JPH11116429A - Cosmetic stock and cosmetic - Google Patents
Cosmetic stock and cosmeticInfo
- Publication number
- JPH11116429A JPH11116429A JP9278451A JP27845197A JPH11116429A JP H11116429 A JPH11116429 A JP H11116429A JP 9278451 A JP9278451 A JP 9278451A JP 27845197 A JP27845197 A JP 27845197A JP H11116429 A JPH11116429 A JP H11116429A
- Authority
- JP
- Japan
- Prior art keywords
- water
- cosmetic
- extract
- tea leaves
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は化粧品原料および化
粧品に関し、特に皮膚に対して安全で美白作用のある化
粧品原料およびそれを用いた化粧品に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a raw material for cosmetics and cosmetics, and more particularly to a raw material for cosmetics having a safe and whitening effect on the skin and a cosmetic using the same.
【０００２】[0002]
【従来の技術】美白化粧品の原料物質として種々の物質
が知られているが、合成品は長期間人間の肌に適応した
場合の安全性の保証がなく、使用が制限されつつある。
一方、天然物では美白作用の弱いものが多い。しかし人
の肌に対する安全性の面から天然物で、多年、人が飲食
したりして、安全性の面で保証されており、更に皮膚に
対する美白作用や老化防止など他の効果を合わせてもつ
物質が望まれている。一方、アフリカ南部の酸性乾燥土
壌に成育するマメ科の植物で、学名アスパラサス・リネ
アリス（Aspalathus linealis ）またはアスパラサス・
セダルベルゲンシス（Aspalathus cedarbergensis ）の
乾燥葉はルイボス茶として長年アフリカ南部において飲
用されている。このルイボス茶に他の茶剤成分を配合し
た健康茶も知られている（特開平 6-70681号公報）。ま
た、アスパラサス・リネアリスの抽出液を洗浄剤成分と
する洗浄剤組成物が開示されている（特開平 6-136386
号公報）。2. Description of the Related Art Various materials are known as raw materials for whitening cosmetics. However, the use of synthetic products is not guaranteed for long-term adaptation to human skin, and their use is being restricted.
On the other hand, many natural products have a weak whitening effect. However, it is a natural product from the aspect of safety for human skin, and is guaranteed for safety by eating and drinking for many years, and has other effects such as skin whitening effect and anti-aging. A substance is desired. On the other hand, it is a leguminous plant that grows on acid dry soil in southern Africa, and has the scientific name Aspalathus linealis or Aspalathus linealis.
Dried leaves of Aspalathus cedarbergensis have long been consumed in southern Africa as rooibos tea. A health tea in which other tea ingredients are added to this rooibos tea is also known (Japanese Patent Application Laid-Open No. 6-70681). In addition, a detergent composition containing an extract of Asparasus linealis as a detergent component has been disclosed (JP-A-6-136386).
No.).
【０００３】[0003]
【発明が解決しようとする課題】しかしながら、ルイボ
ス茶葉から抽出された抽出物の化粧品原料としての抗炎
症性や、抗酸化性、活性酸素抑制効果などについては知
られておらず、安全な美白化粧品などへの応用は従来考
慮されていないという問題がある。本発明はこのような
問題に対処するためになされたもので、皮膚に適用して
安全であるとともに、美白作用を有する化粧品原料およ
びそれを用いた化粧品を提供することを目的とする。However, the extract of rooibos tea leaves is not known as an anti-inflammatory, antioxidant, or active oxygen-suppressing effect as a cosmetic raw material, and is a safe whitening cosmetic. However, there is a problem that the application to such as has not been considered conventionally. The present invention has been made to address such a problem, and it is an object of the present invention to provide a cosmetic raw material which is safe when applied to the skin and has a whitening effect, and a cosmetic using the same.
【０００４】[0004]
【課題を解決するための手段】本発明者らは、すでに多
年にわたって食用や飲用に供され、人体に対する安全性
が確認されている植物をスクリーニングして調べ、化粧
品原料および化粧品として利用価値のあるものをスクリ
ーニングして、その抗炎症性や抗酸化性等を検討した。
その結果、ルイボス茶葉の抽出物が化粧品原料としての
有効性を有することを見出した。特に抗炎症性や抗酸化
性に優れていることが確認され、本発明を完成するに至
った。Means for Solving the Problems The present inventors have screened and examined plants that have been used for food and drink for many years and have been confirmed to be safe for the human body, and are valuable as cosmetic raw materials and cosmetics. Those were screened to examine their anti-inflammatory properties and antioxidant properties.
As a result, they have found that the extract of rooibos tea leaves is effective as a raw material for cosmetics. In particular, it was confirmed that they had excellent anti-inflammatory properties and antioxidant properties, and the present invention was completed.
【０００５】すなわち、本発明の化粧品原料はルイボス
茶葉の抽出物からなる化粧品原料であって、該抽出物
は、ルイボス茶葉を水および親水性有機溶媒から選ばれ
た少なくとも一つの溶媒で抽出してなることを特徴とす
る。That is, the cosmetic raw material of the present invention is a cosmetic raw material comprising an extract of rooibos tea leaves, which is obtained by extracting rooibos tea leaves with at least one solvent selected from water and a hydrophilic organic solvent. It is characterized by becoming.
