JP2012201670A - Preparation for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a preparation for external use which further improves moisture retention of skin compared with a preparation for external use which contains only collagen as an ingredient for improvement of moisture retention of skin.SOLUTION: The preparation for external use includes a pine bark extract, collagen, and growth factors (especially EGF or EGF-like component) so that it can improve moisture retention of skin compared to a preparation for external use which contains only collagen as an ingredient for improvement of moisture retention of skin.

Description

  The present invention relates to an external preparation.

  Conventionally, various external preparations have been developed that are effective in preventing wrinkles, improving skin firmness and gloss, and improving moisture retention. In these external preparations, various components are blended in order to improve the moisture retention of the skin. In recent years, external preparations (for example, cosmetics) containing collagen have been known for the purpose of improving the moisture retention of the skin.

JP 2002-20263 A

  However, in the diversification of external preparations, it has been desired to provide an external preparation that can further improve the moisture retention of the skin as compared with an external preparation containing collagen.

  The present invention is in response to the above-mentioned demand, and an external preparation capable of further improving the skin moisturizing property as compared with an external preparation containing only collagen as a component for improving the skin moisturizing property. The purpose is to provide.

  In order to achieve the above-mentioned object, the external preparation according to claim 1 contains a pine bark extract, collagen and a growth factor. In the present invention, the growth factor is also referred to as a growth factor, and EGF (Epidmal Growth Factor: epidermal growth factor / epidermal growth factor), FGF (Fibroblast Growth Factor), TGF (Transforming Growth Factor): trans Forming growth factor), IGF (Insulin-like Growth Factor), HGF (Hepatocyte Growth Factor), EGF-like component (EGF-like component) has the same action as EGF in the body. Component). In the present invention, the term “collagen” is not limited to a collagen protein, but includes a collagen peptide obtained by hydrolyzing a collagen protein and an atelocollagen obtained by treating a collagen molecule with a protease and removing a telopeptide portion. is there.

  The external preparation according to claim 2 is the external preparation according to claim 1, wherein the growth factor is an EGF (Epidmal Growth Factor) component.

  The external preparation according to claim 3 is the external preparation according to claim 1, wherein the growth factor is an EGF (Epidmal Growth Factor) -like component contained in a swallow's nest. The EGF-like component refers to a component that exhibits the same action as EGF in the body.

  The external preparation according to claim 4 is the external preparation according to claims 1 to 3, wherein the growth factor is blended in an amount of 0.0001 to 0.5% by weight based on the total weight of the external preparation.

  The external preparation according to claim 5 is the external preparation according to any one of claims 1 to 4, wherein 0.01 to 100 parts by weight of the pine bark extract is added to 1 part by weight of the growth factor. Yes.

  The external preparation according to claim 6 is the external preparation according to any one of claims 1 to 5, wherein the pine bark extract is blended in an amount of 0.0001 to 1% by weight based on the total weight of the external preparation. .

  Since the external preparation of the present invention contains a pine bark extract, collagen and a growth factor (especially EGF or EGF-like component), it is compared with an external preparation containing only collagen as a component for improving the moisture retention of the skin. Thus, there is an effect that the moisture retention of the skin can be further improved.

  Hereinafter, embodiments of the present invention will be described. The present invention is not construed as being limited by the description of the embodiments described later, and various modifications can be made within the scope of the claims.

  The external preparation of the present invention is characterized by blending a pine bark extract, collagen and a growth factor. The growth factor to be blended in the external preparation of the present invention is characterized by being an EGF (Epidmal Growth Factor) component or an EGF-like component contained in a swallow's nest. The external preparation of the present invention is characterized in that the growth factor is blended in an amount of 0.0001 to 0.5% by weight based on the total weight of the external preparation. Moreover, the external preparation of this invention is characterized by mix | blending 0.01-100 weight part of pine bark extracts with respect to 1 weight part of growth factors. Furthermore, the external preparation of the present invention is characterized in that the pine bark extract is blended in an amount of 0.0001 to 1% by weight based on the total weight of the external preparation.

(About pine bark extract)
The pine bark extract is extracted from the bark of French coastal pine (Pinus Martina), larch, black pine, red pine, Japanese black pine, pine pine, Korean pine, high pine, rhyukyu pine, Utsukushima, Japanese pine, white pine, Canada The product is preferably used. Among these, a bark extract of French coastal pine is preferably used.

  French coastal pine is a marine pine that grows on the Atlantic coast of southern France. The bark of French coastal pine contains organic acids and other physiologically active components in addition to proanthocyanidins, which are flavonoids, as main components. This main component, proanthocyanidins, is known to have a strong antioxidant action to remove active oxygen.

