KR20120024262A - Compositions having antioxidant activity which comprise mixed extracts of natural substances - Google Patents

Abstract

PURPOSE: A composition containing natural mixture extract is provided to ensure antioxidation and to prevent or treat aging. CONSTITUTION: A composition with antioxidation contains extract of Zinnia elegans stems, Cryptomeria japonica stem bark, Pyrus ussuriensis stem bark, Crataegus pinnatifida stem bark, Acer mono Max. stem bark, Castanea crenata leaf, Cornus kousa leaf, and Eriobotrya japonica leaf. The mixture extract is prepared using water, C1-C4 alcohol, or a mixture thereof. A food, cosmetic product, medicinal product or animal fed contains the composition.

Description

A composition having antioxidant activity, including a mixed extract of a natural product {COMPOSITIONS HAVING ANTIOXIDANT ACTIVITY WHICH COMPRISE MIXED EXTRACTS OF NATURAL SUBSTANCES}

The present invention relates to a composition having an antioxidant activity, comprising a mixed extract of natural products, more specifically crape myrtle stem, cedar stem bark, hawthorn stem bark, hawthorn stem bark, cypress stem bark, chestnut leaf, wildberry It relates to a composition having an antioxidant activity, comprising two or more mixed extracts selected from the group consisting of tree leaves and loquat leaves.

Since free radicals are chemically very unstable compounds, they have a strong tendency to take electrons from other compounds around them and become stable compounds. As a result, molecules around free radicals easily lose electrons, causing oxidative damage, as well as a series of reactions that themselves become free radicals and attack surrounding compounds. These free radicals are naturally produced in the human body through life activities. Representative free radicals include superoxide radicals derived from oxygen (ground molecular triplet oxygen, which is a stable molecular state) which is essential for the production of energy necessary for life support. superoxide radical (O ₂ ^-) in reactivity, such as a), hydroxyl radicals (? hydroxyl radical (HO)) , hydrogen peroxide _{(hydrogen peroxide (H 2 O 2} )) , and singlet oxygen _{^{(singlet oxygen (1 O 2)}} ) so There is a large free radical, reactive oxygen species (ROS). Oxygen species produced in this way oxidatively damage fats, proteins and genes that make up the human body, and cause aging, brain diseases such as stroke, Parkinson's disease, heart diseases, ischemia, arteriosclerosis, skin damage, inflammation, It causes various diseases such as rheumatism and autoimmune diseases. In human body, superoxide dismutase (SOD), peroxidase, catalase as a means of protecting the human body from these active oxygen species. And antioxidant enzyme systems such as glutathione peroxidase. However, in recent years, the balance of our body has been broken down by various environmental factors such as chemicals, food additives, smoking, drinking, ultraviolet rays and mental stress caused by pollution and environmental pollution. The generation of reactive oxygen species is beyond the ability of the human body's self-antioxidant protection system is increasing the rate of occurrence and aging of various diseases.

Antioxidant activity refers to the ability to prevent excessive generation of free radicals in vivo as well as to prevent oxidative phenomena that cause irreparable damage to cells. Substances having such antioxidant activity are called antioxidants and they are divided into synthetic antioxidants synthesized artificially and natural antioxidants present in nature. The synthetic antioxidants developed so far include butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and nordihydroguaiaretic acid (NDGA), and natural antioxidants include superoxide dismutase, peroxidase, catalase and glutathione peroxidase. Antioxidant enzymes and non-enzymatic antioxidants such as ascorbic acid (vitamin C), tocopherol (vitamin E) and carotenoids. However, synthetic antioxidants are not easily destroyed when heated due to weak temperature, and there is a lack of active oxygen scavenging effect in the living body and can cause various diseases such as allergy or cancer in the human body. have. Therefore, attempts are being made to find antioxidants from natural products that have high antioxidant activity and are harmless to the human body. Such natural antioxidants are known to have effects such as plant extracts such as green tea, prickly pear, leather, persimmon, persimmon leaf, golden, nectar, brier, dried, dandelion, safflower, and lumber. Food additives and cosmetic compositions using them are known. And pharmaceutical compositions are being researched and developed. Despite these efforts, however, there are countless antioxidants that are still unknown in nature, and thus, the need for research and development of natural antioxidant materials with better antioxidant activity is increasing day by day.

