KR102131008B1 - Composition for enhancing skin elasticity or improving skin wrinkle comprising fermentated extract of inula britannica flower - Google Patents

Abstract

The present invention relates to a composition for improving skin elasticity or improving skin wrinkles, including a fermented sunbok extract.

Description

Composition for improving skin elasticity or improving skin wrinkles including fermented bokhwahwa extract {COMPOSITION FOR ENHANCING SKIN ELASTICITY OR IMPROVING SKIN WRINKLE COMPRISING FERMENTATED EXTRACT OF INULA BRITANNICA FLOWER}

The present invention relates to a composition for improving skin elasticity or improving skin wrinkles, including a fermented sunbok extract.

In modern society, waste products such as peroxides accumulate in the human body due to changes in diet, lack of exercise, and heavy mental stress in the relationship with a more complex society. Induces inflammation or decreases immunity and may also cause cancer.

That is, peroxide is easily generated in unsaturated fatty acids, particularly among lipids present in the human body, and is promoted by various factors. In the case of food, it is automatically derived from oxygen, heating, ultraviolet light, mechanical shock, the presence of metal ions, etc., but in the human body, it is also produced by lipid oxidase such as lipooxygenase. These substances are very unstable and highly reactive, attacking and damaging the DNA in the cell, and attacking the cell membrane, which is the main component of lipids, causing chain fatty acidation to promote aging of skin tissue. Therefore, research on wrinkle improvement may be conducted in relation to skin aging.

Therefore, research on antioxidants has been actively conducted recently, and especially polyphenols as natural materials are stabilized as a resonance structure by absorbing free radicals into the phenol ring molecule itself, and at the same time forming free radicals. It is known to inhibit various chain oxidation by. Polyphenols are very diverse depending on the type of plant, and a typical example is a flavonoid. Flavonoids have C ₆ -C ₃ -C ₆ as their main skeleton and exist in nature as a glycoside associated with one or more sugars. Flavonoids are mainly present in the fruit or flower parts of plants, and in particular, the color of the fruit or flower is also cleared. Flavonoids are classified according to their structure, and according to the location of functional groups, flavanols, flavanones, flavones, isoflavones, flavonols, anthocyanidins It is divided into small groups. In addition to strong antioxidant power, physiological functions have been studied to have antibacterial and dementia prevention, anti-inflammatory and anti-cancer effects, and these functions are known to be somewhat different depending on the small group of flavonoids.

As an example, a cosmetic composition for anti-inflammatory and anti-oxidation of green tea and pine mushroom extract has been filed, and Kim et al. studied antioxidant and anti-inflammatory activity of the grated wormwood fraction. In particular, polyphenols, among other antioxidants, are known to have the strongest oxidizing power of flavonoids, and many studies have been conducted recently due to their additional physiological functions.

Collagen is a high-molecular protein present in muscles, and especially in subcutaneous muscles, its content decreases with age. In particular, collagenase (collagenase) acts as an important factor along with skin aging by degrading collagen, reducing skin elasticity and causing skin wrinkles. Currently, substances having an inhibitory effect of collagenase on skin and muscle are actively being studied using natural materials as one of skin elasticity enhancement and skin wrinkle improvement.

However, bio-active substances in natural products do not reach actual effective concentrations, and various processing methods such as biological conversion through extraction, concentration, or fermentation are required industrially. That is, most active ingredients present in natural products exist as glycosides or are combined with other components. Therefore, in order to increase the extraction yield, various solvents are used depending on the polarity or enzymatic extraction by microorganisms. However, in practice, only ethanol is permitted in the use of a solvent in food production, so there is a limitation in selecting a solvent. However, in the case of the fermentation method, it is possible to efficiently extract and extract only the active ingredient from the glycosides using enzymes produced by microorganisms, and further, to proceed with biological conversion, higher physiological functionality can be expected. For example, a patent has been filed to further highlight functionality through fermentation using Aspergillus fungus.

The present invention is to provide a pharmaceutical composition for improving skin elasticity or improving skin wrinkles, including fermented bokhwahwa extract.

However, the technical problem to be achieved by the present invention is not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The present invention provides a pharmaceutical composition for improving skin elasticity or improving skin wrinkles, including fermented sunbok extract.

The fermentation may be by Lactobacillus plantarum strain KCCM 11613P.

The bokbok extract may be extracted with a solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, lower ketones having 1 to 4 carbon atoms, lower esters having 1 to 4 carbon atoms, chloroform, and mixtures thereof. have.

The number of lactic acid bacteria live cells in the fermented bokbokhwa extract may be 7.0 Log cfu/mL or more.

In the fermented sunbok extract, pH may be 4.5 or less.

The fermented bokbok extract is compared to a non-fermented bokbok extract, i) DPPH radical scavenging ability; Ii) ABTS radical scavenging ability; Iv) superoxide anion scavenging ability; Or iii) the reducing power for iron trivalent ions or copper divalent ions may be improved.