  The pine bark extract is obtained by extracting the above-mentioned pine bark with water and / or an organic solvent. When water is used, warm water or hot water is used. As an organic solvent used for extraction, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, ethyl methyl ketone, Organic solvents acceptable for the production of foods or drugs such as glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, edible oils and fats, 1,1,1,2-tetrafluoroethane, 1,1,2-trichloroethene Preferably used. These water and organic solvents may be used alone or in combination. In particular, hot water, hydrous ethanol, hydrous propylene glycol and the like are preferably used.

  The method for extracting the pine bark extract from the pine bark is not particularly limited, and for example, a warm extraction method, a supercritical fluid extraction method, or the like is used.

  The supercritical fluid extraction method is a method of performing extraction using a supercritical fluid that is a fluid that exceeds the critical point (critical temperature, critical pressure) of a gas-liquid substance. As the supercritical fluid, carbon dioxide, ethylene, propane, nitrous oxide (laughing gas) or the like is used, and carbon dioxide is preferably used.

  In the supercritical fluid extraction method, an extraction process for extracting a target component with a supercritical fluid and a separation process for separating the target component and the supercritical fluid are performed. In the separation step, any one of extraction separation by pressure change, extraction separation by temperature change, and extraction separation using an adsorbent / absorbent may be performed.

  Moreover, you may perform supercritical fluid extraction by the entrainer addition method. In this method, for example, ethanol, propanol, n-hexane, acetone, toluene, other aliphatic lower alcohols, aliphatic hydrocarbons, aromatic hydrocarbons, and ketones are added to the extraction fluid in an amount of 2 to 20 W / V. %, And by performing supercritical fluid extraction using this fluid, the solubility of the target extract such as OPC (oligomeric proanthocyanidin) and catechins in the extraction solvent is dramatically increased. Or a method for enhancing the selectivity of separation, and obtaining an efficient pine bark extract.

  Since the supercritical fluid extraction method can be operated at a relatively low temperature, it can be applied to substances that are altered or decomposed at high temperatures, the advantage that the extraction fluid does not remain, and the recycling of the solvent is possible. There is an advantage that the process can be omitted and the process becomes simple.

  Extraction from pine bark may be performed by a liquid carbon dioxide batch method, a liquid carbon dioxide reflux method, a supercritical carbon dioxide reflux method, or the like, in addition to the extraction method described above.

  For extraction from pine bark, a plurality of extraction methods may be combined. By combining a plurality of extraction methods, it becomes possible to obtain pine bark extracts having various compositions.

  It is safe to purify the pine bark extract obtained by the above-described extraction by ultrafiltration or column method or batch method using an adsorptive carrier (Diaion HP-20, Sephadex-LH20, chitin, etc.). From the viewpoint of sex.

  Specifically, the pine bark extract to be blended with the external preparation of the present invention is prepared by the following method, but this is illustrative and is not limited to this method.

  1 kg of French coastal pine bark is placed in 3 L of a saturated aqueous solution of sodium chloride and extracted at 100 ° C. for 30 minutes to obtain an extract (extraction process). Thereafter, the extract is filtered, and the resulting insoluble matter is washed with 500 mL of a saturated solution of sodium chloride to obtain a washing solution (washing step). The extract and washing solution are combined to obtain a crude extract of pine bark.

  Next, 250 mL of ethyl acetate is added to the crude extract and the phases are separated, and the process of recovering the ethyl acetate layer is repeated 5 times. The collected ethyl acetate solutions are combined and added directly to 200 g of anhydrous sodium sulfate for dehydration. The ethyl acetate solution is then filtered and the filtrate is concentrated in vacuo until the original volume is reduced to one fifth. Then, the concentrated ethyl acetate solution is poured into 2 L of chloroform, and the precipitate obtained by stirring is collected by filtration. Thereafter, the precipitate is dissolved in 100 mL of ethyl acetate, and then added again to 1 L of chloroform and precipitated for washing twice. By this method, for example, about 5 g of pine bark extract containing 20% by weight or more of proanthocyanidins having a degree of polymerization of 2 to 4 and containing 5% by weight or more of catechins is obtained. Here, the content of the specific component in the extract is a value based on the dry mass of the extract, and the same applies to the following.

  Pine bark extract contains proanthocyanidins as one of the main active ingredients. Proanthocyanidins refer to a group of compounds consisting of a condensation polymer having a degree of polymerization of 2 or more, with flavan-3-ol and / or flavan-3,4-diol as a structural unit. Proanthocyanidins are powerful antioxidants produced by plants and are intensively contained in plant leaves, bark, fruit peels and seeds. This proanthocyanidin is a substance that cannot be produced in the human body.

