JP2015157773A - cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic that improves a skin barrier function to form a healthy skin while forming a flexible and stretchable skin by inhibiting aging of dermal fibroblasts, the cosmetic having a whitening effect together, by providing various agents that have no side effects and is excellent in safety.SOLUTION: The fermentation product obtained by fermenting Lonicera japonica (Caprifoliaceae Lonicera Japanese honeysuckle) with lactic acid bacteria is used. The lactic acid bacteria is preferred to be Lactobacillus plantarum. The fermentation product obtained by fermenting Lonicera japonica with lactic acid bacteria has a strong cell differentiation promotion effect, barrier function improving effect, cell aging inhibitory effect, SA-β-Gal activity inhibitory effect, and whitening effect as compared with Lonicera japonica extract before fermentation treatment, and is useful.

Description

The present invention is various agents comprising fermented products obtained by fermenting gold and silver flowers with lactic acid bacteria as active ingredients, cell differentiation promoting effect, barrier function improving effect, cell aging inhibiting effect, SA-β-Gal activity inhibiting effect, The present invention relates to a cosmetic containing a metabolite having a high melanin production inhibitory effect as an active ingredient.

Gold and silver flowers refer to the florets or flower parts of the genus Lonicera japonica Thunb. And have been used for a long time. Honeysuckle, also known as Shinobu Winter, has been used as a crude drug in the foliage from autumn to winter. The original plant honeysuckle is a perennial vine that grows in the sunny areas of Yamano. It is derived from the meaning of “Kazura”. In addition, the Chinese name “Shinofu” is derived from the fact that all leaves do not fall even in winter.

Specifically, as an example of using fermented gentian, Patent Document 1 describes a method for producing processed foods containing sesame as the main ingredient. Fermentation of sesame seeds with vegetables, fruits, and wild grasses (nindo) using yeast and lactic acid bacteria. A method for producing a food to be processed is described. However, the content of the patent is mainly to cut the sugar of the glycosylated product of sesame component by fermentation, and to enhance the strong antioxidant effect of sesame and the enhancing function of liver function. There is no mention of any effect.

In Patent Document 2, as a food for animals such as pets and livestock, fermenting wild grasses, vegetables or fruits, deer anteater, etc. with a fermented liquid containing yeast and lactic acid bacteria, the pet's constitution has a strange odor. It is described to provide animal foods that prevent them from occurring. However, even in this patent document, the effect of fermenting Nindo is not described except for the deodorizing effect.

Furthermore, Patent Document 3 describes a mineral absorption promoter obtained by alcoholic fermentation and lactic acid fermentation of extracts or juices of wild grasses (nindo), vegetables, fruits, mushrooms and seaweeds. . However, even in this patent document, the effect of fermenting the gentian is not described other than the mineral absorption promoting effect, and the cell differentiation promoting effect, cell aging inhibiting effect, SA-β-Gal activity inhibiting disclosed in the present invention There is no suggestion about the effect and the melanin production inhibitory effect.

As an example of use of fermented gold and silver flowers in Patent Document 4, as a method for producing fermented herbal medicines such as gold and silver flowers, microorganisms such as filamentous fungi and yeast are added and subjected to primary fermentation for 3 to 8 days. Thereafter, a production method is described in which a microorganism such as Lactobacillus plantarum is inoculated and subjected to secondary fermentation for 6 to 12 days. By this two-stage fermentation, the fermentation period can be shortened, and further, the growth of rot and pathogenic microorganisms can be suppressed, and the addition of plant fermented products to foods can provide storage stability and increase the bioavailability. It is described that the flavor can be improved. However, in this document, in the primary fermentation, two types of microbial fermentation are performed by performing a process of fermenting with a fungus such as a filamentous fungus or a yeast, and a secondary fermentation with a bacterium such as a lactic acid bacterium. The purpose is to suppress the growth of other microorganisms by staged fermentation and to improve the flavor of food. For this reason, no mention is made of what kind of effect herbal medicines such as gold and silver flowers have on the skin function by fermentation treatment.

Patent Document 5 describes that yeast is cultured in a medium to which a gold and silver flower extract is added, and the cultured cell extract is used as an antioxidant. However, the technique disclosed in this document is a fermented gold and silver flower using yeast and is not a fermented product of lactic acid bacteria disclosed in the present invention.
Although yeast and lactic acid bacteria are the same microorganisms, yeast is a eukaryotic organism with a nucleus, and lactic acid bacteria are prokaryotes that do not have a nucleus. In addition, yeasts are abundant in sap, nectar, fruits, etc. in the natural world, and since they decompose sugars to produce alcohol and carbon dioxide, various foods such as bread, beer, wine, whiskey and miso have been used since ancient times. Has been used in the manufacture of On the other hand, since lactic acid bacteria decompose saccharides to produce lactic acid, fermenting vegetable materials such as vegetables, beans, rice, and wheat will change the taste and aroma of these materials, and the produced lactic acid will It has been used to improve the storage stability by suppressing the growth of microorganisms. Thus, yeast and lactic acid bacteria are greatly different in components that are metabolized even if the same saccharide is decomposed. Therefore, even if both Patent Document 5 and the present application are regarded as fermented products of gold and silver flowers by microorganisms, when gold and silver flowers are fermented with lactic acid bacteria, they are greatly different from substances produced by fermentation of yeast. It is thought that.

As described above, there is no example of cosmetics characterized by blending fermented products obtained by fermenting honeysuckle plants with lactic acid bacteria, and cell differentiation by treating honeysuckle plants with lactic acid bacteria. It has not been known that the promoting effect, the barrier function improving effect, the cell aging inhibiting effect, the SA-β-Gal activity inhibiting effect, and the melanin production inhibiting effect are enhanced.

JP 2004-073004 A JP 2004-208536 A JP2012-012363 Special table 2011-529702 JP2012-214392A

The object of the present invention is to provide various agents with no side effects and excellent safety, not a temporary moisturizing effect, but to improve the epidermal barrier function to form a healthy epidermis, and the dermal fibroblasts It is to provide a cosmetic material that forms a skin with elasticity and elasticity by suppressing aging and has a whitening effect.
In addition, it is an object to provide an active ingredient that can solve a plurality of skin problems at once with one active ingredient.

In light of the problems of the prior art, the present inventor has conducted extensive research on methods for solving these problems, and as a result, the cell differentiation promoting effect and barrier function strong against fermented products obtained by fermenting gold and silver flowers with lactic acid bacteria. It has been found that it has an improving effect, a cell aging inhibitory effect, an SA-β-Gal activity inhibitory effect, and a melanin production inhibitory effect, and the present invention has been completed.

