KR100510104B1 - Fermentative Extract of Rooibos and Cosmetic Compositions Comprising the Same for Preventing Skin Aging - Google Patents

Abstract

The present invention relates to a rooibos fermentation extract excellent in active oxygen species removal efficacy and a cosmetic composition for preventing skin aging comprising the extract as an active ingredient, the cosmetic composition of the present invention is a reactive oxygen species generated by ultraviolet irradiation, in particular super By effectively removing the oxide, it is possible to inhibit the peroxidation of the cell membrane lipids by the reactive oxygen species, resulting in preventing aging of the skin.

Description

Fermentative Extract of Rooibos and Cosmetic Compositions Comprising the Same for Preventing Skin Aging}

The present invention relates to a rooibos fermentation extract and a cosmetic composition comprising the same, more specifically, rooibos fermentation extract (Rooibos) fermentation extract excellent in active oxygen species removal efficacy, a method for preparing the fermentation extract and the fermentation extract as an active ingredient It relates to a cosmetic composition for preventing skin aging.

The primary function of the skin is to protect the human body from external stimuli such as temperature, humidity and ultraviolet rays. However, when the skin is exposed to external stimuli such as strong ultraviolet rays, stress, and malnutrition, the skin may age, such as wrinkle formation, loss of elasticity, and keratinization.

Generally, there are many theories explaining skin aging, but among them, the free radical theory that skin ages due to free radicals generated by various causes both inside and outside the body is accepted as the most valid theory. Free radicals are chemically highly reactive chemicals, which are produced by various causes in the human body: external factors such as ultraviolet rays, radiation, exhaust gases, cigarette smoke, stress, and metabolic processes in the body's mitochondria and white blood cells. In addition, multi-step chain reactions lead to peroxidation of lipids, which are components of cell membranes.

Most of the free radicals associated with skin aging are Reactive Oxygen Species (ROS), which include super oxide radicals, hydrogen peroxide (H2O2), hydroxy radicals, single Singlet oxygen; The peroxidation of lipids caused by them generates various active species such as radicals, peroxides, aldehydes and epoxides, and damages DNA, RNA, proteins, cell membranes and cellular structures.

These reactive oxygen species are thought to be the main cause of cancer, tissue damage, and aging (Black HS, Photochemphotobiol. 46 (2): 213 (1987)). For example, the peroxidation of the membrane lipids that make up the cell can change the structure and fluidity of the cell membrane, increase the permeability of the cytoplasm, leak the lysosomal enzyme, inactivate the membrane enzyme and the transport protein, create covalent crosslinks of lipids and proteins, and It causes chain breakage, DNA damage and mutations. Among these reactions, peroxidation of dermal cell membranes and modification of dermal component proteins are known to be most closely associated with skin aging.

Aerobic organisms have protective mechanisms to prevent the formation of these oxidants and lipid peroxidation. These protective mechanisms include enzymes such as super oxide dismutase (SOD) and catalase, tocopherol (vitamin E), β-carotene and ascorbic acid (ascorbic acid, vitamin). C) and fat soluble and water soluble antioxidants or radical scavengers such as glutathione.

As described above, since free radical type oxygen or reactive oxygen species are highly related to skin aging, conventionally, superoxide dismutase, vitamin E derivatives, vitamin C and its derivatives, flavonoids, and the like are applied to cosmetics. Although a lot of efforts have been made to achieve the anti-aging effect by blending, in the case of blending the above ingredients in cosmetics, it is difficult to expect a substantial effect because of poor safety and stability. Therefore, there is a need to solve the above-mentioned problems of the prior art and to develop a product having excellent active oxygen removal ability.

Rooibos, on the other hand, is a conifer that lives only in the Cedar Berg Plateau in Cape of Good Hope, South Africa. Asparathus Linearis , the tree is a shrub belonging to 1-2 m in height, 8-10 m in root, 2-6 cm in needle-shaped leaves, and a fruiting passage in the pod, It receives the sun's heat during the day, gets cold winds at night, and grows on dry, dry land.

In order to grow in such a harsh environment, it is a plant that grows strongly while taking roots deeply and absorbing various minerals. The benefits of rooibos are to improve various skin diseases, treat allergic symptoms (treatment of atopic dermatitis), normalize blood pressure, improve diabetes, relieve constipation and intestinal action, suppress aging, mental stability, diuretic effect, improve liver function, Antibacterial and bactericidal action is known.

