KR101863895B1 - Composition for skin whitening comprising Stachys sieboldii extract fermented using Hericium erinaceum mycelium as effective component - Google Patents

Abstract

The present invention relates to a skin-whitening composition containing a fermented Stachys japonica Miq. extract fermented by using Hericium erinaceus (Bull.) Pers. mycelium as an active ingredient. More specifically, the hot water extract of the fermented Stachys japonica Miq. fermented by using the Hericium erinaceus (Bull.) Pers. Mycelium has almost no cytotoxicity in comparison with hot water extracts of Stachys japonica Miq, and remarkably inhibits activities of tyrosinase, thereby being safe and useful for drugs and health functional foods for preventing, ameliorating, or treating hyperpigmentation by melanin or cosmetic products for skin whitening.

Description

TECHNICAL FIELD The present invention relates to a composition for skin whitening comprising a fermented product of a rootstock sleep extract fermented by using a mushroom mycelium of Aspergillus oryzae as an active ingredient (Composition for skin whitening comprising Stachys sieboldii extract fermented using Hericium erinaceum mycelium as effective component)

The present invention relates to a composition for whitening skin, which contains, as an active ingredient, a fermented product of a rootstock mire extract obtained by fermentation using a mushroom mycelium.

The skin is directly exposed to the external environment of the body tissues and serves as a barrier between the inside and outside environment of the human body to protect the living environment from the surrounding environment by protecting against the infiltration of foreign pollutants and microorganisms including chemicals and ultraviolet rays . Skin is also characterized by melanin, hemoglobin, carotene, etc. Melanin is the most important factor. In addition to determining human skin color, melanin also performs skin protection functions such as ultraviolet absorbing action, free radical scavenger and the like. However, excessive production of melanin due to external environmental changes such as overexposure of ultraviolet rays, air pollution, stress, and the like causes pigmentation in the skin, which causes skin melanism, spots and freckles. Blackening of the skin is caused by the reaction of the skin cells to internal and external factors, and a typical factor is due to ultraviolet exposure. That is, when the skin is exposed to ultraviolet rays, tyrosinase is activated. The tyrosinase acts on tyrosine present in the skin tissue and is oxidized to generate dopa (DOPA) and dopaquinone, A polymer called melanin is synthesized in the melanosome in the skin pigment cell melanocyte. The melanin is transferred to the keratinocyte which is the keratinocyte of the skin and reaches the skin surface by the keratinization process Protects skin from ultraviolet rays. Therefore, melanin is an essential UV inhibitor in the human body and also plays an effective role as an effective radical scavenger for eliminating various radicals that modify biological components such as proteins, lipids, and nucleic acids. However, when melanin is over-synthesized locally, or when the skin's physiological functions due to skin lesions and aging deteriorate, melanin deposits on the skin surface causing spots, freckles and various pigment deposits. As described above, the causes and mechanisms of skin blackening are revealed. In the production of whitening cosmetic materials, substances having an activity inhibiting effect of tyrosinase, which is an enzyme involved in the skin darkening process, are added to cosmetics, A method of reducing the production of melanin by inhibiting the reaction is generally used. Representative materials used for this purpose include chemical substances such as ascorbic acid, kojic acid, and hydroquinone, plant extracts such as extracts of Cabbage bark extract and licorice extract. However, since ascorbic acid is not only insufficient in inhibiting tyrosinase activity, but also has low stability of the molecule itself, it is not suitable as an inhibitor of melanogenesis, and kojic acid is excellent in inhibitory activity of tyrosinase. However, when ascorbic acid is blended in cosmetics, Therefore, there is a problem in stability such as decreased potency, and there is a limit in use due to a large skin irritation. Hydroquinone has been limited in its use in cosmetics due to skin irritation and safety problems. Therefore, attention is focused on the development of new and effective whitening ingredients.

