KR101506912B1 - Cosmetic composition for pore minimizing and antioxidant containing extracts of quince, lemon balm or orange blossom - Google Patents

Abstract

The present invention relates to a cosmetic composition for pore-shrinkage and antioxidation comprising at least one member selected from the group consisting of quinceia, lemon balm and orange blossom having sebum secretion inhibition, pore contraction, acne relief and anti-inflammatory effect.

Description

Technical Field [0001] The present invention relates to cosmetic compositions for pore minimization and antioxidant containing extracts of quinacridone, lemon balm or orange blossom,

The present invention relates to a cosmetic composition for pore-shrinking and antioxidation comprising, as an active ingredient, an extract comprising at least one selected from the group consisting of quince, lemon balm and orange blossom.

Skin is the primary protective membrane of the human body, which protects the internal organs of the body from changes in temperature and humidity, from external stimuli such as ultraviolet rays and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control. However, the cells constituting the skin are variously biochemically intracellularly stimulated by cell damage stimuli that damage cells such as excessive physical and chemical stimuli and stress applied to the skin from outside, nutritional deficiency, ultraviolet rays, environmental pollutants, Changes induce skin aging such as stain, freckles, dullness, loss of elasticity, keratinization, and wrinkles in skin appearance.

Depending on the condition of the skin surface, the skin is classified into several types such as dryness, neutrality, intelligence or complexity, which is determined by the amount of NMF (natural moisturizing factor) and sebum. When the amount of NMF and sebum is well balanced, the skin maintains its moist and smooth condition. On the other hand, if sebum is secreted too much, it is likely to become oily or acne skin. Because oily skin comes out of the pores of the skin, there is a lot of sebum secretion on the skin surface and pores are present, and the face looks dirty and makeup is easily erased. Therefore, overcoming these problems has been focused on the function of relieving skin discomfort caused by sebum secretion, refreshing skin and reducing skin irritation. The cosmetic material that performs this function is called " astringent agent ". The astringent is made by adjusting the skin condition close to the original shape and having a refreshing feeling, which is obtained by temporarily attracting the skin protein and suppressing excessive secretion of sebum or sweat. However, since chemical astringents cause many adverse effects on the human body, there are great limitations to their use. In order to overcome this problem, interest in natural astringents extracted from natural materials has started to be amplified and various research results have been published.

If sebum is secreted excessively, various diseases such as acne become worse, pore expansion due to acne is promoted, seborrheic dermatitis occurs, and so on. However, since the substances developed so far have been developed for the purpose of treating diseases such as acne rather than substances capable of eliminating the root cause thereof, their healing effects are insufficient and there is no preventive concept.

The present invention aims to provide a cosmetic composition which is excellent in suppression of sebum secretion, pore contraction, acne relief, antioxidant and anti-inflammatory effect, comprising at least one extract selected from the group consisting of quince, lemon balm and orange blossom.

To achieve the above object, the present invention provides a cosmetic composition for pore-shrinking and antioxidation comprising at least one extract selected from the group consisting of quince, lemon balm and orange blossom.

The cosmetic composition comprising at least one member selected from the group consisting of quinceons, lemon balm and orange blossom of the present invention exhibits sebum secretion inhibition, pore contraction, acne relief, antioxidant and anti-inflammatory effect.

Hereinafter, the present invention will be described in detail.

The present invention relates to a cosmetic composition for shrinking pores and antioxidation comprising at least one extract selected from the group consisting of quince, lemon balm and orange blossom, and has anti-sebum secretion, pore contraction, acne relief, antioxidant and anti-inflammatory effect.

The raw material of the extract contained in the cosmetic composition according to the present invention is a quince tree ( Chaenomeles sinensis ) is an oval or ball shaped. Promotes the secretion of digestive enzymes to improve the digestive function, making tea or soak to drink. At first it is green, but when it is ripe, it becomes yellowish and uneven. The fragrance is excellent, but the taste is tender and the skin is hard and it is hard to eat it. It has an essential oil ingredient on the surface and it is sticky, which adds flavor and efficacy. It is presumed that it originated in China before the Joseon Dynasty in Korea. Jeollanamdo, Chungcheongnam-do and Gyeonggi-do, and it is distributed in China and Japan. Alkaline foods contain sugars (fructose), calcium, potassium, iron, and vitamin C, tannin and tannin, and acidity such as malic acid and citric acid. It promotes the secretion of digestive enzymes and improves the digestive function, so when you are sick or diarrhea, you will feel comfortable. It is good for the metabolism to release the hangover, and to remove the phlegm, it is used as medicine for cold, bronchitis, pneumonia in one place. It is also effective in throat disease, but the amount of urine is reduced. When making a tea, slice it thinly 2mm thick, and then boil it with 1 side of ginger, boil it lightly, put it in honey or sugar, and put it in hot water. The liquor is sliced thinly, poured soju, and made with sugar. In addition, boiled well, soaked in honey, safflower, boiled and mashed, then put honey and water to cook and make the process of moo.

