JP7032852B2 - Hair cosmetics - Google Patents

Description

The present invention relates to a hair cosmetic containing a novel functional material derived from a natural product and having excellent biosafety, and having a hair growth, hair nourishing effect, hair elasticity, elasticity improving effect, and scalp inflammation preventing effect. Is.

In recent years, the scalp has been damaged by various factors such as aging, stress, and ultraviolet rays, and the number of people who have hair problems not only for men but also for women is increasing. Proposed. Conventionally, as hair troubles, androgenetic alopecia (male pattern baldness) and female pattern baldness (male pattern baldness that occurred in women) have been studied, and androgens are involved in these alopecia. It has become clear that various anti-androgens and female hormone-like agents have been proposed as agents for improving hair troubles.

In recent years, bone morphogenetic protein-2 (BMP-2) has been attracting attention as a factor involved in hair growth. It is known that BMP2 is involved in the formation of hair roots and its expression is reduced by a decrease in female hormones. From this, the effects of hair growth and hair growth are expected from the components that promote the expression of BMP-2.

In order to prevent the aging and unhealthyness of the scalp as described above and maintain it in a youthful state, and based on the above-mentioned research on the mechanism of scalp damage and hair loss, various hair growth / hair nourishing agents and hair loss suppression Agents (referred to as hair growth agents and the like) have been proposed. For example, minoxidil and adenosine are known as active ingredients that exert the effects of hair growth and hair growth, and hair growth agents and the like containing these components have been proposed. However, the active ingredient blended in conventional hair growth agents and the like may cause side effects such as irritation of the scalp, and there is a demand for a functional material that is sufficiently effective and highly safe.

As a result of diligently studying the above-mentioned problems of the prior art, the present inventors have found that the extract of kudzu root containing puerarin as an active ingredient has excellent hair growth / nourishing effects, hair quality improving effects, and hair quality improving effects. The present invention has been completed by newly discovering that it has an effect of preventing / ameliorating inflammation of the scalp and that it is useful as an active ingredient of hair cosmetics.

Conventionally, it is known from Patent Documents 1 and 2 that the extract of kudzu root containing puerarin as an active ingredient has moisturizing and whitening effects, but kudzu root containing puerarin as an active ingredient. It has not been known that the extract of Puerarin can be used as an active ingredient of hair cosmetics which exerts effects such as hair growth / growth effect, hair quality improvement effect, and scalp inflammation prevention / improvement effect.

Japanese Patent Application Laid-Open No. 64-016709 Japanese Patent Application Laid-Open No. 07-157494

The present invention is a hair cosmetic containing an extract of the root of the genus Pueraria montana, which contains puerarin as an active ingredient.
In this specification, the term cosmetics is used in a broad sense to include not only so-called cosmetics but also quasi-drugs.

The present invention is a hair cosmetic containing an extract of Kudzu root of the genus Pueraria montana containing puerarin as an active ingredient, and according to the present invention, the female hormone-like action and androgen binding inhibition exhibited by the extract. Hair cosmetics that exert hair growth / nourishing effect, hair quality improving effect, and scalp inflammation prevention / improving effect based on the action, BMP-2 synthesis promoting action, degranulation suppressing action and histamine release suppressing action of epidermal cells. Fees can be provided.

Hereinafter, preferred embodiments of the present invention will be described in detail.
Further, the cosmetic according to the present invention is characterized by containing an extract of kudzu root (kakon), which is a plant of the genus Pueraria of the leguminous family (Fabaceae), which contains puerarin as a component. ..

The extract is prepared by washing the roots of kudzu, which is the extraction target site, with water in advance to remove foreign substances, then drying the roots as they are or drying them, and then chopping or crushing them if necessary, and then dipping. It can be carried out by contacting with an extraction solvent according to a conventional method such as a countercurrent extraction method or a steam distillation method. In some cases, a supercritical extraction method may be adopted.

Extraction solvents include water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; ethyl acetate, butyl acetate, methyl propionate and the like. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon-based solvents such as n-hexane, toluene and chloroform, etc., which may be used alone or in admixture of two or more. Used. In the present invention, lower alcohols or polyhydric alcohols, or water and lower alcohols or polyhydric alcohols, or water and lower alcohols or polyhydric alcohols, because they can be applied to a wide range of products and because puerarin can be obtained in a high concentration. A mixed solvent with and is preferably used.

