
Grape vine extract (powder type, containing 2.5% resveratrol) 0.2%
Total amount of purified water remaining 100.0%

(Formulation example 5) Beauty supplement (tablet)
This prescription example is a prescription example of a cosmetic supplement, and the composition and blending ratio are as follows. And the composition of the following composition is tableted and it is set as a tablet.

Composition ratio (wt%)
Glucosamine 0.5%
Collagen powder 3.0%
Arginine 3.0%
Fructooligosaccharide, 5.0%
Cuckoo extract (ethanol extraction, powder type) 1.0%
Grape vine extract (powder type, containing 2.5% resveratrol) 1.5%
Chitosan glutamate 1.0%
Chitin powder 0.5%
Corn starch 30%
Hydroxypropyl cellulose 3.0%
Magnesium stearate 1.5%
Ascorbic acid 1.0%
Lactose balance 100%

(Prescription Example 6) Beauty / Health Drink This prescription example is a prescription example of a beauty / health drink, and the composition and blending ratio are as follows.

Composition ratio (wt%)
Fructooligosaccharide 5.0%
Cascon extract (ethanol extraction, powder type) 0.1%
Grape vine extract (powder type, containing 2.5% resveratrol) 0.1%
Ascorbic acid 0.1%
Citric acid 0.2%
Sorbitol 0.8%
Acetylglucosamine 0.5%
Purified water balance 100.0%

Claims (11)

  A sirtuin 1 activator comprising a cocoon extract as an active ingredient.   The sirtuin 1 activator according to claim 1, wherein the cuckoo extract is extracted with ethanol.   The sirtuin 1 activation composition characterized by containing a cuckoo extract.   The sirtuin 1 activating composition according to claim 3, further comprising resveratrol.   The sirtuin 1 activation composition of Claim 3 or 4 which further contains a grape vine extract.   The sirtuin 1 activation composition according to any one of claims 3 to 5, further comprising daidzein.   The sirtuin 1 activation composition of Claims 3 thru | or 6 which is what a cascon extract is extracted with ethanol.   The sirtuin 1 activation composition according to any one of claims 3 to 7, further comprising chitosan or a chitosan derivative.   A sirtuin 1 activating agent according to claim 1 or 2, or a sirtuin 1 activating composition according to any one of claims 3 to 8, which is a skin external preparation having sirtuin 1 activating ability.   A sirtuin 1 activating agent according to claim 1 or 2, or a sirtuin 1 activating composition according to any one of claims 3 to 8, wherein the cosmetic has sirtuin 1 activating ability.   A sirtuin 1 activating agent according to claim 1 or 2, or a sirtuin 1 activating composition according to any one of claims 3 to 8, wherein the sirtuin 1 activating food is provided.