JP6267957B2 - Sebum synthesis inhibitor - Google Patents

Description

  The present invention relates to a sebum synthesis inhibitor containing an extract of Hermanus as an active ingredient, and various compositions such as pharmaceuticals, quasi-drugs, cosmetics and foods and drinks containing the sebum synthesis inhibitor.

  Sebum is synthesized by sebaceous gland cells in the sebaceous glands and secreted to the skin surface. Secreted sebum prevents the evaporation of moisture from the body by covering the skin, and has important functions such as physical and chemical stimulation from the outside, and the invasion of highly pathogenic bacteria and molds. It's done. For this reason, lack of sebum secretion in the skin causes skin problems such as dry skin and acne, and various skin diseases such as sebum-deficient dermatitis and eczema (xeroderma) (Non-Patent Documents 1 and 2). ). Sebum also has a function of supplying necessary and appropriate oil to the hair, and is an effective component for maintaining the suppleness and beauty of hair.

  As described above, sebum plays an important role for the skin and hair, but it is not cosmetically favorable in the skin, such as shine, stickiness and makeup loss, and in the scalp, an increase in dandruff, seborrheic dermatitis, accompanying it Causes hair loss. In addition, it is known that excessive sebum causes various skin troubles in the skin and scalp by helping the propagation of microorganisms and pathogens.

  For the purpose of solving or preventing problems such as dermatitis, skin troubles, cosmetics, and makeup caused by excessive secretion of sebum, it has been conventionally performed to remove sebum by washing with soap or the like. . However, the effect of removing excess sebum by washing the skin and scalp with soap etc. is temporary, suppressing excessive secretion of sebum, dermatitis due to excessive secretion of sebum, skin trouble, etc. There is a problem that cannot be improved.

  So far, studies have been made on sebum secretion inhibitors in order to improve dermatitis caused by excessive secretion of sebum as described above. As a component which has sebum secretion inhibitory action, for example, a winter melon extract (patent document 1), a rose family plant extract (patent documents 2 and 3), a karin extract (patent document 4), etc. are reported. However, although these plant extracts are highly safe natural ingredients, their actions are mild and the effects are not fully satisfactory, so the development of more durable sebum synthesis and secretion inhibitors Is desired.

  Based on previous studies, it is known that sebaceous gland cells are produced by the differentiation of their progenitor cells (sebaceous gland undifferentiated cells), and sebum synthesized by sebaceous gland cells is secreted by sebaceous gland cell rupture. (Non-patent Document 3). Therefore, in order to fundamentally regulate sebum secretion, it is necessary not only to control sebum synthesis / secretion of differentiated and sebaceous gland cells, but also to appropriately control the differentiation process starting from sebaceous gland undifferentiated cells.

  Hermanus (scientific name: Rosa rugosa) belongs to the genus Rosaceae, and is a deciduous shrub that grows naturally in Japan, the Korean Peninsula, northern China, and other places. Hermanus has many varieties and is used for ornamental use as a raw material for dyes and fragrances, and the fruit is also used as an edible material such as rose hips. Hermanus is rich in vitamin C, and so far, in addition to skin beautifying and antioxidant effects, testosterone 5α-reductase inhibitory action (Patent Document 5), hair restoration action such as hair loss prevention and hair growth effect (Patent Document 6) ), Α-amylase activity inhibitory action, α-glucosidase activity inhibitory action (Patent Document 7) and the like. However, nothing has been known about the effect of suppressing the differentiation of sebaceous gland undifferentiated cells into sebaceous cells and the effect of suppressing sebum synthesis by sebaceous cells.

JP 2008-37764 A JP2012-229167A JP2012-229169 JP2013-323331A JP-A-5-17365 JP-A-7-277930 JP-A-2005-306801

Ayako Yamamoto, Cosmetic Society Journal, 1991, 1 (4), 247-249 Susumu Takayasu, Fragrance Journal, 1998, 92, 10-13 Hinde E., Exp Dermatology, 2013, 22, 631-637

  An object of the present invention is to provide a sebum synthesis inhibitor that suppresses sebum synthesis in sebaceous gland cells, radically improves the sebum excess state in the skin and scalp, and has a durable effect.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that the extract of Hermanus suppresses sebum synthesis by sebaceous gland cells as well as suppresses differentiation of sebaceous gland undifferentiated cells into sebaceous cells. As a result, the present invention has been completed.