In preparing the extract, the pH of the extract is not particularly limited, but is generally preferably in the range of pH 3-9. The pH is adjusted by adding an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, an acid adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid to the above-mentioned extraction solvent. be able to.

Extraction conditions such as extraction temperature and time differ depending on the type and pH of the solvent used, the size of the roots of waste, etc., but for example, methanol or ethanol, or a mixed solvent of water and a lower alcohol or polyhydric alcohol is extracted. In the case of the dipping method using a solvent, the extraction temperature is in the range of 0 to 80 ° C, more preferably 4 ° C to 40 ° C. The extraction time is preferably in the range of 1 hour to 7 days in the case of cold temperature extraction at 4 ° C., and is preferably in the range of 1 hour to 3 days in the case of medium temperature extraction around 40 ° C. In the case of the dipping method, the bath ratio is a weight ratio, and it is preferable that the amount of the solvent is generally 1 to 200 times, preferably 1 to 100 times the amount of the cucumber.

The kudzu root extract prepared as described above is further combined with one or more of activated carbon treatment, ion exchange resin treatment, synthetic adsorbent, silica gel, and recrystallization treatment to form puerarin. It is preferable to prepare an extract containing a high concentration of silica gel. When the treatment is performed with an ion exchange resin, it is preferable to use one having a particle size of 0.30 to 1.20. Activated carbon includes trees such as pine, bamboo, coconut husks, peach husks and other vegetable substances, as well as coal and petroleum husks as raw materials, and high-temperature carbonization that uses gas such as water vapor, carbon dioxide and air as the raw materials. Any of activated carbon obtained by subjecting it to a physical method such as a method or a chemical method such as zinc chloride and then heating it to make it porous may be used. The synthetic adsorbent is made of a nonionic resin such as a styrene / divinylbenzene copolymer or a methacrylic acid ester polymer, and has a specific surface area generally in the range of 100 to 2000 m ² / g (aromatic). Non-replacement type, etc.). For example, styrene / divinylbenzene-based Diaion HP10, 20, 21, Sepabeads SP800, SP850, SP700, SP207 (above, Mitsubishi Chemical Corporation), Amberlite XAD4, 16, Duolite S874, 877. (Rohm and Haas Co., Ltd.), styrene-based diamond HP1MG, 2MG (Mitsubishi Chemical Corporation), Amberlite XAD7 (Rohm and Haas Co., Ltd.) and the like can be mentioned. The synthetic adsorbent that can be used in the present invention is not limited to this.

Further, the extract of kudzu root may be hydrolyzed with an enzyme, an acid or an alkali at the extraction site, if necessary, prior to the extraction treatment of the present invention or in parallel with the extraction treatment. .. As a result, there is a possibility that the storage stability of the extract can be improved and the extract can be used more effectively.

In the case of hydrolysis treatment, the enzymes include proteolytic enzymes such as actinase, papaine, kimopapine or pepsin, starch degrading enzymes such as glucoamylase, α-amylase or β-amylase, and fibrous elements such as cellulase, hemicellulase or pectinase. One or more selected from any of the degrading enzymes and lipolytic enzymes such as lipase may be used, but one or more enzymes selected from those enzyme groups, respectively. May be used in combination.

The amount of the enzyme added is, for example, preferably in the range of 0.001 to 50% by weight, more preferably 0.1 to 10% by weight, based on the solid content of the roots of kudzu.

The extract prepared as described above may generally have a pH adjusted to 4 to 9 and then used as it is as a cosmetic compounding agent, or may be used as a desired concentration by concentration under reduced pressure or the like. You may. Further, the extract may be a dried product by a conventional method such as a spray drying method.

In addition, the extract prepared as described above may be stored in a refrigerator for a certain period of time and then the supernatant may be used in order to improve storage stability and the like.

