JP2006188494A - External preparation or oral beautiful skin-promoting agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new use using Terminalia bellirica. <P>SOLUTION: The external preparation of the present invention contains a processed product obtained from Terminalia bellirica as an active ingredient. The processed product obtained from the Terminalia bellirica has an excellent active oxygen-eliminating ability, a tyrosinase-inhibiting action, and a lactate dehydrogenase-inhibiting action, and further has an action for preventing or improving stains, freckles, wrinkles and the like, an action for maintaining or improving the strength of mucous membranes, and an action for preventing periodontal diseases and dental caries. The oral beautiful skin-promoting agent of the present invention has the same actions as those of the external preparation of the present invention. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to an external preparation or an oral skin beautifying agent containing a processed product obtained from Terminaria berylica as an active ingredient.

  Terminalia bellirica is a plant of the family Sequimidae. For example, in India, the fruit is mainly used as an antidiarrheal agent and the ripe fruit is used as an antidiarrheal agent.

In recent years, it has been found that the fruit of Terminaria berylica has an action of preventing vascular fibrosis, and a prophylactic or therapeutic agent for vascular fibrosis comprising a dry powder or an extract thereof has been disclosed (Patent Literature). 1). However, the effects of terminaria have not yet been fully studied, and terminaria is not fully utilized at present.
JP 2004-75584 A

  The object of the present invention is to expand the use of terminaria by finding a new action of terminaria berylica.

  This inventor earnestly examined about the effect | action which Terminaria berylica has. As a result, Terminaria berylica has excellent active oxygen scavenging ability, tyrosinase inhibitory action, and lactate dehydrogenase inhibitory action required for beautifying the skin or preventing aging and diseases in the skin, oral cavity, etc. Found to have.

  The present invention provides an external preparation containing a processed product obtained from Terminaria berylica as an active ingredient.

  Furthermore, this invention provides the skin-care promoter for oral use which contains the processed material obtained from Terminaria berylica as an active ingredient.

  ADVANTAGE OF THE INVENTION According to this invention, the external preparation which can utilize the effect | action which Terminaria berylica has efficiently, and the skin-care promoter for oral use can be provided. As mentioned above, Terminaria berylica has excellent active oxygen scavenging ability, tyrosinase inhibitory action, and lactate dehydrogenase inhibitory action, so it has the effect of improving skin quality and the effect of preventing aging and diseases such as skin and oral cavity. Can demonstrate.

(Processed product obtained from Terminaria berylica)
A processed product (hereinafter, simply referred to as a processed product) obtained from Terminaria berylica used in the present invention is a part of a leaf, root, bark, flower, xylem, fruit, etc., dried, pulverized, or pressed. It is obtained by performing processing, extraction processing, and the like. Examples of such processed products include dried products, pastes, juices and extracts. In the present invention, any part of Terminaria berylica may be used, and particularly a part other than seeds is preferable, and a part excluding fruit seeds (fruit skin or flesh part) is more preferable.

  The dried product, which is an embodiment of the processed product, can be obtained, for example, by subjecting each part of Terminaria berylica to sun drying, half sun drying, shade drying, heat drying, room temperature drying, freeze drying, and the like. After drying, if necessary, it may be pulverized using a ball mill, a hammer mill or the like to obtain a dry powder.

  The paste which is the one aspect | mode of the said processed material is obtained, for example by grind | pulverizing each site | part of terminaria berylica with a mascolloider etc., without drying. Further, the juice is obtained by filtering or centrifuging the paste. Squeezed juice can also be obtained by squeezing each part of the above-mentioned Terminaria berylica. The obtained juice may be used as it is, or may be dried to obtain an extract powder.

  The extract which is one form of the processed product is obtained by, for example, extracting each part of the above-mentioned Terminaria berylica as it is from a paste or a dried product using water or an organic solvent. In particular, it is preferable to extract from paste or dry powder.

