KR101771702B1 - Food composition for anti-oxidative activity comprising moringa leaves - Google Patents

Abstract

The present invention relates to a composition for antioxidant, functional cosmetics, feed or feed additive containing Moringa leaf, mash, beta-glucan and menthol, and a pharmaceutical composition for preventing or treating diseases caused by active oxygen. The composition provided by the present invention can be widely used for the development of a variety of functional foods having excellent physiological activity and texture because it exhibits excellent antioxidative activity and low calorie content and can be used for the production of functional food having excellent texture will be.

Description

[0001] The present invention relates to an antioxidative food composition containing moringa leaves,

The present invention relates to a food composition for antioxidation comprising Moringa leaves. More particularly, the present invention relates to a composition for antioxidation comprising Moringa leaf, moss, Beta-Glucan and Rhizome, functional cosmetics, feed or feed additive And a pharmaceutical composition for preventing or treating diseases caused by active oxygen.

Although the human body has a balance of the oxidation promoting substance and the oxidation inhibiting substance, when the balance state is lost due to various factors and is inclined to promote the oxidation, oxidative stress is induced in the living body, Leading to pathological disease. Reactive oxygen species (ROS), which is a direct cause of oxidative stress, are chemically unstable and highly reactive, and can easily react with various biomaterials such as DNA, protein, lipid and carbohydrate. Attacks, causing irreversible damage to cells and tissues, or causing mutations, cytotoxicity, and cancer.

Skin aging by oxidation is due to free radicals, and the theory of free radicals was presented by D. Harman in the mid-1950s. Free radicals are produced by white blood cell phagocytosis, the action of the electron transport system, myeloperoxin (MPO), ultraviolet light, tobacco, normal metabolic processes, stress, pollutants and bacteria during the ATP production process in the mitochondria, When the radicals are left in the human body, various problems such as wrinkle formation, skin cancer, cell death, rheumatoid arthritis, atopic dermatitis, acne and the like are caused through destruction of cells in the body, cutting of connective tissue and induction of cross- . Superoxide dismutase (SOD), Catalase, Vitamin E, Vitamin C, Ubiquinol, etc. are present in the human body to remove free radicals , The body's antioxidant system gradually begins to decline due to age, pollution, ultraviolet rays, stress, etc., and the antioxidant system is disabled, resulting in an increase in free radicals in the body. The increased radical destroys collagen (collagen), elastin, and hyaluronic acid, which are connective tissues of the dermis, causing skin settlement (wrinkles), oxidizing the lipid part of the cell membrane and destroying the cell, , Skin cancer and the like. In addition, this radical participates in a spontaneous oxidation reaction during the melanin formation process, and causes the cause of spots and freckles, and also causes wrinkles.

In addition, reactive oxygen species (ROS) are produced constantly through the oxidation and reduction of oxygen by respiration and immune reactions in mitochondria within the cell. Harmful ROS are usually caused by superoxide dismutase, catalase, glutathione peroxidase, glutamine reductase, vitamin C, vitamin E, uric acid, bilirubin, It is eliminated by the action of the same antioxidant system. However, when the balance of generation and elimination of free radicals is collapsed, oxidative stress occurs, which causes various pathological changes such as inflammation, aging, or cancer. In fact, it has been confirmed that oxidative stress is increased in many clinical diseases. Various studies on new antioxidants have been made to reduce such oxidative stress.

Particularly, researches for antioxidant activity and safety at the same time by searching for antioxidants in natural products are being actively carried out. For example, Japanese Patent Laid-Open Publication No. 2005-160466 discloses tea beverages exhibiting antioxidative activity including dry crumbs of Meringa leaves, Korean Patent Publication No. 2009-0059270 discloses that a large amount of beta-glucan is contained , Korean Patent Registration No. 779855 discloses an extract of buckwheat sprouts showing antioxidative activity obtained by germinating buckwheat seeds, Korean Patent Registration No. 1022705 discloses an antioxidant activity effect An ethanol extract of Ecklonia cava has been disclosed, and Korean Patent Laid-Open Publication No. 2013-0117158 discloses an antioxidative composition comprising a Laustostromia obovolipia extract or a fraction thereof. However, although the active ingredient itself provided by these techniques exhibits excellent antioxidative activity, the antioxidative activity of the active ingredient can be attenuated or removed due to various ingredients contained in the food, There is a problem in that it may not be able to exert the effect. Therefore, development of an effective ingredient capable of stably expressing antioxidant activity even in foods is required.

