KR101858118B1 - Composition for skin whitening comprising fermented Geomgangsong as effective component - Google Patents

Abstract

The present invention relates to a composition for whitening skin, which comprises a fermented product of a goldsmith carrier as an active ingredient. Specifically, the fermented product of the gold-spirited carrageenan is a fermented product of primary fermentation of a fermented product of fermented milk or a fermented product of a fermented product of alcohol, , A functional cosmetic for skin whitening, and a health functional food or skin pigmentation disease which can prevent or improve skin pigmentation disease.

Description

Technical Field [0001] The present invention relates to a skin whitening composition comprising a fermented product as an active ingredient,

The present invention relates to a composition for whitening skin, which comprises a fermented product of a goldsmith carrier as an active ingredient.

The skin may be exposed to various harmful substances such as air and ultraviolet ray caused by air pollution and may cause various skin troubles. Among them, dandruff, freckles, and dandruff are among the biggest skin problems, and many efforts have been made to improve them.

Several factors are involved in determining the skin color of a person. Among them, the activity of melanocyte which makes melanin pigment, distribution of blood vessels, thickness of skin, carotenoid, bilirubin and other factors related to pigment are important. In particular, the most important factor is melanin, which is produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. Skin pigmentation is caused by stimuli such as ultraviolet rays or inflammation, and it is also known to be caused by factors such as genetic factors, hormones, foods, and chemicals. In particular, inflammation related factors such as prostaglandins, histamine, alpha-MSH, and NO (nitric oxide) have been implicated in skin pigmentation abnormalities such as spots, freckles and pigmentation, and melanocytes ) Is known to activate melanin production. It is also reported that hyperpigmentation is accompanied by immunological mediators in tissue damage and regeneration process. When melanocytes are stimulated by various factors, tyrosinase produces melanin in the melanosome. As a result, melanocytes are melanosomes. That is, tyrosine in melanocytes is transformed into dopa by the action of tyrosinase and then transformed into dopaquinone by the action of dopaoxidase, and then tyrosinase-related related protein-2 (hereinafter referred to as TRP-2) and tyrosinase-related protein-2 (hereinafter referred to as TRP-2) to produce melanin. In this series of reactions, tyrosinase activates the rate-limiting reaction of the melanin pigmentation process, so that melanogenesis is primarily controlled by the activity and expression of tyrosinase. TRP-2, also known as the DOPA chromium mutant enzyme, activates the reaction of DOPA chromium with 5,6-dihydroxyindole-2-carboxylic acid (DHICA), TRP-1 activates DHICA with carboxylindole- . As a result, TRP-1 and TRP-2 are involved in the polymerization reaction, which is the final step in the formation of the insoluble pigment melanin. It has been reported that tyrosinase, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2) are activated by inflammatory mediators to increase the synthesis of melanin .

Currently developed whitening agents include kojic acid, arbutin, glutathione and vitamin C, but consumers do not have a satisfactory whitening effect and their use is limited due to skin safety. Thus, various attempts have been made to synthesize melanin.

On the other hand, pine trees and pine needles have been known to have antimicrobial, sterilizing and deodorizing functions from the past, and Kumkansong, which is also called "Chungyangmok", is known to have excellent deodorizing action due to its unique wood structure. As an example of a technique relating to cosmetics, health functional foods or pharmaceutical compositions containing pine as an active ingredient, the natural fermented pine leaf extract disclosed in Korean Patent Publication No. 2015-0086922 has a composition for relieving skin irritation and skin moisturizing, Patent No. 2016-0038228 discloses a skin whitening composition containing an extract of pine cones as an active ingredient and Korean Patent No. 1608258 discloses a cosmetic composition for moisturizing skin containing a rosin extract, But no description has been made about a composition for skin whitening containing the fermented product of the present invention as an active ingredient.

The present invention provides a skin whitening composition comprising a fermented product as an active ingredient, and the fermented product of the gold-smelting carrier, which is an effective ingredient, is melanoma cells (B16-F10 mouse melanoma cell, the present inventors have accomplished the present invention by confirming that it has an effect of inhibiting the activity of tyrosinase as well as effectively suppressing the production of melanin, compared with the culture broth before fermentation.

