JP6386773B2 - Hair restorer - Google Patents

Description

  The present invention relates to a hair growth promoter and a hair growth agent.

  In many cases of alopecia such as male pattern alopecia and alopecia areata, the details of the onset mechanism are still unclear. In the past, these alopecia treatments have empirically used drugs such as blood circulation promoters, immunosuppressants, metabolic promoters, vitamins, anti-androgens, and many other plant materials. There are reports (for example, Patent Documents 1 to 3).

  However, these drugs often have different effects depending on the symptoms and constitution, and the effects are not yet satisfactory. In addition, when used in a large amount, there are cases where an uncomfortable irritation sensation is given to an adaptation site, or dermatitis occurs due to continuous use.

  On the other hand, Vicia faba, a plant belonging to the genus Broad bean, has been edible for many years around the world and contains a lot of protein, vitamin B1, vitamin B2, vitamin C, potassium, iron, copper, etc. It is known to be effective for symptom, fatigue, fatigue, summer heat, and arteriosclerosis. The plant belonging to the genus Sequimidae, Combretum indicum, has an anthelmintic action and is used as a folk medicine for abdominal pain, indigestion, and diarrhea. It has been reported (Patent Document 4). Alpinia galanga, a plant belonging to the genus Ginger, is used as a folk medicine for gastric pain, indigestion, vomiting and diarrhea, and has been reported to have carcinogenic and anti-inflammatory effects ( Non-Patent Documents 1 and 2).

  However, it has not been known so far that broad bean, shikunshi and nankyo have a hair growth promoting action.

JP-A-5-9105 JP 2003-12472 A JP 2003-238365 A JP-A-5-306215

Murakami et al., J. Nutr. 135, 2987S-2992S, 2005 Nakamura et al., Cancer Res. 58.4832-4839, 1998

  The present invention relates to promoting hair growth and providing a material effective for hair growth.

  The inventors of the present invention have found that the extracts of broad beans, shikunshi and nankyo have a hair growth promoting action, and have found that these can be used as hair growth promoting agents and hair restoration agents.

That is, the present invention relates to the following 1) to 2).
1) A hair growth promoter comprising, as an active ingredient, a plant selected from broad bean, wingworm and peanut or an extract thereof.
2) A hair-growth agent comprising as an active ingredient a plant selected from broad bean, wingworm and peanut or an extract thereof.

  By applying the hair growth promoter or hair growth agent of the present invention to the scalp or the like, excellent hair growth effects such as hair growth promotion and hair restoration are exhibited at the application site.

In this specification, "broad bean" refers to the broad bean of the leguminous family broad bean (Vicia faba). "" Refers to Nanpina (Alpinia galanga), a plant belonging to the genus Gingeraceae, and is different from the so-called ginger (Zingiber officinale of the Ginger family).
The plant of the present invention can be used for whole grass, leaves, stems, buds, flowers, buds, roots, rhizomes, seeds, fruits, or the like, or a combination thereof. It is preferable to use seeds for and for roots to use rhizomes. Such a plant can be used as it is or as a squeezed juice obtained by squeezing it, a dried product obtained by drying the plant itself or a pulverized product thereof, or an extract extracted therefrom. Is preferred.
Moreover, in this invention, you may use said plant or its extract in mixture of 2 or more types.

  Examples of such plant extracts include various solvent extracts obtained by extraction by a known extraction method, diluted solutions thereof, concentrated solutions or dried powder thereof. Examples of known extraction methods include immersion, decoction, leaching, solid-liquid extraction, reflux extraction, supercritical extraction, ultrasonic extraction, and microwave extraction. For example, the immersion may include immersion and leaching for 1 hour to 4 weeks at 0 ° C. to the solvent boiling point (preferably 15 to 40 ° C.), and the solid-liquid extraction may be performed at 0 ° C. to the solvent boiling point (preferably 15 to 40). And stirring or shaking at 30 to 1000 rpm for 30 minutes to 2 weeks. In order to prevent oxidation of the extract, a means for extracting under a so-called non-oxidizing atmosphere while removing dissolved oxygen by passing an inert gas such as boiling degassing or nitrogen gas may be used in combination. Moreover, in the case of reflux extraction, it can be performed using an extraction tool such as a Soxhlet extractor.

