JP3235922B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a highly safe cosmetic which has a high whitening effect, inhibits the activity of hyaluronidase, and is effective for rough skin.

[0002]

BACKGROUND OF THE INVENTION Lagerstroemia sp.
eciosa) is a semi-deciduous tree that grows in India and is a plant belonging to the genus Lagerstroemia in the family Sorrelidae. The roots of this crab are used for diarrhea, and the bark and leaves are used as laxatives.

[0003] On the other hand, various substances having a whitening effect that can be used as a raw material of cosmetics are known, but synthetic products have no guarantee of safety when applied to human skin for a long time. , Use is being restricted. On the other hand, many natural products have a weak whitening effect. However, it is a natural product from the aspect of safety for human skin, and humans eat it for many years,
It is guaranteed in terms of safety, and has a strong whitening effect,
There is a need for a substance that also has other effects on the skin.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to provide a cosmetic composition which is safe when applied to the skin, has a large whitening effect, inhibits the activity of hyaluronidase, and further contains an effective ingredient for rough skin. To provide.

[0005]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have screened and examined plants which have been used for food for many years and which have been confirmed to be safe for the human body. We considered something worthwhile. As a result, the present inventors have found that P. persica is effective as a cosmetic raw material or as a quasi-drug, and completed the present invention. That is, the present invention relates to
Speciosa).

[0006]

The effects of the solvent extract of C. versicolor to be used as the cosmetic of the present invention are as follows: first, skin whitening effect; second, hyaluronidase activity inhibitory effect;
The fourth is an active oxygen suppressing action, and the fourth is an antioxidant action. The activity suppressing effect of the second hyaluronidase will be described in more detail. Hyaluronidase is an enzyme widely distributed in living organisms and also present on skin, and as its name implies, degrades hyaluronic acid. Hyaluronic acid is a linear high molecular polysaccharide in which β-DN-acetylglucosamine and β-D-glucuronic acid are alternately bonded, and is a glycosaminoglucan widely present in connective tissues of mammals together with chondroitin sulfate and the like. Is a kind of The action of hyaluronic acid in connective tissue is to maintain water in the intercellular space, form a jelly-like matrix in the tissue to hold the cells, maintain the lubricity and flexibility of the skin, Mechanical failure)
And prevent bacterial infection. In addition, it is said that hyaluronic acid in the skin decreases with age, which results in aging such as fine wrinkles and dryness. Therefore, suppressing the activity of the hyaluronidase that degrades this hyaluronic acid, the stability of the hyaluronic acid used in the formulation, the hyaluronic acid of the formulation after application to the skin and the hyaluronic acid present in the skin It is thought to contribute to stability.

[0007] The third active oxygen suppressing action will be described in more detail. In general, organisms (except anaerobic ones) cannot exist without oxygen in the air. But,
Oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes.
This active oxygen oxidizes fatty acids to form peroxides. Phospholipids in biological membranes of living organisms also oxidize and cause damage. In addition, it is said that the generated peroxide and active oxygen damage DNA and promote aging. This active oxygen affects the mechanism of producing melanin from tyrosine and is also involved in skin darkening. Suppressing this active oxygen is important for the skin, in other words, an important factor required for cosmetics.

[0008] As a method of utilizing the sedge beetle,
Extract with water or a hydrophilic organic solvent such as ethanol, methanol, acetone or the like. However, since it is an extraction of cosmetic raw materials, it is natural that extraction with water, ethanol, or a mixed solvent thereof is preferable. Also,
In some cases, a polyhydric alcohol such as glycerin, 1,3-butylene glycol, propylene glycol, or a mixture of polyhydric alcohol and water can also be used for extraction. Furthermore, it is also effective to freeze-dry and use as a powder depending on the method of use.

This substance is used as a raw material for other cosmetics, for example, liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin, 1,3-butylene glycol and various other oils. Various formulations of cosmetics, such as lotions, creams,
It can be prepared into cosmetics in various forms depending on the purpose, such as emulsions and packs.

[0010]

EXAMPLES Examples of the production and practical use of the extract of Prunus brassicae used in the present invention are described below, but the present invention is not limited to these production examples and examples. is not.