In order to solve the above-mentioned problems, the present invention provides a cosmetic composition for whitening skin, which comprises a fermented product of the present invention as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing or treating a melanin pigment hyperproliferative disorder, which comprises a fermented product of a goldsmith carrier as an active ingredient.

The present invention also provides a health functional food composition for prevention or amelioration of hyperpigmentation of melanin pigment comprising a fermented product as a active ingredient.

The present invention relates to a composition for whitening skin, which comprises a fermented product of a goldsmith carrier as an active ingredient. Specifically, the fermented product of the gold-spirited carrageenan is a fermented product of primary fermentation of a fermented product of fermented milk or a fermented product of a fermented product of alcohol, , A functional cosmetic for skin whitening, and a health functional food or skin pigmentation disease which can prevent or improve skin pigmentation disease.

FIG. 1 schematically shows a manufacturing process of a gold-smelting fermentation product of the present invention.
FIG. 2 is a graph showing the difference in the sugar content and the alcohol content of the fermented alcohol according to the addition amount of the poultry meal powder according to an embodiment of the present invention.
FIG. 3 is a graph showing the degree of sugar content and alcohol content of an alcoholic fermented product according to addition of sugar (A), apple concentrate (B) and brown rice (C) This is the result of confirming the difference.
FIG. 4 is a graph showing changes in sugar content and alcohol production of alcohol fermented according to the initial sugar content (12 to 16 Bricks) of the culture media of Kumkang River according to an embodiment of the present invention.
FIG. 5 is a graph showing changes in sugar content and titratable acidity of acetic acid fermented product according to addition of sugar (A), apple concentrate (B) and brown rice (C) titratable acidity.
FIG. 6 shows the results of confirming titratable acidity and alcohol content changes of the acetic acid fermented product according to acidity difference (1 to 3%) before acetic acid fermentation according to an embodiment of the present invention.
FIG. 7 is a graph showing the relationship between the content (A) of melanin in melanocytes of B16F10 mouse and the content (A) of melanocytes in B16F10 mouse melanocyte cells treated with α-MSH (α-melanocyte stimulating hormone) And the activity (B) of the cinacalcin was measured. α-MSH is a melanogenesis inducer, and AR is a positive control for arbutin.

The present invention relates to a cosmetic composition for skin whitening comprising a gold-smelting fermented product as an active ingredient.

The fermented product of the present invention is preferably fermented by a fermented tea leaf or a powder of a branch, but is not limited thereto. The gold-smelting fermentation product is characterized by inhibiting the production of melanin or the activity of tyrosinase. The fermented fish product of the present invention can be obtained by (1) culturing Saccharomyces cerevisiae cerevisiae , and (2) fermenting the fermented product obtained in (1) above to a fermented product of Acetobacter but it is not limited thereto. Any of the strains fermenting alcohol or fermenting acetic acid may be selected and used. Preferably, the fermentation liquid includes 5 to 30% by weight of the pneumoconiotic powder, and more preferably 15% by weight of the pneumoconiotic powder. However, the present invention is not limited thereto.

The culture medium for suspension culture may further contain sugar, an apple concentrate or a brown rice in addition to the pest control powder, preferably an apple concentrate, but the present invention is not limited thereto.

Preferably, the initial sugar content of the cultured poultry culture medium is 3 to 16 Bricks, more preferably 12 Bricks, but the present invention is not limited thereto. The initial acidity of the acetic acid fermentation is 1 to 3%, but is not limited thereto.

The alcohol fermentation and the acetic acid fermentation are preferably performed at 28 to 32 ° C. for 3 to 5 days, more preferably at 30 ° C. for 4 days for fermentation of alcohol and acetic acid.

On the other hand, the whitening effect refers to an effect to improve or prevent glaring skin, spots, freckles, and dark circles caused by various factors such as exposure to ultraviolet rays, change in hormone balance, genetic program, Or an effect of maintaining a beautiful skin with a sense of transparency and an effect of reducing the dullness of the skin to increase gloss and tautness. In general, it is known that the skin, spots, freckles, and dark circles of the skin, stamens, freckles and dark circles are stimulated by melanocytes due to stimulation by ultraviolet rays or changes in hormone balance, and melanin pigment is deposited on the skin. Therefore, if the production of melanin can be inhibited, it is possible to prevent or improve gross skin, spots, freckles and dark circles.