Fermented material obtained by fermenting gold and silver flowers with lactic acid bacteria has stronger cell differentiation promoting effect, barrier function improving effect, cell aging inhibitory effect, SA-β-Gal activity inhibitory than gold and silver flower extract before fermentation treatment It was proved to have an effect and an inhibitory action on melanin production. However, these actions and effects were weak in the extract that was not subjected to fermentation treatment, and it was considered that the components in the plant were changed by fermentation treatment with lactic acid bacteria, and the effect was enhanced.

The evaluation criteria of cell differentiation are shown.

Hereinafter, the present invention will be described in detail.
First, an outline of the present invention will be described.
It is roughly divided into the following steps.
(First step) A step of obtaining a leachate in which gold and silver flowers are leached.
(Second step) A step of inoculating a lactic acid bacterium previously cultured in the leachate obtained in the first step and culturing it under a certain condition to obtain a fermented product.
(Third step) A step of dividing the fermented product obtained in the second step into a fermentation broth and a residue by filtration or the like.
(4th process) The process of adding a solvent etc. to the residue obtained at the 3rd process, and obtaining a fermentation extract.
In this application, the effective substance obtained by the 3rd process and the 4th process is utilized.

The terms in the present invention are defined as follows.
(1) The leaching solution refers to a product obtained by leaching a gold-silver flower used in the present application with a solvent such as purified water or ethanol, and indicates a stage before the gold-silver flower is removed by filtration or the like. So-called plant extracts that are appropriately heated are also included.
(2) The pre-culture extract refers to an extract obtained by removing gold and silver flowers from a leachate by filtration or the like.
(3) The fermented product refers to a product obtained by inoculating lactic acid bacteria in a leachate and culturing for a certain period.
(4) The fermentation broth refers to a product obtained by removing the floret portion fermented by filtration or the like from the fermented product.
(5) The fermented extract is obtained by adding an extraction solvent such as purified water to the fermented gold and silver flowers removed in (4), extracting under certain conditions, and then removing the residue by filtration or the like. Point to.
(6) The fermented product is a general term that combines a fermentation broth and a fermentation extract.
In the present application, a comparative experiment is performed when the lactic acid bacterium is treated later and the yeast is treated. However, in order to distinguish between the two, the “fermented product” is referred to as the “lactic acid bacterium fermented product” and the “fermentation culture solution”. Are sometimes synonymous with “lactic acid bacteria fermentation broth” and “fermentation extract” as “lactic acid bacteria fermentation extract”.
In addition, when performing the treatment defined in (1) to (6) above, when yeast is used instead of lactic acid bacteria, “yeast fermentation processed product”, “yeast fermentation culture solution”, “yeast fermentation extract” "," Yeast fermented product ". In this case, “yeast” is sometimes referred to as “yeast”, but it is synonymous.

The gold and silver flowers used in the first step of the present invention utilize the flower buds or flower parts of the honeysuckle genus Lonicera japonica Thunb. A flower bud part or a flower part refers to the whole part called a flower bud or a flower as a general concept, and points to a tip part from the flower axis. However, other parts may be mixed for convenience of the collection process.

The solvent for obtaining the leachate in the first step of the present invention is water, lower alcohols (methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, etc.) or these Can be used. Moreover, it is preferable to add sugar sources, nutrients, trace growth factors, and the like necessary for the growth of lactic acid bacteria as needed. The mixing ratio (weight ratio) between the gold and silver flowers and the leachate is generally 1: 1 to 1: 1000, preferably 1: 5 to 1: 100, more preferably 1:10 to 1:50 in terms of dry weight of the gold and silver flowers. Range.

The leachate in the first step of the present invention is obtained as follows.
First, gold and silver flowers to be leached are immersed or dispersed in the solvent. In this case, the gold and silver flowers may be used as they are, or may be used after being dried or semi-dried in advance. In addition, as the shape, the collected one can be used as it is, but if it is shredded or pulverized and refined, the fermentation efficiency can be increased in the subsequent fermentation process. In this invention, the said leaching solution is provided to the fermentation process which is the next process, without removing the leached gold and silver flowers. By inoculating and fermenting lactic acid bacteria, the tissue of gold and silver flowers can be decomposed by lactic acid bacteria.
This makes it possible to extract components that could not be extracted by the extraction by the conventional method, and an unprecedented effect can be obtained. However, in the case where the leaching is sufficiently performed, the components can be sufficiently extracted from the gold and silver flowers, so that the gold and silver flowers can be removed and used. In this case, the extracted component is fermented by lactic acid bacteria and converted into a new active ingredient. In that sense, even in a state corresponding to a normal plant extract, it can be used as a leachate.

The leachate from which the gold and silver flowers are leached can be used for the fermentation process as it is. It is sufficient that the gold and silver flowers are leached in a solvent, and no leaching time is required. However, a certain leaching time may be provided as long as the gold and silver flowers do not rot. Before the leachate is subjected to a fermentation process, it is preferable to sterilize and remove miscellaneous bacteria that hinder fermentation. In this case, as a method for removing germs, gold and silver flowers to be subjected to fermentation may be preliminarily washed and sterilized with sterilizing ethanol or the like, and then leached by a method of suspending in a sterile medium such as sterile water. After leaching and obtaining a leachate, a method of heat sterilization may be used.
As the heat sterilization method, an autoclave sterilization method in which a fermented liquid added with gold and silver flowers is heated at 105 to 121 ° C. for 10 to 20 minutes, or a fermented liquid added with gold and silver flowers is held at 80 to 90 ° C. for 60 to 120 minutes. The intermittent sterilization method is generally used once a day for 2-3 days. However, this sterilization process is not an essential process. If it does not become an obstacle in the next fermentation process, this sterilization process may be omitted.

The bacterium used in the fermentation process which is the second process of the present invention is a lactic acid bacterium. The lactic acid bacteria used in the present invention are not particularly limited as long as they belong to lactic acid bacteria. It can be easily separated from commercially available yogurt or from nature. Moreover, since it is also distributed in the microorganism distribution agency, it is possible to purchase and use the strain. Separation can be performed by a method described in JP 2012-105639 A or the like.

