JP3176983B2 - Whitening cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic which has a high whitening effect, inhibits the activity of hyaluronidase, and is effective for rough skin.

[0002]

2. Description of the Related Art Tsukuniko uses the scientific name of the dicotyledonous plant net, the leaf-flowering sub-net, the chinensis, and the genus Siccunidae as Quisqualis indica L. The dried fruit of the plant called. Kunshi is an evergreen vine that lives in warm regions of China. It is used as an anthelmintic and stomach medicine, for exterminating roundworm, abdominal pain, indigestion, diarrhea, etc.

On the other hand, various substances having a whitening effect that can be used as a raw material for cosmetics are known.
Synthetic products are not guaranteed for long-term application to human skin, and their use is being restricted. On the other hand, many natural products have a weak whitening effect. However, it is a natural product that is safe for human skin, eaten by humans for many years, is guaranteed in terms of safety, has a strong whitening effect, and has other effects on skin. Was desired.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to provide a whitening cosmetic which is safe when applied to the skin, has a large whitening effect, inhibits the activity of hyaluronidase, and further contains an effective ingredient for rough skin. It is to provide.

[0005]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have screened and examined plants which have been used in medicines and the like for many years and have been confirmed to be safe for the human body. We considered something worthwhile. As a result, it has been found that Kimiko is very effective as a cosmetic raw material or as a quasi-drug. As a result, whitening action, inhibition of hyaluronidase activity, suppression of active oxygen, and antioxidant properties were confirmed.

[0006] That is, the present invention is a whitening cosmetic containing a solvent extract of Kimiko.

[0007] As a method of using the spores, extraction is carried out with water or a hydrophilic organic solvent such as ethanol, methanol or acetone. However, it is a matter of course that extraction with water, ethanol, or a mixed solvent thereof is preferable because the cosmetic raw material is extracted. Also, in some cases,
Polyhydric alcohols such as glycerin, 1,3-butylene glycol and propylene glycol, or a mixture of polyhydric alcohol and water can also be used for the extraction. It is also effective to freeze-dry and use as a powder depending on the method of use.

This substance is used as a raw material for other cosmetics such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3-butylene glycol and various chemicals. It can be added to prepare various forms of cosmetics. For example, a use form may be considered depending on the purpose, such as a lotion, a cream, an emulsion, a pack, and the like.

The effect of the extract of the present invention is, as described above, firstly a skin whitening effect. Second is the activity of suppressing the activity of hyaluronidase. Hyaluronidase is an enzyme that is widely distributed in living organisms and also present in the skin. As its name implies, it degrades hyaluronic acid. Hyaluronic acid is β-DN
-Acetyl glucosamine and β-D-glucuronic acid are alternately linked linear high-molecular polysaccharides and are a type of glycosaminoglucan widely present in connective tissues of mammals together with chondroitin sulfate. The function of hyaluronic acid in connective tissue is to hold water in the intercellular space, form a jelly-like matrix in the tissue to hold the cells, maintain the lubricity and flexibility of the skin, Disorder)
And prevent bacterial infection. It is said that the hyaluronic acid in the skin decreases with age, resulting in aging such as fine wrinkles and dryness.
Therefore, suppressing the activity of hyaluronidase, which degrades this, can improve the stability of hyaluronic acid used in the preparation, the stability of hyaluronic acid in the preparation after application to the skin, and the hyaluronic acid existing in the skin. It is thought to contribute.

Third, there is an active oxygen suppressing action. There is oxygen in the air, without which organisms (except anaerobic ones) cannot exist. However, oxygen becomes active oxygen under the influence of ultraviolet rays and enzymes. Active oxygen oxidizes fatty acids to form peroxides. Phospholipids in biological membranes of living organisms also oxidize and cause damage. In addition, it is said that the generated peroxide and active oxygen damage DNA and promote aging. This active oxygen affects the mechanism of producing melanin from tyrosine and is also involved in skin darkening. Suppressing this active oxygen is important for the skin, in other words, an important factor required for cosmetics. The essence of the present invention also has this active oxygen suppressing action and antioxidant property.

