KR101347910B1 - a Food Composition, a Cosmetic Composition, a Pharmaceutical Composition Comprising Supercritical Fluid Extract of Ginger and Extract of Dropwort Fermented - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a ginger supercritical extract and a fermented buttercup gourd extract as an active ingredient, and more specifically, to extract a ginger and a supercritical extract of ginger in order to maximize the anti-allergic effect of ginger. The present invention relates to a cosmetic composition or a pharmaceutical composition which is excellent in preventing and improving effects on allergic skin diseases by containing.

Description

A food composition, a cosmetic composition, a pharmaceutical composition comprising a supercritical fluid extract of ginger and extract of dropwort fermented

The present invention relates to a food composition, a cosmetic composition, and a pharmaceutical composition containing a ginger supercritical extract and a fermented parsley extract as an active ingredient.

Inflammatory skin diseases are diseases in which a series of clinical signs and symptoms, such as itching, swelling, erythema, peeling, etc., are caused by various stimulating factors causing a series of inflammatory reactions in the skin epithelium.

Inflammatory skin diseases include atopic dermatitis, contact dermatitis, seborrhoic dermatitis and acne. Atopic dermatitis is generally used in the same sense as eczema, which is an eczema-like skin lesion that occurs in people with atopic dermatitis. The cause of atopic dermatitis has not been identified to date and is known to have a genetic tendency, and now it is persuaded that it is a kind of autoimmune disease. Unlike normal eczema or dermatitis, it shows unusual symptoms and course, and 70 to 80% of pediatric eczema is present, and it occurs in adults recently.

Contact dermatitis is an inflammation of the skin caused by contact with an external substance. It has the same symptoms as acute eczema, but differs from eczema in that it occurs as a response to certain externally acting substances.

Seborrheic dermatitis is a dermatitis that occurs well in areas with high secretion of sebum, such as the head, forehead and armpits, and is also called seborrheic eczema. A lot of erythema and slender slits (dandruff) occur and appear in many 20s and 40s. Unlike ordinary eczema, the disease is caused by constitution or sebaceous abnormalities, sensitive to sunlight or heat, often worsening in spring and autumn, and easy to recur.

Acne is a chronic inflammatory disease of hair follicles and sebaceous glands, the main cause of the increase in sebum secretion, anaerobic skin flora propionibacterium acnes (Propionibacterium acnes) is the main cause. Several mechanisms may also work in combination. Sebum in the acne-prone areas is converted to testosterone (textosterone), a type of male hormone, into dihydrotestosterone, an active form, by 5 alpha-reductase. Is produced by oversecretion. The excess sebum produced above stagnates in the hair follicles and is converted into free fatty acids and various low molecular substances by lipolytic enzymes and chemotactic factors secreted by propionibacterium acne, an anaerobic bacterium that sebum occludes the hair follicles. do. This causes white blood cells to gather around the hair follicles, destroying the hair follicle wall, causing the contents of the hair follicles to leak into the dermis, causing an inflammatory reaction.

Until now, antihistamines, vitamin ointments, and corticosteroids have been mainly used for the treatment of inflammatory skin diseases. However, these drugs are often temporary and often have severe side effects.

In particular, in the case of atopic dermatitis, various 5-lipokishinase inhibitors have been proposed as candidate compounds for antiallergic agents, and chromoline alleviates the reaction of allergens with tissue mast cells. It is known that the above substances also have the disadvantage that the clinical effect is not obvious.

Acne has been used to treat acne by using an antibiotic such as erythromycin or a method of controlling sebum using estrogen, a female hormone, but there are problems associated with side effects. Vitamin A derivatives, benzoyl peroxide, salicylic acid, triclosan and the like have been used in the cosmetics for the treatment of acne, but these substances have some antibacterial effects, but have problems such as skin redness, skin sensitization, or photosensitivity.

Therefore, the inventors of the present invention are developing a composition having no side effects that can effectively prevent or treat allergic skin diseases. Ginger supercritical extract and fermented parsley extract are remarkably effective in preventing or treating allergic skin diseases compared to a single substance. The present invention has been completed by confirming that it can be used safely in a living body because it is excellent and does not exhibit toxicity.

The present invention is to provide a food composition, cosmetic composition or pharmaceutical composition for the prevention or improvement of allergic skin diseases by containing a ginger supercritical extract and fermented parsley extract as an active ingredient.