Meanwhile, Stachys sieboldii miq.) is a perennial herbaceous plant belonging to the family Lamiaceae and grows in height of 30 ~ 60cm. The flower is magenta and runs from the middle of May to the end of August to the upper part of the branches and stems and blooms in the layer. The roots are bulbous parts that look like bulbs, and they are 1 ~ 3cm long and rhombic. The roots are red and milky. Other names are known as asterisks, asterisks, asterisks, zizam, tochoncho, supernaturalis, jiyu, earthen, tortoise, wawa. The efficacy is known to be effective for fever, seawater, pneumonia, toxin, hysteria, hemorrhage, swelling, inflammation, analgesia, gusts, appendicitis, sore throat, mild fever, swelling. Especially recently, phenylethanoid glycoside has been used for myasthenia, , It is known that it is effective for prevention of senile dementia and improvement of memory.

Hericium erinaceum ) is a mushroom belonging to the group of coral mushrooms. It is known as a hardwood or bark of broad-leaved trees in autumn. It is also known as monkey head mushroom and bear head mushroom in China. Yamabushitake in Japan and Yamabushitake It is also known as Mushroom Mushroom. It is known that the mushroom is rich in carbohydrates, proteins, amino acids, enzymes, inorganic salts and vitamins, and has an anticancer and immunological function. In recent years, it has been known to contain various physiologically active substances It is reported that

Korean Patent Laid-Open Publication No. 2016-0024525 discloses a cosmetic composition comprising as an active ingredient a base coat composition, and Korean Patent Publication No. 2004-0090114 discloses a composition for external application for skin containing an extract of Aspergillus oryzae . However, the composition for skin whitening, which contains the fermented product of the extract of the root-tinged mildew of the present invention fermented by using the mycelium of mushrooms of the present invention as an active ingredient, has not yet been disclosed.

The present invention has been made in view of the above-mentioned needs. In the present invention, a fermented product of a crude extract of rootstock hydrothermal extract fermented using mycelium of mulberry leaves was prepared and treated with B16F10 cells. Thereby confirming that the cytotoxic activity is substantially inhibited and the activity of tyrosinase is remarkably inhibited. Thus, the present invention has been completed.

In order to solve the above-mentioned problems, the present invention provides a cosmetic composition for whitening skin, which comprises a fermented product of a foundation stone extract as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating a melanin pigment hyperproliferative disorder, which comprises a fermented product of a cornerstone sleep extract as an active ingredient.

The present invention also provides a health functional food composition for preventing or ameliorating a melanin pigment hyperpigmentation disease, which comprises a fermented product of a cornerstone sleep extract as an active ingredient.

The fermented product of the basil remnant hydrothermal extract fermented by using the myrochsporain mushroom mycelium of the present invention has almost no cytotoxicity in comparison with the basal hydrothermal extract, and significantly inhibits the activity of tyrosinase. Therefore, cosmetics for skin whitening or melanin It can be safely and usefully used in health functional foods and medicines for prevention, improvement or treatment of hyperpigmentation diseases.

Fig. 1 shows the results of the extraction yield (%) according to the crude stone bed extracting method (ethanol and hot water).
FIG. 2 shows the results of comparative analysis of cytotoxicity of B16F10 cells treated with the fermented product of the basil remnant hydrothermal extract of the present invention and cell wall-survival rate, respectively.
Fig. 3 shows the antioxidative activity of the fermented product of the basil remnant hydrothermal extract of the present invention and the hydrothermal extract of basal stone.
FIG. 4 shows the results of comparative analysis of the inhibitory activity of the mushroom tyrosinase of the fermented product of the basil remnant hydrothermal extract according to the present invention and the hydrothermal extract of basilisk.
FIG. 5 shows the results of confirming the pH stability of the toner (A) and the lotion (B) to which the fermented product of the basil dry powdery extract of the present invention was added.
FIG. 6 shows the results of confirming the temperature cycling stability of the cosmetics to which the fermented product of the basil dry heat extract of the present invention was added.

In order to accomplish the object of the present invention, the present invention provides a cosmetic composition for whitening skin, which comprises a fermented product of a cornerstone sleep extract as an active ingredient.

In the cosmetic composition according to an embodiment of the present invention, the fermented product of the base stone sleep extract may be obtained by fermenting the base stone sleep extract with the mycelium of mushroom, but not limited thereto.

In addition, the foundation stone sleep extract may be, but not limited to, a base stone sleeping water extract.

