KR102539089B1 - Composition for skin whitening and anti-oxidant comprising fermented product of Angelica dahurica extract as effective component - Google Patents

Abstract

The present invention relates to a composition for skin whitening and antioxidant containing a fermented product of white paper extract as an active ingredient. , Since the tyrosinase activity inhibitory effect is excellent, the composition of the present invention containing the fermented product of white paper extract as an active ingredient is a functional cosmetic for skin whitening and antioxidant, a health functional food for skin whitening and antioxidant, or a treatment for melanin hyperpigmentation disease. It can be useful as a therapeutic agent.

Description

Composition for skin whitening and anti-oxidant comprising fermented product of Angelica dahurica extract as effective component}

The present invention relates to a composition for skin whitening and antioxidant containing, as an active ingredient, a fermented product of a white paper extract fermented using ericium erinaceus mycelium.

The skin is directly exposed to the external environment among the body tissues, and acts as a barrier between the inside and the external environment of the human body to protect the living body from the surrounding environment by defending against the invasion of external environmental pollutants and microorganisms, including chemicals and ultraviolet rays. .

In addition, skin color is determined by melanin, hemoglobin, carotene, etc. Among these, melanin plays the most important role. In addition to determining human skin color, melanin also performs skin protection functions such as ultraviolet ray absorption and free radical scavenger. However, when melanin is excessively produced due to external environmental changes such as excessive exposure to ultraviolet rays, air pollution, and stress, it causes pigmentation in the skin, causing melanism, melasma, and freckles.

Blackening of the skin is caused by the reaction of skin cells to internal and external factors, and a representative factor is exposure to ultraviolet rays. That is, when the skin is exposed to ultraviolet rays, tyrosinase is activated. By an oxidation process in which the tyrosinase acts on tyrosine present in skin tissue to produce DOPA and dopaquinone, A polymer called melanin is synthesized in melanosomes in melanocytes, which are skin pigment cells, and this melanin is transferred to keratinocytes, keratinocytes of the skin, and reaches the skin surface through the keratinization process. It protects the skin from UV rays. Therefore, melanin is an indispensable UV protection agent for the human body, and also serves as an effective free radical scavenger that removes various radicals that modify biological components such as proteins, lipids, and nucleic acids. However, when melanin is locally excessively synthesized or when the physiological function of the skin is deteriorated due to skin lesions and aging, melanin is deposited on the skin surface to cause melasma, freckles, and various pigmentation.

As the cause and mechanism of skin darkening have been revealed as described above, in the manufacture of whitening cosmetics, substances having an activity inhibitory effect on tyrosinase, an enzyme involved in the skin darkening process, are formulated into cosmetics, or some of them in the melanin production process. A method of reducing the production of melanin by inhibiting the reaction is generally used. Representative materials used for this purpose include chemical substances such as ascorbic acid, kojic acid, and hydroquinone, and plant extracts such as moth extract and licorice extract. However, ascorbic acid lacks tyrosinase activity inhibitory effect and its molecular stability is low, so it is not suitable as a melanogenesis inhibitor, and kojic acid has excellent tyrosinase inhibitory activity, but discolors and changes over time when formulated in cosmetics. Therefore, there is a problem in stability such as a decrease in titer, and there is a limit in use due to large skin irritation, and hydroquinone is limited in use in cosmetics due to skin irritation and safety problems. Therefore, attention is focused on the development of novel and effective whitening ingredients.

On the other hand, white paper is a perennial herbaceous plant belonging to the umbel family, Angelica dahurica . Or, it is the root of Angelica dahurica var. formosana .

The representative pharmacological action of white paper is antibacterial action against Escherichia coli, Shigella bacillus, and Influenza bacillus, and exhibits stimulating action on the vasomotor center, respiratory center, and vagus nerve. A large amount of extract causes tonic convulsions and general paralysis. The weakness is mild, and the taste is spicy. It is effective for severe headaches caused by colds or postpartum colds and headaches, and also shows pain relief for trigeminal neuralgia and toothache. In addition, it is used as an auxiliary drug when sinusitis is severe and headache is accompanied by sinusitis. The skin of the inner fruit of Baekji is made of soft cells, and there are fibrous layers and glandular ducts under the outer rind, which contain essential oils with a good fragrance. It is the same scent that can be smelled in Angelica and Cnidium. Thanks to this, it can be made into herbal scented bags and used as an air freshener.

