JP2015127339A - Composition for anti-saccharification - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel composition for anti-saccharification which can be used safely and can be supplied stably.SOLUTION: The invention provides a composition for anti-saccharification containing at least one combination selected from a combination of barley leaf powders or barley leaf extract, and sweet potato stem and leaf powders or sweet potato stem and leaf extract, a combination of barley leaf powders or barley leaf extract, and kale powders or kale extract, and a combination of sweet potato stem and leaf powders or sweet potato stem and leaf extract, and kale powders or kale extract.

Description

  The present invention relates to an anti-glycation composition.

  Protein saccharification reaction where amino group of amino acid or protein reacts irreversibly non-enzymatically with reducing sugar to produce advanced glycation end products (hereinafter abbreviated as AGEs) is called Maillard reaction. As a typical inhibitor, methylguanidine is known.

  In recent years, protein saccharification reactions have occurred in vivo, and it has been found that protein saccharification reactions cause various aging phenomena such as diabetes, arteriosclerosis, and skin sag, wrinkles and dullness associated with aging. It was. Therefore, various glycation inhibitors for proteins that inhibit the production of AGEs have been proposed (Patent Documents 1 and 2).

JP 2002-255813 A JP 2011-102270 A

  An object of the present invention is to provide a new anti-glycation composition that can be used for safety and can be stably supplied.

The present inventor has found that certain functional food ingredients whose safety has been confirmed have an excellent anti-glycation effect and have completed the present invention.
That is, the present invention comprises (a) processed kuzuhana, (b) sweet potato stem powder or extract, (c) kale powder or extract, (d) barley leaf pulverized product or extract, and (e) potato extraction. An anti-glycation composition containing at least one selected from those as a main component is provided.

  The anti-glycation composition of the present invention has an excellent anti-glycation effect on proteins and the like. In addition, since the anti-glycation composition of the present invention is mainly composed of a natural food-derived composition used as a functional food material, it can be used safely and can be supplied stably.

Hereinafter, the present invention will be described in detail.
The anti-glycation composition of the present invention comprises (a) a processed kuzu flower, (b) a sweet potato stem powder or extract, (c) a kale powder or extract, (d) a barley leaf powder or extract, and (e) Contains at least one selected from potato extracts.

(a) Processed Kuzuka In the present invention, the processed Kuzuka refers to a processed Kuzuhana. There are no particular restrictions on the type of kuzu, but examples include Pueraria thomsonii, Pueraria lobata, and Pueraria thunbergiana. Use Pueraria thomsonii. Is preferred.

  In addition, the processed products include, for example, those obtained by performing a treatment such as a drying treatment, a pulverization treatment, and an extraction treatment. As described later, extraction and concentration are performed so that the content of isoflavones components is increased. A processed product obtained by subjecting to a treatment such as fractionation, separation and purification is preferred.

  The dried katsuhana product can be obtained by drying kuzuka, preferably katsuka at the stage of cocoon, by sun drying, hot air drying, or the like. The moisture content is preferably dried to 10% by mass or less. ,

  The processed product of Kuzuka can be obtained by pulverizing a dried product of Kuzuka, and the pulverization can be performed by a method commonly used by those skilled in the art, for example, a ball mill, a hammer mill, a roller mill, or the like. In addition, the crushed katsuka product can be obtained by crushing the collected kuzuka using a mass collider, slicer, comitolol, etc. to obtain a kuzu flower crushed product, and drying this kuzuka crushed product. Also good.

  The katsuhana extraction processed product is extracted by adding a solvent to, for example, raw kuzuka or dried kuzuka, crushed or non-crushed, preferably crushed such as dried kuzuka, It can be obtained by collecting the extract by centrifugation or filtration.

  A specific method for obtaining the processed kuzuhana product can be, for example, a method described in Japanese Patent Application Laid-Open No. 2008-214191.

  The processed kuzuhana product usually contains 0.01 to 50% by mass, preferably 0.1 to 50% by mass, in terms of dry matter, of isoflavones, but is further subjected to a treatment for increasing the content of isoflavones. Those that have been prepared can also be preferably used.

  As a treatment for increasing the content of isoflavones, extraction with a water-containing organic solvent or the like, fractionation by column chromatography or the like may be performed. For example, the content of isoflavones can be increased by subjecting the above-described kuzuka-treated product to extraction with methanol or water-containing methanol, preferably water-containing methanol containing 0.1 to 99.9% methanol. Thus, typically, an isoflavone-containing composition containing 20 to 100% by mass, preferably 40 to 100% by mass, of isoflavones in terms of dry matter can be obtained.

