JP2018138533A - Hair Cosmetics - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide novel hair cosmetics which consist of a naturally occurring component having excellent biosafety and which compound with a hair cosmetic (quasi drugs are also comprised).SOLUTION: The present invention provides a hair cosmetic containing as an active ingredient inulin, caffeic acid and a derivative thereof, quinic acid, and a plant or an extract thereof comprising at least one or more of inulin, quinic acid, caffeic acid and a derivative thereof.EFFECT: The hair cosmetic has a XVII type collagen synthetic promoting effect, and has an excellent hair restoration/hair growing effect.SELECTED DRAWING: None

Description

  The present invention relates to a hair cosmetic composition containing a novel functional material excellent in biological safety derived from a natural product, and in particular, having hair growth and nourishing effects.

  In recent years, due to various factors such as aging, stress or ultraviolet rays, the scalp has been damaged, and not only men but also women have problems with hair. Corresponding to this, various hair cosmetics are available. Proposed. Conventionally, studies on male pattern alopecia (major alopecia) and female pattern alopecia (male pattern alopecia in women) have been conducted as hair problems, and male hormones are involved in these alopecia As a result, various anti-androgenic hormones and female hormone-like agents have been proposed as agents for improving hair troubles.

  In order to prevent the aging and unhealth of the scalp as described above and keep it in a youthful state, various hair-growth / hair-restoring agents and hair loss inhibitors are also based on the research on scalp damage and hair loss mechanisms described above. An agent (referred to as a hair restorer or the like) has been proposed. For example, minoxidil and adenosine are known as active ingredients that exhibit hair-growth / hair-growth effects, and hair-growth agents containing these ingredients have been proposed. However, active ingredients blended in conventional hair restorers and the like may cause side effects such as irritation of the scalp, and there is a need for functional materials that are sufficiently effective and highly safe.

  As a result of intensive studies on the above problems of the prior art, the present inventors have found at least one of inulin, quinic acid, caffeic acid (caffeic acid) or a derivative thereof, or inulin, quinic acid, caffeic acid and a derivative thereof. It has been found that plant extracts containing sucrose have an action of promoting XVII type collagen synthesis, and also have excellent hair growth and hair restoration effects, and are useful as active ingredients in hair cosmetics. The present invention has been completed.

  Conventionally, the technique which uses inulin, caffeic acid, or its derivative as an active ingredient of cosmetics is known by patent documents 1-3, for example. However, in hair cosmetics wherein plant extracts containing at least one of inulin, quinic acid, inulin and caffeic acid or derivatives thereof and at least one of inulin, quinic acid, caffeic acid and derivatives thereof exhibit hair-growth / hair-growth effects. It has not been known that it can be used as an active ingredient.

JP 07-238037 A Special table 2004-501175 JP 2004-501176 A

  As a result of intensive studies on the above-described problems of the prior art, the present inventors have extracted plant extracts containing at least one of inulin, quinic acid, caffeic acid or derivatives thereof, or inulin, quinic acid, caffeic acid and derivatives thereof. The present invention completes the present invention by newly discovering that the product has an action of promoting the synthesis of collagen type XVII, and also has an excellent hair growth / hair restoration effect, which is useful as a composition for hair cosmetics. It came to.

The present invention is a hair cosmetic comprising inulin, a plant containing the same, or an extract thereof as an active ingredient.
The present invention is a hair cosmetic comprising inulin and quinic acid or caffeic acid or a derivative thereof as active ingredients.
The present invention is a hair cosmetic comprising, as an active ingredient, a plant containing inulin and quinic acid, caffeic acid or a derivative thereof, or an extract thereof.
The present invention promotes the production of type XVII collagen comprising, as an active ingredient, a plant containing at least one of inulin, quinic acid, caffeic acid or derivatives thereof, or inulin, quinic acid, caffeic acid and derivatives thereof, or an extract thereof. It is an agent.

  The present invention is a hair cosmetic comprising as an active ingredient a plant containing at least one of inulin, quinic acid, caffeic acid or a derivative thereof, or inulin, quinic acid, caffeic acid and a derivative thereof, or an extract thereof. In addition, it has an action of promoting XVII type collagen synthesis, and also has an excellent hair growth and nourishing effect.