On the other hand, ericium erinaceum is a mushroom belonging to the Coral Mushroom family, which occurs in dead trees or live trees of broad-leaved trees in autumn, and is known as monkey head mushrooms and bear head mushrooms in China, Yamabushitake in Japan, and us In Nara, it is known as deer mane mushroom because it resembles the shape of hair of deer mane. These ericium erinaceus mushrooms are rich in carbohydrates, proteins, amino acids, enzymes, inorganic salts and vitamins, and are known to have anticancer and immune function enhancing effects, and recently contain various physiologically active substances including components with anticancer effects. It has been reported to be doing.

Meanwhile, Korean Patent No. 1103283 discloses a fermented aloe product for whitening cosmetic material and whitening cosmetics using the same, and Korean Patent Publication No. 2004-0090114 discloses a composition for external application for skin containing a ericium erinaceus extract. There is no disclosure of a composition for skin whitening and antioxidant containing, as an active ingredient, a fermented product of a white paper extract fermented using the ericium erinaceus mycelium of the present invention.

The present invention has been derived from the above needs, and provides a skin whitening and antioxidant composition containing, as an active ingredient, a fermented product of a white paper extract fermented using ericium erinaceus mycelium, and the active ingredient, ericium erinaceus The present invention was completed by confirming the skin whitening effect and antioxidant effect of the fermented white paper extract fermented using mycelium.

In order to solve the above problems, the present invention provides a cosmetic composition for skin whitening and antioxidant containing a fermented product of white paper extract as an active ingredient.

In addition, the present invention provides a health functional food composition for skin whitening and antioxidant containing fermented white paper extract as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for preventing or treating hypermelanin hyperpigmentation disease containing a fermented product of white paper extract as an active ingredient.

The present invention relates to a composition for skin whitening and antioxidant containing a fermented product of white paper extract as an active ingredient. , The tyrosinase activity inhibitory effect was excellent.

1 is a result of confirming the extraction yield according to the white paper extraction method (ethanol or hot water). Different letters a to c in the figure mean that there is a statistically significant difference, p<0.05.
2 is a result of confirming the cytotoxicity of the fermented product of the hot-water extract of white paper and the hot-water extract of white paper in B16F10 cells. Different letters a to c in the figure mean that there is a statistically significant difference, p<0.05.
3 is a result of confirming the antioxidant activity of the fermented product of the white paper hot water extract and the white paper hot water extract of the present invention. Different letters a to d in the figure mean that there is a statistically significant difference, p<0.05.
Figure 4 is the result of confirming the tyrosinase inhibitory activity of the fermented product of the white paper hot water extract and the white paper hot water extract of the present invention. Different letters a to e in the figure mean that there is a statistically significant difference, p<0.05.
5 is a result of confirming the pH stability of the toner (A) and the lotion (B) to which the fermentation product of the white paper hot water extract of the present invention was added.

In order to achieve the object of the present invention, the present invention white paper ( Angelica dahurica ) Provides a cosmetic composition for skin whitening and antioxidant containing a fermented product of extract as an active ingredient.

The fermented product of the white paper extract may be fermented white paper extract using ericium erinaceus mycelium, but is not limited thereto.

The extraction solvent of the white paper extract may be water, C ₁ ~ C ₄ lower alcohol or a mixture thereof, preferably water or ethanol, more preferably water, but is not limited thereto.

The fermented product of the white paper extract is

1) Adding 1 to 30 parts by weight of white paper hot water extract and 0.5 to 2.5 parts by weight of a carbon source based on 100 parts by weight of water and sterilizing at 110 to 130 ° C. for 10 to 20 minutes; and

2) inoculating 5 to 15 parts by weight of ericium erinaceus mycelium with respect to 100 parts by weight of the sterilized solution of step 1) and culturing at 15 to 30 ° C for 10 to 30 days; can,

preferably

1) sterilizing at 110 to 130 ° C. for 10 to 20 minutes by adding 1 to 10 parts by weight of white paper hot water extract and 0.5 to 2.5 parts by weight of a carbon source to 100 parts by weight of water; and

