KR101603956B1 - Cosmetic composition for skin with fermented extract of green tomato and Armillaria mellea - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an extract of a green tomato mushroom ( Armillaria mellea ) fermented extract, wherein the green tomato mulberry mushroom fermented extract of the present invention increases tomatidine, reduces skin irritation, improves skin wrinkles, , It is effective in enhancing skin barrier function.

Description

[0001] The present invention relates to a cosmetic composition comprising a green tomato mulberry mushroom fermented extract as an active ingredient,

The present invention relates to a cosmetic composition containing an extract of Armillaria mellea fermented with green tomato mushroom ( Armillaria mellea ) as an active ingredient. More specifically, the present invention relates to a cosmetic composition comprising a green tomato extract and a fermented extract having increased tomatidine content by fermenting with mulberry mushroom mycelium As an active ingredient.

Skin aging can be caused by active oxygen species activated by ultraviolet rays, stress, disease states, environmental factor scars, and aging. When such conditions are intensified, the antioxidant defense network existing in the living body is destroyed, and cells and tissues are damaged, and skin aging is promoted. Specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. The oxidation of proteins causes severe hyperinflammation by cleaving collagen, hyaluronic acid, elastin, proteoglycan and fibronetin, which are skin connective tissues, Will influence. If it gets worse, DNA mutation will lead to mutation, cancer induction, and immune function deterioration.

Therefore, it is necessary to protect the cell membrane by abolishing the active oxygen species that are mediated by the active oxygen species, the ultraviolet ray irradiation, and the inflammation reaction that occur during the metabolism of the body, and regenerate the already damaged cells by the active metabolism, Can be restored and healthy skin can be maintained.

On the other hand, the skin is an organ that functions as a protective film for protecting the living body from the external environment, and serves to prevent loss of biocomponents in the human body, that is, water and electrolytes, and to prevent harmful substances from entering the human body from the outside. The skin is divided into the skin layer, the dermal layer and the subcutaneous fat layer.

 The epidermal layer consists of keratinocytes and melanocytes. Since the keratinocytes in the outermost layer of the skin are in direct contact with the external environment, they require strong resistance to physical, chemical, or material permeation and prevent moisture from being lost to the outside At the same time as zooming, it is necessary to maintain the skin barrier and flexibility by holding proper moisture by itself. Generally, the moisture content of the skin is about 70% in the dermis, but when degeneration occurs in collagen fibers and elastic fibers in the dermis due to various factors including sunlight, skin moisture decreases and the elasticity of the skin is lowered. Is made. In addition, some subcutaneous fat is absorbed and wrinkles become worse.

It is known that the keratinocyte layer has a hydrophilic water retention ability called "Natural Moisturizing Factor (NMF)" and plays an important role in moisturizing the skin. Normal keratinocyte layer maintains skin smoothness and softness, and body protection function normally when moisture is maintained 10 ~ 30%. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the protective function of the body is lost and the aging phenomenon occurs. For example, in the case of dry skin, the keratinocyte aggregation is weakened, resulting in a scaling phenomenon in which keratinocytes are peeled off from the skin surface like scaly pieces. The reason why the skin is dried in this way is that the moisture content of keratinocyte layer is smaller than that of normal skin. In addition to this, even healthy skin may be in a harsh environment, such as wind, cold weather, sunshine, cleansing, shaving, etc., which causes water shortage, which can lead to poor skin condition.

Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer. To this end, cosmetics were added with ingredients similar to sebum, natural moisturizing factors, or moisturizers such as polyols. For example, glycerin or sorbitol having three or more hydroxyl groups as a water-soluble polyol exhibits excellent moisture resistance, but it is very sticky and feels uncomfortable when used. Propylene glycol 1,3-butylene glycol having two hydroxyl groups, . In addition, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, urea, etc., are highly electrolytic, which deteriorates emulsion stability of the cosmetic. Amino acids, collagen, and ellastin also have the ability to moisturize, but there was a limited ability to moisturize.

