KR102331119B1 - Composition for preventing skin anti-aging comprising fermented black rice extract and fermented peanut sprout extract - Google Patents

Abstract

The present invention relates to a composition for preventing skin aging comprising a fermented product of black rice extract and a fermented product of peanut sprout extract, wherein the fermented product of black rice extract and fermented product of peanut sprout extract of the present invention acts as an antioxidant to prevent skin aging Because it shows the effect, it can be used as a material for cosmetics and food for skin aging prevention.

Description

A composition for preventing skin aging comprising a fermented product of black rice extract and a fermented product of peanut sprout extract

The present invention relates to a composition for preventing skin aging comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

Free radicals generated during metabolic processes in the human body are molecules that cause damage to living tissues and abnormal activities of living organs during oxidation. Unlike oxygen, it loses one electron and is in an unstable state. Therefore, active oxygen is oxygen with strong oxidizing power and adversely affects living tissues and cells. Therefore, it is also known as harmful oxygen. Active oxygen in the body increases due to excessive oxygen generation due to environmental factors such as environmental pollution and ultraviolet rays, blood circulation disorders in the living body, and stress. will cause It causes obesity, arteriosclerosis, diabetes and skin aging, and it is known that 90% of modern diseases are related to free radicals.

Environmental pollution and various stresses increase the concentration of reactive oxygen species in the body, and these reactive oxygen species attack lipids, proteins, sugars and DNA, which are cellular components, and cause a peroxidation reaction, thereby promoting skin aging. . Accordingly, there is a demand for development of a cosmetic composition having an anti-aging (anti-aging skin) effect by performing the antioxidant effect of inhibiting the generation of reactive oxygen species and the antioxidant action as described above.

Black rice contains α,β,γ,δ-tocopherol, tocotrienol, phytosterol, and various phenolic compounds. It has a higher nutrient content than general white rice, such as dietary fiber, minerals, unsaturated fatty acids, and dietary fiber. In comparison, it is rich in flavonoids and anthocyanins.

Peanut is an annual herbaceous plant belonging to the legume family. It is a high-calorie food containing a lot of fat and protein. Peanut is known as a representative plant containing resveratrol, a natural polyphenol compound, and is known to be effective in anti-diabetes, anti-aging, heart disease prevention, diet, etc. as an anti-cancer and antioxidant active substance, and also protects cells from free radicals It has been reported to have many beneficial health effects, such as prevention of diabetes, obesity, and cancer, as it has an excellent antioxidant action.

Among vegetables, sprout vegetables and young leaves refer to young sprouts from which seeds have been germinated. Although it is smaller in size than mature vegetables, it has been reported to have excellent nutritional aspects (Park SG, Korean J Fac Hort Res, 1989, 2: 34 ; Khalil er al., LWT, 2007, 40: 937-945). When sprouts germinate, it produces physiologically active substances to protect itself from external enemies such as mold, bacteria, and UV.

Recently, many studies have been conducted on complexes that increase physiological activity through processes such as fermentation or mixing with other samples by utilizing the antioxidant performance of a single sample, rather than just checking the antioxidant performance. Fermentation, one of the most commonly used technologies, is a process in which lactic acid bacteria produce lactic acid as the main product in an anaerobic environment. Lactobacillus is known to exhibit various effects such as immunity enhancement, elimination of harmful bacteria in the intestine, anticancer effect, prevention of constipation, and improvement of liver cirrhosis. Even now, research on fermented samples is being actively conducted, and it is in the spotlight as a health functional food.

Accordingly, the present inventors confirmed the antioxidant activity and skin aging prevention effect of black rice extract and peanut sprout extract fermented with lactic acid bacteria, and completed the present invention.