The ingredients of the extracts contained in the cosmetic composition according to the present invention, such as lemon balm (Lemon Balm,Melissa officinalis) Is a perennial plant of cold tolerance growing about 60 to 150 cm in height and its stem is square. Leaf length is about 8㎝ wide ovate. It has petiole and hair and strong lemon scent. Flowers are white, yellow and light blue in late summer, and petals are attached to each other. Flowers, leaves and stems can be used. Drinking the leachate after meals helps digestion and can be made into a herbal tea that suppresses kotatsu (symptoms of stomach upset) and colic. The leachate has a sweating effect and is effective in the treatment of colds and influenza. Leaves and essential oils are used in aromatherapy and are effective for anxiety, depression, insomnia and nervous headaches. Scientific reports that essential oils have anticonvulsant activity are the basis for this use. It also has an antihistamine action and is recommended for allergic patients who develop eczema. It is also a medicinal plant used for promoting menstruation and relieving menstrual pain. Polyphenols and tannins in these plants also have antiviral action. It has also been used as medicine for longevity. If you use the leaves as a bath agent, the lemon incense is similar to the direction, it feels light and comfortable, and the effect of keeping warm and skin is high. You can prevent hair loss by using a myrrh solution as a rinse. Raw leaves reduce pain when applied to insect bites.

The orange blossom, which is the raw material of the extract contained in the cosmetic composition according to the present invention, is orange flower. An orange is an evergreen broad-leaved shrub with a horned branch. The stem is about 7 m high, with thorns on the branches, and the leaves are oval-shaped. White flowers bloom in early summer and fruit is ripe in October with orange berries. The fruit is edible and used as a raw material for the foundation, the emergence medicine, the seasoning or the perfume. The major components are limonene, hesperidin, naringin, and leuproline.

In the present invention, it is preferable to clean and dry the corn, lemon balm and orange blossom with purified water.

In addition, the present invention provides a cosmetic composition comprising a quince, lemon balm and orange blossom, wherein the mixed composition ratio of the quince, lemon balm and orange blossom can be 1: 1: 1 to 3: 2: 2, Suppress sebum secretion, shrink pores, relieve acne and have anti-inflammatory effects.

In the cosmetic composition of the present invention, at least one extract selected from the group consisting of quince, lemon balm and orange blossom is contained in an amount of 0.001% to 30% by weight in powder form or liquid form relative to the total weight of the cosmetic composition, Is 0.01 to 30% by weight. Within the above range, sebum secretion suppression, pore contraction, acne relief, antioxidant and anti-inflammatory effects can be maximized.

The extraction solvent of the extract of the present invention may be water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol, butylene glycol and a mixed solution of two or more of the above solvents And preferably one selected from the group consisting of water, anhydrous or hydroalcohol having 1 to 4 carbon atoms, and a mixed solution of the solvent. The anhydrous or hydrated alcohol having 1 to 4 carbon atoms is methanol, ethanol, n-propanol, iso-propanol, n-butanol and tert-butanol. Among the above-mentioned extraction solvents, functional ethanol is most preferably used, and the amount of water contained is preferably 30 to 90% by weight, and most preferably 70% by weight.

One or more extracts selected from the group consisting of the quince, lemon balm and orange blossom of the present invention are prepared by known extraction methods known to the ordinarily skilled artisan. For example, the extraction solvent of one to fifty times the volume of one or more dry mass selected from the group consisting of quince, lemon balm and orange blossom of the present invention is preferably water, an anhydrous or hydrolyzate having a carbon number of 1 to 4 Alcohols are added and the mixture is immersed at 4 to 30 DEG C for 3 to 20 days to extract the active ingredient, and then the extraction solvent is obtained by concentration with a vacuum concentrator. Further, in order to prevent the solvent from evaporating, it is extracted with an extractor equipped with a cooling condenser at 30 to 100 ° C for 1 to 48 hours or at 5 to 30 ° C for 1 to 10 days to extract an active ingredient, And concentrated by a reduced pressure concentrator. In the above-mentioned heat extraction method, the extraction temperature is preferably 50 to 100 ° C. In addition to the above-described production methods, ultrasonic extraction may be used.