  As the solvent for extraction, either a polar solvent or a nonpolar solvent can be used. Specific examples of the solvent include water; alcohols such as methanol, ethanol, propanol and butanol; polyhydric alcohols such as propylene glycol and butylene glycol; ketones such as acetone and methyl ethyl ketone; methyl acetate and ethyl acetate Esters; linear and cyclic ethers such as diethyl ether and tetrahydrofuran; polyethers such as polyethylene glycol; hydrocarbons such as squalane, hexane, cyclohexane and petroleum ether; aromatic hydrocarbons such as toluene; dichloromethane and chloroform And halogenated hydrocarbons such as dichloroethane; and supercritical carbon dioxide; pyridines; organic solvents such as oils and fats, other oils such as wax; and mixtures thereof. Preferable examples include water, alcohols, and water-alcohol mixed solvents, and ethanol is preferable as the alcohols. Of these, water and an aqueous ethanol solution are preferred. The ethanol aqueous solution has an ethanol concentration of preferably 10% (v / v) or more, more preferably 20% (v / v) or more, and preferably 95% (v / v) or less, more preferably 90%. % (V / v) or less, preferably 10 to 95% (v / v), more preferably 20 to 95% (v / v).

  The plant extract of the present invention is extracted from 4 parts by weight to 100 degrees C. for 0.5 hours to 30 days using, for example, an extraction solvent of 1 part by weight to 50 parts by weight with respect to 1 part by weight of the plant body. This can be done. More specifically, when an aqueous ethanol solution is used as the extraction solvent, 5 to 30 parts by mass and 1 hour to 10 days at room temperature are preferable with respect to 1 part by mass of the plant. Further, these operations may be repeated.

  In the present invention, the above extract can be used as it is, but it can also be used after being diluted, concentrated or lyophilized and then prepared into a powder or paste. Also, freeze-dry and dilute with a solvent usually used for extraction, such as water, ethanol, propylene glycol, butylene glycol, water / ethanol mixture, water / propylene glycol mixture, water / butylene glycol mixture, etc. It can also be used. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

  The plant extract of the present invention may be a crude product as long as it conforms to, for example, a cosmetically or pharmaceutical acceptable standard and exhibits the effects of the present invention. In addition, if necessary, treatments such as removal of inert impurities, deodorization, and decolorization may be performed by known techniques such as liquid-liquid distribution, solid-liquid distribution, filtration membrane, activated carbon, adsorption resin, ion exchange resin, and the like. Further, the purity may be increased by appropriately combining known separation and purification methods. Examples of the purification means include organic solvent precipitation, centrifugation, ultrafiltration membrane, high performance liquid chromatograph, column chromatograph and the like.

  As shown in Examples described later, the plant extracts of broad beans, shikunshi and nankyo have an effect of significantly promoting the growth of organ culture hair. Therefore, a plant selected from broad bean, winged beetle and peanut, or an extract thereof (hereinafter referred to as “the plant of the present invention or an extract thereof”) can be used to promote hair growth and can be a hair growth promoter. Moreover, since the hair growth effect or the effect of preventing or suppressing hair loss can be exerted through the hair growth promoting action, the plant of the present invention or an extract thereof can be used for hair loss prevention or hair growth, and hair loss prevention. It can be an agent or hair restorer. Moreover, the plant of this invention or its extract can be used in order to manufacture a hair growth promoter, a hair loss prevention agent, or a hair growth agent. Here, the use using the plant of the present invention or an extract thereof may be a use for human or non-human animals, and may be a therapeutic use or a non-therapeutic use. Here, “non-therapeutic” means a concept that does not include medical practice, that is, a concept that does not include a method for operating, treating, or diagnosing a person, more specifically, a doctor or a person who has received instructions from a doctor It is a concept that does not include a method for performing surgery, treatment, or diagnosis on the subject.

Here, “hair growth promotion” means an action of promoting the elongation of hair or hair follicles or an action of expanding hair diameter.
“Hair growth” means, for example, at least one of hair growth, promotion of hair growth and prevention of hair loss, and is a concept including hair growth, promotion of hair growth (elongation), or hair growth.

  The plant of the present invention or an extract thereof can be a cosmetic, a pharmaceutical product or a quasi-drug for promoting hair growth, preventing hair loss or growing hair, or a cosmetic or pharmaceutical product for promoting hair growth, preventing hair loss or hair growth. It can be a raw material or a raw material for producing a quasi drug.