That is, the present invention includes the following inventions.
(1) A sebum synthesis inhibitor containing an extract of Hermanus as an active ingredient.
(2) An agent for suppressing differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells, which contains an extract of Hermanus as an active ingredient.
(3) A composition for external use on skin, comprising the agent according to (1) or (2).
(4) The external composition for skin according to (3), wherein the external composition for skin is a pharmaceutical product or a quasi-drug.
(5) The external composition for skin according to (3), wherein the external composition for skin is a cosmetic.
(6) Food / beverage products containing the agent as described in (1) or (2).
(7) The food or drink according to (6), wherein the food or drink is a health food, a functional food, a food for specified health use, or a dietary supplement.

  Since the sebum synthesis inhibitor of the present invention can suppress sebum synthesis by sebaceous gland cells, it can prevent, ameliorate, or treat skin diseases such as stickiness of the skin and scalp and seborrheic eczema caused by excessive sebum. . In addition, the extract of Hermanus, which is an active ingredient of the sebum synthesis inhibitor of the present invention, also has an action of suppressing the differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells, so sebum gland cells that are sebum synthesis and secretion organs are reduced. In addition, the state of excess sebum can be fundamentally improved, and a durable effect can be obtained. Moreover, since the sebum synthesis inhibitor of the present invention uses a plant extract with a mild action as an active ingredient, it has no side effects and is highly safe. Therefore, it can be used with confidence in cosmetics, pharmaceuticals, quasi drugs, and food and drinks.

The present invention will be described in detail below.
The sebum synthesis inhibitor of the present invention contains an extract of Hermanus as an active ingredient. Examples of the hermanus used in the present invention include hermanus (scientific name: Rosa rugosa Thunb.), Jaemanus (scientific name: Rosa rugosa Thunb. Var. Plena), Maikai (scientific name: Rosa rugosa Thunb. Var. Plena Regel), and the like.

  In the present invention, the extract of Hermanus refers to an extract of the whole plant (whole plant), a part of the plant such as leaves, stems, flowers, buds, berries, seeds, roots or a mixture thereof. A leaf extract is preferred. In addition, these plants may be used as they are for extraction, or may be subjected to treatments such as drying, pulverization and shredding.

The extraction method is not particularly limited, and can be performed by stirring or column extraction using water or hot water, or a mixed solvent of water and an organic solvent. As the organic solvent, alcohols, ethers, esters, and the like can be used, but water-soluble organic solvents such as ethanol, methanol, and acetone are preferable, and one or more of these may be used.
Particularly preferred extraction solvents include water or water-ethanol mixed polar solvents. The amount of the solvent used is not particularly limited. For example, it may be 10 times or more, preferably 20 times or more of the leaves of the above-mentioned hermanus (dry weight), but it may be concentrated or isolated after extraction. For convenience of operation, it is preferably 100 times or less. Moreover, although extraction temperature and time depend on the kind of solvent to be used, for example, it is 10-100 degreeC, Preferably it is 30-90 degreeC, 30 minutes-24 hours, Preferably it can illustrate 1 to 10 hours. In addition, the extract may be used as it is in the extracted solution, but if necessary, in a range that does not affect the effect, concentration (concentration by organic solvent, vacuum concentration, membrane concentration, etc.), dilution, filtration, You may use, after performing processing, such as decoloring by activated carbon, deodorizing, ethanol precipitation. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.

  In the present invention, the “inhibition of sebum synthesis” refers to the inhibition of synthesis of lipids mainly composed of fatty acid glycerin esters in sebaceous gland cells. Inhibition of differentiation induction from sebaceous gland undifferentiated cells to sebaceous gland cells, and synthesis in sebaceous gland cells. Moreover, the secretion suppression to the skin surface of the said lipid is also included. Therefore, the sebum synthesis inhibitor of the present invention can also be used as a differentiation inhibitor from sebaceous gland undifferentiated cells to sebaceous gland cells.

