JP3545057B2 - Cosmetics - Google Patents

Description

【００１５】

ＡとＢをそれぞれ計量し、７０℃まで加温し、ＢにＡを撹拌しつつ徐々に加えたのち、ゆっくり撹拌しつつ３０℃まで冷却した。
【００１６】
実施例３は実施例１の製造例１の抽出物を製造例３の抽出物に変え作成したもの。
【００１７】
（チロシナーゼ活性阻害試験）
（試験方法）
マックルバルン（Ｍｃｌｌｖａｌｎ）緩衝液０．９ｍｌ、１．６６ｍＭチロシン（Ｔｙｒｏｓｉｎｅ）溶液１．０ｍｌ、前記製造例（凍結乾燥品）の０．１ｗｔ／ｖ％水溶液（溶解しにくい場合はエタノールを加えて溶解したのち精製水を加えて、エバポレートし、エタノールを除去したのち、０．１ｗｔ／ｖ％になるように調製した）１．０ｍｌをスクリューバイアルにとり、３７℃恒温水槽中で５分以上加温した。
チロシナーゼ溶液（Ｓｉｇｍａ社製、マッシュルーム由来、９１４ユニット／ｍｌ）０．１ｍｌを加え、３７℃恒温水槽中で保温し、１０分後に４７５ｎｍで吸光度を測定した。
対照として、上記試料液のかわりに純水を加え同様に測定した。
この試験では試料の終濃度は０．０３３％となる。

その結果を表１に示す。
【００１８】
【表１】

【００１９】
（活性酸素抑制試験）
活性酸素を抑制する効果を測定する方法は各種あるが、今回以下の方法を利用した。

上の試薬の混合物を２．４ｍｌ、検体を０．３ｍｌ加えてキサンチンオキシナーゼ（予め検体を水とし、実験するとき、吸光度が１分当たり０．０２前後上昇するように上記のリン酸カリウム緩衝液で調整しておく）液を０．１ｍｌ加えて直ちに吸光度（５６０ｎｍ）を測定する。（測定は２分位し、直線性を確認する）
計算式 阻害率＝（（Ａ−Ｂ）／Ａ）×１００
Ａ：検体を水としたときの１分当たりの吸光度の変化
Ｂ：検体の１分当たりの吸光度の変化
濃度段階を数段階行い、５０％活性酸素生成阻害濃度を探した。検体の作成方法は前記製造例（凍結乾燥品）を適当な濃度の水溶液（溶解しにくい場合はエタノールを加えて溶解したのち精製水を加えて、エバポレートし、エタノールを除去したのち適当な濃度％となるように調製した）とした。
製造例２についての、５０％活性酸素生成阻害濃度の結果を表２に示す。
【００２０】
【表２】

【００２１】
（抗酸化試験）
以下の試験液をネジキャップ付５０ｍｌ試験管に作成した。
検体 ５ｍｇ
２％リノール酸エタノール溶液 １０ｍｌ
０．１Ｍ，ｐＨ７．０リン酸緩衝液 １０ｍｌ
精製水 ５ｍｌ
これを５０℃の恒温槽に遮光して放置する。
これを恒温槽に入れる前と数日間隔で下記の測定をした。
試験液０．１２５ｍｌ、７５％エタノール１２．１２５ｍｌ、３０％チオシアン酸アンモニウム０．１２５ｍｌを加えて撹拌し３分間放置後、０．０２Ｎ塩化第一鉄３．５％ＨＣｌ水溶液０．１２５ｍｌを加えて撹拌し３分間放置後波長５００ｎｍで吸光度を測定した。セル長１０ｍｍ、対照セルは試験液を水に置き換えたもの。
その結果を表３、表４に示す。
【００２２】
【表３】

【００２３】
【表４】

【００２４】
（使用テスト）
女性６名づつの顔面を左右に分け、一方を実施例、もう一方を比較例として毎日、１回以上使用してもらって、３月後、アンケートした。なお、比較例は実施例より製造例の各種のシロバナチョウセンアサガオの抽出物を水にかえたものである。（比較例１，２）なお、１２名を２班にわけ、下記の表５の試料を使って実験した。
【００２５】
【表５】

【００２６】
判定基準は以下のようでこの評点の合計値をまとめたのが以下の表６である。
実施例の方が非常によい ３
実施例の方がかなりよい ２
実施例の方がややよい １
差がない ０
比較例の方がややよい −１
比較例の方がかなりよい −２
比較例の方が非常によい −３
【００２７】
【表６】