  Specifically, for example, lactic acid bacteria belonging to the genus Lactobacillus such as Lactobacillus plantarum, L. delbrueckii, L. brevis, L. casei, etc .; Lactic acid bacteria of the genus Leuconostoc, such as Leuconostoc mesenteroides, Leuconostoc citreum; Streptococcus faecalis, Streptococcus pyogenes, Streptococcus pyogenes; Enterococcus caseliflavus), Enterococcus sulfreus and other Enterococcus lactic acid bacteria; Lactococcus plantarum (Lactococcus plantar) um), Lactococcus rafinolactis and other Lactococcus lactic acid bacteria; Pediococcus damnosus, Pediococcus pentosaceus and other Pediococcus genus . Among these lactic acid bacteria, Lactobacillus plantarum (Lactobacillus plantarum) and Lactobacillus delbrueckii (L. delbrueckii) are the most used from the viewpoint of the effectiveness of the obtained fermented product and the fact that it is not extremely anaerobic and easy to handle. preferable. Since Lactobacillus delbrueckii is a typical lactic acid bacterium used for the production of yogurt, it can be easily separated from commercially available yogurt and from the natural world. Lactobacillus plantarum is said to be a plant lactic acid bacterium and is abundant in pickles and can be easily isolated.

As a culture medium for lactic acid bacteria, an MRS medium developed as a medium showing good growth of the entire lactic acid bacteria can be used. (MRS medium composition: peptone 10 g, beef extract 10 g, yeast extract 5 g, glucose 20 g, Tween 80 1 g, K ₂ HPO ₄ 2 g, sodium acetate 5 g, diammonium citrate 2 g, MgSO ₄ .7H ₂ O 0.2 g, MnSO ₄・ NH ₂ O 0.05g, purified water 1L)

As a medium composition of the lactic acid bacteria synthetic medium, it is sufficient that the minimum carbon source, nitrogen source and phosphorus source are included. Examples of the carbon source include ribose, arabinose, xylose, glucose, fructose, mannose, galactose, rhamnose, etc. Monosaccharides, sucrose, maltose, lactose, trehalose, cellobiose and the like, and trisaccharides such as raffinose and maltotriose can be used. One or more of these may be contained. Nitrogen sources include urea, potassium nitrate as nitrate, sodium nitrate, calcium nitrate, ammonium salts as ammonium sulfate and ammonium chloride, and amino acids as tryptophan, lysine, methionine, phenylalanine, threonine, valine, isoleucine, leucine, histidine, alanine, Nitrogen-containing compounds such as arginine, asparagine, serine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, proline, and tyrosine can be used, and one or more of these may be contained. As the phosphorus source, phosphate can be used, and potassium monohydrogen phosphate, potassium dihydrogen phosphate, and ammonium phosphate can be used, and one or more of these may be contained.

The basic medium of the synthetic medium is sufficient if it contains the carbon source, nitrogen source and phosphorus source. However, since lactic acid bacteria have strict auxotrophy, vitamin sources and mineral sources may be added. is necessary. Examples of vitamin sources include biotin, thiamine (vitamin B1), riboflavin, pyridoxine (vitamin B6), pantothenic acid, ascorbic acid, iodic acid, cyanocobalamin, inositol, nicotinic acid, choline, carnitine, paraaminobenzoic acid, nicotinamide adenidine Nucleotides and the like can be used. Examples of mineral sources include potassium, calcium, magnesium, sulfur, iron, manganese, zinc, boron, copper, molybdenum, sodium, iodine, cobalt, and the like. Specific components that can supply these include, for example, calcium nitrate. , Potassium chloride, calcium chloride, magnesium sulfate, sodium sulfate, ferrous sulfate, ferric sulfate, manganese sulfate, zinc sulfate, boric acid, copper sulfate, sodium molybdate, molybdenum trioxide, potassium iodide, cobalt chloride, etc. These compounds can be used.

In the present invention, the above components are appropriately combined to form a basic medium. Of course, it is also possible to culture in a synthetic medium containing no natural components and in a medium in which a small amount of natural products are combined when the growth of microorganisms is poor.

Of course, the medium composition other than the above can be applied to the present invention as long as it is a substance capable of assimilating and growing lactic acid bacteria. Furthermore, a mixed solution of lower alcohols (methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, etc.) or the like may be added to the medium to form a basic medium.

In the present invention, the lactic acid bacteria are stored in a dry state or a frozen state, and are cultured in advance in the basic medium or the like at the time of use. The culture at this stage may be performed as usual as long as each bacterium can grow.

The amount of inoculation in the inoculation step of lactic acid bacteria in the second step of the present invention is an appropriate amount of 10 ⁸ to 10 ¹⁰ cells / mL with respect to the leachate. Even if the inoculation amount exceeds the above range, the fermentation progress time hardly changes. On the other hand, if the inoculation amount is less than the above range, it takes a long time to complete the fermentation.

The fermentation temperature is generally in the range of 5 to 50 ° C, preferably in the range of 25 to 40 ° C, which is the optimum temperature for growth of lactic acid bacteria. The number of days of fermentation is generally in the range of 3 to 50 days, preferably 7 to 35 days, at the optimum temperature. When fermentation days become shorter than said general range, fermentation may not fully be performed but the effectiveness of fermented material may fall. On the other hand, even if it is longer than 35 days, the effectiveness beyond that is not recognized, which is not preferable. It is sufficient that the fermentation process is carried out by standing, but for shortening the fermentation time, it is also possible to carry out shaking culture and aeration culture.
The leachate which passed through the fermentation process on the said conditions is called a fermentation processed material. At this stage, the solvent and the plant are mixed. When the above fermentation process is completed, in order to stop the fermentation, the fermented product is subjected to a heat sterilization process at 80 to 120 ° C. for about 15 to 120 minutes.

In the third step, the fermented product after the sterilization treatment is used as it is, or generally and preferably by separating the liquid phase by solid-liquid separation means such as filtration or centrifugation to obtain a fermentation broth. The fermentation broth can be used as it is, but if necessary, it is diluted or concentrated to an appropriate concentration and used as a cosmetic raw material. In some cases, the liquid phase after solid-liquid separation is solidified according to a conventional method such as spray-drying method or freeze-drying method, and further pulverized as necessary and blended into a cosmetic. Also good.

In the fourth step, gold or silver flowers separated in the third step are mixed with water, lower alcohol (ethanol, propanol), glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, etc.) or a mixture thereof. Is used as an extraction solvent to obtain a fermentation extract. In addition, although the extraction temperature and extraction time at the time of obtaining a fermented extract can be suitably adjusted according to the objective, extraction temperature is also 80-120 degreeC also serving as a disinfection process, Preferably it is 90 to 105 degreeC. . The extraction time is 15 to 120 minutes, preferably 30 to 60 minutes.