The ability of the horny layer to retain moisture can be controlled by natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. And the development of a substance with high moisturizing effect is one of the important research subjects in cosmetics.

Meanwhile, in recent years, many cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased.

The green tomato of the present invention is also referred to as an immature tomato. It has a tomato glycoalkaloid having a steroid skeleton. The tomato tomato glycoalkaloid has a glycoside-type alpha- (α-tomatine) and a small amount of dehydrotomatine (dehydrotomatine) is divided into. These glycosides are known to act as toxic to microorganisms or insects and protect plants from the outside. Recently, animal experiments have shown that they have various physiological activities such as cancer cell proliferation inhibitory effect, cholesterol lowering effect, and plant antibacterial activity .

On the other hand, Armillaria mellea is an exogenous mushroom which grows on stump and dead branches, such as under coniferous trees and broad-leaved trees, from spring to autumn. Mulberry mushroom is an edible mushroom that is known to be effective for geriatric diseases, stroke, dizziness, headache, nervous breakdown, insomnia, limb paralysis, polio, and epilepsy.

However, development of a skin cosmetic composition containing the green tomato and mulberry mushroom at the same time has not been developed, and accordingly, research is needed.

Korean Patent No. 10-0803924 discloses a cosmetic composition for preventing skin aging comprising an extract of tomato, grape and safflower seed supercritical fluid as an active ingredient. The composition of the present invention includes tomatoes (Lycopersicon esculentum), grape (Vitis vinifera) and safflower seeds Seeds of Carthamus tinctorius) as an active ingredient in a cosmetic composition for skin moisturizing and skin aging prevention. However, the green tomato mulberry mushroom fermentation extract prepared by fermenting green tomato extract with mycelium of Armillaria mellea has not been described.

The present invention aims to provide a cosmetic composition containing green tea mulberry mushroom fermented extract as an active ingredient and having effects of alleviating skin irritation, improving skin wrinkles, moisturizing skin, and enhancing skin barrier function.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a green tomato mulberry mushroom fermented extract as an active ingredient.

On the other hand, in the cosmetic composition of the present invention, the tomato mulberry mushroom fermentation extract is preferably fermented for 1 to 7 days with Mulberry Mushroom bacteria while maintaining the pH of the green tomato extract at 20 to 40 ° C at a pH of 5 to 7 . More preferably, the green tomato extract is added to the fermentation broth obtained by fermenting with Mulberry Mushroom Microorganism for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 ° C, water, anhydrous or lower alcohol having 1 to 4 carbon atoms , And acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. When the above range is satisfied, it is effective in relieving skin irritation, improving skin wrinkles, moisturizing skin, and strengthening skin barrier function.

On the other hand, in the cosmetic composition of the present invention, the above green tomato extract can be preferably extracted by adding ethanol to the green tomato crushed product. It is more preferable to add ethanol aqueous solution having an ethanol content of 50 to 90% (V / V) to extract.

Meanwhile, in the cosmetic composition of the present invention, the green tomato mulberry mushroom fermented extract preferably contains 0.0001 to 80.0% by weight based on the total weight of the cosmetic composition. When the above range is 0.0001 or less, it is not economical when the effect of alleviation of skin irritation, improvement of skin wrinkles, skin moisturization and skin barrier function is insignificant and is 80.0 or more.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition may be, for example, for alleviating skin irritation, improving skin wrinkles, moisturizing skin or strengthening skin barrier function.

On the other hand, in the cosmetic composition of the present invention, the suitable amount of the extraction solvent is 1 to 15 times, more preferably 7 times, the weight of the dried green tomato.

On the other hand, in the cosmetic composition of the present invention, the green tomato extract may be prepared in a powder state by an additional process such as vacuum distillation, freeze drying, spray drying and the like.

Meanwhile, the 'extract' referred to in the present invention means a supernatant obtained by removing the residue by using centrifugation after extraction.