The extract obtained as described above contains puerarin and its derivatives (for example, 3'-hydroxy puerarin, 3'-methoxy puerarin). In the present invention, the solid content contained in the extract is preferably 2.0 to 4.0% by weight. The amount of puerarin contained in the extract is 65% by weight or more, more preferably 70% by weight or more, and 75% by weight or more based on the weight of isoflavone in the solid content. , More preferably 80% or more by weight.

When the extract according to the present invention is blended in hair cosmetics (including quasi-drugs), for example, in the case of hair growth / hair growth cosmetics, it is generally 0.00001 to 5.0% by weight (including non-medicinal products). The solid content is% by weight, the same applies hereinafter), preferably 0.001 to 3.0% by weight. In the case of shampoo and other hair-washing cosmetics, the content is generally 0.00001 to 5.0% by weight (solid content weight%, the same applies hereinafter), preferably 0.0001 to 1.0% by weight. Is. In the case of a rinse or conditioner, it is generally 0.00001 to 5.0% by weight (solid content weight%, the same applies hereinafter), preferably 0.001 to 1.0% by weight.

Other active ingredients (hair matrix cell activator, anti-male hormone agent, blood circulation promoter, etc.) used in hair cosmetics when the extract according to the present invention is blended into hair cosmetics (including non-medicinal products). It may be blended in combination with a sebum secretion inhibitor, an anti-inflammatory agent, a hair protectant, a hair cycle growth maintainer, etc., thereby synergistic hair growth / nourishing effect, hair quality improving effect and Prevention of scalp inflammation: It can also be expected to have an improving effect.

For example, the components that can be expected to have a synergistic effect of hair growth and hair growth include minoxidyl, ciproterone acetate, pentadecanoic acid glyceride, 6-aminobenzylpurine (cytopurine), adenosine, and trans-3,4'-dimethyl3-hydroxy. Flabanone (t-flabanone), sembri extract, hinokithiol, photosensitizer, pantothenic acid and its derivatives, vitamin E and its derivatives, nicotinic acid derivatives (nicotinic acid amide, etc.), carpronium chloride, female hormones (ethynyl estradiol, estron, etc.) , Salicylic acid, potassium glycyrrhizinate (can elephant extract), hinokithiol, benzalkonium chloride, isopropylmethylphenol, l-menthol, pyridoxin hydrochloride (vitamin B6), tioxolone, camphor, resorcin, bis-type alkaloid obtained from Tamasakitsuzurafuji, Maimai Flower extract, Gentiana extract, Chamomile extract, Dutch mustard extract, Mitsuishikonbu extract, Otaneninjin extract or its fermented product, Hass seed fermented product, Ginkgo extract, Chouji extract, Amamo extract, Black soybean extract or its hydrolyzate, Takenoko extract, Any one or more of royal jelly fermented products, amino acids, vitamins and the like may be blended.

In addition to the extract, the cosmetics (including non-medicinal products) containing the extract according to the present invention include components usually used in cosmetics, such as oily components and surfactants (synthetic system, Natural products), moisturizers, thickeners, preservatives / bactericides, powder components, ultraviolet absorbers, antioxidants, pigments, fragrances and the like can be appropriately blended as needed. Further, as long as the effectiveness and features of the extract according to the present invention are not impaired, other physiologically active ingredients may be combined and blended at all.

Here, examples of the oily component include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadowfoam oil, sheer butter, tea tree oil, and avocado oil. Plant-derived oils and fats such as macadamia nut oil, carrot oil, otane carrot oil, bergamot oil, and plant-derived squalane; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; Hydrocarbons such as paraffin, vaseline, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol; myristin Examples thereof include synthetic esters such as isopropyl acid, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (octyldodecyl stearate, etc.) and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, and poly. Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, Anionic surfactants such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates; quaternary ammonium salts, primary to tertiary fatty amine salts, birds. Cationic surfactants such as alkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N, N-dialkylmorphonium salts, polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene ammoniocarboxybetaine, N-acylamidepropyl-N', N'-dimethyl-N'- An amphoteric surfactant such as β-hydroxypropylammoniosulfobetaine can be used.