The obtained fermented product can be used as a cell differentiation promoter, a barrier function improving agent, an SA-β-Gal activity inhibitor, and a melanin production inhibitor. Cosmetics containing fermented products include basic cosmetics such as emulsions, creams, lotions, essences, packs, facial cleansers, and makeup makeup such as lipsticks, foundations, liquid foundations, and makeup pressed powders. And cosmetics such as cosmetics, facial cleansers, body shampoos, soaps and the like, as well as bath agents, but of course are not limited thereto.

The blending amount of the fermented product in the cosmetic of the present invention is in the range of 0.0001 to 10% by mass, preferably 0.01 to 1% by mass in terms of the evaporation residue of the obtained culture solution. .

In addition to the above-mentioned essential ingredients, the cosmetics of the present invention include compounding ingredients usually used in cosmetics such as oily ingredients, surfactants, moisturizers, thickeners, antiseptic / disinfectants, powder ingredients, ultraviolet rays. Absorbers, pigments, fragrances and the like can be appropriately blended as necessary.

Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty acid amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, etc. ON surfactant: quaternary ammonium salt, primary to tertiary fatty acid amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

Examples of the humectant include glycols such as glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sugars such as maltitol, sorbitol, xylitol, trehalose, glucose, sodium pyrrolidonecarboxylate, mucopoly Sugars (eg, hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, hydrolyzed silk protein, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, Examples include phytosterol, soybean phospholipid, cholesteryl isostearate, seaweed extract, various amino acids, and derivatives thereof.

Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, and fartheraran, green algae or red algae-derived components, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, gums such as xanthan gum, tragacanth gum, guar gum and the like , Cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose, synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, glucosyl trehalose And sugar compounds mainly composed of hydrolyzed hydrogenated starch.

Examples of the antiseptic / bactericidal agent include paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride , Salicylic acid, ethanol, undecylenic acid, phenols, jarmal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark distillate, propolis extract and the like.

Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, 6- or 12-nylon powder, polyethylene powder. , Silk powder, cellulosic powder and the like.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Furthermore, if necessary, other active ingredients (whitening agents, skin aging prevention / skin roughening agents, anti-inflammatory agents, etc.) may be blended as long as the effects and features of the fermented product used in the present invention are not impaired. Examples of such a whitening agent include tranexamic acid and its derivatives, t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone derivatives, ellagic acid and its derivatives, resorcinol derivatives, placental extraction , Cysteine, Sakuhakuhi extract, Yukinoshita extract, Hamamelis extract, Itadori extract, Licorice extract, Gentian extract, Jujube extract, Peonies extract, Sugi extract, Peach extract, Green algae, Red algae or Brown algae Seaweed extracts, linoleic acid and its derivatives or processed products (eg linoleic acid ment Lester, etc.), 2,5-dihydroxybenzoic acid derivatives, etc. are components for preventing skin aging and improving rough skin, animal or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, intercellular lipids such as ceramide, placenta extraction Nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A precursor, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin, α-hydroxy acids, diisopropyl Amine dichloroacetate, γ-amino-β-hydroxybutyric acid, coenzyme Q-10, adenosine, α-lipoic acid, picoline, carnitine and its derivatives, gentian extract, licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract, etc. Herbal medicine extraction Kisses, green algae, red algae or brown algae seaweed extract, Sakuhakuhi extract, beech extract, beetle aloe extract, mannenrou extract, ginkgo biloba extract, horsetail extract, safflower extract, rapeseed extract, elderberry extract For example, guaiazulene sulfonic acid can be used as an anti-inflammatory component, for example, Guagomogusa extract, astragalus extract, mango extract, eggshell membrane extract protein, deoxyribonucleic acid potassium salt, purple orchid root extract, purple plant extract, rice extract, etc. Azulene derivatives such as sodium and ethyl guaiazulenesulfonate, glycyrrhizic acid derivatives such as dipotassium glycyrrhizinate and stearyl glycyrrhetinate, allantoin, licorice extract, cucumber extract, peonies extract, button pi extract, forsythia extract, ryutan Distillate, toe calendula extract, parsley extract, Hypericum extract, and the like. Antioxidants include, for example, superoxide dismutase, bioactive oxygen-degrading enzymes such as catalase, vitamins such as vitamin E and vitamin D and derivatives thereof, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, There are ubidecaquinone (ubiquinone), rutin, rutin glucoside, γ-oryzanol and the like.

Examples of fermented product effect tests in the present invention will be described below. Furthermore, although the application formulation example etc. to the skin composition using fermented material are described, it is not limited to the Example described here. In the following, all parts are parts by mass, and all% are% by mass.

Preparation of lactic acid bacteria to be inoculated into gold and silver flower leachate Lactobacillus plantarum (NBRC 101975), which was sold by the National Institute for Product Evaluation Technology, was used as lactic acid bacteria. Lactic acid bacteria were cultured in MRS medium, 30 ml of the medium was inoculated with 1 platinum loop of the strain that had been transferred, and statically cultured at 30 ° C. for 3 days to obtain a lactic acid bacteria culture solution. (MRS medium composition: peptone 10 g, beef extract 10 g, yeast extract 5 g, glucose 20 g, Tween 80 1 g, K ₂ HPO ₄ 2 g, sodium acetate 5 g, diammonium citrate 2 g, MgSO ₄ .7H ₂ O 0.2 g, MnSO ₄・ NH ₂ O 0.05g, purified water 1L)

Preparation of fermented product 100 ml of purified water was added to 10 g of dried gold and silver flowers and leached at room temperature. Thereafter, sterilization was performed at 105 ° C. for 15 minutes to obtain a leachate. After cooling, 5 ml of the lactic acid bacteria culture solution was added to the leachate and stirred well. The exudate in which lactic acid bacteria were planted was subjected to stationary culture at 30 ° C. for 28 days to obtain a fermented gold and silver flower.

Preparation of fermented gold and silver flower The fermented product obtained was sterilized at 105 ° C. for 15 minutes in order to stop the fermentation process. After sterilization, the culture solution was squeezed out with a filter cloth. The filtrate was centrifuged at 6,000 rpm × 10 min., And the supernatant was taken as a gold and silver flower fermentation broth for effect test. Further, purified water was added at a rate of 10 ml to 1 g of gold and silver flower residue remaining on the filter cloth, and sterilized and extracted at 105 ° C. for 15 minutes. After sterilization extraction, the extract was squeezed out with a filter cloth to obtain a gold and silver flower fermentation extract for effect test. Further, as a negative control, a culture solution (“plant-free / fermented culture solution”) was prepared after culturing only with lactic acid bacteria [0045] to which no gold-silver flowers were added, followed by centrifugation.