  The processed kuzuhana contains a lot of unique isoflavones. Specific examples include Tectorigenin, Tectorigenin 7-O-xylosylglucoside, 6-hydroxygenistein, 6-hydroxygenistein-6,7-di-O-glucoside, Tectoridin, etc. is there. On the other hand, it is known that genistein, daidzein or glycosides thereof, which are contained in a large amount in plants such as soybeans, are hardly contained in kuzuka. In addition, the isoflavones contained in the processed Kuzuka product can be confirmed quantitatively or qualitatively by HPLC analysis using these as preparations.

   As the processed kuzuhana product, a commercially available product can be used. For example, “Kuzunohana Extract” manufactured by Toyo Shinyaku Co., Ltd. can be used.

(b) Sweet potato stem powder or extract In the present invention, the sweet potato stem powder is a product obtained by drying and pulverizing a sweet potato stem leaf (that is, a sweet potato stem and / or leaf), or by drying and pulverizing a squeezed juice of a sweet potato stem leaf. The sweet potato stem and leaf extract is an extract obtained from sweet potato stem and leaf or a dried product thereof.

  Here, sweet potato refers to a plant belonging to the convolvulaceae family and is generally called sweet potato. The variety of sweet potato is not particularly limited. For example, varieties such as Suiou, Joy White, Koganesengan, Shiroyutaka, Satsuma Musti, Ayamurasaki and the like can be mentioned. Of these, it is preferable to use a high polyphenol content. Suio is suitable for productivity because it has a characteristic that even after harvesting the stems and leaves, young shoots of sweet potato are regenerated from the tip of the same stem.

  As the sweet potato stalks and leaves, it is preferable to use the stems or leaves that are exposed from the ground during the cultivation of sweet potatoes. Preferably, sweet potato foliage that has grown out of the ground from the outside by 5 cm or more, more preferably 10 cm or more, and even more preferably 20 cm or more is preferable. Further, the length from the position where the sweet potato stalks come out of the ground to the tip of the sweet potato stalks and leaves is preferably 300 cm or less, more preferably 200 cm or less, and even more preferably 150 cm or less.

  Further, as the sweet potato stalks and leaves, the tip part of the sweet potato stalks and leaves (“sweet potato young stalks and leaves”) is preferable. As the sweet shoots of sweet potatoes, the shoots and leaves of a portion within 60 cm from the tip of the sweet potato shoots are preferably used. The sweet potato leaves are easy to process because the plants themselves are soft. Furthermore, when the young shoots of sweet potato stems and leaves are dried powder, they have a good touch and can be easily used for various foods.

  In obtaining the sweet potato stalks and leaves, it is preferable to heat-treat the sweet potato stalks and leaves in order to stabilize the quality by inactivating the enzymes in the sweet potato stems and leaves and to prevent the sweet potato stems and leaves from fading. The heating temperature in the heat treatment is preferably higher than 80 ° C, more preferably 85 ° C or higher, and further preferably 90 ° C or higher. The heating time in the heat treatment is preferably 10 minutes or less, more preferably 5 minutes or less, still more preferably 3 minutes or less, and particularly preferably 10 seconds to 1 minute.

  As a specific method for the heat treatment, for example, a method described in JP-A-2006-306852 can be used.

  On the other hand, the sweet potato stalks and leaves extract is obtained by performing an extraction process on the raw stalks and leaves of sweet potato leaves or at least one of the heat treatment, drying process, powdering process, pressing process and the like. The extraction process can be performed, for example, by the method described in WO2005 / 112665.

  As the sweet potato stalks and leaves, commercially available ones can be used. For example, a commercially available sweet potato stalks and leaves made from the sweet potato variety "Suio" can be used.

(c) Kale powder or extract In the present invention, kale powder is a product obtained by drying and pulverizing the leaves and / or stems of the cruciferous plant, or drying and pulverizing the slurry or juice of kale. The kale extract is an extract obtained from kale or its dried product.

Here, as a variety of kale, various types of kale such as kitchen kale, tree kale, bush kale, mallow kale, collard, and green leaf kanran can be used.
Moreover, in this invention, any part of the leaf part of a kale and a stem part is applicable.

  The kale is preferably dried and extracted immediately after harvesting. When time is required for processing, it is preferably stored by storage means commonly used by those skilled in the art, such as low-temperature storage, in order to prevent deterioration of kale.

  A specific method for obtaining a kale powder or a kale extract can be performed, for example, by a method for obtaining a processed kale described in JP-A-2007-99664.