【０００６】また、特に水または含水有機溶媒からなる
溶媒で抽出された抽出物であることを特徴とする。[0006] It is also characterized in that it is an extract extracted with a solvent consisting of water or a water-containing organic solvent.
【０００７】本発明の化粧品は、上記化粧品原料を含有
することを特徴とする。The cosmetic of the present invention is characterized by containing the above-mentioned cosmetic raw materials.
【０００８】本発明の化粧品原料およびそれを用いた化
粧品は、特に抗炎症性や抗酸化性に優れているので、皮
膚に適用して安全であるとともに、美白作用を有する化
粧品となる。[0008] The cosmetic raw material of the present invention and cosmetics using the same are particularly excellent in anti-inflammatory properties and antioxidant properties, so that they can be applied to the skin and are safe and have a whitening effect.
【０００９】[0009]
【発明の実施の形態】本発明の化粧品原料は、学名アス
パラサス・リネアリス（Aspalathus linealis ）または
アスパラサス・セダルベルゲンシス（Aspalathus cedar
bergensis ）の葉を乾燥させ、あるいはその葉を細断し
て煤煎させたルイボス茶葉を抽出して得られる。本発明
に使用できるルイボス茶葉は、非煤煎あるいは煤煎させ
た茶葉であれば使用することができる。好ましいルイボ
ス茶葉は、煤煎させた茶葉であり、その条件は、特に限
定されないが、好ましくは煤煎温度が 120〜 150℃、煤
煎時間が10 〜 30 分である。煤煎させた茶葉は、有効
成分抽出の効率性から好ましい。BEST MODE FOR CARRYING OUT THE INVENTION The cosmetic raw material of the present invention has a scientific name of Aspalathus linealis or Aspalathus cedarbergensis (Aspalathus cedar).
bergensis) is obtained by drying the leaves or extracting the roasted rooibos leaves by chopping the leaves. Rooibos tea leaves that can be used in the present invention can be any non-sooted or sooted tea leaves. Preferred rooibos tea leaves are sooted tea leaves, the conditions of which are not particularly limited. Preferably, the sooting temperature is 120 to 150 ° C and the sooting time is 10 to 30 minutes. Sooted tea leaves are preferred from the viewpoint of the efficiency of extracting the active ingredient.
【００１０】ルイボス茶葉の抽出は、水あるいは親水性
有機溶媒、またはこれらの混合物を用いて行うことがで
きる。本発明に係る親水性有機溶媒は、水との親和性を
有する溶媒であり、たとえば、分子内に水酸基、カルボ
キシル基、カルボニル基などの親水基を有する有機溶媒
をいう。たとえば、エタノール、プロパノール、ブタノ
ール、エチレングリコール、プロピレングリコール、1,
3-ブチレングリコール、グリセリン、アセトン等を挙げ
ることができる。これらの中でも、化粧品原料としての
安全性を考慮して水、エタノールあるいはこれの混合溶
媒、あるいは、グリセリン、1,3-ブチレングリコール、
プロピレングリコール等の多価アルコール類、または多
価アルコール類と水との混合溶媒による抽出が好まし
い。The extraction of rooibos tea leaves can be carried out using water, a hydrophilic organic solvent, or a mixture thereof. The hydrophilic organic solvent according to the present invention is a solvent having an affinity for water, for example, an organic solvent having a hydrophilic group such as a hydroxyl group, a carboxyl group, and a carbonyl group in a molecule. For example, ethanol, propanol, butanol, ethylene glycol, propylene glycol, 1,
Examples thereof include 3-butylene glycol, glycerin, and acetone. Among them, water, ethanol or a mixed solvent thereof, or glycerin, 1,3-butylene glycol, in consideration of safety as a cosmetic raw material,
Extraction with a polyhydric alcohol such as propylene glycol or a mixed solvent of a polyhydric alcohol and water is preferred.
【００１１】本発明に係るルイボス茶葉の抽出にあたっ
て、特に水あるいは、水とエタノールとの混合溶媒が好
ましく、水単独の場合、熱水が特に好ましい。熱水抽出
の場合、後述するように、抗酸化性や抗炎症性に優れて
いる。In the extraction of rooibos tea leaves according to the present invention, water or a mixed solvent of water and ethanol is particularly preferable, and when water alone is used, hot water is particularly preferable. In the case of hot water extraction, as described later, it has excellent antioxidant properties and anti-inflammatory properties.
【００１２】抽出物は化粧品原料として利用されるが、
配合される化粧品に応じて抽出液のままでも、あるいは
凍結乾燥して粉体として利用することもできる。The extract is used as a raw material for cosmetics,
The extract can be used as it is or as a powder after freeze-drying depending on the cosmetics to be blended.