Zinnia elegans is an annual plant of the Asteraceae. Flowers bloom from the end of stems or axillas in July-October and bloom in various colors including red, yellow, violet, white, and pale yellow. . Cedar (Cryptomeria japonica) is an evergreen tree of the larch family, and is used for materials of construction, civil engineering, ships, furniture, windbreak, and forest greening. Pyrus ussuriensis is a deciduous broad-leaved arborescent of the family Rosaceae, whose fruit is edible. Hawthorn (Crataegus pinnatifida) is a deciduous broad-leaved arborescent of the Rosaceae family, which inhabits mountain areas throughout the country, and its fruit is used for food and medicinal purposes. Acer mono Max. Is a deciduous broad-leaved shrub of the Maple family. The sap is edible and the bark is used for medicinal purposes. The chestnut tree (Castanea crenata) is a deciduous broad-leaved arboreous tree of the oak family, which is edible or medicinal. Cornus kousa is a deciduous arborescent of the dogwood family, whose fruit is edible. Loquat tree (Eriobotrya japonica) is an evergreen broad-leaved arborescent of the family Rosaceae, whose fruit is edible and its leaves are used for medicinal purposes. However, few have been reported to have good antioxidant activity at certain parts of these plants, such as crape myrtle, cedar bark, hawthorn bark, hawthorn bark, cypress bark, chestnut leaves, cornel leaves and loquat leaves. None of these mixed extracts have been reported to have antioxidant activity.

The inventors of the present invention, while developing an antioxidant composition derived from natural products, confirmed that the extract extracted by mixing two or more of the natural products has superior antioxidant activity compared to trolox, a powerful antioxidant, and completed the present invention.

Accordingly, it is an object of the present invention to provide a composition having antioxidant activity, comprising a mixed extract of natural products.

According to the above object, the present invention is a mixture of two or more selected from the group consisting of crape myrtle stem, cedar stem bark, hawthorn stem bark, hawthorn stem bark, cypress stem bark, chestnut leaf, cornel leaf and loquat leaf It provides a composition having an antioxidant activity, including an extract.

The present invention also provides a food, cosmetics, pharmaceuticals and animal feed comprising the composition.

Since the mixed extract of the present invention is very excellent in antioxidant activity, it can be usefully used for preventing or treating a pharmaceutical composition for aging prevention, functional food composition for preventing or improving aging, functional cosmetics or animal feed.

The present invention comprises two or more mixed extracts selected from the group consisting of crape myrtle stem, cedar stem bark, hawthorn stem bark, hawthorn stem bark, cypress stem bark, chestnut leaf, cornel leaf and loquat leaf, Provided are compositions having antioxidant activity.

The extract may be prepared by conventional extraction methods known in the art, such as ultrasonic extraction, filtration and reflux extraction. Preferably, the extract is prepared by extracting a mixture of natural products according to the invention with water, C ₁ ~ C ₄ alcohol or a mixed solvent thereof, or more preferably with water, 50% ethanol or 50% methanol Can be. Alternatively, a mixed extract may be prepared by mixing extracts obtained by extracting each natural product by the above method.

For example, two or more selected from the group consisting of dried and finely cut crape myrtle stem, cedar stem bark, hawthorn stem bark, hawthorn stem bark, cypress stem bark, chestnut leaf, cornel leaf and loquat leaf Then, it can be extracted by repeating the extraction by refluxing after soaking in water, 50% ethanol or 50% methanol. Preferably, the immersion liquid of the dried and shredded natural product sample may be ultrasonically treated, followed by extraction by reflux extraction. The ultrasonic conditions may be treated with 15 to 30 kHz, preferably 20 kHz, 50 to 200 watts / liter, preferably 100 watts / liter, 15 to 60 minutes, preferably 30 minutes , Reflux extraction may be repeated 10 times for 1 hour at 40 to 100 ℃. The amount and time of the extraction solvent may vary depending on the experimental conditions, it can be carried out by those skilled in the art freely modified. Thereafter, the process of filtration, concentration under reduced pressure, or freeze drying may be further performed.