  Examples of the solvent that can be used to obtain the extract include water, an organic solvent, and a mixed solution of water and an organic solvent. Examples of the organic solvent include methanol, ethanol, n-propanol, n-butanol, acetone, Hexane, cyclohexane, propylene glycol, ethyl methyl ketone, glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible fats and oils, 1,1,1,2-tetrafluoroethane, 1,1,2-trichloroethene, etc. Can be mentioned. Of these, polar organic solvents are preferred, more preferably ethanol, n-butanol, methanol, acetone, propylene glycol, and ethyl acetate, most preferably ethanol.

  The amount of the solvent used for the extraction is not particularly limited as long as the component contained in Terminaria berylica is sufficiently extracted. For example, when obtaining an extract from the dried product of Terminaria berylica, the amount of the solvent is preferably 1 to 50 parts by mass with respect to 1 part by mass of the dried product. When the extract is obtained from other than the dried product, the amount of the solvent is preferably 0.1 to 30 parts by mass with respect to 1 part by mass of the dried product.

  The extraction temperature is not particularly limited, and can be appropriately set according to the boiling point of the solvent used. In order to extract efficiently, extraction temperature becomes like this. Preferably it is 10 degreeC or more, More preferably, it is 20 degreeC or more, Preferably it is 120 degrees C or less, More preferably, it is below the boiling point of the solvent to be used.

  The extraction time can be appropriately set depending on the amount of extraction solvent used or the extraction temperature. The extraction time is preferably 30 minutes or longer, more preferably 1 hour or longer, preferably 72 hours or shorter, more preferably 48 hours or shorter.

  In this way, an extract can be obtained from Terminaria berylica. The obtained extract may be used as an extract as it is, or may be concentrated by a method commonly used by those skilled in the art and used as an extract in the form of a paste or powder, if necessary. In order to concentrate the extract, heating to dryness, vacuum concentration to dryness, freeze drying, and the like are performed. Among these, from the viewpoint of suppressing the denaturation of the components in the extract, it is preferably vacuum-concentrated to dryness or freeze-dried.

  Further, in order to make the obtained extract more active, this extract was synthesized with a synthetic adsorbent (Diaion HP20, Sephabies SP825, Amberlite XAD4, MCIgelCHP20P, etc.) and dextran resin (Sephadex LH-20, etc.). ) Can be used to fractionate or concentrate the components.

  The processed product of Terminaria berylica thus obtained has excellent active oxygen scavenging ability, tyrosinase inhibitory action, and lactate dehydrogenase inhibitory action.

(Reactive oxygen scavenging action)
The processed product obtained from Terminaria berylica has an excellent active oxygen removing action, exhibits the same activity as SOD existing in the living body, and can quickly erase the active oxygen.

  Active oxygen refers to oxygen and its related compounds activated more than normal oxygen, and in particular in relation to living organisms, superoxide, hydrogen peroxide, hydroxy radical, singlet oxygen and the like can be mentioned. It has been clarified that these active oxygens cause various tissue damages when they are excessively present in the living body.

  In vivo, superoxide, which is usually produced in vivo and serves as a starting material for other active oxygen, is sequentially eliminated by the catalytic action of superoxide dismutase (SOD) contained in cells. However, when the production of superoxide is excessive or when the action of SOD is reduced as in the body of an elderly person, the superoxide is not sufficiently eliminated and the superoxide concentration in the living body becomes high. As a result, tissue damage such as rheumatoid arthritis and Behcet's disease, cataracts, spots, freckles, wrinkles, stiff shoulders, coldness, etc. are caused. Therefore, it is important to quickly eliminate active oxygen in order to prevent various diseases in vivo. In particular, it is possible to prevent or improve spots, freckles, wrinkles, etc. by applying a processed product obtained from Terminaria berylica as an external preparation to the epidermis that is directly exposed to ultraviolet rays and easily generates active oxygen. It can prevent aging.

(Tyrosinase inhibitory action)
When the human epidermis receives ultraviolet rays, as one of the body defense reactions, in order to prevent damage from ultraviolet rays, melanin is generated and the skin is blackened to reduce damage from ultraviolet rays. Normally, this melanin disappears with skin metabolism. However, when skin metabolism deteriorates with aging, melanin accumulates in the deep part of the skin. Furthermore, melanocytes that produce melanin under the influence of ultraviolet rays or the like may produce melanin excessively. Due to these factors, spots, freckles, etc. are formed, resulting in black deposition on the skin.

