JP5947072B2 - Antioxidant - Google Patents

Description

  The present invention relates to an antioxidant containing a natural product as an active ingredient, and more particularly to an antioxidant having a free radical scavenging ability, high safety, and blended and used in foods and beverages and external preparations. is there.

In recent years, active oxygen has attracted attention as a factor that particularly oxidizes biological components, and its adverse effect on living organisms has become a problem. Active oxygen is generated in the process of energy metabolism in living cells. Examples of active oxygen include superoxide (that is, superoxide anion generated by one-electron reduction of oxygen molecules: .O ₂ ⁻ ), hydrogen peroxide (H ₂ ). O ₂ ), hydroxy radical (.OH), singlet oxygen ( ¹ O ₂ ) and the like. These active oxygens are essential for the phagocytic sterilization mechanism and play an important role in removing viruses and cancer cells.

  However, excessive production of active oxygen attacks in vivo molecules constituting membranes and tissues in the living body and induces various diseases. Normally, superoxide produced in vivo and used as a starting material for other active oxygen is sequentially eliminated by the catalytic action of superoxide dismutase (SOD) contained in the cells. Is excessive or when the SOD action is reduced, superoxide elimination is insufficient, and the superoxide concentration increases, which may cause tissue damage such as rheumatoid arthritis and Behcet's disease, myocardial infarction, stroke Cause cataracts, wrinkles, diabetes, arteriosclerosis, stiff shoulders, coldness.

  In particular, the skin is directly affected by external stimuli such as oxygen and ultraviolet rays, and is a tissue that is greatly damaged by oxidative stress due to active oxygen. In recent years, various active oxygens generated by ultraviolet rays cause sebum and lipid peroxidation, protein denaturation, enzyme inhibition, etc., which in the short term induce skin inflammation and the like. In the long term, it is thought to cause aging and cancer. Furthermore, active oxygen and lipid peroxide are considered to be involved in skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. Thus, active oxygen (free radical) is deeply involved in skin aging and skin diseases.

  Therefore, by inhibiting and suppressing the generation of active oxygen and in vivo radicals, skin aging such as wrinkle formation and reduced elasticity, tissue disorders such as rheumatoid arthritis and Behcet's disease, myocardial infarction, stroke, cataracts, diabetes, arteries It is considered that various disorders involving active oxygen such as hardening, stiff shoulders, and coldness can be prevented, treated or improved.

  In order to prevent oxidation due to active oxygen as described above, various antioxidants have been conventionally developed. For example, vitamin E and vitamin C known as antioxidants are free radical scavenging antioxidants in vivo. In addition, synthetic antioxidants such as BHT and BHA are also known. In addition, as plant-derived antioxidants, extracts of shiitake, enokitake, shimeji, kawatake, matsutake, garlic mushroom, spinach, nameko, and other basidiomycetes have been reported (Patent Documents 1 to 3). In addition, an antioxidant comprising an extract of the genus Pleurotus genus (Patent Document 4), an antioxidant comprising an extract of the genus Oleaceae (Patent Document 5), a pine bark extract and sesamin An antioxidant (Patent Document 6), an antioxidant composed of an extract of Sagaribana, Nanyouzakura, Quintra noo, etc. (Patent Document 7), an antioxidant composed of an extract of Rakan Fruit Flower (Patent Document 8), etc. Has been.

JP-A-5-317016 JP-A-6-65575 JP 59-124984 A JP-A-11-171723 Japanese Patent Laid-Open No. 11-171720 JP 2011-32334 A JP 2010-43012 A JP 2010-184873 A

  Thus, for plant-derived antioxidants, it is required to find new plants that exhibit new antioxidant power. Therefore, the present inventors have dealt with such a conventional situation and an object thereof is to provide a new plant-derived antioxidant.

  As a result of intensive studies in view of such a current situation, the present inventors have found that a specific plant extract has an excellent antioxidant action, and have completed the present invention.

  The present invention is (a) an antioxidant comprising as an active ingredient an extract of one or more plants selected from plants belonging to the genus Psophocarpus.

  Further, the present invention is (a) an extract of one or more plants selected from plants belonging to the genus Psophocarpus, and (b) an antioxidant containing royal jelly as an active ingredient.

  The antioxidant of the present invention has a free radical scavenging ability, has an excellent antioxidant action, and is a natural product-derived component, so it is highly safe and can be taken orally or transdermally.

It is a figure which shows the extent of the suppression suppression of the cell viability by a radical initiator when using a winged bean extract and a winged bean extract and royal jelly.