  The sebum synthesis inhibitor of the present invention can also be used as an additive for cell culture and a research reagent for maintaining the undifferentiated state of sebaceous gland undifferentiated cells.

  The sebum synthesis inhibitor of the present invention preferably also contains vitamin C, vitamin C derivative, vitamin E or vitamin E derivative in addition to the extract of Hermanus. By using these vitamins in combination, a higher sebum synthesis inhibitory effect can be obtained. Examples of vitamin C derivatives include phosphates of ascorbic acid, fatty acid esters of ascorbic acid, glycosides of ascorbic acid such as ascorbic acid glucoside, and salts thereof. As salts of vitamin C or vitamin C derivatives, alkali metal salts such as sodium salt and potassium salt, alkaline earth metal salts such as calcium salt and magnesium salt, organic amine salts such as ammonium salt, triethanolamine salt and triethylamine salt Basic amino acid salts such as lysine salts and arginine salts are preferred. Specific examples of vitamin C derivatives that can be preferably used in the present invention include magnesium ascorbate phosphate (APM), sodium ascorbyl palmitate (APPS), sodium ascorbate phosphate (APS), and ascorbine phosphate. Examples include aminoprolyl acid, glucoside ascorbate, ascorbyl palmitate, ascorbyl stearate, sodium ascorbate sulfate, ascorbyl tetraisopalmitate (VC-IP), and the like. One of these vitamin C derivatives may be used, or two or more thereof may be used in combination. Examples of vitamin E derivatives include sodium α-tocopheryl phosphate (VEP), tocopherol acetate, tocopherol nicotinate, tocopherol succinate, tocopherol linoleate, tocopherol ferulate, vitamin E glucoside, and the like. One of these vitamin E derivatives may be used, or two or more thereof may be used in combination. Moreover, you may contain both a vitamin C derivative and a vitamin E derivative.

  When the sebum synthesis inhibitor of the present invention is administered in vivo, it can be administered as it is, but it is provided in combination with an external composition with appropriate additives as long as the effects of the present invention are not impaired. It is preferable. The skin external composition of the present invention includes pharmaceuticals, quasi drugs, cosmetics and the like.

  Since the sebum synthesis inhibitor of the present invention can suppress the synthesis of sebum by sebaceous gland cells, the external composition for skin containing the agent can be used for various skins that are thought to be caused by increased or excessive sebum secretion. Effective in preventing, ameliorating, and treating disability and damage. Here, skin damage or damage caused by increased or excessive sebum secretion is not limited, but seborrheic eczema, acne vulgaris, atopic dermatitis, oily skin, horn plugs, open pores , And darkening of pores, and also includes disorders and damage to the scalp and hair such as oily dandruff, stickiness of the scalp and hair, and seborrheic alopecia.

  When the sebum synthesis inhibitor of the present invention is added to pharmaceutical products, it should be mixed with pharmacologically and pharmaceutically acceptable additives and formulated into various preparations suitable for application to the affected area. Can do. Pharmacologically and pharmaceutically acceptable additives include excipients, thickeners, tonicity agents, pH regulators, stabilizers, preservatives, preservatives depending on the dosage form and application. , Dispersants, emulsifiers, gelling agents, pigments, fragrances and the like can be used. A form suitable for the pharmaceutical product of the present invention is an external preparation, and examples thereof include an ointment, a cream, a gel, a liquid, and a patch. The ointment refers to a homogeneous semi-solid external preparation, and includes an oily ointment, an emulsion ointment, and a water-soluble ointment. The gel is an external preparation in which a water-insoluble component hydrate compound is suspended in an aqueous liquid. The liquid preparation refers to a liquid external preparation and includes lotions, suspensions, emulsions, liniments and the like.