The concentration of the fermented sunbok extract may be 10 μg/ml to 10 mg/ml.

In one embodiment of the present invention, it provides a health functional food for improving skin elasticity or improving skin wrinkles including fermented sunbok extract.

In another embodiment of the present invention, a cosmetic composition for improving skin elasticity or improving skin wrinkles including a fermented sunbok extract is provided.

The composition for enhancing skin elasticity or improving skin wrinkles according to the present invention is characterized by comprising a fermented sunbok extract, wherein the fermented sunbok extract has an inhibitory effect of collagenase, which improves skin elasticity Alternatively, it may be useful for improving skin wrinkles.

In addition, the fermented sunbok extract is compared to the non-fermented sunbok extract, iv) DPPH radical scavenging ability; Ii) ABTS radical scavenging ability; Iv) superoxide anion scavenging ability; Or iii) an antioxidant effect, such as an improvement in reducing power for iron trivalent ions or copper divalent ions, is improved.

1 is a graph comparing the DPPH radical scavenging ability of fermented bokbok extract (□: Example) and non-fermented bokbokbok extract (■: Comparative Example).
Figure 2 is a graph comparing the ABTS radical scavenging ability of the fermented sunbok extract (□: Example) and the non-fermented sunbok extract (■: Comparative Example).
Figure 3 is a graph comparing the superoxide anion scavenging ability of the fermented sunbok extract (□: Example) and the non-fermented sunbok extract (■: Comparative Example).
Figure 4 is a graph comparing the reducing power for the iron trivalent ions of the fermented bokbok extract (□: Example) and non-fermented bokbokbok extract (■: Comparative Example).
Figure 5 is a graph comparing the reducing power for copper divalent ions of fermented bokbok extract (□: Example) and non-fermented bokbokbok extract (■: Comparative Example).
Figure 6 is the inhibitory effect of the collagenase (collagenase) of the non-fermented bokbokhwa extract (□: Comparative Example), and the inhibitory effect of collagenase (collagenase) by concentration of fermented bokbokhwa extract (■: Example) It is a graph comparing.

The present inventors can fertilize the vinegar used as a medicinal plant using lactic acid bacteria, thereby increasing the concentration of an active ingredient having an inhibitory effect of collagenase, thereby improving skin elasticity or improving skin wrinkles. It confirmed, and completed this invention.

The present invention provides a pharmaceutical composition for improving skin elasticity or improving skin wrinkles, including fermented sunbok extract.

In this specification, “skin elasticity” and “skin wrinkles” are well-known symptoms of skin aging, and collagenase (collagenase) is used to improve skin elasticity or improve skin wrinkles for the purpose of preventing skin aging. ) It is necessary to inhibit the activity.

Sunbokhwa (scientific name Inula britannica ), which is used in the pharmaceutical composition for improving skin elasticity or improving skin wrinkles according to the present invention, is a wild flower that is native to East Asia, such as Korea, China, and Japan. Sunbokhwa has been used for the treatment of digestive disorders, bronchitis, and the like since ancient times. Particularly, the ablation of flavonoids such as quercetin, kaempferol, and luteolin is abundant in pre-enhancement, and essential oils have various types of sesquiterpene lactone as well as antioxidant activity. ) To induce apoptosis of various cancer cells and thereby kill cancer cells. Therefore, these phytochemicals are absorbed into the human body and exhibit various functions, thereby providing functionality for health promotion.

In general, flavonoids in natural products mostly exist as glycosides, and the flavonoids in glycoside form have lower efficiency than aglycone form, and various processing methods such as extraction, concentration, or fermentation are required to reach an effective concentration for actual functionality.

Accordingly, the pharmaceutical composition for improving skin elasticity or improving skin wrinkles according to the present invention includes fermented bokbok extract, that is, bokbok water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, and 1 to 4 carbon atoms. After extraction with a solvent selected from the group consisting of lower ketones, lower esters of 1 to 4 carbon atoms, chloroform and mixtures thereof, after inoculation of the lactic acid bacteria to the extracted bokhwa, fermented bokhwa extract fermentation.

The bokbok extract is a solvent selected from the group consisting of dried bokbok water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, lower ketone having 1 to 4 carbon atoms, lower ester having 1 to 4 carbon atoms, chloroform, and mixtures thereof. It may be extracted, and it is preferable that the dried sunbok is extracted with 500 mL to 2000 mL of water or ethanol based on 100 g of the sunbok sun dried, with reference to the solvent announced by the Ministry of Food and Drug Safety, but is limited to this. Does not work.

At this time, the extraction may be performed at a high temperature, it is preferably extracted for 12 hours to 48 hours at a temperature of 50 ℃ to 70 ℃, but is not limited thereto.