  By suppressing these series of melanin production processes, the skin becomes beautiful, that is, beautiful skin with few spots and freckles. Melanin is produced by an enzyme called tyrosinase. The processed product obtained from Terminaria berylica inhibits this tyrosinase. Therefore, the effect of inhibiting tyrosinase is expected not only by being applied as an external preparation but also by oral ingestion, and can prevent and improve spots, freckles and the like.

(Lactic acid dehydrogenase inhibitory action)
Collagen, which is a protein that makes up the skin and mucous membranes, is attracting attention not only for maintaining the strength of the skin and gums, but also for making the skin difficult to wrinkle, maintaining the mucosal strength, or improving the mucosal strength. Yes. This collagen synthesis is carried out by dermal fibroblasts. However, the ability to synthesize collagen decreases with age, and wrinkles are formed by increasing the amount of denatured collagen in the dermis. Therefore, the collagen synthesis promoting action is an important action in preventing skin aging and mucosal strength reduction, and collagen synthesis can be promoted by inhibiting lactate dehydrogenase.

  The processed product obtained from Terminaria berylica inhibits this lactate dehydrogenase. Therefore, collagen synthesis can be promoted by using it as an external preparation applied to the skin, the mucous membrane such as the oral cavity, etc., so that the skin can be prevented from wrinkling or the strength of the mucous membrane can be maintained or improved.

  Furthermore, inhibiting lactate dehydrogenase can not only prevent periodontal disease, but also caries, particularly in the oral cavity. One factor of the caries development process is lactic acid produced by lactate dehydrogenase. Since this lactic acid dissolves teeth and becomes caries, caries can be prevented by inhibiting lactate dehydrogenase.

(External preparation)
The external preparation of the present invention contains a processed product obtained from Terminaria berylica as an active ingredient. By administering the external preparation of the present invention to the skin or mucous membrane, prevention or improvement of skin symptoms such as spots, buckwheat, and wrinkles (promoting action on beautiful skin), enhancement of mucosal strength, prevention of diseases such as periodontal disease, Effects such as prevention of dental caries can be obtained.

  In order to acquire such an effect, the quantity of the processed material obtained from Terminaria berylica contained in the external preparation of the present invention is not particularly limited. In the external preparation of the present invention, the processed product (dry mass) is preferably contained in a proportion of 0.00001 mass% to 40 mass%, more preferably 0.001 mass% to 20 mass%.

  The external preparation of this invention can add a various base or additive as needed. Examples of such a base include glycerol, ethanol, paraben, butylene glycol and the like. Examples of additives include excipients such as dextrin, fragrances, pigments, preservatives, silicon-based polymers, acrylic polymers, carboxyvinyl polymers, etc., in order to further improve appearance, feeling of use, storage stability, etc. Thickeners, chelating agents such as EDTA, sweeteners such as sucralose, refreshing agents such as menthol, antiseptic / antifungal agents such as phenoxyethanol, and the like.

  The external preparation of the present invention can be used as pharmaceuticals, quasi drugs, cosmetics, toiletries and the like. For example, it can be processed into a lotion, emulsion, gel, cream, ointment, powder, granule and the like. Specifically, lotion, cosmetic cream, milky lotion, cream, pack, hair tonic, hair cream, shampoo, hair rinse, treatment, body shampoo, facial cleanser, soap, foundation, white powder, lipstick, lip gloss, blusher, eye shadow , Hair styling agents, hair growth agents, aqueous ointments, oily ointments, eye drops, eye wash, ship, gel and the like. Moreover, when applying to an oral cavity, forms, such as a dentifrice, a mouthwash, and a spray, are mentioned.