Examples of the emulsifier or emulsifying aid include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, and water-soluble soybeans. Polysaccharides, soybean-derived protein-polysaccharide complex, lanolin or its derivatives, cholesterol, stevia derivatives (stevia enzyme treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and theirs. Derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented sprouted rice, lactic acid bacteria fermented grains (wheat, beans, miscellaneous grains, etc.) and the like can also be blended.

Examples of the moisturizing agent include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate and the like, as well as saccharides such as trehalose and mucopolysaccharides (eg, hyalurone). Acids and their derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acids octyldodecyl, seaweed extract, silane roots (white and white) Examples include extracts, various amino acids and their derivatives.

Examples of the thickener include brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae-derived components; silane root (white and) extract; pectin, locust bean gum, aloe polysaccharide, alkaligenes-producing polysaccharide and the like. Polysaccharides; gums such as xanthan gum, tragant gum, guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, alginic acid / methacrylic acid copolymer; hyaluronic acid And derivatives thereof; examples thereof include polyglutamic acid and derivatives thereof.

Examples of antiseptic / bactericidal agents include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, and benza chloride. Plant-derived ethanol such as luconium, salicylic acid, ethanol, undesyleneic acid, phenols, jamar (imidazodini ruurea), propanediol, 1,2-pentanediol, various essential oils, bark dry distillate, radish fermented liquid, sugar cane Alternatively, there are 1,3-butylene glycol and the like.

Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic acid anhydride, mica, nylon powder, polyethylene powder, silk powder and cellulose. There are powders of the type, grains (rice, wheat, corn, millet, etc.), beans (soybeans, red beans, etc.) and the like.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl silicate, oxybenzon, 2,4-dihydroxybenzophenone, 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tertiary butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. ..

Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate, vitamin E linoleate, etc.) and the like.

Examples of the physiologically active ingredient include placenta extract, soybean extract, yukinoshita extract, perilla extract, rice bran extract or its hydrolyzate, white mustard extract or its hydrolyzate, fermented white mustard, and shakyaku. Extract or its hydrolyzate, lactic acid bacterium fermented rice, purple kib extract, lotus seed extract or its hydrolyzate, ginseng extract or its hydrolyzate, honeybee hydrolyzate, honeybee seed fermented product, liquor lees extract or it Examples include ceramide, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract and the like. In addition, coral grass extract, rice leaf extract or its hydrolyzate, eggplant (bergamot, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolyzate, apricot fruit extract, katamen giraffe, etc. Seaweed extract, soymilk fermented product, jellyfish water, rice extract or its hydrolyzate, fermented rice extract, sprouted rice extract or its hydrolyzate, sprouted rice fermented product, damask rose flower extract, Linol Acids and their derivatives or processed products (eg, liposomeized linoleic acid, etc.), animal or fish-derived collagen and its derivatives, elastin and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloaminoamino acid derivatives, vitamin A And its derivatives, allantin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, red ginseng extract, hechima extract, anaaosa extract, peach extract, peach seed Extract, Kiwi extract, Sunflower extract, Zizyphus joazeiro extract, Paudalco bark extract, Daily extract or fermented product, Hibiscus flower extract or fermented product, Hagoromogusa extract, Cherimoya extract , Mango extract, Mangostin extract, Funori extract, Karyu tea extract, Benifuki extract, Purple orchid extract, Sansho peel or seed coat extract or hydrolyzate, Benibana flower extract, Casablanca extract, Sweet potato extract Alternatively, there are fermented products thereof, Guava leaf extract, Dokudami extract, late white yuzu extract, aloe extract, fig flower extract, apple extract and the like.

Next, the present invention will be described in more detail with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and% means all parts by weight.

Production example 1. Preparation of kudzu root extract A 1 kg of kudzu root powder was added to 10 L of ethanol for warm extraction. Next, the obtained extract was filtered and concentrated, adsorbed on a column of synthetic adsorbent, and eluted with ethanol to recover a solution containing a high concentration of puerarin. Further, by recrystallization, a concentrated solution having an increased puerarin concentration was recovered. Next, the concentrate was dried to obtain 45 g of brown powder. 960 g of a mixed solvent of water and butylene glucol was added to 40 g of this powder, and the mixture was stirred and then filtered to obtain 920 g of a dark brown transparent extract solution (solid content 3.50%).