Korean Patent Publication No. 2001-44746 discloses a rooibos moisturizer that is effective in skin diseases such as acne, atopic dermatitis, blemishes, and freckles, prepared by packaging rooibos and silicate in a nonwoven fabric in a certain amount. -429 discloses a method for extracting an active ingredient effective in treating acne, atopic dermatitis and various skin diseases from leaves and young stems of rooibos, and a cosmetic using the extract as an active ingredient.

Throughout this specification, many papers and patent documents are referenced and their citations are indicated. The disclosures of cited papers and patent documents are incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors have diligently researched to develop a cosmetic composition having excellent active oxygen removing ability, and when the cosmetic composition contains fermented extract of rooibos as an active ingredient, the active oxygen removing ability and lipid peroxidation inhibitory ability are improved, thereby preventing skin aging. The present invention has been completed by confirming the effectiveness thereof.

An object of the present invention to provide a cosmetic composition for preventing skin aging comprising a rooibos fermented extract.

The present invention provides a cosmetic composition for preventing skin aging comprising a Rooibos fermentation extract having a superoxide removal ability as an active ingredient.

As used herein, the term "rooibos" refers to a plant having the scientific name Asparathus Linearis , and preferably means the drying of various organs of rooibos (such as leaves, flowers, stems, and roots). More preferably, it means that the leaves of rooibos are dried.

According to a preferred embodiment of the present invention, the fermentation extract included as an active ingredient in the cosmetic composition of the present invention is obtained by fermenting a mixture of rooibos, persimmon leaves and jasmine. Therefore, the term "rooibos fermented extract" in the present specification is a result obtained by the extraction process by fermentation by microorganisms and extraction with extraction solvent, the mixture containing rooibos, more preferably rooibos as a main raw material, and additionally persimmon leaves and jasmine Means. According to a more preferred embodiment of the present invention, the fermentation extract of the present invention is obtained by fermenting a mixture of 40-70% by weight of rooibos, 10-35% by weight of persimmon leaves and 5-25% by weight of jasmine.

In the above-mentioned rooibos fermented extract, the component derived from rooibos has the antioxidant action of the cosmetic composition of the present invention, more specifically, the antioxidant action through the removal of free radical species (free radical type oxygen), and more particularly the active oxygen. It is a major component of antioxidant species through the removal of superoxide. This antioxidant action is achieved by, for example, superoxide dismutases (hereinafter referred to as “SOD”) and flavonoids derived from rooibos.

Persimmon leaves and jasmine have flavonoids and vitamin C. These are antioxidants, so they're included. It is also known that the fragrance component of jasmine has an aroma therapeutic function to calm nerves.

According to a preferred embodiment of the present invention, the fermented extract of the present invention comprises the steps of: (a) preparing a fermentation raw material selected from the group consisting of rooibos, a mixture of rooibos and persimmon leaves and a mixture of rooibos, persimmon leaf and jasmine; (b) fermenting the fermentation raw material with a microorganism having a high yield of alpha amylase and protease to obtain a fermentation product; And (c) the fermented product is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g. methanol, ethanol, propanol and butanol, etc.), a mixed solvent of the lower alcohol with water, acetone, ethyl acetate, chloroform, Obtained by a method comprising extracting with an extraction solvent selected from the group consisting of 1,3-butylene glycol, hexane and diethyl ether.

In the above fermentation and extraction process, it is preferable to adjust the moisture content of the fermentation raw material to 40-60% by weight before fermenting the fermentation raw material to microorganisms, for the purpose of ensuring that the microorganisms inoculated into the fermentation raw material are well cultured. . It is also desirable to sterilize fermentation raw materials with adequately controlled moisture content before inoculating microorganisms. The sterilization method may be any sterilization method conventionally practiced in the art, and may be implemented, for example, through a high pressure sterilization method, a high temperature sterilization method, and a low temperature sterilization method. As a specific example, sterilization may be performed at a high temperature for 10 minutes to 30 minutes at 100 ° C. or higher (eg, 121 ° C.).

Microorganisms which ferment fermentation raw materials should be microorganisms with high yield of alpha amylase and protease. The reason is that alpha amylase is necessary for the fermentation of fermentation raw materials because the function of degrading the fiber present in a lot of plants, and the protease to increase the fermentation efficiency by proteolysis.