  Furthermore, various auxiliary agents can be added for the purpose of enhancing or supplementing the action of the external preparation of the present invention. Examples of such auxiliaries include other medicinal ingredients (active oxygen scavengers, antioxidants, anti-inflammatory agents, cell activators, vitamins, hormones, extracts derived from animals and plants having these functions, etc.) , Other oils (unsaturated fatty acids such as linoleic acid, linolenic acid, palmitic acid, DHA, EPA and derivatives thereof, linseed oil, coconut oil, jojoba oil, olive oil, squalane, squalene, horse oil, rice bran oil, castor oil, etc. Oils and their derivatives extracted from plants and animals), moisturizers (collagen or its degradation products, collagen-like peptides contained in carrot extract, etc., soy peptides, amino acids, mucopolysaccharides such as hyaluronic acid, and amino sugars such as chondroitin Sugars such as trehalose, seaweed, water soluble dietary fiber such as alginic acid, glucomannan and pectin ), Surfactants (lecithin and fatty acid esters, such as amino acids derivatives), ultraviolet absorber (zinc oxide, and titanium oxide), and the like ultraviolet absorption enhancer.

  The processed product obtained from Terminaria berylica has an active oxygen scavenging ability, but an external preparation having a more excellent antioxidant action and the like can be obtained by using it together with an active oxygen removing agent and an antioxidant. Such active oxygen scavengers and antioxidants include carotenoids, L-cysteine and their derivatives or salts thereof, flavonoids such as SOD, mannitol, hydroquinone, tryptophan, histidine, quercetin, hesperidin, gallic acid and its Derivatives, button pi extracts, tomato extracts, parsley extracts, melissa extracts, plant extracts such as ougon extracts, BHT, BHA and the like. These active oxygen scavengers and antioxidants may be used alone or in combination of two or more.

Examples of vitamin agents include vitamin A such as retinol, retinal, retinoic acid, 3-dehydroretinol, 3-dehydroretinal, 3-dehydroretinoic acid; α-carotene, β-carotene, γ-carotene, cryptoxanthine, etc. Provitamins A: thiamine (vitamin B ₁ ), riboflavin (vitamin B ₂ ), pyridoxine, pyridoxal, pyridoxamine (above vitamin B ₆ ), cobalamin (vitamin B ₁₂ ), nicotinic acid, nicotinamide, pantothenic acid, biotin ( Vitamin B group such as vitamin H) and folic acid (vitamin M); ascorbic acid (vitamin C); vitamin D such as ergocalciferol (vitamin D ₂ ) and cholecalciferol (vitamin D ₃ ); 7-dehydrocholesterol , Ergosterol Provitamin Ds such as α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol, δ-tocotrienol and other vitamin Es; phylloquinone (vitamin K ₁ ) And vitamin Ks such as menaquinone (vitamin K ₂ ) and menadione (vitamin K ₃ ). Any compound having a stereoisomer or an optical isomer can be used, and a cis, trans mixture and a racemate can also be used.

Next, as vitamin-like agents, essential fatty acids (vitamin F) such as ubiquinone, lipoic acid (thioctic acid), linoleic acid, linolenic acid, arachidonic acid; orotic acid (vitamin B ₁₃ ), carnitine (vitamin B _T ), Myo-inositol, choline, rutin, hesperidin, eliocitrulline and other vitamin Ps; methionine methylsulfonium (vitamin U); pantothenyl alcohol and the like. Any compound having a stereoisomer or an optical isomer can be used, and a cis, trans mixture and a racemate can also be used.

  The vitamins and vitamin-like agent salts include alkali metal salts such as sodium salts and potassium salts; alkaline earth metal salts such as magnesium salts and calcium salts; inorganic salts such as hydrochlorides, sulfates and ammonium salts. Salt; Organic salts such as lactate, acetate, triethanolamine salt and the like can be mentioned. Examples of derivatives such as vitamins include glycosides such as alkyl, alkenyl or aryl esters, phosphate esters, sulfate esters, phosphatidyl esters, glucosides, galactosides, maltosides, and lactosides. These vitamins may be used alone or in combination of two or more.

