KR20170052811A - yeast fermented solutions of green tea extract and manufacturing method thereof and cosmetic composition using the same - Google Patents
yeast fermented solutions of green tea extract and manufacturing method thereof and cosmetic composition using the same Download PDFInfo
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- KR20170052811A KR20170052811A KR1020150154626A KR20150154626A KR20170052811A KR 20170052811 A KR20170052811 A KR 20170052811A KR 1020150154626 A KR1020150154626 A KR 1020150154626A KR 20150154626 A KR20150154626 A KR 20150154626A KR 20170052811 A KR20170052811 A KR 20170052811A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
Description
The present invention relates to a yeast fermentation broth of green tea extract, a preparation method thereof, and a cosmetic composition using the same, and more particularly, to a yeast fermentation broth which has greatly enhanced antioxidant ability by fermenting green tea extract with yeast, Which is high in antioxidant activity and can be prepared into a skin cosmetic composition on the skin, a process for producing the yeast fermentation broth, and a cosmetic composition using the same.
The skin is the largest organ of the body, always in direct contact with the external environment, and serves as a protective layer for protecting the living body from various stimuli or dry environment. It is an organ where new cells are generated and eliminated extensively as compared with other organs. In addition, it protects the body from physical abrasion, protects the inside of the body from moisture loss, protects it from ultraviolet rays generated from the sun, and plays a very important role in controlling body temperature.
These externalities, such as wrinkles, loss of elasticity, and keratinization, are caused by external stimuli due to various physical and external environmental factors. Skin aging is largely classified into natural aging (or endogenous aging) and external aging. Natural aging is difficult to artificially control because it is affected by genetic factors, whereas external aging is affected by environmental factors. Do.
Exemplary extrinsic aging factors include ultraviolet light and the most prominent external aging phenomenon is wrinkle formation (Daniell HW, Ann. Intern. Med., 1971, 75 (6), 873; Grove GL et al., J. Am. Acad Dermatol., 1989, 21 (3), 631; Griffiths CE et al., Arch. Dermatol., 1992, 128, 347). One of the photo-aging mechanisms caused by ultraviolet light is via free radical pathways (Harman D, J. Gerontol., 1956, 11 (3), 298).
In normal cells, free radicals and other active oxygen and peroxides are produced to some extent during metabolism. In the body, however, superoxide dismutase (SOD), catalase, And antioxidant enzymes such as peroxidase and antioxidants such as vitamin C, vitamin E, glutathione, ubiquinone and uric acid are present to protect themselves. However, oxidative stress is caused when an abnormality occurs in such a biological defense mechanism or when the production of active oxygen by various physical and chemical factors exceeds the capacity of a biological defense system. Therefore, suppression of active oxygen is an important factor in skin aging cosmetics.
Antioxidants such as tocopherol, polyphenols,
Since many plant species contain various polyphenols with excellent plant protection effect, many plant extracts such as green tea, mallow, and gold are widely used in cosmetics as antioxidants. Natural antioxidants known to date include tocopherols, flavonoids, gossypol, sesamol, oryzanol, and vitamin C, among others.
On the other hand, fermentation refers to the process of decomposing organic matter by an enzyme secreted by a microorganism. It is also fermentation that microorganisms produce useful substances in the process of obtaining energy. Traditional Korean fermented foods such as doenjang and cheonggukjang are typical examples of kimchi, as well as familiarity with Koreans. This fermentation process is an anaerobic respiration microorganism that completely decomposes organic matter and produces other kinds of organic matter, thus generating a small amount of energy. These kinds of fermentation are lactic acid fermentation, alcohol fermentation, propionic acid fermentation, and methane fermentation depending on microorganisms. Lactic acid bacteria, which are representative of microorganisms involved in such fermentation, decompose sugars such as glucose or lactose to produce organic acids such as lactic acid and acetic acid, and produce enzymes such as amylase, cellulase, lipase and protease as byproducts, Thereby facilitating skin penetration.
Recently, natural material extracts applied to cosmetics have applied fermentation technology as a method of maximizing their efficacy and effectiveness. Among them, the fermentation yeast fermentation method promotes penetration of the skin by lowering the nutrients and promotes the penetration of the skin. It is a technique to make various effects such as moisturizing and wrinkling at one time because of the high concentration of amino acids similar to the skin moisturizing factor , Development of new materials combining the existing herbal extract material with such enrichment and fermentation technology is underway.