【００１３】たとえば、抽出物を他の化粧品原料、たと
えばスクワラン、ホホバ油等の液状油、ミツロウ、セチ
ルアルコール等の固体油、各種の活性剤、グリセリン、
1,3-ブチレングリコール等の保湿剤や各種薬剤等ととも
に配合してさまざまな剤形の化粧品を調整することがで
きる。たとえばローション、クリーム、乳液、パック等
で目的に応じた利用形態とすることができる。[0013] For example, the extract is used as a raw material for other cosmetics such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, glycerin,
It can be mixed with humectants such as 1,3-butylene glycol and various chemicals to prepare cosmetics of various dosage forms. For example, lotions, creams, emulsions, packs and the like can be used according to the purpose.
【００１４】[0014]
実施例１ 化粧品原料をつぎの方法で調整した。乾燥させたアスパ
ラサス・リネアリスの葉を煤煎温度が 120〜 150℃、煤
煎時間が 10 〜 30 分で煤煎させたルイボス茶葉を得
た。このルイボス茶葉 10 g に水 500mlを加えて時々攪
拌しつつ 3時間沸騰水浴上で加熱後、室温に冷却した。
これを濾過した後、凍結乾燥して粉体としての化粧品原
料を得た。Example 1 A cosmetic raw material was prepared by the following method. Rooibos tea leaves were obtained by soaking the dried leaves of Asparasas lineriais at a soaking temperature of 120 to 150 ° C and a soaking time of 10 to 30 minutes. 500 g of water was added to 10 g of the rooibos tea leaves, and the mixture was heated on a boiling water bath for 3 hours with occasional stirring, and then cooled to room temperature.
This was filtered and freeze-dried to obtain a cosmetic raw material as a powder.
【００１５】実施例２ 実施例１で得られたルイボス茶葉 10 g に 50 重量％エ
タノール水溶液 500mlを加えて時々攪拌しつつ 5日間室
温に放置した。これを濾過、エバポレートした後、凍結
乾燥して粉体としての化粧品原料を得た。Example 2 To 10 g of rooibos tea leaves obtained in Example 1, 500 ml of a 50% by weight aqueous ethanol solution was added, and the mixture was left at room temperature for 5 days with occasional stirring. This was filtered, evaporated, and freeze-dried to obtain a cosmetic raw material as a powder.
【００１６】実施例３ 実施例１で得られたルイボス茶葉 10 g にエタノール 5
00mlを加えて時々攪拌しつつ 5日間室温に放置した。こ
れを濾過、エバポレートした後、凍結乾燥して粉体とし
ての化粧品原料を得た。Example 3 10 g of rooibos tea leaves obtained in Example 1 was added to ethanol 5
After adding 00 ml, the mixture was left at room temperature for 5 days with occasional stirring. This was filtered, evaporated, and freeze-dried to obtain a cosmetic raw material as a powder.
【００１７】得られた化粧品原料をつぎの方法で評価し
た。評価方法と結果について説明する。The obtained cosmetic raw materials were evaluated by the following methods. The evaluation method and the result will be described.
【００１８】１．プロスタグランジンＥ2 生成抑制効果
試験 正常人皮膚線維芽細胞を 48 ウェルセルプレートに分植
し、コンフェルントになるまで培養した。培地は、牛胎
児血清を 10 ％含む Eagle'sMEM 培地を用い、37 ℃、
5% 炭酸ガス雰囲気下で培養した。培地を牛アルブミン
を 0.1% 含む Eagle'sMEM 培地に交換し、さらに 1晩培
養した。培養液を除去後、試験品を含む牛アルブミンを
0.1% 含む Eagle'sMEM 培地を 1ウェルにつき 0.8ml加
え、インターロイキン１−βを 5ng/ml になるように添
加し、さらに 1晩培養した。培養液中に遊離したプロス
タグランジンＥ2 は酵素免疫測定法にて測定した。ま
た、試験品の細胞に対する影響を知るためにパラニトロ
フェニールリン酸にて酸性フォスタファーゼ活性を測定
した。検体濃度とその評価結果を表１に示す。1. Prostaglandin E 2 production inhibitory effect test Normal human skin fibroblasts were seeded in a 48-well cell plate and cultured until they became confluent. Use Eagle's MEM medium containing 10% fetal calf serum at 37 ° C,
The cells were cultured in a 5% carbon dioxide atmosphere. The medium was replaced with Eagle's MEM medium containing 0.1% bovine albumin, and the cells were further cultured overnight. After removing the culture solution, add bovine albumin containing the test article.
0.8 ml of Eagle's MEM medium containing 0.1% was added per well, interleukin 1-β was added to a concentration of 5 ng / ml, and the cells were further cultured overnight. Prostaglandin E 2 released into the culture solution was measured by enzyme immunoassay. In addition, the acid phosphatase activity was measured with paranitrophenyl phosphate to determine the effect of the test article on cells. Table 1 shows the sample concentrations and the evaluation results.
【００１９】[0019]
【表１】 [Table 1]
【００２０】表１に示すように、本発明の化粧品原料は
プロスタグランジンＥ2 生成抑制効果が認められ、特に
実施例１および実施例２の化粧品原料がより優れた効果
を示した。なお、酸性フォスタファーゼ活性による細胞
に対する影響は、実施例１および実施例２の化粧品原料
が、実施例３の化粧品原料より少なく細胞に対する影響
が少なかった。As shown in Table 1, the cosmetic raw materials of the present invention exhibited an effect of suppressing the production of prostaglandin E 2 , and the cosmetic raw materials of Examples 1 and 2 showed more excellent effects. The effects of acidic phosphatase activity on cells were smaller for the cosmetic raw materials of Examples 1 and 2 than for the cosmetic raw materials of Example 3.