In one embodiment, the mixed extract may be a mixed extract of zinnia stem and cornel leaves. In another embodiment, the mixed extract may be a mixed extract of cedar stem bark, hawthorn stem bark and hawthorn stem bark. In another embodiment, the mixed extract may be a mixed extract of cedar stem bark, elder bark stem and loquat leaf. In another embodiment, the mixed extract may be a mixed extract of the crape myrtle stem and cedar stem bark. In another embodiment, the mixed extract may be a mixed extract of cornel leaves, wild berry stem bark and loquat leaves. In another embodiment, the mixed extract may be a mixed extract of hawthorn stem bark, hawthorn stem bark, and cypress stem bark. In another embodiment, the mixed extract may be a mixed extract of cornel leaves, hawthorn stem bark, crocus stem bark and chestnut leaves. Those skilled in the art can combine the natural products in various components and ratios to produce mixed extracts, all of which are within the scope of the present invention.

The mixed extract according to the embodiment may be obtained by mixing 30 to 70 parts by weight, preferably 50 parts by weight, and 30 to 70 parts by weight, preferably 50 parts by weight of wild berry stem. The mixed extract according to the other embodiment is 30 to 70 parts by weight, preferably 50 parts by weight, 15 to 35 parts by weight of hawthorn stem bark, preferably 25 parts by weight and 15 to 35 parts by weight of hawthorn stem bark Part, preferably 25 parts by weight can be obtained by mixing. The mixed extract according to another embodiment is 35 to 45 parts by weight, preferably 40 parts by weight of cedar stem bark, 35 to 45 parts by weight, preferably 40 parts by weight and 10 to 30 parts by weight of loquat leaf Part, preferably 20 parts by weight can be obtained by mixing. The mixed extract according to another embodiment may be obtained by mixing 30 to 70 parts by weight, preferably 50 parts by weight, and 30 to 70 parts by weight, preferably 50 parts by weight of cedar stalk stem. Mixed extract according to another embodiment is 15 to 35 parts by weight of cornel leaves, preferably 25 parts by weight, 30 to 70 parts by weight of wild berry stem bark, preferably 50 parts by weight and 15 to 35 parts by weight of loquat leaves Part, preferably 25 parts by weight can be obtained by mixing. The mixed extract according to another embodiment is 15 to 35 parts by weight, preferably 25 parts by weight, hawthorn stem bark 15 to 35 parts by weight, preferably 25 parts by weight and hawthorn stem bark 30 to 20% 70 parts by weight, preferably 50 parts by weight can be obtained by mixing and extracting. The mixed extract according to another embodiment is 15 to 25 parts by weight, preferably 20 parts by weight, wild berry stem bark 25 to 35 parts by weight, preferably 30 parts by weight, hawthorn stem bark 25-35 It can be obtained by mixing by weight, preferably 30 parts by weight and 15 to 25 parts by weight, preferably 20 parts by weight of chestnut leaves.

The natural product mixed extract according to the present invention has excellent free radicals compared to trolox (a water-soluble derivative of vitamin E), which is a powerful antioxidant as a result of measuring the electron donating ability by DPPH method and the hydroxyl radical scavenging activity by the Fenton reaction. Scavenging activity is shown (see Tables 2 and 3).

