JP6341661B2 - Sebum synthesis accelerator - Google Patents

Description

  TECHNICAL FIELD The present invention relates to a sebum synthesis accelerator containing an extract of Rhizopus as an active ingredient, and various compositions such as pharmaceuticals, quasi-drugs, cosmetics and foods and drinks containing the sebum synthesis accelerator.

  Sebum is synthesized by sebaceous gland cells in the sebaceous glands and secreted to the skin surface. Secreted sebum prevents the evaporation of moisture from the body by covering the skin, and has important functions such as physical and chemical stimulation from the outside, and the invasion of highly pathogenic bacteria and molds. It's done. For this reason, lack of sebum secretion in the skin causes skin problems such as dry skin and acne and various skin diseases such as sebum-deficient dermatitis and eczema (xeroderma) (Non-Patent Documents 1 and 2). ). Sebum also has a function of supplying necessary and appropriate oil to the hair, and is an effective component for maintaining the suppleness and beauty of hair.

  As described above, sebum is important for maintaining the moisture of skin and hair, but its synthesis declines with age. This is believed to be due to sebaceous gland atrophy and a decrease in sebum synthesis ability due to aging (Non-Patent Documents 3 and 4). Such skin with a reduced sebum secretion function is supplemented with oils by drugs such as creams, lotions and emulsions, quasi-drugs and cosmetics. However, these oil replenishment products temporarily stay on the skin surface, but their effects are temporary and have not led to sustained effects and fundamental improvements. In addition, although strong topical steroid drugs are effective, there are problems that various side effects such as skin atrophy, capillary vasodilation, and pigmentation are caused by long-term use.

  Until now, as a component having an action of promoting the synthesis and secretion of sebum by directly activating sebaceous gland cells, for example, γ-oryzanol (Non-patent Document 5), undecylenic acid (Patent Document 1), maltooligosaccharide (Patent Document) 2) Vitamin E derivatives (Patent Document 3), citranenin extract (Patent Document 4), etc. are known. By using these active ingredients, sebum secretion is promoted, and dry skin and acne occur. It has been reported to exert an effect on maintaining skin homeostasis. However, the effect is not satisfactory, and further development of a sebum synthesis / secretion promoter and a skin moisturizer is desired.

  Based on previous studies, it is known that sebaceous gland cells are produced by the differentiation of their progenitor cells (sebaceous gland undifferentiated cells), and sebum synthesized by sebaceous gland cells is secreted by sebaceous gland cell rupture. (Non-Patent Document 6). Therefore, in order to fundamentally regulate sebum secretion, it is necessary not only to control sebum synthesis / secretion of differentiated and sebaceous gland cells, but also to appropriately control the differentiation process starting from sebaceous gland undifferentiated cells.

  Kagamigusa (Scientific name: Ampelopsis japonica) is a vine perennial plant belonging to the genus Grapeaceae, and its blocky roots have detoxification, antipyretic, and analgesic effects, and are used in Chinese medicine for the treatment of suppuration and swelling of the skin. ing. In addition, the crayfish has been confirmed to have a collagen crosslinking inhibitory action and skin conditioning action (Patent Document 5). However, nothing has been known about the effect of promoting differentiation of sebaceous gland undifferentiated cells into sebaceous gland cells or the effect of promoting sebum synthesis by sebaceous gland cells.

Japanese Patent Laid-Open No. 4-134012 JP-A-5-294837 JP2007-99708 JP-A-4-95014 JP-A-6-65039

Ayako Yamamoto, Cosmetic Society Journal, 1991, 1 (4), 247-249 Susumu Takayasu, Fragrance Journal, 1998, 92, 10-13 Mashiko, Masaki, Journal of the Japanese Society of Skin, 1998, 98 (4), 443-452 Ayako Yamamoto, Fragrance Journal, 1994, 10, 11-17 Toshio Kobayashi et al., Skin, 1979, 21, 566-570 Hinde E., Exp Dermatology, 2013, 22, 631-637

  An object of the present invention is to provide a sebum synthesis promoter that promotes sebum synthesis in sebaceous gland cells, radically improves the state of sebum deficiency in the skin and scalp, and has a durable effect.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that the extract of crayfish promotes sebum synthesis by sebaceous gland cells as well as promotes differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells. As a result, the present invention has been completed.