Korean Patent No. 10-1825803

It is an object of the present invention to provide a cosmetic composition for preventing or improving skin aging comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

Another object of the present invention is to provide a health functional food composition for preventing or improving skin aging, comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

Another object of the present invention is to provide a health food composition for preventing or improving skin inflammation comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

In order to achieve the above object,

The present invention provides a cosmetic composition for preventing or improving skin aging, comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

In addition, the present invention provides a health functional food composition for preventing or improving skin aging comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

Furthermore, the present invention provides a health food composition for preventing or improving skin inflammation comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

Since the fermented product of the black rice extract and the fermented product of the peanut sprout extract of the present invention exhibits an anti-aging effect on the skin by acting as an antioxidant, it can be used as a material for cosmetics and food for preventing skin aging.

1 shows the measurement result of the DPPH radical scavenging activity of the composition according to the present invention.
2 shows the measurement results of the ABTS radical scavenging activity of the composition according to the present invention.
3 shows the measurement results of the nitrite scavenging activity of the composition according to the present invention.
Figure 4 shows the measurement result of the reducing power of the composition according to the present invention.

Hereinafter, the present invention will be described in detail.

Cosmetic composition for preventing or improving skin aging

The present invention provides a cosmetic composition for preventing or improving skin aging, comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

As used herein, the term "extract" refers to a preparation concentrated by squeezing an extraction target with an appropriate leachate and evaporating the leachate. , may be a dried product obtained by drying the extract, a crude product or a purified product thereof. The fermented product of the black rice extract and the fermented product of the peanut sprout extract may be prepared using a general extraction method, separation and purification method known in the art. The extraction method is not limited thereto, but preferably methods such as hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction may be used.

In the composition of the present invention, the extract may be extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably extracted with 70% ethanol, but is not limited thereto.

In the composition of the present invention, the fermented product is a fermented product obtained by fermenting black rice extract or peanut sprout extract with lactic acid bacteria ( Lactobacillus plantarum ), and the fermentation may be fermented at 30 to 40° C. for 48 to 96 hours, preferably It can be fermented for 70 to 74 hours at 35 to 40 °C.

In one embodiment of the present invention, the fermented product of the black rice extract and the fermented product of the peanut sprout extract is immersed and extracted by adding 30 to 70 times ethanol to the black rice powder (step 1); immersion extraction by adding 10 to 20 times ethanol to the peanut sprout powder (step 2); and mixing the black rice ethanol extract of step 1 and the peanut sprout ethanol extract of step 2 in a volume ratio of 1: 0.3 to 1, and inoculating and fermenting lactic acid bacteria (Lactobacillus plantarum) by 1 to 3% of the volume of the mixture. (Step 3); can be prepared including.

The cosmetic composition of the present invention may be prepared including a cosmetically effective amount of the active ingredient of the present invention described above and a cosmetically acceptable carrier.

The appearance of the cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. It is in any dosage form suitable for topical application, for example solutions, gels, solids, kneaded dry products, emulsions obtained by dispersing the oily phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a non-ionic vesicular dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or concealer stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its formulation, for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream. , nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

According to an embodiment of the present invention, the content of the active ingredient is not particularly limited, but the extract according to the present invention may be included in an amount of 0.001 to 20% by weight based on the total weight of the composition. When the above content is satisfied, excellent efficacy can be exhibited without side effects.

The cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the fermented product of the black rice extract and the fermented product of the peanut sprout extract. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

In the cosmetic composition of the present invention, a component contained in a general cosmetic composition may be further blended with the functional additive as needed. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation An accelerator, a cooling agent, a limiting agent, purified water, etc. are mentioned.

Health functional food or health food composition for preventing or improving skin aging

The present invention provides a health functional food or health food composition for preventing or improving skin aging, comprising a fermented product of black rice extract and a fermented product of peanut sprout extract.

In the composition of the present invention, the extract may be extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably extracted with 70% ethanol, but is not limited thereto.

In the composition of the present invention, the fermented product is a fermented product obtained by fermenting black rice extract or peanut sprout extract with lactic acid bacteria ( Lactobacillus plantarum ), and the fermentation may be fermented at 30 to 40° C. for 48 to 96 hours, preferably It can be fermented for 70 to 74 hours at 35 to 40 °C.