Accordingly, the present invention provides a food composition for the prevention or improvement of allergic skin diseases, which comprises a ginger supercritical extract and fermented parsley extract as an active ingredient.

Accordingly, the present invention provides a cosmetic composition for the prevention or improvement of allergic skin diseases, which comprises a ginger supercritical extract and fermented parsley extract as an active ingredient.

In the cosmetic composition according to the embodiment, the content of the active ingredient may be in the range of 0.05% to 95% by weight.

In the cosmetic composition according to the embodiment, the fermented buttercup gourd extract may be contained in an amount of 50 to 2000 parts by weight based on 100 parts by weight of the ginger supercritical extract.

In the cosmetic composition according to the embodiment, the ginger supercritical extract is a ginger powder using a supercritical carbon dioxide at a temperature of 35 to 55 ° C. and a pressure of 1 to 3 mL / min at a pressure of 100 to 400 bar for 1 to 3 hours. Can be obtained by supercritical extraction.

In the cosmetic composition according to the embodiment, the fermented buttercup gourd extract is mixed with 40 to 90% by weight of parsley and 10 to 60% by weight of sugar, fermented at 15 to 30 ℃ for 8 to 24 months, and then filtered out the fermentation broth It can be obtained by hot water extraction of buttercup foil.

In the cosmetic composition according to the embodiment, the ginger powder may have an average particle size of 50 ~ 500㎛.

In the cosmetic composition according to the embodiment, the sugar may be one selected from the group consisting of brown sugar, white sugar, syrup and honey.

In the cosmetic composition according to the embodiment, the formulation of the cosmetic composition is a basic cosmetic formulation consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek colors and eyebrow pencils; It may be any one selected from.

As a third preferred embodiment, the present invention provides a pharmaceutical composition for preventing or treating allergic skin diseases, which comprises a ginger supercritical extract and a fermented parsley extract as an active ingredient.

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Formulation of the pharmaceutical composition for the prevention or treatment of allergic skin diseases according to the embodiment, PLASTERS, lotion (LPTIONS), linings (LINIMENTS), liquid (LIQUIDS AND SOLUTIONS), aerosol (AEROSOLS) ), EXTRACTS, Ointments, FLUIDEXTRACTS, Emulsions, Emulsions, SUSPENSIONS, CAPSULES, CREAMS, Soft or hard gelatin It may be any one selected from a sustained release agent.

The present invention provides a food composition, cosmetic composition or pharmaceutical composition excellent in the prevention and improvement effect against allergic skin diseases by containing a supercritical extract of ginger and fermented parsley extract in order to maximize the anti-allergic effect of ginger. Can be.

Figure 1 is a graph showing the β-hexosaminidase release inhibitory ability of the ginger supercritical extract according to an embodiment of the present invention.
Figure 2 is a graph showing the cytotoxicity of the ginger supercritical extract according to a preferred experimental example of the present invention.
Figure 3 is a graph showing the β-hexosaminidase release inhibition ability of the fermented buttercup gourd extract according to an experimental example of the present invention.
Figure 4 is a graph showing the cytotoxicity of the fermented buttercup gourd extract according to an embodiment of the present invention.
FIG. 5 is a graph showing β-hexosaminidase release inhibitory ability (antiallergic) of a composition containing a ginger supercritical extract and a fermented parsley extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail.

First, the present invention provides a food composition for the prevention and improvement of allergic skin diseases by containing ginger supercritical extract and fermented parsley extract as an active ingredient. The food composition containing the ginger supercritical extract and fermented parsley extract of the present invention not only has a high anti-allergic effect but also no toxic effect, which is very suitable for the prevention and improvement of allergic skin diseases. The food composition may be used in the form of tea, beverages, dietary supplements, capsules and the like.

The present invention also provides a cosmetic composition for the prevention and improvement of allergic skin diseases containing ginger supercritical extract and fermented parsley extract as an active ingredient. At this time, the content of the active ingredient is preferably in the range 0.05 ~ 95% by weight relative to the total weight of the composition. In addition, the fermented buttercup gourd extract in the composition is preferably contained at 50 to 2000 parts by weight relative to 100 parts by weight of the ginger supercritical extract because the anti-allergic effect is high.