The fermented bokbok extract refers to the bokbok extract fermentation broth. When using such fermentation, it is possible to efficiently extract and extract only active ingredients from glycosides using enzymes produced by lactic acid bacteria, and further proceed to biological conversion. If you do, you can expect higher physiological functionality. In other words, the effective substance of the pre-enhancement has a problem in that its concentration is low in nature, and thus it is difficult to apply industrially. However, by enhancing the effective substance in the fermentation broth using lactic acid bacteria that are harmless to the human body, its functionality is increased and especially improved collagenase. ) Can provide industrial applicability due to its inhibitory effect.

The fermentation may be by lactic acid bacteria, preferably by lactic acid bacteria isolated from kimchi, more preferably by Lactobacillus plantarum KCCM 11613P.

The Lactobacillus plantarum is a representative probiotics, and is a gram-positive bacterium, non-spore forming bacteria in the form of bacilli. In particular, Lactobacillus plantarum KCCM 11613P is a strain isolated from kimchi and has high acid resistance and bile resistance, resulting in a survival rate of 99% and 97.9%, respectively, showing higher values than other lactobacillus plantarums . It is also a strain that has an anti-allergic effect.

Specifically, the lactobacillus is preferably inoculated in 0.1 to 10 parts by volume with respect to 100 parts by volume of the pre-enriched extract, but is not limited thereto in terms of optimal fermentation conditions and thus antibacterial activity.

At this time, the fermentation is preferably performed for 20 hours to 100 hours at 20 ℃ to 40 ℃, but is not limited thereto.

The number of lactic acid bacteria live cells in the fermented bokbokhwa extract may be 7.0 Log cfu/mL or more. In addition, in the fermented bokhwahwa extract, the pH may be 4.5 or less, preferably the pH may be 4.0 or less. This change in pH can be considered to be attributable to organic acids produced by the growth of lactic acid bacteria.

The fermented bokbok extract is compared to a non-fermented bokbok extract, i) DPPH radical scavenging ability; Ii) ABTS radical scavenging ability; Iv) superoxide anion scavenging ability; Or iii) the reducing power for iron trivalent ions or copper divalent ions is improved. As a result, the antioxidant effect may be improved.

The concentration of the fermented sunbok extract is preferably 10 μg/ml to 10 mg/ml, but is not limited thereto. At this time, when the concentration of the fermented sunbok extract is less than the above range, the effect of inhibiting collagenase activity is negligible, and thus there is a problem that it is difficult to properly exhibit effective skin elasticity improvement or skin wrinkle improvement effect, and fermented sunbokization If the concentration of the extract exceeds the above range, there may be a concern about toxicity, including cytotoxicity.

Each of the pharmaceutical compositions may be formulated and used in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injectable solutions, according to a conventional method. It may include suitable carriers, excipients or diluents commonly used in the manufacture of pharmaceutical compositions for the purpose of chemical conversion.

Examples of the carrier or excipient or diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, and crude Various compounds or mixtures including vaginal cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When formulated, it can be prepared using diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents, surfactants, etc., which are usually used.

Solid preparations for oral administration may be prepared by mixing at least one excipient, for example, starch, calcium carbonate, sucrose or lactose, gelatin, etc., with the fermented sunbok extract. In addition, lubricants such as magnesium stearate and talc may be used in addition to simple excipients.

Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are common diluents, various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, can be included. .

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous agents, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin butter, and glycerol gelatin can be used.

The preferred dosage of the pharmaceutical composition varies depending on the patient's condition, weight, disease severity, drug form, administration route, and duration, but may be appropriately selected by those skilled in the art. However, for a desired effect, it can be administered at 0.0001 to 2,000 mg/kg per day, preferably at 0.001 to 2,000 mg/kg per day. Administration may be administered once a day, or may be divided into several times. However, the scope of the present invention is not limited by the dosage.

The pharmaceutical composition may be administered to various mammals, such as rats, mice, livestock, and humans. All modes of administration can be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrathecal dura or intracerebroventricular injection.

In addition, the present invention provides a health functional food for improving skin elasticity or improving skin wrinkles, including fermented sunbokhwa extract.

The health functional food for improving skin elasticity or improving skin wrinkles according to the present invention includes fermented bokbok extract, since the above-mentioned fermented bokbok extract is described above, a duplicate description will be omitted.

In the health functional food composition, when the fermented sunbok extract is used as an additive of a health functional food, it can be added as it is or used with other foods or food ingredients, and can be suitably used according to a conventional method. The mixing amount of the active ingredient can be appropriately determined according to each purpose of use, such as prevention, health, or treatment.

The formulation of the health functional food composition may be in the form of powders, granules, pills, tablets, capsules, as well as general food or beverages.

The type of the food is not particularly limited, and examples of foods to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, and ice cream. , Various soups, beverages, teas, drinks, alcoholic beverages and vitamin complexes, and may include all foods in the ordinary sense.