  When a pine bark extract containing proanthocyanidins is ingested, an excellent effect of improving lipid metabolism is obtained. The pine bark extract contains a condensed polymer having a polymerization degree of 2 or more as proanthocyanidins, and further contains catechin and the like. In particular, proanthocyanidins containing a large amount of a condensation polymer having a low degree of polymerization are preferably used. The condensation polymer having a low polymerization degree is preferably a condensation polymer having a polymerization degree of 2 to 30 (2 to 30 mer), more preferably a condensation polymer having a polymerization degree of 2 to 10 (2 to 10 mer). Further, a condensation polymer (2 to 4 mer) having a polymerization degree of 2 to 4 is more preferable. Proanthocyanidins that are condensation polymers having a degree of polymerization of 2 to 4 (2 to 4 mer) are particularly easily absorbed into the body. In the present specification, the polymer having a degree of polymerization of 2 to 4 is referred to as oligomeric proanthocyanidin (hereinafter referred to as “OPC”).

  The pine bark extract contains 2-4 mer of proanthocyanidins (ie, OPC), based on the dry mass of the pine bark extract, 15% by weight or more, more preferably 20% by weight or more, and further preferably 30% by weight. More preferably, it is an extract containing the above.

  Further, the pine bark extract is preferably an extract containing pentamer or more proanthocyanidins in an amount of 10% by weight or more, more preferably 15% by weight or more based on the dry mass of the pine bark extract.

  The pine bark extract may further contain catechins. The content of catechins in the pine bark extract is preferably 5% by weight or more, more preferably 10% by weight or more, based on the dry mass of the pine bark extract.

  Catechins can be extracted together with proanthocyanidins (for example, OPC) by the extraction method described above. Catechin is a general term for polyhydroxyflavan-3-ol. As catechins, (+)-catechin (referred to as catechin in a narrow sense), (−)-epicatechin, (+)-gallocatechin, (−)-epigallocatechin, epigallocatechin gallate, epicatechin gallate, afzelechin, etc. It has been known.

  In addition to the above (+)-catechin, gallocatechin, afzelekin, and (+)-catechin or 3-galloyl derivatives of gallocatechin have been isolated from the pine bark extract. Catechins are known to have a carcinogenic inhibitory effect, an active oxygen and free radical scavenging effect, and an antioxidant effect. In addition, catechins are known to have an antidiabetic effect that suppresses an increase in blood sugar. Catechins are poor in water solubility by themselves and have low physiological activity, but in the presence of OPC, catechins have the property of being activated at the same time as water solubility increases. Therefore, catechins work effectively when present together with OPC.

  The catechins are preferably contained in the pine bark extract at 5% by weight or more. More preferably, the pine bark extract containing 20% by weight or more of OPC and 10% by weight or more of proanthocyanidins having a pentamer or more contains 5% by weight or more of catechins. For example, when the catechin content of the pine bark extract is less than 5% by weight, the catechins may be added so that the content is 5% by weight or more. That is, it is more preferable to use a pine bark extract containing 5% by weight or more of catechins, 20% by weight or more of OPC, and 10% by weight or more of proanthocyanidins of pentamer or more.

(About collagen)
Collagen blended in the preparation for external use of the present invention is a general term for fibrous hard proteins constituting the living body of animals, soluble protein (collagen peptide) obtained by hydrolyzing this, and collagen molecules treated with protease. The concept includes atelocollagen from which the telopeptide moiety has been removed, and the base animal may be a land animal or aquatic animal. As the specific source animal, terrestrial animals such as cattle, pigs and sheep, and aquatic animals such as fish are preferable. Preferred examples include those obtained by extracting the skeletal proteins of these animals with an aqueous carrier or hydrolyzing with a protease or the like and then extracting the soluble portion. Particularly preferred are those based on fish, hydrolyzed and solubilized. Moreover, as for a preferable molecular weight, an average molecular weight is 3000-100,000. In order to obtain such an average molecular weight collagen, it is preferable to reduce the molecular size by hydrolyzing the collagen, and to adjust the molecular weight distribution by ultrafiltration if desired. In addition, since there exists a commercial item which can be utilized as an external preparation (for example, cosmetics) already, you may use such collagen.

(About growth factors)
The growth factor blended in the external preparation of the present invention is a general term for endogenous proteins that promote proliferation and differentiation of specific cells in the body. As a growth factor mix | blended with the external preparation of this invention, as mentioned above, EGF, FGF, TGF, IGF, HGF, an EGF-like component can be illustrated.

  In addition, as an EGF-like component mentioned above, the EGF-like component contained in a swallow's nest can be illustrated. For this EGF-like component, there is already a swallow's nest that can be used as an external preparation. Therefore, after extracting the EGF-like component from this swallow's nest in advance, it may be added to the external preparation. In addition to extracting only the EGF-like component, an extract may be extracted from the swallow's nest and the swallow's nest extract may be blended in the external preparation.

(About the external preparation of this invention)
The external preparation of the present invention contains a pine bark extract, collagen and a growth factor.

  Moreover, the amount of the growth factor to be blended in the external preparation of the present invention is not particularly limited, but is preferably 0.0001 to 0.5% by weight based on the total weight of the external preparation.