The fermentation in the cosmetics removes the fermenting bacteria in the process of fermenting the microorganisms and simultaneously extracts the active ingredients other than the enzyme, thereby doubling the effect of the existing enzyme and rapidly absorbing the ingredients into the skin. In addition, amino acids, vitamins, and various functional materials are produced and synthesized, the destruction of nutrients hardly occurs, and the fermentation products are substances that are eaten and excreted by yeast, 8, No. 3, No. 286-287).
The natural cosmetics fermentation material market is mainly composed of fermented extract materials using EM beneficial microorganisms. Currently, research is focused on cosmetic compositions using lactic acid bacteria rather than yeast.
The present invention has been made in order to overcome the above problems, and it is an object of the present invention to provide a yeast fermentation broth having significantly enhanced antioxidative ability by fermenting green tea extract with yeast, establishing optimal fermentation conditions, Which has high antioxidant ability and provides a skin cosmetic composition to the skin.
To achieve the above object, the yeast fermentation broth of green tea extract of the present invention is characterized in that the green tea extract is fermented by yeast to increase the antioxidative activity of the green tea extract.
Wherein the yeast is Saccharomyces Levy MY access to three cyano (Saccharomyces cerevisiae .
According to another aspect of the present invention, there is provided a method for preparing a yeast fermentation broth of a green tea extract, comprising the steps of: extracting a green tea extract by adding an extraction solvent to a green tea; A fermentation step of adding yeast to the green tea extract to ferment; An inactivating step of inactivating the yeast by heat-treating the yeast fermentation broth obtained in the fermentation step; And filtering the supernatant obtained by centrifuging the yeast fermentation broth after the inactivation step to remove the residue.
The green tea is extracted with the extraction solvent at a weight ratio of 2 to 40 times at a temperature of 10 to 150 ° C for 1 to 20 hours, and centrifuged to obtain a supernatant. The supernatant is filtered under reduced pressure to remove the green tea extract .
The fermentation step is the green tea extract, yeast extract per 100mL 0.3g, 0.3g malt extract, peptone 0.5g, three to Levy cyano saccharide followed by the addition of glucose, 1g sterilized for 15 minutes in 121 ℃ My process (Saccaromyces cerevisiae to 2% (v / v) and fermented at 80 rpm at 34 DEG C for 72 hours to obtain the yeast fermentation broth.
To achieve the above object, the cosmetic composition of the present invention comprises 0.1 to 40% by weight of a yeast fermentation broth which fermented green tea extract as a yeast to increase the antioxidative activity of the green tea extract. The cosmetic composition contains lotion, cream, essence, The composition is formed of any one of a makeup base, a foundation, a body oil, a body lotion, a cleansing oil, a cleansing foam, a hair oil, a hair shampoo and a hair rinse.
According to the present invention, by fermenting green tea extract with yeast, the antioxidant ability can be greatly increased as compared with the green tea extract before fermentation.
Therefore, the cosmetic composition containing the yeast fermentation broth of green tea extract has excellent antioxidative activity and is hypodermic to the skin, thus providing a high-performance cosmetic composition.
Fig. 1 shows the results of measuring the radical scavenging activity of the green tea extract, the yeast fermentation broth of Example 1, and the yeast fermentation broth of the comparative example,
FIG. 2 shows the results of measuring the total polyphenol content of the green tea extract, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example,
FIG. 3 shows the results of measuring the total flavonoid content of the green tea extract, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example,
Fig. 4 is a summary of a skin test result report of the shampoo of Example 2 and the body cleanser of Example 3; Fig.
Hereinafter, a yeast fermentation broth of green tea extract according to a preferred embodiment of the present invention, a preparation method thereof, and a cosmetic composition using the same will be described in detail.
The yeast fermentation broth of green tea extract of the present invention is obtained by fermenting green tea extract with yeast. This yeast fermentation solution effectively increases the antioxidant ability of green tea extract.
A method for producing yeast fermentation broth of green tea extract comprises an extraction step of extracting green tea extract by adding an extracting solvent to green tea, a fermentation step of fermenting the green tea extract by adding yeast, an inactivating step of deactivating yeast by heating the yeast fermentation broth, , And a filtration step of filtering the supernatant obtained by centrifuging the yeast fermentation broth to remove the residue. Let's look at each step.