According to a preferred embodiment of the present invention, the microorganism used in the present invention is Aspergillus oryzae , Aspergillus niger or Aspergillus usamii , and most preferably Aspergillus usamii . It is Fergus's O'Rage. The microorganisms are known as edible microorganisms. Fermentation by the Aspergillus orisase is preferably performed at about 28-33 ° C. for about 24-80 hours.

The fermentation product obtained by the fermentation process is preferably extracted by an extraction solvent after a drying process (for example, drying for 15-50 hours at 40-70 ° C.). Extraction with an extraction solvent may be any extraction solvent conventionally used in the art, most preferably extraction with water. As can be seen in the following examples, the extraction using water is the best extraction yield. On the other hand, the amount of the extraction solvent may vary depending on the type of extraction solvent used, typically 1-20 times the dry weight of the fermentation raw material, preferably 1-10 times.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the rooibos fermented extract of the present invention. In addition, the rooibos fermented extract of the present invention may be prepared in powder form by an additional process such as vacuum distillation and freeze drying or spray drying.

The rooibos fermented extract prepared by the above method has an excellent ability to remove reactive oxygen species (free radical type oxygen), in particular, superoxide, which inhibits intracellular lipid peroxidation by the reactive oxygen species, which in turn inhibits skin aging. Do it.

In a preferred embodiment of the present invention, the content of rooibos extract is from 0.001 to 10.0% by weight, more preferably from 0.05 to 5.0% by weight based on the total cosmetic composition. If the content of the rooibos extract is less than 0.001% by weight, no obvious anti-aging effect, in particular, the removal of active oxygen species cannot be expected, and if the content of the rooibos extract is more than 10.0% by weight, the active oxygen species with the increased content There is no increase in the removal effect.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the rooibos fermentation extract as an active ingredient, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings, And carriers.

The composition for alleviating the skin irritation of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants -Can be formulated with, but not limited to, cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention has the use of skin anti-aging. As used herein, the term "anti-aging of skin" has the meaning encompassing comprehensively the prevention and treatment of skin aging. The cosmetic composition of the present invention inhibits lipid peroxidation through the removal of reactive oxygen species (particularly superoxides), which in turn prevents skin aging, and this fact is specified in the examples below. On the other hand, skin aging prevention, which is the use of the present invention, includes the inhibition of the condition of the skin derived from reactive oxygen species, such as skin aging and the formation of wrinkles (especially wrinkles caused by ultraviolet rays).

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1: Obtaining Rooibos Fermentation Powder

60% by weight of rooibos, 25% by weight of persimmon leaves and 15% by weight of jasmine were mixed and pulverized, and then the water content was adjusted to 50% by adding water in order to allow the fermentation microorganism to be well cultured to obtain a fermentation raw material.

Subsequently, the obtained fermentation raw material was sterilized at 121 ° C. for 20 minutes, inoculated with 3% by weight of Aspergillus orisase, and then fermented under aerobic conditions at 30 ° C. for 48 hours. Then, it was left to dry at 50 ° C. for 30 hours, and then ground to obtain rooibos fermented powder.

Preparation Example 2 Obtaining Rooibos Fermentation Extract Using Various Extraction Solvents

Preparation Example 2-a

1 kg of the fermented powder obtained in Preparation Example 1 was added to 5 L of water, and extracted at room temperature for 5 days. Then, the obtained extract was filtered through a 300 mesh filter cloth, concentrated under reduced pressure using a distillation apparatus equipped with a cooling condenser to remove the solvent, and dried to obtain a dry weight 123.8 g (yield: 12.3%) of rooibos fermented extract. It was.

Preparation Example 2-b

1 kg of the fermented powder obtained in Preparation Example 1 was added to 5 L of 10% (v / v) ethanol and extracted at room temperature for 3 days. Thereafter, the obtained extract was filtered through a 300 mesh filter cloth, concentrated under reduced pressure using a distillation apparatus equipped with a cooling condenser to remove the solvent, and dried to obtain a dry weight 120.7 g (yield: 12.0%) of rooibos fermented extract. It was.

Preparation Example 2-c

A dry weight of 121.7 g (yield: 12.1%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except using 20% (v / v) ethanol.

