KR102354412B1 - Cosmetic Composition Comprising Lactobacillus Fermented product of Mixed Flower Extracts as Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a lactic acid bacteria fermented product of a mixed flower extract as an active ingredient, and more particularly, it contains a fermented product obtained by fermenting a mixed extract of ginseng flower, panicle flower, jacaranda and glycolysis with lactic acid bacteria as an active ingredient. It relates to a cosmetic composition that
The cosmetic composition has a skin wrinkle improvement and antioxidant effect, thereby securing high value as a cosmetic.

Description

Cosmetic Composition Comprising Lactobacillus Fermented product of Mixed Flower Extracts as Active Ingredient

The present invention relates to a cosmetic composition capable of improving wrinkles and having antioxidant effects by containing a fermented product obtained by fermenting a mixed flower extract with lactic acid bacteria as an active ingredient.

Aging of the skin is caused by oxidative damage to biomaterials by free radicals. In vivo, oxidative or free radical attack and antioxidant defense are closely coordinated. However, antioxidant defense is not perfect, and biomolecules such as lipids, proteins, nucleic acids, and enzymes, which are bio-constituting molecules, continue to receive oxidative damage by oxidizing agents or free radicals, and these oxidation products accumulate with age.

In particular, the skin is always in contact with oxygen, and oxidative stress caused by exposure to UV rays is applied to the skin, thereby breaking down the enzymatic and non-enzymatic antioxidant barrier, resulting in lipid peroxidation, protein oxidation, and chain severing and abnormal crossing of collagen and elastin, which are elastic fibers. Damage to biological components such as binding, hyaluronic acid chain cleavage, melanin formation reaction promotion, and DNA oxidation occurs, eventually leading to skin aging in the form of reduced elasticity, pigmentation, and wrinkle formation. Therefore, antioxidants can be said to be an important factor in preventing skin aging.

Accordingly, studies on physiologically active substances having antioxidant activity from natural products are being actively conducted. As natural antioxidants, alpha-tocopherol, vitamin C, flavonoids, etc. are known. These antioxidants are widely distributed in animals and plants, and in particular, a lot of research is conducted on plant-derived substances.

Most of the natural antioxidants reported to date are derived from plants and are known to be mainly polyphenol compounds. In particular, flavonoids play a role in lipid oxidation, scavenging of free radicals and oxidative stress, thereby preventing aging, cancer and heart disease. Because it has the effect of preventing or delaying diseases, it is used in many fields such as cosmetics and pharmaceuticals.

For example, Republic of Korea Patent Registration No. 10-1452770 is an antioxidant containing lactic acid bacteria fermented product obtained by fermenting a mixed extract of pomegranate flower, pomegranate root, hollyhock, plum root, plum flower, plum tree root, lotus and lotus root with lactic acid bacteria as an active ingredient. , discloses a cosmetic composition having a whitening and wrinkle improvement effect.

KR 10-1452770 R

As a result of various efforts to produce a cosmetic composition having antioxidant and anti-wrinkle effects using natural materials as raw materials, the present inventors selected a specific flower and fermented the mixed extract thereof at low temperature using lactic acid bacteria. The present invention was completed by finding out that it has an antioxidant and anti-wrinkle effect and has a useful effect on skin improvement.

Accordingly, an object of the present invention is to provide a cosmetic composition having antioxidant and anti-wrinkle effects.

In order to achieve the above object, the present invention provides a cosmetic composition containing, as an active ingredient, a fermented product obtained by fermenting a mixed flower extract of ginseng flower, panicle flower, jacaranda flower, and hyacinth flower as an active ingredient.

In addition, the present invention comprises the steps of obtaining a mixed flower extract by extracting ginseng flower, panicle flower, jacaranda flower and corresponding flower flower with water;

It provides the fermented product manufacturing method comprising the step of inoculating the mixed flower extract with lactic acid bacteria and then fermenting to obtain a lactic acid bacteria fermented product.

The cosmetic composition comprising the lactic acid bacteria fermented product of the mixed flower extract of the present invention as an active ingredient has anti-wrinkle and antioxidant effects, thereby securing high value as a cosmetic.

1 is a graph showing the results of comparing the free radical scavenging effect of the fermented product of Example 1 and the extract of Comparative Example 1.
Figure 2 is a graph showing the results of comparing the antioxidant effect of the fermented product of Example 1 and the extract of Comparative Example 1.
3 is a graph showing the result of comparing the tropoelastin expression improvement effect of the fermented product of Example 1 and the extract of Comparative Example 1.