When the fermented product of the black rice extract and the fermented product of the peanut sprout extract of the present invention is used as a health functional food and health food composition, there is no particular limitation on the type of food. Examples of health functional foods to which the fermented product of the black rice extract of the present invention and the fermented product of the peanut sprout extract can be added may be in the form of tablets, capsules, pills or liquids, and examples of health foods include various drinks, meat, sausages. , bread, candy, snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gum, tea, and vitamin complexes, and includes all health functional foods and health food compositions in a conventional sense.

The health functional food and health food composition containing the fermented product of the black rice extract and the fermented product of the peanut sprout extract according to the present invention may be added to food as it is or used together with other foods or food ingredients, and may be appropriately prepared according to a conventional method. can be used The mixing amount of the fermented product of the black rice extract and the fermented product of the peanut sprout extract may be suitably determined according to the purpose of its use (for prevention or improvement). In general, the amount of the composition in the health functional food and health food composition may be added in an amount of 0.1 to 90 parts by weight based on the total weight of the food. However, in the case of long-term intake for health maintenance or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the fermented product of black rice extract and the fermented product of peanut sprout extract are It can also be used in amounts beyond the range.

The health functional food and health food composition of the present invention is not particularly limited in other ingredients except for containing the fermented product of the black rice extract of the present invention and the fermented product of the peanut sprout extract as essential components in the indicated ratio, and various Flavoring agents or natural carbohydrates may be contained as additional ingredients. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatine, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 health functional food and health food composition of the present invention.

In addition to the above, the health functional food and health food composition containing the fermented product of the black rice extract and the fermented product of the peanut sprout extract of the present invention is a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors , colorants and thickeners (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, etc. may contain. In addition, the health functional food and health food composition of the present invention may contain natural fruit juice, fruit juice, and fruit for the production of fruit juice drinks and vegetable drinks.

These components may be used independently or in combination. Although the ratio of these additives is not so important, it is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the health functional food and health food composition containing the fermented product of the black rice extract and the fermented product of the peanut sprout extract of the present invention. am.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following examples.

<Example 1> lactic acid bacteria ( Lactobacillus plantarum ) fermented black rice extract

After finely powdering black rice, it was put into 50 liters of 70% ethanol per 1 kg of black rice powder at room temperature and immersed and extracted three times. After filtration of the extract with filter paper, the filtrate was concentrated at 40° C. and then freeze-dried to prepare a black rice extract.

After dissolving the produced black rice extract in 1L of distilled water per 100g, lactic acid bacteria ( Lactobacillus plantarum) was inoculated as much as 2% of the volume. After inoculation, it was cultured at 37° C. for 3 days to prepare a fermented black rice extract, abbreviated as BRF. After the freeze-drying process, it was ground into a powder and used in the experiment.

<Example 2> lactic acid bacteria ( Lactobacillus plantarum ) fermented black rice extract and peanut sprout extract

<2-1> Preparation of black rice extract

After finely powdering black rice, it was immersed and extracted three times at room temperature in 50 liters of 70% ethanol per 1 kg of black rice powder. After filtration of the extract with filter paper, the filtrate was concentrated at 40° C. and then freeze-dried to prepare a black rice extract.

<2-2> Preparation of peanut sprout extract

For the peanut sprout extract, peanut sprouts 10 days after germination were used. Peanut sprouts were dried in a hot air dryer at 50° C. for 48 hours. After the dried peanut sprouts were crushed finely, they were immersed and extracted three times in 10 times 70% ethanol. After filtering the extract with filter paper, the filtrate was concentrated at 40° C. and then freeze-dried to make a peanut sprout extract.