<Cell differentiation promoting effect and barrier function improving effect>
In skin with an abnormal barrier function, the stratum corneum barrier is incomplete, so that irritants and the like easily enter the skin, and inflammation is likely to occur. In such inflamed skin, keratinocytes reach the stratum corneum in an undifferentiated state, and a normal stratum corneum is not formed. For this reason, skin inflammation is likely to occur, and this inflammation causes a vicious cycle in which the barrier function becomes abnormal. Therefore, if cell differentiation can be promoted, it is considered that the function of healthy epidermal cells is restored and an effect of improving the barrier function can be expected. Then, the cell differentiation promotion effect of the lactic acid bacteria fermentation metabolite of the gold and silver flower was confirmed.

Cell culture cells: Hacat cell culture medium: Humedia KG2
D-MEM (Ca concentration: 1.8 mM)
Fixing solution: Mildform 10NM (Wako Pure Chemical Industries)
Staining: 0.05% naphthol blue black solution (9% acetic acid, 0.1M sodium acetate)
Hacat cells that are human epidermal cells were cultured in Humedia KG2 (Kurabo) medium.
Cells were planted in a 12-well plate to about 50% confluence and cultured. The next day, each sample was added. After the addition, the cells were fixed after culturing for 72 hours, stained with a naphthol blue black solution, the morphology of the cells was observed with a microscope, and the degree of differentiation was determined.

Evaluation of cell differentiation When keratinocytes, which are epidermal cells, differentiate, the cells adhere to each other and take an amorphous form. On the other hand, undifferentiated cells are independent of each other and do not adhere. Differentiation of keratinocytes is promoted by the Ca concentration in the medium. As shown in the photograph in [Fig.1], when Hacat cells are cultured in a D-MEM medium with a Ca concentration of 1.8 mM, the cells are completely differentiated (corresponding to the photograph with the evaluation point 5), and the Humedia KG2 medium with a low Ca concentration Then, the cells proliferate in an undifferentiated state (corresponding to the photograph with the evaluation score 1). The degree of adhesion between undifferentiated and differentiated cells was visually evaluated with a microscope in five stages to evaluate the degree of cell differentiation. The criteria for cell differentiation were determined according to the criteria shown in [Fig. 1].

[Table-1] shows the results of the cell differentiation degree when each sample was added based on the evaluation criteria of the cell differentiation degree evaluation in [Fig.-1]. The experiment was performed by adding 2.5% of the fermented product added to the cells to the medium prepared in [0046]. As a comparative example-3, the results of a gold-silver flower / fermented culture solution as an example-1, a gold-silver flower / fermented culture solution as example-1, and a gold-silver flower / fermented extract as example-2 are shown. The gold and silver flower / pre-culture extract of Comparative Example-3 showed a cell differentiation degree rating of 2 and showed almost no cell differentiation promoting effect. However, the gold / silver flower / fermentation broth of Example-1 has a cell differentiation score of 5 points, and as Example-2, the gold / silver flower / fermentation extract also has a cell differentiation score of 5, indicating a very high cell differentiation promotion. Showed the effect. Further, Comparative Example-4, which was tested as a negative control, had a cell differentiation score of 1, indicating that the lactic acid bacteria culture solution alone had no cell differentiation promoting effect. In addition, the cell differentiation degree score of the undifferentiated cells cultured in the Humedia KG2 medium of Comparative Example-2 was determined as 1 point, and the cell differentiation degree score of the differentiated cells cultured in the D-MEM medium was determined as 5 points. From the above results, it was found that the components extracted from gold and silver flowers were changed by fermentation treatment with lactic acid bacteria, and the cell differentiation promoting effect was enhanced.

<SA-β-Gal activity inhibitory effect / cell aging improvement effect>
Decrease in skin functions such as elasticity, elasticity and sagging that progress with aging is caused by aging at the cellular level. In other words, aged fibroblasts have decreased production of hyaluronic acid and collagen, resulting in loss of elasticity and elasticity of the dermis. On the other hand, in human skin fibroblasts, it is known that SA-β-Gal activity (senescence-associated beta-galactosidase) in cells increases with the passage of cells and the age of collected humans. -β-Gal activity has been used as a cellular senescence marker. Therefore, if the SA-β-Gal activity can be suppressed, a cell aging inhibitory effect can be expected, and skin firmness / elasticity and sagging can be improved. Therefore, SA-β-Gal activity of lactic acid bacteria fermentation metabolites of gold and silver flowers was confirmed.

Measurement of cell aging by measuring SA-β-Gal activity Cultured cells: Normal human fibroblasts NB1-RGB (RIKEN)
Medium: D-MEM FBS 10%
Normal human fibroblasts NB1-RGB were cultured, and 200 μM hydrogen peroxide was added to the cell culture medium for 2 hours a day. This was repeated for 2 days in succession to cause cell senescence. After adding the sample prepared in [0046] to the cell culture medium at a concentration of 2.5% for 3 days, SA-β-Gal staining was performed, and the degree of cell aging was measured based on the degree of cell staining. Note that nicotinamide was used as a positive subject.
Measurement of SA-β-Gal activity Fix cells with fixative for 5 minutes. After washing the cells with PBS (−), an SA-β-Gal solution was added and left at 37 ° C. for 16 hours.
Cells stained with the SA-β-Gal staining solution were collected, dissolved in 2N-NaOH solution, the absorbance at 655 nm was measured (A value), and SA-β-Gal activity was measured. A part of the lysate was measured for absorbance at 540 nm (B value) by the BCA method, and the amount of protein was measured. The SA-β-Gal activity per cell protein amount was determined by the following calculation formula.