In addition, the fermented product of the foundation stone sleep extract

1) adding 0.5 to 8 parts by weight of basil starch hydrothermal extract and 0.5 to 2.5 parts by weight of carbon source to 100 parts by weight of water, and sterilizing at 110 to 130 ° C for 10 to 20 minutes; And

2) Inoculating 5 to 15 parts by weight of the cultured Mycelium of mushroom mycelium with 100 parts by weight of the sterilized liquid and culturing the mixture at 15 to 30 ° C for 10 to 30 days to obtain a fermented product It may be a fermentation product of the base stone sleep extract obtained from the fermentation method,

Preferably, the fermentation product of the cornerstone sleep extract comprises

1) adding 5 parts by weight of basil starch hydrothermal extract, 1 part by weight of glucose and 1 part by weight of sucrose to 100 parts by weight of water, and sterilizing at 121 캜 for 15 minutes; And

2) Inoculation of 10 parts by weight of a culture solution of Mycobacterium tuberculosis mycelium to 100 parts by weight of the sterilizing solution, followed by culturing at 23 ° C for 25 days to obtain a fermented product of the base stone sleep extract, But it is not limited thereto.

In addition, the composition may inhibit the activity of tyrosinase but is not limited thereto.

In the cosmetic composition according to one embodiment of the present invention, the cosmetic composition for skin whitening can be used as an external skin ointment, a cream, a softening longevity, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing Lotions, cleansing creams, body lotions, and body cleansers. However, the present invention is not limited thereto. The cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

The cosmetic composition of the present invention may contain, in addition to the active ingredient, at least one skin whitening active ingredient exhibiting the same or similar functions. Skin whitening active ingredients include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanone, methylenedioxyphenylalkanol, 2,7-dinitroindazole or Plant extracts such as mandarin orange extract, rice extract, licorice extract, and the like, but are not limited thereto.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is a cleansing agent containing an interfacial active agent, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .

The present invention also provides a pharmaceutical composition for preventing or treating a melanin pigment hyperproliferative disorder, which comprises a fermented product of a cornerstone sleep extract as an active ingredient.

The term " melanin pigment hyperpigmentation " as used herein means darker or darker than other areas due to excessive increase of melanin in specific areas of skin or hands and toenails. The melanin pigment hyperpigmentation disorder can be selected from the group consisting of freckles, senile spots, liver, spots, brown or black spots, daylight pigmentation, cyanic melasma, hyperpigmentation after drug use, gravidic chloasma, But is not limited to, post-inflammatory hyperpigmentation due to burns or dermatitis, including burns.

The pharmaceutical composition of the present invention may contain a pharmaceutically acceptable carrier in addition to the active ingredient. Such a carrier is usually used at the time of formulation, and includes lactose, dextrose, sucrose, sorbitol, mannitol, starch, Calcium carbonate, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and minerals Oils, and the like, but are not limited thereto. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc., in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington's Pharmaceutical Sciences, 19th ed., 1995.

The appropriate dosage of the pharmaceutical composition according to the present invention may vary depending on such factors as the formulation method, the administration method, the patient's age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, . On the other hand, the dosage of the pharmaceutical composition of the present invention is preferably 0.0001 to 100 mg / kg (body weight) per day.

The pharmaceutical composition of the present invention may be administered orally or parenterally. In the case of parenteral administration, the composition may be administered topically to the skin, intravenously, subcutaneously, intramuscularly, intraperitoneally, or transdermally. In view of the fact that the pharmaceutical composition of the present invention is applied for the treatment or prevention of a melanin pigment hyperpigmentation disease, it is preferable that the composition is topically applied to the skin.

The concentration of the active ingredient contained in the composition of the present invention can be determined in consideration of the purpose of treatment, the condition of the patient, the period of time required, and the like, and is not limited to a specific range of concentration.

The pharmaceutical composition of the present invention may be manufactured in a unit dosage form by formulating it using a pharmaceutically acceptable carrier or excipient according to a method which can be easily carried out by those having ordinary skill in the art to which the present invention belongs Into a capacity container. The formulations may be formulated into any one of the formulations selected from injectables, creams, patches, sprays, ointments, alerts, lotions, liniments, pastes and cataplasms, and may additionally contain dispersing or stabilizing agents have.

The present invention also provides a health functional food composition for preventing or ameliorating a melanin pigment hyperpigmentation disease, which comprises a fermented product of a cornerstone sleep extract as an active ingredient.