In general, when preparing food or beverage, the fermented sunbok extract can be added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on 100 parts by weight of the raw material. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of health control, the amount may be below the above range, and the present invention does not have a problem in terms of safety in terms of using natural substances, so the amount above the above range Can also be used as

The beverage in the health functional food composition may contain various flavoring agents or natural carbohydrates, etc., as additional components, like a normal beverage. The natural carbohydrates described above may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as taumatin and stevia extract, synthetic sweeteners such as saccharin and aspartame can be used. The ratio of the natural carbohydrate may be about 0.01 ~ 0.04 g per 100 mL of the beverage, preferably about 0.02 ~ 0.03 g.

In addition to the above, the health functional food composition includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols , It may contain a carbonation agent used in carbonated drinks. In addition, the health functional food composition may contain flesh for the preparation of natural fruit juice, fruit juice beverage and vegetable beverage. These ingredients can be used independently or in combination. The ratio of these additives is not limited, but is generally selected from 0.01 to 0.1 parts by weight compared to 100 parts by weight of the health functional food of the present invention.

In addition, the present invention provides a cosmetic composition for improving skin elasticity or improving skin wrinkles, including fermented bokbokhwa extract.

The cosmetic composition for improving skin elasticity or improving skin wrinkles according to the present invention includes a fermented sunbok extract, which has been described above, and thus, duplicate description will be omitted.

The cosmetic composition includes, but is not limited to, a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

The water-soluble vitamin may be any compound that can be blended into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. And salts (thiamine hydrochloride, sodium ascorbate salt) or derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.), but are not limited thereto.

The oil-soluble vitamin may be any compound that can be blended into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and derivatives thereof (Ascorbyl palmitate, ascorbyl stearate, ascorbyl dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) It includes, but is not limited to.

The polymer peptide may be anything as long as it can be blended into cosmetics, but collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin or keratin is preferred, but is not limited thereto.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics, but hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate, or salts thereof (such as sodium salt) are preferred, but are not limited thereto.

The sphingo lipid may be any compound as long as it can be blended into cosmetics, but ceramide, phytosphingosine, and sphingoglycolipids are preferred, but are not limited thereto.

The seaweed extract may be anything as long as it can be blended into cosmetics, but brown seaweed extract, red seaweed extract or green algae extract is preferred, and colorazine, arginic acid, sodium arginate, or potassium arginate purified from these seaweed extracts are preferred. It is not limited to this.

In addition to the essential ingredients, other ingredients that are usually blended into the cosmetic composition may be added to the cosmetic composition. Examples of the other ingredients include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavors, blood circulation promoters, Cooling agents, limiting agents or purified water is preferred, but is not limited thereto.

As the fat or oil component, ester-based fats and oils, hydrocarbon-based fats and oils, silicone-based fats and oils, animal fats or vegetable fats are preferred, but not limited thereto. Examples of the ester-based fats and oils include glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid. Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacinate, adipine Diisopropyl acid, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, tri2-ethylhexanoate trimethylolpropane, triisostearate trimethylolpropane, tetra2-ethylhexanoate pentaerythritol , Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, isostea laurate Lyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl oleate, isopropyl isostearate, and isotosterate 2-ethylhexanoate Aryl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di(capryl, capric acid)propylene glycol, propylene glycol dicaprylic acid propylene glycol, dica Neopentyl glycol phthalate, neopentyl glycol dioctanoate, glyceryl tricaprylic acid, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl tetrapentanoate, isostearyl octanoate , Octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diacid malate Isostearyl, 2-ethylhexyl hydroxystearate, 2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, Cholesteryl stearate, Cholesteryl isostearate, Cholesteryl hydroxystearate, Cholesteryl oleate, Dihydrocholesteryl oleate, Pitsteryl isostearate, Pitsteryl oleate, 12-Stealoyl Esteryl hydroxystearate, 12-stealoylhydroxystearate stearyl, or 12-stealoylhydroxystearate isostearyl is preferred, but is not limited thereto.

As the hydrocarbon-based fat and oil, hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, or waselin are preferred, but are not limited thereto. .

Examples of the silicone oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxanemethylcetyloxysiloxane copolymer, dimethylsiloxanemethylstealoxysiloxane copolymer, and alkyl modification. Silicone oil or amino-modified silicone oil is preferred, but is not limited thereto.

The fluorine-based fat and oil is preferably a perfluoropolyether, but is not limited thereto.

Examples of the animal or plant oil include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapes Seed Oil, Cottonseed Oil, Palm Oil, Cucumin Nut Oil, Wheat Germ Oil, Rice Germ Oil, Shea Butter, Moon Seed Colostrum, Marker Demi Nut Oil, Meadow Home Oil, Yolk Yolk Oil, Uji, Horse Oil, Mink Oil, Orange Rape Oil, Jojoba Animal or vegetable oils, such as milk, candeler wax, carnauba wax, liquid lanolin or hardened castor oil are preferred, but are not limited thereto.