[0011]

EXAMPLES An example of an actual use method will be described below, but the present invention is not limited to this example. The following is an example of the production of the extract of Kimiko used in the present invention.

(Manufacturing Example 1) 300 ml of ethanol was added to 10 g of Kimiko (dried product) and left for 5 days with occasional stirring. This was lyophilized after filtration. (Production Example 2) 300 g of a 50% aqueous ethanol solution was added to 10 g of Kimiko (dried product) and left for 5 days with occasional stirring. This was lyophilized after filtration. (Production Example 3) Purified water 300 g in 10 g of Kimiko (dried product)
Add ml and heat for 3 hours. This was allowed to cool, filtered, and lyophilized.

(Example 1) Lotion (% by weight) Olive oil 0.5 Ethanol extract of Mikomiko of Production Example 1 0.5 Polyoxyethylene (20E.O.) Sorbitan monostearate 2.0 Polyoxyethylene ( 60E.O.) hydrogenated castor oil 2.0 ethanol 10.0 1.0% aqueous sodium hyaluronate solution 5.0 purified water 80.0

Example 2 Cream (% by weight) A Squalane 20.0 Olive oil 2.0 Mink oil 1.0 Jojoba oil 5.0 Beeswax 5.0 Cetostearyl alcohol 2.0 Glycerin monostearate 1.0 Sorbitan Monostearate 2.0 50% Ethanol extract of Mikomushi of Production Example 2 1.0 B Purified water 47.9 Polyoxyethylene (20E.O.) Sorbitan monostearate 2.0 Polyoxyethylene (60E.O. .) Hardened castor oil 1.0 Glycerin 5.0 1.0% aqueous sodium hyaluronate solution 5.0 Methyl p-hydroxybenzoate 0.1 A and B were each weighed, heated to 70 ° C, and A was stirred in B. Then slowly add, then slowly stir for 30 minutes.
Cooled to ° C.

Example 3 Example 3 is a cream prepared by changing the extract of Production Example 2 of Example 2 to the extract of Production Example 3.

(Inhibition of Tyrosinase Activity) (Test Method) Mcllvaln Buffer 0.9
ml, 1.66 mM Tyrosine solution 1.0 ml, 0.1 wt / v% aqueous solution of the above preparation example (lyophilized product) (If it is difficult to dissolve, add ethanol and then dissolve, then add purified water, and evaporate. After removing ethanol,
1.0 ml (prepared to be 0.1 wt / v%) was placed in a screw vial and heated in a 37 ° C constant temperature water bath for 5 minutes or more. 0.1 ml of a tyrosinase solution (manufactured by Sigma, from mushrooms, 914 units / ml) was added, the mixture was kept warm in a constant temperature water bath at 37 ° C., and the absorbance was measured at 475 nm after 10 minutes. As a control, pure water was added instead of the sample solution, and the measurement was performed in the same manner. In this test, the final concentration of the sample was 0.033.
%. (Calculation formula) Tyrosinase activity inhibition rate (%) = {B- (AP)} /
B × 100 where A: Absorbance of sample specimen B: Absorbance of control P: Absorbance due to coloring of sample specimen (3 times dilution)

[0017]

[Table 1] The 50% ethanol extract of Production Example 2 had no tyrosinase activity inhibitory effect.

(Test for inhibiting hyaluronidase activity) (Test method) 0.4% sodium hyaluronate 0.1M
(PH 6.0) Weigh 6 g of phosphate buffer solution,
After standing in a constant temperature water bath at 5 ° C. for 5 minutes, a 0.1 wt / v% aqueous solution of the above production example (freeze-dried product) (if it is difficult to dissolve, add ethanol and dissolve, then add purified water, evaporate, and evaporate the ethanol. After removal, 1.0 ml of the mixture was prepared so as to have a concentration of 0.1 wt / v%), and the mixture was stirred. 0.01% hyaluronidase (manufactured by Sigma Corp., bovine testes, type IS)
1 ml of a 0.1 M (pH 6.0) phosphate buffer solution was added, and the mixture was immediately stirred, and 6 ml of the solution was placed in an Ostwald viscometer placed in a thermostat bath at 37 ° C. After 1 minute, 5 minutes, 10 minutes, 20 minutes, and 40 minutes, the viscosity was measured. As a control, pure water was added instead of the sample solution, and the measurement was performed in the same manner.
In this test, the final concentration of the sample is 0.0125%. 1
The results are shown in Tables 2 and 3 as indices, with the viscosity after one minute being 100.