【００２１】２．抗炎症試験 プロスタグランジンＥ2 生成抑制効果に優れている実施
例１および実施例２の化粧品原料につき、さらにつぎの
抗炎症試験を行った。 １） Wistar 系ラット、雄、 5週齢（ＳＬＣ（株）） 5
匹を 1群として用いた。 ２）試験品の調整と適用 イ）検体群 ％実施例（凍結乾燥品）生理食塩水液 1ml/100g body w
eight を動物に皮下投与した。 ロ）対照群 生理食塩水液 1ml/100g body weight を動物に皮下投与
した。 ハ）起炎物質 カラゲニン（東京化成（株））を生理食塩水を用いて
0.5% 生理食塩水液として 0.1mlを右後足 へ皮下投与
した。３）試験方法 浮腫の測定は Winter らの方法に準じて容積法で行っ
た。水位の変化は、ＭＫ−５００デジタルボリュームメ
ータ（室町化学（株））により導電度の変化で測定し
た。すなわち、 イ）ラットの体重を測定してグループ分けした。 ロ）ラットの右後肢に浸漬部位を示す印をつけた。 ハ）その印の所まで水中に浸漬して排積される水の容積
を測定した。 ニ）起炎物質投与の 30 分前にそれそれ対照液および被
検液を皮下投与した。 ホ）ラットの右後肢足 へ起炎物質を皮下投与して浮腫
を誘発させた。 ヘ）起炎物質投与後 1時間ごとに 5時間目までラット右
後肢の容積の変化を測定して、つぎの式により浮腫率を
算出した。 浮腫率＝（Ａ−Ｂ）／Ｂ ここで、Ａはカラゲニン注射後の足容積、Ｂはカラゲニ
ン注射前の足容積である。経過時間とその評価結果を表
２に示す。2. Anti-inflammatory test The following anti-inflammatory test was further performed on the cosmetic raw materials of Examples 1 and 2 which were excellent in the prostaglandin E 2 production inhibitory effect. 1) Wistar rat, male, 5 weeks old (SLC) 5
The animals were used as one group. 2) Preparation and application of test product a) Sample group% Example (lyophilized product) Physiological saline solution 1ml / 100g body w
eight were administered subcutaneously to the animals. B) Control group Animals were subcutaneously administered 1 ml / 100 g body weight of physiological saline solution. C) Inflammatory substance Carrageenin (Tokyo Kasei Co., Ltd.) using physiological saline
0.1 ml of a 0.5% saline solution was subcutaneously administered to the right hind paw. 3) Test method Edema was measured by the volumetric method according to the method of Winter et al. The change in water level was measured by a change in conductivity using an MK-500 digital volume meter (Muromachi Chemical Co., Ltd.). A) Rats were weighed and grouped. B) A mark indicating the immersion site was marked on the right hind leg of the rat. C) The volume of water that was immersed in water up to the mark and discharged was measured. D) The control solution and the test solution were subcutaneously administered 30 minutes before the administration of the inflammatory substance. E) Edema was induced by subcutaneous administration of an inflammatory substance to the right hind paw of the rat. F) Every hour after the administration of the inflammatory substance, the change in the volume of the right hind paw of the rat was measured up to the fifth hour, and the edema rate was calculated by the following formula. Edema rate = (AB) / B where A is the paw volume after carrageenan injection, and B is the paw volume before carrageenin injection. Table 2 shows the elapsed time and the evaluation results.
【００２２】[0022]
【表２】 [Table 2]
【００２３】表２に示すように、本発明の化粧品原料は
抗炎症に優れ、化粧品原料としての安全性に優れてい
た。 ３．抗酸化試験 以下の試験液をネジキャップ付 50ml 試験管に作成す
る。 検体 5mg 2％リノール酸エタノール溶液 10ml 0.1M、pH7.0 リン酸緩衝液 10ml 精製水 5ml これを 50 ℃の恒温槽に遮光して放置する。恒温槽に入
れる前、 3日後、 7日後、 9日後、14日後に以下の測定
する。試験液 0.150mlと、反応液（ 75 ％エタノール 5
00mlと 30 ％チオシアン酸アンモニウム水溶液 5.15ml
との混合液） 14.7ml とを混合してタッチミキサーでよ
く攪拌し 3分間放置後、0.02M 塩化第一鉄 3.5％ HCl水
溶液0.150ml を加えてタッチミキサーでよく攪拌し 3分
間放置後、波長 500nmで吸光度を測定する。セル長 10n
m 、リファレンスは反応液である。測定結果を表３に示
す。なお、表３において、０日後は恒温槽に入れる前を
表す。As shown in Table 2, the cosmetic raw material of the present invention was excellent in anti-inflammatory and excellent in safety as a cosmetic raw material. 3. Antioxidant test Prepare the following test solution in a 50 ml test tube with a screw cap. Specimen 5 mg 2% linoleic acid ethanol solution 10 ml 0.1 M, pH 7.0 Phosphate buffer 10 ml Purified water 5 ml Leave this in a 50 ° C constant temperature bath, protected from light. Before placing in a thermostat, after 3, 7, 9, and 14 days, measure below. 0.150 ml of test solution and reaction solution (75% ethanol 5
00ml and 30% ammonium thiocyanate aqueous solution 5.15ml
14.7 ml), mix well with a touch mixer, leave for 3 minutes, add 0.150 ml of 0.02M ferrous chloride 3.5% HCl aqueous solution, stir well with a touch mixer, leave for 3 minutes, and Measure absorbance at 500 nm. Cell length 10n
m, reference is the reaction solution. Table 3 shows the measurement results. In addition, in Table 3, after 0 days, it represents before putting in a thermostat.