Hereinafter, the present invention will be described in more detail.

The present invention provides a cosmetic composition containing, as an active ingredient, a lactic acid bacteria fermented product of a mixed flower extract having anti-wrinkle and antioxidant effects, and using the mixed flower extract as a raw material for the cosmetic composition, wrinkle improvement and antioxidant by low-temperature fermentation Maximize the effect.

As the mixed flowers, gold hydrangea, paniculata, jacaranda and glycoliflower can be used.

Inula britannica flower is a dicotyledonous perennial plant in the order Asteraceae, commonly grown in fields and at the foot of mountains. It is a wild plant distributed in East Asia including Korea, Japan, and China. The components of the flower of Geumbulchoi include patuletin 7-O-(6''-isobutyryl)glucoside, patuletin 7-O-[6'' -(2-methylbutyryl)]glucoside (patuletin 7-O-[6''-(2-methylbutyryl)]glucoside), patuletin 7-O-(6''-isovaleryl)-glucoside It contains flavonoids such as 7-O-(6''-isovaleryl)-glucoside), patulitrin, patuletin, and luteolin.

There are several types of conifers, but they are mainly H. fulva var . kwanso Regel, H. middendorfii Trautv. et Meyer, H. dumortieri Morr, and H. thunbergii Bak. is used for food and medicinal purposes.

Wonchuri is called Nammul in Korean and Geumchimchae (金針采) in Chinese. In spring, young shoots are picked, and in summer, flowers and buds are picked and eaten with kimchi or seasoned with vegetables. Wonchuri is a mild and light wild vegetable with a sweet, soft, and smooth taste. The young shoots are picked and lightly blanched in red pepper paste, or the young shoots are used raw as a soup, or fried, or fried in oil to make a delicacy. When rice is cooked after removing the tufts from the flowers, the rice will turn yellow and give off a unique scent.

Campis grandiflora k. Schum is a deciduous tendril of the Jacaraceae family, reaching 10 m in length, and the stem is yellowish-brown. The flower is relatively large with a diameter of 4-5 cm, and the calyx is 3 cm long, divided into 5 parts, and blooms in August-September. In oriental medicine, flowers that have just begun to bloom are dried in the sun and used as a live blood medicine and as an analgesic for postpartum diseases, menstrual irregularities, diuretics, and antipyretics.

Rosa rugosa THUNB is a deciduous shrub belonging to the Rosaaceae family that is commonly grown on the seashores and foothills of East Asia. The flowers are also called maegaehwa and pilduhwa, and have been used in oriental medicine and folk medicine for the treatment of hematemesis, menstrual irregularities, and dysentery.

In the present invention, the above-mentioned Geumbulcho flower, panicle flower, jacaranda flower, and jacaranda flower are used as a raw material for a cosmetic composition in the form of an extract, and at this time, each plant for preparing the extract is ginseng flower, panicle flower, jacaranda flower, and jacaranda flower. It is used in a weight ratio of 1:1:1:1~3:3:3:1. This content ratio is obtained in consideration of the effect of each component, particularly the antioxidant effect and skin wrinkle improvement effect. If the content is less than the above range, the efficacy is insignificant. As a result of the by-products, problems such as sedimentation or providing a cause of irritation occur.

In particular, in the present invention, the fermented product of the mixed flower extract including Geumbulcho flower, panicle flower, jacaranda flower, and glycosylated flower is obtained through a low-temperature fermentation process after a conventional extraction process.

Specifically, the mixed flower extract fermented product according to the present invention is

A step of obtaining a mixed flower extract by extracting ginseng flower, panicle flower, jacaranda flower and corresponding flower flower with water;

Obtaining a lactic acid bacteria fermented product by inoculating the mixed flower extract with lactic acid bacteria and then fermenting

manufactured through

First, a mixed flower extract is prepared by extracting ginseng flower, panicle flower, jacaranda flower and corresponding flower flower with water.

Geumbongcho flower, primrose flower, jacaranda flower, and lily of the valley used as raw materials are purchased from the market or directly harvested from the wild or wild.

As the extraction solvent that can be used, one selected from the group consisting of water, a polyhydric alcohol having 1 to 6 carbon atoms, a lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, and a mixed solvent thereof is used, and preferably water 2 to 50 times (volume) of the weight of the raw material is added.