Under these circumstances, the inventors of the present invention have made extensive efforts to develop an active ingredient capable of stably exhibiting antioxidant activity even when contained in foods. As a result, it has been found that a food composition containing moringa leaves, moth, beta-glucan, It was confirmed that the antioxidative activity was stable, and the present invention was completed.

It is an object of the present invention to provide an antioxidant food composition comprising Moringa leaves, Maize, Beta-Glucan and Rhizome.

Another object of the present invention is to provide a functional cosmetic exhibiting an antioxidative activity including the above-mentioned Moringa leaf, lysimeter, beta-glucan and menthol.

It is still another object of the present invention to provide a feed or feed additive exhibiting antioxidative activity including the above-mentioned Moringa leaf, seedlings, beta-glucan and menthol.

It is still another object of the present invention to provide a pharmaceutical composition for preventing or treating diseases caused by active oxygen, including Moringa leaf, moth, beta-glucan, and menthol.

As one embodiment for achieving the above-mentioned object, the present invention provides an antioxidant food composition comprising a Moringa leaf, a marigold, a beta-glucan, a menthol or a combination thereof.

The meringue leaf, mesophyll or phloem which may be contained in the antioxidative food composition may be all or part of them. When all of them are used, it is preferable to dry the moringa leaf, Powder, and may be an extract or a fraction thereof when a part thereof is used.

The term "extract " of the present invention means an extract obtained by extracting the above-mentioned Moringa leaves, mace or phloem. For the purpose of the present invention, the above extract preferably contains the moringa leaves, (Methanol, ethanol, butanol, etc.) or a mixed solvent thereof, more preferably a solvent such as ethyl acetate or a fermented alcohol, most preferably a fermented alcohol as a solvent But the present invention is not limited thereto. The resultant product includes all of the extract, the diluted or concentrated liquid of the extract, the dried product obtained by drying the extract, or the adjusted product or the purified product.

The term "fraction " of the present invention means a product obtained by a fractionation method for separating a specific component or a specific group from a mixture containing various constituents. In the present invention, preferably, the extract of Moringa leaf, mesophyll or copepod may be obtained by fractionation by various methods such as solvent fractionation method, ultrafiltration fractionation method, and chromatographic fractionation method.

The term "beta-glucan " of the present invention means that the glucose unit has a basic structure of? -Glycoside bond at positions 1 and 3, and the structure and physicochemical properties are changed It means a kind of polysaccharide. The beta-glucan does not directly attack cancer cells but activates the immune function of normal human cells by a nonspecific immune response to inhibit the proliferation and recurrence of cancer cells, activates macrophages, enters the body of cancer cells, By stimulating the secretion of cytokine, it activates immune function of immune cells T and B cells, exerts excellent blood glucose lowering effect and blood cholesterol reduction effect, and improves lipid metabolism to suppress body fat accumulation and accumulation, ≪ / RTI >

In the food composition of the present invention, since the beta-glucan is not present in a purified state in nature, the beta-glucan is separated and purified from a natural product containing beta-glucan or the food composition . The natural product including the beta-glucan is not particularly limited, but may be natural products such as mushroom, cereal, and yeast, and more preferably, natural mushroom, fenugus mushroom, gingko mushroom, shiitake mushroom, Can be a variety of mushrooms.

The content of Moringa leaf, seeds, beta-glucan and menthol contained in the food composition is not particularly limited, but is preferably 1 to 20% by weight of Moringa leaves, 1 to 10% by weight, By weight of beta-glucan and 1 to 10% by weight of beta-glucan.