The cosmetic composition for skin whitening may be a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing oil, a powder foundation, an emulsion foundation, a wax foundation and a spray , But the present invention is not limited thereto. The cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

The cosmetic composition of the present invention may contain, in addition to the active ingredient, at least one skin whitening active ingredient exhibiting the same or similar functions. Skin whitening active ingredients include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanone, methylenedioxyphenylalkanol, 2,7-dinitroindazole or Plant extracts such as mandarin orange extract, rice extract, licorice extract, and the like, but are not limited thereto.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is a cleansing agent containing an interfacial active agent, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .

The present invention also relates to a pharmaceutical composition for preventing or treating a melanin pigment hyperproliferative disorder, comprising a fermented product as an active ingredient. In addition to the above-mentioned fermentation product for a goldsmith, it is preferable to further include a carrier, an excipient or a diluent, but is not limited thereto.

As used herein, the term " melanin pigment hyperpigmentation "means darkening or darkening compared to other areas due to excessive increase of melanin in specific areas of skin or hands and toenails.

The melanin pigment hyperpigmentation disorder can be selected from the group consisting of freckles, senile spots, liver, spots, brown or black spots, daylight pigmentation, cyanic melasma, hyperpigmentation after drug use, gravidic chloasma, Post-inflammatory hyperpigmentation due to burns or dermatitis including burns, and the like.

The pharmaceutical composition of the present invention may be various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like. Examples of suppositories include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and it is preferable to select the intraperitoneal, rectal, rectal, intravenous, intramuscular, subcutaneous, intrauterine or intracerebral injection methods during parenteral administration, It is preferably used for external skin application.

The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will depend on the type of disease, severity, , Sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts. The composition of the present invention can be administered as an individual therapeutic agent or in combination with other therapeutic agents, and can be administered sequentially or simultaneously with conventional therapeutic agents, and can be administered singly or in multiple doses. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.

The dosage of the pharmaceutical composition of the present invention may be varied depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and severity of the disease.

The present invention also provides a health functional food composition for prevention or amelioration of hyperpigmentation of melanin pigment comprising a fermented product as a active ingredient.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method. The amount of the active ingredient can be suitably used depending on its use purpose (prevention or improvement). Generally, the health functional food composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight based on the raw material, when the food or beverage is produced. However, in case of long-term ingestion for health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the health functional food. Examples of the foods to which the health functional food composition can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, soups, Drinks, alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be produced as a food, particularly a functional food. The functional food of the present invention includes components that are ordinarily added in food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, in the case of a drink, a natural carbohydrate or a flavoring agent may be included as an additional ingredient in addition to the active ingredient. The natural carbohydrate may be selected from the group consisting of monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, , Xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., tau Martin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

In addition to the above health functional food composition, it is also possible to use various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

[Materials and Methods]

1. Materials

The Kumkang shrimp by - product (Kumkwang powder) was purchased from Uljin Forestry Cooperative, and the apple concentrate was purchased from Elim Cai and used as raw material.

2. Use strain and medium composition

Alcohol fermentation strain Saccharomyces cerevisiae was subcultured in YPD plate medium and cultured in YPD liquid medium.

Acetic acid fermentation strains were obtained from Acetobacter sp.), and the composition of the culture medium is shown in Table 1 below.

 Culture medium culture of acetic acid bacteria Composition ratio(%) Yeast extract 0.5 Glucose 0.5 glycerin 1.0 MgSO ₄ .7H ₂ O 0.02 ethanol 5.0 Acetic acid 1.0 water 91.98

3. Preparation of Kumhwang Thrapen by-product (Kumhwang powder)

The collected ginseng shrimp by - products (leaves and branches) were washed and immersed in water. After the water was removed well, it was pulverized by crushing, pulverized, dried and used for fermentation.

4. Alcohol fermentation conditions

1) Alcohol fermentation according to the content of Kumhang shim by-product

The apple concentrate (70 ° brix) was diluted in purified water and adjusted to a sugar degree of 12 ° brix. The fermentation broth containing 5, 10, 15 and 30% (w / v) Saccharomyces S. cerevisiae were inoculated and fermented at 30 DEG C for 96 hours.

2) Alcohol fermentation according to kinds of additives (sugar, apple concentrate and brown rice)

When alcohol fermentation, sugar, brown rice, and apple concentrate were added and the initial sugar content was adjusted to 3 ~ 12 Bricks and fermented for 96 hours. The sugar content and alcohol content were measured at 24 hour intervals.