  The amount of the pine bark extract to be blended in the external preparation of the present invention is not particularly limited, but it is preferably 0.01 to 100 parts by weight with respect to 1 part by weight of the growth factor. It is preferable to add 0.001-1% by weight based on the total weight of the agent.

  In addition to the pine bark extract, collagen, and growth factors, the external preparation of the present invention includes groups usually used by those skilled in the art for external preparations as necessary in order to further improve the appearance, feeling of use and storage stability. Agents and additives may be included. Furthermore, various auxiliary agents can be added for the purpose of enhancing or supplementing the function of the external preparation of the present invention.

  Examples of the base include glycerol, ethanol, paraben, butylene glycol, and the like. The above-mentioned additives include excipients (silicon-based polymers), fragrances, pigments, preservatives (parabens, etc.), thickeners (silicon-based polymers, acrylic polymers, carboxyvinyl polymers, etc.), chelating agents. (Such as EDTA), sweeteners (such as sucralose), refreshing agents (such as menthol), antiseptic / antifungal agents (such as phenoxyethanol), and the like.

  Examples of the auxiliary agent include other medicinal components and other oils (unsaturated fatty acids such as linoleic acid, linolenic acid, palmitic acid, DHA, EPA, and derivatives thereof, linseed oil, coconut oil, jojoba oil, Olive oil, squalane, squalene, horse oil, rice bran oil, castor oil and other oils and derivatives thereof, moisturizers (collagen or its degradation products, collagen-like peptides such as carrot extract, soy peptide, Amino acids, mucopolysaccharides such as hyaluronic acid, amino acids such as chondroitin, saccharides such as trehalose, seaweeds, water-soluble dietary fibers such as alginic acid, glucomannan, pectin, phospholipids, etc.), surfactants (lecithin and fatty acid esters, Amino acid derivatives), UV absorbers (such as zinc oxide and titanium oxide) Absorption enhancers, and the like.

  Medicinal components that are one of the above-mentioned adjuvants include polyphenol compounds, vitamins (vitamins, vitamin-like agents, salts or derivatives thereof), anti-inflammatory agents, cell activators, hormones, etc. The extract derived from animals and plants which has an effect is mentioned.

  Examples of the polyphenol compound include catechol, gallocatechin, epigallocatechin, gallocatechin gallate, epigallocatechin gallate, tannic acid (galloyl gallic acid), hamamelitannin (1,5-digalloyl hamamelose), caffeic acid derivative , And gallic acid. The polyphenol compound includes various pyrogallol tannins, catechol tannins and the like.

  As vitamin preparations, vitamin A (retinol, retinal, retinoic acid, 3-dehydroretinol, 3-dehydroretinal, 3-dehydroretinoic acid, etc.); provitamin A (α-carotene, β-carotene, γ-carotene) Vitamin B group (thiamin (vitamin B1), riboflavin (vitamin B2), pyridoxine, pyridoxal, pyridoxamine (above, vitamin B6), cobalamin (vitamin B12), nicotinic acid, nicotinamide, pantothenic acid, biotin (Vitamin H), folic acid (Vitamin M), etc .; Ascorbic acid (Vitamin C); Vitamin D (Ergocalciferol (Vitamin D2), Cholecalciphenol (Vitamin D3), etc.), Provitamin D (7-dehydro) Cholestero Vitamin E (α-tocophenol, β-tocophenol, γ-tocophenol, δ-tocophenol, α-tocotrienol, β-tocotrienol, γ-tocotrienol, δ-tocotrienol, etc.); vitamin K (Phylloquinone (vitamin K1), menaquinone (vitamin K2), menadione (vitamin K3), etc.) and the like. Any one having a stereoisomer or an optical isomer can be used, and a cis- and trans-isomer mixture or a ceramic body can also be used.

  Vitamin-like factors include essential fatty acids (vitamin Fs) such as linoleic acid, linolenic acid, and arachidonic acid; orotic acid (vitamin B13), carnitine (vitamin BT), myo-inositol, choline, rutin, hesperidin, and eriocitrulline Vitamin Ps such as methionine methylsulfonium (vitamin U); pantothenyl alcohol and the like. Any one having a stereoisomer or an optical isomer can be used, and a cis- and trans-isomer mixture or a ceramic body can also be used.

  Examples of the salts of vitamins or vitamin-like agents described above include alkali metal salts such as sodium salts and potassium salts; alkaline earth metal salts such as magnesium salts and calcium salts; inorganic salts such as hydrochlorides, sulfates and ammonium salts Organic salts such as lactate, acetate and triethanolamine salt; Derivatives of the above-mentioned vitamins or vitamin-like agents include glycosides such as alkyl esters, alkenyl esters, aryl esters, phosphate esters, sulfate esters, phosphatidyl esters, glucosides, galactosides, maltosides, and lactosides.

  In the present invention, the above-mentioned vitamins, vitamin-like agents, or salts or derivatives thereof are naturally derived from animals and plants, algae, microorganisms, etc. and purified, or chemical or enzymatic reactions are used. Can be used.