Production example 2. Preparation of Extract of Kudzu Root 43.0 g of brown powder was obtained by the same operation as in Production Example 1 except that methanol was used in place of the extraction solvent (ethanol) used in Production Example 1. 960 g of a mixed solvent of water and butylene glucol was added to 40 g of this powder, and the mixture was stirred and then filtered to obtain 916 g of a dark brown transparent extract solution (solid content: 3.12%).

Production example 3. Preparation of extract of kudzu root By the same operation as in Production Example 1, 923 g of a brown transparent extract solution was obtained. To this solution, 6.7 g of activated carbon was added, and the mixture was stirred for 1 hour to filter the solution to obtain 880 g of a brown transparent extract solution (solid content: 3.11%).

Prescription example 1. Hair tonic for hair growth [Ingredients] part l-menthol 0.8
Extract of Production Example 1 2.0
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Ethanol 20.0
Amount of purified water totaling 100 parts The above components were sufficiently stirred and mixed to obtain a hair restorer.

Prescription example 2. Hair Tonic for Hair Growth Among the components of Formulation Example 1, a hair tonic for hair growth was obtained in the same manner as in Formulation Example 1 except that the extract of Production Example 2 was used instead of the extract of Production Example 1.

Prescription example 3. Hair Tonic for Hair Growth Among the components of Formulation Example 1, a hair tonic for hair growth was obtained in the same manner as in Formulation Example 1 except that the extract of Production Example 3 was used instead of the extract of Production Example 1.

Prescription example 4. Hair tonic for hair growth [Ingredients] Part Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol Sodium 0.02
Pyridoxine hydrochloride 0.03
Adenosine 1.0
Extract of Production Example 1 1.0
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-Arginine Appropriate amount Ethanol 20
Amount of purified water totaling 100 parts The above components were sufficiently stirred and mixed to obtain a hair restorer.

Prescription example 5. Hair Tonic for Hair Growth Among the components of Formulation Example 4, a hair tonic for hair growth was obtained in the same manner as in Formulation Example 4, except that the extract of Production Example 2 was used instead of the extract of Production Example 1.

Prescription example 6. Hair Tonic for Hair Growth Among the components of Formulation Example 4, a hair tonic for hair growth was obtained in the same manner as in Formulation Example 4 except that minoxidil was used instead of adenosine.

Prescription example 7. Hair Tonic for Hair Growth Among the components of Formulation Example 4, a hair tonic for hair growth was obtained in the same manner as in Formulation Example 4 except that 6-benzylaminopurine was used instead of adenosine.

Prescription example 8. Hair Tonic for Hair Growth Among the components of Formulation Example 4, a hair tonic for hair growth was obtained in the same manner as in Formulation Example 4 except that 2.0 parts of Panax ginseng extract was used instead of adenosine.

Prescription example 9. Hair Tonic for Hair Growth Among the components of Formulation Example 4, a hair tonic for hair growth was obtained in the same manner as in Formulation Example 4, except that 0.3 part of Sinomenium acutum root extract was used instead of adenosine.

Prescription example 10. Hair cream [A component] Part Liquid paraffin 15.0
Vaseline 15.0
Sarashi Beeswax 2.0
Preservative 0.1
Fragrance 0.1
[B component]
Extract of Production Example 1 1.0
Bamboo shoot skin extract 0.3
Brown algae extract 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Glycerin 5.0
1,3-butylene glycol 2.0
Polyoxyethylene hydrogenated castor oil 3.0
Chelating agent 0.1
Dye 0.01
Amount of purified water totaling 100 parts [C component]
Caustic soda 0.05
After each of the above A component and B component was heated and dissolved at 80 ° C. or higher, the A component was added to the B component while stirring, and the mixture was emulsified using a homogenizer. After cooling this to 30 ° C., the C component was added and further stirred and mixed to obtain a milky lotion.