  When the sebum synthesis inhibitor of the present invention is blended into a quasi-drug or cosmetic, the dosage form is an aqueous solution system, a solubilization system, an emulsification system, a powder system, a powder dispersion system, an oil liquid system, a gel system, or an ointment. Any of a system, an aerosol system, a water-oil two-layer system, or a water-oil-powder three-layer system may be used. In addition to the sebum synthesis inhibitor, the quasi-drug and cosmetics are selected from various ingredients, additives, bases, and the like that are usually used in compositions for external use according to the type, and are appropriately blended. It can be produced according to techniques known in the art. The form may be liquid, emulsion, cream, gel, paste, spray or the like. Examples of the ingredients include oils and fats (olive oil, coconut oil, evening primrose oil, jojoba oil, castor oil, hydrogenated castor oil, etc.), waxes (lanolin, beeswax, carnauba wax, etc.), hydrocarbons (liquid paraffin, squalene, Squalane, petrolatum, etc.), fatty acids (lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid etc.), higher alcohols (myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol etc.), esters (myristin) Isopropyl acid, isopropyl palmitate, cetyl octanoate, glyceryl trioctanoate, octyldodecyl myristate, octyl stearate, stearyl stearate, etc., organic acids (citric acid, lactic acid, α-hydroxyacetic acid, pyrrolidone cal Acid, etc.), sugars (maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, etc.), proteins and protein hydrolysates, amino acids and salts thereof, vitamins, plant / animal extracts, various interfaces Examples include activators, humectants, ultraviolet absorbers, antioxidants, stabilizers, preservatives, bactericides, and fragrances.

  The types of quasi-drugs and cosmetics include, for example, lotions, emulsions, gels, cosmetics, general creams, sun creams, packs, masks, facial cleansers, cosmetic soaps, foundations, funniers, bath preparations, body lotions, Examples include body shampoos, hair shampoos, hair conditioners, scalp lotions, scalp creams, hair tonics, and hair restorers.

  The content of the sebum synthesis inhibitor in the composition for external use of the present invention is not particularly limited as long as it is an amount capable of exerting a sebum synthesis inhibitory effect in sebaceous gland cells as well as an action of inhibiting differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells. For example, 0.00001 to 10% by weight is preferable as the dry solid weight of the extract of Hermanus, and 0.0001 to 0.01% by weight is more preferable. The above amounts are merely examples, and may be appropriately set and adjusted in consideration of the type and form of the composition, the general usage amount, efficacy / effect, cost, and the like.

  Moreover, the sebum synthesis inhibitor of this invention can be mix | blended with food-drinks. In the present invention, the food / beverage product is used to mean including a health food, a functional food, a dietary supplement, or a food for specified health use. The form of the food or drink may be any form suitable for edible use, for example, solid, liquid, granular, granular, powder, capsule, cream, or paste.

  The types of food and drink include bread, noodles, confectionery, dairy products, processed fishery and livestock products, processed oils and fats, processed foods, seasonings, various beverages (soft drinks, carbonated drinks, beauty drinks, nutritional drinks, fruit drinks) , Milk beverages, etc.) and concentrated beverages and powders for adjustment of the beverages, but are not limited thereto.

  The food / beverage products of the present invention may be appropriately blended with additives usually used depending on the type. As the additive, any food hygiene-acceptable additive can be used. For example, sweeteners such as glucose, sucrose, fructose, isomerized liquid sugar, aspartame, stevia; citric acid, malic acid, Acidic agents such as tartaric acid; excipients such as dextrin and starch; binders, diluents, fragrances, colorants, buffers, thickeners, gelling agents, stabilizers, preservatives, emulsifiers, dispersants, suspensions Agents, preservatives, etc.

  The compounding amount of the extract of the genus in the food and drink of the present invention may be any amount that can exert an action of suppressing differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells and an action of suppressing sebum synthesis in sebaceous gland cells. May be set as appropriate in consideration of typical intake, form of food and drink, efficacy / effect, taste, palatability and cost.

Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to these.
[Example 1]
Hermanus extract was prepared as follows.

(Production Example 1) Preparation of hot water extract of Hermanus leaf 1 L of purified water was added to 50 g of Hermanus (dried product of leaf), extracted at 90-100 ° C for 2 hours, filtered, and the filtrate was concentrated and compressed. Then, 5 g of hot water extract of Hermanus leaves was obtained by freeze-drying.