The moisturizing agent is preferably a water-soluble low molecular moisturizing agent, a fat-soluble molecular moisturizing agent, a water-soluble polymer or a fat-soluble polymer, but is not limited thereto. As a water-soluble low-molecular moisturizing agent, serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n=2 or more), polyglycerin B (polymerization degree n=2 or more), lactic acid or lactic acid salt is preferred, but is not limited thereto. Cholesterol or cholesterol ester is preferred as a fat-soluble low molecular humectant, but is not limited thereto. As the water-soluble polymer, carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan or dextrin are preferred. , But is not limited to this. As the oil-soluble polymer, polyvinylpyrrolidone ecocene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, or polymer silicone is preferred, but is not limited thereto. As the emollient agent, long-chain acyl glutamate cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid or lanolin fatty acid cholesteryl ester is preferred, but is not limited thereto.

The surfactant is preferably a nonionic surfactant, anionic surfactant, cationic surfactant, or amphoteric surfactant, but is not limited thereto. As the nonionic surfactant, self-emulsifying monostearic acid glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE Glycerine

Dissipative Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POEPOP (Polyoxyethylene Polyoxypropylene) Copolymer, POEPOP Alkyl Ether, Polyether Modified Silicone, Al laurol Acid Lauricamide, Alkylamine Oxide or hydrogenated soybean phospholipids are preferred, but not limited to. Examples of the anionic surfactant include fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, and alkylamide Phosphate, alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate salt, alkyl sulfosuccinate salt, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt or perfluoroalkyl phosphate ester is preferred, It is not limited to this. Examples of the cationic surfactant include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, and chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropylamide stearate, or a quaternary ammonium salt of a lanolin derivative is preferred, but is not limited thereto. Examples of the amphoteric surfactant include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type or amideamine type. Positive surfactants are preferred, but are not limited thereto.

Examples of the organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and oxidation. Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzenestyrene copolymer, silk Organic pigments such as powder, cellulose, CI pigment yellow, and CI pigment orange, and composite pigments of these inorganic and organic pigments are preferred, but are not limited thereto.

Examples of the organic powder include metal soaps such as calcium stearate; Alkyl phosphate metal salts such as sodium cetyl acid, zinc lauryl acid, and calcium lauryl acid; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-, such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylazine, N-alphaparitoylolnitine, N-alpha-lauroylarginine, N-alpha-cured Uji fatty acid acyl arginine Acyl basic amino acids; N-acylpolypeptides, such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-amino caprylic acid and alpha-amino lauric acid; Or polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzenestyrene copolymer, ethylene tetrafluoride is preferred, but is not limited thereto.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate, cinnamic acid Benzyl, paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, uro Canonic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone disulfonate sodium, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p -(carbo-2'-ethylhexyl-1'-oxy)-1 ,3,5-triazine or 2-(2-hydroxy-5-methylphenyl)benzotriazole is preferred, but is not limited thereto.

Examples of the fungicide include hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zincfilionone, benzalkonium chloride, Photoresist No. 301, mononitroguaercol sodium or undeciric acid is preferred, but is not limited thereto.

The antioxidant is preferably butyl hydroxyanisole, propyl gallic acid, or elisoric acid, but is not limited thereto.

Examples of the pH adjusting agent include, but are not limited to, citric acid, sodium citrate, malic acid, sodium malate, phthalic acid, sodium phthalate, succinic acid, sodium succinate, sodium hydroxide or sodium monohydrogen phosphate.

The alcohol is preferably a higher alcohol such as cetyl alcohol, but is not limited thereto.

In addition, the compounding components that may be added in addition are not limited thereto, and any of the above components can be blended within a range not impairing the objects and effects of the present invention, but those blended at a weight percentage of 0.01 to 5% based on the total weight Preferably, it is more preferably blended in a 0.01 to 3% weight percentage, but is not limited thereto.

The cosmetic composition may take the form of a solution, emulsion, or viscous mixture, but is not limited thereto.

The components included in the cosmetic composition may include components commonly used in cosmetic compositions in addition to the compound as an active ingredient, and may include conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments, and fragrances. However, it is not limited thereto.

The cosmetic composition may be prepared in any formulation conventionally manufactured in the art, and may be prepared as an emulsion, cream, lotion, pack, foundation, lotion, cosmetic liquid or hair cosmetic material, but is not limited thereto. Specifically, the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, Soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, or body cleanser formulations.

When the formulation is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as carrier components. It is not limited to this.

When the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane/butane Or it may include a propellant such as dimethyl ether, but is not limited thereto.

When the formulation is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, and water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3- Fatty acid esters of butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan can be used, but are not limited thereto.