<1-3> Lactobacillus ( Lactobacillus plantarum ) to ferment

The black rice extract of Example 2-1 and the peanut sprout extract of Example 2-2 were mixed in a volume ratio of 2:1, and the extract mixture was dissolved in 1 L of distilled water per 100 g, and then lactic acid bacteria ( Lactobacillus plantarum) was added to the volume of 2% inoculation. After inoculation, cultured at 37° C. for 3 days to prepare fermented black rice extract and peanut sprout extract, abbreviated as BRPSF. After the freeze-drying process, it was ground into a powder and used in the experiment.

<Comparative Example 1> lactic acid bacteria ( Lactobacillus plantarum ) fermented peanut sprout extract

For the peanut sprout extract, peanut sprouts 10 days after germination were used. Peanut sprouts were dried in a hot air dryer at 50° C. for 48 hours. After the dried peanut sprouts were crushed finely, they were immersed and extracted three times in 10 times 70% ethanol. After filtering the extract with filter paper, the filtrate was concentrated at 40° C. and then freeze-dried to make a peanut sprout extract.

Then, after dissolving the peanut sprout extract in 1L of distilled water per 100g, lactic acid bacteria ( Lactobacillus plantarum) was inoculated as much as 2% of the volume. After inoculation, cultured at 37° C. for 3 days to prepare a fermented peanut sprout extract, abbreviated as PSF. After the freeze-drying process, it was ground into a powder and used in the experiment.

<Experimental Example 1> Measurement of DPPH radical scavenging activity

The DPPH radical scavenging activity (Electron donating abilities, EDA) of the extract was measured as follows. Comparative Example 1 (PSF), Example 1 (BRF) and Example 2 (BRPSF) were prepared at a concentration of 100 μg/ml, and 80 μl of a 0.2 mM DPPH solution was dispensed into 80 μl of each sample solution. After that, completely wrapped with foil and allowed to react at room temperature for 30 minutes. After the reaction, absorbance was measured at 517 nm (ELISA reader (16039400, TECAN). The antioxidant power of the extract was expressed as IC ₅₀ (half maximal inhibitory concentration) indicating the concentration showing the intermediate activity of radical scavenging activity. That is, DPPH radical It refers to the concentration at a value that reduces the absorbance by 50%.

As a result of measuring the DPPH radical scavenging activity, as shown in FIG. 1 , the DPPH radical IC ₅₀ of Comparative Example 1 (PSF), Example 1 (BRF) and Example 2 (BRPSF) was measured. The peanut sprout extract was 11.7 mg/ml, the fermented black rice extract of Example 1 was 9.6 mg/ml, and the fermented black rice extract and the peanut sprout extract of Example 2 were 4.5 mg/ml, respectively, more mixed than when alone. In the case of use, it was confirmed that the antioxidant activity was more excellent.

<Experimental Example 2> ABTS ⁺ Measurement of radical scavenging activity

For ABTS ⁺ radical scavenging activity experiment, ABTS ⁺ radical cation is a solution of 72,2'-azinbis-3-ethyl-benzothiazoline-6-sulfonic acid and distilled water mixed with 2.45mM potassium persulfate in a 1:1 ratio with foil. Wrapped and reacted at room temperature for 12 to 16 hours. The reacted solution was mixed with 0.01M PBS (pH 7.4) so that the absorbance value at 734nm was 0.70±0.02. _{The antioxidant power of the extract was expressed as IC 50} (half maximal inhibitory concentration) indicating the concentration showing the intermediate activity of radical scavenging activity. That is, it refers to a concentration of a value that reduces the absorbance of the ABTS radical by 50%.

As a result of measurement of ABTS ⁺ _{radical scavenging activity, as shown in FIG. 2 , the DPPH radical IC 50} of Comparative Example 1 (PSF), Example 1 (BRF) and Example 2 (BRPSF) was measured. The peanut sprout extract was 14.6 mg/ml, the fermented black rice extract of Example 1 was 13.2 mg/ml, and the fermented black rice extract and the peanut sprout extract of Example 2 were 7.8 mg/ml, respectively, more mixed than when alone In the case of use, it was confirmed that the antioxidant activity was more excellent.