3) Alcohol fermentation according to initial sugar content

The initial sugar content of the fermentation broth was adjusted to 12 ~ 16 ° brix and the sugar content and alcohol content were measured at 24 - hour intervals.

5. Acetic acid fermentation conditions

1) Acetic acid fermentation according to kinds of additives (sugar, apple concentrate and brown rice)

To the fermentation broth of alcohol fermented by adding sugar, apple concentrate and brown rice to 15% (w / v) shrimp by - product (Kumunggwang powder), acetic acid bacteria Acetobacter sp.) and incubated at 30 캜 for 180 hours at 180 rpm for acetic acid fermentation.

2) Optimum conditions of acetic acid fermentation of Kumhang shim by-product

In order to investigate the optimal acetic acid fermentation condition of Kumkang shrimp by - product (Kumkwang powder), the initial acidity of the end - of - fermentation solution was adjusted to 1 ~ 3% and the alcohol content was changed at 24 - Acetic acid content was measured.

Example  1. Establishment of alcohol fermentation condition according to the contents of Kumhwang's thinning by-product

5, 10, 15 and 30% (w / v) gangshang thinning by-products (Kum-gang Song powder) were added to the fermentation broth containing 12 Bricks of apple concentrate as an initial condition for alcohol fermentation of Kum- , Celebi as Saccharomyces My jiae access (Saccharomyces S. cerevisiae were inoculated and fermented at 30 DEG C for 96 hours.

As a result, the sugar content decreased and the alcohol content increased with time in all experimental groups. In particular, the alcohol content was highest when 15% (w / v) gangshang thinning by-product (Kumkwang powder) was added to the final product, and 30% (w / v) In one case, the alcohol fermentation did not occur well, and the alcohol content was found to be the lowest (Fig. 2). From these results, it can be concluded that the concentration of by - product of Kum - gum shrimp (Kum - shong powder) is too high and the fermentation does not progress well.

Example  2. Alcohol fermentation according to kinds of additives

In order to investigate the effect of additives on the alcohol fermentation of alcoholic fermentation of the shrimp by - product (Kumkwang powder), 10% (w / v) , Apple concentrate (12 bricks) or brown rice (3 bricks) were added to perform alcohol fermentation.

As a result, it was confirmed that alcohol fermentation proceeded normally in all experimental groups, and alcohol content was highest when alcohol concentrate was added in the alcohol fermentation with addition of sugar, apple concentrate or brown rice (FIG. 3).

Example  3. Ganghwangsun thinning by-product according to the initial sugar content (Kumkangshon powder) of  Alcohol fermentation

In order to investigate the effect of initial sugar content on fermentation during alcohol fermentation, 15, 12, 14, and 16 brix apple concentrates were added to the shrimp by - product of 15% (w / v) And the fermentation was carried out after controlling the initial sugar content.

As shown in FIG. 4, alcohol fermentation proceeded normally in all groups, and the alcohol content was increased in proportion to the initial sugar content, but the difference was not significant.

Therefore, considering fermentation efficiency and economical aspect, it was judged that fermentation with 12 brix apple concentrate is most suitable.

Example  4. Acetic acid fermentation of Kumkang shim by-product (Kumkwang powder) according to the kind of additives

In order to investigate the effect of supplementary ingredients on the acetic acid fermentation during the acetic acid fermentation using alcohol obtained by alcohol fermentation of 15% (w / v) shrimp by - product (Kumunggwang powder) The concentrate or brown rice was added to conduct alcohol fermentation.

As a result, it was confirmed that the acetic acid fermentation proceeded normally in all the experimental groups. When the sugar, the apple concentrate or the brown rice was added, the acetic acid fermentation was performed after the alcohol fermentation, when the apple concentrate was added, (Fig. 5).

Example  5. Establishment of Acetic Acid Fermentation Condition of Kumkang Stream Thumbnail By-product (Kumkang Song Powder) according to Initial Acidity

Initial acidity was adjusted to 1, 2, 3% (v / v) in order to investigate the effects of initial acidity on the acetic acid fermentation of Kumkang shrimp by - product (Kumkwang powder).

As a result, it was confirmed that the acetic acid fermentation proceeded smoothly at 1% (v / v) and the fermentation rate was slower than 1% (v / v) at 2 and 3% (v / v) ).