  In the present invention, inulin is a polysaccharide in which D-fructofuranose repeats β-2 → 1 bonds and 1 mol of D-glucose is bonded to the terminal.

  In the present invention, caffeic acid is 3,4-dihydroxycinnamic acid. Examples of caffeic acid derivatives include chlorogenic acid (3-caffeoylquinic acid). Chlorogenic acid is a compound having a structure in which the carboxyl group of caffeic acid is dehydrated and condensed with the hydroxy group at the 3-position of quinic acid. Examples of other caffeic acid derivatives include 4-caffeoylquinic acid, 3,4-dicaffeoylquinic acid, and caffeoyl glucose. Quinic acid is 1,3,4,5-tetrahydroxycyclohexanecarboxylic acid and is known to be contained in plants as a constituent component such as chlorogenic acid.

  In the present invention, inulin, quinic acid, caffeic acid and derivatives thereof may be commercially available products or extracts obtained by extraction from natural products such as plants. Further, the plant extract may be subjected to treatments such as separation, purification, and concentration to increase the concentration of inulin, quinic acid, caffeic acid and / or a derivative thereof.

  Examples of plants that can be used in the present invention include the following. That is, examples of the plant containing inulin include chrysanthemums, katsumo, thistle, chicory, agave, dahlia, artichoke, onion, garlic, and leek. Examples of plants containing quinic acid include quina bark, coffee, apple, peach and the like. In addition, examples of plants containing caffeic acid or derivatives thereof include solanaceous, sericid, and asteraceae plants, apples, pumpkins, sweet potatoes, and coffee. In the present invention, a plant containing inulin and quinic acid, caffeic acid and / or a derivative thereof is more preferable, for example, burdock of Asteraceae plant. Examples of burdocks containing a large amount of inulin and caffeic acid derivatives (chlorogenic acid) include Utakin Burdock.

  The extract is prepared by washing the plant to be extracted beforehand with water if necessary to remove foreign substances, and then leaving it as it is or drying, and further chopping or pulverizing it if necessary, followed by a dipping method or countercurrent flow. It can carry out by making it contact with an extraction solvent in accordance with conventional methods, such as an extraction method and a steam distillation method. In the present invention, a supercritical extraction method may be employed.

  The extraction solvent used for the extract treatment includes water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; ethyl acetate and butyl acetate. And esters such as methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene and chloroform. A mixture of two or more types is used. In the present invention, it is more preferable to use two kinds of mixed solvents having different polarities from the viewpoint that inulin and caffeic acid or a derivative thereof can be obtained at a high concentration.

 In preparing the extract, the pH of the extract is not particularly limited, but it is generally preferably in the range of pH 3-9. The pH is adjusted by blending an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid into the extraction solvent. be able to.

  Extraction conditions such as extraction temperature and time vary depending on the type and pH of the solvent used, the size of the plant material, and the like. For example, methanol or ethanol, or a mixed solvent of water and lower alcohol or polyhydric alcohol is used as the extraction solvent. In the case of the immersion method, the extraction temperature is in the range of 0 to 80 ° C. In the case of cold extraction at 4 ° C., the extraction time is preferably in the range of 1 hour to 7 days, and in the medium temperature extraction around 40 ° C., the extraction time is preferably in the range of 1 hour to 3 days. In the case of high-temperature extraction at 80 ° C., the range is preferably 1 hour to 24 hours. In the case of the immersion method, the bath ratio is a weight ratio, and the solvent is generally in the range of 1 to 200 times, preferably 1 to 100 times the amount of the plant material.