[Production Example 1] 300 ml of ethanol was added to 10 g of a leaf of Salvia versicolor (dry product), and the mixture was allowed to stand for 5 days with occasional stirring. This was lyophilized after filtration.

[Production Example 2] 300 ml of a 50% aqueous ethanol solution was added to 10 g of a leaf of Salvia versicolor (dry product), and the mixture was left for 5 days with occasional stirring. This was lyophilized after filtration.

[Production Example 3] 300 ml of purified water was added to 10 g of a leaf of Salvia versicolor (dry product), and the mixture was heated for 3 hours. This was allowed to cool, filtered, and lyophilized.

Example 1 (Preparation of lotion) The following components were mixed to prepare a lotion according to a conventional method. (% By weight) Olive oil 0.5 Ethanol extract of Prunus brassicae in Preparation Example 0.5 Polyoxyethylene (20E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60E.O.) hydrogenated castor oil 2 0.0 Ethanol 10.0 1.0% aqueous solution of sodium hyaluronate 5.0 Purified water 80.0

Example 2 (Preparation of a cream) A and B comprising the following components were each heated to 70 ° C., and then A was gradually added to B while stirring, and then slowly stirred. While cooling to 30 ° C., a cream was prepared. (% By weight) A Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan monostearate 2.0 Production example 2 50% Ethanol Extract of P. serrata in Japan 1.0B Purified Water 47.9 Polyoxyethylene (20E.O.) Sorbitan Monostearate 2.0 Polyoxyethylene (60E.O.) Hardened Castor Oil 1.0 Glycerin 5.0 1.0% aqueous solution of sodium hyaluronate 5.0 methyl methyl paraoxybenzoate 0.1

Example 3 (Preparation of lotion) The extract of Production Example 1 in Example 1 was prepared by changing the extract of Production Example 3.

[Inhibition of Tyrosinase Activity] (Test method) Mcllvaln buffer 0.9m
l, 1.0 ml of 1.66 mM tyrosine solution, 0.1 wt / v% aqueous solution of the above-mentioned preparation (freeze-dried product) (If it is difficult to dissolve, add ethanol and dissolve, then add purified water and evaporate. After removing ethanol, 0.1w
1.0 ml) was placed in a screw vial and heated in a constant temperature water bath at 37 ° C. for 5 minutes or more. Tyrosinase solution (manufactured by Sigma, from mushroom, 914
(Unit / ml) was added, and the solution was kept warm in a constant temperature water bath at 37 ° C., and the absorbance was measured at 475 nm after 10 minutes. As a control, pure water was added instead of the sample solution, and the measurement was performed in the same manner.
In this test, the final concentration of the sample is 0.033%. (Calculation formula) Tyrosinase activity inhibition rate (%) = {B- (AP)} /
B × 100 where A: absorbance of sample specimen B: absorbance of control P: absorbance due to coloring of sample specimen (3 times dilution)

[0018]

[Table 1]

[Hyaluronidase activity inhibition test] (Test method) 0.4% sodium hyaluronate 0.1M
(PH 6.0) After weighing out 6 g of a phosphate buffer solution and leaving it to stand in a constant temperature water bath at 37 ° C. for 5 minutes, a 0.1 wt / v% aqueous solution of the above-mentioned production example (freeze-dried product) was added. After adding and dissolving, purified water was added and evaporated, and ethanol was removed. Then, 1.0 ml of the mixture was adjusted to be 0.1 wt / v%), and the mixture was stirred and 0.01% hyaluronidase (Sigma cattle) Made of testicles, type IS)
1 ml of a 0.1 M (pH 6.0) phosphate buffer was added and the mixture was immediately stirred, and 6 ml of the mixture was placed in an Ostwald viscometer placed in a thermostat bath at 37 ° C. After 1 minute, 5 minutes, 10 minutes, 20 minutes, and 40 minutes, the viscosity was measured. As a control, pure water was added in place of the sample solution, and measurement was performed in the same manner. In this test, the final concentration of each sample is 0.0125% of the concentration of the sample. The results are shown in Tables 2 to 4 below as indices, with the viscosity after 1 minute as 100.