(Production Example 2) Preparation of 50% ethanol extract of Hermanus leaves 1 L of 50% ethanol aqueous solution was added to 50 g of Hermanus (dried product of leaves) and extracted at room temperature for 1 week. After filtration, the filtrate was concentrated under reduced pressure, and freeze-dried to obtain 4.5 g of 50% ethanol extract of Hermanus leaves.

(Production Example 3) Preparation of ethanol extract of Hermanus leaves 1 L of ethanol was added to 50 g of Hermanus (dried product of leaves) and extracted at room temperature for 1 week. After filtration, the filtrate was concentrated under reduced pressure and freeze-dried to obtain 4 g of an ethanol extract of Hermanus leaves.

(Production Example 4) Preparation of hot water extract of Hermanus whole plant 1 L of purified water was added to 50 g of Hermanus (dried whole plant), extracted at 90-100 ° C. for 2 hours, filtered, and the filtrate was concentrated. And lyophilized to obtain 4.8 g of a hot water extract of whole herbaceous grass.

(Production Example 5) Preparation of 50% ethanol extract of Hermanus whole plant 1 L of 50% ethanol aqueous solution was added to 50 g of Hermanus (dried whole plant) and extracted at room temperature for one week. After filtration, the filtrate was concentrated under reduced pressure, and freeze-dried to obtain 4.3 g of 50% ethanol extract of whole herbaceous grass.

(Production Example 6) Preparation of ethanol extract of whole herbaceous grass 1 L of ethanol was added to 50 g of hermanus (dried whole plant) and extracted at room temperature for one week. After filtration, the filtrate was concentrated under reduced pressure and freeze-dried to obtain 3.8 g of an ethanol extract of whole grass.

(Manufacture example 7) Preparation of hot water extract of herbaceous fruit 1 L of purified water was added to 50 g of herbaceous fruit (dried product), extracted at 90-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and frozen. It dried to obtain 4.5 g of hot water extract of hamanas fruit.

(Manufacture example 8) Preparation of 50% ethanol extract of red sesame seeds 1 L of 50% ethanol aqueous solution was added to 50 g of red sesame seeds (fruit dried product) and extracted at room temperature for 1 week. After filtration, the filtrate was concentrated under reduced pressure, and freeze-dried to obtain 4.2 g of a 50% ethanol extract of the genus botanus.

(Manufacture example 9) Preparation of the ethanol extract of a red sesame seed 1L of ethanol was added to 50 g of a red sesame (fruit dried product), followed by extraction at room temperature for 1 week. After filtration, the filtrate was concentrated under reduced pressure, and freeze-dried to obtain 3.5 g of an extract of genus realum.

[Example 2]
An experiment for evaluating the effect of the extract of Hermanus was performed as follows.
(Test Example 1) Evaluation of differentiation inhibitory effect from sebaceous gland undifferentiated cells to sebaceous gland cells The effect of the extract of Hermanus manufactured in Example 1 (Production Examples 1 to 9) on the differentiation efficiency from sebaceous gland undifferentiated cells to sebaceous gland cells The effect was evaluated using the expression of Pparg (Peroxisome Proliferator-Activated Receptor γ), Plin1 (Perilipin1), and Fabp4 (Fatty Acid Binding Protein 4) as specific markers of sebaceous gland cells. The specific method is described below.

5 x 10 ⁴ hamster sebaceous gland undifferentiated cells (manufactured by KURABO) cultured in hamster sebaceous gland cell growth medium (manufactured by KURABO) are seeded in 24 well dishes at a time, and then the medium is sebaceous gland cell differentiation inducing medium (manufactured by KURABO). ) To induce differentiation. At that time, the extract of Hermanus produced in Example 1 (Production Examples 1 to 9) was added so that the final concentration was 0.001%, and the culture was continued for 6 days.

  Cells on the 6th day after differentiation induction were washed twice with PBS (−), and RNA was extracted from the cells with RNA iso Plus (manufactured by Takara). Using 2-STEP real-time PCR kit (Applied Biosystems), RNA is reverse transcribed to cDNA, then ABI7300 (Applied Biosystems) is used for real-time PCR with the above primer genes (Pparg, Plin1, Fabp4) amplification primer set (95 ° C .: 15 seconds, 60 ° C .: 30 seconds, 40 cycles) was carried out to confirm gene expression of each gene. Other operations were carried out in accordance with established methods.