2) inoculating 5 to 15 parts by weight of ericium erinaceus mycelium with respect to 100 parts by weight of the sterilized solution of step 1) and culturing at 22 to 25 ° C for 10 to 30 days; can,

more preferably

1) Adding 5 to 10 parts by weight of white paper hot water extract, 0.8 to 1.2 parts by weight of glucose and 0.8 to 1.2 parts by weight of sucrose based on 100 parts by weight of water and sterilizing at 115 to 125 ° C. for 13 to 17 minutes; and

2) inoculating 10 parts by weight of ericium erinaceus mycelium with respect to 100 parts by weight of the sterilized solution of step 1) and incubating at 22 to 24 ° C for 15 to 28 days; ,

more preferably

1) sterilizing at 121° C. for 15 minutes by adding 5 parts by weight of white paper hot water extract, 1 part by weight of glucose and 1 part by weight of sucrose to 100 parts by weight of water; and

2) inoculating 10 parts by weight of ericium erinaceus mycelium with respect to 100 parts by weight of the sterilized solution of step 1) and culturing at 23 ° C for 25 days; It is not.

In one embodiment of the present invention, the fermented product of the white paper extract, which is the active ingredient of the present invention, is characterized in that the cytotoxicity is alleviated compared to the white paper extract.

In the cosmetic composition according to one embodiment of the present invention, the cosmetic composition for skin whitening and antioxidation is an external skin ointment, cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, rinse, hair conditioner, hair treatment Treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam , It may be prepared in any one formulation selected from the group consisting of cleansing lotion, cleansing cream, body lotion and body cleanser, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and additives necessary and appropriate for formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

The cosmetic composition of the present invention may further contain at least one skin whitening active ingredient exhibiting the same or similar function in addition to the active ingredient. Skin lightening active ingredients include kojic acid and its derivatives, arbutin, ascorbic acid and its derivatives, hydroquinone and its derivatives, resorcinol, cycloalkanone, methylenedioxyphenyl alkanol, 2,7-dinitroindazole or Plant extracts such as tangerine vine extract, rice extract, licorice extract, etc. are included, but are not limited thereto.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. can be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, and sarcosinate , fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention may further contain excipients including fluorescent substances, fungicides, hydrotropes inducing substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like. .

In addition, the present invention provides a health functional food composition for skin whitening and antioxidant containing fermented white paper extract as an active ingredient.

The composition may be prepared in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

When using the health functional food composition of the present invention as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. Active ingredients can be appropriately used depending on their intended use. In general, when preparing food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the type of health functional food. Examples of foods to which the health functional food composition can be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea There are drinks, alcoholic beverages, and vitamin complexes, and includes all health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be made into food, particularly functional food. The functional food of the present invention may contain ingredients that are commonly added. Examples include proteins, carbohydrates, fats, nutrients and seasonings. For example, when prepared as a drink, natural carbohydrates or flavors may be included as additional ingredients in addition to active ingredients. The natural carbohydrates are monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg, maltose, sucrose, etc.) , xylitol, sorbitol, erythritol, etc.) are preferred. As the flavoring agent, natural flavoring agents (eg, thaumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonic acid It may further contain a carbonation agent used in beverages and the like. The ratio of the components to be added is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

In addition, the present invention provides a pharmaceutical composition for preventing or treating hypermelanin hyperpigmentation disease containing a fermented product of white paper extract as an active ingredient.

As used herein, the term “melanin hyperpigmentation” means that certain parts of the skin or nails become black or darker than other parts due to an excessive increase in melanin. The melanin hyperpigmentation diseases include freckles, senile spots, liver spots, melasma, brown or dark spots, solar pigment spots, cyanic melasma, hyperpigmentation after drug use; post-inflammatory hyperpigmentation due to scratches, burns or dermatitis; or gestational brown spots (gravidic chloasma), etc., but are not limited thereto.

The pharmaceutical composition of the present invention may include pharmaceutically acceptable carriers in addition to active ingredients, and such carriers are commonly used in preparation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, Calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and minerals. oils and the like, but are not limited thereto. The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components.

A suitable dosage of the pharmaceutical composition according to the present invention is variously prescribed depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate and response sensitivity. It can be.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered by topical application to the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc. Considering that the pharmaceutical composition of the present invention is applied for the treatment or prevention of melanin hyperpigmentation disease, it is preferably applied topically to the skin.