Therefore, the mixed extract of natural products according to the present invention can be used in the form of a general antioxidant pharmaceutical preparations. The pharmaceutical preparation may be administered in an oral or parenteral dosage form, and when formulated, it may be prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. which are commonly used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, calcium carbonate in a mixed extract of the natural product of the present invention. Sucrose (Sucrose) or lactose (Lactose), gelatin and the like are mixed and prepared. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The dosage of the mixed extract of the natural product of the present invention varies depending on the weight, age, sex, health condition, diet, time of administration, administration method, excretion rate and severity of the disease of the patient, the daily dosage is natural 0.1 to 100 mg / kg, preferably 30 to 80 mg / kg, more preferably 50 to 60 mg / kg, based on the amount of the mixed extract, can be administered 1-3 times a day.

The extract of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers.

In addition, the present invention, the mixed extract of the natural product may be provided in a food form.

When the mixed extract of the natural product according to the present invention is used as a food additive, the extract may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient to be mixed may be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). Generally, in the preparation of food or beverages, the composition of the present invention is added in an amount of up to 15 parts by weight, preferably up to 10 parts by weight based on the raw materials. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. .

There is no particular limitation on the kind of food. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the present invention contains various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonic acid. Carbonating agents and the like used in beverages. In addition, the extract or fraction of the present invention may contain a flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

In addition, the mixed extract of the natural product according to the present invention may be provided in a cosmetic form.

Cosmetics prepared by containing the mixed extract of natural products according to the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), nonionic vesicles obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase, for example. It may be provided in the form of a dispersant, cream, skin, lotion, powder, ointment, spray or cone stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition to the extracts or fractions of the present invention, the cosmetics may contain fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And any other ingredients that may be used, such as auxiliaries commonly used in the cosmetic field.

Furthermore, the mixed extract of natural products according to the present invention may be provided in the form of animal feed. The animal feed may be in any suitable form, such as dry form, semi-wet form or wet form, which may be a refrigerated or long-term preserved pet food product. The animal feed may contain any carbohydrate source, protein source and lipid source, in addition to the mixed extract of the natural product according to the present invention. The animal feed can be prepared using conventional manufacturing methods well known in the art, namely extrusion cooking, baking and other suitable processing methods. When extruding animal feed, it can usually be provided in kibble form. For wet foods or wet pet foods, they may be provided in the form of meat mimic products. Other methods for the production of chunk type products can also be used for cooking, for example in steam ovens. Alternatively, meat emulsions may be prepared by emulsifying the appropriate meat material, the appropriate gelling agent may be added, and the meat emulsion may be heated and placed in cans or other containers to produce a loaf type product.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in detail, and the scope of the present invention is not limited by these examples.

Example  1: Preparation of Mixed Extract of Crape Myrtle and Cornel Leaves

50 parts by weight of dried (1 × 2 cm) crape myrtle stem and 50 parts by weight of cornel leaves were mixed and immersed in distilled water of 5 times the mixture weight for 24 hours. Then, the mixture was placed in a flask equipped with a reflux apparatus, treated with 100 watts / liter for 30 minutes using ultrasonic waves at 20 kHz, and then subjected to 10 times of ultrasonic reflux extraction, which was extracted while refluxing at 100 ° C. for 1 hour. Repeated. The extract was filtered and concentrated under reduced pressure to prepare a mixed extract A (yield 11.6%).

Example  2: cedar trunk bark, hawthorn trunk bark and Hawthorn  Preparation of Mixed Extract of Stem Bark

50 parts by weight of dried (1 × 2 cm) cedar stem bark, 25 parts by weight of hawthorn stem bark, and 25 parts by weight of hawthorn stem bark were mixed, and then mixed extract B (yield 12.3%) in the same manner as in Example 1. ) Was prepared.

Example  3: Preparation of mixed extracts of cedar trunk bark, cypress trunk bark and loquat leaf

40 parts by weight of dried cedar stem bark (1 × 2 cm), 40 parts by weight of cypress stem bark, and 20 parts by weight of loquat leaf were mixed and mixed extract C (yield 11.5%) in the same manner as in Example 1. Was prepared.