(Primer set for Pparg gene)
Forward primer: 5'-ATGTCTCACAATGCCATCAGGTT-3 '(SEQ ID NO: 1)
Reverse primer: 5'-CCGCCAACAGCTTCTCCTT-3 '(SEQ ID NO: 2)
(Primer set for Plin1 gene)
Forward primer: 5'-TCTGAGCTGAAAGGCACCATCT-3 '(SEQ ID NO: 3)
Reverse primer: 5'-GATGGGCACACTGATGCTGTT-3 '(SEQ ID NO: 4)
(Fabp4 gene primer set)
Forward primer: 5'-TGGCCAAACCCATCATGAT-3 '(SEQ ID NO: 5)
Reverse primer: 5'-TGTGCTCTCTGTTCGGATGGT-3 '(SEQ ID NO: 6)
(Internal standard glyceraldehyde 3-phosphate dehydrogenase (Gapdh) gene primer set)
Forward primer: 5'-TCACATGTCGCCTGGAGAAAG-3 '(SEQ ID NO: 7)
Reverse primer: 5'-GCCTTCGGATGCCTGCTT-3 '(SEQ ID NO: 8)

  For the differentiation induction efficiency of each cell into sebaceous gland cells, the expression level of each gene mRNA in the cells induced to differentiate without adding the extract of Hermanus was calculated as a ratio to the expression level of Gapdh mRNA as an internal standard. The relative expression level of each gene (the expression level of each gene / the expression level of the Gapdh gene) was set to 1.0. On the other hand, the value of the relative expression level of each gene in the cells cultured by adding the extract of Hermanus was calculated. evaluated. The results are shown in Table 1.

  As shown in Table 1, all of the extracts of Hermanus (Production Examples 1 to 9) showed a remarkable effect of suppressing differentiation induction from sebaceous gland undifferentiated cells to sebaceous gland cells.

(Test Example 2) Evaluation of sebum synthesis inhibitory effect 5 × 10 ⁴ hamster sebaceous gland undifferentiated cells cultured using a hamster sebaceous gland cell growth medium are seeded in 24 well dishes, and the medium is replaced with a sebaceous gland cell differentiation-inducing medium. Thus, differentiation induction was performed. At that time, the extract of Hermanus produced in Example 1 (Production Examples 1 to 9) was added so that the final concentration was 0.001%, and the culture was continued for 12 days. After 12 days of culture, the cells were collected, washed twice with PBS (−), and the lipid synthesized in the sebaceous gland cells was measured according to a predetermined method using a lipid synthesis measurement kit (manufactured by KURABO).

  The sebum synthesis inhibitory effect is controlled by the amount of lipid synthesis in cells cultured without adding the sample as a control (1.0), and the ratio of the amount of lipid synthesis in cells cultured with the sample added to the amount of lipid synthesis in the control ( Control ratio) was calculated. The results are shown in Table 2.

  As shown in Table 2, all of the extract of Hermanus (Production Examples 1 to 9) showed a remarkable effect of suppressing sebum synthesis in sebaceous gland cells.

[Example 3] Formulation example of product The formulation example of the product which mix | blended the extract of Hermanus manufactured in manufacture examples 1-9 is shown below.
(Formulation Example 1) Lotion Formulation Blending amount (% by weight)
1. Hermanus extract 0.01
2. 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Magnesium phosphate ascorbate (APM) 0.1
8). Ethanol 5.0
9. Methyl paraoxybenzoate 0.1
10. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
11. Fragrance 0.1
12 Purified water remaining

  Components 1 to 7 and 12 and components 8 to 11 are uniformly dissolved, and then mixed and filtered to prepare a lotion.

(Formulation example 2) Cream Formulation Blending amount (% by weight)
1. Hermanus extract 0.1
2. Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.2
12 Ethyl paraoxybenzoate 0.05
13.1,3-Butylene glycol 8.5
14 α-Tocopheryl sodium phosphate (VEP) 0.5
15. Purified water remaining

  Ingredients 2-9 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 11 to 15 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. Next, the aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

(Prescription Example 3) Emulsion
Formulation amount (% by weight)
1. Hermanus extract 0.01
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. Magnesium phosphate ascorbate (APM) 0.1
14 Purified water remaining

  Ingredients 2 to 8 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 10-14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring.