The antioxidant food composition provided by the present invention can be used to prevent, treat or ameliorate various diseases caused by excessive active oxygen, or by the active oxygen, or consequently, excessive active oxygen. The diseases associated with active oxygen include, but are not limited to, degenerative neurological diseases including myocardial infarction, angina pectoris, coronary artery disease, ischemic heart disease, arteriosclerosis, Lou Gehrig's disease, Parkinson's disease, Alzheimer's disease, proximal axillary sclerosis and Huntington's disease May include various diseases such as cardiovascular diseases including ischemic brain diseases including stroke, diabetes, gastrointestinal diseases including gastritis and gastric cancer, aging, cancer, leukemia, aging, rheumatoid arthritis, hepatitis, atopic dermatitis, Preferably aging caused by active oxygen.

The food composition for antioxidation provided by the present invention can be prepared and taken in the form of food which can prevent or improve diseases caused by active oxygen. The content of the food composition contained in the food is not particularly limited, but may be in the range of 0.001 to 10% by weight, more preferably 0.1 to 1% by weight based on the total weight of the food. When the food is a beverage, it may be contained in a proportion of 1 to 10 g, preferably 2 to 7 g based on 100 ml. In addition, the food may contain additional ingredients which are commonly used as food compositions and which can improve odor, taste, visual appearance and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid and the like. In addition, it may include minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn) and copper (Cu) It may also include amino acids such as lysine, tryptophan, cysteine, valine, and the like. In addition, it is also possible to use antiseptics (such as potassium sorbate, sodium benzoate, salicylic acid, and sodium dehydroacetate), disinfectants (such as bleaching powder and highly bleached white powder, sodium hypochlorite), antioxidants (butylhydroxyanilide (BHA), butylhydroxytoluene BHT), etc.), coloring agents (such as tar pigments), coloring agents (such as sodium nitrite and sodium acetic acid), bleaching agents (sodium sulfite), seasoning (such as MSG sodium glutamate), sweeteners (such as hypoglycemia, , Food additives such as flavorings (vanillin, lactones, etc.), swelling agents (alum, potassium hydrogen D-tartrate), emulsifiers, emulsifiers, thickeners, encapsulating agents, gum bases, foam inhibitors, ) Can be added. The additives are selected according to the type of food and used in an appropriate amount.

On the other hand, the food composition can be used to produce a functional food for preventing or ameliorating diseases caused by active oxygen.

As a specific example, the food composition can be used to produce processed foods that can prevent or improve diseases caused by active oxygen. Such processed foods include, for example, confectionery, beverages, liquors, fermented foods, canned foods, processed milk products, processed foods, noodles, mushrooms and the like. The sweets include biscuits, pies, cakes, breads, candies, jellies, gums, cereals (including dinner utensils such as cereal flakes). Drinks include drinking water, carbonated beverages, functional ionic beverages, juices (such as apples, pears, grapes, aloes, citrus fruits, peaches, carrots, tomato juices, etc.) and sikhye. The mainstream includes sake, whiskey, shochu, beer, liquor, and fruit wine. Fermented foods include soy sauce, miso, and kochujang. Canned products include canned goods (eg, tuna, mackerel, saury, canned fish, etc.), canned products (beef, pork, chicken, turkey canned food, etc.) and canned products (corn, peach and canned pineapple). Milk processed foods include cheese, butter, yogurt and the like. Meat processed foods are pork cutlet, pork cutlet, chicken cutlet, sausage. Sweet and sour pork, nuggets, and nubani. And noodles such as sealed packaging raw noodles. In addition, the composition can be used for the production of various jelly products such as alginate mushroom, blue mushroom mushroom, acorn mushroom, omucum mushroom, mushroom mushroom, mushroom mushroom,

The term "functional food " of the present invention is the same term as " food for special health use (FoSHU) ", and includes medical and medical effects The food may be prepared in various forms such as tablets, capsules, powders, granules, liquids, rings and the like in order to obtain a useful effect for preventing or improving diseases caused by active oxygen.

As another embodiment for achieving the above object, the present invention provides a functional cosmetic exhibiting an antioxidative activity including Moringa leaf, moth, beta-glucan and menthol.

The term "cosmedical, cosmeceutical" of the present invention refers to a product having a professional function that has a physiologically active effect and an effect emphasized, unlike general cosmetics, A product that helps, a product that helps to improve wrinkles, a product that helps protect skin from burns or UV rays.