【００２８】
【発明の効果】
本発明の化粧料は、美白作用に優れ、更に活性酸素抑制作用、抗酸化作用にも優れているので、肌荒れを防止する効果が大きい。
古くより、内服薬として用いられて来たので、人体に対する安全性の面で保証されている。[0001]
[Industrial applications]
The present invention relates to a cosmetic having a high whitening effect and effective for rough skin.
[0002]
[Prior art]
Scientific name is Datsura stramonium L., the scientific name is Datura stramonium L. It is a plant of the Solanaceae family. .
Conventionally, it has been used for bronchial asthma, chronic asthmatic bronchitis, stomach pain, rheumatic pain and the like.
[0003]
On the other hand, various substances having a whitening effect that can be used as a raw material for cosmetics are known, but synthetic products have no guarantee of safety when applied to human skin for a long period of time, and are not used. It is being restricted.
On the other hand, many natural products have a weak whitening effect.
However, from the viewpoint of safety for human skin, it is a natural product, which is guaranteed for safety by many people drinking or eating it as a medicine, and has a strong whitening effect. A substance having both effects has been desired.
[0004]
[Problems to be solved by the invention]
An object of the present invention is to provide a cosmetic composition which is applied to the skin, guarantees safety, has a large whitening effect, and further contains an effective ingredient for rough skin.
[0005]
[Means for Solving the Problems]
The present inventors, in order to solve the above-mentioned problems, have been screened and examined for plants that have already been drunk as medicines or edible for many years, and that have been confirmed to be safe for the human body. I considered something.
[0006]
As a result, it has been found that white narcissus morning glory is very effective as a cosmetic raw material or as a quasi-drug.
As the confirmed effects, a whitening effect, an active oxygen suppressing effect, and an antioxidant effect were confirmed.
That is, the present invention is a cosmetic that contains an extract extracted from the seeds of Asparagus japonicum with water or a hydrophilic organic solvent, or a mixed solvent thereof.
[0007]
As a method of utilizing Aspergillus niger, extraction is performed with water or a hydrophilic organic solvent such as ethanol, methanol, or acetone. However, it is a matter of course that extraction with water, ethanol or a mixed solvent thereof is preferable because it is the extraction of cosmetic raw materials.
In some cases, a polyhydric alcohol such as glycerin, 1,3-butylene glycol, propylene glycol, or a mixture of polyhydric alcohol and water can also be used for extraction.
It is also effective to freeze-dry and use it as a powder depending on the method of use.
[0008]
This material is added with other cosmetic ingredients such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3 butylene glycol, and various chemicals. Various dosage forms of cosmetics can be prepared. For example, a use form may be considered depending on the purpose, such as a lotion, a cream, a milky lotion, and a pack.
[0009]
As described above, the effect of the extract of the present invention is firstly a whitening effect.
The second is an active oxygen suppressing action. There is oxygen in the air, without which organisms (except anaerobic ones) cannot exist. However, oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes. Active oxygen oxidizes fatty acids to form peroxides. Phospholipids in biological membranes of living organisms also oxidize and cause damage.
In addition, it is said that the generated peroxide and active oxygen damage DNA and accelerate aging. This active oxygen affects the mechanism of producing melanin from tyrosine and is also involved in skin darkening. Suppressing this active oxygen is important for the skin, in other words, an important factor required for cosmetics.
The Aspergillus vulgaris according to the present invention also has this active oxygen suppressing action and antioxidant property.
[0010]
【Example】
An embodiment which is an actual use method will be described below, but the present invention is not limited to the embodiment.
The production example of the extract of Aspergillus niger used in the present invention is shown below.
[0011]
(Production Example 1)
300 g of ethanol (300 g) was added to 10 g of the seeds of Datura stramonium (dried product), and the mixture was allowed to stand for 5 days with occasional stirring. After evaporating this, it was freeze-dried after filtration.
[0012]
(Production Example 2)
300 g of a 50% aqueous ethanol solution was added to 10 g of the seeds of a dried scutellaria, and the mixture was allowed to stand for 5 days with occasional stirring. After evaporating this, it was freeze-dried after filtration.
[0013]
(Production Example 3)
300 ml of purified water is added to 10 g of the seeds of Datura stramonium (dry product) and heated for 3 hours. This was allowed to cool, filtered, and lyophilized.
[0014]

[0015]