  The plant extract prepared as described above is further combined with one or more of activated carbon treatment, ion exchange resin treatment, synthetic adsorbent, silica gel, and recrystallization treatment to produce inulin and quinic acid. Alternatively, an extract containing a high concentration of at least one of caffeic acid and derivatives thereof may be prepared. Activated carbon is not only plant materials such as pine trees, bamboo, coconut shells, walnut shells, etc., but also carbonaceous, petroleum, etc. as raw materials, and high temperature carbonization using gas such as water vapor, carbon dioxide, air etc. as those raw materials Any of activated carbon obtained by applying a physical method such as a chemical method or a chemical method such as zinc chloride and then heating and heating to a porous chemical method may be used. Examples of the synthetic adsorbent include nonionic resins such as styrene / divinylbenzene copolymer and methacrylic acid ester polymer.

  The following amounts are preferable as the weight% of inulin, quinic acid, caffeic acid or a derivative thereof contained in the extract obtained as described above. In the present invention, the solid content of inulin contained in the extract is preferably 20 to 90% by weight. The solid content of quinic acid, caffeic acid or a derivative thereof contained in the extract is preferably 0.001 to 5.0% by weight of the solid content. Further, the amount of inulin in the hair cosmetic containing the extract is preferably 0.1 to 1.0% by weight of the solid content, and the solid content of quinic acid, caffeic acid or a derivative thereof is 0% by weight of the solid content. 0.0001 to 0.02% by weight is preferred.

  In the present invention, when inulin and quinic acid or caffeic acid or a derivative thereof are included, the relative ratio (weight ratio) between inulin and quinic acid or caffeic acid or a derivative thereof for hair cosmetics is 10: 1 to 99. : 1.

  Inulin or a derivative thereof, quinic acid or caffeic acid or a derivative thereof, and a plant extract according to the present invention have an action of promoting collagen type XVII synthesis, as described later, and also synthesize marker proteins of hair follicle stem cells. It is suggested to promote. By these actions, hair follicle stem cells that decrease with aging can be maintained in a healthy state, and the start of the hair cycle can be promoted.

  When blending the extract according to the present invention into a hair cosmetic (including quasi-drugs), for example, if it is a hair-growth / hair-raising cosmetic, it is generally 0.00001 to 5.0% by weight ( % By solid content, the same applies hereinafter), preferably 0.001 to 3.0% by weight. Further, in the case of a hair-washing cosmetic such as shampoo, it is generally 0.00001 to 5.0% by weight (solid content% by weight, hereinafter the same), preferably 0.0001 to 1.0% by weight. It is. In the case of a rinse or a conditioner, it is generally 0.00001 to 5.0 wt% (solid content wt%, hereinafter the same), and preferably 0.001 to 1.0 wt%.

  When the inulin, quinic acid, caffeic acid or derivatives thereof according to the present invention or at least one plant extract thereof is blended in hair cosmetics (including quasi-drugs), it is used for hair cosmetics. Other active ingredients (hair matrix cell activator, anti-androgen hormone agent, blood circulation promoter, sebum secretion inhibitor, anti-inflammatory agent, hair protecting agent, hair cycle growth maintaining agent, etc.) In this way, a synergistic hair-growth / hair-restoration effect, a hair quality-improving effect, and scalp inflammation prevention: an improving effect can also be expected.

  For example, components that can be expected to have a synergistic effect on hair growth and nourishing include minoxidil, cyproterone acetate, pentadecanoic acid glyceride, 6-aminobenzylpurine (cytopurine), adenosine, trans-3,4'-dimethyl 3-hydroxy Flavanone (t-flavanone), assembly extract, hinokitiol, photosensitizer, pantothenic acid and its derivatives, vitamin E and its derivatives, nicotinic acid derivatives (nicotinic acid amide, etc.), carpronium chloride, female hormones (ethinylestradiol, estrone, etc.) , Salicylic acid, dipotassium glycyrrhizinate, hinokitiol, benzalkonium chloride, isopropylmethylphenol, l-menthol, pyridoxine hydrochloride (vitamin B6), thioxolone, camphor, resorcin, tamasakitsu Extract of Root, Bis-type alkaloids obtained from Shirasu, Rhizome extract, Licorice extract, Maimai flower extract, Chamomile extract, Fermented royal jelly, Fermented lotus seed, Ginkgo biloba extract, Pardarco bark extract, Gentian extract, Panax ginseng Extract, soymilk fermented liquid, black soybean hydrolysate extract, rhododendron extract, bamboo shoot extract, kudzu root extract, beetroot extract, clove extract, collagen, amino acids, vitamins, etc., any one or more of them May be blended.