  The form of various pharmaceutical compositions such as cosmetics, pharmaceuticals and quasi-drugs containing the plant of the present invention or an extract thereof may be either oral or parenteral administration, but is parenteral administration. Is preferable, and a skin external preparation is more preferable. Specifically, it can be used in various forms such as ointments, emulsified cosmetics, creams, emulsions, lotions, gels, aerosols, among others, hair rinses, hair conditioners, hair treatments, hair lotions, hair tonics, hair packs, Hair cream, conditioning mousse, hair mousse, hair spray, shampoo, leave-on treatment and the like are preferred.

  The above-mentioned pharmaceutical composition is prepared by a general production method, respectively, according to the plant of the present invention or an extract thereof, or a pharmaceutically acceptable carrier such as various oils, surfactants, gelling agents, preservatives, antioxidants. It can be obtained by mixing and dispersing together with a solvent, alcohol, water, chelating agent, thickener, ultraviolet absorber, emulsion stabilizer, pH adjuster, pigment, fragrance, etc., and then processing into a desired form. In addition to the plant of the present invention or an extract thereof, these pharmaceutical compositions include other plant extracts, fungicides, as appropriate, depending on the types of preparations such as cosmetics, quasi drugs, and pharmaceuticals, respectively. Medicinal components such as a humectant, an antibacterial agent, and a refreshing agent can be appropriately blended within a range that does not interfere with the effects of the present invention. In order to further enhance the hair-growth effect, other hair-growth ingredients (for example, anti-inflammatory agents, cell activators, sebum secretion inhibitors, peripheral vasodilators, amino acids, vitamins, etc.) can be appropriately blended.

  The content of the plant of the present invention or the extract thereof in the pharmaceutical composition is generally preferably 0.001% by mass or more, more preferably 0.01% by mass in terms of the dry weight of the extract. Above and preferably 99% by mass or less, more preferably 20% by mass or less, and preferably 0.001 to 99% by mass, more preferably 0.01 to 20% by mass.

The dosage of the above pharmaceuticals and quasi drugs is not particularly limited as long as the effect of the present invention can be obtained, and may vary according to the condition, weight, sex, age or other factors of the subject. 60 kg) per day per person, for example, preferably 0.01 mg or more, more preferably 0.1 mg or more, and preferably 1000 mg or less, more preferably 100 mg as the plant of the present invention or an extract thereof (in terms of dry matter) It is. Moreover, Preferably it is 0.01-1000 mg, More preferably, it is 0.1-100 mg. The preparation can be ingested / administered according to any ingestion / administration plan, but is preferably divided into once to several times a day and continuously administered for several weeks to several months.
In addition, the application target of the cosmetic, pharmaceutical product, or quasi-drug is not particularly limited as long as it is required, but a human for the purpose of promoting hair growth, preventing hair growth or preventing hair loss is preferable.

Regarding the above-described embodiment, the following aspects are further disclosed in the present invention.
<1> A hair growth promoter comprising, as an active ingredient, a plant selected from broad beans, shikunshi and nankyo, or an extract thereof.
<2> A hair loss preventive agent comprising a plant selected from broad bean, chikunshi and nankyo or an extract thereof as an active ingredient.
<3> A hair restorer comprising as an active ingredient a plant selected from broad beans, shikunshi and nankyo or an extract thereof.

<4> Use of a plant or extract thereof selected from broad beans, shikunshi and nankyo for producing a hair growth promoter.
<5> Use of a plant or extract thereof selected from broad beans, shikunshi and nankyo for producing a hair loss inhibitor.
<6> Use of a plant or extract thereof selected from broad beans, shikunshi and nankyo for producing a hair restorer.
<7> A plant or extract thereof selected from broad beans, shikunushi and nankyo for use in promoting hair growth.
<8> A plant or extract thereof selected from broad beans, shikunushi and nankyo for use in preventing hair loss.
<9> A plant selected from broad beans, shikunshi and nankyo or extract thereof for use in hair growth.
<10> A method for promoting hair growth using a plant selected from broad beans, shikunushi and nankyo or an extract thereof.
<11> A method for preventing hair loss using a plant selected from broad beans, shikunushi and nankyo or an extract thereof.
<12> A hair-growth method using a plant selected from broad beans, shikunushi and nankyo or an extract thereof.
<13> The method <10> to <12>, which is non-therapeutic.
<14> The method according to <7> to <9>, wherein the use is non-therapeutic use.
<15> In the extracts of <1> to <14>, it is preferable to extract the seeds for broad beans, the seeds for shikunshi, and the rhizomes for peanut.
<16> Solvents for extracting the extracts of <1> to <15> are preferably water, alcohols and water-alcohol mixed solvents, and ethanol is preferred as the alcohols. Of these, water and an aqueous ethanol solution are preferred.
<17> The ethanol concentration in the aqueous ethanol solution of <16> is preferably 10% (v / v) or more, more preferably 20% (v / v) or more, and preferably 95% (v / v) or less. More preferably, it is 90% (v / v) or less, preferably 10 to 95% (v / v), more preferably 20 to 95% (v / v).