A and B were each weighed and heated to 70 ° C., A was gradually added to B with stirring, and then cooled to 30 ° C. with slow stirring.
[0016]
Example 3 was prepared by changing the extract of Production Example 1 of Example 1 to the extract of Production Example 3.
[0017]
(Tyrosinase activity inhibition test)
(Test method)
0.9 ml of McClbaln buffer solution, 1.0 ml of 1.66 mM tyrosine solution, 0.1 wt / v% aqueous solution of the above-mentioned production example (lyophilized product) (ethanol was added if it was difficult to dissolve) and dissolved. Thereafter, purified water was added thereto, and the mixture was evaporated and ethanol was removed. Then, 1.0 ml of the resulting mixture was adjusted to 0.1 wt / v%) was taken in a screw vial, and heated in a 37 ° C constant temperature water bath for 5 minutes or more.
0.1 ml of a tyrosinase solution (manufactured by Sigma, derived from mushrooms, 914 units / ml) was added, the temperature was kept in a constant temperature water bath at 37 ° C., and the absorbance was measured at 475 nm after 10 minutes.
As a control, pure water was added instead of the sample solution, and the measurement was performed in the same manner.
In this test, the final concentration of the sample is 0.033%.

Table 1 shows the results.
[0018]
[Table 1]

[0019]
(Active oxygen suppression test)
There are various methods for measuring the effect of suppressing active oxygen, but the following method was used this time.

2.4 ml of the mixture of the above reagents and 0.3 ml of the sample are added, and xanthine oxynase is added to the potassium phosphate buffer so that the absorbance increases by about 0.02 per minute when the experiment is carried out using water as the sample in advance. 0.1 ml of the solution (adjusted with the solution) and immediately measure the absorbance (560 nm). (Measurements are divided into two and linearity is confirmed)
Calculation formula Inhibition rate = ((AB) / A) × 100
A: Change in absorbance per minute when the sample was water B: Change in absorbance per minute of the sample Several steps were performed to find the 50% active oxygen production inhibitory concentration. A sample was prepared by dissolving the above-mentioned production example (freeze-dried product) in an aqueous solution having an appropriate concentration (if it is difficult to dissolve, adding ethanol to the solution, adding purified water to the solution, evaporating the solution, removing the ethanol, and then removing an appropriate concentration% It was prepared so that it might become.
Table 2 shows the results of the 50% active oxygen production inhibition concentration for Production Example 2.
[0020]
[Table 2]

[0021]
(Antioxidant test)
The following test solutions were prepared in 50 ml test tubes with screw caps.
Sample 5mg
10% 2% linoleic acid ethanol solution
0.1 M, pH 7.0 phosphate buffer 10 ml
5 ml of purified water
This is left in a constant temperature bath at 50 ° C., protected from light.
The following measurements were taken before putting this in a thermostat and at intervals of several days.
0.125 ml of test solution, 12.125 ml of 75% ethanol and 0.125 ml of 30% ammonium thiocyanate were added, stirred and left for 3 minutes, and 0.12 ml of 0.02N ferrous chloride 3.5% HCl aqueous solution was added. After stirring and leaving for 3 minutes, the absorbance was measured at a wavelength of 500 nm. The cell length was 10 mm, and the control cell was one in which the test solution was replaced with water.
The results are shown in Tables 3 and 4.
[0022]
[Table 3]

[0023]
[Table 4]

[0024]
(Use test)
The face of each of six women was divided into left and right, one of which was used as an example and the other was used as a comparative example at least once a day. In the comparative examples, various extracts of Aspergillus vulgaris from the examples were replaced with water. (Comparative Examples 1 and 2) Twelve people were divided into two groups, and experiments were performed using the samples shown in Table 5 below.
[0025]
[Table 5]

[0026]
The criteria are as follows, and the total of the scores is summarized in Table 6 below.
Example is much better 3
Example is considerably better 2
Example is slightly better 1
No difference 0
Comparative example is slightly better -1
Comparative example is much better -2
Comparative example is much better -3
[0027]
[Table 6]

[0028]
【The invention's effect】
The cosmetic of the present invention is excellent in whitening effect, active oxygen suppressing effect and antioxidant effect, so that the effect of preventing rough skin is great.
Since ancient times, it has been used as an oral medicine, so it is guaranteed in terms of safety for the human body.

Claims (1)

A cosmetic comprising an extract obtained by extracting water or a hydrophilic organic solvent, or a mixed solvent thereof, from seeds of Asparagus japonicum.