(Formulation Example 4) Gel formulation Formulation amount (% by weight)
1. Hermanus extract 0.1
2. Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil 0.1
5. Perfume proper amount 6.1,3-butylene glycol 5.0
7). Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. Purified water remaining

  Ingredients 2 to 5 and ingredients 1 and 6 to 11 are uniformly dissolved, and both are mixed to obtain a product.

(Formulation Example 5) Acne for acne treatment Formulation Formulation amount (% by weight)
1. Hermanus extract 2.0
2. Polyoxyethylene cetyl ether (30E.O.) 2.0
3. Glycerol monostearate 10.0
4). Liquid paraffin 5.0
5. Cetanol 6.0
6). Methyl paraoxybenzoate 0.1
7). Propylene glycol 10.0
8). Purified water remaining

  Ingredients 2 to 5 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 6 to 8 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the product is cooled to 30 ° C. with stirring to obtain a product.

(Formulation example 6) Pack
Formulation amount (% by weight)
1. Hermanus extract 0.1
2. Polyvinyl alcohol 12.0
3. Ethanol 5.0
4.1,3-Butylene glycol 8.0
5. Methyl paraoxybenzoate 0.2
6). Paraoxyethylene hydrogenated castor oil (20 EO) 0.5
7). Citric acid 0.1
8). Sodium citrate 0.3
9. Perfume appropriate amount10. Purified water remaining amount Ingredients 1 to 10 are uniformly dissolved to obtain a product.

(Formulation example 7) Foundation Formulation Formulation amount (% by weight)
1. Hermanus extract 1.0
2. Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20E.O.) 1.0
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Butyl paraoxybenzoate 0.1
10. Sodium carboxymethylcellulose 0.1
11. Bentonite 0.5
12 Propylene glycol 4.0
13. Triethanolamine 1.1
14 Methyl paraoxybenzoate 0.2
15. Magnesium phosphate ascorbate (APM) 1.0
16. Titanium dioxide 8.0
17. Talc 4.0
18. Bengala 1.0
19. Yellow iron oxide 2.0
20. Perfume appropriate amount 21. Purified water remaining

  Ingredients 2 to 9 are dissolved by heating and kept at 80 ° C. to form an oil phase. Ingredient 21 is well swollen with ingredient 10, then ingredients 1 and 11-15 are added and mixed uniformly. To this, components 16 to 19 pulverized and mixed with a pulverizer are added to obtain an aqueous phase. The aqueous phase is heated to 80 ° C., and the aqueous phase is gradually added to the oil phase to emulsify. Then, it cools with stirring, adds the component 20 at 45 degreeC, and cools to 30 degreeC to make a product.

(Formulation Example 8) Solid soap Formulation Blending amount (% by weight)
1. Hermanus extract 0.1
2. Soap base (*) 80.0
3. Glycerin 10.0
4). Sorbitol 1.0
5. Edetic acid 0.1
6). Titanium oxide 0.1
7. Perfume appropriate amount 8. Purified water Remaining amount (*) High fatty acid sodium mixture containing sodium laurate, sodium myristate, sodium palmitate, sodium stearate, sodium oleate

  All the ingredients are mixed, kneaded with a mixer and a roller, pressed into a rod-shaped molding by compression with a pudder, and then the molding is cooled and dried to obtain a product.

(Formulation Example 9) Body cleanser Formulation Formulation amount (% by weight)
1. Hermanus extract 0.005
2. Stearic acid 10.0
3. Palmitic acid 8.0
4). Myristic acid 12.0
5. Lauric acid 4.0
6). Oleyl alcohol 1.5
7). Purified lanolin 1.0
8). Palm oil fatty acid diethanolamide 1.0
9. Glycerin 10.0
10. Potassium hydroxide 6.0
11. Perfume appropriate amount 12. Preservative appropriate amount13. Metal ion sequestering agent Purified water remaining

  Ingredients 2 to 5 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. The component 10 is dissolved in an appropriate amount of the component 14 and added to the oil phase for saponification. Subsequently, components 6 to 9 are added to the saponified product, and components 1, 11 to 13 and the remaining component 14 are further added at room temperature.