The concentration of the active ingredient included in the composition of the present invention can be determined in consideration of the purpose of treatment, the condition of the patient, the required period, etc., and is not limited to a specific range of concentration.

The pharmaceutical composition of the present invention is prepared in unit dosage form by formulating it using a pharmaceutically acceptable carrier or excipient according to a method that can be easily performed by those skilled in the art, or It can be prepared by incorporating into a dose container. At this time, the formulation may be prepared as any one formulation selected from injections, creams, patches, sprays, ointments, warnings, lotions, liniments, pastas, and cataplasmas, and may additionally include a dispersing agent or a stabilizer. there is.

Hereinafter, the present invention will be described in more detail using examples. These examples are only for explaining the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention is not limited thereto.

Materials and Methods

1. Preparation of fermented product of white paper hot water extract and search for its optimal conditions

1) Checking the extraction yield of white paper extract according to the extraction solvent

White paper ethanol extract was prepared by adding 6 g of white paper to 100 mL of 70% ethanol, followed by extraction with stirring at room temperature for 6 hours.

White paper hot water extract was prepared by adding 6 g of white paper to 100 mL of distilled water, followed by extraction with stirring at room temperature for 6 hours.

As a result of comparative analysis by measuring the yield of white paper extract according to the extraction method (ethanol and hot water) of white paper, as shown in FIG. 1, it was confirmed that the yield of white paper hot water extract was significantly higher than that of white paper ethanol extract.

2) Exploration of optimal conditions for producing fermented products of baekji hot water extract

In order to prepare a fermented product using the white paper hot water extract using the ericium erinaceus mycelium, 1 to 20 parts by weight of white paper hot water extract were added to 100 parts by weight of distilled water, respectively, and 1 part by weight of glucose and 1 part by weight of sucrose were added. After adding, sterilized at 121 ° C. for 15 minutes and cooled, 10 parts by weight of Hericium erinaceus mycelium was inoculated with respect to 100 parts by weight of the sterilized solution.

In order to confirm the optimal conditions for fermenting the white paper hot water extract using the ericium erinaceus mycelium, the ratio of the white paper hot water extract and the temperature conditions were adjusted and cultured at 120 rpm for 25 days to prepare a fermented product of white paper hot water extract As a result, as shown in Table 1 below, the mycelial density was the highest at 23 ° C on the 15th day of culture, and the optimum temperature for fermentation of the white paper hot water extract was determined to be 23 ° C. In addition, as a result of fermentation using ericium erinaceus mycelium according to the content of the white paper hot water extract, fermentation efficiency was the best when 5 parts by weight of white paper hot water extract was added to 100 parts by weight of distilled water.

Therefore, based on 100 parts by weight of distilled water, 5 parts by weight of white paper hot water extract was added, 1 part by weight of glucose and 1 part by weight of sucrose were added, sterilized at 121 ° C. for 15 minutes and cooled, and then 10 parts by weight based on 100 parts by weight of the sterilized solution The fermented product of the white paper hot water extract fermented at 23 ° C. for 25 days after inoculation with ericium erinaceus mycelium was used as a sample for the following experiments.

condition Mycelial density (mg/15 days) Temperature (℃)/ White paper extract 5% (v/v) 17 213.4±10.8 18 226.7±8.7 19 236.4±9.7 20 241.7±9.2 21 248.4±11.1 22 271.6±8.9 23 288.3±7.2 24 269.3±6.4 25 256.2±8.8 26 239.4±11.2 White paper extract content with respect to 100 parts by weight of distilled water
(parts by weight)/23°C One 241.4±6.7 5 278.3±9.1 10 257.4±6.2 20 225.3±11.5

2. Cell viability measurement

B16F10 mouse melanoma cells were cultured, and 1 × 10 ⁵ cells/ml per well were dispensed per well in a 96-well plate, cultured, and stabilized for 24 hours. After treating the fermented white paper hot water extract and reacting for 24 hours, 10 μl of MTT (3-(4,5-dimethyldiazol-2-yl)-2,5-defenterazolium promid) reagent was added to Formazan was formed for 4 hours. After the reaction was completed, the medium was removed, and the formazan was dissolved with dimethyl sulfoxide (DMSO), and the absorbance was measured at 540 nm using an ELISA reader, and the result was calculated as a ratio of the value of the untreated control group.