Meanwhile, the cosmetic composition of the present invention may contain, as an active ingredient, the components commonly used in cosmetic compositions in addition to the green tomato mulberry mushroom fermentation extract described above, and may be used as an antioxidant, a stabilizer, a dissolving agent, a vitamin, a pigment, A conventional adjuvant, and a carrier.

Meanwhile, in the cosmetic composition of the present invention, the cosmetic composition may be a solution, suspension, emulsion, paste, gel, cosmetic composition, lotion, powder, soap, surfactant- , A wax foundation, and a spray.

When the formulation of the cosmetic composition of the present invention is a paste, a cosmetic composition or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, Zinc and the like can be used.

On the other hand, when the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Propane / butane, or dimethyl ether. ≪ RTI ID = 0.0 >

On the other hand, when the formulation of the cosmetic composition of the present invention is a solution or an emulsion, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used.

On the other hand, when the formulation of the cosmetic composition of the present invention is in the form of a suspension, the carrier component may include water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol , polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Suspending agents, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like may be used.

On the other hand, when the formulation of the cosmetic composition of the present invention is a cleansing agent containing an interfacial active agent, it is preferable to use, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The cosmetic composition containing the green tomato mulberry mushroom fermented extract of the present invention as an active ingredient is effective for relieving skin irritation, improving skin wrinkles, moisturizing skin, and enhancing skin barrier function. .

FIG. 1 is a graph showing the results of high performance liquid chromatography (HPLC) of the green tomato extract before (a) and after (b) fermentation of Mulberry Mushroom.

Hereinafter, the contents of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited to the following examples, but includes modifications of equivalent technical ideas.

[Preparation Example 1: Preparation of Green Tomato Extract]

The green tomatoes were washed with purified water, dried and then pulverized, and a fine green tomato crushed product was prepared using a sieve of 100 mesh. 70% (v / v) ethanol was added thereto so that the density of the green tomato pulp was 100 g / L, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper. After filtration, the filtrate was concentrated under reduced pressure at 50 DEG C or below and lyophilized to prepare a green tomato extract.

[Example 1: Preparation of green tomato mulberry mushroom fermentation extract]

To 5 g of the green tomato extract prepared in Preparation Example 1, 2% of glucose was added as a carbon source, 0.5% of peptone was further added, and sterilized. After sterilization, the mulberry mushroom culture solution was inoculated so as to have a total density of 25 g / L. Fermentation was carried out for 7 days using a 5 L fermenter at 37 ° C and maintained at pH 5-7. After fermentation, the culture broth was centrifuged to remove the culture medium first, and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the culture was firstly removed, the green tomato fermentation product was refluxed for 3 hours for 5 hours with addition of ethanol so as to be a final 70% (v / v) ethanol aqueous solution, followed by cooling. After cooling, it was filtered through a filter of whatman # 3. After filtration, the obtained extract was transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 DEG C or lower to prepare a green tomato mulberry mushroom fermented extract.

[Comparative Example 1: Preparation of fermentation broth of Mulberry Mushroom without green tomato extract]

In this comparative example, a fermentation broth of Mulberry Mushroom without green tomato extract was prepared.

2% of glucose was added as a carbon source, and 0.5% of peptone was further added to sterilize. The fermented broth was inoculated with a mulberry mushroom culture solution so that the total density reached 25 g / L. The fermentation was carried out for 7 days using a 5 L fermenter at 37 ° C and maintained at pH 5-7. After fermentation, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

After the culture was firstly removed, ethanol was added to make a final 70% (v / v) aqueous ethanol solution, and the mixture was refluxed three times for 5 hours, followed by cooling. After cooling, it was filtered through a filter of whatman # 3. When the filtration was completed, the filtrate was transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 ° C or lower to prepare a fermented broth of Mulberry Mushroom without green tomato extract.

[compare Yes  2 to 5: Preparation of Green Tomato Fermented Extract]

A green tomato fermented extract was prepared in the same manner as in Example 1, except that the green tomato extract of Preparation Example 1 was fermented using the bacterium of Table 1 instead of Mulberry Mushroom.