That is, the present invention includes the following inventions.
(1) A sebum synthesis promoter containing an extract of Rhizopus as an active ingredient.
(2) An agent for promoting differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells, which contains an extract of crayfish as an active ingredient.
(3) A composition for external use on skin, comprising the agent according to (1) or (2).
(4) The external composition for skin according to (3), wherein the external composition for skin is a pharmaceutical product or a quasi-drug.
(5) The external composition for skin according to (3), wherein the external composition for skin is a cosmetic.
(6) Food / beverage products containing the agent as described in (1) or (2).
(7) The food or drink according to (6), wherein the food or drink is a health food, a functional food, a food for specified health use, or a dietary supplement.

  Since the sebum synthesis promoter of the present invention can promote the synthesis of sebum by sebaceous gland cells, it prevents, ameliorates or treats skin diseases such as skin and scalp bulk due to sebum deficiency and sebum deficiency (psoriasis). be able to. In addition, the extract of Kagamigusa, which is an active ingredient of the sebum synthesis promoter of the present invention, also has an action of promoting the differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells, so that sebaceous gland cells, which are sebum synthesis and secretion organs, increase. In addition, the sebum-deficient state can be fundamentally improved, and a durable effect can be obtained. Moreover, since the sebum synthesis promoter of the present invention uses a plant extract with a mild action as an active ingredient, it has no side effects and is highly safe. Therefore, it can be used with confidence in cosmetics, pharmaceuticals, quasi drugs, and food and drinks.

The present invention will be described in detail below.
The sebum synthesis promoter according to the present invention contains an extract of Rhizopus as an active ingredient. The crayfish (Ampelopsis japonica) used in the present invention is a vine perennial plant native to China belonging to the genus Grapeaceae. The rhizome of Kagamigusa is a herbal medicine called biakuren and is used for folk remedies such as swelling, bruises, and pain relief.

  In the present invention, the extract of the chamomile is a whole plant (whole plant) or an extract of a part of a plant body such as root, leaf, stem, flower, bud, fruit, seed or a mixture thereof. Root extract is preferred. In addition, these plants may be used as they are for extraction, or may be subjected to treatments such as drying, pulverization and shredding.

  The extraction method is not particularly limited, and can be performed by stirring or column extraction using water or hot water, or a mixed solvent of water and an organic solvent. As the organic solvent, alcohols, ethers, esters, and the like can be used, but water-soluble organic solvents such as ethanol, methanol, and acetone are preferable, and one or more of these may be used.

  Particularly preferred extraction solvents include water or water-ethanol mixed polar solvents. The amount of the solvent used is not particularly limited, and for example, it may be 10 times or more, preferably 20 times or more, relative to the root part (dry weight) of the above-mentioned crayfish, but it is concentrated or isolated after extraction. For convenience of operation, it is preferably 100 times or less. Moreover, although extraction temperature and time depend on the kind of solvent to be used, for example, it is 10-100 degreeC, Preferably it is 30-90 degreeC, 30 minutes-24 hours, Preferably it can illustrate 1 to 10 hours. In addition, the extract may be used as it is in the extracted solution, but if necessary, in a range that does not affect the effect, concentration (concentration by organic solvent, vacuum concentration, membrane concentration, etc.), dilution, filtration, You may use, after performing processing, such as decoloring by activated carbon, deodorizing, ethanol precipitation. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.

In the present invention, “stimulation of sebum synthesis” refers to the promotion of synthesis of lipids mainly composed of fatty acid glycerin ester in sebaceous gland cells, and promotes differentiation induction from sebaceous gland undifferentiated cells to sebaceous cells and Furthermore, it also includes the promotion of secretion of the lipids to the skin surface. Therefore, the sebum synthesis promoter of the present invention can also be used as a differentiation promoter from sebaceous gland undifferentiated cells to sebaceous cells.

  The sebum synthesis promoter of the present invention can also be used as an additive for cell culture and a research reagent for efficiently inducing differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells.

  When the sebum synthesis promoter of the present invention is administered in vivo, it can be administered as it is, but it is provided by blending it into a composition for external use with appropriate additives as long as the effects of the present invention are not impaired. It is preferable. The skin external composition of the present invention includes pharmaceuticals, quasi drugs, cosmetics and the like.