  In particular, “Dipotassium glycyrrhizinate, licorice extract, Pardarco bark extract or gentian extract” that suppresses inflammation of the scalp, “Isopropylmethylphenol” that suppresses inflammation of the scalp and has a bactericidal effect, Root extract or bis-type alkaloid obtained therefrom, “Ginseng extract” having blood flow promoting effect and sebum secretion inhibiting effect, “Sembly extract” having blood circulation promoting effect, “Soy milk fermentation solution” having sebum oxidation inhibiting effect, At least of “Acanthus extract” that restores the barrier function of the scalp, “Bamboo shoot extract or kuzu root extract” that promotes the transition to the hair growth stage, “Mitsuishi kombu extract” that inhibits hair loss, and “Collagen” that inhibits scalp drying It is preferable to use in combination with one or more.

  In addition to the above-mentioned components, hair cosmetics (including quasi-drugs) containing the extract according to the present invention usually include components used for hair cosmetics (including quasi-drugs), for example, Oily components, surfactants (synthetic and natural products), moisturizers, thickeners, antiseptics / bactericides, powder components, UV absorbers, antioxidants, chelating agents, ph adjusters, dyes, fragrances, etc. Can be appropriately blended as necessary. Moreover, as long as the effectiveness and characteristics of the active ingredient according to the present invention are not impaired, it may be combined with other physiologically active ingredients.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Macadamia nut oil, carrot oil, ginseng oil, bergamot oil, plant-derived oils such as squalane; animal-derived oils such as mink oil, turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; Hydrocarbons such as paraffin, petrolatum, paraffin wax, squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol; myristin Isopropyl acid, palmitic acid Isopropyl, butyl oleate, 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Anions such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates Surfactant; quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers or emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen hydrolyzate, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

  Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components; silane root (white) extract; pectin, locust bean gum, aloe polysaccharides, alkaligenes-producing polysaccharides, etc. Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Plant-derived ethanol such as ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), propanediol, 1,2-pentanediol, various essential oils, dry bark, radish fermented liquor, sugarcane Alternatively, there is 1,3-butylene glycol.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powder, cereal (rice, wheat, corn, millet, etc.) powder, legume (soybean, red bean, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

  Examples of chelating agents include trisodium ethylenediaminehydroxyethyl triacetate, edetic acid or salts thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, tetrasodium hydroxyethanediphosphonate, and the like.

  Examples of the pH adjuster include citric acid or a salt thereof, lactic acid or a salt thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide and the like.

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, Murasakixikib extract, Lotus seed extract or hydrolyzate thereof, Participant extract or hydrolyzate thereof, pearl barley hydrolysate, barley seed fermented product, sake lees extract or ceramide contained therein Fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, rice leaf extract or hydrolyzate thereof, eggplant (bergamot, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolyzate, apricot fruit extract, seaweed extract such as catamen giraffe, jellyfish Water, rice extract or hydrolyzate thereof, rice fermented extract, germinated rice extract or hydrolyzate thereof, germinated rice fermented product, damask rose flower extract, linoleic acid and its derivatives or extracts (eg, liposome formation) Linoleic acid etc.), animal or fish derived collagen and derivatives thereof, elastin and derivatives thereof, glycyrrhetinic acid and derivatives thereof, t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, diisopropylamine dichloroacetate, γ-amino-β -Hydroxybutyric acid, carrot extract, red ginseng extract, loofah extract, anaaosa extract, peach extract, peach extract, kiwi extract, sunflower seed or bud extract, Zizyphus joazeiro extract, camellia (Daily) Extract or fermented product, Hibiscus flower extract or fermented product, Hago Lomogusa extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, red rich extract, purple orchid extract, extract of yam peel or seed coat or hydrolysate, safflower flower extract, Examples include Casablanca extract, sweet potato extract or fermented product thereof, guava leaf extract, dokudami extract, evening birch extract, aloe extract, fig flower extract, apple extract and the like.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

  An example of producing an extract of burdock belonging to the genus Burdaceae as a plant extract containing inulin or a derivative thereof and a derivative of caffeic acid (chlorogenic acid) is shown below.