  In the present invention, the above-mentioned vitamins, vitamin-like agents, and salts and derivatives thereof are derived from natural products obtained by extraction or purification from animals and plants, algae, microorganisms, etc., or chemically or enzymatically reacted. What was synthesize | combined using can be used. Furthermore, a polyphenol compound can also be blended, such as catechol, catechin, epicatechin, gallocatechin, epigallocatechin, gallocatechin gallate, epigallocatechin gallate, tannic acid (galloyl gallic acid), hammelitannin (1,5-diga) Royl Hamamelose), proanthocyanidins, caffeic acid derivatives, gallic acid, various pyrogallol tannins, catechol tannins, tea extracts containing these, grape seed extract, pine bark extract, sweet potato stem extract, etc. .

  Examples of the cell activator include yeast extract, yeast culture supernatant, Asparagus genus plant, avocado (Persea americana Mill.), Aloe genus plant, apricot (Prunus armeniaca L. var. Ansu Maxim). .), Ginkgo biloba L., Fagus japonica Maxim., Carrot (Allium sativum L. f. Pekinense Makino), Panax ginseng CAMeyer, Camellia (Matricaria chamomilla L.), Yellowfin Phellodendron amurense Rupr. And its related plants, cucumber (Cucumis sativus L.), calendula arvensis L., shiitake (Lentinus edodes Sing.), Actinidia chinensis Planch., Equisetum arv .), Horse chestnut (Aesculus hippocastanum L.), garlic (Allium sativum L.), sea bream (Swertia japonica Makino), stephania cephara (Stephania cephara) ntha Hayata, Chicha (Lactuca sativa L.), Capsicum annuum L., Calendula officinalis L., Carrot (Aesculus turbinata Blume), Carrot (Daucus carota L.), Bakryo (Matsuhodo) ) (Poria cocos Wolf), grapes (Vitis vinifera L.), beech (Fagus crenata Blume), loofah (Luffa cylindrica M. Roemen), safflower (Carthamus tinctorius L.), mannenro (Rosmarinus officinalis L.), mandarin orange (Citrus) ) Plants, extracts of plants belonging to the genus, Sapindus mukurossi Gaertn., Purple (Lithospermum officinale L. var. Erythrorhizon Maxim.), Eucalyptus, Lilium, hydroxy fatty acids and their salts And derivatives thereof, nucleic acids and related substances, proteins extracted from eggshell membranes, and mixtures of isomerized sugars. These cell activators may be used alone or in combination of two or more.

Examples of anti-inflammatory agents include cortisone, hydrocortisone, prednisolone, methylprednisolone, dexamethasone, betamethasone, triamcinolone, triamcinolone acetonide, fluocinolone acetonide, fluocinonide, beclomethasone and their phosphates, propionates, acetates, Steroidal anti-inflammatory agents such as succinate; salicylic acid derivatives such as salicylic acid, aspirin, salicylamide, etenzamide and methyl salicylate; indoleacetic acid derivatives such as indomethacin and sulindac; pyrazolidinediones such as phenylbutazone and oxyphenbutazone Derivatives; Anthranilic acid derivatives such as mefenamic acid and flufenamic acid; Propionic acid derivatives such as ibuprofen, ketoprofen and naproxen; Phenylacetic acid derivatives such as Pak, Fenbufen, Bufexamac; Nonsteroidal anti-inflammatory agents such as benzothiazine derivatives such as piroxicam; Glycyrrhizic acid salts and glycyrrhizic acid derivatives such as glycyrrhizic acid, dipotassium glycyrrhizinate, monoammonium glycyrrhizate; Glycyrrhetinate and glycyrrhetinic acid derivatives such as stearyl glycyrrhetinate, glycyrrhetinyl stearate, disodium 3-succinyloxyglycyrrhetinate; azulene derivatives such as guaiazulene, ethyl guaiazulenesulfonate, sodium guaiazulenesulfonate, camazulene; allantoin, aloin, aloe emodin, Shikonin, isobutyl shikonin, acetyl shikonin, isovaleryl shikonin What shikonin derivatives; ginsenoside R _a1, ginsenoside R _a2, Ginsenoside such Ginsenoside R _b1, ginsenoside derivatives such as 20-glucoside Gin Seno glucoside R _f; paeoniflorin, paeonol, Peonoshido, like paeonol derivatives such Peonorido.