Type of bacteria Comparative Example 2 Yeast ( Saccharomyces cerevisiae ) Comparative Example 3 Lactobacillus casei ) Comparative Example 4 Asperillus oryzae ) Comparative Example 5 Bacillus subtilis (bacillus subtilis )

[ Experimental Example  One: Tomatidine ( tomatidine ) analysis]

In this experiment, the tomatidine contents of the green tomato extracts of Preparation Example 1 and the green tomato fermentation extracts of Comparative Examples 2 to 5 and the green tomato mulberry mushroom fermentation extract of Example 1 were measured by high performance liquid chromatography liquid chromatography (HPLC).

25 mM potassium phosphate (KH ₂ PO ₄ , pH 2.5) was used as the mobile phase. The analytical column used was OptiPack C18, the column temperature was 30 ° C and the ultraviolet detector absorbance wavelength was 254 nm.

The content of tomatidine in the green tomato mulberry mushroom fermentation extract of Example 1 and the green tomato mulberry mushroom fermentation extracts of Comparative Examples 2 to 5 were measured and shown in Table 2.

Before fermentation (a) (ppm) After fermentation (b) (ppm) Example 1 (Fermentation of Mulberry Mushroom Bacteria) 2,804.3 18,529.2 Comparative Example 2 (yeast fermentation) 2,804.3 2,804.53 Comparative Example 3 (fermentation of lactic acid bacteria) 2,804.3 2,802.31 Comparative Example 4 (fermentation of M. tuberculosis) 2,804.3 2,804.51 Comparative Example 5 (Bacillus subtilis fermentation) 2,804.3 2,805.32

As a result, the content of tomatidine before and after the fermentation of Mulberry mushroom in green tomato extract was 2,804.3 ppm before fermentation but increased to 18,529.2 ppm after fermentation

No significant increase in the amount of tomatidine was observed in other mycelial fermentations (Table 2).

The results of the analysis of the tomatidine content of the green tomato extract before (a) fermentation of Mulberry Mushroom and after fermentation of Mulberry Mushroom (b) are shown in FIG.

[ Experimental Example  2: Antioxidant assay of green tomato fermented extract DPPH Evaluation of antioxidant activity by

0.002, 0.005, 0.01% by weight of dry weight) of the green tomato extract of Preparation Example 1 and the green tomato fermentation extracts of Comparative Examples 2 to 5 were added to the free radical scavenging (Free radical scavenging acticity).

The antioxidant assay (DPPH assay) is a method of measuring the absorbance at 540 nm of the extent to which the inhibitor destroys the stable DPPH radical (2,2-Diphenyl-1-picrylhydrazyl radical). The used samples are shown in Table 3 below, and the free radical scavenging activity was measured using the following equation (1). The control group was Epigallocatechin gallate (EGCG).

material Control group Blank of C Exp. Group Blank of E A DPPH (MeOH) 180 μl (180) μl 180 μl (180) μl B Sample (solvent) (20) μl (20) μl 20 μl 20 μl

[Equation 1]

A: Abs of experimental group

B: Abs blank of experimental group

C: Abs of control group

D: Abs of blank of control group

Production Example 1 Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 EGCG 25uM - - - - - - - 53.44 0.001 wt% 13.22 45.21 3.94 23.21 14.55 11.33 21.91 - 0.002 wt% 21.62 64.82 6.90 30.90 21.92 21.72 24.28 - 0.005 wt% 25.84 84.13 8.21 34.28 34.11 32.13 36.45 - 0.01 wt% 34.25 98.55 9.90 51.95 42.81 44.24 44.52 -

(unit : %)

As shown in Table 4, the fermented extract of Mulberry Mushroom (Example 1) exhibited the highest free radical scavenging activity among the fermented extracts. In addition, excellent antioxidative effect was also confirmed in comparison with Comparative Example 1, which contained only fermented Mulberry Mushroom.