The ginger supercritical extract is obtained by extracting ginger powder by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide. In addition to removing or supplementing the flavor and flavor peculiar to ginger, it is preferable to obtain an extract with a high extraction yield and a high content of gingerol in a short time. If the respective conditions are out of range, the extraction yield is lowered or the extraction yield is no longer increased, which is economically undesirable.

In the ginger supercritical extract manufacturing method, the ginger powder has an average particle size of 50 to 500 μm, and thus the extraction speed is high.

Supercritical extraction of ginger by the above method can obtain ginger oil (ginger supercritical extract) in a yield of 2.5 to 3.0% by weight in a short time.

In addition, according to the extraction method, the ginger supercritical extract having a content of 21 to 25% by weight of gingerol, which is one of the anti-allergic active ingredients, can be obtained.

The fermented buttercup gourd extract is mixed with 40 to 90% by weight of parsley and 10 to 60% by weight of sugar, fermented at 15 to 30 ℃ for 8 to 24 months, and then by filtering the fermentation broth and extracting the remaining buttercup (by-product) by hot water You can get it. After hydrothermal extraction of the fermented buttercup gourd may be further subjected to concentration and lyophilization as necessary. According to the above method, the fermented buttercup gourd extract can be obtained with a high yield of 15-25% on average.

In the fermented buttercup gourd extract manufacturing process, the sugar may be one or more selected from the group consisting of brown sugar, white sugar, starch syrup and honey.

The ginger supercritical extract and the fermented buttercup gourd extract have antiallergic efficacy, respectively, but may show an increased antiallergic effect when the two substances are mixed.

Formulation of the cosmetic composition is a cosmetic composition consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek colors and eyebrow pencils; It may be any one selected from.

Next, the present invention provides a pharmaceutical composition for the prevention and treatment of allergic skin diseases by containing a ginger supercritical extract and fermented parsley extract as an active ingredient. The pharmaceutical composition containing the ginger supercritical extract and fermented parsley extract of the present invention has not only a high anti-allergic effect but also no toxic effect, which is very suitable for the prevention and treatment of allergic skin diseases.

Formulations of pharmaceutical compositions include PLASTERS, LOTIONS, LINIMENTS, LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, OINTMENTS, Fluid X FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, CAPSULES, CREAMS, soft or hard gelatin campels, patches, sustained release agents.

Hereinafter, the configuration of the present invention in more detail through examples, but the scope of the present invention is not limited to the following examples.

[Example]

ginger Supercritical  Preparation of extract

A supercritical fluid extractor (SFT-100XW, Supercritical Fluid Technologies, Inc., Newark, DE, USA) was used, and 18 g of ginger powder having an average particle size of 50 to 500 μm was extracted into an extraction tank having a volume of 100 mL. The extraction conditions of the samples were carried out with varying pressure at 35, 45, 55 ℃ temperature to 100, 200, 300, 400 bar, the flow rate of carbon dioxide is 2 mL / min for 2 hours in the dynamic mode to extract the container immediately I received it in (vial).

Preparation of Fermented Buttercup Gourd Extract

Wash the clean parsley harvested from Jeongdae-ri, Gachang-myeon, Daegu, Bisulsan, wash and sterilize, and mix brown sugar in a weight ratio of 1: 1, and leave it at room temperature (15 ~ 25 ℃) for 1 year Was produced to prepare a buttercup fermentation broth. Then, 1kg of the remaining by-products (minaribak) remaining after fermentation were washed with water and 8L of water was added thereto for 2 hours, followed by hot water extraction, concentration, and lyophilization to prepare 192.8g (19.3% yield) of fermented buttercup meal.

ginger Supercritical  Extract and Fermented Buttercup Extract Containing Composition Preparation

The composition of the present invention was prepared by mixing 100 μg / mL of the prepared ginger supercritical extract and 1000 μg / mL of the fermented buttercup extract.

[Experimental Method]

1. Cell line culture

RAW264.7 cell line, a murine macrophage cell line, was distributed from Korea Cell Line Bank (KCLB, Seoul, Korea), and DMEM added 10% FBS (fetal bovine serum) and 1% antibiotics (penicillin / streptomycin). The medium was passaged 2-3 times a week in a 37 ℃ incubator containing 5% CO ₂ .