When the formulation is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspensions such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline cellulose, Aluminum metahydroxide, bentonite, agar or trakant may be used, but is not limited thereto.

When the formulation is surfactant-containing cleansing, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate , Alkylamidobetaine, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives or ethoxylated glycerol fatty acid esters, but are not limited thereto.

As described above, the composition for improving skin elasticity or improving skin wrinkles according to the present invention is characterized by comprising a fermented sunbok extract, wherein the fermented sunbok extract has an inhibitory effect of collagenase. Bar, it can be useful for improving skin elasticity or improving skin wrinkles.

In addition, the fermented sunbok extract is compared to the non-fermented sunbok extract, iv) DPPH radical scavenging ability; Ii) ABTS radical scavenging ability; Iv) superoxide anion scavenging ability; Or iii) an antioxidant effect, such as an improvement in reducing power for iron trivalent ions or copper divalent ions, is improved.

Hereinafter, preferred embodiments are provided to help understanding of the present invention. However, the following examples are only provided to more easily understand the present invention, and the contents of the present invention are not limited by the following examples.

< Example >

Example

(One) Sunbokhwa Hydrothermal Preparation of extract

The pre-incubation sample was dried for 10 hours while maintaining a relative humidity of 4-5% at 60 using an incubator (Jeio-Tech Co., Seoul, Korea). The dried pears were ground to a size of 10 mm or less using a high-speed mixer. 50 g of crushed sunbok was mixed with 500 mL of water and extracted for 60 to 24 hours. The pre-heated hot water extract was freeze-dried until use and stored at -20.

(2) Fermented Sunbokhwa Hydrothermal Preparation of extract

Lactobacillus plantarum KCCM 11613P, a useful lactic acid bacterium isolated from kimchi, was used for fermentation of pre-entrained hot water extract and liquid fermentation was performed. After adding 500 mL of pre-entrained hot water extract to a 1 L Erlenmeyer flask and 1% (w/v) peptone to supplement the growth source required for the growth of microorganisms, titrate the pH to 6.5 using 0.1 N NaOH and then 121 1.5 The medium for fermentation was prepared by sterilization at atmospheric pressure for 15 minutes. Lactobacillus plantarum KCCM 11613P incubated 3 times at 12-hour intervals in the finished medium was inoculated with about 5 Log CFU/mL and fermented while shaking and cultured at a rate of 30 and 50 rpm for 72 hours. After fermentation, the remaining cells were removed using a 0.45 ?m membrane filter and freeze-dried and stored at -20. This was dissolved in 70% ethanol, and again, an insoluble substance was removed using a 0.45 ?m membrane filter, and then used in experiments described below.

At this time, Lactobacillus plantarum KCCM 11613P was deposited with the Korea Microbiological Conservation Center on November 27, 2014, and was given an accession number KCCM 11613P.

Comparative example

A non-fermented pre-fermented hot water extract was prepared in the same manner as in Example, except that the fermentation of the pre-enriched hot water extract was omitted.

Experimental Example 1: pH, lactic acid bacteria Live bacteria And yield measurement

The fermented pre-entrained hot water extract of the Example and the non-fermented pre-entrained hot water extract of the comparative example were measured for pH, lactic acid bacteria viable count and yield, respectively.

Specifically, the pH was measured using a pH meter (Model 720, WTW Co., Germany), and the number of lactic acid bacteria was directly measured using a standard plate culture method by plating the fermentation broth on an MRS solid medium, and the yield was measured in a dry state. The weight change was measured as a reference. The results are shown in Table 1 below.

Example Comparative example pH 3.98±0.01 4.73±0.02 Lactobacillus viable cell count
(Log CFU/mL) 7.4±0.1 - Yield (%) 12.4±1.1 15.2±0.1

As shown in Table 1, the pH of the fermented pre-entrained hot water extract of the Example was confirmed to decrease to about 3.98, while the number of lactic acid bacteria was increased to about 7.4 Log CFU/mL. In addition, the fermented pre-entrained hot water extract of the Example is confirmed to have a lower yield than the non-fermented pre-entrained hot water extract of the comparative example. It uses flavonoids in the form of glycosides during the growth of microorganisms during fermentation and converts them into separated glycocon, reducing the weight of the final fermented pre-entrained hot water extract by the reduced sugar weight. As a result, it can be seen that the yield was lowered.

Experimental Example 2: check the antioxidant effect

To confirm the antioxidant effect of the fermented pre-entrained hot water extract of the Example and the non-fermented pre-entrained hot water extract of the comparative example, DPPH radical scavenging capacity measurement, ABTS radical scavenging capacity measurement, superoxide anionic scavenging capacity measurement, and measurement of reducing power for iron trivalent ions And a reducing power measurement experiment for copper divalent ions.