The composition may be prepared in any form of powder, granule, ring, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method. The active ingredient may be suitably used depending on its intended use (prevention or improvement). Generally, the health functional food composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight based on the raw material, when the food or beverage is produced. However, in the case of long-term intake intended for health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount of more than the above range.

There is no particular limitation on the kind of the health functional food. Examples of the foods to which the health functional food composition can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, soups, Drinks, alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be produced as a food, particularly a functional food. The functional food of the present invention may contain ingredients that are conventionally added. For example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, in the case of a drink, a natural carbohydrate or a flavoring agent may be included as an additional ingredient in addition to the active ingredient. The natural carbohydrate may be selected from the group consisting of monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, , Xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., tau Martin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

In addition to the above health functional food composition, it is also possible to use various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like. Although the ratio of the above-mentioned ingredients is not critical, it is generally selected in the range of 0.01 to 0.1 part by weight based on 100 parts by weight of the health functional food composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Materials and methods

One. Foundation stone sleep Heat number  Extract Fermentation product  Manufacturing and exploring optimal conditions

The yields of groundwashing moth extracts were measured and compared by the extraction method (ethanol and hot water). As shown in FIG. 1, the yield of crude core hydrothermal extract was significantly higher than that of crude core ethanol extract.

The fermented product of the basil remnant water extract was prepared by adding 1%, 5%, 10% and 20% (w / v) of basil remoccinate extract to distilled water and adding 1% v) glucose and 1% (w / v) sucrose were added and sterilized at 121 ° C for 15 minutes. After cooling, 10 ml of the mycelium of Norfolger mushroom was inoculated and cultured for 25 days at 25 ° C and 120 rpm A fermented product of crude stone waxy hot water extract was prepared.

In order to determine the optimal temperature for fermentation of the basal hydrothermal extracts, the mycelial density was determined by adjusting the temperature to 17 ~ 26 ℃. As a result, as shown in the following Table 1, the mycelial density was highest at 23 ° C on the 15th day of culture, and the optimum temperature for fermentation of the basil removers was determined to be 23 ° C. As a result of fermentation using the mycelia of mushroom mushroom according to the addition ratios (1%, 5%, 10% and 20%) of the base stone hydrothermal extract, as shown in the following Table 2, , The fermentation efficiency was the most suitable. In case of adding 10% or more of the basal hydrothermal extract, it was confirmed that the fermentation efficiency was significantly lowered by inhibiting the growth of the mycelium.

Results of mycelial density measurement of Fermented mushroom according to fermentation temperature Temperature (℃) Mycelial density (mg / day) 17 224.7 ± 14.4 18 235.8 ± 10.5 19 243.5 ± 11.2 20 251.6 + - 7.4 21 258.8 ± 10.2 22 267.7 ± 9.7 23 281.4 ± 9.4 24 263.7 ± 9.2 25 246.1 + - 13.4 26 234.6 ± 12.1

The mycelial density measurement of the mushroom, Corvus spp. Of foundation stone sleep extract
Addition ratio (%) Mycelial density (mg / day) One 189.6 ± 9.2 5 220.8 ± 8.7 10 210.3 ± 10.6 20 178.5 ± 12.5

2. Measurement of cell viability

B16F10 mouse melanoma cells were cultured, and 1 x 10 < ⁵ > cells / ml were dispensed into each well of a 96-well plate. After incubation for 24 hours, the cells were subcultured using the basal membrane hydrothermal extract and mycelia The fermented product of the crude extract of the crude persimmon after fermentation was treated at a concentration of 10, 50, 100 and 500 μg / ml, respectively. After 24 hours of reaction, MTT (3- (4,5-dimethyldiazol- 5-decane terrazolium bromide) reagent was added to form formazan for 4 hours. At the end of the reaction, the medium was removed and the formazan was dissolved in dimethyl sulfoxide (DMSO), absorbance was measured at 540 nm using an ELISA reader, and the result was calculated as a ratio to the control value that was not treated.