Prescription example 11. Hair shampoo [A component] Part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) Alkyl Ether Sodium Sulfate 20.0
Betaine Lauryldimethylaminoacetic Acid 10.0
Coconut oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
The total amount of purified water is 100 parts. After heating and uniformly dissolving the A component and the B component, the B component was added to the A component, and the mixture was continuously stirred and cooled to room temperature to obtain a hair shampoo.

Prescription example 12. Hair shampoo A hair shampoo was obtained in the same manner as in Formulation 11 except that the extract of Production Example 2 was used instead of the extract of Production Example 1 among the components of Formulation Example 11.

Prescription example 12. Hair shampoo A hair shampoo was obtained in the same manner as in Formulation 11 except that the extract of Production Example 3 was used instead of the extract of Production Example 1 among the components of Formulation Example 11.

Example 13. Hair rinse [A component] Part Polyoxyethylene (10) Hardened castor oil 1.0
Distearyl dimethylammonium chloride 1.5
Stearyl Trimethylammonium Chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[B component]
Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
The total amount of purified water is 100 parts. After heating and uniformly dissolving the A component and the B component, the B component was added to the A component, and the mixture was continuously stirred and cooled to room temperature to obtain a hair rinse.

Prescription example 14. Hair rinse A hair rinse was obtained in the same manner as in Formulation 13 except that the extract of Production Example 2 was used instead of the extract of Production Example 1 among the components of Formulation Example 13.

Prescription example 15. Hair rinse A hair rinse was obtained in the same manner as in Formulation 13 except that the extract of Production Example 3 was used instead of the extract of Production Example 1 among the components of Formulation Example 13.

Test example 1. Quantitative test of puerarin 0.1 g of each of the extract according to Production Example 1 of the present invention and the commercially available kudzu root extract (commercially available product of absolute ethanol extract, hereinafter referred to as "comparative product 1"). It was dissolved in a 70% aqueous ethanol solution to prepare a sample solution. Separately, 10 mg of Puerarin standard reagent (manufactured by Tokyo Chemical Industry Co., Ltd., purity 98% or more) was accurately weighed and dissolved in a 70% ethanol aqueous solution to prepare a standard solution. The sample solution and standard solution were analyzed using liquid chromatography (Alliance HPLC system, manufactured by Waters) under the following conditions. Detection is an ultraviolet absorptiometer (measurement wavelength: 254 nm), column is COSMOSIL 5C18MS-II (4.6 mm ID x 250 mm, manufactured by Nakaraitesk), and the mobile phase liquid is acetonitrile: water: acetic acid = 15: 85: 0.1. The mixed solution and the mixed solution of acetonitrile: water: acetic acid = 35: 65: 0.1 were analyzed at a flow rate of 1.0 mL / min by controlling the concentration gradient. The amount of puerarin was quantified from the peak area area of the obtained standard solution and the peak area area of puerarin detected in the sample solution. The amount of puerarin was calculated by the concentration (%) in the solid content of the extract or comparative product 1 according to Production Example 1 of the present invention and the ratio (%) to the total amount of solid content.

The results of Test Example 1 are shown in Table 1.
[Table 1]

As shown in Table 1, it was confirmed that the extract according to Production Example 1 of the present invention contained a high concentration of puerarin. It was also confirmed that in the extract according to Production Example 1 of the present invention, the ratio of total isoflavone: puerarin in the solid content was 1: 0.8.

Test example 2. Evaluation test of female hormone-like action 4 × 10 ³ cells / hole seeded 37 in 96-well microplates containing 10% FBS (charcoal dextrin treatment) -containing Dalveco modified Eagle minimum essential medium for human breast cancer cells MCF-7. After pre-culturing under the conditions of ° C. and 5.0% CO ₂ for 1 day, the extract (sample solution) according to Production Example 1 of the present invention and Comparative Product 1 were added to the medium, respectively. Here, the sample solution and Comparative Product 1 were prepared so that the final concentration as a solution was 0.5% and 1.0%, respectively, with respect to the total amount of the medium. After culturing for 5 days, the supernatant was discarded, 100 μL / hole of hoechst33342 reagent diluted 100-fold with PBS (−) was added, and the mixture was incubated at 37 ° C. for 1 hour, and the DNA was fluorescently stained. Then, the fluorescence intensity (excitation: 355 nm, radiation: 460 nm: fluorescent microplate reader (Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)) was measured to determine the amount of DNA. The group to which the same operation was performed for the group to which 50BG was added instead of the sample solution and the comparative product 1 was used as a control, and the fluorescence of each sample-added group with respect to the fluorescence intensity (DNA amount) obtained here was used. The relative value of intensity was calculated and used as the cell proliferation rate (%). A similar test was also performed when 0.1 nM estradiol was added as a positive control instead of the sample solution in order to confirm whether the test system was functioning normally.