  Since the sebum synthesis promoting agent of the present invention can promote the synthesis of sebum by sebaceous gland cells, the composition for external use of the skin containing the agent has various skin disorders and Effective in preventing, ameliorating, and treating damage. Here, the skin damage or damage caused by the decrease in sebum secretion is not limited, but is sebum deficiency (xeroderma), sebum-deficient eczema, skin pruritus, hand eczema, atopic dermatitis, dry skin, cracks , Redhead dandruff, hairiness and lack of luster, scalp and hair damage and damage such as hair loss and thinning hair are also included.

  When the sebum synthesis promoter of the present invention is added to a pharmaceutical product, it should be mixed with pharmacologically and pharmaceutically acceptable additives and formulated into various preparations suitable for application to the affected area. Can do. Pharmacologically and pharmaceutically acceptable additives include excipients, thickeners, tonicity agents, pH regulators, stabilizers, preservatives, preservatives depending on the dosage form and application. , Dispersants, emulsifiers, gelling agents, pigments, fragrances and the like can be used. A form suitable for the pharmaceutical product of the present invention is an external preparation, and examples thereof include an ointment, a cream, a gel, a liquid, and a patch. The ointment refers to a homogeneous semi-solid external preparation, and includes an oily ointment, an emulsion ointment, and a water-soluble ointment. The gel is an external preparation in which a water-insoluble component hydrate compound is suspended in an aqueous liquid. The liquid preparation refers to a liquid external preparation and includes lotions, suspensions, emulsions, liniments and the like.

  When the sebum synthesis accelerator of the present invention is blended into a quasi-drug or cosmetic, the dosage form is an aqueous system, a solubilizing system, an emulsifying system, a powder system, a powder dispersion system, an oil liquid system, a gel system, or an ointment. Any of a system, an aerosol system, a water-oil two-layer system, or a water-oil-powder three-layer system may be used. In addition to the sebum synthesis accelerator, the quasi-drug and cosmetics are selected from various ingredients, additives, bases, and the like that are usually used in compositions for external use according to the type, and appropriately blended. It can be produced according to techniques known in the art. The form may be liquid, emulsion, cream, gel, paste, spray or the like. Examples of the ingredients include oils and fats (olive oil, coconut oil, evening primrose oil, jojoba oil, castor oil, hydrogenated castor oil, etc.), waxes (lanolin, beeswax, carnauba wax, etc.), hydrocarbons (liquid paraffin, squalene, Squalane, petrolatum, etc.), fatty acids (lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid etc.), higher alcohols (myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol etc.), esters (myristin) Isopropyl acid, isopropyl palmitate, cetyl octanoate, glyceryl trioctanoate, octyldodecyl myristate, octyl stearate, stearyl stearate, etc., organic acids (citric acid, lactic acid, α-hydroxyacetic acid, pyrrolidone cal Acid, etc.), sugars (maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, etc.), proteins and protein hydrolysates, amino acids and salts thereof, vitamins, plant / animal extracts, various interfaces Examples include activators, humectants, ultraviolet absorbers, antioxidants, stabilizers, preservatives, bactericides, and fragrances.

  The types of quasi-drugs and cosmetics include, for example, lotions, emulsions, gels, cosmetics, general creams, sun creams, packs, masks, facial cleansers, cosmetic soaps, foundations, funniers, bath preparations, body lotions, Examples include body shampoos, hair shampoos, hair conditioners, scalp lotions, scalp creams, hair tonics, and hair restorers.

  The content of the sebum synthesis promoter in the composition for external use of the present invention is not particularly limited as long as it is an amount capable of exerting a sebum synthesis promoting action in sebaceous gland cells as well as a differentiation promoting action from sebaceous gland undifferentiated cells to sebaceous gland cells. For example, 0.00001 to 10% by weight is preferable as the dry solid weight of the extract of the crayfish, and 0.0001 to 1% by weight is more preferable. The above amounts are merely examples, and may be appropriately set and adjusted in consideration of the type and form of the composition, the general usage amount, efficacy / effect, cost, and the like.

  Moreover, the sebum synthesis promoter of this invention can be mix | blended with food-drinks. In the present invention, the food / beverage product is used to mean including a health food, a functional food, a dietary supplement, or a food for specified health use. The form of the food or drink may be any form suitable for edible use, for example, solid, liquid, granular, granular, powder, capsule, cream, or paste.

  The types of food and drink include bread, noodles, confectionery, dairy products, processed fishery and livestock products, processed oils and fats, processed foods, seasonings, various beverages (soft drinks, carbonated drinks, beauty drinks, nutritional drinks, fruit drinks) , Milk beverages, etc.) and concentrated beverages and powders for adjustment of the beverages, but are not limited thereto.