Extracts can be obtained by using commercially available extracts or by extracting them in various ways.
As an extract, extracts extracted from natural plants are used. Green tea extract can be used as such an extract. The green tea extract has been used for a long time as a material of a natural cosmetic composition and is thus proven to be safe for human body and has various physiological activities.
Green tea extract is obtained by adding extraction solvent to green tea. As the extraction solvent, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.
The green tea is mixed with the extraction solvent at a weight ratio of 2 to 40 times, and the mixture is then extracted with hot water, cold or hot extraction at 10 to 150 ° C for 1 to 20 hours. In addition to the above-described extraction method, an active fraction obtained through various purification methods such as a reflux cooling extraction method, an ultrasonic extraction method, and the like may be included.
The green tea extract is centrifuged to obtain a supernatant, and the supernatant is then filtered under reduced pressure to remove the solid residue.
In addition to the green tea extract, Sansago extract can be used.
Amana edulis ) is a perennial plant of lily lilies and grows in sunny grasses. The scales in the ground are oval-shaped and 3-4cm long. The stalks of the Sanjugo scales contain starch, which can act as an excipient. In the autumn or spring, the stalks are dried in the shade and dried naturally. The extraction method for obtaining the extract from Sanjiao can be applied in the same manner as the above-described green tea extract extraction method.
In addition, herbal medicine extracts may be used. Herbal medicine extracts further enhance functionality. One example of the medicinal herb may be ginseng, saururus chinensis, white radish sprout, seokchonpo mushroom, and hyphae lavender mixed at the same weight ratio. In addition, it can be added to the palace, creation, safflower, licorice, Angelica, allium, persimmon, windshield, serpentine, rhubarb, It can be extracted from hot water to obtain herbal medicine extracts from medicinal herbs. Extraction can be performed by known direct heat extraction, hot water extraction, etc. However, in order to effectively extract active ingredients of herbal medicines, it is preferable to extract by hot water extraction. The hot water extract is obtained by heating in a hot water bath at 100 to 120 ° C for 10 to 14 hours and cooling, and then removing insoluble solids such as foreign substances and debris from the filter. Herbal medicine extracts can be used alone or in combination with green tea extract. When mixed with the green tea extract, the green tea extract and the medicinal plant extract may be mixed at a weight ratio of 1: 0.2 to 0.6.
Next, yeast is added to the green tea extract and fermented. Saccharomyces cerevisiae ( Saccharomyces < RTI ID = 0.0 > cerevisiae ) can be used.
Yeast extract, Malt extract, Peptone, and Dextrose are added to the green tea extract during fermentation. For example, 0.3 g of yeast extract, 0.3 g of malt extract, 0.5 g of peptone, and 1 g of glucose may be added per 100 mL of green tea extract.
Yeast extract, malt extract, peptone, and glucose are added to the green tea extract and sterilized at 121 ° C for 15 minutes, followed by inoculation with yeast. Yeast is inoculated and fermented for 1 to 10 days while stirring at 50 to 100 rpm under a temperature condition of 20 to 40 캜 at a pH of 5 to 7 to obtain yeast fermentation broth.
Next, the obtained yeast fermentation broth is heat-treated to inactivate the yeast. For this, the yeast fermentation broth is heated to 50 ° C for 1 to 10 minutes.
Next, the inactivated yeast fermentation broth is centrifuged, and the supernatant is filtered under reduced pressure to remove the solid residue, thereby finally producing the filtered yeast fermentation broth.
The yeast fermentation broth can be prepared by adding various known components and the like to the cosmetic composition.
The cosmetic composition of the present invention may contain the yeast fermentation broth in an amount of 0.1 to 40.0% by weight based on the total weight of the composition. If the content is less than 0.1% by weight, the effect is insignificant. If the content is more than 40.0% by weight, the feeling of use may be deteriorated due to the unique odor generated during fermentation.
The cosmetic composition of the present invention may be prepared by adding various known components and the like to the cosmetic composition by using a method known in the art to prepare cosmetic lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, Hair oil, hair oil, hair shampoo and hair rinse.