Example  4: Preparation of Mixed Extract of Crape Myrtle and Cedar Stem Bark

50 parts by weight of dried chopped (1 × 2 cm) crape myrtle stem and 50 parts by weight of cedar stem bark were mixed, and a mixed extract D (yield 11.4%) was prepared in the same manner as in Example 1.

Example  5: Preparation of mixed extract of cornel leaves, hawthorn stem bark and loquat leaves

25 parts by weight of dried, shredded (1 × 2 cm) cornel leaves, 50 parts by weight of berry stem bark and 25 parts by weight of loquat leaf, and then mixed extract E (yield 11.0%) in the same manner as in Example 1. Was prepared.

Example  6: hawthorn stem bark, Hawthorn  Preparation of Mixed Extract of Stem Bark and Cypress Bark

25 parts by weight of dried and shredded (1 × 2 cm) hawthorn stem bark, 25 parts by weight of hawthorn stem bark, and 50 parts by weight of buckthorn stem bark, and then mixed extract F (yield 11.7) in the same manner as in Example 1. %) Was prepared.

Example  7: Manufacture of a mixed extract of cornel leaves, hawthorn stem bark, elder bark bark and chestnut leaves

20 parts by weight of dried and shredded (1 × 2 cm) cornel leaves, 30 parts by weight of hawthorn stem bark, 30 parts by weight of buckthorn stem bark and 20 parts by weight of chestnut leaf, followed by mixing in the same manner as in Example 1 Extract G (yield 11.3%) was prepared.

The composition for preparing the mixed extract is summarized in Table 1 below.

Experimental Example  One: DPPH  freedom Radical  Scavenging activity measurement

In order to examine the antioxidant activity of the mixed extract obtained in Examples 1 to 7, free radical scavenging activity was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl). Specifically, the mixed extracts of Examples 1 to 7 were dissolved in distilled water by concentration, and then 100 μL of the sample was added to 4.9 mL of DPPH solution (200 μM) dissolved in 100% ethanol and mixed well. Then, after reacting at 25 ° C. for 20 minutes, the absorbance of the reaction solution was measured at 525 nm. A 100% ethanol solution was used as a control and a 1% trolox solution, a standard antioxidant, was used as a comparative group. DPPH solution was prepared and used on the day of testing. From the absorbance results of each sample, DPPH free radical scavenging activity (%) was calculated using the following equation.

[Equation 1]

DPPH free radical scavenging activity (%) = 100 × [1- (absorbance of sample / absorbance of control)]

Table 2 shows the free radical scavenging activity of each mixed extract as the sample concentration (IC ₅₀ , μg / ml) required for the DPPH free radical scavenging activity to be 50%.

sample IC ₅₀ (μg / ml) Mixed Extract A (Example 1) 7 Mixed Extract B (Example 2) 7 Mixed Extract C (Example 3) 7 Mixed Extract D (Example 4) 7 Mixed Extract E (Example 5) 8 Mixed Extract F (Example 6) 7 Mixed Extract G (Example 7) 8 Comparative Group (Trolrox) 15

As shown in Table 2, the mixed extracts of the present invention showed about 2 times higher free radical scavenging activity than trolox, which is a water-soluble derivative of tocopherol (vitamin E), a powerful antioxidant.

Experimental Example 2: Test for hydroxyl radical scavenging activity

In order to examine the antioxidant activity of the mixed extract obtained in Examples 1 to 7, the hydroxyl radical scavenging activity was measured using a Fenton reaction. Specifically, 0.2 mL of the mixed extract sample and 0.2 mL of 50 mM FeSO ₄ H ₂ O solution, 0.2 mL of 50 mM EDTA solution, 50 mM 2, which were dissolved in 1 mL of 100 mM phosphate buffer (pH 7.4) by concentration in distilled water. 0.2 mL of deoxyribose solution and 50 mM hydrogen peroxide 0.2 mL was mixed well and reacted at 37 ° C for 3 hours. The reaction was then stopped by addition of 1 mL of 2.8% trichloroacetic acid (TCA) solution and 1 mL of 1% thiobarbituric acid (TBA) / 50 mM sodium hydroxide (NaOH) for 15 minutes at 100 ° C. After heating, the solution was rapidly cooled and the absorbance of the reaction solution was measured at 530 nm. From the absorbance results of each sample, the hydroxyl (OH) radical scavenging activity (%) was calculated using the following equation.