(One) DPPH Radical Erasing ability Measure

The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability of the fermented pre-entrained hot water extract of the Example and the non-fermented pre-entrained hot water extract of the comparative example was measured using a DPPH radical scavenging assay.

Specifically, 1 mL of 100 μM DPPH solution dissolved in ethanol and a sample dissolved in distilled water (fermented pre-entrained hot water extract of Example or non-fermented pre-entrained hot-water extract of Comparative Example) were reacted in the dark for 15 minutes, 517 Absorbance was measured at nm. Samples were tested at concentrations of 62.5, 125, 250 and 500 μg/mL, DPPH radical scavenging capacity (%) was calculated as follows, and the results are shown in FIG. 1:

DPPH radical scavenging capacity (%) = (1 A _sample /A _control )× 100

In the above formula, A _control is the absorbance of the control and A _sample is the absorbance of the sample.

As shown in FIG. 1, the non-fermented pre-entrained hot water extract of the comparative example was found to have a DPPH radical scavenging ability of only 51.67% at a concentration of 500 μg/mL, whereas the fermented pre-entrained hot water extract of the example was DPPH at the same concentration. The radical scavenging activity was found to be 92.65%, indicating that the DPPH radical scavenging activity increased by about 79% due to fermentation.

(2) ABTS Radical Erasing ability Measure

The ABTS radical scavenging ability of the fermented pre-entrained hot water extract of Example and the non-fermented pre-entrained hot water extract of Comparative Example is 2, 2′-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS) radical scavenging fish It was measured using a sey.

Specifically, 2 mM ABTS and 2.45 mM potassium persulphate were reacted in distilled water for 12 hours to generate ABTS radicals. Thereafter, the resulting ABTS radical was diluted to obtain an absorbance of 0.700 at 734 nm, which was used in the experiment. After reacting 1 mL of ABTS radical solution with 50 μL of sample in the dark for 30 minutes, absorbance was measured at 734 nm. The samples were tested at concentrations of 62.5, 125, 250, and 500 μg/mL, and the ABTS radical scavenging ability was calculated using a trolox standard curve and calculated as TEAC (Trolox Equivalent Antioxidant Capacity), and the results are shown in FIG. 2. .

As shown in FIG. 2, the non-fermented pre-entrained hot water extract of the comparative example was confirmed to show the radical scavenging ability of 477.17 μM/mL TEAC at a concentration of 500 μg/mL, whereas the fermented pre-entrained hot water extract of the Example has the same concentration. In 694.25 μM/mL It was confirmed that the radical scavenging ability of TEAC was shown, and it can be seen that the ABTS radical scavenging ability increased by about 45% due to fermentation.

(3) Superoxide Anion( Superoxide anion) Erasing ability Measure

NBT-NADH-PMS complex of nitroblue tetrazolium (NBT), nicotinamide adenine dinucleotide (NADH) and phenazine methosulphate (PMS) of superoxide anion scavenging ability of fermented pre-entrained hot water extract of Example and non-fermented pre-entrained hot water extract of Comparative Example It was measured using a superoxide anion scavenging assay that acts as an oxidant through formation and measures the inhibition of NBT activity.

Specifically, 78 μM NBT, 468 μM NADH, and 1 mL of each sample were added, followed by reaction for 5 minutes by adding 0.4 mL of 60 μM PMS. Thereafter, absorbance was measured at 560 nm. The samples were tested at concentrations of 62.5, 125, 250 and 500 μg/mL, and the superoxide anion scavenging capacity (%) was calculated as follows, and the results are shown in FIG. 3:

Superoxide anion scavenging ability (%) = (A _control A _sample )/A _control × 100

In the above formula, A _control is the absorbance of the control and A _sample is the absorbance of the sample.

As shown in FIG. 3, the non-fermented pre-entrained hot water extract of the comparative example was found to have a superoxide anion scavenging ability of only 84.37% at a concentration of 500 μg/mL, while the fermented pre-entrained hot water extract of the example was at the same concentration. As the superoxide anion scavenging ability was found to be 86.38%, it can be seen that the superoxide anionic scavenging ability increased due to fermentation, showing a significant difference ( p <0.05).

(4) Measurement of reducing power for iron trivalent ions

The reducing power for the iron trivalent ions of the fermented pre-entrained hot water extract of Example and the non-fermented pre-entrained hot water extract of Comparative Example was measured.

Specifically, the sample and 1% potassium ferricyanide were dissolved in 0.2M sodium phosphate buffer (pH 6.6) and reacted for 20 minutes at 50°C for 200 μL each. Thereafter, 200 μL of 10% trichloroacetic acid was added to acidify the reaction solution, and then 500 μL of 0.1% iron chloride (III) aqueous solution was added for reaction for 20 minutes to measure absorbance at 700 nm. When the sample reduces the iron trivalent ion to the iron divalent ion, the iron divalent ion reacts with the potassium ion to show a blue color. The samples were tested at concentrations of 62.5, 125, 250 and 500 μg/mL, and the results are shown in FIG. 4.