Specifically, when the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel or a pack, known components include animal fibers, vegetable fibers, waxes, paraffin, starch, tragacanth, celluloses derivatives, polyethylene glycols, silicones, bentonites, Silica, talc or zinc oxide may be used.
When the formulation of the cosmetic composition of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or the like may be used.
When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or non-acidic esters of sorbitan, and the like.
When the formulation of the cosmetic composition of the present invention is a suspension, it may be diluted with water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Celluloses, aluminum metahydroxides, bentonites, agar or tragacanth, and the like can be used.
When the formulation of the cosmetic composition of the present invention is a surfactant-containing detergent, it is preferable to use a surfactant-containing detergent such as aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate , Alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils or ethoxylated glycerol fatty acid esters.
In addition, the cosmetic composition of the present invention may be prepared by any of the commonly known formulations such as stabilizers, solubilizers, vitamins, pigments, perfumes, and the like, which are conventionally produced in the art, And can be selected accordingly.
As an example of the formulation, the lotion was prepared by mixing 1.5 wt% of ethanol, 1.0 wt% of natural liquid, 3.0 wt% of betaine, 3.0 wt% of diel panthenol, 1.0 wt% of sebum-A, 2.0 wt% of arbutin paste, 2.0 wt% 0.03% by weight of glycerin, 4.0% by weight of glycerin, 1.0% by weight of collagen, 30.0% by weight of yeast fermentation broth, preservative (small amount) and purified water (balance).
In addition, as an example of the formulation, the lotion may contain 1.0% by weight of carbomer, 2.0% by weight of dimethicone, 5.0% by weight of jojoba oil, 0.5% by weight of provitamin E, 1.0% by weight of olive oil wax, 5.0% by weight of arbutin paste, 1.2 wt%, foam mix 1.0 wt%, batain 1.0 wt%, cetearyl alcohol 1.5 wt%, glycerin 4.0 wt%, flex oil 5.0 wt%, arbutin paste 5.0 wt%, yeast fermentation solution 30.0 wt% Oil (trace amount), preservative (trace amount) and purified water (residual amount).
As an example of the formulation, the cream may contain 1.5 wt% of cetearyl alcohol, 1.0 wt% of glyceryl stearate, 1.0 wt% of
The essence of the formulation is 1.5% by weight of ethanol, 1.0% by weight of natural liquid, 1.0% by weight of carbomer, 3.0% by weight of betaine, 2.0% by weight of adenosine, 5.0% by weight of arbutin paste, 0.03% by weight of diel panthenol, 4.0% by weight of glycerin, 1.0% by weight of sebum-A, 30.0% by weight of yeast fermentation broth, a blending essence oil, a preservative and a purified water.
As an example of the formulation, a foundation may be prepared by mixing 2.0 wt% of stearic acid, 0.5 wt% of cetearyl alcohol, 1.0 wt% of glyceryl stearate, 0.5 wt% of
In addition, as an example of the formulation, the pack may contain 0.5 wt% of propylene glycol, 1.0 wt% of natural liquid, 3.0 wt% of polyvinyl alcohol, 3.0 wt% of ethanol, 1.0 wt% of carbomer, 2.0 wt% of collagen, 1.0 wt% (By weight), 1.0 wt% of RF, 3.0 wt% of diel panthenol, 4.0 wt% of glycerin, 1.0 wt% of sebum-A, 2.0 wt% of adenosine, 30.0 wt% of yeast fermentation broth, ≪ / RTI >
As an example of the formulation, the hair shampoo may contain 0.5% by weight of polyquaternium, 1.0% by weight of natural liquid, 3.0% by weight of cocamidopropyl betaine, 3.0% by weight of betaine, 7.0% by weight of CDA, 25.0% by weight of LES, 5.0 weight% of collagen, 1.0 weight% of collagen, 3.0 weight% of diel panthenol, 4.0 weight% of glycerin, 1.0 weight% of sebum-A, 2.0 weight% of adenosine, 30.0 weight% of yeast fermentation broth, Purified water (remaining amount).
EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.
(Example 1)
1 L of distilled water was added to 40 g of green tea, heated at 70 ° C for 6 hours, and filtered to remove solids. Then, the supernatant was obtained by centrifugation at a speed of 6000 rpm for 20 minutes, and then the supernatant was filtered under reduced pressure to obtain a green tea extract from which the solid residue was removed.