3. Measurement of antioxidant activity

As a representative experiment comparing antioxidant activity, dark purple DPPH (α,α-diphenyl-β-picrylhydrazyl) is a relatively stable free radical, which is a substance that reduces aromatic compounds and amines through the electron donating ability of antioxidants. 0.1 ml of fermentation product (10 mg/ml) of white paper extract was added to 3.9 ml of 0.05 mM DPPH solution dissolved in 99.9% methyl alcohol, mixed for 10 seconds, and reacted at room temperature for 30 minutes while blocking light. After that, absorbance was measured at 517 nm. As a negative control, the same amount of distilled water as the fermented white paper extract was used, and as a positive control, vitamin C (1 mg/ml), widely known as an antioxidant, was used. DPPH scavenging ability was expressed as the concentration (IC ₅₀ ) of the sample showing a 50% decrease in absorbance relative to the control group.

4. Inhibitory activity of mushroom tyrosinase

Tyrosinase is an enzyme involved in the synthesis of melanin, which generates DOPA from L-tyrosine in the body and converts it to L-Dopaquinone again by causing continuous enzymatic oxidation. It causes freckles and melasma.

Measurement of the inhibitory activity of mushroom tyrosinase was performed by adding 0.2 ml of mushroom tyrosinase (200 U) to a mixture of 0.2 ml of 10 mM L-DOPA substrate solution and 0.1 ml of sample solution in 0.5 ml of 0.175 M phosphate buffer (pH 6.8). /ml) was added, mixed for 10 seconds, and reacted at 25° C. for 2 minutes, and then the absorbance of DOPA Chrome produced was measured at 475 nm. Mushroom tyrosinase inhibition rate (%) It was calculated in the following way.

[Equation 1]

Tyrosinase inhibition rate (%) = (absorbance of sample added/absorbance of non-added) × 100

Example 1. Confirmation of cell viability of fermented product of white paper hot water extract fermented using ericium erinaceus mycelium of the present invention

In Example 1, B16F10 cells were treated with the fermented product of the white paper hot-water extract fermented using 10, 50, 100, and 500 μg/ml of the mycelium of the ericium erinaceus of the present invention and the white paper hot-water extract, respectively, and reacted for 24 hours. After that, cell viability was measured.

As a result, as shown in FIG. 2, when the hot water extract of white paper at 10 to 100 μg/ml and the fermented product were treated, no cytotoxicity was observed. However, when the fermented product of 500 μg/ml hot-water extract of Baekji was treated, almost no cytotoxicity was observed. It was confirmed that fermentation can significantly reduce cytotoxicity occurring at high concentrations.

Example 2. Confirmation of antioxidant activity of fermented product of white paper hot water extract fermented using ericium erinaceus mycelium of the present invention

Antioxidant activities of the fermented white paper hot water extract fermented using the ericium erinaceus mycelium of the present invention and the white paper hot water extract were measured and analyzed for comparison.

As a result, as shown in FIG. 3, compared to the white paper ethanol extract, the white paper hot water extract had better antioxidant activity, and the white paper hot water extract had a significantly lower IC ₅₀ value than the white paper hot water extract, and the antioxidant activity was superior. could confirm that

Example 3. Confirmation of inhibitory activity of mushroom tyrosinase according to treatment of fermented product of baekji hot-water extract fermented using ericium erinaceus mycelium of the present invention

The inhibitory activity of mushroom tyrosinase of white paper hot water extract and white paper hot water extract fermented using the ericium erinaceus mycelium of the present invention was measured.

As a result, as shown in FIG. 4, not only the activity inhibitory effect of tyrosinase was better at all concentrations treated with the fermented product of the white paper hot water extract of the present invention compared to the white paper hot water extract, but in particular, 500 μg/day It was confirmed that the activity of tyrosinase was inhibited by about 54% when the fermented product of the white paper hot water extract of ㎖ was treated.

Example 4. Manufacturing and stability evaluation of a prototype to which the fermentation product of the white paper hot water extract fermented using the ericium erinaceus mycelium of the present invention was added

Toners and lotions containing the fermented product of the white paper hot water extract of the present invention were prepared, and their stability was evaluated.