  As an anti-inflammatory agent, a plant extract having an anti-inflammatory action can also be used. Examples include Cimicifugae Rhizoma, Zizyphi Fructus, Anemarrhenae Rhizoma, Button Pi (Moutan Cortex), Ryutan (Gentianae Scabrae Radix), Forsythiae Fructus.

  For the purpose of further improving the moisturizing effect and cell activation effect, phospholipids may be used as substances other than those described above. Examples of such phospholipids include phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol (cardiolipin), phosphatidic acid and other glycerophospholipids; lysophosphatidylcholine (lysolecithin), lysophosphatidylethanolamine, lysophosphatidyl Examples include lysoglycerophospholipids such as serine, lysophosphatidylinositol, lysophosphatidylglycerol, and lysophosphatidic acid; sphingophospholipids such as sphingomyelin; and hydrogenated products thereof. As these phospholipids, those obtained by extraction or separation from animals and plants such as soybean and egg yolk, or commercially available cosmetics or raw materials for pharmaceuticals obtained by synthesis by a chemical or enzymatic method can be used.

  Furthermore, examples of the hormone agent include estradiol or a derivative thereof; plant hormone-like substances such as isoflavones such as genestine and daizen.

(Oral skin promoting agent)
The oral beautiful skin promoter of the present invention contains a processed product obtained from Terminaria berylica as an active ingredient. Examples of the form of the oral beauty-promoting agent of the present invention include beverages, granules, tablets, gums, candy, troches, jelly, gummi, tea bags and the like. As described above, in order to obtain a beautiful skin promoting action, it can be taken orally.

  The amount of the processed product obtained from the terminaria berylica contained in the oral skin care promoter of the present invention is not particularly limited, and varies depending on the processed product such as an extract or a dry powder. For example, in the case of an extract of Terminaria berylica, it is contained in the oral skin enhancement agent of the present invention in a proportion of preferably 0.0001% by mass or more, more preferably 0.001% by mass or more in terms of dry mass. , Preferably 90% by mass or less, more preferably 60% by mass or less. In the case of a dry powder of Terminaria berylica, it is preferably contained in the oral skin enhancement agent of the present invention in a proportion of 0.001% by mass or more, more preferably 0.01% by mass or more, preferably 90% by mass or less. More preferably, it is contained in a proportion of 60% by mass or less. When using paste, squeezed juice, and extract powder, the dry mass should be equivalent to the dry powder of Terminaria berylica.

  The intake amount is not particularly limited, and varies depending on the form of the processed product such as an extract or a dry powder. For example, in the case of an extract of Terminaria berylica, the daily intake of an adult is preferably 10 mg or more, more preferably 20 mg or more, preferably 1000 mg or less, more preferably 600 mg or less in terms of dry mass. is there. In the case of dry powder of Terminaria berylica, the daily intake for adults is preferably 100 mg or more, more preferably 200 mg or more, preferably 10,000 mg or less, more preferably 6000 mg or less.

The oral beautiful skin promoter of this invention can contain another component as needed. Examples of such other components include royal jelly, propolis, vitamins (A, B ₁ , B ₂ , B ₆ , B ₁₂ , niacin, C, D, E, K, folic acid, pantothenic acid, biotin, these Derivatives), minerals (iron, magnesium, calcium, zinc, selenium, etc.), chitin, chitosan, lecithin, polyphenols (flavonoids, these derivatives, etc.), carotenoids (lycopene, astaxanthin, zeaxanthin, lutein, etc.), xanthine derivatives ( Caffeine, etc.), protein or peptide (soy protein, collagen, elastin, silk or a degradation product thereof), mucopolysaccharide (hyaluronic acid, chondroitin, dermatan, heparan, heparin, ketalan, etc.), amino sugar (Glucose Min, acetylglucosamine, galactosamine, acetylgalactosamine, neuraminic acid, acetylneuraminic acid, hexosamine, salts thereof, amino acids (proline, glycine, arginine, glutamic acid, etc.), oligosaccharides (isomalto-oligosaccharide, cyclic oligosaccharide, etc.) , Phospholipids (phosphatidylcholine, phosphatidylserine, etc.), sphingolipids and their derivatives (sphingomyelin, ceramide, etc.), sulfur-containing compounds (eg, alliin, sepaene, taurine, glutathione, methylsulfonylmethane), lignans (sesamin, etc.), pearls Examples thereof include powder, carambola extract, pomegranate seed extract, α-lipoic acid or a derivative thereof, CoQ10, and animal and plant extracts containing these, root vegetables (such as ginger), and the like. In particular, vitamins (ascorbic acid, pantothenic acid, biotin, etc.), polyphenols, mucopolysaccharides, amino sugars, carotenoids, proteins or peptides (especially collagen, elastin, laminin and their degradation) that can improve the condition of the skin, mucous membrane, etc. Product), amino acids, phospholipids, sphingolipids, carambola extracts, lychee seed extracts, α-lipoic acid, and CoQ10.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated in detail, this invention is not limited to this range.