Therefore, it was confirmed that the green tomato mulberry mushroom fermented extract of the present invention is superior in antioxidative effect to the green tomato extract.

[Experimental Example 3: Evaluation of cell viability of green tomato mulberry mushroom fermented extract]

In this experiment, the toxicity of green tomato mulberry mushroom fermentation extract to cells was measured. Cytotoxicity was assessed by measuring the concentration of 3- (4,5-dimethylthiazol-2-yl) -2-5-methyl-thiazol- diphenyltetrazolium bromide, MTT) reagents to modify the cell viability of Mosmann.

Human dermal fibroblast (HDF) was dispensed in a 96-well plate at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C and 5% CO ₂ for 24 hours. After the culture medium was removed, the sample was cultured in a concentration-dependent medium for 24 hours. The medium was then removed and washed twice with phosphate-buffered saline (PBS). MTT was dissolved in PBS at 5 mg / mL, and 50 μL was added. The cells were incubated at 37 ° C and 5% CO ₂ for 2 hours. 100 μL / well of dimethyl sulfoxide (DMSO) was added, and the mixture was stirred for 10 minutes and absorbance was measured at 540 nm.

The measurement results are shown in Table 5 below. Table 5 shows that no cytotoxicity was observed at all concentrations of each sample. From this, it can be confirmed that the green tomato mulberry mushroom fermented extract of the present invention is harmless to the human body and has excellent safety.

Name of sample Treatment concentration (PPM) Cell survival rate (%) Production Example 1 50 103.1 100 105.3 Example 1 50 105.3 100 105.2 Comparative Example 1 50 98.9 100 101.4 Comparative Example 2 50 97.8 100 100.2 Comparative Example 3 50 98.9 100 103.8 Comparative Example 4 50 97.9 100 98.4 Comparative Example 5 50 98.2 100 101.4

[ Experimental Example  4: Assessment of skin irritation mitigation efficacy of green tomato mulberry mushroom fermentation extract (5-Lipoxygenase inhibition)]

In this experiment, the effect of 5-Lipoxygenase inhibitory activity of green tomato mulberry mushroom fermentation extract was evaluated.

Since lipoxygenase produces various substances acting as various chemical mediators involved in inflammation reaction and allergic reaction in vivo from arachidonic acid, substances which inhibit lipoxygenase are produced by the production of various chemical mediators And thus can be estimated to be effective for allergy. The degree of activity of lipoxygenase was measured by the degree of peroxide formation using substrate and enzyme.

The degree of activity of the lipoxygenase was determined by measuring the degree of peroxide formation using a substrate and an enzyme, and the inhibitory activity rate was measured using the following equation (2).

The samples used are shown in Table 6 below, and NDGA (Nordihydroguaiaretic acid) was used as the control group.

&Quot; (2) "

(%) = [1- (A-B) / (C-D)] 100

A: Abs of experimental group

B: Abs blank of experimental group

C: Abs of control group

D: Abs of blank of control group

material Control group Blank of C Exp. Group Blank of E A Buffer solution 2 ml 2 ml 2 ml 2 ml B Sample (solvent) (20 [mu] l) (20 [mu] l) 20 μl 20 μl C Enzyme 40 μl - 40 μl - D Substrate 70 μl 70 μl 70 μl 70 μl

Production Example 1 Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 NDGA 250 nM - - - - - - - 55.51 0.001 wt% 12.99 39.13 2.53 24.15 25.11 23.91 31.50 - 0.002 wt% 24.89 44.81 5.30 31.20 33.15 35.41 33.25 - 0.005 wt% 31.36 63.02 5.32 36.14 37.66 39.86 36.22 - 0.01 wt% 32.09 73.34 5.47 45.39 43.14 44.30 41.58 -

                                            (unit : %)

As shown in the results of Table 7, the green tomato mulberry mushroom fermentation extract (Example 1) was superior to the green tomato extract (Preparation Example 1) and the Mulberry Mushroom fermentation broth (Comparative Example 1) in inhibiting 5-lipoxygenase .