As shown in FIG. 4, the fermented pre-entrained hot water extract of the Example records a higher absorbance than the non-fermented pre-entrained hot water extract of the comparative example, and it is confirmed that the reducing power for iron trivalent ions is improved. In particular, when the concentration of the sample is 125 and 500 μg / mL, it can be seen that it shows a significant difference ( p <0.05).

(5) Measurement of reducing power for copper divalent ions

The reducing power for copper divalent ions of the fermented pre-entrained hot water extract of Example and the non-fermented pre-entrained hot water extract of Comparative Example was measured.

Specifically, 7.5 μm neocuporine ethanol solution, copper(II) chloride aqueous solution, and 200 μL of sample were added to 1200 μL of 1 M ammonium acetate buffer (pH 7.4) and reacted for 30 minutes. Thereafter, absorbance was measured at 450 nm. The samples were tested at concentrations of 62.5, 125, 250 and 500 μg/mL, and the results are shown in FIG. 5.

As shown in FIG. 5, the fermented pre-entrained hot water extract of the Example is compared with the non-fermented pre-entrained hot water extract of the comparative example, and as a result, it is confirmed that the reducing power for copper trivalent ions is improved. In the human body, enzymes that promote oxidation reactions, such as tyrosinase, are promoted by metal divalent ions such as copper divalent ions. Therefore, it is believed that the enhanced reducing power for copper divalent ions due to fermentation can inhibit the oxidative enzyme activity in the human body, thereby improving the antioxidant effect.

Experimental Example 3: Confirm the inhibitory effect of collagenase (collagenase)

In order to verify the effect of improving the skin elasticity and improving skin wrinkles of the fermented pre-entrained hot water extract of the Example, the inhibitory effect on this was confirmed using a collagenase derived from Clostridium bacteria.

Specifically, N -[3-(2-furyl)acryloyl]-Leu-Gly-Pro-Ala(FLAGPA) was used as a substrate, 100 μL 1.1 U/mL of collagenase, 300 μL sample, and 600 μL tricine buffer (0.05 M, pH 7.5) was mixed to induce enzyme activity at 37° C. for 20 minutes. Thereafter, 200 μL 1 mM FLAGPA was added to carry out the enzymatic reaction for 10 minutes, and then absorbance was confirmed at 350 nm, and the effect of inhibiting collagenase was confirmed through the residual amount of FLAGPA. It is shown in.

As shown in FIG. 6, the fermented pre-entrained hot water extract of the Example was found to have a higher collagenase inhibitory effect than the non-fermented pre-entrained hot water extract of the comparative example at the same concentration. In addition, it was confirmed that the fermented pre-entrained hot water extract of the Example has an increased inhibitory effect on collagenase as the concentration increases.

The above description of the present invention is for illustration only, and a person having ordinary knowledge in the technical field to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Korea Microbial Conservation Center (Overseas) KCCM11613P 20141127

Claims (9)

The fermented sunbok extract is included at a concentration of 125 µg/ml to 10 mg/ml,
The fermented sunbok extract is inoculated with Lactobacillus plantarum strain KCCM 11613P into the sunbok hot water extract, and then fermented at 20°C to 40°C for 20 hours to 100 hours.
Pharmaceutical composition for improving skin elasticity or improving skin wrinkles.
delete delete According to claim 1,
The number of lactic acid bacteria in the fermented pre-enriched extract is 7.0 Log cfu/mL or more
Pharmaceutical composition for improving skin elasticity or improving skin wrinkles.
According to claim 1,
In the fermented sunbok extract, pH is 4.5 or less
Pharmaceutical composition for improving skin elasticity or improving skin wrinkles.
According to claim 1,
The fermented bokbok extract is compared to the non-fermented bokbok extract,
Iv) DPPH radical scavenging ability;
Ii) ABTS radical scavenging ability;
Iv) superoxide anion scavenging ability; or
Iv) characterized by improved reducing power for iron trivalent or copper divalent ions.
Pharmaceutical composition for improving skin elasticity or improving skin wrinkles.
delete The fermented sunbok extract is included at a concentration of 125 µg/ml to 10 mg/ml,
The fermented sunbok extract is inoculated with Lactobacillus plantarum strain KCCM 11613P into the sunbok hot water extract, and then fermented at 20°C to 40°C for 20 hours to 100 hours.
Health functional food for skin elasticity improvement or skin wrinkle improvement.
The fermented sunbok extract is included at a concentration of 125 µg/ml to 10 mg/ml,
The fermented sunbok extract is inoculated with Lactobacillus plantarum strain KCCM 11613P into the sunbok hot water extract, and then fermented at 20°C to 40°C for 20 hours to 100 hours.
Cosmetic composition for enhancing skin elasticity or improving skin wrinkles.

Images