The plant belonging to the genus (a) leguminous winged bean (Psophocarpus) used in the present invention is a herbaceous tropical vine. In this invention, if it is a plant which belongs to the winged genus, it will not specifically limit, It can use arbitrarily. In the present invention, winged bean (scientific name: Psophocarpus tetragonolobus) is preferably used. “Urisun” is known as an improved variety. As a commercial product, the seeds can be purchased from Sakata Seeds.
The winged plant extract is conventionally known as a component having a laminin 5 production promoting action, a cell growth promoting action or the like (see Japanese Patent Application Laid-Open Nos. 2003-313135 and 2010-24211). The oxidation effect is not known and has been found for the first time by the present inventors.

  The extract of the plant belonging to the genus Psophocarpus used in the present invention is obtained by immersing or heating leaves, stems, branches, flowers, bark, seeds, pods, rhizomes or whole plants of the plant of the genus Psophocarpus together with an extraction solvent. After refluxing, it is obtained by filtration and concentration. Although any part of the plant can be used as the part to be used, it is particularly preferable to use seeds.

  An extract of a genus Psophocarpus can be obtained by a conventional method. For example, a genus Psophocarpus can be obtained by dipping or heating under reflux with an extraction solvent, followed by filtration and concentration. As the extraction solvent, any solvent that is usually used for extraction can be used. For example, water, methanol, ethanol, propylene glycol, 1,3-butylene glycol, glycerin and other alcohols, hydrous alcohols, chloroform , Organic solvents such as dichloroethane, carbon tetrachloride, acetone, ethyl acetate, hexane and the like can be used alone or in combination. The extract obtained by extraction with the above solvent is used as it is, or the concentrated extract is adsorbed by an adsorption method, for example, by removing impurities using an ion exchange resin, or by a porous polymer (for example, Amberlite XAD-2) column. Then, it can be used after elution with methanol or ethanol. Further, a partitioning method, for example, an extract extracted with water / ethyl acetate can be used.

  The extract thus obtained can be used as an antioxidant as it is or after further dilution with ethanol or the like, or after solidification, and the dried product can be used as it is, or the dried product can be redissolved in, for example, ethanol.

(Royal jelly)
Royal jelly is a special meal given to queen bees by bee workers. It is a milky white paste-like substance secreted from the hypopharyngeal gland and the maxillary gland of bees. The main ingredients are proteins, carbohydrates, fats, minerals, vitamins and so on.
As the royal jelly of the present invention, those collected by bees secreted for raising queen bees can be used, and those generally available on the market can also be used. As the royal jelly of the present invention, the royal jelly may be used as it is, or a dried product obtained by appropriately pretreating the royal jelly may be used.
The royal jelly used in the present invention is a milky white mucous substance secreted from a secretory gland in the head of a young bee worker. It is preferable to use one extracted with an extraction solvent.

(B) As for royal jelly, it has been known that the effect of preventing rough skin is obtained in combination with ginseng (JP-A-9-286709). It is also known that royal jelly-derived products have antioxidant activity (Japanese Patent Application Laid-Open No. 2005-263788, Japanese Patent Application Laid-Open No. 2009-274960, etc.).
However, according to experiments by the present inventors, royal jelly alone has no antioxidant activity, and it has been shown that it has excellent antioxidant activity only when a winged genus plant and royal jelly are used in combination.

  The antioxidant of the present invention comprises (a) an extract of one or more plants selected from plants belonging to the genus Psophocarpus, or the extract, and (b) royal jelly. Although it is preferable, other various components can be contained within a range not impairing the effects of the present invention.

  The antioxidant of the present invention contains (a) an extract of one or more plants selected from plants belonging to the genus Psophocarpus, and (b) a royal jelly. The oxidation effect is further enhanced.

  In the antioxidant of the present invention, when (a) a leguminous winged bean (Psophocarpus) plant extract and (b) royal jelly are included, the blending ratio is 1:10 to 80: 1 (in dry mass) (Mass ratio) is preferably combined. Within this range, the antioxidant effect is particularly excellent.

  The antioxidants described in the present invention can be used orally or percutaneously as they are or as various foods and cosmetics with various components added.

  As foods and beverages, the antioxidant described in the present invention is used as it is or various nutritional components are added, and the food is suitable, for example, powder, granule, granule, liquid, paste, cream, tablet , Capsule, caplet, soft capsule, tablet, rod, plate, block, pill, solid, gel, jelly, gummy, wafer, biscuit, bowl, chewable, syrup It can be formed into a shape or stick shape and provided as a food material. Further, it may be used by adding to water, milk, soy milk, fruit juice drink, whey drink, soft drink, green juice, yogurt and the like.

As cosmetics, for example, lotion, cosmetic cream, milky lotion, cream, pack, hair tonic, hair cream, shampoo, hair rinse, treatment, body shampoo,
It can be used as a cosmetic material in the form of facial cleansers, soaps, foundations, hair styling, etc.