Cytotoxicity against mast cells (RBL-2H3) derived from rats was measured by MTT method. The cell line RBL-2H3 cells used in this experiment were tested by subcultured through the pre-cultured through the Korea Cell Line Bank (Seoul, Korea). The medium used for cell culture was 5% CO ₂ at 37 ° C using MEM medium containing 10% FBS (fetal bovine serum) and 1% antibiotics (penicillin / streptomycin). It was used after incubating in incubator for 2-3 days.

2. Measurement of β- Hexosaminidase Release Inhibition

The mast cell line RBL-2H3 was sensitized in 1 ml (2 x 10 ⁵ cells, 24 well plates) with anti-DNP IgE (0.45 µg / ml) for 16 hours and 30 minutes before activation with DNP-BSA (10 µg / mL). 20 ml of the sample for each concentration was treated at 37 ℃. After the reaction, 25 μl of the supernatant obtained by centrifugation at 400 × g for 10 minutes at 4 ° C. was reacted with 25 μl of substrate p-nitrophenyl-N-acetyl-bD-glucosaminide at 37 ° C. for 1 hour, followed by stop solution (0.1 M NaCO / NaHCO, pH 10) 200 μl was added to stop and the absorbance was measured at 405 nm to calculate the amount of b-hexosaminidase.

[Experimental Example]

Experimental Example 1 Measurement of Cytotoxicity and Inhibition of β- hexosaminidase Release of Ginger Supercritical Extract (Ginger Oil)

Testing of the toxicity of Ginger supercritical extract against RBL-2H3 cells and the inhibitory effect of antigen-induced mast cell degranulation (β-hexosaminidase release inhibitory ability) showed no cytotoxicity in the tested concentration range, and was concentration-dependent obesity. The effect of inhibiting cell degranulation was shown (FIGS. 1 and 2).

Experimental Example 2: Measurement of Cytotoxicity and β-hexosaminidase Release Inhibition of Fermented Buttercup Extract

After fermentation, the hot water extract of Buttercup, a by-product, was found to have an effect of significantly inhibiting mast cell degranulation (β-hexosaminidase release inhibitory ability), and no cytotoxicity was observed (FIGS. 3 and 4).

Experimental Example 3: Determination of Cytotoxicity and β-hexosaminidase Release Inhibition (Antiallergic) of a Mixture of Ginger Supercritical Extract and Fermented Buttercup Extract

To find out that the mixture of ginger supercritical extract and fermented parsley extract is more effective than each single substance, they are mixed in non-toxic concentration sections, respectively, to effect the effect on the activation of mast cells (β-hexosaminidase release inhibitory ability). As a result, the ginger supercritical extract showed 33% of inhibitory activity at 100µg / mL (Fig. 1), and the fermented buttercup extract showed 76% of inhibitory activity at 1000µg / mL (Figure 3). In the mixed solution of these two substances, the inhibitory activity was 89%, confirming the synergistic effect of the inhibitory effect (FIG. 5).

As described above, the composition containing the ginger supercritical extract and the fermented parsley extract of the present invention can be seen that the skin improvement effect is superior to the case of using only each single substance.

[Manufacturing Example]

As described below, cosmetic compositions such as nourishing cream, mist and body wash containing the ginger supercritical extract obtained in Example 1 and the fermented parsley extract were prepared according to a conventional method.

Manufacturing example  1: Cream

ingredient content(%) Remarks Purified water 71.39 Solvent Isoamyl laurate 8.0 Skin conditioning agent Butylene glycol 5.0 Skin conditioning agent Glycerin 5.0 Skin conditioning agent Glyceryl Stearate 4.0 Surfactant-Emulsifying Agent Cetearyl Alcohol 2.0 Emusion Stabilizer Caprylyl glycol 0.3 Skin conditioning agent Caprylhydroxamic acid 0.1 Chelating Agent Ginger Supercritical Extract 0.06 Ginger supercritical extract, anti-allergic effect Fermented Buttercup Gourd Extract 1.8 Buttercup sugar extract, anti-allergic effect Mixed extract
(Red ginseng, evergreen mugwort, hedgehog, fennel, cypress leaves) 1.2 Skin conditioning agent Camellia oil 1.0 Skin conditioning agent Spices 0.15 -