The extract of at least one member selected from the group consisting of quinceons, lemon balm and orange blossom of the present invention may be a concentrate concentrated by reduced pressure, a lyophilized concentrate or a powder dried by spray drying the concentrated concentrate.

The cosmetic composition of the present invention is not particularly limited in its formulation. For example, it may be formulated such as lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion type foundation, solid type foundation, Lt; / RTI > It may also be a formulation such as a bath cleanser, shampoo, soap, mist or aerosol.

In addition, in the cosmetic composition of each formulation, components other than the above-mentioned one or more extracts selected from the group consisting of quince, lemon balm and orange blossom can be arbitrarily selected and formulated according to the formulation or use purpose of other cosmetics . For example, it may include a coloring agent (coloring matter), a flavoring agent (fragrance), a suspending agent, an emulsifying agent, a solubilizing agent, a stabilizer, an isotonic agent, a pH adjusting agent, a viscosity adjusting agent,

The cosmetic composition of the present invention may further contain at least one active ingredient selected from the group consisting of quince, lemon balm and orange blossom in addition to one or more extracts exhibiting sebum secretion suppression, pore contraction, acne relief, antioxidant and anti-inflammatory effects .

Hereinafter, the present invention will be described in more detail by way of Examples, Experimental Examples and Formulation Examples. However, the following Examples, Experimental Examples and Formulation Examples exemplify the present invention, and the present invention is not limited to the following Examples, Experimental Examples and Formulation Examples.

Example  1-3, Lemon balm  And Orange blossom  Preparation of each extract powder

(Example 1), lemon balm (Example 2) and orange blossom (Example 3) were washed with purified water, and 1 kg of distilled water was added to 50 g of each sample. And extracted with boiling time. The above procedure was repeated three times. Thereafter, the mixture was filtered through a 300-mesh filter paper, left at room temperature for one week, and the precipitate was filtered twice through a filter paper of Edbentek No. 5 and a Wattman GFC 150 mm filter. Thereafter, the mixture was concentrated to a temperature of 45 캜 by using a vacuum concentrator, and 5 g of the extract powder of the title (Example 1) and 4 g of a dried extract (Model B-290, manufactured by BUCHI) Example 2) and 0.5 g (Example 3).

- Inlet temperature: 180 ℃

- Aspiratoe efficiency: 100% (35 cm3 / hr)

- Pump efficiency: 25% (7.5 ml / min)

- Nozzle Cleaner 4

- Rotameter: 30 mm (357 L / hr)

Example  4-10. Lemon balm  And Orange blossom  Mixed Preparation of Extracted Powder

Lemon balm and orange blossom extracted powders of Examples 1 to 3 were mixed in the ratio as shown in Table 1 below.

division Mixing ratio
(Quince, lemon balm and orange blossom) Example 4 1: 1: 1 Example 5 2: 1: 1 Example 6 1: 2: 1 Example 7 1: 1: 2 Example 8 2: 2: 1 Example 9 1: 2: 2 Example 10 2: 1: 2

The extracts prepared in Examples 1 to 10 were dissolved in purified water to prepare 0.1, 0.2, 0.5, 1, 2 and 5 wt%, respectively, and used in Experimental Examples 1 to 8 to confirm the effect.

Experimental Example  1. Confirmation of pore contraction effect

0.1, 0.2, 0.5 and 1 wt% of the extracts of Examples 1 to 10 were used to confirm the pore-shrinking effect.

Using albumin as a protein substitute for skin, we confirmed the effect of pore shrinkage.

1 ml of a sample was added to 2 ml of a solution of 0.3% albumin dissolved in a pH 4.5 citric acid buffer. After stirring for 5 minutes, the absorbance was measured at 650 nm. Purified water was used as a control. The higher the absorbance value, the better the pore shrinkage effect. The results are shown in Table 2 below.

division Absorbance 0.1% 0.2% 0.5% 1.0% Control group 0.004 0.004 0.004 0.004 Example 1 0.496 0.713 1.137 1.682 Example 2 0.413 0.648 1.007 1.635 Example 3 0.211 0.475 0.792 1.276 Example 4 0.475 0.692 1.088 1.719 Example 5 0.481 0.709 1.156 1.964 Example 6 0.459 0.784 1.226 2.013 Example 7 0.347 0.549 0.885 1.498 Example 8 0.518 0.831 1.394 2.137 Example 9 0.479 0.764 1.247 1.893 Example 10 0.418 0.691 1.183 1.712

From the above results, all of the extracts of Examples 1 to 10 showed excellent results, and the mixed extract of Example 8 showed the most excellent results.