  The food / beverage products of the present invention may be appropriately blended with additives usually used depending on the type. As the additive, any food hygiene-acceptable additive can be used. For example, sweeteners such as glucose, sucrose, fructose, isomerized liquid sugar, aspartame, stevia; citric acid, malic acid, Acidic agents such as tartaric acid; excipients such as dextrin and starch; binders, diluents, fragrances, colorants, buffers, thickeners, gelling agents, stabilizers, preservatives, emulsifiers, dispersants, suspensions Agents, preservatives and the like.

  The compounding amount of the extract of chamomilesa in the food or drink of the present invention may be any amount that can exhibit differentiation promoting action from sebaceous gland undifferentiated cells to sebaceous gland cells and sebum synthesis promoting action in sebaceous gland cells. May be set as appropriate in consideration of typical intake, form of food and drink, efficacy / effect, taste, palatability and cost.

  Hereinafter, the present invention will be described more specifically with reference to examples. However, the present invention is not limited to these.

[Example 1]
An extract of the crayfish was produced as follows.
(Production Example 1) Preparation of hot water extract of Kagamusa root 1 L of purified water was added to 100 g of Kagamusa (dried root product), extracted at 90-100 ° C for 2 hours, filtered, and the filtrate was concentrated and frozen. Drying was performed to obtain 30 g of a hot water extract of crayfish root.

(Production Example 2) Preparation of 50% ethanol extract of scallop roots 1L of 50% ethanol aqueous solution was added to 100 g of scallops (dried root product), extracted for 1 week at room temperature, filtered, and the filtrate was concentrated under reduced pressure. It was freeze-dried to obtain 27 g of a 50% ethanol extract of cyprus root.

(Manufacturing Example 3) Preparation of ethanol extract of Kagamigusa Root Add 1 L of ethanol to 100 g of Kagamigusa (dried root product), extract at room temperature for 1 week, filter, concentrate the filtrate under reduced pressure, freeze-dry and freeze it. 25 g of ethanol extract of roots was obtained.

(Manufacture example 4) Preparation of hot water extract of the crayfish whole plant 1L of purified water is added to 100 g of the cyprus grass (dry product of whole plant), extracted at 90-100 ° C for 2 hours, filtered, and the filtrate is concentrated. Then, freeze-dried to obtain 31 g of a hot water extract of the whole plant.

(Manufacture example 5) Preparation of 50% ethanol extract of Kagamigusa whole plant Add 1 L of 50% ethanol aqueous solution to 100 g of Kagamigusa (dry product of whole plant), extract for 1 week at room temperature, filter, and concentrate the filtrate under reduced pressure. By freeze-drying, 27 g of a 50% ethanol extract of the cloverfish whole plant was obtained.

(Production Example 6) Preparation of Ethanol Extract of Whole Crabgrass 1L of ethanol was added to 100g of Crabgrass (whole plant dried product), extracted for 1 week at room temperature, filtered, and the filtrate was concentrated under reduced pressure and lyophilized. As a result, 24 g of an ethanol extract of the cyprus grass was obtained.

[Example 2]
An experiment for evaluating the effect of the extract of Crayfish was performed as follows.
(Test Example 1) Evaluation of differentiation promoting effect from sebaceous gland undifferentiated cells to sebaceous gland cells The effect of the extract of Kagamigusa manufactured in Example 1 (Production Examples 1 to 6) on the differentiation efficiency from sebaceous gland undifferentiated cells to sebaceous gland cells The effect was evaluated using the expression of Pparg (Peroxisome Proliferator-Activated Receptor γ), Plin1 (Perilipin1), and Fabp4 (Fatty Acid Binding Protein 4) as specific markers for sebaceous gland cells. The specific method is described below.

5 x 10 ⁴ hamster sebaceous gland undifferentiated cells (manufactured by KURABO) cultured in hamster sebaceous gland cell growth medium (manufactured by KURABO) are seeded in 24 well dishes at a time, and then the medium is sebaceous gland cell differentiation inducing medium (manufactured by KURABO). ) To induce differentiation. At that time, the extract of Kagamigusa (Production Examples 1 to 6) produced in Example 1 was added to a final concentration of 0.001%, and the culture was continued for 6 days.