In the present invention, the functional cosmetic product refers to a product that exhibits antioxidative activity in various functional cosmetics to help prevent skin aging. The functional cosmetic product preferably includes, but is not limited to, a meringue leaf, a marigold, a beta-glucan, It can be a functional cosmetic for preventing aging of the skin as an active ingredient.

The functional cosmetic for preventing skin aging according to the present invention may further contain a cosmetic composition containing the above-mentioned Moringa leaf, mature, beta-glucan and menthol as an active ingredient and a commonly used cosmetic composition. For example, glycerol , Propylene glycol, 1,3-butylene glycol, sorbitol, polyethylene glycol, carboxyvinyl polymer, xanthan gum, carboxymethylcellulose, hydroxyethylcellulose, hydroxymethylcellulose, locust bean gum, allantoin, carrageenan and the like ; Paraffin wax, stearyl alcohol, carnauba wax, candelilla wax and calcium stearate, aluminum stearate, zinc stearate, and witchhazel can be used as the viscosity and hardness adjusting agent; As the ultraviolet absorber, butylmethoxydibenzoylmethane, octylmethoxycinnamate and the like can be used; Examples of the pigment include extender pigments such as titanium dioxide, fine particulate dioxides, kaolin, nylon powder, talc, sericite, mica and polymethyl methacrylate, and pigments such as yellow iron oxide, black iron oxide, red iron oxide, ultramarine, chromium oxide, Of a coloring pigment; Natural moisturizing agents such as 1,3-butylene glycol, concentrated glycerin, ethylene glycol, and chitin, chitosan, hypalonic acid, hyaluronic acid, lactic acid and glycolic acid can be used as the moisturizing agent; As the preservative, p-hydroxybenzoic acid esters, imidazolidinyl urea, and the like can be used. In addition, one or more of the above-mentioned components may be blended according to the characteristics of the product.

The functional cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

As another embodiment for achieving the above-mentioned object, the present invention provides a feed or feed additive exhibiting antioxidative activity including Moringa leaf, seedlings, beta-glucan and menthol.

The feed additive of the present invention can be used in a form in which a composition comprising a meringue leaf, a moth, a beta-glucan and a menthol is separately prepared in the form of a feed additive and mixed with the feed, or the meringue leaf, Can be used in a manner such that the composition is directly added to the feed composition.

The kind of the feed provided in the present invention is not particularly limited, and feeds conventionally used in the art can be used. Non-limiting examples of such feeds include vegetable feeds such as cereals, muscle roots, food processing busines logistics, algae, fibers, pharmaceutical buses, oils, fats, pastes or grain by-products; Animal feeds such as proteins, inorganic substances, fats, oils, fats, oils, monocellular proteins, animal plankton or foods. These may be used alone or in combination of two or more.

The feed additive of the present invention may be in a liquid or dry state, preferably in the form of a dried powder. The drying method for preparing the feed additive of the present invention in the form of a dried powder is not particularly limited and a method commonly used in the art can be used. Non-limiting examples of the drying method include air drying, natural drying, spray drying, and freeze drying. These may be used alone or in a manner that uses two or more methods together.

The feed additive of the present invention may further include other additives as required. Non-limiting examples of the above-mentioned usable additives include binders, emulsifiers, preservatives and the like which are added to prevent deterioration of feed or drinking water quality; A non-protein nitrogenous compound, a silicate, a buffer, a coloring agent, an extracting agent or an oligosaccharide to be added for the purpose of increasing the efficiency of feed or drinking water, In addition, a feed mixture or the like may be further included. These may be used alone or two or more of them may be added together.

As another embodiment for achieving the above object, the present invention provides a pharmaceutical composition for preventing or treating diseases associated with active oxygen, including Moringa leaves, mosquito bites, beta-glucan and somnolence.