3. Antioxidant activity measurement

DPPH (α, α-diphenyl-β-picrylhydrazyl) is a relatively stable free radical, which is a substance in which aromatic compounds and amines are reduced by the electron donating ability of antioxidants. (10 mg / ml) was added to 3.9 ml of a 0.05 mM DPPH solution dissolved in 99.9% methyl alcohol and mixed for 10 seconds. The mixture was incubated for 30 minutes at room temperature for 30 minutes And the absorbance was measured at 517 nm. The negative control used the same amount of distilled water as the fermented product of the cornerstone sleep extract and vitamin C (1 mg / ml), a well-known antioxidant, was used as a positive control. DPPH scavenging activity was expressed as the concentration of the sample (IC ₅₀ ), which shows a 50% absorbance reduction for the control.

4. Mushroom (mushroom) inhibitory activity of tyrosinase

Tyrosinase is an enzyme involved in the synthesis of melanin, resulting in continuous enzymatic oxidation of L-tyrosine (DOPA) in the body and subsequent conversion to L-Dopaquinone Freckles and stains.

Mushroom Inhibitory activity of tyrosinase was measured by adding 0.2 ml of mushroom tyrosinase (200 mg) to a mixed solution of 0.2 ml of a 10 mM L-DOPA substrate and 0.1 ml of a sample solution in 0.5 ml of 0.175 M phosphate buffer (pH 6.8) U / ml) was added, mixed for 10 seconds, reacted at 25 ° C for 2 minutes, and then the absorbance of the resultant DOPA Chrome was measured at 475 nm. Mushroom tyrosinase inhibition (%) was And was calculated by the following formula.

Example  1. The present invention Mushroom mushroom  Fermented using mycelium Foundation stone sleep Heat number  Extract Fermented  Confirm cell viability

In Example 1, B16F10 cells were treated with the fermented product of the crude extract of basilica coelomicum and fermented by using the mycelia of the present invention of the present invention at 10, 50, 100 and 500 μg / ml, respectively, for 24 hours After the reaction, cell viability was measured. As a result, as shown in FIG. 2, the cell survival rate was well maintained when 10 to 100 μg / ml of crude extracts and 10% to 100 μg / ml of fermented product were treated. However, 500 μg / , It was confirmed that the cell survival rate was reduced by about 30% when treated. On the other hand, when the fermented product of 500 占 퐂 / ㎖ of basal hydrothermal extract was treated, the cell survival rate was reduced by about 10%, and the cell viability was maintained well.

Example  2. The present invention Mushroom mushroom  Fermented using mycelium Foundation stone sleep Ten Identification of Antioxidant Activity of Fermented Extracts

The antioxidative activities of the fermented water and the basic hydrothermal extracts of the crude hydrothermal extracts were investigated and compared. As a result, also the foundation sleep hot-water extract was antioxidant activity is better in case of foundation sleep ethanol extract as disclosed in the third, the antioxidant is fermented in cornerstones sleep hot water extract than the foundation sleep hot water extract lowered to the IC ₅₀ values significantly Activity was more excellent.

Example  3. The Mushroom mushroom  Fermented using mycelium Foundation stone sleep  Of hot-water extract Fermentation product  Depending on the treatment Mushroom  Mushroom tyrosinase ) Inhibitory activity

The inhibitory activity of the mushroom tyrosinase of the basil remnant hydrothermal extract and basil remnant hydrothermal extract fermented by using the mycelium of mushroom mycelium of the present invention was measured. As a result, as shown in FIG. 4, in addition to the effect of inhibiting the activity of tyrosinase at all concentrations of the fermented product of the basil dry powdery extract of the present invention, It was confirmed that about 54% inhibition of tyrosinase activity was observed when the fermented product of udug / ml of crude basic hydrothermal extract was treated.

Example  4. The Mushroom mushroom  Fermented using mycelium Foundation stone sleep Heat number  Extract Fermented water  Preparation and stability evaluation of added prototype

In Example 4, toners and lotions containing a fermentation product (hereinafter referred to as a "raw material") of the base-coherent hydrothermal extract of the present invention were prepared and their stability evaluations were carried out.