  Cells on the 6th day after differentiation induction were washed twice with PBS (−), and RNA was extracted from the cells with RNA iso Plus (manufactured by Takara). Using 2-STEP real-time PCR kit (Applied Biosystems), RNA is reverse transcribed to cDNA, then ABI7300 (Applied Biosystems) is used for real-time PCR using the above marker gene (Pparg, Plin1, Fabp4) amplification primer set (95 ° C .: 15 seconds, 60 ° C .: 30 seconds, 40 cycles) was carried out to confirm gene expression of each gene. Other operations were carried out in accordance with established methods.

(Primer set for Pparg gene)
Forward primer: 5'-ATGTCTCACAATGCCATCAGGTT-3 '(SEQ ID NO: 1)
Reverse primer: 5'-CCGCCAACAGCTTCTCCTT-3 '(SEQ ID NO: 2)
(Primer set for Plin1 gene)
Forward primer: 5'-TCTGAGCTGAAAGGCACCATCT-3 '(SEQ ID NO: 3)
Reverse primer: 5'-GATGGGCACACTGATGCTGTT-3 '(SEQ ID NO: 4)
(Fabp4 gene primer set)
Forward primer: 5'-TGGCCAAACCCATCATGAT-3 '(SEQ ID NO: 5)
Reverse primer: 5'-TGTGCTCTCTGTTCGGATGGT-3 '(SEQ ID NO: 6)
(Internal standard glyceraldehyde 3-phosphate dehydrogenase (Gapdh) gene primer set)
Forward primer: 5'-TCACATGTCGCCTGGAGAAAG-3 '(SEQ ID NO: 7)
Reverse primer: 5'-GCCTTCGGATGCCTGCTT-3 '(SEQ ID NO: 8)

  As for the differentiation induction efficiency of each cell into sebaceous gland cells, the expression level of each gene mRNA in the cells induced to differentiate without the addition of the extract of crayfish was calculated as a ratio to the expression level of Gapdh mRNA as an internal standard. The relative expression level of each gene (the expression level of each gene / the expression level of the Gapdh gene) was set to 1.0. evaluated. The results are shown in Table 1.

  As shown in Table 1, all of the extracts of Kagamusa (Production Examples 1 to 6) showed a remarkable effect of promoting differentiation induction from sebaceous gland undifferentiated cells to sebaceous gland cells.

(Test Example 2) Evaluation of the effect of promoting sebum synthesis Inoculate 5x10 ⁴ hamster sebaceous gland undifferentiated cells cultured in hamster sebaceous gland cell growth medium in a 24-well dish, and then replace the medium with a sebaceous cell differentiation-inducing medium. Thus, differentiation induction was performed. At that time, the extract of Kagamigusa manufactured in Example 1 (Production Examples 1 to 6) was added so that the final concentration was 0.001%, and the culture was continued for 12 days. After 12 days of culture, the cells were collected, washed twice with PBS (−), and the lipid synthesized in the sebaceous gland cells was measured according to a predetermined method using a lipid synthesis measurement kit (manufactured by KURABO).

  The effect of promoting sebum synthesis is the ratio of the amount of lipid synthesis in cells cultured without adding the sample to the control (1.0), and the ratio of the amount of lipid synthesis in the cells cultured with the sample added to the amount of synthesized lipid ( Control ratio) was calculated. The results are shown in Table 2.

  As shown in Table 2, all of the extracts of Kagamusa (Production Examples 1 to 6) showed a remarkable effect of promoting sebum synthesis in sebaceous gland cells.

[Example 3] Formulation example of product The formulation example of the product which mix | blended the extract of Kagamigusa manufactured in manufacture examples 1-6 is shown below.
(Formulation Example 1) Lotion Formulation Blending amount (% by weight)
1. Oyster extract 0.1
2. 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Fragrance 0.1
11. Purified water remaining

  Components 1 to 6 and 11 and components 7 to 10 are uniformly dissolved, then both are mixed and filtered to prepare a lotion.

(Formulation example 2) Cream Formulation Blending amount (% by weight)
1. Oyster extract 0.1
2. Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.2
12 Ethyl paraoxybenzoate 0.05
13.1,3-Butylene glycol 8.5
14 Purified water remaining

  Ingredients 2-9 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. Next, the aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

(Prescription Example 3) Emulsion
Formulation amount (% by weight)
1. Oyster extract 0.1
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. Purified water remaining

  Ingredients 2 to 8 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 9 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

(Formulation Example 4) Gel formulation Formulation amount (% by weight)
1. Oyster extract 0.1
2. Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4). Polyoxyethylene hydrogenated castor oil 0.1
5. Perfume proper amount 6.1,3-butylene glycol 5.0
7). Glycerin 5.0
8). Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. Purified water remaining

  Ingredients 2 to 5 and ingredients 1 and 6 to 11 are uniformly dissolved, and both are mixed to obtain a product.