As described above, since the moringa leaves, moths, beta-glucans and mites provided by the present invention exhibit antioxidative activity, the pharmaceutical composition containing them as an active ingredient can prevent, ameliorate, and treat diseases caused by active oxygen For example. At this time, diseases related to the active oxygen are as described above.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients or diluents conventionally used in the manufacture of pharmaceutical compositions. Specifically, the pharmaceutical composition may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to a conventional method . In the present invention, the carrier, excipient and diluent which may be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate , Calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, Sucrose, lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use may include various excipients such as wetting agents, sweetening agents, fragrances, preservatives, etc. in addition to water and liquid paraffin, which are simple diluents commonly used in suspension, liquid solutions, emulsions and syrups have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The content of the above-mentioned Moringa leaf, mesophyll, beta-glucan and menthol contained in the pharmaceutical composition of the present invention is not particularly limited, but is preferably 0.0001 to 50% by weight, more preferably 0.01 to 20% %. ≪ / RTI >

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount " of the present invention means a therapeutic or prophylactic treatment of a disease at a reasonable benefit / risk ratio applicable to medical treatment or prevention And the effective dose level refers to the level of the disease to be treated, the severity of the disease, the activity of the drug, the age, body weight, health, sex, sensitivity of the patient to the drug, Duration, duration of administration, factors involved in combination with or contemporaneously with the composition of the present invention, and other factors well known in the medical arts. The pharmaceutical composition of the present invention can be administered alone or in combination with known anti-cancer agents. It is important to take into account all of the above factors and administer an amount that will achieve the maximum effect in the least amount without side effects.

The dosage of the pharmaceutical composition of the present invention can be determined by those skilled in the art in consideration of the purpose of use, the degree of addiction to the disease, the age, body weight, sex, history, or kind of the substance used as the active ingredient. For example, the pharmaceutical composition of the present invention may be administered at about 0.1 ng to about 100 mg / kg, preferably 1 ng to about 10 mg / kg, per adult, and the frequency of administration of the composition of the present invention is particularly It is not limited, but it can be administered once a day or divided into several doses. The dose is not intended to limit the scope of the invention in any way.

In another aspect of the present invention, the present invention provides a pharmaceutical composition comprising, as active oxygen, a pharmaceutically effective amount of the above-mentioned pharmaceutical composition, which comprises the step of administering to a subject other than a human suffering from a disease caused by active oxygen Thereby providing a method for treating the disease.

The term "individual" of the present invention is intended to encompass a mammal including, but not limited to, rats, livestock, and the like, .

In the method for treating diseases caused by active oxygen of the present invention, diseases caused by active oxygen to be treated are the same as those described above.

In the method for treating diseases caused by active oxygen of the present invention, the route of administration of the pharmaceutical composition may be administered through any conventional route as long as it can reach the target tissue. The pharmaceutical composition of the present invention is not particularly limited, but may be administered by intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, intranasal administration, intrapulmonary administration, rectal administration and the like ≪ / RTI > However, oral formulations should be formulated so as to be coated with an active agent or protected from degradation from the top, since the moringa leaves, seeds, beta-glucan and gonads can be denatured by gastric acid upon oral administration. In addition, the composition may be administered by any device capable of transferring the active agent to the target cell.
According to another aspect of the present invention, there is provided a method of preparing a suspension comprising: 1) adding water to a mixed powder of a Moringa leaf powder and a beta-glucan powder to obtain a suspension; 2) adding sodium alginate to the suspension obtained in the step 1) to obtain a mixed suspension; 3) To the mixed suspension obtained in the step 2) is added 1 to 3% (w / w) of sodium phosphate, 1 to 3% (w / w) of calcium chloride and 1 to 3% (w / Adding and agitating the constituent solidifying solution; 4) solidifying the mixed suspension stirred in step 3) for 6 to 24 hours.

The composition provided by the present invention can be widely used for the development of a variety of functional foods having excellent physiological activity and texture because it exhibits excellent antioxidative activity and low calorie content and can be used for the production of functional food having excellent texture will be.

Fig. 1 is a photograph showing the results of TLC analysis of fractions showing antioxidative activity contained in Moringa leaf extract. Fig. 1 (A) shows the major components detected by applying 254 nm ultraviolet rays to the TLC substrate on which the active fractions were developed (B) is a photograph showing the result of detecting 8% copper sulfate and 1% phosphoric acid solution on a TLC substrate on which the active fraction is developed, followed by carbonization at 200 ° C.
Fig. 2 is a graph and a graph showing the absorbance measured at 405 nm and the standard curve used for measuring the content of reducing sugar contained in the Moringa leaf extract. The absorbance was measured by performing independent experiments three times.
FIG. 3 is a graph showing the results of comparing the antioxidative activities of each of alginic acid jelly including mesophyllum, Lepidoptera, Moringa extract, Paella, Beta-glucan, Moringa leaves or combinations thereof.
FIG. 4 is a graph showing the activity of each alginate ferric reducing activity (FRAP, Ferric Reducing Antioxidant Power), including alfalfa, lepidopteran, Moringa leaf extract, parasitic b-glucan, In the graph of FIG.
5 is a graph showing the results of comparing antioxidative activities of alginate mushroom containing beta-glucan and / or Meringa leaves.