For the extraction, conventional methods such as hot water extraction, room temperature extraction, high pressure extraction, warming extraction, ultrasonic extraction or supercritical extraction may be used.

As the extraction temperature, a person skilled in the art may adopt various temperature ranges suitable for the extraction method, for example, it may be carried out at 20 °C to 100 °C, but is not limited thereto. In addition, the extraction time is different depending on the extraction method, and a person skilled in the art may adopt an appropriate extraction time, but is not limited thereto, but may be performed single or multiple times in the range of about 1 hour to several days. The extract obtained by performing extraction with the primary extraction solvent is obtained in a liquid form from which impurities are removed by filtration according to a conventional method, or the obtained liquid extract is concentrated and/or dried under reduced pressure according to a conventional method to obtain a powder form can be obtained

Next, the mixed flower extract is inoculated with lactic acid bacteria and then fermented to obtain a lactic acid bacteria fermentation product.

The fermentation refers to a process of decomposing organic matter using the efficacy of microorganisms. Fermentation and decay proceed by a similar process, but when a substance useful in our daily life is produced as a result of decomposition, it is called fermentation. Therefore, when herbal medicines are fermented using microorganisms, the toxicity of herbal medicines is reduced, the molecular weight is reduced to facilitate digestion, or when applied to the skin, transdermal absorption is facilitated, thereby improving bioavailability. In addition, although natural ingredients can exhibit pharmacological action by themselves, the pharmacological effect can be further strengthened through fermentation or can exert a pharmacological action that was not present in traditional herbal medicines prior to fermentation by the metabolism of microorganisms.

In the case of fermenting the mixed flower extract according to the present invention, the microorganisms used are not limited, but it is preferable to inoculate Lactobacillus sakei as a lactic acid bacterium.

At this time, in the fermentation process, the mixed flower extract is inoculated with lactic acid bacteria (concentration of 10 ⁵ to 10 ⁸ cfu/ml), and cultured for 6 to 60 days while maintaining the conditions of pH 4-8 and temperature 0-10 ℃. In particular, by performing the fermentation process at a low temperature, the fermentation period is prolonged, but the content of physiologically active substances is increased, so that excellent antioxidant efficacy can be expected.

The fermented product thus obtained After centrifugation, it is filtered. This is by separating the fermented product through centrifugation, removing insoluble substances such as cells and recovering the supernatant, performing paper filtration of 0.3 to 0.5 μm, preferably 0.45 μm, followed by sterilization filtration to prepare a fermented product according to the present invention do.

The fermented product is preferably contained in an amount of 0.1 to 90% by weight in the total cosmetic composition according to the present invention. This is because, when the content of the fermented product is less than 0.1% by weight, the effect of skin improvement cannot be expected, and even if the content exceeds 90% by weight, there is no significant improvement in effectiveness, and the stability of the formulation is deteriorated.

The composition according to the present invention may contain, in addition to the above active ingredients, other ingredients capable of giving a synergistic effect to the main effect, preferably within a range that does not impair the main effect aimed at the present invention.

In addition, the ingredients included in the cosmetic composition of the present invention contain ingredients commonly used in cosmetic compositions in addition to the above active ingredients, for example, conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers. can do.

The composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, emulsion, gel, lotion, essence, cream, powder, soap, face mask, cleansing agent, oil, It may be formulated as a cosmetic such as foundation.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, aluminum metabolite Hydroxide, microcrystalline cellulose, bentonite, agar or tracanth and the like can be used.

When the formulation of the present invention is a cream or gel, wax, paraffin, tracanth, animal oil, starch, cellulose derivatives, silicone, bentonite, polyethylene glycol, silica, zinc oxide or talc may be used as a carrier component. When the formulation of the present invention is a powder or spray, it may contain a propellant such as silica, talc, aluminum hydroxy group, lactose, calcium silicate, chlorofluorohydrocarbon, propane/butane or dimethyl ether as a carrier component.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, isethionate, methyl taurate, sarcosinate, fatty acid amide ether as carrier components Sulfate, aliphatic alcohol, alkylamidobetaine, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used.

Hereinafter, preferred examples are presented to help the understanding of the present invention. However, the following examples are only provided for easier understanding of the present invention, and the content of the present invention is not limited by the examples.