SA-β-Gal activity = absorbance value at 655 nm (A value) / absorbance value at 540 nm measured by the BCA method (B value)

Fixing solution: 95 mL of PBS (−) is added to 5 mL of formaldehyde solution (reagent formalin 37% concentration).
X-Gal solution: 20 mg of X-Gal (Wako Pure Chemical Industries) is dissolved in 1 mL of DMSO.
SA-β-Gal staining solution: Dissolve in the following staining buffer so that X-Gal has a concentration of 1 mg / mL.
Staining Buffer: 40 mM citric acid: 40 mM disodium hydrogen phosphate (1: 3)
Dissolve the following 4 reagents in the aqueous solution adjusted to pH 6.0.
5 mM potassium ferrocyanide
5 mM potassium ferricyanide 150 mM NaCl
2 mM MgCl ₂
BCA method: The following C liquid and D liquid are mixed at a ratio of 50: 1 immediately before measurement.
To 40 μL of cell lysate dissolved in 2N-NaOH, 200 μL of a mixture of solution C and solution D is added and reacted at 37 ° C. for 30 minutes, and then the absorbance at 540 nm is measured to determine the amount of protein.
Liquid C Sodium bicinchoninate 10g
Na ₂ CO ₃ 20g
Sodium tartrate 1.6g
NaOH 4g
9.5 g of NaHCO ₃
After dissolving with purified water, the pH is adjusted to 11.25 with 10N NaOH, and then adjusted to 1L.
D solution CuSO ₄ · 5H ₂ O 4g
Make up to 100 mL with purified water.

[Table-2] shows the results of cell aging degree by measuring SA-β-Gal activity. SA-β-Gal activity score was 13.8 in cells that were treated twice for 2 hours at a concentration of 200 μM hydrogen peroxide in Comparative Example-5. On the other hand, SA-β-Gal activity score was 5.36 in the cell of Comparative Example-6 which was not treated with hydrogen peroxide. In addition, in the cells cultured in hydrogen peroxide-treated 200 μM concentration in Comparative Example 7 twice for 2 hours and then added with 5 mM nicotinamide, the SA-β-Gal activity score is 5.63. It was found that SA-β-Gal activity was suppressed by the addition of 5 mM nicotinamide. Furthermore, in Comparative Example-8 to which the gold-silver flower / pre-culture extract was added, the SA-β-Gal activity score was 4.85. In addition, Comparative Example 9 tested as a negative control had an SA-β-Gal activity score of 13.3, indicating that there was no cell aging inhibitory effect only with the culture solution of lactic acid bacteria. However, SA-β-Gal activity score was 3.19 in the gold / silver flower / fermentation culture solution addition section of Example-3, and SA-β-Gal activity score of the gold / silver flower / fermentation extract addition section was Example-4. Also showed a very high cell aging inhibitory effect. From this result, it was found that the components extracted from gold and silver flowers were changed by fermentation treatment with lactic acid bacteria, and the effect of inhibiting cell aging was enhanced.
Therefore, it can be said that the SA-β-Gal activity inhibitor of the present invention can also be used as a cell aging inhibitor.

<Whitening effect>
Culture of melanin production inhibition test cells Mouse melanoma B-16 / F-10 cells are dispersed in D-MEM (Invitrogen) medium containing 5% fetal calf serum and seeded at 5 × 10 ⁴ / well on a 12-well plate. And culture at 37 ° C. for 1 day. Add the medium to 1 ml per well and culture. After culturing for one day, the sample prepared in [0046] was added to the cell culture medium at a concentration of 2.5%, and an arbutin aqueous solution as a positive target was added as shown in [Table-3]. The culture was performed for a day.
Evaluation Method The amount of melanin produced in the cells was measured after culturing, by dissolving the cells in 2N-NaOH and measuring the absorbance at 405 nm. In addition, the degree of cell proliferation was determined by measuring a part of the cell lysate dissolved in 2N-NaOH at an absorbance of 540 nm by the protein measurement method by the BCA method, and converting it to the amount of protein. The degree of melanin production was calculated by the ratio of the amount of melanin per unit protein amount.

Calculation formula: Melanin production rate (%) = (absorbance value at 405 nm in the sample addition group / absorbance value at 540 nm in the sample addition group) / (absorbance value at 405 nm in the no addition group / absorbance value at 540 nm in the no addition group) × 100

Table 3 shows the results of the melanin production inhibitory effect of gold and silver flowers and fermented products.
In Comparative Example-12 to which the gold-silver flower / pre-culture extract was added, the melanin production was 90.4, but in the case of adding the gold-silver flower fermentation broth of Example-5, the melanin production was 76.8. In the case of adding the gold and silver flower fermentation extract of Example-6, the melanin production degree was 72.9, and it was confirmed that the melanin production was suppressed by the fermentation treatment. In addition, when arbutin was added at 50 ppm in Comparative Example-10 measured as a positive control, the melanin production was 81.9, and when Comparative Example-11 was added at 100 ppm, it was 75.4. Furthermore, in Comparative Example-13 tested as a negative control, the degree of melanin production was 97.6, indicating that the lactic acid bacteria culture solution alone has no melanin production inhibitory effect. From this result, it turned out that the component extracted from a gold-silver flower changed by the fermentation process by lactic acid bacteria, and the melanin production suppression effect increased.

As human test subjects, 10 females aged 20 to 65 years were allowed to use the skin preparations for the test and comparative products twice a day (morning and evening) for 1 month in succession. The state was compared before and after the test, and the improvement effect was investigated. In this test, the external preparation for skin shown in Formulation Example 7 was used as a test product (Example 7), and a fermented product (gold and silver flower fermentation broth and fermented broth from the external preparation for skin shown in Formulation Example 7 was used as a comparative product. The skin external preparation replaced with purified water was prepared (Comparative Example 14), and the effect of its use was investigated. Evaluation was performed for each item according to the criteria shown in Tables 4, 5, and 6. The skin condition was totaled in questionnaires before the start of application and after one month of application while daily use of the external preparation for skin containing the active ingredient of the present invention, and the effect was confirmed. The results are shown in Tables 7, 8, and 9. The numbers in the table indicate the number of people. As shown in Table-7, in the skin external preparation of the test product containing the fermented product, the skin elasticity evaluation score was 81 points. On the other hand, in the comparative product obtained by removing the fermented product from the test product, the evaluation score was 36 points. As is apparent from this result, it was revealed that the elasticity of the skin is increased by adding the fermented product. Moreover, as shown in Table-8, the skin external preparation of the test product containing the fermented product showed a score of 83 for moisturizing the skin. On the other hand, in the comparative product obtained by removing the fermented product from the test product, the evaluation score was 39 points. As is clear from this result, it became clear that the addition of the fermented product increased the moisture of the skin. Furthermore, as shown in Table-9, the skin external preparation of the test product containing the fermented product showed a skin dullness evaluation score of 85 points. On the other hand, in the comparative product obtained by removing the fermented product from the test product, the evaluation score was 38 points. As is clear from this result, it became clear that the dullness of the skin was reduced by adding the fermented product.