【００２４】[0024]
【表３】 [Table 3]
【００２５】表３に示すように、本発明の化粧品原料
は、抗酸化能に優れたビタミンＥよりも優れた抗酸化能
を示した。また、実施例１および実施例２の化粧品原料
が、実施例３の化粧品原料に比較して、優れた抗酸化能
を示した。As shown in Table 3, the cosmetic raw material of the present invention exhibited better antioxidant activity than vitamin E, which had excellent antioxidant activity. In addition, the cosmetic raw materials of Example 1 and Example 2 exhibited excellent antioxidant ability as compared with the cosmetic raw material of Example 3.
【００２６】４．紫外線紅斑抑制試験 本発明に係る化粧品原料の紫外線によって誘発される紫
外線紅斑抑制効果を 8週齢の雄性白色モルモット 6匹に
よりつぎの方法により試験した。 １）試験方法 1試験につき、 3試験試料の評価を実施する。脱毛した
モルモット背部に脊中対称に 3部位づつ、計６箇所にソ
ーラーシュミレーター（601 型：SOLAR LIGHT 社）にて
UVA および UVBを2.O MED/cm2 照射する。検体照射直
後、パッチ伴（φ＝ 1.5cm：鳥居薬品）に試験試料およ
び対照としての溶媒を 0.1mlづつしみこませ、左右対称
に 2時間閉塞貼布する。判定は照射終了後、 1、 2、 3
および 4時間目に下記基準に基づき肉眼で判定する。薬
効の評価は試験試料側と溶媒側の平均スコアを比較する
ことにより行う。判定基準としては、各モルモットにつ
き、紅斑なしを０、わずかな紅斑を１、境界不明瞭な紅
斑を２、境界明瞭な紅斑を３とし、加重平均値を求め
る。評価結果を表４に示す。4. UV erythema suppression test The cosmetic material of the present invention was tested for its UV erythema suppression effect induced by ultraviolet rays using six 8-week-old male white guinea pigs by the following method. 1) Test method For each test, evaluate three test samples. Three vertebrae symmetrically on the back of the guinea pig with hair loss, using a solar simulator (type 601: SOLAR LIGHT) at a total of six locations
Irradiate UVA and UVB 2.O MED / cm 2 . Immediately after sample irradiation, soak 0.1 ml of the test sample and solvent as a control into the patch (φ = 1.5 cm: Torii Pharmaceutical Co., Ltd.), and apply symmetrical closure for 2 hours. Judgment is made after irradiation, 1, 2, 3
And at the 4th hour, judge visually with the following criteria. The evaluation of drug efficacy is performed by comparing the average scores of the test sample side and the solvent side. As a criterion, a weighted average value is determined for each guinea pig, with no erythema being 0, slight erythema being 1, erythema with poorly defined boundaries being 2 and erythema with clearly defined boundaries being 3. Table 4 shows the evaluation results.
【００２７】[0027]
【表４】 [Table 4]
【００２８】表４に示すように、実施例１および実施例
２の化粧品原料は、対照に比較して優れた紫外線紅斑抑
制効果を有している。As shown in Table 4, the cosmetic raw materials of Examples 1 and 2 have an excellent ultraviolet erythema inhibitory effect as compared with the control.
【００２９】５．活性酸素抑制効果試験 pH8.2 緩衝液（リン酸二水素ナトリウム 65mM 、ホウ酸
ソーダ 35mM 、エチレンジアミン四酢酸二ナトリウム
0.5mM）を 0.2ml、 0.5mMキサンチンを 0.2ml、1mMヒド
ロキシルアミン塩酸塩水溶液 0.1ml、水 0.1mlをバイヤ
ル瓶に加えて攪拌し、検体 0.1mlを加えてさらに水 0.1
mlを加えて攪拌し、キサンチンオキシダーゼ水溶液（ミ
ルク製、 6unit／ml） 0.2mlを加えて、 37 ℃で 30 分
間放置した。これに発色液を 2.Oml加えて室温で放置後
30 分〜180 分の間に 550nmの吸光度を測定した。な
お、検体の代わりに水を測定し、ブランクとした。活性
酸素抑制効果は、キサンチンから尿酸への酸化を阻害す
る阻害率として表した。評価結果を表５に示す。5. Active oxygen suppression effect test pH8.2 buffer solution (sodium dihydrogen phosphate 65mM, sodium borate 35mM, disodium ethylenediaminetetraacetate)
0.2 ml of 0.5 mM xanthine, 0.2 ml of 0.5 mM xanthine, 0.1 ml of 1 mM aqueous solution of hydroxylamine hydrochloride and 0.1 ml of water are added to a vial, and the mixture is stirred.