Example One: mixed flower lactic acid bacteria in the extract fermented product Produce

Geumbulchoflower, primrose flower, jacaranda flower, and corresponding flower flower harvested by season were prepared either as they are or after steaming and drying. Mixed flower extract by adding water corresponding to 20 times the weight of the raw material mixed with the above-mentioned ginseng flower, panicle flower, jacaranda flower and corresponding flower flower in a weight ratio of 1:1:1:1 and extracting it at high pressure (1.5 atmospheres). got

The Lactobacillus sakei strain cultured in the resulting mixed flower extract was inoculated at 10 ⁷ cfu/ml cfu/g, sealed, and fermented at 8°C for 7 days.

After sterilization at high temperature and high pressure (12l°C, 1.5 atm), 0.45㎛ paper filtration was performed to remove the raw material (solid phase) to prepare a fermented product.

comparative example One : mixed flower extract preparation

The mixed flower extract of Example 1 It was prepared by removing the raw material (solid phase) by filtration of 0.45㎛ paper filtration.

Experimental example One: free radical (Free Radical) Efficacy Test

Antioxidant activity was measured using the DPPH method using the antioxidant as a comparative sample.

The DPPH method uses a free radical called 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) to measure antioxidant activity by reducing power. By comparison, the free radical scavenging rate is measured at a wavelength of 560 nm. As a reagent used, 61.88 mg as a 0.1 Mm solution of 2,2-diphenyl-1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 394.32 g/mol) was dissolved in methanol to make 100 ml. The specific measurement method is as follows. .

1. To a 96-well plate, add 0.15 ml of a sample solution dissolved in 0.1 (wt/v)% and 0.5 (wt/v)% purified water to 0.15ml of 0.1mM DPPH solution, respectively, and stir quickly at 25°C for 10 minutes culture was started.

2. Thereafter, the absorbance St at 560 nm was measured. 3. For the blank test, use distilled water instead of the sample solution and measure the absorbance Bt in the same manner as above.

4. Again, the blank of the sample solution was operated in the same manner using methanol instead of 0.1mM DPPH solution, and absorbance Bo was measured.

The result of the effect was calculated by Equation 1 below, and the result is shown in FIG. 1 .

Referring to FIG. 1 , it was found that the lactic acid bacteria fermented product of the mixed flower extract of Example 1 had a significantly higher free radical scavenging effect than the mixed flower extract of Comparative Example 1.

Experimental example 2: xanthine oxidase extinction ability Confirm

Xanthine oxidase generates urea and superoxide radicals from xanthine. It is known that a large portion of superoxide generated in vivo is caused by this enzyme. The scavenging activity of superoxide, which causes various diseases due to cell destruction, was analyzed using NBT (nitro-blue tetrazolium) reduction method. That is, after mixing 0.1 mL of sample with 1 mL of 0.1 M potassium phosphate buffer (pH 7.5) containing 0.4 mM xanthine and 0.24 mM NBT and 0.6 mL of 0.1 M potassium phosphate buffer (pH 7.5), 1.0 mL of xanthine oxidation After adding the enzyme (0.05 U/mL) and incubating at 37°C for 20 minutes, 1.0 mL of 1M HCl was added to terminate the reaction, and then absorbance was measured at 520 nm. The inhibitory ability for NBT reduction according to the concentration samples was calculated by Equation 2 below, and the results are shown in FIG. 2 .

Referring to FIG. 2 , the fermented product of Example 1 had higher antioxidant activity than the mixed flower extract of Comparative Example 1 and exhibited higher antioxidant activity than vitamin C.

Experimental example 3: Confirmation of the effect of increasing the expression of elasticity-related factors

^{Fibroblasts (CCD-986Sk) were adjusted to 5×10 5} cells/well using IMDM medium supplemented with 10% FBS, and then inoculated in a 12-well plate and cultured for 18 hours. After culturing, the samples of Example 1 and Comparative Example 1 were treated and cultured for 24 hours. RNA was extracted from the cultured cells, and the expression increase rate of tropoelastin was confirmed through RT-PCR (Reverse transcription polymerase chain reaction). Tropoelastin expression increase rate was calculated by the following formula, the result is shown in FIG. As a control, adenosine was used.

As shown in FIG. 3 , it can be seen that the mixed flower extract lactic acid bacteria fermented product of the present invention has a superior tropoelastin expression improvement effect than the mixed flower extract.

Hereinafter, a cosmetic composition containing a mixed flower extract lactic acid bacteria fermented product is formulated and presented based on the above experimental examples, but the formulation of the present invention is not necessarily limited thereto.