  Cell activators include yeast extract and yeast culture supernatant, Asparagus genus plant, Avocado (Pcrska amicricana Mill.), Aloe genus plant, Apricot (Prunus armenica L. var. Ansu Maxim.). Ginkgo biloba L., Fagus japonica Maxim., Allium sativum L. pekinense Makino, Panax ginseng C. A. Ginseng (Panax ginseng C.). (Phellodendron amurense Rupr.) And its related plants, cucumber (Cu umis sativus L.), calendula arvensis L., shiitake mushroom (Lentinus edodes Sing.), actinidia chinensis Planch., sugiena um. Garlic (Allium sativum L.), Sembria (Swartia japonica Makino), Tamazaki cephalantha Hayata, Tachi (Lactuca sativa um), L endula officinalis L., Japanese cypress (Aesculus turbinata Blume), carrot (Daucus carota l L), papaya (Pana cocoa Wolf), grape (Vitis vinifera L.), grape (Vitis vinifera L.) M. Roemen, safflower (Carthamus tinctorius L.), mannenro (Rosmarinus officinalis L.), Citrus genus plant, Sapindus mukurossi Gaertn. ), Murasaki (Lithosperum officinalale L. var. Erythrorhizon Maxim.), Eucalyptus plants, Lilyum plant extracts, hydroxy fatty acids and their derivatives, their salts, nucleic acids and related substances, eggshells Examples thereof include a mixture of protein and isomerized sugar extracted from a membrane. The cell activator may be used alone or in combination of two or more.

  Phospholipids can also be used as cell activators. Phospholipids are useful because they also have a moisturizing effect. Phospholipids include phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol (cardiolipin), glycerophospholipids such as phosphatidic acid, lysophosphatidylcholine (lysolecithin), lysophosphatidylethanolamine, lysophosphatidylserine, Examples thereof include lysoglycerophospholipids such as phosphatidylinositol, lysophosphatidylglycerol, and lysophosphatidic acid, sphingophospholipids such as sphingomyelin, and hydrogenated products thereof. Phospholipids can be obtained, for example, by extraction and separation from animals and plants such as soybeans and egg yolk, or synthesis by chemical or enzymatic methods.

  Anti-inflammatory agents include cortisone, hydrocortisone, prednisolone, methylprednisolone, dexamethasone, betamethasone, triamcinolone, triamcinolone acetonide, fluocinolone acetonide, fluocinonide, beclomethasone, salts thereof (phosphate, propionate, acetate, Steroidal anti-inflammatory agents such as succinate, etc .; salicylic acid, salicylic acid derivatives (such as aspirin, salicylamide, etenzaamide, methyl salicylate), indoleacetic acid derivatives (such as indomethacin, sulindac), pyrazolidinedione derivatives (phenylbutazone, Oxyphenbutazone), anthranilic acid derivatives (mefenamic acid, flufenamic acid, etc.), propionic acid derivatives (ibuprofen, ketoprofen, naproxen, etc.), Non-steroidal anti-inflammatory agents such as nylacetic acid derivatives (such as diclofenac, fenbufen, and bufexamac), benzothiazine derivatives (such as piroxicam); glycyrrhizic acid or salts or derivatives thereof (such as dipotassium glycyrrhizinate, monoammonium glycyrrhizinate); glycyrrhetinic acid or its Salts or derivatives (stearyl glycyrrhetinate, glycyrrhetinyl stearate, disodium 3-succinyloxyglycyrrhetinate, etc.); azulene derivatives (eg, guaiazulene, ethyl guaiazulene sulfonate, sodium guaiazulene sulfonate, camazulene); allantoin; aloin; aloe emodin; shikonin or Derivatives thereof (isobutyl shikonin, acetyl shikonin, isovaleryl shikonin, etc.); Senoshido (Ginsenoside Ra1, Ginsenoside Ra2, etc. ginsenoside Rb1); ginsenosides derivatives (20-glucoside Gin Seno glucoside Rf etc.); paeoniflorin; paeonol and its derivatives (Peonoshido, Peonorido) and the like.

  Moreover, a plant extract can also be utilized as an anti-inflammatory agent. Examples of the available ones include Ogon (Scutellariae Radix), Licorice (Glycyrrhizae Radix), Kujin (Sophorae Radix), Psycho (Bupurei Radix), Zaku (Paeoniae Radix), Icium (Paeoniae Radiox), And extracts such as Timo (Anemarrhenae Rhizoma), Buttonpi (Moutan Cortex), Ryutan (Gentianae Scabrae Radix), Forsythiae Fructus and the like.

  Examples of the hormone agent include estradiol or a derivative thereof, and a plant hormone-like substance (for example, isoflavones such as genestin and daizen).