The results of Test Example 2 are shown in Table 2.
[Table 2]

As shown in Table 2, the extract according to Production Example 1 of the present invention was shown to have a significantly superior female hormone-like action in a concentration-dependent manner, whereas the comparative product 1 had a female hormone. No similar action was confirmed.

Test example 3. Evaluation test of androgen binding inhibitory effect Mice-derived breast cancer cell SC-3 showing androgen-dependent growth was seeded in 96-well microplates containing 2% FBS-containing eagle minimum essential medium at 5 × 10 ³ cells / hole at 37 ° C. After pre-culturing under the conditions of 5.0% CO ₂ for 1 day, the extract according to Production Example 1 of the present invention was added so as to have a concentration of 0.5% and 1.0% (as a solution). It was prepared using HMB medium (HAM medium: Eagle medium = 1: 1) and replaced with the medium of the cell culture system. In addition, 10 nM DHT (dihydrotestosterone (highly active androgen)) was added in combination with the sample. The cells were cultured under the same conditions for another 3 days. Next, the medium was removed, 0.03% MTT was added and the mixture was kept at 37 ° C. for 1 hour, and then the formed formazan was extracted with isopropanol and used with a microplate reader (Model 680, manufactured by Biorad). The MTT value was measured at a wavelength of 570-630 nm. In the case of no sample addition (Control) in which 50 BG is added instead of the sample solution, the same operation as above is performed, and the relative value of the MTT value at the time of adding each sample to the MTT value obtained here is obtained, and SC- The proliferation rate (%) of 3 cells was used. The same test was also performed when 0.01 mM cyproterone acetate was added as a positive control instead of the sample solution in order to confirm whether the test system was functioning normally.

The results of Test Example 3 are shown in Table 3.
[Table 3]

As shown in Table 3, it was shown that the extract according to Production Example 1 of the present invention has a significantly excellent androgen binding inhibitory action in a concentration-dependent manner.

Test example 4. Evaluation test of BMP-2 synthesis promoting effect 1.2 × 10 ⁴ cells / hole were seeded on a 96-well microplate containing serum CSC medium, and the conditions were 37 ° C. and 5.0% CO ₂ . After pre-culturing underneath for 1 day, the extract according to Production Example 1 of the present invention was prepared and added using a serum-free medium so as to have a concentration of 0.5% and 1.0% (as a solution). .. The cells were cultured under the same conditions for another 2 days. Next, each culture supernatant was taken, and BMP-2 in the culture supernatant was measured using Human / Mouse / Rat BMP-2 Quantikine ELISA KIT (R & D Systems, Inc.). In the case of no sample addition (Control) in which 50 BG is added instead of the sample solution, the same operation as above is performed, and the relative value of the BMP-2 amount at the time of adding each sample to the BMP-2 amount obtained here is calculated. It was determined and used as the BMP-2 synthesis promotion rate (%). Further, in order to confirm whether the test system is functioning normally, the same test was performed when 1 nM estradiol was added as a positive control instead of the sample solution.

The results of Test Example 4 are shown in Table 4.
[Table 4]

As shown in Table 4, it was shown that the extract according to Production Example 1 of the present invention has a significantly excellent concentration-dependent BMP-2 synthesis promoting action.