Preparation Example 2-d

A dry weight of 112.7 g (yield: 11.2%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except using 30% (v / v) ethanol.

Preparation Example 2-e

A dry weight 99.7 g (yield: 9.9%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except for using 40% (v / v) ethanol.

Preparation Example 2-f

A dry weight of 84.7 g (yield: 8.4%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except for using 50% (v / v) ethanol.

Preparation Example 2-g

Using the same method as Preparation Example 2-b, except using 60% (v / v) ethanol, a dry weight 67.7 g (yield: 6.7%) of rooibos fermented extract was obtained.

Preparation Example 2-h

A dry weight of 54.7 g (yield: 5.4%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except for using 70% (v / v) ethanol.

Preparation Example 2-i

Using the same method as Preparation Example 2-b, except using 80% (v / v) ethanol, a dry weight 43.7 g (yield: 4.3%) of rooibos fermented extract was obtained.

Preparation Example 2-j

Using the same method as Preparation Example 2-b, except using 90% (v / v) ethanol, a dry weight 25.7 g (yield: 2.5%) of rooibos fermented extract was obtained.

Preparation Example 2-k

Using the same method as Preparation Example 2-b, except using 100% (v / v) ethanol, a dry weight 17.7 g (yield: 1.7%) of rooibos fermented extract was obtained.

Preparation Example 2-l

A dry weight of 42.7 g (yield: 4.2%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except for using 80% (v / v) methanol.

Preparation Example 2-m

A dry weight of 16.7 g (yield: 1.6%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except for using 100% (v / v) methanol.

Preparation Example 2-n

A dry weight of 8.7 g (yield: 0.8%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except for using acetone.

Preparation Example 2-o

Using the same method as Preparation Example 2-b, except using ethyl acetate, a dry weight 11.7 g (yield: 1.1%) of rooibos fermented extract was obtained.

Preparation Example 2-p

A dry weight of 57.7 g (yield: 5.7%) of rooibos fermented extract was obtained using the same method as Preparation Example 2-b except for using 50% (v / v) 1,3 butylene glycol.

Preparation Example 2-q

Using the same method as Preparation Example 2-b except using hexane, a dry weight 8.7 g (yield: 0.8%) of rooibos fermented extract was obtained.

Preparation Example 2-r

Using the same method as Preparation Example 2-b except for using diethyl ether, a rooibos fermented extract having a dry weight of 3.7 g (yield: 0.3%) was obtained.

Preparation Example 2-s

Using the same method as Preparation Example 2-b except for using normal propanol, a rooibos fermented extract having a dry weight of 2.7 g (yield: 0.2%) was obtained.

Preparation Example 2-t

Using the same method as Preparation Example 2-b, except using isopropanol, a rooibos fermented extract of dry weight 2.7 g (yield: 0.2%) was obtained.

Preparation Example 2-u

A rooibos fermented extract of 2.7 g (yield: 0.2%) of dry weight was obtained using the same method as Preparation Example 2-b except for using normal butanol.

Experimental Example 1: Determination of free radical scavenging activity of rooibos fermented extract

When xanthine is reacted with Xanthin oxidase, superoxide is produced. At this time, the generated superoxide exhibits absorbance at 295 nm, and the amount of superoxide can be quantified as an increase in absorbance over time. If a substance capable of removing the superoxide is added to the reaction system, the superoxide is removed by the additive, indicating that the degree of increase in absorbance with time decreases. Therefore, the inhibition rate (%) can be obtained by calculating the degree of absorbance increase of the reaction system containing the additive in relation to the degree of absorbance increase of the reaction system containing no additives, and the superoxide removal ability can be calculated.

0.5 ml of xanthine and 0.3 ml (PH 7.5) of 50 mM phosphate buffer solution were mixed, and 0.1 ml of each of the preparation extracts were added. 0.1 ml of xanthine oxidase (Sigma, U.S.A.) was added and reacted at 25 ° C for 10 minutes, and then the absorbance was measured at 295 nm for 3 minutes. Inhibition of xanthine oxidase was calculated by using 100% of absorbance as the control without adding the extract. In addition, the free radical removal rate (%) was calculated by calculating the difference between the extract-free absorbance (O.D.295%) and the extract-added absorbance (O.D.295%).