Production Example 1 Preparation of burdock extract (1)
Purified water (50 g) and ethanol (50 g) were added to 10 g of dried burdock roots and immersed at 50 ° C. This was filtered to obtain 90 g of a brown transparent burdock root extract solution (solid content concentration 1.54%). The obtained extract solution was concentrated to obtain 13.5 g of a concentrated solution.

Production Example 2 Preparation of burdock extract (2)
Purified water (50 g) and 1,3-butylene glycol (50 g) were added to 10 g of dried burdock root and immersed at 40 ° C. This was filtered to obtain 90 g of brown transparent burdock root extract (solid content concentration 1.62 g). The obtained extract solution was concentrated to obtain 13.5 g of a concentrated solution.

Production Example 3 Preparation of burdock extract (3)
Purified water (50 g) and 1,2-pentanediol (50 g) were added to 10 g of dried burdock root and immersed at 40 ° C. This was filtered to obtain 90 g of a brown transparent burdock root extract (solid content concentration: 1.59 g). The obtained extract solution was concentrated to obtain 13.5 g of a concentrated solution.

  It was confirmed by a conventional method that the extracts of Production Examples 1 to 3 contain inulin and chlorogenic acid. That is, the detection of chlorogenic acid was confirmed by using the foreign thiocult method (colorimetric method), and the inulin was confirmed by using the DNS method (colorimetric method) for detecting reducing sugars.

  In addition, the plant extract which concerns on this invention is not restricted to the said manufacture example, You may combine the extract of the plant each containing inulin, quinic acid, caffeic acid, or its derivative (s). For example, instead of the burdock, instead of the burdock described in Production Examples 1 to 3, an extraction operation may be performed using chrysanthemums, coffee seeds, artichokes, apple fruits, and the like, and these extracts may be used in combination.

Test Example 1 Evaluation of type XVII collagen production promotion Normal human epidermal cells (NHEK) were seeded at 8 × 10 ³ cells / hole in a 96-well microplate supplemented with Humedia-KG2 medium [manufactured by Kurabo Industries, Inc.], 37 ° C., 5.0% CO After pre-culturing for 1 day under the conditions of ₂ , the Humedeia-KG2 medium prepared so as to contain the sample solutions 1 to 7 shown in Table 1 at a predetermined concentration was added, and further cultured under the same conditions for 2 days. Here, the density | concentration of the extract which concerns on the sample solutions 6 and 7 was adjusted so that final concentration might become 0.5% and 1.0% as a solution with respect to the quantity of a culture medium. Thereafter, immunodetection using a type XVII collagen antibody was performed. That is, after washing with PBS (-), cells were fixed by treatment with 15% neutral buffered formalin solution for 30 minutes, then permeabilized with 0.5% Triton X-100 solution for 1 hour, and 5-fold diluted blocking one. After blocking by treatment with P (Nacalai Tesque) solution for 2 hours, type XVII collagen antibody was added and allowed to stand at room temperature for 2 hours. Thereafter, the plate was washed with PBS (−), a fluorescently labeled secondary antibody was added, and the mixture was further allowed to stand in the dark for a certain time. After washing with PBS (-), fluorescence intensity was measured. First, the fluorescent label (Alexa Fluor594) of the secondary antibody was measured at Ex = 544 nm and Em = 590 nm (fluorescence microplate reader [Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)], and then DNA staining with Hoechst33342 was performed. Measurements were performed at Ex = 355 nm and Em = 460 nm. The degree of XVII collagen production was determined by dividing the fluorescence intensity of Alexa Fluor594 in each test group by the fluorescence intensity of Hoechst33342. In addition, the same procedure as above was performed for the control group without the sample solution (control) to which 50% 1,3-butylene glycol solution (50% BG solution) or PBS or DMSO was added instead of the sample solution. A relative value of the degree of type XVII collagen formation at the time of each sample addition to the degree of type XVII collagen production obtained in the above was determined and used as the XVII type collagen production rate (%).