  When applying the external preparation of the present invention to the mucous membrane of the oral cavity, the components listed below may be contained as necessary. Examples of such components include royal jelly, propolis, vitamins (A, B1, B2, B6, B12, niacin, C, D, E, K, folic acid, pantothenic acid, biotin, derivatives thereof, etc.), minerals (Iron, magnesium, calcium, zinc, etc.), selenium, chitin, chitosan, lecithin, polyphenols (flavonoids, derivatives thereof, etc.), carotenoids (lycopene, astaxanthin, zeaxanthin, lutein, etc.), xanthine derivatives (caffeine, etc.), proteins Or peptides (soy protein, collagen, elastin, silk, degradation products thereof), mucopolysaccharides (hyaluronic acid, chondroitin, dermatan, heparan, heparin, ketalan, derivatives thereof, etc.), amino sugars (glucosamine, acetyl group) Cosamine, galactosamine, acetylgalactosamine, neuraminic acid, acetylneuraminic acid, hexosamine, salts thereof, amino acids (proline, glycine, arginine, glutamic acid, etc.), oligosaccharides (isomalto-oligosaccharides, cyclic oligosaccharides, etc.), phospholipids (Phosphatidylcholine, phosphatidylserine, etc.), sphingolipids and their derivatives (sphingomyelin, ceramide, etc.), sulfur-containing compounds (eg, alliin, sepaene, taurine, glutathione, methylsulfonylmethane), lignans (sesamin, etc.), pearl powder, gorenshi Examples thereof include extracts, pomegranate seed extracts, animal and plant extracts containing these, root vegetables (such as ginger), and the like. In particular, mucopolysaccharides, amino sugars, carotenoids, proteins or peptides (particularly collagen, elastin, laminin and their degradation products), amino acids, phospholipids and sphingolipids can be preferably contained.

  The external preparation of the present invention can take any form applicable to the skin or mucosa (including oral mucosa) for transdermal administration and transmucosal administration. The dosage form of the external preparation of the present invention can be, for example, a lotion, emulsion, gel, cream, ointment, powder, granule, tablet and the like. The external preparation of the present invention can be used as cosmetics, pharmaceuticals, quasi drugs, toiletries, oral preparations and the like. These contain components usually used by those skilled in the art, if necessary, and can be prepared according to methods usually used by those skilled in the art.

  In the case of cosmetics, for example, it can also be used as a cleaning cosmetic, a basic cosmetic, a finishing cosmetic, and a hair cosmetic. After mixing polar water and an active ingredient beforehand, the cosmetics which improved the effect of the active ingredient can be obtained by adding the ingredient normally used by those skilled in the art. Examples of cleansing cosmetics include face wash, soap, shampoo, hair rinse, treatment, body shampoo and the like. Examples of basic cosmetics include lotions, cosmetic creams, milky lotions, packs and the like. Examples of the finishing cosmetic include foundation, white powder, lipstick, lip gloss, blusher, and eye shadow. Examples of the pharmaceutical include hair restorer, aqueous ointment, oily ointment, eye drops, eye wash, ship, gel and the like. Examples of oral preparations to be applied to the oral cavity include dentifrices, mouthwashes, sprays, and troches.

  The external preparation of the present invention can be applied to skin or mucosa (including oral mucosa) for transdermal administration and transmucosal administration. The external preparation of the present invention is in the form as described above, and can be administered or applied to an individual according to a procedure usually used for administration or application in that form (for example, application, dissolution in the oral cavity, etc.).

  Examples of the present invention will be described below. Note that the present invention is not construed as being limited to the examples, and various modifications can be made within the scope of the claims.

(Skin moisture measurement test using external preparation)
Test for measuring the moisture content of the skin with a comparative example (skin moisture content measurement test) for an external preparation containing pine bark extract, collagen and EGF (hereinafter referred to as “test external preparation” in this example) And the superiority of this test external preparation was evaluated.

  Before carrying out the skin moisture content measurement test, first, five types of solutions including test external preparations and Comparative Examples 1 to 4 shown in Table 1 were prepared. Specifically, pine bark extract powder (trade name: Flavangenol, manufactured by Toyo Shinyaku Co., Ltd. ("Flavengenol" is a registered trademark of Toyo Shinyaku Co., Ltd.)), hydrolyzed collagen powder , EGF powder and pure water were mixed. At this time, 0.01% by weight of Flavangenol (registered trademark), 0.1% by weight of collagen, 0.0001% by weight of EGF, 0.0001% by weight of EGF, and the remainder A solution in pure water was prepared.

  As Comparative Example 1, Flavangenol (registered trademark) powder, hydrolyzed collagen powder and pure water were mixed. At this time, 0.01% by weight of Flavangenol (registered trademark) was blended with 0.1% by weight of collagen with respect to the entire mixed solution, and a solution was prepared with the remainder being pure water. Further, as Comparative Example 2, Flavangenol (registered trademark) powder, EGF powder and pure water were mixed. At this time, 0.01% by weight of Flavangenol (registered trademark) was blended with 0.0001% by weight of EGF with respect to the whole mixed solution, and a solution was prepared with the balance being pure water. Furthermore, as Comparative Example 3, hydrolyzed collagen powder, EGF powder and pure water were mixed. At this time, 0.1% by weight of collagen and 0.0001% by weight of EGF were blended with respect to the entire mixed solution, and a solution was prepared with the balance being pure water. As Comparative Example 4, only pure water was used.