[Equation 2]

Hydroxyl radical scavenging activity (%) = [1- (A _S -A _b ) / (A _C -A _b )]

Where A _S is the absorbance of the sample, A _C is the absorbance of the distilled water, and A _b is the same method as the sample after stopping the reaction by adding trichloroacetic acid (TCA) solution before adding the sample. Absorbance.

The hydroxyl radical scavenging activity of each mixed extract was shown in Table 3 below with the concentration of the sample (IC ₅₀ , μg / ml) required for the hydroxyl radical scavenging activity to be 50%.

sample IC ₅₀ (μg / ml) Mixed Extract A (Example 1) 63 Mixed Extract B (Example 2) 69 Mixed Extract C (Example 3) 65 Mixed Extract D (Example 4) 67 Mixed Extract E (Example 5) 67 Mixed Extract F (Example 6) 66 Mixed Extract G (Example 7) 65 Comparative Group (Trolrox) 90

As shown in Table 3, the mixed extracts of the present invention exhibited about 1.3 to 1.4 times higher hydroxyl radical scavenging activity than trolox, which is a water-soluble derivative of the powerful antioxidant tocopherol (vitamin E).

The results show that the mixed extract according to the present invention has very high antioxidant activity.

Claims (14)

Antioxidant activity comprising at least two mixed extracts selected from the group consisting of crape myrtle stem, cedar stem bark, hawthorn stem bark, hawthorn stem bark, cypress stem bark, chestnut leaf, cornel leaf and loquat leaf Having a composition.
The composition of claim 1, wherein the mixed extract is extracted using water, C ₁ -C ₄ alcohol, or a mixed solvent thereof.
The composition of claim 1, wherein the mixed extract is prepared by immersing the mixture in an extraction solvent, treating with ultrasound, and then refluxing the mixture.
The composition according to claim 1, wherein the mixed extract is a mixed extract of 30 to 70 parts by weight of crape myrtle stem and 30 to 70 parts by weight of cornel leaves.
The composition according to claim 1, wherein the mixed extract is a mixed extract of 30 to 70 parts by weight of cedar stem bark, 15 to 35 parts by weight of hawthorn stem bark and 15 to 35 parts by weight of hawthorn stem bark.
The composition according to claim 1, wherein the mixed extract is a mixed extract of 35 to 45 parts by weight of cedar stem bark, 35 to 45 parts by weight of cypress stem bark and 10 to 30 parts by weight of loquat leaf.
The composition of claim 1, wherein the mixed extract is a mixed extract of 30 to 70 parts by weight of crape myrtle stem and 30 to 70 parts by weight of cedar stem bark.
The composition according to claim 1, wherein the mixed extract is a mixed extract of 15 to 35 parts by weight of cornel leaves, 30 to 70 parts by weight of wild berry stem bark and 15 to 35 parts by weight of loquat leaves.
The composition according to claim 1, wherein the mixed extract is a mixed extract of 15 to 35 parts by weight of hawthorn stem bark, 15 to 35 parts by weight of hawthorn stem bark and 30 to 70 parts by weight of cypress stem bark.
According to claim 1, wherein the mixed extract is 15 to 25 parts by weight of the cornel leaves, 25 to 35 parts by weight of the bark berry stem bark, 25 to 35 parts by weight of the buckthorn stem bark and 15 to 25 parts by weight of chestnut leaves Characterized in that the composition.
Food comprising the composition of claim 1.
A cosmetic comprising the composition of claim 1.
A pharmaceutical product comprising the composition of claim 1.
Animal feed comprising the composition of claim 1.