(Preparation Example 1)
200 mL of water was added to 5 g of the remaining portion from which the seeds had been removed from the fruit of Terminaria berylica, and the mixture was heated to reflux at 100 ° C for 30 minutes. After extraction, filtration was performed to obtain a filtrate, and 200 mL of water was further added to the residue, followed by heating under reflux at 100 ° C. for 30 minutes. After extraction, the filtrates obtained by filtration were combined and freeze-dried to obtain extract powder A (2.3 g).

(Preparation Example 2)
Extract powder B (2.4 g) was prepared in the same procedure as in Preparation Example 1 except that 50% by volume ethanol aqueous solution was used in place of the water used in Preparation Example 1 and the heating temperature was 80 ° C. Obtained.

(Preparation Example 3)
Extract powder C (0.4 g) was obtained in the same procedure as in Preparation Example 1 except that 2 g of seed was used instead of the remaining part from which the seed was removed from the fruit used in Preparation Example 1.

(Preparation Example 4)
Extract powder D (0.6 g) was obtained in the same procedure as in Preparation Example 2 except that 2 g of seed was used instead of the remaining part from which the seed was removed from the fruit used in Preparation Example 2.

(Example 1: Evaluation of active oxygen scavenging ability)
Samples 1 to 4 were prepared by dissolving the extract powders A to D obtained in Preparation Examples 1 to 4 in water so as to be 3 mg / mL, respectively. Subsequently, using a measurement kit (SOD Test Wako, Wako Pure Chemical Industries, Ltd.), the SOD activity of Samples 1 to 4 was measured, and the SOD-like activity (unit) per 1 mg of the extract powder was calculated. For comparison, SOD-like activity was calculated using a green tea extract (manufactured by Mitsui Norin Co., Ltd.). The results are shown in Table 1. The measurement result is an average value of triple measurements.

  As shown in Table 1, it is known that all processed products (extract powder) obtained from the fruits of Terminaria berylica have SOD-like activity, particularly in parts other than seeds. It can be seen that it has a much better SOD-like activity than the green tea extract. That is, it can be seen that the processed product obtained from the fruit of Terminaria berylica has an excellent active oxygen scavenging ability and can be used as an external preparation or an oral skin beautifying agent.

(Example 2: Evaluation of tyrosinase inhibitory action)
Tyrosinase inhibitory activity was measured as follows.

  First, the extract powders A to D obtained in Preparation Examples 1 to 4 were dissolved in purified water so as to have a concentration of 0.3% by mass to prepare test solutions 1 to 4, respectively.

  A comparative solution was prepared by dissolving ascorbic acid, which is known to have tyrosinase inhibitory activity, in purified water to a concentration of 0.3% by mass.

  Next, 90 μL of 1/15 M phosphate buffer (pH 6.8) and 10 μL of 1.1 unit / μL tyrosinase-containing solution (manufactured by Wako Pure Chemical Industries, Ltd.) are added to 100 μL of test solutions 1 to 4 and the comparative solution. And kept at 37 ° C. for 10 minutes. Next, 100 μL of 0.03% DOPA (manufactured by Wako Pure Chemical Industries, Ltd.) was added to each solution and reacted at 37 ° C. for 5 minutes. After the reaction, the absorbance at 480 nm of each solution was measured (measured value is A). In the same manner as in the tyrosinase solution instead of the 1 / 15M phosphate buffer, the absorbance of the blank was measured (measured value is a).