In general, the higher the inhibitory effect of 5-lipoxygenase, the more effective the skin irritation mitigation effect. Therefore, the above results show that the green tomato mulberry mushroom fermented extract has superior skin irritation mitigation effect as compared with the common green tomato extract.

[Experimental Example 5: Evaluation of skin wrinkle improving effect of green tomato mulberry mushroom fermentation extract]

In this experiment, the effect of increasing the collagen biosynthesis of green tomato mulberry mushroom fermentation extract was evaluated to evaluate skin wrinkle improving effect.

Human dermal fibroblast (HDF) was added to a 24-well plate at a concentration of 5 x 10 ⁴ cells / well and cultured at 37 ° C and 5% CO ₂ for 24 hours. Serum-free animal cell culture medium (DMEM medium) supplemented with the extracts prepared in Preparation Example 1, Example 1 and Comparative Examples 1 to 5, and a control serum-free animal cell culture medium (DMEM medium) Lt; / RTI > for 24 hours.

After the incubation, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured using a collagen kit (Takara, Japan), and the amount of synthesized collagen was measured . Collagen biosynthesis increase rate (%) was calculated according to the following equation (3), and L-ascorbic acid (L-AA) was used as a control.

&Quot; (3) "

Production Example 1 Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 L-AA 0.3mM - - - - - - - 133.16 0.001 wt% 101.12 125.76 102.12 107.01 115.14 106.59 114.38 - 0.002 wt% 106.17 133.82 104.65 113.05 116.53 117.74 127.63 - 0.005 wt% 114.56 148.44 105.46 120.06 122.25 124.32 129.16 - 0.01 wt% 116.62 163.52 107.45 128.74 124.16 128.25 135.23 -

(unit : %)

As shown in the results of Table 8 above, the green tomato mulberry mushroom fermented extract of the present invention (Example 1) had an excellent amount of collagen synthesis as compared with Preparation Example 1 and Comparative Examples 1 to 5.

Here, an excellent amount of collagen synthesis means that it is effective for improving skin wrinkles.

[ Manufacturing example  2: green tomato mulberry mushroom fermented extract as an active ingredient Cosmetic  Produce]

The cosmetic composition containing 20.0% by weight of the green tomato mulberry mushroom fermented extract of Example 1 was prepared with the composition shown in the following Table 8 and referred to as Prescription Example 1.

 On the other hand, a cosmetic composition comprising the green tomato extract of Preparation Example 1 was prepared in Comparative Preparation Example 1, and a cosmetic composition containing only a fermentation broth of Mulberry Mushroom of Comparative Example 1 was prepared and referred to as Comparative Prescription Example 2.

ingredient Prescription Example 1
(weight%) Comparative Prescription Example 1
(weight%) Comparative Prescription Example 2
(weight%) Green Tomato Extract
(Production Example 1) - 20.0 - Green tomato mulberry mushroom fermented extract (Example 1) 20.0 - - Mulberry mushroom fermentation broth
(Comparative Example 1) - - 20.0 1,3-BG 10.0 10.0 10.0 glycerin 5.1 5.1 5.1 Propylene glycol 4.2 4.2 4.2 Tocopheryl acetate 3.0 3.0 3.0 Liquid paraffin 4.6 4.6 4.6 Triethanolamine 1.0 1.0 1.0 Squalane 3.1 3.1 3.1 Macadamia nut oil 2.5 2.5 2.5 Polyahyuvait 60 1.6 1.6 1.6 Azithromycin 1.6 1.6 1.6 Propylparaben 0.6 0.6 0.6 Carboxyl vinyl polymer 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount Purified water Balance Balance Balance system 100 100 100

[ Experimental Example  6: Skin of green tomato mulberry mushroom fermentation extract Moisture power  Improvement effect]

This experiment was carried out to examine the skin moisturizing effect of the 'cosmetic composition containing the green tomato mulberry mushroom fermented extract as an active ingredient' prepared in Preparation Example 2 above. The cosmetic composition of Preparation Example 1 and Comparative Preparation Example 1 prepared in Preparation Example 2 was divided into two groups each consisting of 20 persons per one group of 20 to 40 years without skin disease, Face and forehead.