Then, 0.2 ml of an aqueous solution of xanthine oxidase (made of milk, 6 unit / ml) was added, and the mixture was allowed to stand at 37 ° C. for 30 minutes. Add 2.Oml of coloring solution to this and leave it at room temperature
The absorbance at 550 nm was measured between 30 minutes and 180 minutes. In addition, water was measured instead of the sample, and was used as a blank. The active oxygen suppression effect was expressed as an inhibition rate that inhibits oxidation of xanthine to uric acid. Table 5 shows the evaluation results.
【００３０】[0030]
【表５】 [Table 5]
【００３１】表５に示すように、本発明の化粧品原料は
活性酸素の抑制に優れた効果を示し、特に実施例１およ
び実施例２の化粧品原料がより優れた効果を示した。As shown in Table 5, the cosmetic raw materials of the present invention exhibited an excellent effect of suppressing active oxygen, and the cosmetic raw materials of Examples 1 and 2 showed more excellent effects.
【００３２】以上のプロスタグランジンＥ2 生成抑制効
果試験、抗炎症試験、抗酸化試験、紫外線紅斑抑制試
験、活性酸素抑制効果試験より、本発明の化粧品原料
は、皮膚に対して安全であり、かつ美白作用を有する物
質であることがわかった。From the prostaglandin E 2 production inhibitory effect test, anti-inflammatory test, antioxidant test, ultraviolet erythema inhibitory test and active oxygen inhibitory effect test, the cosmetic raw material of the present invention is safe for the skin, It was also found to be a substance having a whitening effect.
【００３３】実施例４ 実施例１で得られた化粧品原料を用いて化粧品としての
ローションを得た。配合成分と配合量（重量部）を以下
に示す。 オリーブ油 ０．５ 実施例１の化粧品原料 ０．５ ポリオキシエチレン(20E.0) ソルビタンモノステアレート ２．０ ポリオキシエチレン(60E.0) 硬化ヒマシ油 ２．０ エタノ一ル １０．０ 1.0 重量％ヒアルロン酸ナトリウム水溶液 ５．０ 精製水 ８０．０Example 4 A cosmetic lotion was obtained using the cosmetic raw material obtained in Example 1. The components and amounts (parts by weight) are shown below. Olive oil 0.5 Cosmetic raw material of Example 1 0.5 Polyoxyethylene (20E.0) sorbitan monostearate 2.0 Polyoxyethylene (60E.0) Hardened castor oil 2.0 Ethanol 10.0 1.0 weight % Aqueous sodium hyaluronate 5.0 Purified water 80.0
【００３４】精製水にヒアルロン酸ナトリウム水溶液加
え 70 ℃に加熱調整する。オリーブ油にポリオキシエチ
レン（ 20 E.0 ）ソルビタンモノステアレートおよびポ
リオキシエチレン（ 60 E.0 ）硬化ヒマシ油を加え 70
℃に加熱調整する。この油相を先に調整した水相に加え
予備乳化し、さらに実施例１の化粧品原料およびエタノ
ールを加えてホモミキサーにて乳化粒子を均一にした
後、脱気、濾過、冷却して実施例４のローションを得
た。An aqueous solution of sodium hyaluronate is added to purified water, and the temperature is adjusted to 70 ° C. by heating. Add polyoxyethylene (20E.0) sorbitan monostearate and polyoxyethylene (60E.0) hydrogenated castor oil to olive oil.
Heat to ℃. This oil phase was added to the previously prepared aqueous phase and pre-emulsified. Further, the cosmetic raw material of Example 1 and ethanol were added to homogenize the emulsified particles with a homomixer, followed by degassing, filtration, and cooling. 4 lotions were obtained.
【００３５】実施例５ 実施例２で得られた化粧品原料を用いて化粧品としての
クリームを得た。配合成分と配合量（重量部）を以下に
示す。 Ａ成分 スクワラン ２０．０ オリーブ油 ２．０ ミンク油 １．０ ホホバ油 ５．０ ミツロウ ５．０ セトステアリルアルコール ２．０ グリセリンモノステアレート １．０ ソルビタンモノステアレート ２．０ 実施例２の化粧品原料 １．０ Ｂ成分 精製水 ４７．９ ポリオキシエチレン(20E.0) ソルビタンモノステアレート ２．０ ポリオキシエチレン(60E.0) 硬化ヒマシ油 １．０ グリセリン ５．０ 1.0 重量％ヒアルロン酸ナトリウム水溶液 ５．０ パラオキシ安息香酸メチル ０．１ Ａ成分とＢ成分とをそれぞれ計量し、それぞれ 70 ℃ま
で加温し、Ｂ成分にＡ成分を攪拌しつつ徐々に加えたの
ち、ゆっくり攪拌しつつ 30 ℃まで冷却して実施例５の
クリームを得た。Example 5 A cosmetic cream was obtained using the cosmetic raw material obtained in Example 2. The components and amounts (parts by weight) are shown below. A ingredient Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Cosmetic raw material of Example 2 1.0 Component B Purified water 47.9 Polyoxyethylene (20E.0) Sorbitan monostearate 2.0 Polyoxyethylene (60E.0) Hardened castor oil 1.0 Glycerin 5.0 1.0% by weight aqueous sodium hyaluronate solution 5.0 Methyl paraoxybenzoate 0.1 A component and B component are each weighed and heated to 70 ° C., respectively, and A component is gradually added to B component with stirring, and then 30 ° C. with slow stirring. After cooling, the cream of Example 5 was obtained.