  As the kale powder, a commercially available one can be used, for example, a kale made by Toyo Shinyaku Co., Ltd. can be used.

(d) Barley leaf powder or extract Barley leaf powder is obtained by drying and pulverizing the leaves of barley (Hordeum vulgare L.), or by squeezing and pulverizing barley leaf juice. The barley leaf extract is an extract obtained from barley leaves or a dried product thereof.

The barley variety is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include Nijo barley, Shijo barley, Rojo barley, and bare barley.
Moreover, as barley leaves, it is preferable to use young leaves harvested from the start of tilling to the heading start (the height is about 20 to 40 cm).

  In addition, the barley leaves are washed with fresh leaves and cut as necessary.For example, as in the method for producing barley young leaves described in JP-A-2007-217479, hot water or heated steam is used to prevent alteration. It is preferable to perform a blanching process or a microwave process to be processed, followed by a drying process and a pulverizing process. You may perform based on the powder processing as described in patent 3428956 gazette, patent 3277181 gazette, etc.

  As the barley leaf powder, commercially available products can be used, for example, barley young leaf powder manufactured by Toyo Shinyaku Co., Ltd. can be used.

(e) Potato extract A potato extract is an extract obtained from a potato tuber. As potato varieties, for example, baron rice cake, make-in, kitakari, toya, toyoshiro, inca medusa, digima, tokachi koma and the like can be used.

There is no special restriction | limiting in the manufacturing method of a potato extract, It can be set as the method of using an extraction solvent which can be normally used in manufacture of a foodstuff, You may add manufacturing adjuvants. Further, the extraction method may be a method of recovering the juice by squeezing. Further, the obtained extract can be used after being dried by a conventional method.
As a drying method, a method of adding an excipient and drying by spray drying or the like may be used.

As a more specific embodiment of the method for producing a potato extract, first, an edible portion (tuber) of potato is pulverized, an acid is added to the obtained juice and the pH is adjusted to be acidic, and then heated. Perform solvent extraction at The acid added here generally includes hydrochloric acid and the like. The amount or concentration of the acid to be added is appropriately set in such an amount or concentration that the pH is generally 2 to 5, preferably 3 to 4.
The solvent extraction is generally performed at 70 to 90 ° C. for 10 to 60 minutes, preferably at 75 to 85 ° C. for 10 to 20 minutes.

  After solvent extraction, the precipitate or insoluble fraction is removed by an appropriate separation means such as centrifugation or filtration, and the supernatant or soluble fraction is recovered. Furthermore, the same extraction process can be performed again on the precipitate or insoluble fraction after extraction, and the supernatant or soluble fraction can be recovered.

  From the collected supernatant or soluble fraction, a fraction having a molecular weight of 10,000 or more is separated and collected by membrane treatment, and then caustic soda is added to the cooled extract to adjust the pH to neutral or in the vicinity thereof. It is preferable. In this way, a potato extract is obtained. Examples of membrane treatment for molecular weight fractionation include ultrafiltration membrane treatment and gel filtration membrane treatment, with ultrafiltration membrane treatment being preferred.

  As a potato extract, what is marketed can be used, for example, "Potein" by Toyo Shinyaku Co., Ltd. can be used.

  The anti-glycation composition of the present invention contains at least one of the above-mentioned components (a) to (e), and in particular, (b) sweet potato stem powder or extract, (c) kale powder or extraction The product and (e) barley leaf powder or extract are preferably blended in combination of two or more selected from these. Thereby, an anti-glycation effect can be heightened compared with the case where it mix | blends independently.

  The anti-glycation composition of the present invention can be blended with various components such as an antioxidant, an ultraviolet ray inhibitor, a surfactant, an excipient, and a coloring material as long as the effects of the present invention are not impaired. .

  In addition, since the components (a) to (e) as the main components of the anti-glycation composition of the present invention are each used as a food material or a cosmetic material, the anti-glycation inhibitor of the present invention. The administration form can be oral administration or parenteral administration such as transdermal administration.

  In the case of oral administration, the anti-glycation composition of the present invention is added with a pharmaceutically acceptable base or carrier, if necessary, by a known formulation method, for example, capsules such as hard capsules and soft capsules, It can be administered in the form of tablets, granules, pills, powders, liquids, powders, jellies, candy, pastes, and the like.

  Moreover, when the components (a) to (e) as the main components are the ends of flowers or stems and leaves, they may be packaged in a tea bag shape and leached into hot water before drinking. In addition, the dosage form may be dissolved in water, hot water, milk or the like, or added to food or drink.