Example  6. Ganghwangsun thinning by-product (Kumkwang powder) Fermented  Produce

On the basis of the results of Examples 1 to 5, 15% (w / v) Kumung Shipping Powder and 12 Bricks of apple concentrate were added to perform alcohol fermentation for 4 days, initial acidity was adjusted to 1% The fermentation was carried out to confirm that the acidity was the most effective.

Example  7. In the present invention, Fermented  The production of melanin and the production of tyrosinase (< RTI ID = 0.0 > tyrosinase ) ≪ tb > <

The biosynthesis of melanin is carried out by using tyrosin, which is a kind of amino acid, as a precursor, through DOPA, DOPA quinone and indole-5,6-dihydroquinone, -5,6-dihydroquinone. ≪ / RTI > Melanin is produced in the melanocyte and is transferred to the epidermis and dermis boundary in the form of granules called melanosomes. Melanin is not only a direct cause of dark skin, but also causes serious problems in terms of skin beauty, such as promoting the formation of spots and freckles. In addition, over-producing melanin has been found to cause skin aging and skin cancer.

Therefore, the content of melanin and the activity of tyrosinase according to the treatment of the fermented product of the present invention were measured. As a result, the production of melanin was induced by treating? -MSH as shown in FIG. , The amount of melanin produced was reduced when the kojang fermented product of the present invention (acetic acid fermentation) was treated. In particular, when 400 μg / ml fermented kojang (acetic acid fermented) was treated, about 60% of melanin . In addition, the activity of tyrosinase was similarly inhibited as a result of treatment of fermentation product of acetic acid (acetic acid fermentation). In particular, when fermented product of 400 μg / % Of tyrosinase activity and showed more inhibitory effect than arbutin, a positive control.

Claims (12)

(1) A Saccharomyces cerevisiae strain is inoculated to a culture medium containing 5 to 30% by weight of a mixture of a gangbuwa leaf and an eggplant mixture, fermented at 28 to 32 ° C for 3 to 5 days, (2) A skin-containing fermented product obtained by fermenting acetic acid for 3 to 5 days at 28 to 32 ° C by inoculating an Acetobacter sp. Strain into the fermented product obtained in the above step (1) A cosmetic composition for whitening. delete [Claim 3] The cosmetic composition for skin whitening according to claim 1, wherein the kojang fermented product inhibits the production of melanin or tyrosinase activity. delete delete [Claim 3] The cosmetic composition for skin whitening according to claim 1, wherein the culture medium for smoothening further comprises at least one selected from sugar, apple concentrate and brown rice in addition to a pearl powder. The cosmetic composition for skin whitening according to any one of claims 1 to 3, wherein the initial amount of the saccharide culture fluid is 3 to 16 Bricks. The cosmetic composition for skin whitening according to claim 1, wherein the initial acidity of the acetic acid fermentation is 1 to 3%. delete (1) A Saccharomyces cerevisiae strain is inoculated to a culture medium containing 5 to 30% by weight of a mixture of a gangbuwa leaf and an eggplant mixture, fermented at 28 to 32 ° C for 3 to 5 days, (2) A fermented acuminate fermented product obtained by fermenting acetic acid for 3 to 5 days at 28 ~ 32 ° C inoculated with the Acetobacter sp. Strain in the fermented product obtained in the above (1) A pharmaceutical composition for the prevention or treatment of hyperpigmentation disorders. 11. The method of claim 10, wherein the melanin pigment hyperpigmentation disorder is selected from the group consisting of freckles, aging spots, liver, spots, brown or black spots, daylight pigmentation, cyanic melasma, hyperpigmentation after drug use, chloasma), or hyperpigmentation after inflammation due to scratches or dermatitis, including scratches and burns. (1) A Saccharomyces cerevisiae strain is inoculated to a culture medium containing 5 to 30% by weight of a mixture of a gangbuwa leaf and an eggplant mixture, fermented at 28 to 32 ° C for 3 to 5 days, (2) A fermented acuminate fermented product obtained by fermenting acetic acid for 3 to 5 days at 28 ~ 32 ° C inoculated with the Acetobacter sp. Strain in the fermented product obtained in the above (1) A health functional food composition for preventing or ameliorating hyperpigmentation diseases.

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