[0020]

[Table 2]

[0021]

[Table 3]

[0022]

[Table 4]

[Active Oxygen Suppression Test] There are various methods for measuring the effect of suppressing active oxygen, and the following method was used this time. pH7.8 50 mM potassium phosphate buffer (containing 1.3 mM DETAPAC) 133 ml 40 unit / ml above potassium phosphate buffer with catalase 5 ml 2 mM above potassium phosphate buffer with nitroblue tetrazolium 5 ml 1.8 mM xanthin above phosphorus Potassium acid buffer 17ml 160ml

2.4 ml of the above mixture of reagents and 0.3 ml of the specimen
In addition, a xanthine oxynase (water is used as a sample in advance, and when the experiment is performed, the absorbance is adjusted with the above potassium phosphate buffer so that the absorbance increases by about 0.02 per minute).
Immediately after adding 1 ml, measure the absorbance (560 nm).
Quantile and confirm linearity). (Calculation formula) Inhibition rate = {(AB) / A} × 100 where A: change in absorbance per minute when the sample is water B: change in absorbance per minute of sample Several steps were performed to find a 50% active oxygen production inhibiting concentration. A sample was prepared by preparing an aqueous solution of the above-mentioned production example (freeze-dried product) at an appropriate concentration (if it is difficult to dissolve, add ethanol to dissolve it, add purified water, evaporate, remove ethanol, and Concentration).

[0025]

[Table 5]

[Antioxidant test] 50 with the following screw cap
An ml test tube was prepared. Specimen 5 ml 2% linoleic acid ethanol solution 10 ml 0.1 M, pH 7.0 phosphate buffer 10 ml purified water 5 ml This 50 ml test tube with a screw cap is left in a constant temperature bath at 50 ° C. while being shielded from light. Before putting it in a thermostat, 4 days later, 7
The following measurements were taken after 11 days. Test solution 0.125
ml, 12.125 ml of 75% ethanol, and 0.125 ml of 30% ammonium thiocyanate.
0.125 ml of a 0.02 N ferrous chloride 3.5% HCl aqueous solution was added, and the mixture was stirred.
The absorbance was measured in nm. The cell length was 10 mm, and the control cell was one in which the test solution was replaced with water.

[0027]

[Table 6]

[0028]

[Table 7]

(Usage test) The face of six women was divided into left and right, and the lotion and cream of the example were set on one side, and the lotion and cream of the comparative example were set on the other side, and used at least once a day. After three months,
Evaluation was made on whitening, prevention of rough skin, luster of the skin, and skin swelling. In Comparative Examples, water extract was used in place of various extracts of Pseudomonas japonicus in Preparation Examples from Examples (Comparative Examples 1 and 2). In addition, 12 persons were divided into two groups and tested using the samples shown in Table 8 below.

[0030]

[Table 8]

The evaluation was performed according to the following evaluation criteria, and the results are summarized in Table 9 below. (Evaluation criteria) Example is very good 3 Example is considerably better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better − 2 Comparative example is much better -3

[0032]

[Table 9]

Results of the tyrosinase activity suppression test (Table 1), hyaluronidase activity suppression test results (Tables 2 to 4), active oxygen suppression test results (Table 5), antioxidant tests (Tables 6 and 7), use As is clear from the test (Table 9), the cosmetic containing the solvent extract of Streptomyces cerevisiae according to the present invention suppresses the activity of tyrosinase, the activity of hyaluronidase and active oxygen, and prevents whitening, rough skin, luster and skin. It turned out to be effective for the beam.

[0034]

According to the present invention, there is provided a highly safe cosmetic which has a high whitening effect, suppresses the activity of hyaluronidase, and is effective for rough skin.

──────────────────────────────────────────────────続 き Continuation of the front page (72) Inventor Kenji Shimomura 3-16-32 Funae, Ise City, Mie Prefecture (72) Inventor Masami Nakamura 6-32, Ikegami-cho, Toba City, Mie Prefecture (56) References JP-A-3-3 201969 (JP, A) JP-A-5-310587 (JP, A) JP-A-52-82711 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 7/00-7 / 50

Claims (1)

(57) [Claims] [Claim 1] Lagerstroemia sp.
cosmetics containing a solvent extract of eciosa).