(Formulation Example 5) Ointment Formulation Formulation amount (% by weight)
1. Oyster extract 2.0
2. Polyoxyethylene cetyl ether (30E.O.) 2.0
3. Glycerol monostearate 10.0
4). Liquid paraffin 5.0
5. Cetanol 6.0
6). Methyl paraoxybenzoate 0.1
7). Propylene glycol 10.0
8). Purified water remaining

  Ingredients 2 to 5 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 6 to 8 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the product is cooled to 30 ° C. with stirring to obtain a product.

(Formulation example 6) Pack
Formulation amount (% by weight)
1. Oyster extract 0.1
2. Polyvinyl alcohol 12.0
3. Ethanol 5.0
4.1,3-Butylene glycol 8.0
5. Methyl paraoxybenzoate 0.2
6). Paraoxyethylene hydrogenated castor oil (20 EO) 0.5
7). Citric acid 0.1
8). Sodium citrate 0.3
9. Perfume appropriate amount10. Purified water remaining amount Ingredients 1 to 10 are uniformly dissolved to obtain a product.

(Formulation example 7) Foundation Formulation Formulation amount (% by weight)
1. Oyster extract 1.0
2. Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20E.O.) 1.0
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Butyl paraoxybenzoate 0.1
10. Sodium carboxymethylcellulose 0.1
11. Bentonite 0.5
12 Propylene glycol 4.0
13. Triethanolamine 1.1
14 Methyl paraoxybenzoate 0.2
15. Titanium dioxide 8.0
16. Talc 4.0
17. Bengala 1.0
18. Yellow iron oxide 2.0
19. Perfume proper amount20. Purified water remaining

  Ingredients 2 to 9 are dissolved by heating and kept at 80 ° C. to form an oil phase. Swell component 10 well with component 20, then add components 1 and 11-14 and mix uniformly. To this, ingredients 15 to 18 pulverized and mixed with a pulverizer are added to obtain an aqueous phase. The aqueous phase is heated to 80 ° C., and the aqueous phase is gradually added to the oil phase to emulsify. Then, it cools with stirring, and the component 19 is added at 45 degreeC, and it cools to 30 degreeC to make a product.

(Formulation example 8) Hand cream Formulation Formulation amount (% by weight)
1. Oyster extract 0.5
2. Cetanol 4.0
3. Vaseline 2.0
4). Liquid paraffin 10.0
5. Tocopherol acetate 0.1
6). Vitamin D 0.1
7). Urea 2.0
8). Glycerin 20.0
9. Polyoxyethylene (60) glyceryl isostearate 2.5
10. Glycerol monostearate 1.5
11. Preservative 0.1
12 Perfume appropriate amount13. Purified water remaining

  Ingredients 2 to 6 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1, 7 to 11 and 13 are heated and dissolved and mixed, and kept at 75 ° C. to obtain an aqueous phase. Next, the aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 12 is added at 45 ° C., and further cooled to 30 ° C. to obtain a product.

(Formulation example 9) Solid soap Formulation Blending amount (% by weight)
1. Oyster extract 0.1
2. Soap base (*) 80.0
3. Glycerin 10.0
4). Sorbitol 1.0
5. Edetic acid 0.1
6). Titanium oxide 0.1
7. Perfume appropriate amount 8. Purified water Remaining amount (*) High fatty acid sodium mixture containing sodium laurate, sodium myristate, sodium palmitate, sodium stearate, sodium oleate

  All the ingredients are mixed, kneaded with a mixer and a roller, pressed into a rod-shaped molding by compression with a pudder, and then the molding is cooled and dried to obtain a product.

(Prescription Example 10) Body Cleaning Formulation Formulation Amount (% by weight)
1. Ragweed extract 0.2
2. Stearic acid 10.0
3. Palmitic acid 8.0
4). Myristic acid 12.0
5. Lauric acid 4.0
6). Oleyl alcohol 1.5
7). Purified lanolin 1.0
8). Palm oil fatty acid diethanolamide 1.0
9. Glycerin 10.0
10. Potassium hydroxide 6.0
11. Perfume appropriate amount 12. Preservative appropriate amount13. Metal ion sequestering agent Purified water remaining

  Ingredients 2 to 5 are dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. The component 10 is dissolved in an appropriate amount of the component 14 and added to the oil phase for saponification. Subsequently, components 6 to 9 are added to the saponified product, and components 1, 11 to 13 and the remaining component 14 are further added at room temperature.