Test example 5. Evaluation test of inhibitory effect on degranulation Human basophil cells (KU812-F) were suspended in RPMI minimum essential medium containing 10% FBS, seeded in 1 × 10 ⁵ cells on a 96-well plate, and cultured at 37 ° C for 24 hours. did. Releasing buffer [117mM NaCl, 5.4mM KCl, 2.0mM CaCl, 0.8mM DDL, 5.6mM D-glucose, 25mM HEPES (2- [4- (2-hydroxyethyl)) -1-Piperaginyl] ethanesulphonic acid), 1 mg / mL BSA / pH 7.7] 200 μL / well. An extract (sample solution) according to Production Example 1 of the present invention and a releasing buffer solution mixed with Comparative Product 1 were added to the wells, respectively. Here, the sample solution and Comparative Product 1 were prepared by mixing with a releasing buffer solution so that the final concentrations of the solution as a solution were 0.25% and 0.5%, respectively, with respect to the entire solution. To further induce degranulation in each well, 200 μg / mL compound 48/80 (compound 48/80) / 100 μL of releasing buffer was added and incubated at 37 ° C. for 1 hour. In addition, two test groups were provided in which a releasing buffer solution containing a 50% 1,3-butylene glycol aqueous solution was added instead of the sample solution, and only the releasing buffer solution was placed in one test group (blank) and the other test group was provided. To the test group (control), 100 μL of each of the same compound 48/80 / releasing buffer solution for degranulation as described above was added, and the mixture was also incubated at 37 ° C. for 1 hour. After induction of degranulation, 100 μL of cell supernatant was dispensed into another 96-well microplate to measure the amount of histamine released extracellularly. The amount of histamine was measured as follows. That is, 20 μL of 1N NaOH was added to 100 μL of each cell supernatant taken on a separate plate. After the addition, 5 μL of 10 mg / mL o-phthalaldehyde was added and reacted at room temperature for 4 minutes. After the reaction, 10 μL of 3N HCl was added, and the fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescent plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems), and this was used as the amount of histamine.

The results of Test Example 5 are shown in Table 5.
[Table 5]

As shown in Table 5, the extract according to Production Example 1 of the present invention was shown to have a significantly excellent concentration-dependent degranulation inhibitory effect, and this effect was remarkable as compared with Comparative Product 1. Met.

Test Example 6. Evaluation test of histamine release inhibitory effect of epidermal cells Prepare normal human epidermal keratinized cells with HuMedia-KB2 (registered trademark) (manufactured by Kurabo) containing a growth additive to 1 × 10 ⁵ cells / mL, and prepare a 24-hole microplate. 1 mL was seeded and cultured at 37 ° C. under 5% carbon dioxide gas and saturated steam. After 24 hours, a culture solution containing the extract (sample solution) according to Production Example 1 of the present invention was additionally added and cultured. Here, the sample solution was prepared so that the final concentration as a solution was 0.5% and 1% with respect to the total amount of the culture solution. In addition, a test group to which a culture solution containing 50% 1,3-butylene glycol was added was set as a control. After 24 hours, it was irradiated with UV (30 mJ) and cultured for another 24 hours. After culturing, 100 μL of the supernatant was dispensed into a 96-well microplate in order to measure the amount of histamine, and the cell activity of the cells was determined using the MTT reduction method. The amount of histamine was measured as follows. That is, 20 μL of 1N NaOH was added to 100 μL of each cell supernatant taken on a separate plate. After the addition, 5 μL of 10 mg / mL o-phthalaldehyde was added and reacted at room temperature for 4 minutes. After the reaction, 10 μL of 3N HCl was added, and the fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems), and this was used as the amount of histamine. The cell activity was measured as follows. After adding 0.03% MTT and keeping at 37 ° C. for 1 hour, the formed formazan was extracted with isopropanol, the solution was transferred to a 96-well microplate, and a microplate reader (Model 680, manufactured by Biorad) was used. The MTT value was measured at a wavelength of 570-630 nm. Using the obtained values, the amount of histamine per cell activity was determined.

The results of Test Example 6 are shown in Table 6.
[Table 6]

As shown in Table 6, it was shown that the extract according to Production Example 1 of the present invention has a remarkably excellent concentration-dependent inhibitory effect on the release of histamine from epidermal cells.

Claims (1)

A hair cosmetic containing an extract of kudzu root, wherein the solid content weight of puerarin is 65% by weight or more with respect to the solid content weight in the extract.