Next, 0.3 g of yeast extract, 0.3 g of malt extract, 0.5 g of peptone, and 1 g of Dextrose were added to 100 mL of green tea extract, and sterilized at 121 ° C for 15 minutes. The yeast Saccaromyces cerevisiae (KCCM 50583) to 2% (v / v). The green tea extract inoculated with the yeast strain was fermented at 80 rpm for 72 hours while maintaining the temperature at 34 ° C. After the fermentation, the yeast strain was inactivated by heating at 50 ° C., followed by centrifugation twice at 12,000 rpm for 20 minutes. The resulting supernatant was filtered under reduced pressure to completely remove the solid residue to prepare yeast fermentation broth.
(Example 2)
The yeast fermentation broth of Example 1 was added to produce the following shampoo.
0.52 g of a polyquater as a thickening agent was added to 42 g of the yeast fermentation broth, and the mixture was first stirred with heating on a hot plate at 70 캜 until sufficient gelation occurred. After stirring, 40 g of an anionic surfactant LES, 6 g of a cationic surfactant cocobetaine, and 6 g of an amphoteric surfactant, CDA, were further mixed and stirred. Further, 0.8 g of glycerin as a humectant, 0.4 g of olive liquid as a solubilizing agent and 0.1 g of essence oil were added and mixed.
(Example 3)
The yeast fermentation broth of Example 1 was added to prepare a body cleanser.
To 42 g of yeast fermentation broth, 1.2 g of olive liquid as a solubilizing agent was metered in. After stirring, 40 g of an anionic surfactant LES, 6 g of a cationic surfactant cocobetaine, and 6 g of an amphoteric surfactant, CDA, were further mixed and stirred. 0.8 g of glycerin, 0.8 g of diel panthenol, 0.7 g of betaine and 0.2 g of essence oil were added as a moisturizing and functional material and mixed.
(Comparative Example)
To the 100 mL of distilled water, 0.3 g of yeast extract, 0.3 g of malt extract, 0.5 g of peptone and 1 g of Dextrose were added and sterilized at 121 ° C for 15 minutes. The yeast Saccaromyces cerevisiae (KCCM 50583) Respectively. The green tea extract inoculated with the yeast strain was fermented at 80 rpm for 72 hours while maintaining the temperature at 34 캜. After the fermentation, the yeast strain was inactivated by heating at 50 ° C., followed by centrifugation twice at 12,000 rpm for 20 minutes. The resulting supernatant was filtered under reduced pressure to completely remove the solid residue to prepare yeast fermentation broth.
≪ First Experimental Example: Measurement of radical scavenging ability &
The radical scavenging ability of the green tea extract obtained in Example 1, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example were measured by diluting the distilled water by the added concentration, respectively. The results are shown in FIG.
0.3 ml of the sample and 0.7 ml of 0.2 mM 1,1-diphenyl-2-picryl hydrazyl (DPPH) solution were mixed and left for 15 minutes in a dark room, and the absorbance was measured at 517 nm using a UV-vis spectrometer. The radical scavenging ability (%) was calculated by the following equation.
Radical scavenging activity (%) = (1-absorbance of sample added / absorbance of non-additive) x 100
Referring to FIG. 1, the radical scavenging ability of the comparative example was the lowest. And green tea extract showed higher radical scavenging ability than the comparative example. It was also found that the yeast fermentation broth of the green tea extract of Example 1 was greatly improved in radical scavenging ability compared to the green tea extract. From this, it was found that the fermentation of green tea extract with yeast could significantly enhance the antioxidant activity of green tea extract. Therefore, it is expected that the yeast fermentation broth of green tea extract is excellent in the antioxidative effect of the cosmetic composition when it is used as a material for a cosmetic composition.
≪ Second Experimental Example: Determination of total polyphenol content >
The green tea extract obtained in Example 1, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example were mixed with Folins-denis reagent at a ratio of 1: 1, 10% Na 2 CO 3 was added, Were measured. The total polyphenol content was measured based on tannic acid and is shown in FIG.
Referring to Figure 2, the total polyphenol content of the comparative example was the lowest. And green tea extract showed higher total polyphenol content than the comparative example. The yeast fermentation broth of green tea extract of Example 1 was found to have a greater total polyphenol content than green tea extract. These results are consistent with the results of the above-mentioned radical scavenging ability measurement.