Hereinafter, the present invention will be described in more detail with reference to examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  One: Moringa  Analysis of Antioxidant Activity Components of Leaves

The main components of Moringa leaf were analyzed using TLC. Specifically, the powder of Moringa leaf was added to sterilized distilled water so as to be 1 wt%, and the suspension was allowed to stand at room temperature for 12 hours to obtain an extract of Moringa leaf. 10 μl of the extract of Moringa leaf obtained above was placed on a TLC substrate and developed by adding a developing solvent (isopropanol: ammonia water: distilled water = 70: 15: 15, v / v / v) (Wavelength: 254 nm), or the detection solution (1% copper sulfate and 8% phosphoric acid solution) for sugar detection was added and then carbonized at 200 ° C for analysis.

Fig. 1 is a photograph showing the results of TLC analysis of fractions showing antioxidative activity contained in Moringa leaf extract. Fig. 1 (A) shows the major components detected by applying 254 nm ultraviolet rays to the TLC substrate on which the active fractions were developed (B) is a photograph showing the result of detecting 8% copper sulfate and 1% phosphoric acid solution on a TLC substrate on which the active fraction is developed, followed by carbonization at 200 ° C. As shown in FIG. 1, the components exhibiting antioxidative activity contained in the Moringa leaves were analyzed as glycosides absorbing 254 nm UV or glucose-like monosaccharides having a molecular weight size of about 254 nm.

Example  2: Moringa  Reducing sugar contents of leaves

From the results of Example 1, it was analyzed that the component exhibiting antioxidative activity contained in the Moringa leaf was a saccharide or glycoside absorbing ultraviolet light of a specific wavelength. Therefore, the content of reducing sugar contained in the Moringa leaf was analyzed. Specifically, a 2-fold volume of 0.0025 g / ml PHAHAH solution (4-hydroxy-benzhydrazide in 0.5 M NaOH) was mixed with the Moringa leaf extract prepared in Example 1, followed by reaction at 97 ° C for 5 minutes, After cooling, the cooled reaction product was measured for absorbance at 405 nm and then applied to a standard curve to calculate the content of reducing sugar (FIG. 2).

Fig. 2 is a graph and a graph showing the absorbance measured at 405 nm and the standard curve used for measuring the content of reducing sugar contained in the Moringa leaf extract. The absorbance was measured by performing independent experiments three times. As shown in FIG. 2, the content of reducing sugar contained in the Moringa leaf extract was found to be about 0.2%.

Example  3: Activity analysis of alginate mushroom containing various natural products

Example  3-1: Preparation of alginate mushroom containing various natural products

First, 10 g of the sterilized liquor was pulverized with a mixer to obtain a milled product. 500 g of water was added to 10 g of the pulverized product to obtain a suspension, and 20 g of sodium alginate was added to the suspension to obtain a mixed suspension. The same volume of solidification solution (2% sodium phosphate, 2% calcium chloride, and 2% anhydrous alum) was added to the above suspension, stirred slowly, and then poured into a mold and solidified for 12 hours to prepare alum gum containing mash.

Next, each alginate powder was prepared in the same manner as described above, except that alfalfa wormwood, Moringa extract, parasitic, beta-glucan, moringa leaves or a combination thereof was used in place of the mascara. Respectively.

Example  3-2: Antioxidant activity analysis

The free radical scavenging activity of each of the alginic acid prepared in Example 3-1 was analyzed by the DPPH method. Specifically, 0.9 ml of a 0.2 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) solution was added to 10 mg of each of alginic acid jelly prepared in Example 3-1, stirred vigorously, Min. ≪ / RTI > After the reaction was completed, the absorbance at 517 nm was measured, and the measured absorbance was used to calculate the free radical scavenging activity (FIG. 3).