extract Absorbance change rate (O.D. 295% of no addition) % Removal    No extract         100     0 Preparation Example 2-a 69 ± 2.1 31 Preparation Example 2-b 71 ± 0.5 29 Preparation Example 2-c 73 ± 0.8 27 Preparation Example 2-d 73 ± 1.2 27 Preparation Example 2-e 74 ± 1.5 26 Preparation Example 2-i 75 ± 2.3 25 Preparation Example 2-j 75 ± 3.4 25 Preparation Example 2-k 78 ± 2.9 22 Preparation Example 2-m 80 ± 3.8 20 Rooibos Water Extract * 91 ± 1.9 8 Rooibos 50% (v / v) ethanol extract ** 84 ± 3.0 16 Rooibos 100% (v / v) ethanol extract *** 83 ± 1.3 17 *, ** and ***: used as a control, without undergoing the fermentation step, only the extraction step according to the method described in Preparation Example 2.

As can be seen in Table 1, it can be seen that the free radical scavenging ability of the rooibos fermentation extract prepared through the fermentation process is greater than the free radical scavenging ability of the extract extracted directly from the rooibos without passing through the fermentation step, the rooibos fermentation of the present invention If the extract is included in the cosmetics is expected to have excellent antioxidant effect.

Experimental Example 2 Measurement of Inhibition of Intracellular Lipid Peroxidation by Free Radicals of Rooibos Fermented Extract

In the cell, lipids are peroxidated due to excessive free radicals. If a substance is added to a cell, after overproducing a free radical and quantifying the oxidized lipid in the cell, the amount of the oxidized lipid may vary depending on the added substance. In addition, the free radical production inhibition or lipid oxidation inhibition of the added substance can be measured indirectly.

Experimental Example 2-a: Culture of Fibroblasts and Ultraviolet Irradiation

Human normal fibroblasts (NHFB, Korea Cell Line Bank) were inoculated into nine 100 mm cell culture Petri dishes at 1 x 105 cells per dish and DMEM (Sigma) containing 10% (v / v) bovine serum Incubated at 37 ° C. and 5% O 2 for 3 days. At this time, 10 μg / ml of the rooibos fermented extract prepared in Preparation Examples 2-a, 2-b, 2-c, 2-d or 2-i to the medium was used as an experimental group, and the extract was added. Two dishes which were not prepared, a dish in which 10 µg / ml of rooibos extract was added, and a dish in which 10 µg / ml of persimmon leaf extract were added, were used as a comparison group. At this time, the rooibos extract or persimmon leaf extract is added 1 kg of rooibos powder or persimmon leaf powder to 5 l of 70% (v / v) ethanol, extracted for 3 days at room temperature, filtered with 300 mesh filter cloth, equipped with a cooling condenser Concentration under reduced pressure using a distillation apparatus to remove the solvent, dried to obtain each.

Subsequently, the medium was removed, and each dish was washed twice with a phosphate buffered saline (PBS) solution, and then 1 ml of PBS was added to each dish and irradiated with ultraviolet A at a light amount of 15 / cm 2. Then, after removing the PBS of each dish, each of the original culture of each dish was dispensed, and then further incubated for 24 hours. At this time, one of the two dishes in which no extract was added in the comparison group was not irradiated with ultraviolet A, and this was used as a control.

Experimental Example 2-b: Determination of Lipid Peroxides

Quantification of lipid peroxides produced in cells by treatment with ultraviolet light was performed using the reactivity of thiobarbithric acid (TBA) (Kari. P. et al, J. Invest. Dermatol. 96: 255-259 (1991). First, the cultured cells were trypsinized and separated from each petri dish, and the cells were then suspended in 1 ml of homogenization buffer solution (PBS, phosphate buffered saline) and homogenizer (homogenizer, DIAX × 900, Heidolph homogeniger). , Germany).