<Experimental Example 3> Measurement of nitrite scavenging activity

For the nitrite scavenging ability, Gray and Dugan's experimental method was used. Comparative Example 1 (PSF), Example 1 (BRF) and Example 2 (BRPSF) were measured at concentrations of 0, 100, 200, 300, 400, and 500 μg/ml, respectively. 120 μl of each concentration was dispensed into an E-tube, _{mixed with 120 μl of NaNO 2} and 960 μl of 0.2M citrate buffer (pH 3.0), and reacted in a water bath at 37° C. for 1 hour. 200 μl of the solution reacted in the E-tube, 400 μl of acetic acid, and 40 μl of each of Griess reagents A and B were dispensed and reacted at room temperature for 15 minutes. After dispensing in a 96 well plate, absorbance was measured at 520 nm using an ELISA reader (16039400, TECAN).

Scavenging activity (%) = {1-(Absorbance of treated sample - Absorbance of sample or standard)/ Absorbance of control} ×100

As a result of measuring the nitrite scavenging activity, as shown in FIG. 3 , it was confirmed that the nitrite scavenging activity increased in a concentration-dependent manner, and the fermentation of Example 2 compared to the fermented peanut sprout extract of Comparative Example 1 or the fermented black rice extract of Example 1 In the case of the mixed use of black rice extract and peanut sprout extract, it was confirmed that the antioxidant activity was better.

<Experimental Example 4> Measurement of reducing power

The reducing power measurement was carried out with slight modifications according to the method described by Chung et al. (Chung et al. 2005). Gray and Dugan's experimental method was used. Standard ascorbic acid and samples were measured at various concentrations. 120 μL of each concentration was dispensed into an E-tube, _{mixed with 120 μL of NaNO 2 and} 960 μL of 0.2M citrate buffer (pH 3.0), and reacted in a water bath at 37° C. for 1 hour. After dispensing 200 μL of the solution reacted in the E-tube, 400 μL of acetic acid, and 40 μL of each of Griess reagents A and B, the mixture was reacted at room temperature for 15 minutes. After dispensing in a 96 well plate, absorbance was measured at 520 nm using an ELISA reader (16039400, TECAN).

As a result of measuring the reducing power, as shown in FIG. 4 , it was confirmed that the reducing power increased in a concentration-dependent manner, and the fermented black rice extract of Example 2 and the fermented black rice extract of Example 2 rather than the fermented peanut sprout extract of Comparative Example 1 or the fermented black rice extract of Example 1 In the case of the mixed use of peanut sprout extract, it was confirmed that the reducing power was better.

<Experimental Example 5> Measurement of MMP-1 inhibitory activity

As the skin ages due to various internal and external factors, the keratinocytes change irregularly, the fibroblasts and inflammatory cells increase, the blood vessel wall becomes thinner, and the decrease of collagen fibers mainly present in the dermis is accelerated (Physiology of Skin aging. Clin Plast Surg. 2012. 39:1-8.), the decrease in collagen is mainly secreted by fibroblasts in the dermis and is caused by matrix metalloproteinases (MMPs), a group of enzymes that degrade the extracellular matrix. It is known that it is mainly caused by (The role of TRPV1 channel in aged human skin. J Dermatol Sci. 2012. 65:81-85.).

By measuring the concentration after UV irradiation and sample addition of MMP-1 associated with aging, the effect of inhibiting MMP-1 expression by the sample was confirmed. For this purpose, an enzyme immunoassay (ELISA) was performed as follows.