FIG. 3 is a graph showing the results of comparing the antioxidative activities of each of alginic acid jelly including mesophyllum, Lepidoptera, Moringa extract, Paella, Beta-glucan, Moringa leaves or combinations thereof. As shown in FIG. 3, it was confirmed that various alginate mums including Moringa leaves exhibit antioxidant activity. However, it can be interpreted that the antioxidative activity of jelly containing meringa leaf alone was measured only by the result of Fig. 3. However, when compared with the jelly containing ingredients other than the meringue leaf, Since the content is high, it can not be said that the moxa-containing moxa alone has the best antioxidative activity.

Example  3-3: Metal reduction activity ( FRAP , Ferric Reducing Antioxidant Power )analysis

Each alginate powder prepared in Example 3-1 was analyzed for the level of metal reduction activity, which is a kind of antioxidant activity. The metal reduction activity is generally determined by the amount of polyphenol- It is used to measure the dose of antioxidants. Specifically, 1.5 mL of FRAP reagent containing 300 mM sodium acetate buffer (pH 3.6), 10 mM TPTZ solution and 20 mM FeCl ₃ solution in a ratio of 10: 1: 1 (v / v / v) , And the reaction was carried out at 37 ° C for 15 minutes. Then, the absorbance of the reaction supernatant was measured at 593 nm, and the metal reduction activity was calculated from the result (FIG. 4).

FIG. 4 is a graph showing the activity of each alginate ferric reducing activity (FRAP, Ferric Reducing Antioxidant Power), including alfalfa, lepidopteran, Moringa leaf extract, parasitic b-glucan, In the graph of FIG. As shown in FIG. 4, it was confirmed that the alginate paste containing moringa leaves showed the most excellent metal reduction activity.

Example  3-4: Beta- Glucan  And / or Moringa  Antioxidant activity analysis of alginate mushroom containing leaves

First, 10 g of a sterilized meringue leaf was pulverized with a mixer to obtain a pulverized Meringa leaf. A suspension was obtained by adding 500 ml of water to 10 g of the above-obtained Moringa leaf flesh and / or beta-glucan, and 20 g of sodium alginate was added to the suspension to obtain a mixed suspension. The same volume of solidifying solution (2% sodium phosphate, 2% calcium chloride and 2% anhydrous alum) was added to the mixed suspension and stirred slowly, then poured into a mold and solidified for 12 hours to give beta glucan, moringa leaves or betaglucan / Alginic acid jelly containing Moringa leaves was prepared.

Next, the free radical scavenging activity was calculated using the method of Example 3-2, except that each alginate powder prepared above was used, and converted into a ratio relative to the antioxidative activity of the control group ( 5). At this time, ascorbic acid (AA (s)) was used as a control group.

5 is a graph showing the results of comparing antioxidative activities of alginate mushroom containing beta-glucan and / or Meringa leaves. As shown in FIG. 5, alginate mushroom containing all of these than beta-glucan or moringa mushroom individually showed an excellent antioxidative activity, and the antioxidant activity showed antioxidative activity of ascorbic acid Which is about 65%.

Claims (13)

1) adding water to a mixed powder of a moringa leaf powder and a beta-glucan powder to obtain a suspension;
2) adding sodium alginate to the suspension obtained in the step 1) to obtain a mixed suspension;
3) To the mixed suspension obtained in the step 2) is added 1 to 3% (w / w) of sodium phosphate, 1 to 3% (w / w) of calcium chloride and 1 to 3% (w / Adding and agitating the constituent solidifying solution;
4) solidifying the stirred suspension in step 3) for 6 to 24 hours;
≪ / RTI > characterized in that it comprises a moringa leaf.
The method according to claim 1,
Wherein the mixed powder of step 1) further comprises a mashing powder and a lemon balm wormwood powder.
The method according to claim 1,
Wherein the content of the Moringa leaves of step 1) is contained in an amount of 0.01 to 20% by weight based on the total weight of the meringue leaves.
delete delete A mushroom containing a meringue leaf having excellent antioxidant activity produced by the method according to any one of claims 1 to 3.
delete delete delete delete delete delete delete

Images