1) pH stability

The pH of the cosmetic was measured by a pH meter manufactured by Corning (pH meter440, Corning, USA), and the pH value of the toner and lotion added with the fermented product of the base stone hydrothermal extract stored at 25 ° C was measured. Before the measurement, the glass electrode was calibrated with a buffer solution of pH 4 and pH 7 beforehand, and the detector was washed with distilled water and lightly wiped off. The glass electrode was stored for 30 days. As a result of measuring the pH value at 1 day, 3 days, 5 days, 7 days, 15 days and 30 days while storing the prototype with the fermented product of rootstock hydrothermal extract at 25 ° C, the toner showed a pH of 5.77 ± 0.03, The pH was 6.34 ± 0.03, indicating that no significant change was observed over time (FIG. 5).

2) Viscosity measurement

Viscosity measurements of cosmetics were measured using a Brookfield LV (Brookfield Engineering Laboratories, Inc., USA). Spindle No. 64 was selected and measured at 6 rpm for 30 seconds and defoamed during manufacturing to reduce the effect of bubbles. The viscosities of the toners and lotions to which the fermented product of the crude core hydrothermal extract stored at 25 ° C was added were measured. The results were repeated three times and stored for 30 days. As a result, on average, the toner showed 15,000 ~ 18,000 mPa and the lotion 26 ~ 28,000 mPa · s. As a result of measuring the viscosity with time, no significant change was observed.

3) Measurement of stability according to temperature

The stability test according to temperature was carried out at 5 ° C, 25 ° C and 40 ° C, and the condition of toner and lotion changes with time. In the evaluation of stability according to temperature, coagulation, precipitation, trajectory and separation were observed at low temperature (5 ℃), and rancidity, discoloration, detachment, viscosity, precipitation and turbidity were observed at high temperature (40 ℃). As a result, the stability of the toner and lotion added with the fermented product of cornstarch hydrothermal extract was not changed for 30 days, and the stability was confirmed according to the temperature.

4) Stability measurement according to cycle chamber

In order to observe the changes in physical properties of cosmetics according to the storage conditions of low temperature and high temperature in four seasons in Korea, the toner and lotion were stored in transparent containers at -15, -10, 5, 10, 25 and 40 ℃, The physical changes were visually observed with a cycle. As a result, it was confirmed that the toner and the lotion to which the fermented product of the foundation stone hydrothermal extract was added did not change for 30 days (FIG. 6).

5) Stability measurement by freeze-thaw cycling

In the stability test according to the cooling and thaw cycling, the toner and lotion were placed in a transparent container and stored at -10 ° C. and 25 ° C. for 24 hours, respectively, and the stability for 3 and 7 days was visually observed for 1 cycle . As a result, it was shown that both the toner and the lotion added with the fermented product of the crude stone waxy hydrothermal extract were stable, and it was stable without separation of the phases in both the 3 cycle and the 7 cycle.

Claims (8)

A cosmetic composition for skin whitening comprising as an active ingredient a fermented product of a rootstock hydrothermal extract fermented using a mycelium of Aspergillus oryzae. delete delete 2. The method according to claim 1, wherein the fermentation product of the basal-
1) adding 0.5 to 8 parts by weight of basil starch hydrothermal extract and 0.5 to 2.5 parts by weight of carbon source to 100 parts by weight of water, and sterilizing at 110 to 130 ° C for 10 to 20 minutes; And
2) Inoculation of 5-15 parts by weight of mycelium culture solution with 100 parts by weight of the sterilization solution and culturing at 15-30 ° C for 10-30 days to obtain a fermentation product of crude core hydrothermal extract Wherein the fermented product is a fermented product of a crude hardening hot water extract obtained from a fermentation method. The cosmetic composition for skin whitening according to claim 1, wherein the composition inhibits the activity of tyrosinase. A pharmaceutical composition for preventing or treating a melanin pigment hyperpigmentation disease, comprising as an active ingredient a fermented product of a rootstock hydrothermal extract fermented using a mycelium of mushrooms. 7. The method of claim 6, wherein the melanin pigment hyperpigmentation disorder is selected from the group consisting of freckles, aging spots, liver, spots, brown or black spots, daylight pigmentation, cyanic melasma, hyperpigmentation after drug use, chloasma), or hyperpigmentation after inflammation due to scratches or dermatitis, including scratches and burns. A health functional food composition for preventing or ameliorating a melanin pigment hyperpigmentation disease containing as an active ingredient a fermented product of a rootstock hydrothermal extract fermented using a mycelium of mushrooms.

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