  A nutritional component can also be mix | blended with the composition for anti-glycation of this invention. Nutritional components include, for example, vitamins such as ascorbic acid, tocopherol, riboflavin, β-carotene, folic acid and biotin; minerals such as calcium, magnesium, selenium and iron; sulfur-containing compounds contained in taurine and garlic; hesperidin Flavonoids and flavonoids such as quercetin; dietary fibers such as indigestible dextrin, alginic acid, chitin, chitosan and guar gum; proteins such as soy protein and collagen; peptides; amino acids; animals such as milk fat, lard, beef tallow and fish oil Vegetable oils such as soybean oil and rapeseed oil; fruits such as oranges, lemons, grapefruits, strawberries and their juices; royal jelly, propolis, honey, reduced maltose, lactose, sugar alcohol, liquid sugar, seasonings, etc. .

  On the other hand, when the anti-glycation composition of the present invention is administered parenterally, the anti-glycation composition of the present invention comprises ointments, creams, gels, lotions, emulsions, packs, poultices, bath preparations, eye drops. In the form of nasal drops, it can be used as an external preparation.

  In addition, the anti-glycation composition of the present invention can be blended into hair cosmetics such as skin cosmetics, shampoos, rinses, and conditioners, and in that case, it is used together with base materials and additives usually used in cosmetics. be able to. Furthermore, various auxiliary agents can be added for the purpose of enhancing or supplementing the function of the external preparation of the present invention. For example, the base includes glycerol, ethanol, paraben, or butylene glycol.

  Examples of the additives include excipients (silicon-based polymers), fragrances, pigments, preservatives (parabens, etc.), thickeners (silicon-based polymers, acrylic polymers, carboxyvinyl polymers, etc.), chelating agents (EDTA). Etc.), sweeteners (such as sucralose), refreshing agents (such as menthol), antiseptic / antifungal agents (such as phenoxyethanol) and the like.

  Examples of the auxiliary agent include other medicinal ingredients and other oils (unsaturated fatty acids such as linoleic acid, linolenic acid, palmitic acid, DHA, EPA, and derivatives thereof, linseed oil, coconut oil, jojoba oil, olive oil, Oils extracted from animals and plants such as squalane, squalene, horse oil, rice bran oil, castor oil and derivatives thereof, moisturizer (collagen or its degradation products, collagen-like peptides such as carrot extract, soy peptide, amino acid, Mucopolysaccharides such as hyaluronic acid, amino acids such as chondroitin, saccharides such as trehalose, seaweed, water-soluble dietary fiber such as alginic acid, glucomannan, and pectin, phospholipids, etc., surfactants (lecithin, fatty acid esters, amino acid derivatives) UV absorbers (such as zinc oxide and titanium oxide), absorption Susumu agents.

  In using the composition for anti-anti-glycation of the present invention, the dose of each of the components (a) to (e) can be appropriately determined according to the combination of these components and the form of the composition. Usually, it is preferably 0.00001% by mass or more, more preferably 0.001% by mass or more, in terms of dry mass in the administration.

  Hereinafter, the present invention will be specifically described based on examples.

Example 1 (Anti-glycation effect of processed Kuzuhana)
As a test substance of Example 1, a kuzu flower processed product (“Kuzu no Hana Extract” manufactured by Toyo Shinyaku Co., Ltd., isoflavone content 20%) is prepared, and as a test substance of Comparative Example 1, a soybean extract (isoflavone content 40%) Aminoguanidine (aminoguanidine hydrochloride, Cayman Chemical Co.), which is a typical anti-glycation agent, was prepared as a test substance (positive control) of the reference example.

  Meanwhile, a phosphate buffer powder (1/15 mol / l pH 7.2) (Wako Pure Chemical Industries, Ltd.) was dissolved in distilled water to prepare a 67 mM phosphate buffer (hereinafter abbreviated as 67 mM PB). In addition, D (+) glucose (Kanto Chemical Co., Ltd.) was dissolved in 67 mM PB to prepare a 200 mg / mL glucose solution, and albumin (bovine serum-derived corn fraction V, pH 7.0, for biochemistry) (Japanese) Optical Pure Chemical Industries, Ltd. (hereinafter abbreviated as BSA) was dissolved in 67 mM PB to prepare 40 mg / mL BSA.

  Prepare a test substance solution by dissolving predetermined amounts of the test substances of Example 1, Comparative Example 1 and Reference Example 1 with 67 mM PB, respectively, and mix the test substance solution, glucose solution, BSA solution and PB in the proportions shown in Table 1. Test solutions and controls were prepared. Table 2 shows the test substance solution and the test substance concentration in the test solution.