  When the antioxidant of the present invention is used in a food, the amount (dry mass) of the antioxidant of the present invention can be appropriately determined according to the type, purpose, form, method of use and the like. For example, about 0.1-90 mass% can be made into food whole quantity. In particular, when used as a health food or drink, it is preferable to contain the active ingredient of the present invention in such an amount that a predetermined effect is sufficiently exhibited.

  In addition, the antioxidant of the present invention is 50 mg or more, preferably 50 mg, of an extract of one or more kinds of plants selected from among the plants belonging to the genus (a) leguminous family (Psophocarpus) in adults. It is preferred to administer ~ 1000 mg. In addition, (b) Royal jelly is preferably administered in an amount of 10 mg or more, preferably 50 mg to 2000 mg as raw royal jelly per day. If the dose per day is too small, the effect is not sufficiently exhibited. The upper limit of the dose per day is not particularly limited as long as the effect of the present invention is not impaired, but a remarkable effect commensurate with the increase in dosage cannot be obtained even if it is added in excess.

  As a form of transdermal absorption, for example, a skin cosmetic can be used. There is no restriction | limiting in particular and it can select suitably from various uses, For example, ointment, cream, emulsion, lotion, pack, jelly, lip balm, lipstick, bath agent, astringent, etc. are mentioned.

  The blending amount of the antioxidant according to the present invention with respect to the entire skin cosmetic can be adjusted as appropriate depending on the type of skin cosmetic, the physiological activity of the extract, etc., but the dry residue is 0.0001 to 10% by mass. Preferably, 0.001-1 mass% is more preferable.

If necessary, various main ingredients and auxiliaries usually used in the production of the skin cosmetics and other components can be used as long as the effects of the present invention are not impaired.
The other components are not particularly limited as long as they do not hinder the antioxidant action, and include components appropriately selected according to the purpose. Examples include astringents, bactericides, antibacterial agents, ultraviolet absorbers, cell activators. , Oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, and the like. These components, when used in combination with the weight loss agent, may act synergistically to provide superior effects than would normally be expected.

  The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto. Unless otherwise specified, the amount is shown in mass%.

Test Examples 1-4
A. Preparation of sample (1) Preparation of winged bean extract 50 wings of winged bean seeds were immersed in 90% ethanol for 1 week at room temperature, and the extract was concentrated to obtain 2.9 g of winged bean 90% ethanol extract (dried). It was.
The obtained winged bean extract is dissolved in dimethyl sulfoxide (DMSO) ml, and using this solution, the final concentration of the actual component is 0.005% (when used in combination with royal jelly solution), 0.01% (used in combination with royal jelly solution) If not, it was added to the medium.

(2) Preparation of Royal Jelly Solution 500 mL of 85% ethanol solution was added to 50 g of royal jelly, stirred for 10 minutes, and subjected to ultrasonic treatment for 1 hour. The insoluble component was filtered through an ultrafiltration membrane to obtain a royal jelly solution (raw royal jelly equivalent 20%, dry residue 7%).
The obtained solution was dissolved in a medium and added so as to have a final concentration of 0.005% (when used together with winged bean extract) and 0.01% (when not used together with winged bean extract).

B. Measurement of Antioxidant Activity (Free Radical Scavenging Activity) Human epidermal keratinocytes were seeded in a 24-well plate at a concentration of 1 × 10 ⁵ cells / ml in an epidermis keratinocyte-dedicated medium (KGM). After the cell adhesion, the basal medium (KBM) containing 0.1% BSA was replaced with a drug (test sample) added and left overnight.
The next day, the basal medium (KBM) containing 0.1% BSA supplemented with 80 mM of water-soluble radical initiator AAPH (2,2'-Azobis- (2-Amidinopropane) dihydrochloride) was replaced. The survival rate was compared with the value when 100 was not added the radical initiator.

C. Results The results are shown in Table 1 and FIG.

FIG. 1 is a graph of the results in Table 1.
As can be seen from the above results, when no drug was added, the cell viability decreased to 47.1% by the addition of the radical initiator (Test Example 1). When 0.01% winged bean extract was added, the decrease was significantly suppressed (Test Example 2). When both winged bean extract and royal jelly solution were added (0.005% each), the survival rate further increased (Test Example 3).
On the other hand, even when only 0.01% of the royal jelly solution was added in the presence of the radical initiator, the decrease in the survival rate due to the radical initiator could not be suppressed (Test Example 4).

  From these results, it was clarified that the addition of winged bean extract suppressed the decrease in the survival rate due to the radical initiator, and the effect was further enhanced by adding royal jelly.

Claims (2)

  (A) One or more plant extracts selected from plants belonging to the genus Psophocarpus and (b) an antioxidant containing royal jelly as an active ingredient. (A) The antioxidant according to claim 1, wherein the plant belonging to the genus Psophocarpus is a winged bean (scientific name: Psophocarpus tetragonolobus).

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