Various effects of the present invention are as described above, but in order to clarify the difference from the prior art, a confirmation test was also conducted for the case of using “yeast bacteria” instead of “lactic acid bacteria” of the present invention.
The melanin production inhibitory effect of the present invention is due to fermentation treatment with lactic acid bacteria, and is an effect that cannot be obtained when yeast is used, and is not due to an antioxidant effect. Show.

Preparation of Yeast Culture Solution Saccharomyces cerevisiae was used in a conical flask to which 50 ml of YM medium sterilized at 120 ° C. for 15 minutes was added using a strain (NBRC 0308) that was distributed from the National Institute for Product Evaluation Technology (Germany). A yeast culture solution was prepared by adding platinum ears and allowing them to stand and grow at 25 ° C. for 5 days.
<YM medium>
Peptone 5g / L, yeast extract 3g / L, malt extract 3g / L, glucose 10g / L

Preparation of Yeast Fermentation Processed Product The fermentation process product was prepared by adding 100 ml of purified water to 10 g of dried gold and silver flowers and leaching at room temperature in the same manner as the fermented product of lactic acid bacteria described in [0045]. Thereafter, sterilization was performed at 105 ° C. for 15 minutes to obtain a leachate. After cooling, 5 ml of the yeast culture solution described in [0067] was added to the leachate and stirred well. The leachate in which the yeast was planted was subjected to stationary culture at 25 ° C. for 28 days to obtain a fermented gold and silver yeast fermentation product.

Preparation of Fermented Product The gold and silver flower yeast fermented product obtained was sterilized at 105 ° C. for 15 minutes in order to stop the fermentation process. After sterilization, the culture solution was squeezed out with a filter cloth. The filtrate was centrifuged at 6,000 rpm × 10 min., And the supernatant was taken as a gold and silver flower yeast fermentation broth for effect test. Further, purified water was added at a rate of 10 ml to 1 g of gold and silver flower residue remaining on the filter cloth, and sterilized and extracted at 105 ° C. for 15 minutes. After sterilization extraction, the extract was squeezed out with a filter cloth to obtain a gold and silver flower yeast fermentation extract for effect test. In addition, as a comparative control plant, “Huang”, which was dried from the ears of the genus Gyceae, was treated with lactic acid bacteria and yeast in the same manner to prepare each fermented product.

DPPH radical scavenging activity test [0046] "0069" sample and 0 mM, 0.3 mM, 0.5 mM, 0.7 mM, 1.0 mM, 3.0 mM, 5.0 mM, 10 mM Trolox (6-hydroxy- To 10 μL of 2,5,7,8-tetramethylchroman-2-carboxylic acid) solution, 190 μL of DPPH solution prepared for use at the following ratio was added, and after 15 minutes, absorbance at 540 nm was measured to evaluate DPPH radical scavenging ability. A calibration curve was prepared from the absorbance of Trolox solution of known concentration, and the strength of DPPH radical scavenging ability of the sample was displayed as trolox equivalent.
Trolox solution: 25 mg of trolox was dissolved in 10 ml of DMSO (Dimethyl sulfoxide) to prepare a trolox 10 mM solution.
DPPH solution 0.4 mM-DPPH (1,1-Diphenyl-2-picrylhydrazyl) 40
400 mM MES buffer (2-Morpholinoethanesulfonic acid) (PH6.1) 10
Distilled water 30

Table 10 shows measured values of DPPH radical scavenging activity. The DPPH scavenging activity of the gold and silver flower pre-culture extract was 0.89 mM Trolox equivalent. As a result of the yeast fermentation treatment, the DPPH elimination activity of the gold and silver flower yeast fermentation broth became 0.94 mM Trolox equivalent, and the DPPH elimination activity of the gold and silver yeast yeast fermentation extract became 1.33 mM Trolox equivalent. However, depending on the fermentation process of lactic acid bacteria, 0.85 mM Trolox equivalent in the fermentation broth of gold and silver lactic acid bacteria and 0.27 mM Trolox equivalent in the fermentation extract of gold and silver lactic acid bacteria. Compared to the above, DPPH scavenging activity decreased to 1/5. As is clear from this result, it is clear that the DPPH elimination activity of the fermented product of each bacterium does not show the same tendency in the yeast and lactic acid bacterium even if the same gold and silver flowers are fermented. Furthermore, [Table-11] shows DPPH elimination activity of fermented products obtained by fermenting not only gold and silver flowers but also “Yellow”, a plant belonging to the family Gramaaceae, with lactic acid bacteria or yeast.
The DPPH elimination activity of the extract before koji yellow culture, the koji yellow yeast fermentation extract, and the koji lactic acid bacteria fermentation extract was 0.30 mM, 0.36 mM, and −0.381 mM Trolox equivalents, respectively.
The DPPH scavenging activity of the yellow lactic acid bacteria fermentation extract was -0.381 mM Trolox equivalent, and conversely promoted oxidation.
As illustrated above, it is clear that even if the microorganism is the same, the characteristics of the fermented material are different depending on each fungus and do not exhibit the same behavior.

The strength of DPPH scavenging activity is generally a measure of the strength of antioxidant activity, but does not necessarily coincide with the melanin production inhibitory effect.
As shown in [Table-3], the melanin production was 90.4 in Comparative Example-12 in which the extract before cultivating gold and silver flowers was added, but when the fermented bronze broth in Example-5 was added Then, the melanin production degree was 76.8, and when the gold and silver flower fermentation extract of Example-6 was added, the melanin production degree was 72.9, and it was confirmed that the melanin production was suppressed by the fermentation treatment. At this time, the DPPH elimination activity of the pre-cultured gold-silver flower lactic acid bacteria extract of [Table-10], the gold-silver flower lactic acid bacteria fermentation broth, and the gold-silver flower lactic acid bacteria fermentation extract was 0.89 mM, 0.85 mM, and 0.27 mM, respectively. And do not indicate related numbers. As is clear from this example, it is also clear that a strong antioxidant effect does not always suppress melanin production.

Next, although the formulation example which mix | blended the fermented material of this invention is shown, this invention is not limited to this.