Then, 0.5 ml of 30% Trichloroacetic acid (TCA) solution and 50 µl of 2% butylated hydroxy toluene (BHT) ethanol solution were added to 0.5 ml of each cell grinding solution. Then, 0.5 ml of an aqueous solution of TBA (375 mg / ml) was added, heated in boiling water with a bath for 15 minutes, then cooled, and absorbance was measured at 535 nm. Based on the absorbance of the control group not treated with UV light, the absorbance increase rate of each experimental group and the comparative group was calculated, and the inhibition of peroxidation was calculated therefrom. The results are shown in Table 2 below.

extract Absorbance Increase (O.D.535% of Control) Peroxidation inhibition rate (%) Control 100 - No extract 155 ± 4.0 0 Rooibos extract 114 ± 3.5 26 Persimmon Leaf Extract 126 ± 3.8 19 Preparation Example 2-a 98 ± 2.3 36 Preparation Example 2-b 105 ± 2.5 32 Preparation Example 2-c 110 ± 0.5 29 Preparation Example 2-d 111 ± 4.1 28 Preparation Example 2-i 122 ± 3.4 21

As can be seen in Table 2, it can be seen that the rooibos fermented extract effectively inhibits the peroxidation of cell membrane lipids by free radicals generated by UV irradiation, and the rooibos fermented extract can be used for the purpose of preventing skin aging. Can be.

Formulation Example 1

Formulation example of the flexible lotion in the cosmetic containing the rooibos fermented extract of Preparation Example 2-a of the present invention is as follows.

ingredient Content (% by weight) Rooibos Ferment Extract 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2

Formulation example of the milk lotion in the cosmetic composition containing the rooibos fermented extract of Preparation Example 2-a is as follows.

ingredient Content (% by weight) Rooibos Ferment Extract 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 3

Formulation example of the nutritional cream in the cosmetic composition containing the rooibos fermented extract of Preparation Example 2-a is as follows.

ingredient Content (% by weight) Rooibos Ferment Extract 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 4

Formulation example of the massage cream in the cosmetic composition containing the rooibos fermented extract of Preparation Example 2-a is as follows.

ingredient Content (% by weight) Rooibos Ferment Extract 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 5

Formulation example of the pack in the cosmetic containing the rooibos fermented extract of Preparation Example 2-a of the present invention is as follows.

ingredient Content (% by weight) Rooibos Ferment Extract 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

As described in detail above, the present invention provides a cosmetic composition for preventing skin aging comprising a rooibos fermented extract as an active ingredient. The cosmetic composition of the present invention is excellent in removing free radical oxygen, especially superoxide, and is useful for preventing skin aging because it effectively inhibits lipid peroxidation.

Claims (9)

Rooibos with superoxide removal ability extracted by fermenting rooibos with Aspergillus oryzae , Aspergillus niger or Aspergillus usamii , microorganisms with high yield of alpha amylase and protease Cosmetic composition for preventing skin aging comprising a fermented extract as an active ingredient. The cosmetic composition for preventing skin aging according to claim 1, wherein the fermented extract is obtained by fermenting a mixture of rooibos, persimmon leaves, and jasmine. According to claim 2, wherein the fermentation extract is a skin anti-aging cosmetic composition, characterized in that obtained by fermenting a mixture of rooibos 40-70% by weight, persimmon leaves 10-35% by weight and jasmine 5-25% by weight. The cosmetic composition for preventing skin aging according to any one of claims 1 to 3, wherein the fermented extract is obtained by a method comprising the following steps: (a) preparing a fermentation raw material selected from the group consisting of rooibos, a mixture of rooibos and persimmon leaves and a mixture of rooibos, persimmon leaf and jasmine; (b) fermenting the fermentation raw material with a microorganism having a high yield of alpha amylase and protease to obtain a fermentation product; And (c) the fermented product is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, a mixed solvent of the lower alcohol with water, acetone, ethyl acetate, chloroform, 1,3-butylene glycol, hexane and diethyl ether Extracting using an extraction solvent selected from the group consisting of. The cosmetic composition for preventing skin aging according to claim 4, wherein the microorganism is Aspergillus oryzae , Aspergillus niger or Aspergillus usamii . The cosmetic composition for preventing skin aging according to claim 5, wherein the microorganism fermentation is performed at 28-33 ° C for 24-80 hours. The cosmetic composition for preventing skin aging according to claim 4, wherein the amount of the extraction solvent is 1-20 times the dry weight of the fermentation raw material. The cosmetic composition for preventing skin aging according to any one of claims 1 to 3, wherein the amount of the fermented extract is 0.001-10.0% by weight based on the total cosmetic composition. The method of claim 8, wherein the anti-aging cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and A skin anti-aging cosmetic composition, characterized in that it has a formulation selected from the group consisting of a spray.