(Prescription Example 10)
Hair lotion Formulation Formulation amount (wt%)
1. Hermanus extract 0.2
2. Stearic acid 5.0
3. Cetyl alcohol 5.0
4). Liquid paraffin 2.0
5. Glycerin monostearate 1.3
6). Sorbitan monooleate 1.5
7). Polyoxyethylene (10) sorbitan monooleate 0.8
8). Glycerin 6.0
9. α-Tocopheryl sodium phosphate (VEP) 0.5
10. Preservative appropriate amount11. Purified water remaining

  Ingredients 2-7 were dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 8-11 were dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase was added to the oil phase to emulsify, and the product was cooled with stirring to obtain a product.

(Prescription Example 11) Hair Tonic Formulation Blending amount (% by weight)
1. Hermanus extract 2.0
2.95% ethanol 60.0
3. Glycerin 2.0
4). Purified water remaining amount Dissolve component 1 in 2, add components 3 and 4, and mix well with stirring to obtain a product.

(Formulation Example 12) Shampoo Formulation Blending amount (% by weight)
1. Hermanus extract 0.1
2. Alkyl sulfate triethanolamine 18.0
3. Lauric acid diethanolamide 3.0
4). Methylcellulose 0.5
5. Perfume appropriate amount 6. Purified water remaining

  After component 4 was uniformly dissolved in component 6, components 1 and 2 were added, heated and dissolved at 70 to 75 ° C., component 3 was added, component 5 was added during cooling, and cooled to 30 ° C. to obtain a product.

(Formulation example 13) Bath agent Formulation Formulation amount (% by weight)
1. Hermanus extract 5.0
2. Sodium bicarbonate 50.0
3. α-Tocopheryl sodium phosphate 2.0
4). Yellow 202 No. 5 Perfume appropriate amount 6. Anhydrous sodium sulfate remaining amount Ingredients 1-6 are mixed uniformly to make a product.

(Formulation Example 14) Tablet Formulation Formulation amount (% by weight)
1. Hermanus extract 1.0
2. Dried corn starch 25.0
3. Carboxymethylcellulose calcium 20.0
4). Microcrystalline cellulose 40.0
5. Polyvinylpyrrolidone 7.0
6). Talc 3.0

  Components 1-5 are mixed, then 10% water is added as a binder and dried after extrusion granulation. Ingredient 6 is added to the molded granules, mixed and compressed into tablets. One tablet is 0.52 g.

(Formulation Example 15) Beverage Formulation Formulation amount (% by weight)
1. Hermanus extract 0.1
2. Stevia 0.05
3. Malic acid 5.0
4). Sodium ascorbate 1.0
5. Fragrance 0.1
6). Purified water remaining

  Components 1 to 5 are dissolved in a part of the purified water of component 6 with stirring. The remaining purified water of Component 6 is then added and mixed, heated to 90 ° C. and filled into a 50 mL glass bottle.

  The present invention relates to prevention, improvement, and treatment of various skin and hair disorders and damage such as seborrheic eczema, acne vulgaris, and seborrheic alopecia caused by increased or excessive sebum secretion. Can be used in the field of manufacturing pharmaceuticals, quasi-drugs, cosmetics, and foods and drinks for the purpose.

Claims (5)

A sebum synthesis inhibitor comprising, as an active ingredient, an extract of Hermanus fruit water or hot water, or a mixed solvent of water and ethanol . An agent for inhibiting differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells, which contains, as an active ingredient, an extract of Hermanus fruit water or hot water, or a mixed solvent of water and ethanol . A composition for external use for the suppression of sebum synthesis , comprising an extract of water of botanical fruit or hot water, or a mixed solvent of water and ethanol . A food or drink for sebum synthesis suppression , comprising an extract of Hermanus fruit water or hot water, or a mixed solvent of water and ethanol . The food or drink for sebum synthesis suppression according to claim 4 , wherein the food or drink is a health food, a functional food, a food for specified health use, or a dietary supplement.