Examples of cosmetic preparations The fermented products shown in the following cosmetic preparation examples show each culture solution and extract prepared by the method shown in [0046].
(Formulation Example 1) Cosmetic cream (mass%)
a) Beeswax 2.0
b) Stearyl alcohol ... 5.0
c) Stearic acid ... 8.0
d) Squalane ... 10.0
e) Self-emulsifying glyceryl monostearate 3.0
f) Polyoxyethylene cetyl ether (20E.O.) ... 1.0
g) Gold and silver flower fermentation broth ... 5.0
h) Gold and silver flower extract ... 5.0
i) 1,3-butylene glycol ... 5.0
j) Potassium hydroxide ... 0.3
k) Preservatives / Antioxidants: appropriate amount
l) Heat and dissolve purified water: remaining manufacturing methods a) to f) and keep at 80 ° C. Heat up to i) to l), keep at 80 ° C., add to a) to f), emulsify, and cool to 40 ° C. with stirring. Thereafter, g) and h) are added and stirred to dissolve uniformly.

Cosmetic Formulation Example (Formulation Example 2) Cosmetic Cream (mass%)
a) Beeswax 2.0
b) Stearyl alcohol ... 5.0
c) Stearic acid ... 8.0
d) Squalane ... 10.0
e) Self-emulsifying glyceryl monostearate 3.0
f) Polyoxyethylene cetyl ether (20E.O.) ... 1.0
g) Gold and silver flower fermentation broth ... 10.0
h) Gold and silver flower extract ... 5.0
i) 1,3-butylene glycol ... 5.0
j) Potassium hydroxide ... 0.3
k) Preservatives / Antioxidants: appropriate amount
l) Heat and dissolve purified water: remaining manufacturing methods a) to f) and keep at 80 ° C. Heat up to i) to l), keep at 80 ° C., add to a) to f), emulsify, and cool to 40 ° C. with stirring. Thereafter, g) and h) are added and stirred to dissolve uniformly.

(Prescription Example 3) Emulsion (mass%)
a) Beeswax 0.5
b) Petrolatum 2.0
c) Squalane ... 8.0
d) Sorbitan sesquioleate ... 0.8
e) Polyoxyethylene oleyl ether (20EO) 1.2
f) Gold and silver flower fermentation extract ... 0.1
g) Gold and silver flower fermentation broth ... 1.0
h) 1,3-butylene glycol 7.0
i) Carboxyvinyl polymer 0.2
j) Potassium hydroxide ... 0.1
k) Purified water: balance
l) Preservatives / Antioxidants: appropriate amount
m) Ethanol: 7.0
The production methods a) to e) are dissolved by heating and kept at 80 ° C. Heat up to h) to k), keep at 80 ° C., add to a) to e), emulsify, and cool to 50 ° C. with stirring. Add f), g), m) at 50 ° C., stir to 40 ° C. and cool.

(Formulation Example 4) Lotion (mass%)
a) Gold and silver flower extract ... 0.01
b) Gold and silver flower fermentation broth ... 0.001
c) Glycerin ... 5.0
d) Polyoxyethylene sorbitan monolaurate (20EO) ... 1.0
e) Ethanol ... 6.0
f) Perfume ... appropriate amount g) antiseptic / antioxidant ... appropriate amount h) purified water ... the remaining preparation methods a) to h) are mixed and dissolved uniformly.

(Formulation Example 5) Lotion (mass%)
a) Fermented bronze broth ... 0.001
b) Gold and silver flower fermentation extract ... 10.0
c) Glycerin ... 5.0
d) Polyoxyethylene sorbitan monolaurate (20E.O.) ... 1.0
e) Ethanol ... 6.0
f) Fragrance: appropriate amount
g) Preservatives / Antioxidants ...
h) Purified water: The remaining preparations a) to h) are mixed and dissolved uniformly.

(Formulation Example 6) Face wash (mass%)
a) Stearic acid ... 12.0
b) Myristic acid 14.0
c) Lauric acid ... 5.0
d) Jojoba oil ... 3.0
e) Glycerin 10.0
f) Sorbitol ... 15.0
g) 1,3-butylene glycol ... 10.0
h) POE (20) Glycerol monostearic acid 2.0
i) Potassium hydroxide ... 5.0
j) Water ... balance k) chelating agent ... appropriate amount l) perfume ... appropriate amount m) gold and silver flower fermentation extract ... 1.0
n) Gold-silver flower fermentation broth ... 1.0
The manufacturing methods a) to h) are dissolved by heating and maintained at 70 ° C. After i) is dissolved in j), it is added to a) to h) and saponified. Then, k) and l) are added and cooled with stirring. Add m), n) at 50 ° C., stir to 40 ° C. and cool.

(Prescription Example 7) Essence (mass%)
a) Gold and silver flower extract ... 2.0
b) Gold-silver flower fermentation broth ... 2.0
c) Hiboswako ... 0.05
d) L-arginine ... appropriate amount
e) Glycerin ... 5.0
f) Polyoxyethylene sorbitan monolaurate (20E.O.) ... 1.0
g) Ethanol ... 6.0
h) Fragrance: appropriate amount
i) Preservatives / Antioxidants ...
j) Purified water: The rest of the production method c) is dispersed in a part of j), and d) is added to adjust the pH to 6.5. Then, a) to j) are mixed and dissolved uniformly.

In the present invention, the leaching solvent and the extraction solvent used for the leaching solution, the extraction solution and the like are all purified water unless otherwise indicated, but the purified water is used regardless of the type of the extraction solvent. The same effect as that obtained was obtained.

As described in detail above, according to the present invention, a fermented product obtained by fermenting the flower part of gold and silver flowers with lactic acid bacteria on the skin where the elasticity of the skin is reduced and wrinkles and sagging are noticeable, or on the skin where the spots and dullness are increased. By applying a topical skin preparation formulated as an active ingredient, aging of fibroblasts is suppressed, and the elasticity of the skin is restored, wrinkles and sagging are reduced, and skin with no spots or dullness is restored. Became possible.

Claims (6)

A cell differentiation promoting agent comprising a fermented product obtained by fermenting gold and silver flowers with lactic acid bacteria as an active ingredient. A barrier function improving agent comprising a fermented product obtained by fermenting gold and silver flowers with lactic acid bacteria as an active ingredient. A SA-β-Gal activity inhibitor characterized by containing a fermented product obtained by fermenting gold and silver flowers with lactic acid bacteria as an active ingredient. A melanin production inhibitor comprising a fermented product obtained by fermenting gold and silver flowers with lactic acid bacteria as an active ingredient. The agent according to any one of claims 1 to 4, wherein lactic acid bacteria are obtained by fermentation with Lactobacillus plantarum. A cosmetic comprising a fermented product obtained by fermenting gold and silver flowers with lactic acid bacteria as an active ingredient.