The skin conductivity was measured using a moisture analyzer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application and the skin conductivity increase rate after 1 week, 2 weeks, %) Were measured and the rate of increase was evaluated. The results are shown in Table 10 below.

sample After 1 week after 2 weeks After 4 weeks Prescription Example 1 51 57 62 Comparative Prescription Example 1 26 33 38 Comparative Prescription Example 2 17 20 24

(unit : %)

As shown in the results of Table 10, in the case of Prescription Example 1, which is a cosmetic composition containing the fermented extract of green tomato mulberry mushroom as an active ingredient of the present invention, the rate of increase in skin conductivity is higher than that of Comparative Prescription Examples 1 and 2 Respectively.

Generally, the skin conductivity is proportional to the skin moisture content, so that the above results indicate that the cosmetic containing the green tomato mulberry mushroom fermented extract as an active ingredient maintains higher skin moisture content than the other cosmetic ingredients.

[ Experimental Example  7: Enhancement of skin barrier function of green tomato mulberry mushroom fermentation extract]

This experiment was conducted to examine the skin barrier function enhancing effect of the 'cosmetic composition containing the green tomato mulberry mushroom fermented extract as an active ingredient' prepared in Preparation Example 2 above.

For 40 women in their 20s and 40s, apply each sample to the right and left sides, and apply 1, 2, 4, or 6 hours of transdermal water before applying the area 1 cm below the right side of the eye 3 cm The loss was measured three times using a Tewameter (TM300, Courage and Khazaka Electronic Co., Germany) and the average value was used to evaluate the amount of percutaneous water loss.

division 1 hours 2 hours 4 hours 6 hours Prescription Example 1 15.9 15.4 13.1 9.9 Comparative Prescription Example 1 17.2 16.3 13.4 11.7 Comparative Prescription Example 2 18.2 16.8 15.0 14.4

(Unit: g / h / m ² )

Experimental results showed that the amount of transdermal water loss was reduced in the facial skin of the applicant who applied the cosmetic composition containing the green tomato mulberry mushroom fermented extract as an active ingredient. Therefore, it was found that it is effective in strengthening the skin barrier function (Table 11).

Claims (10)

Green tomato mulberry mushroom ( Armillaria mellea ) fermented extract as an active ingredient.
The method according to claim 1,
The green tomato mulberry mushroom fermentation extract,
Wherein the green tomato extract is fermented with mulberry mushroom bacteria for 1 to 7 days while maintaining a pH of 5 to 7 under a temperature condition of 20 to 40 占 폚.
3. The method of claim 2,
The green tomato extract,
Wherein the extract is an extract obtained by adding ethanol to the ground green tomato powder.
The method of claim 3,
The above-
Wherein the aqueous solution is an aqueous ethanol solution having an ethanol content of 50 to 90% (V / V).
3. The method of claim 2,
The green tomato mulberry mushroom fermentation extract,
The green tomato extract was fermented for 1 to 7 days with Mulberry Mushroom bacterium under the temperature condition of 20 ~ 40 ℃,
Wherein the extract is an extract obtained by adding any one of extraction solvents selected from the group consisting of water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol. Composition.
The method according to claim 1,
The green tomato mulberry mushroom fermentation extract,
0.0001 to 80.0% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for alleviating skin irritation.
The method according to claim 1,
In the cosmetic composition,
Wherein the cosmetic composition is for improving skin wrinkles.
The method according to claim 1,
In the cosmetic composition,
Wherein the composition is for moisturizing the skin.
The method according to claim 1,
In the cosmetic composition,
Wherein the composition is for enhancing skin barrier function.

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