[Table 1]
Table 1 shows sample solutions used in the evaluation test of the present invention.

The results of Test Example 1 are shown in Table 2.
[Table 2]

  As shown in Table 2, it was confirmed that the active ingredients (sample solutions 1 to 7) according to the present invention have an XVII collagen production promoting effect in epidermal cells. Since type XVII collagen retains hair follicle stem cells that play an important role in the initiation of the hair cycle in the bulge region, according to the present invention, the production of the collagen promotes the hair cycle to be healthy. It is possible to prevent and improve thinning and hair loss while maintaining a stable state.

Test Example 2 Evaluation of XVII collagen gene expression promoting effect Normal human epidermal cells (NHEK) were seeded at 6 × 10 ⁵ cells / hole in a 6-well plate supplemented with Humedia-KG2 medium [manufactured by Kurabo Industries, Ltd.], 5.0% CO _2, Culturing was performed at 37 ° C. under saturated steam. After culturing for 24 hours, sample solutions 1 to 7 shown in Table 1 were added and cultured. Here, the density | concentration of the extract which concerns on the sample solutions 6 and 7 was adjusted so that the final density | concentration as a solution with respect to the culture medium amount might be 0.5% and 1.0%. Further, as a control for comparison, a control group to which a 50% BG solution (1.0%) was added instead of the sample solution was set. After culturing for 24 hours, the cells in each test group and control group were collected with 1 mL of Trizol reagent (Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, mixed by stirring, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes using a centrifuge (TOMY / MX-160). Thereafter, 400 μL of the aqueous layer alone was collected. To the recovered aqueous layer, 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, mixed with stirring, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to totalRNA, stirred and washed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes to collect a precipitate. The recovered total RNA was subjected to a reverse transcription reaction using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) [manufactured by Takara Bio Inc.]) to synthesize cDNA. Using the synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.), and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.] Expression of type XVII collagen gene and expression of internal standard β-actin gene were detected. Here, β-actin is one of the housekeeping genes (a gene that is expressed in a certain amount in common in many tissues and cells and is always expressed and essential for cell maintenance and proliferation). Since the expression level is always constant, it is used as an internal standard in PCR experiments. As a test result, the expression level of each gene in each test section was compared when the expression level of β-actin gene was constant. In this test system, the relative value of the expression level of the XVII collagen gene in the other test plots when the expression level of the XVII collagen gene in the control plot was set to 100 was determined.

The results of Test Example 2 are shown in Table 3.
[Table 3]

  As shown in Table 3, it was confirmed that the active ingredients (sample solutions 1 to 7) according to the present invention have an effect of enhancing the expression of the type XVII collagen gene in epidermal cells.

Test Example 3 Evaluation of collagen XVII collagen gene expression promoting effect in hair follicular sheath cells 3 × 10 ⁵ cells / hole in human hair follicle sheath cells (HHORSC) added to MSCM medium [ScienCell Research Laboratories] The seeds were seeded and cultured at 37 ° C. under 5.0% CO ₂ and saturated steam. After culturing for 24 hours, sample solutions 5 and 7 shown in Table 1 were added and cultured. Here, the density | concentration of the extract which concerns on the sample solution 7 was adjusted so that the final concentration as a solution with respect to the amount of culture media might be 1.0%. Further, as a comparative control, a control group to which a 50% BG solution (1.0%) was added instead of the sample solution was set. After culturing for 24 hours, the cells in each test group and control group were collected with 1 mL of Trizol reagent (Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, mixed by stirring, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes using a centrifuge (TOMY / MX-160). Thereafter, 400 μL of the aqueous layer alone was collected. To the recovered aqueous layer, 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, mixed with stirring, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to totalRNA, stirred and washed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes to collect a precipitate. The recovered total RNA was subjected to reverse transcription reaction using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) [manufactured by Takara Bio Inc.]) to synthesize cDNA. Using the synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) (manufactured by Takara Bio Inc.) Expression of type XVII collagen gene and expression of internal standard β-actin gene were detected. As a test result, the expression level of the type XVII collagen gene in each test section was compared when the expression level of the β-actin gene was constant. In this test system, the relative value of the expression level of the type XVII collagen gene in the other test plots when the expression level of the type XVII collagen gene in the control plot was set to 100 was determined.