Manufacturing example  2: mist

ingredient content(%) Remarks Purified water 67.48 Solvent Butylene glycol 2.5 Skin conditioning agent Glycerin 3.0 Skin conditioning agent ethanol 16.0 Solvent Cyclodextrin 0.1 Absorbent Caprylyl glycol 0.4 Skin conditioning agent Caprylhydroxamic acid 0.1 Chelating Agent Ginger Supercritical Extract 0.02 Ginger supercritical extract, anti-allergic effect Fermented Buttercup Gourd Extract 1.5 Buttercup sugar extract, anti-allergic effect Mixed extract
(Red ginseng, evergreen mugwort, hedgehog, fennel, cypress leaves) 8.0 Skin conditioning agent Tocopheryl Acetate 0.1 Skin conditioning agent, vitamin E derivative Niacinamide 0.3 Skin conditioning agent, Vitamin B3 Copper Tripeptide-1 0.3 Skin conditioning agent Arginine 0.2 Skin conditioning agent, amino acids

Manufacturing example  3: body Wash

ingredient content(%) Remarks Purified water 64.78 Solvent Sodium Laureth Sulfate 15.0 Surfactant-Cleansing Agent Lauryl glucoside 6.0 Surfactant-Cleansing Agent Cocamidopropyl Betaine 6.0 Surfactant-Cleansing Agent Caprylyl glycol 0.4 Skin conditioning agent Caprylhydroxamic acid 0.1 Chelating Agent Ginger Supercritical Extract 0.02 Ginger supercritical extract, anti-allergic effect Fermented Buttercup Gourd Extract 1.5 Buttercup sugar extract, anti-allergic effect Mixed extract
(Red ginseng, evergreen mugwort, hedgehog, fennel, cypress leaves) 5.0 Skin conditioning agent Laurdimonium Hydroxypropyl
Hydrolyzed Wheat Protein 0.5 Antistatic agent Spices 0.7 -

Although preferred embodiments, experimental examples, and preparations of the present invention have been shown and described above, the present invention is not limited to the above-described examples or preparations, but deviates from the gist of the present invention as claimed in the following claims. Without departing from the scope of the present invention, it will be apparent to those skilled in the art that various modifications can be made without departing from the scope of the claims.

Claims (14)

Food composition for the prevention or improvement of allergic skin diseases containing ginger supercritical extract and fermented parsley extract as an active ingredient. Cosmetic composition for the prevention or improvement of allergic skin diseases containing ginger supercritical extract and fermented parsley extract as an active ingredient. 3. The method of claim 2,
The content of the active ingredient is a cosmetic composition, characterized in that 0.05% to 95% by weight. 3. The method of claim 2,
Fermented buttercup gourd extract is a cosmetic composition containing 50 to 2000 parts by weight relative to 100 parts by weight of ginger supercritical extract. 3. The method of claim 2,
The ginger supercritical extract was obtained by extracting ginger powder by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide. Cosmetic composition characterized in that. 3. The method of claim 2,
The fermented buttercup gourd extract is obtained by mixing 40 to 90% by weight of parsley and 10 to 60% by weight of sugar, fermenting at 15 to 30 ° C. for 8 to 24 months, filtering the fermentation broth, and extracting the remaining parsley. Cosmetic composition to be. The method of claim 5,
Ginger powder is a cosmetic composition having an average particle size of 50 ~ 500㎛. The method according to claim 6,
The sugar is a cosmetic composition, characterized in that at least one selected from the group consisting of brown sugar, white sugar, starch syrup and honey. 9. The method according to any one of claims 2 to 8,
Formulation of the cosmetic composition is a cosmetic composition consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek colors and eyebrow pencils; Cosmetic composition, characterized in that any one selected from. delete delete delete A pharmaceutical composition for the prophylaxis or treatment of allergic skin diseases, comprising a ginger supercritical extract and fermented parsley extract as an active ingredient. The method of claim 13,
Formulations of pharmaceutical compositions include PLASTERS, LOTIONS, LINIMENTS, LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, OINTMENTS, Fluid X Allergy, characterized in that any one selected from (FLUIDEXTRACTS), emulsion (EMULSIONS), suspending agent (SUSPENSIONS), capsules (CAPSULES), creams (CREAMS), soft or hard gelatin campel, patch, sustained-release agent Pharmaceutical compositions for the prevention or treatment of skin diseases.

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