【００３６】実施例６ 実施例１の化粧品原料を実施例３の化粧品原料に代える
以外は実施例４と同一の配合処方でローションを得た。Example 6 A lotion was obtained with the same formulation as in Example 4, except that the cosmetic raw material of Example 1 was replaced with the cosmetic raw material of Example 3.
【００３７】実施例４から実施例６で得られた化粧品を
用いて以下の使用テストを行った。女性 6名づつの顔面
を左右に分け、片側の顔面を実施例、もう片側の顔面を
比較例として毎日、実施例のローションおよびクリーム
を 1回以上使用してもらって 3月後、使用後の肌荒れ防
止や肌のつや、肌のはりについてアンケートした。比較
例１は実施例４より化粧品原料を水に代えたローション
であり、比較例２は実施例５より化粧品原料を水に代え
たクリームである。なお、 12 名を 2班に分け表６に示
す試料を用いて実験した。また、判定基準を以下に示
す。アンケートの結果をまとめて表６に示す。判定基準
は以下に示す点数で表し、その結果を集計した。 実施例が比較例より非常によい ３ 実施例が比較例よりかなりよい ２ 実施例が比較例よりややよい １ 実施例と比較例と差がない ０ 比較例が実施例よりややよい −１ 比較例が実施例よりかなりよい −２ 比較例が実施例より非常によい −３The following use tests were carried out using the cosmetics obtained in Examples 4 to 6. The face of each of six women was divided into left and right, and the face on one side was used as an example, and the face on the other side was used as a comparative example. The questionnaire was asked about prevention, skin gloss, and skin scalp. Comparative Example 1 is a lotion in which the cosmetic raw material was replaced with water from Example 4, and Comparative Example 2 was a cream in which the cosmetic raw material was replaced with water from Example 5. In addition, 12 persons were divided into two groups and experiments were performed using the samples shown in Table 6. The criteria are shown below. Table 6 summarizes the results of the questionnaire. The judgment criteria were represented by the following points, and the results were totaled. Example is much better than Comparative Example 3 Example is considerably better than Comparative Example 2 Example is slightly better than Comparative Example 1 No difference between Example and Comparative Example 0 Comparative Example is slightly better than Example -1 Comparative Example Is much better than the example-2. The comparative example is much better than the example-3.
【００３８】[0038]
【表６】 [Table 6]
【００３９】表６に示すように、本発明の化粧品は、肌
荒れ防止や、肌のつや、肌のはりに顕著な効果が認めら
れた。As shown in Table 6, the cosmetics of the present invention were found to have remarkable effects on the prevention of rough skin, the luster of the skin, and the peeling of the skin.
【００４０】[0040]
【発明の効果】本発明の化粧品原料は、ルイボス茶葉を
水および親水性有機溶媒から選ばれた少なくとも一つの
溶媒で抽出してなるので、優れた抗炎症および抗酸化性
を有する。The cosmetic raw material of the present invention is obtained by extracting rooibos tea leaves with at least one solvent selected from water and a hydrophilic organic solvent, and thus has excellent anti-inflammatory and antioxidant properties.
【００４１】また、水または含水有機溶媒からなる溶媒
で抽出してなるので、より優れた抗炎症および抗酸化作
用を有する。Further, since it is extracted with a solvent composed of water or a water-containing organic solvent, it has better anti-inflammatory and anti-oxidative effects.
【００４２】本発明の化粧品は、上述の化粧品原料を含
むので、皮膚に安全で美白作用があり、肌荒れ防止や、
肌のつや、肌のはりに非常に良好な効果をもたらす。Since the cosmetic of the present invention contains the above-mentioned cosmetic raw materials, it has a safe and whitening effect on the skin, prevents rough skin,
It has a very good effect on skin shine and skin radiance.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 阪井 知香 三重県松阪市久保町字太田６−５ (72)発明者 大森 正樹 愛知県東海市荒尾町向山21−２−310 ──────────────────────────────────────────────────続 き Continued on the front page (72) Chika Sakai, Inventor 6-5 Ota, Kubo-cho, Matsusaka-shi, Mie (72) Masaki Omori 21-2-310, Mukaiyama, Arao-cho, Tokai-shi, Aichi
Claims (3)
料であって、前記抽出物は、前記ルイボス茶葉を水およ
び親水性有機溶媒から選ばれた少なくとも一つの溶媒で
抽出してなることを特徴とする化粧品原料。1. A cosmetic material comprising an extract of rooibos tea leaves, wherein the extract is obtained by extracting the rooibos tea leaves with at least one solvent selected from water and a hydrophilic organic solvent. Cosmetic ingredients.