The results of Test Example 3 are shown in Table 4.
[Table 4]

  As shown in Table 4, it was confirmed that the active ingredients (sample solutions 5 and 7) according to the present invention have the effect of enhancing the expression of the type XVII collagen gene in the hair follicle root sheath cells.

Test Example 4 Evaluation of hair follicle stem cell growth promoting effect Remove hair follicle dermal papilla cells (Dermal sheath) from the scalp, trypsinize, stop reaction with trypsin inhibitor, transfer to collagen-coated culture dish, Cultured using Humedia-KG2. One day after the culture, a Humedeia-KG2 medium containing the sample solutions 5 and 7 shown in Table 1 was added, and further cultured under the same conditions for 3 days. Here, the density | concentration of the extract which concerns on the sample solution 7 was adjusted so that the final concentration as a solution with respect to the amount of culture media might be 1.0%. After culturing for 3 days, immunodetection was performed using an antibody of “CD34 protein” which is a hair follicle stem cell marker protein. That is, after washing with PBS (-), the cultured cells were fixed for 30 minutes using a 15% neutral buffered formalin solution, then permeabilized with 0.5% Triton X-100 solution for 1 hour, and 5-fold diluted blocking. After blocking with a one-P (Nacalai Tesque) solution for 2 hours, the CD34 antibody was added and allowed to stand overnight in a cool and warm dark room. After standing overnight, the plate was washed with PBS (−), a fluorescently labeled secondary antibody was added, and the mixture was further left standing in the dark for a certain time. After standing for a certain period of time, it was washed with PBS (−), and fluorescence observation was performed. The brightness value of the image obtained by fluorescence observation was measured using CS Analyzer ver 3.0 (ATTO). For the control group with no sample (control) added with 50% BG solution instead of the sample solution, the same operation as described above was performed, and the relative value of the luminance value at the time of each sample addition with respect to the luminance value of the control group was determined. CD34 production rate (%).

The results of Test Example 4 are shown in Table 5.
[Table 5]

  As shown in Table 5, it was suggested that the active ingredients (sample solutions 5 and 7) according to the present invention promote the proliferation of hair follicle stem cells.

Test Example 5. Evaluation test for normalization of hair cycle 20 subjects were divided into groups of 10 people, and each subject's head 1cm x 1cm hair was cut, and each was used as a test site for a microscope (Dino-Lite: Sanko Corporation) I took a photo at Three days later, photographs were taken for each test site, and the number of resting hairs was confirmed by image analysis. Thereafter, the sample solutions 5 and 7 shown in Table 1 were applied twice a day for 3 months to one of the test sites on the temporal region, and the “PBS (−) solution” as a control solution was similarly applied to the other. . Three months later, a photograph of the test site was taken with a microscope, and the resting hair before and after application of the sample solution was measured.

The test results were evaluated as follows.
(1) ++: The number of resting hairs decreased.
(2) +: There was no change in the number of hairs in the rest period.
(3)-: The number of resting hairs increased.

The results of Test Example 5 are shown in Table 6.
[Table 6]

  As shown in Table 6, application of the active ingredients (sample solutions 5 and 7) according to the present invention reduced the number of resting hairs in the subjects. Thereby, it was confirmed that this invention normalizes a hair cycle.

Test Example 6. Monitor Test Regarding Hair Growth / Hair Nourishing Effect A monitor test was conducted using the hair growth materials of Formulation Examples 1 to 8 and Comparative Example 1 shown in Tables 7 and 8. Divide subjects (30-65-year-old men) who are alopecia patients into groups of 5 people, and each hair growth material of Prescription Examples 1-8 and Comparative Example 1 is applied to the head twice a day. After application for 3 consecutive months, the increase in hair was evaluated based on the following criteria. The degree of increase in hair was evaluated using a photograph of the state of the scalp and hair.