[0019]

[Table 2]

[0020]

[Table 3]

(Active oxygen suppression test effect) There are various methods for measuring the effect of suppressing active oxygen. pH 7.8, 50 mM potassium phosphate buffer (containing 1.3 mM DETAPAC) 133 ml 40 unit / ml catalase above potassium phosphate buffer 5 ml 2 mM nitro blue tetrazolium above potassium phosphate buffer 5 ml 1.8 mM xanthine above Potassium phosphate buffer 17 ml 160 ml 2.4 ml of the above mixture of reagents and 0.3 ml of the sample are added, and xanthine oxynase is used. 0.3 ml of the above solution (adjusted with the above potassium phosphate buffer), and immediately measure the absorbance (560 nm).
(Measurement is divided into two to confirm linearity.) Formula: Inhibition rate = ((AB) / A) × 100 A: Change in absorbance per minute when the sample is water B: Sample Changes in absorbance per minute Several concentration steps were performed to find a 50% active oxygen production inhibition concentration. The method of preparing the sample is as follows. The above-mentioned production example (lyophilized product) is dissolved in an aqueous solution of an appropriate concentration (if it is difficult to dissolve, ethanol is added thereto, and then purified water is added thereto, followed by evaporation.
After removing the ethanol, it was adjusted to an appropriate concentration%). 50% of Production Examples 1, 2, and 3
Table 4 shows the active oxygen production inhibitory concentrations.

[0022]

[Table 4] The ethanol extract of Production Example 1 had no active oxygen suppression effect.

(Antioxidant test) The following test solutions were prepared in 50 ml test tubes with screw caps. Specimen 5 mg 2% linoleic acid ethanol solution 10 ml 0.1 M, pH 7.0 phosphate buffer 10 ml purified water 5 ml Before putting it in a thermostat, the following measurements were made after 3, 6 and 8 days. Test solution 0.125 ml, 75% ethanol 12.1
25ml, 30% ammonium thiocyanate 0.125ml
After stirring for 3 minutes, 0.125 ml of a 0.02N ferrous chloride 3.5% HCl aqueous solution was added, and the mixture was stirred and left for 3 minutes. Then, the absorbance was measured at a wavelength of 500 nm. Cell length 10
mm, control cell is water with test liquid replaced. As comparative examples, vitamin E (Liken E oil 700) and dibutylhydroxytoluene (BHT) were measured.

[0024]

[Table 5] * Riken Vitamin Co., Ltd.

(Usage test) The face of each of five women was divided into left and right, one was used as an example, and the other was used as a comparative example every day.
A questionnaire was given three months later after having used it at least once.
In the comparative examples, the extracts of various kinds of mushrooms of the production examples were replaced with water. (Comparative Examples 1 and 2)
Zero people were divided into two groups, and experiments were performed using the following samples. Table 6 below summarizes the results of the questionnaire with the following criteria. Example is much better 3 Example is much better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better -2 Comparative example Is much better -3

[0026]

[Table 6]

[0027]

According to the present invention, the skin whitening effect is strong, the activity of hyaluronidase is suppressed, the lubricity and flexibility of the skin are maintained, the generation of active oxygen is inhibited, and the antioxidant effect is obtained. Prevents aging and suppresses darkening of skin. Therefore, while preventing skin roughness, maintaining the gloss of the skin, the beam,
A whitening cosmetic having high safety and excellent whitening action is provided.

Claims (1)

(57) [Claims] 1. A whitening cosmetic containing a solvent extract of Kimiko.