ることを特徴とする請求項１記載の化粧品原料。2. The cosmetic raw material according to claim 1, wherein the solvent is water or a water-containing organic solvent.
料を含有する化粧品。3. A cosmetic comprising the cosmetic raw material according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9278451A JPH11116429A (en) | 1997-10-13 | 1997-10-13 | Cosmetic stock and cosmetic |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9278451A JPH11116429A (en) | 1997-10-13 | 1997-10-13 | Cosmetic stock and cosmetic |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH11116429A true JPH11116429A (en) | 1999-04-27 |
Family
ID=17597531
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9278451A Pending JPH11116429A (en) | 1997-10-13 | 1997-10-13 | Cosmetic stock and cosmetic |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH11116429A (en) |
Cited By (11)
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|---|---|---|---|---|
| KR20010044746A (en) * | 2001-03-21 | 2001-06-05 | 진형근 | Moisturizing Rooibos for Skin Care |
| KR100379301B1 (en) * | 2000-09-28 | 2003-04-08 | 주식회사 도도화장품 | Method of extracting a valuable component from the rooibos, methods of producing a skin lotion, a milk lotion and an emolint cream using the same |
| JP2005132822A (en) * | 2003-08-21 | 2005-05-26 | Neutrogena Corp | Stabilized composition containing oxygen unstable active agent |
| JP2005170805A (en) * | 2003-12-08 | 2005-06-30 | Chanson Keshohin Kk | Skin care cosmetic, cosmetic set thereof and method for using them |
| KR100510104B1 (en) * | 2001-12-29 | 2005-08-24 | 주식회사 코리아나화장품 | Fermentative Extract of Rooibos and Cosmetic Compositions Comprising the Same for Preventing Skin Aging |
| KR100538536B1 (en) * | 2002-11-22 | 2005-12-22 | 주식회사 다사랑 | The manufacturing method of the beauty pack including salicornia herbaceea. |
| WO2006081989A1 (en) * | 2005-01-31 | 2006-08-10 | Raps Gmbh & Co. Kg | Rooibos extract with an increased aspalathin content, method for producing one such rooibos extract, and cosmetic agent containing the same |
| JP2010270152A (en) * | 2010-09-03 | 2010-12-02 | Toyo Hakko:Kk | Antiallergic composition and skin-lightening composition, and cosmetic and food and drink containing these |
| JP2011105644A (en) * | 2009-11-17 | 2011-06-02 | Maruzen Pharmaceut Co Ltd | Melanin uptake inhibitor |
| JP2014162787A (en) * | 2013-02-28 | 2014-09-08 | Ichimaru Pharcos Co Ltd | Kinesin inhibitor |
| EP2069334B2 (en) † | 2007-10-23 | 2014-11-05 | Kneipp GmbH | Aspalathin-like dihydrochalcone, extracts from unfermented rooibos and process for preparation |
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| KR100379301B1 (en) * | 2000-09-28 | 2003-04-08 | 주식회사 도도화장품 | Method of extracting a valuable component from the rooibos, methods of producing a skin lotion, a milk lotion and an emolint cream using the same |
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| KR100510104B1 (en) * | 2001-12-29 | 2005-08-24 | 주식회사 코리아나화장품 | Fermentative Extract of Rooibos and Cosmetic Compositions Comprising the Same for Preventing Skin Aging |
| KR100538536B1 (en) * | 2002-11-22 | 2005-12-22 | 주식회사 다사랑 | The manufacturing method of the beauty pack including salicornia herbaceea. |
| JP2005132822A (en) * | 2003-08-21 | 2005-05-26 | Neutrogena Corp | Stabilized composition containing oxygen unstable active agent |
| JP2005170805A (en) * | 2003-12-08 | 2005-06-30 | Chanson Keshohin Kk | Skin care cosmetic, cosmetic set thereof and method for using them |
| WO2006081989A1 (en) * | 2005-01-31 | 2006-08-10 | Raps Gmbh & Co. Kg | Rooibos extract with an increased aspalathin content, method for producing one such rooibos extract, and cosmetic agent containing the same |
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| EP2069334B2 (en) † | 2007-10-23 | 2014-11-05 | Kneipp GmbH | Aspalathin-like dihydrochalcone, extracts from unfermented rooibos and process for preparation |
| JP2011105644A (en) * | 2009-11-17 | 2011-06-02 | Maruzen Pharmaceut Co Ltd | Melanin uptake inhibitor |
| JP2010270152A (en) * | 2010-09-03 | 2010-12-02 | Toyo Hakko:Kk | Antiallergic composition and skin-lightening composition, and cosmetic and food and drink containing these |
| JP2014162787A (en) * | 2013-02-28 | 2014-09-08 | Ichimaru Pharcos Co Ltd | Kinesin inhibitor |
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