  The test solution or control was incubated at 60 ° C. for 48 hours, and the fluorescence intensity at 440 nm when the test solution after incubation was excited at 370 nm was measured with a spectrofluorometer, and the AGEs production inhibition rate (%) was calculated by the following formula. Calculated.

AGE generation inhibition rate (%)
= (1-[(Sample _test −Sample _blank ) / (Control _test −Control _blank )]) × 100
(In the formula, Sample _test : Fluorescence intensity of test solution (test)
Sample _blank : Fluorescence intensity of test solution (blank)
Control _test : Fluorescence intensity of control (test)
Control _blank : Fluorescence intensity of control (blank)
The results are shown in Table 2.

  From Table 2, it can be seen that the processed kuzuhana product has a higher anti-glycation effect than the soybean extract whose anti-glycation effect is known.

Example 2 (Anti-glycation effect of potato extract)
A potato extract (“Potein” manufactured by Toyo Shinyaku Co., Ltd.) was prepared as a test substance of Example 2, and aminoguanidine was prepared as a test substance of Reference Example in the same manner as in Example 1.
In the same manner as in Example 1, a test substance solution having a test substance concentration shown in Table 3 was prepared, and a test solution with the test substance having the concentration shown in Table 3 was prepared and incubated. AGEs The production inhibition rate (%) was calculated. The results are shown in Table 3.
From Table 3, it can be seen that the potato extract has an anti-glycation effect.

Examples 3 to 5 (Anti-glycation effect of sweet potato stem powder, barley leaf powder, kale powder)
Examples of test substances of Examples 3, 4 and 5 are sugar cane stem powder (Suyo (manufactured by Toyo Shinyaku) (green powder)), barley leaf powder (barley young (manufactured by Toyo Shinyaku) (green powder)), kale powder (manufactured by Toyo Shinyaku) Kale (green powder) was prepared, and aminoguanidine was prepared in the same manner as in Example 1 as the test substance of the reference example.

In the same manner as in Example 1, a test substance solution having the test substance concentrations shown in Tables 4 to 6 was prepared, and a test solution with the test substance having the concentration shown in the table was prepared and incubated. The AGE generation inhibition rate (%) was calculated.
The results are shown in Tables 4-6.

  From Table 4 to Table 6, it can be seen that sweet potato stem powder, barley leaf powder, and kale powder all have an anti-glycation effect.

Example 6 (combination of sweet potato stem powder and kale powder)
Using test substances of Examples 3 and 5 mixed at a ratio of 1: 1, a test substance solution having the concentrations shown in Table 6 was prepared in the same manner as in Example 1, and the test substances were used in the same table. A test solution having a concentration of 2 was prepared, incubated, and the inhibition rate (%) of AGE generation was calculated. The results are shown in Table 7.
From Table 7, it can be seen that the anti-glycation action becomes stronger when used in combination than when the sweet potato stem powder and kale powder are used alone.

Example 7 (combination of sweet potato stem powder and barley leaf powder)
A test substance solution having the concentrations shown in Table 8 was prepared in the same manner as in Example 1 using a mixture of the test substances of Examples 3 and 4 at 1: 1, and the test substance was used in the same table. A test solution having a concentration of 2 was prepared, incubated, and the inhibition rate (%) of AGE generation was calculated. The results are shown in Table 8.
From Table 8, it can be seen that the anti-glycation action is strengthened when used in combination rather than using sugar cane stem powder and barley leaf powder individually.

Example 8 (combination of kale powder and barley leaf powder)
Using test substances of Examples 4 and 5 mixed at a ratio of 1: 1, a test substance solution having the concentrations shown in Table 8 was prepared in the same manner as in Example 1, and the test substances were used in the same table. A test solution having a concentration of 2 was prepared, incubated, and the inhibition rate (%) of AGE generation was calculated. The results are shown in Table 9.
From Table 9, it can be seen that anti-glycation action is enhanced when kale powder and barley leaf powder are used in combination rather than individually.

Claims (2)

An anti-glycation composition comprising one or more selected from kale powder or kale extract, barley leaf powder or barley leaf extract, sweet potato leaf powder or sweet potato leaf extract, and potato extract.
  An anti-glycation composition comprising two or more selected from kale powder or kale extract, barley leaf powder or barley leaf extract, sweet potato stalk powder or sweet potato shoot extract, and potato extract.