(Table 1)

  The skin moisture content measurement test was carried out in the following order. First, the forearm portions of both arms of two subjects were washed with soap and towel-dried. Thereafter, the subject was rested and acclimated for 15 minutes indoors. During acclimatization, a total of five 4 cm × 4 cm frames were written in black magic on both forearms. After 15 minutes of acclimatization, the amount of water in the frame was measured using a measuring device.

  Next, 60 μL of a total of 5 solutions of the test external preparation and Comparative Examples 1 to 4 were dropped into separate frames, and spread with fingers so that each solution spread uniformly within the frame. And after making a test subject into a resting state indoors, after 5 minutes of application | coating and 30 minutes after application | coating, the moisture content in each frame was measured using the measuring apparatus. The moisture content in the frame was measured using a skin horn layer moisture content measuring device: SKICON-200EX manufactured by IBS Co., Ltd. The indoor environment was set to a temperature of 26 to 28 ° C. and a humidity of 40 to 50%.

  After measuring the moisture content in each frame, the degree of change in moisture content after 5 minutes of application and the degree of change in moisture content after 30 minutes of application were calculated using the following formula 1.

(Formula 1)
Change in water content = measured value of water content after solution application / measured value of water content before solution application

  As can be seen from Equation 1, if the degree of change in the amount of water is less than 1, it indicates that the amount of water after application is less than the amount of water before application. The amount indicates that it is equal to or greater than the amount of water before coating. In particular, the higher the amount of water after application than the amount of water before application, that is, the higher the moisture retention of the solution, the greater the degree of change in the amount of water than 1 in Equation 1.

  Table 2 shows the degree of change in water content (average value of each subject) after 5 minutes of solution application and 30 minutes of solution application.

(Table 2)

  As shown in Table 2, for the degree of change (average value) in the amount of water 5 minutes after solution application, the values of Comparative Examples 1 to 4 remain at 1.33 to 1.44, whereas The value is 1.81, which indicates that the moisture retention is higher than those of Comparative Examples 1 to 4.

  Further, as to the degree of change (average value) in the amount of water after 30 minutes of application of the solution, as shown in Table 2, the values of Comparative Examples 1 to 4 remain at 1.08 to 1.15, whereas for external use in the test The value of the agent is 1.29, which indicates that high moisture retention can be maintained as compared with Comparative Examples 1 to 4.

  Thus, the external preparation (test external preparation) containing pine bark extract, collagen and EGF, compared to Comparative Examples 1 and 3, specifically, a solution containing pine bark extract and collagen, Compared with a solution containing collagen and EGF, high moisture retention can be maintained. From this result, external preparation (external preparation for test) containing pine bark extract, collagen and EGF has better skin moisturizing properties than external preparation containing only collagen as a component to improve skin moisture retention. Can be improved.

(Example 2: Production of cream)
The following ingredients were blended to prepare a cream.
<Cream ingredients> Blending amount (% by weight)
Liquid paraffin 8.00
Dimethicone 5.00
Behenyl alcohol 4.50
Octyldodecanol 1.00
Squalane 2.00
Diisostearyl malate 3.00
Isopropyl palmitate 2.00
Glyceryl stearate 4.00
Hydrolyzed collagen (molecular weight: 100,000) 0.01
Pine bark extract 0.001
Swallow's nest extract 0.10
Glycerin 5.00
1,3 butylene glycol 4.00
Xanthan gum 0.30
Methylparaben 0.30
Pure water balance

  From Example 2 described above, it can be seen that a cream containing a pine bark extract, collagen and swallow's nest extract (EGF-like component) can be produced.

(Example 3: Production of lotion)
The following ingredients were blended to prepare a lotion.
<Ingredients for lotion> Blending amount (% by weight)
PEG-60 hydrogenated castor oil 1.50
Squalane 0.10
Ethanol 8.00
Hydrolyzed collagen (molecular weight: 7000) 0.10
Pine bark extract 0.0001
FGF 0.0001
Methylparaben 0.10
1,3 butylene glycol 8.00
PEG-30 3.00
Glycerin 5.00
Pure water remainder FGF represents Fibroblast Growth Factor, that is, a fibroblast growth (proliferation) factor.

  From Example 3 described above, it can be seen that a lotion containing pine bark extract, collagen and FGF can be produced.