Using a UV chamber, UVA was irradiated to human dermal fibroblasts with an energy of ^{5 J/cm 2 .} In order to determine the amount of UV irradiation and incubation time, a condition in which the MMP expression level in fibroblasts is maximized was confirmed through a preliminary experiment. The negative control group was wrapped in silver foil and maintained in the UVA environment for the same time. UVA emission was measured using a UV radiometer. Cells during UVA irradiation were as previously dispensed, and after UVA irradiation, the sample was exchanged with the medium, and the culture medium was recovered after culturing for 24 hours and coated on 96-wells.

MMP-1 (Ab-5) monoclonal antibody and MMP-2 (Ab-3) monoclonal antibody were treated as primary antibodies, and reacted at 37°C for 60 minutes. Secondary antibody, anti-mouse IgG (whole mouse, alkaline phosphatase conjugated) was reacted again for 60 minutes, and then an alkaline phosphatase substrate solution (1 mg/ml ρ-nitrophenyl phosphate in diethanolamine buffer) was reacted at room temperature for 30 minutes. Then, the absorbance was measured at 405 nm using a microplate reader (UVT-06685, Termo max, USA). In this case, as a negative control, no sample was used, and as a positive control, retinol, which is known to have excellent inhibitory effect on MMP-1 expression, was used. The MMP-1 expression inhibition rate (%) based on the sample treatment concentration of 001% is shown in Table 1 below.

MMP-1 expression inhibition rate (%) Comparative Example 1 40.1 Example 1 49.9 Example 2 85.4 Positive control-retinol 42.7

As a result, as shown in Table 1, in the case of the mixed use of the fermented black rice extract and the peanut sprout extract of Example 2 of the present invention, compared to the retinol used as a positive control, it exhibited an excellent effect more than twice. Due to the above results, it was confirmed that in the case of the fermented product of the black rice extract and the fermented product of the peanut sprout extract of the present invention, it was possible to inhibit the MMP enzyme expression, thereby preventing collagen degradation, thereby inhibiting the decrease in skin elasticity and the formation of wrinkles. .

<Statistics processing>

All data are presented as mean±standard deviation (SDM). All experiments were repeated three times. For comparison of measured values between each group, one-way analysis of variance (ANOVA) test was performed and the difference was verified at the significance level of p < 0.05. GraphPad Prism 5, Sigma plot 10.0 and Microsoft Excel 2007 were used for graph evaluation and statistics.

So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated not in the foregoing description, but particularly in the claims, and all differences within an equivalent scope should be construed as being included in the present invention.

Claims (11)

A cosmetic composition for preventing or improving skin aging comprising a fermented product obtained by fermenting a mixture of black rice extract and peanut sprout extract in a volume ratio of 1: 0.3 to 1 with Lactobacillus plantarum as an active ingredient. According to claim 1,
The extract is a cosmetic composition, characterized in that extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. delete According to claim 1,
The fermentation is a cosmetic composition, characterized in that the fermentation is 48 to 96 hours at 30 to 40 ℃. According to claim 1,
The cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, soap, cleansing A cosmetic composition, characterized in that at least one formulation selected from formulations consisting of foam, cleansing lotion, cleansing cream, body lotion and body cleanser. A health functional food composition for preventing or improving skin aging, comprising a fermented product obtained by fermenting a mixture of black rice extract and peanut sprout extract in a volume ratio of 1: 0.3 to 1 with Lactobacillus plantarum as an active ingredient. A health food composition for preventing or improving skin inflammation, comprising a fermented product obtained by fermenting a mixture of black rice extract and peanut sprout extract in a volume ratio of 1: 0.3 to 1 with Lactobacillus plantarum as an active ingredient. 8. The method of claim 6 or 7,
The extract is a composition, characterized in that extracted with water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. delete 7. The method of claim 6,
The health functional food is a health functional food composition, characterized in that the food in the form of tablets, capsules, pills or liquids. 8. The method of claim 7,
The health food is a health food composition, characterized in that it is selected from various drinks, meat, sausage, bread, candy, snacks, noodles, ice cream, dairy products, soup, ionic beverages, beverages, alcoholic beverages, gum, tea and vitamin complexes.

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