[Table 7]

[Table 8]

[Evaluation criteria]
++: Increased hair was confirmed compared to before the start of the test.
+: No change was confirmed compared to before the start of the test.
-: A decrease in hair was confirmed before the start of the test.

The results of Test Example 6 are shown in Table 9.
[Table 9]

  As shown in Table 9, according to the present invention, it is possible to provide a hair-growth material having an excellent hair-growth / hair-growth effect.

Prescription examples other than the prescription examples shown in Table 1 are also shown below.
Formulation Example 9 Hair restorer [ingredient] part l-menthol 0.8
Adenosine 1.0
Extract of Production Example 1 2.0
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Ethanol 20.0
The amount in which the total amount of purified water is 100 parts The above ingredients were sufficiently stirred and mixed to obtain a hair restorer.

Formulation Example 10 Hair growth material A hair growth material was obtained in the same manner as in Formulation Example 9 except that minoxidil was used instead of adenosine in the ingredients of Formulation Example 9.

Formulation Example 11 Hair growth material A hair growth material was obtained in the same manner as in Formulation Example 9 except that 6-benzylaminopurine was used instead of adenosine in the components of Formulation Example 9.

Formulation Example 12. Hair restorer [ingredient] Part Potassium glycyrrhizinate
l-Menthol 0.1
Extract of Production Example 1 1.0
Soy lecithin 0.2
1,3-butylene glycol 10.0
Ethanol 20.0
Bamboo shoot extract 3.0
Brown algae extract 3.0
Hamcho Extract 3.0
Gentian extract 3.0
Amamo extract 3.0
Amount of purified water totaling 100 parts

Formulation Example 13 Hair-growth In Formulation Example 10, hair-growth was obtained in the same manner as in Formulation Example 10 except that the kuzu-root extract was used instead of the bamboo shoot extract and the powdery bark extract was used instead of the gentian extract.

Formulation Example 14. Hair restorer In Formulation Example 10, a hair restorer was obtained in the same manner as in Formulation Example 10, except that a hydrolyzed black soybean extract was used instead of the eel extract and a soymilk fermented liquor was used instead of the extract of Hamcho.

Formulation Example 15. Hair cream [ingredient] part liquid paraffin 15.0
Vaseline 15.0
Sara honey bee 2.0
Extract of Production Example 1 1.0
Brown algae extract 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Glycerin 5.0
1,3-butylene glycol 2.0
Polyoxyethylene hydrogenated castor oil 3.0
Chelating agent 0.1
Preservative 0.1
Fragrance 0.1
Dye 0.01
Amount of purified water totaling 100 parts

Formulation Example 16. Hair shampoo [ingredient] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Extract of Production Example 1 2.0
Collagen 5.0
Amamo extract 5.0
Black soybean hydrolyzate 5.0
Hamcho Extract 5.0
Paudalco Bark Extract 5.0
Kakkon Extract 5.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Formulation Example 17. Hair Shampoo A hair shampoo was obtained in the same manner as in Formulation Example 16 except that the extract of Production Example 2 was used instead of the extract of Production Example 1 in the ingredients of Formulation Example 16.

Formulation Example 18. Hair Shampoo A hair shampoo was obtained in the same manner as in Formulation Example 16 except that the extract of Production Example 3 was used instead of the extract of Production Example 1 in the ingredients of Formulation Example 16.

Example 19. Hair rinse [component A] part polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
Amount of purified water totaling 100 parts

Claims (4)

  A hair cosmetic comprising inulin, a plant containing the same or an extract thereof as an active ingredient.   A hair cosmetic comprising inulin and quinic acid or caffeic acid or a derivative thereof as active ingredients.   A hair cosmetic comprising, as an active ingredient, a plant containing inulin and quinic acid or caffeic acid or a derivative thereof, or an extract thereof.   A type XVII collagen production promoter comprising, as an active ingredient, a plant containing at least one of inulin, quinic acid or caffeic acid or a derivative thereof, or inulin, quinic acid, caffeic acid and a derivative thereof, or an extract thereof.