1) pH stability

The pH of cosmetics was measured using a pH meter from Corning (pH meter440, Corning, USA), and the pH of toners and lotions added with fermented white paper hot water extract stored at 25 ° C was measured. Before the measurement, the glass electrode was calibrated with buffers of pH 4 and 7 in advance, and the detection part was washed with distilled water and lightly wiped before use, and stored for 30 days before measurement.

As a result of measuring the pH for 1 day, 3 days, 5 days, 7 days, 15 days and 30 days while storing the prototype product to which the fermented product of the white paper hot water extract was added at 25 ° C., as shown in FIG. 5, the toner had a pH 5.77 ± 0.03, lotion pH 6.34 ± 0.03, it was confirmed that there was no significant change over time.

2) Viscosity measurement

Viscosity of cosmetics was measured using a Brookfield viscometer (Brookfield LV, Brookfield Engineering Laboratories, Inc., USA). The spindle (spindle) was measured for 30 seconds at 6rpm by selecting No.64, and defoamed during manufacture to reduce the effect of air bubbles. The viscosity of the toner and lotion to which the fermented product of the white paper hot water extract stored at 25 ° C was added was measured. The results were obtained by repeating three times, and the values measured after being stored for 30 days are shown.

As a result, the toner showed 15,000 to 18,000 mPa and the lotion showed 26,000 to 28,000 mPa·s on average. As a result of measuring the viscosity over time, there was no significant change.

3) Stability measurement according to temperature

The stability test method according to temperature was stored at 5 ° C, 25 ° C, and 40 ° C, and the state change of toner and lotion according to the change over time was evaluated under the condition. When evaluating stability according to temperature, coagulation, precipitation, ballistics, separation, etc. were observed at low temperature (5 ° C), and rancidity, discoloration, odor change, viscosity, precipitation, turbidity, etc. were observed at high temperature (40 ° C).

As a result, the toner and lotion to which the fermentation product of the white paper hot water extract was added showed no significant change for 30 days, confirming the stability according to temperature.

4) Stability measurement according to freeze-thaw cycling

In the stability test method according to the cold and thaw cycle, the toner and lotion were put in a transparent container and stored at -10 ° C and 25 ° C for 24 hours, respectively. . As a result, both the toner and the lotion to which the fermented product of the white paper hot water extract was added were stable, and stable without phase separation in both the 3rd and 7th cycles.

Claims (8)

A cosmetic composition for skin whitening and antioxidant containing, as an active ingredient, a fermented product of an extract of white paper ( Angelica dahurica ) obtained by fermenting an extract of white paper using ericium erinaceus mycelium. delete The cosmetic composition for skin whitening and antioxidant according to claim 1, wherein the extraction solvent of the white paper extract is water, C ₁ ~ C ₄ lower alcohol or a mixture thereof. The method of claim 1, wherein the fermented product of the white paper extract
1) Adding 1 to 30 parts by weight of white paper hot water extract and 0.5 to 2.5 parts by weight of a carbon source based on 100 parts by weight of water and sterilizing at 110 to 130 ° C. for 10 to 20 minutes; and
2) Inoculate 5 to 15 parts by weight of ericium erinaceus mycelium with respect to 100 parts by weight of the sterilized solution of step 1) and then incubate at 15 to 30 ° C for 10 to 30 days; Characterized in a cosmetic composition for skin whitening and antioxidant. The cosmetic composition for skin whitening and antioxidant according to claim 1, wherein the fermented product of the white paper extract has less cytotoxicity compared to the white paper extract. A health functional food composition for skin whitening and antioxidant containing a fermented white paper extract obtained by fermenting white paper extract using ericium erinaceus mycelium as an active ingredient. A pharmaceutical composition for the prevention or treatment of hypermelanin hyperpigmentation disease containing, as an active ingredient, a fermented white paper extract obtained by fermenting a white paper extract using ericium erinaceus mycelium. The method of claim 7, wherein the melanin hyperpigmentation disease is freckles, senile spots, liver spots, melasma, brown or dark spots, solar pigment spots, cyanic melasma, hyperpigmentation after drug use; post-inflammatory hyperpigmentation due to scratches, burns or dermatitis; Or a pharmaceutical composition for the prevention or treatment of melanin hyperpigmentation disease characterized in that the pregnancy brown spots (gravidic chloasma).

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