(Formulation example 11) Hair lotion Formulation Blending amount (% by weight)
1. Ragweed extract 0.2
2. Stearic acid 5.0
3. Cetyl alcohol 5.0
4). Liquid paraffin 2.0
5. Glycerin monostearate 1.3
6). Sorbitan monooleate 1.5
7). Polyoxyethylene (10) sorbitan monooleate 0.8
8). Glycerin 6.0
9. Preservative appropriate amount10. Purified water remaining

  Ingredients 2-7 were dissolved by heating and mixed, and kept at 70 ° C. to obtain an oil phase. Ingredients 1 and 8 to 10 were dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase was added to the oil phase to emulsify, and the product was cooled with stirring to obtain a product.

(Prescription Example 12) Hair Tonic Formulation Blending amount (% by weight)
1. Oyster extract 2.0
2.95% ethanol 60.0
3. Glycerin 2.0
4). Purified water remaining

  Dissolve component 1 in 2, add components 3 and 4, and mix well with stirring to obtain a product.

(Formulation Example 13) Shampoo Formulation Blending amount (% by weight)
1. Oyster extract 0.1
2. Alkyl sulfate triethanolamine 18.0
3. Lauric acid diethanolamide 3.0
4). Methylcellulose 0.5
5. Perfume appropriate amount 6. Purified water remaining

  After component 4 was uniformly dissolved in component 6, components 1 and 2 were added, heated and dissolved at 70 to 75 ° C., component 3 was added, component 5 was added during cooling, and cooled to 30 ° C. to obtain a product.

(Formulation example 14) Bath agent Formulation Formulation amount (% by weight)
1. Oyster extract 5.0
2. Sodium bicarbonate 50.0
3. Yellow 202 No. 4 Perfume appropriate amount 5. Anhydrous sodium sulfate

  Ingredients 1-5 are mixed uniformly to make a product.

(Formulation Example 15) Tablet Formulation Formulation amount (% by weight)
1. Oyster extract 1.0
2. Dried corn starch 25.0
3. Carboxymethylcellulose calcium 20.0
4). Microcrystalline cellulose 40.0
5. Polyvinylpyrrolidone 7.0
6). Talc 3.0

  Components 1-5 are mixed, then 10% water is added as a binder and dried after extrusion granulation. Ingredient 6 is added to the molded granules, mixed and compressed into tablets. One tablet is 0.52 g.

(Formulation Example 16) Beverage Formulation Blending amount (% by weight)
1. Oyster extract 0.1
2. Stevia 0.05
3. Malic acid 5.0
4). Fragrance 0.1
5. Purified water remaining

  Components 1 to 4 are dissolved in a part of purified water of component 5 with stirring. The remaining purified water of Component 5 is then added and mixed, heated to 90 ° C. and filled into a 50 mL glass bottle.

  The present invention relates to pharmaceuticals and quasi-drugs for the purpose of prevention, improvement and treatment of various skin disorders and injuries such as sebum deficiency (dry skin disease) and sebum-deficient eczema caused by decreased sebum secretion. It can be used in the field of manufacturing cosmetics and foods and drinks.

Claims (7)

A sebum synthesis accelerator containing hot water of oysters , 50% ethanol, or an ethanol extract as an active ingredient. An agent for promoting differentiation from sebaceous gland undifferentiated cells to sebaceous gland cells, which contains hot water of Karagusa , 50% ethanol, or an ethanol extract as an active ingredient. A composition for external use for promoting sebum synthesis , comprising hot water of Kagamigusa, 50% ethanol, or an ethanol extract . The composition for external use for promoting sebum synthesis according to claim 3, wherein the composition for external use on the skin is a pharmaceutical product or a quasi-drug. The composition for external use for promoting sebum synthesis according to claim 3, wherein the composition for external use on skin is a cosmetic. A food and drink for promoting the synthesis of sebum , which comprises hot water of crayfish, 50% ethanol, or an ethanol extract . The food / beverage product for promoting sebum synthesis according to claim 6, wherein the food / beverage product is a health food, a functional food, a food for specified health use, or a dietary supplement.