≪ Third Experimental Example: Total flavonoid content of yeast fermentation filtrate >
The green tea extract obtained in Example 1, the yeast fermentation broth of Example 1, and the yeast fermentation broth of Comparative Example were mixed at a constant ratio of 1N NaOH and diethylene glycol, and left for 30 minutes, and the absorbance was measured at 420 nm. The total flavonoid content was measured using quercetin as a reference material and is shown in FIG.
Referring to FIG. 3, the total flavonoid content of the comparative example was the lowest. And green tea extract showed higher total flavonoid content than the comparative example. The yeast fermentation broth of the green tea extract of Example 1 was found to have a significantly higher total flavonoid content than the green tea extract. These results are consistent with the measurement results of the radical scavenging ability and the total polyphenol content.
≪ Fourth Experimental Example: Confirmation of irritancy of cosmetic composition >
To examine the degree of skin irritation of the shampoo of Example 2 and the body cleanser of Example 3, a skin test was commissioned by the Dermar Pro skin Science Institute. A summary of the skin test results report is attached in FIG.
A total of 31 participants participated in this study. The mean age of the subjects was 37.6 ± 8.5 years. The highest age group was 50 years old and the lowest age group was 20 years old.
The test area was washed with 70% ethanol and then dried. The test substance was diluted to 1% in distilled water due to the nature of the wash-off product. 16 μl of the test substance was dropped into the Finn chamber, placed on the back of the test site, and fixed with a micropore tape. After removing the patches, the test area was marked with a skin marker, and each test site was observed after 30 minutes and 24 hours.
Observations were made 30 minutes and 24 hours after removal of the patch, and skin reactions were evaluated according to the criteria reflecting the Frosch & Kligman1 method and the Personal Care Product Council (PCPC) guideline.
As a result of the test, 1 subject had a 1+ grade skin reaction in each of the two test substances. Therefore, the two test substances were identified as low-irritant substances in terms of primary skin irritation.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation.
Claims (6)
A fermentation step of adding yeast to the green tea extract to ferment;
An inactivating step of inactivating the yeast by heat-treating the yeast fermentation broth obtained in the fermentation step;
And filtering the supernatant obtained by centrifuging the yeast fermentation broth after the inactivation step to remove residues of the yeast fermentation broth.
A cosmetic composition using yeast fermentation characterized by being hypoallergenic to the skin.
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| CN109694886A (en) * | 2017-10-19 | 2019-04-30 | 安琪酵母股份有限公司 | A kind of green tea fermentation filtrate and its preparation method and application |
| KR20210104456A (en) * | 2020-02-17 | 2021-08-25 | 오가닉브릿지 주식회사 | Cosmetic materials composition for skin anti-oxidant and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof |
| KR102292975B1 (en) * | 2020-02-17 | 2021-08-26 | 오가닉브릿지 주식회사 | Cosmetic materials composition for skin anti-wrinkling and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof |
| KR102298533B1 (en) * | 2020-10-30 | 2021-09-07 | 주식회사 아미코스메틱 | Antianging cosmetic composition comprising green tea fermented extract fermented by inoculating lactococcus lactis strain |
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| CN109694886A (en) * | 2017-10-19 | 2019-04-30 | 安琪酵母股份有限公司 | A kind of green tea fermentation filtrate and its preparation method and application |
| CN109694886B (en) * | 2017-10-19 | 2022-07-01 | 安琪纽特股份有限公司 | Green tea fermentation filtrate and preparation method and application thereof |
| KR20210104456A (en) * | 2020-02-17 | 2021-08-25 | 오가닉브릿지 주식회사 | Cosmetic materials composition for skin anti-oxidant and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof |
| KR102292975B1 (en) * | 2020-02-17 | 2021-08-26 | 오가닉브릿지 주식회사 | Cosmetic materials composition for skin anti-wrinkling and skin-soothing, Cosmetic materials using the same and Manufacturing method thereof |
| KR102298533B1 (en) * | 2020-10-30 | 2021-09-07 | 주식회사 아미코스메틱 | Antianging cosmetic composition comprising green tea fermented extract fermented by inoculating lactococcus lactis strain |
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