Production Example 1 Production of broad bean extract To 50 g of ground bean seeds (Vicia faba), 0.5 L of 50% ethanol aqueous solution was added and immersed for 7 days at room temperature. This was filtered to obtain a broad bean extract. When this broad bean extract was concentrated, the solid content was 5.16 g. This extract was diluted with a 50% aqueous ethanol solution to give a 1% (w / v) solution.

Manufacture example 2 Manufacture of the extract of Sikkunshi 0.5L of 50% ethanol aqueous solution was added to 50 g of seed pulverized matter of Sykkunshi (Combretum indicum), and it was immersed for 7 days at room temperature. This was filtered to obtain a squeeze extract. When this Sikkunshi extract was concentrated, the solid content was 5.5 g. This extract was diluted with a 50% aqueous ethanol solution to give a 1% (w / v) solution.

Manufacture example 3 Manufacture of a Nanko extract The 50% ethanol aqueous solution 0.5L was added to 50 g of rhizomes of Alpinia galanga, and it was immersed for 7 days at room temperature. This was filtered to obtain a Nanko extract. When the Nanko extract was concentrated, the solid content was 19.9 g. This extract was diluted with a 50% aqueous ethanol solution to give a 1% (w / v) solution.

Example 1 Evaluation of hair growth promoting effect using pig isolated hair follicle (1) The buttocks skin obtained from pigs slaughtered for meat at a meat processing plant was cut into appropriate sizes, and excess adipose tissue Was excluded. After being immersed in the Hibiten solution for 20 minutes, it was washed 3 times with PBS (Ca and Mg free, Life Technologies, 1% antibacterial agent included). After washing, sterile gauze (white cross sterase) was spread on a 10 cm diameter petri dish, an appropriate amount of PBS was added to prevent the skin from drying, and pork skin was placed on the gauze. Next, using tweezers and a scalpel (FEATHER No 10) under the actual microscope, they were isolated one by one, taking care not to damage the hair follicles, and recovered in the medium. William's Medium E (SIGMA) was used as the medium. As additives, 2 mM L-glutamine (Life Technologies) and 1% (V / V) antibiotics (Life Technologies) 10 μg / ml hydrocortisone (SIGMA) and 0.01 μg / ml insulin (SIGMA) were added.
The isolated hair follicles were placed one by one in a 24-well plate containing the above medium and cultured under conditions of 37 ° C. and 5% CO ₂ . Photographs were taken at the start of culture and after 2 days of culture, and image analysis was performed.

(2) From the image analysis results, the hair follicles elongated by 0.2 mm or more in 2 days were transferred to the medium to which the test substance was added. As the test substance, each plant extract prepared in Production Examples 1 to 3 (final concentration 0.01% (w / v)) was used, and the same amount of 50% ethanol solution was used as a control (control).
After culturing with the test substance-added medium, photography and medium exchange were performed over time. Image analysis was performed from the photographs, the amount of hair follicle elongation was measured, and the ratio of the amount of elongation of each plant extract added group to the amount of elongation of the control group was determined as the hair elongation acceleration rate by each plant extract. . Furthermore, the difference in elongation between the control group and each plant extract addition group was statistically analyzed (non-paired t-test vs control).

(3) Results The results are shown in Tables 1-3. In each of the control group and each plant extract addition group, hair follicle elongation (hair growth) was observed with the lapse of culture time, but in each plant extract addition group, the hair follicle was compared with the control group. The elongation of was promoted.

Claims (2)

A hair growth promoter comprising, as an active ingredient, a broad bean ethanol aqueous extract , a plant selected from Sikkun and Nankyo , or a plant extract selected from Sikkun and Nankyo . A hair-growth agent comprising, as an active ingredient, an aqueous ethanol extract of broad beans , a plant selected from Sikkunshi and Nankyo , or an extract of a plant selected from Sikkunshi and Nanko .