(Example 4: Production of body lotion)
A body lotion was prepared by blending the following ingredients.
<Body lotion ingredients> Formulation amount (wt%)
PEG-60 hydrogenated castor oil 0.20
Jojoba oil 0.05
Ethanol 10.00
Hydrolyzed collagen (molecular weight: 3000) 0.10
Pine bark extract 0.03
TGF 0.001
Methylparaben 0.10
1,3 butylene glycol 3.00
PEG-30 1.00
Glycerin 1.00
Purified water balance TGF indicates transforming growth factor, ie, transforming growth factor.

  From Example 4 described above, it can be seen that a body lotion containing pine bark extract, collagen and TGF can be produced.

(Example 5: Production of face washing powder)
The following ingredients were blended to prepare a face washing powder.
<Ingredients of face washing powder> Blending amount (% by weight)
Lauroyl glutamate Na 25.00
Cocoyl glutamate Na 13.00
Silica 15.00
Maltitol 20.00
Alginate Na 2.00
Hydrolyzed collagen (molecular weight: 10,000) 0.50
Pine bark extract 0.10
IGF 0.10
Corn starch 24.10
Methylparaben 0.20
In addition, IGF has shown Insulin-like Growth Factor, ie, an insulin-like growth factor.

  From Example 5 described above, it can be seen that it is possible to produce a facial cleansing powder containing pine bark extract, collagen and IGF.

(Example 6: Production of foundation)
The following ingredients were blended to prepare a foundation.
<Foundation ingredients> Blending amount (% by weight)
Lauroyllysine 2.85
(Talc / silicic fluoride K) calcined product 45.00
Talc 12.00
Titanium oxide 5.00
Polymethyl methacrylate 11.00
Kaolin 5.00
Silica 2.00
Zinc oxide 10.00
Yellow iron oxide 0.30
Bengala 0.20
Black iron oxide 0.05
Hydrolyzed collagen (molecular weight: 3000) 1.00
Pine bark extract 1.00
HGF 0.50
Methylparaben 0.10
Dimethicone 4.00
In addition, HGF has shown hepatocyte growth factor, ie, hepatocyte growth (proliferation) factor.

  From Example 6 described above, it can be seen that a foundation containing pine bark extract, collagen and HGF can be produced.

(Example 7: Production of soap)
A soap was prepared by blending the following ingredients.
<Soap ingredients> Blending amount (% by weight)
Potash soap base 75.00
Myristic acid 1.50
Palmitic acid 1.00
Lauric acid 1.00
Na chloride 0.50
Glycerin 2.00
Hydrolyzed collagen (molecular weight: 30000) 0.50
Pine bark extract 0.50
EGF 0.001
FGF 0.001
IGF 0.001
TGF 0.001
HGF 0.001
Etidronic acid 4Na 0.05
Pure water balance

  From Example 7 described above, it can be seen that a soap containing pine bark extract, collagen, EGF, FGF, IGF, TGF and HGF can be produced.

  Thus, according to Examples 1-7, about growth factor, it turns out that 0.0001-0.5 weight% is mix | blended with respect to the external preparation total weight. Moreover, about a pine bark extract, it turns out that 0.01-100 weight part is mix | blended with respect to 1 weight part of growth factors. In addition, it can be seen that the pine bark extract is blended in an amount of 0.001 to 1% by weight based on the total weight of the external preparation.

  The present invention has been described above based on the embodiments. However, the present invention is not limited to the above-described embodiments, and various modifications and changes can be easily made without departing from the spirit of the present invention. Can be inferred.

  In the skin moisture content measurement test (Example 1) described above, EGF was used as a growth factor to be blended with the test external preparation, but as a growth factor, it is contained in the swallow's nest as shown in Example 2. Even when an EGF-like component is used, it can be easily inferred that the same result as that of the test external preparation in Example 1 is obtained. This is because the EGF-like component contained in the swallow's nest exhibits the same action as EGF in the body.

  Since the external preparation of the present invention contains a pine bark extract, collagen and a growth factor (especially EGF or EGF-like component), it is compared with an external preparation containing only collagen as a component for improving the moisture retention of the skin. And the moisture retention of skin can be improved more. Therefore, it can be used in industrial fields where it is necessary to further improve the moisture retention of the skin.

Claims (6)

  An external preparation comprising a pine bark extract, collagen and a growth factor.   The external preparation according to claim 1, wherein the growth factor is an EGF (Epideral Growth Factor) component.   The topical preparation according to claim 1, wherein the growth factor is an EGF (Epidmal Growth Factor) -like component contained in a swallow's nest.   The external preparation according to claim 1, wherein the growth factor is blended in an amount of 0.0001 to 0.5% by weight based on the total weight of the external preparation.   The external preparation according to any one of claims 1 to 4, wherein the pine bark extract is blended in an amount of 0.01 to 100 parts by weight with respect to 1 part by weight of the growth factor.   The external preparation according to any one of claims 1 to 5, wherein the pine bark extract is blended in an amount of 0.0001 to 1% by weight based on the total weight of the external preparation.