  As controls, purified water was used instead of each test solution or comparative solution, and the absorbance of the reaction solution (measured value is B) and flank absorbance (measured value is b) in the same manner as described above. Was measured. After measuring each value, the inhibitory activity (%) of tyrosinase was measured using the following formula (I).

  The results are shown in Table 2. The values in the table are the mean and standard deviation of triplicate measurements.

  As shown in Table 2, it can be seen that the processed product of Terminaria berylica, in particular, the processed product (extract powder) obtained from the portion excluding the fruit seeds, has an inhibitory activity equivalent to that of ascorbic acid. Therefore, it can be seen that the external preparation or the skin-beautifying agent for oral use of the present invention has an excellent tyrosinase inhibitory activity, and can prevent or improve spots, freckles and the like.

(Example 3: Evaluation of lactate dehydrogenase inhibitory action)
Lactate dehydrogenase inhibitory activity (%) was measured using a lactate dehydrogenase measurement kit (LDH Cytotoxicity Detection Kit, manufactured by Takara Bio Inc.) using 50 μL of the above test solutions 1 to 4. The results are shown in Table 3. The values in the table are the mean and standard deviation of triplicate measurements.

  As shown in Table 3, it can be seen that the processed product of Terminaria berylica, in particular, the processed product (extract powder) obtained from the portion excluding the fruit seeds, has excellent lactate dehydrogenase activity. Therefore, the external preparation or oral skin-beautifying agent of the present invention can exhibit the effects of preventing wrinkles, maintaining or improving mucosal strength, preventing periodontal disease, and preventing caries by inhibiting the production of lactic acid or promoting collagen synthesis. I understand.

(Production Example 1)
Using the following raw materials, a beverage form oral skin beautification promoter (500 mL) was prepared.

<Blending ingredients> Blending amount (mass%)
Extract powder A 0.003 obtained in Preparation Example 1 above
Hyaluronic acid (Seikagaku Corporation) 0.001
Collagen peptide (Nippi Co., Ltd.) 0.001
Elastin peptide (manufactured by Aswell Corporation) 0.001
Chondroitin complex protein (manufactured by Maruha Corporation) 0.001
Sodium ascorbate (BASF Takeda Vitamin Co., Ltd.) 0.1
Cystine (Ajinomoto Co., Inc.) 0.001

(Production Example 2)
A lotion was prepared using the following raw materials.

<Blending ingredients> Blending amount (g)
Extract powder A 0.1 obtained in Preparation Example 1 above
Glycerin 6
Propylene glycol 4
Oleyl alcohol 0.1
Polyoxyethylene lauryl ether 1
Ethanol 5
Phenoxyethanol 0.1
Ascorbic acid 1
Purified water 82.7

(Production Example 3)
A W / O type emollient cream was prepared using the following raw materials.

<Blending ingredients> Blending amount (mass%)
Extract powder B 0.1 obtained in Preparation Example 2 above
Microcrystalline wax 3
Lanolin 3
Vaseline 5
Squalane 9
Olive oil 12
Sorbitan sesquioleate 3
Polyoxyethylene sorbitan trioleate (20E.O.) 1
Sorbitol 9
Purified water 53.9
Preservative appropriate amount Fragrance proper amount

  ADVANTAGE OF THE INVENTION According to this invention, the external preparation which has the outstanding active oxygen scavenging ability, the tyrosinase inhibitory effect, and the lactate dehydrogenase inhibitory effect which contain the processed material obtained from terminaria berylica as an active ingredient is provided. This external preparation is useful for preventing or improving stains, buckwheat, wrinkles, etc., maintaining or improving mucosal strength, preventing periodontal disease, and preventing caries based on these effects. Furthermore, according to the present invention, an oral skin beautification promoter is provided, and the same effects as the external preparation of the present invention can be exhibited.

Claims (2)

  An external preparation containing a processed product obtained from Terminaria berylica as an active ingredient.   An oral skin enhancement agent containing a processed product obtained from Terminaria berylica as an active ingredient.