Preparation Example 1. Skin

As shown in Table 1 below, a skin containing the mixed flower extract lactic acid bacteria ferment of Example 1 was prepared according to a conventional method.

ingredient content (wt%) Purified water 77.08 Mixed Flower Extract Lactobacillus Fermented Product 10.00 glycerin 3.00 dipropylene glycol 2.00 PEG-400 0.30 panthenol 0.50 Disodium EDIT 0.02 Sodium Hyaluronate 0.05 1,2-Hexanediol 1.50 PEG-60 Hydrogenated Castor Oil 0.50 ethanol 5.00 incense 0.05

Preparation Example 2. Lotion

As shown in Table 2 below, a lotion containing the mixed flower extract lactic acid bacteria ferment of Example 1 was prepared according to a conventional method.

ingredient content (wt%) Purified water 66.53 Mixed Flower Extract Lactobacillus Fermented Product 10.00 glycerin 5.00 Butylene Glycol 5.00 PEG-400 0.30 panthenol 0.50 Disodium EDIT 0.02 Carbomer 0.30 Sodium Hyaluronate 0.05 1,2-Hexanediol 2.00 ceramide 0.10 Glycerin Stearate 2.80 Sorbitan Stearate 1.20 cetearyl stearate 1.70 Stearic Acid 1.00 beeswax 0.80 triethylhexanoin 3.00 squalane 3.00 Cyclomethicone 1.50 dimethicone 0.50 triethanolamine 0.30 incense 0.10

Preparation Example 4. Essence

As shown in Table 3 below, an essence containing the mixed flower extract lactic acid bacteria ferment of Example 1 was prepared according to a conventional method.

ingredient content (wt%) Mixed Flower Extract Lactobacillus Fermented Product 10.00 glycerin 3.00 dipropylene glycol 5.00 PEG-400 0.30 panthenol 0.50 Disodium EDIT 0.02 Sodium Hyaluronate 0.05 Acrylate/C10-30 alkyl acrylate crosspolymer 0.10 1,2-Hexanediol 1.50 ceramide 0.50 triethanolamine 0.10 incense 0.05

Preparation 5. Mask Pack

As shown in Table 4 below, a mask pack containing the mixed flower extract lactic acid bacteria ferment of Example 1 was prepared according to a conventional method.

ingredient content (wt%) Purified water 66.53 Mixed Flower Extract Lactobacillus Fermented Product 10.00 glycerin 5.00 Butylene Glycol 5.00 PEG-400 1.50 panthenol 0.50 Disodium EDIT 0.02 Carbomer 0.30 xanthan gum 0.10 Sodium Hyaluronate 0.05 1,2-Hexanediol 2.00 cetearyl alcohol 1.50 Glycerin Stearate 2.80 Sorbitan Stearate 1.20 cetearyl stearate 1.70 Stearic Acid 0.50 beeswax 0.80 triethylhexanoin 3.00 squalane 3.00 Cyclomethicone 3.00 dimethicone 5.00 triethanolamine 0.30 incense 0.10

Claims (10)

Geumbulcho flowers, daylily flowers, flower jacaranda and sweetbrier contain the fermented water into effect the mixing of a flower extract Lactobacillus four K (Lactobacillus sakei) as an active ingredient, a cosmetic composition for improving expression of troponin elastin. The cosmetic composition according to claim 1, wherein the fermented product is contained in an amount of 0.1 to 90% by weight based on the total weight of the composition. delete The cosmetic composition according to claim 1, wherein the fermented product has an antioxidant effect. The cosmetic composition according to claim 1, wherein the fermented product has an effect of improving skin wrinkles. The method according to any one of claims 1, 2, 4 and 5, wherein the cosmetic composition is a solution, suspension, emulsion, gel, lotion, essence, cream, powder, soap, face mask, cleansing, A cosmetic composition having one formulation selected from the group consisting of oil, foundation and spray. A step of obtaining a mixed flower extract by extracting ginseng flower, panicle flower, jacaranda flower and corresponding flower flower with water;
Step of inoculating the mixed flower extract with Lactobacillus sakei and then fermenting it to obtain a lactic acid fermented product
The method of claim 1 comprising a fermented product. delete The method according to claim 7, wherein the fermented product is obtained by filtration after sterilization. The method according to claim 7, wherein the fermentation is performed at 0 to 10 °C for 6 to 60 days.

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