Experimental Example  2. Confirmation of inhibition of sebum secretion

The amount of sebum secretion was measured in 10 oily skin owners between 20 and 50 years of age. On the left side of the forehead, 5% by weight of the extract of Example 8 and the right side of the forehead were treated with purified water twice a day in the morning and evening, and after one week, two weeks, three weeks and four weeks, The amount of sebum secretion in the skin was measured using an oil meter (Sebum meter SM 810). The experiment was carried out at 20 ° C and 20% relative humidity. In the case of oily skin, the measured value is 220 / / cm2 or more, and in the case of normal skin, it is 100 to 220 / / cm2.

The results are shown in Table 3 below.

Name of sample 0 days 7 days 14 days 21st 28th Control group 250 245 240 235 230 Example 8 250 235 225 210 200

As a result of Table 3, it was confirmed that Example 8 had an effect of suppressing sebum secretion.

Experimental Example  3. Identification of antimicrobial effect of acne

0.1, 0.2, 0.5 and 1% by weight of the extracts of Examples 1 to 10 were used to confirm the antimicrobial effect of acne.

The antimicrobial effect of Propionibacterium acnes, a cause of acne, was confirmed by the method of Diac Plate method. In this method, the strain was plated on an agar plate medium and the sample was placed on a paper disc and cultured, Inhibition zone) was measured and the antimicrobial activity was measured.

The results are shown in Table 4 below.

division Inhibition Zone (mm) / Concentration 0.1% 0.2% 0.5% 1.0% Example 1 One 2 2 4 Example 2 2 3 4 5 Example 3 0 0 One 2 Example 4 One 2 2 2 Example 5 One 2 3 4 Example 6 One One 3 5 Example 7 0 One One 3 Example 8 2 4 8 15 Example 9 One 3 5 5 Example 10 2 3 4 5

Table 4 shows excellent results. Among them, Example 8 showed excellent antimicrobial effect against acne.

Experimental Example  4. Nitrite ( NO ) Confirmation of scavenging activity effect

The effect of nitrite scavenging activity was confirmed using 0.1, 0.2, 0.5, 1, 2 and 5 wt% extracts of Examples 1 to 10.

Nitrite (NaNO ₂ ) scavenging activity was measured using the method of Kato et al. (Kato H. et al., Agric. Biol. Chem. 51, p1333, 1987). 1 ml of the above extract was added to 2 ml of 1 mM sodium nitrite (NaNO ₂ ) solution, and the pH of the reaction solution was adjusted to pH 1 using a 0.1 N hydrochloric acid solution (HCl) and a 0.2 M citrate buffer 1.2, 3.0, and 6.0, and the volume of the reaction solution was adjusted to 10 ml. The reaction solution was reacted at 37 ° C for 1 hour. One ml each of the reaction solution was added thereto, and 5 ml of 2% acetic acid was added. Then, 0.4 ml of a grease reagent (Griess Reagent, A: B = 1: 1, A: a 1% solution of sulfanilic acid in 30% acetic acid, and B: a 1% naphthylamine solution in 30% acetic acid) The reaction was allowed to proceed at room temperature for 15 minutes. Purified water was used as a control, and the absorbance at 520 nm was measured to confirm the nitrite scavenging activity.

The nitrite scavenging activity was calculated by the following equation (1), and the results are shown in Table 5 below.

division Nitrite scavenging activity (%) 0.1% 0.2% 0.5% 1.0% 2.0% 5.0% Control group 0 0 0 0 0 0 Example 1 10 12 20 28 37 52 Example 2 12 13 20 33 45 67 Example 3 9 21 27 57 72 85 Example 4 19 31 47 66 80 91 Example 5 11 22 38 50 71 79 Example 6 8 12 17 24 33 50 Example 7 10 19 36 48 61 73 Example 8 21 33 50 68 84 95 Example 9 10 20 37 51 70 81 Example 10 9 12 21 28 40 61

From the results of Table 5, all of Examples 1 to 10 exhibited nitrite scavenging activity, and the nitrite scavenging effect of Example 8 was superior to those of the other Examples.

Experimental Example  5. NO Confirming the production inhibitory effect

In order to confirm the anti-inflammatory activity effect, the effect on inhibition of NO (nitric oxide) production, which is known to cause inflammation induction, was examined through the extracts of 0.5, 1, 2 and 5% by weight in Examples 1 to 10. Although normal NO formation plays an important role in killing bacteria or eliminating tumors, NO produced by iNOS in the inflammatory state not only promotes inflammatory responses such as vascular permeability and edema, but also stimulates inflammation mediator biosynthesis, It is known to intensify.

Raw 264.7 cells were adjusted to 5 × 10 5 cells / well using DMEM medium supplemented with 10% FBS (Fetal Bovine Serum), and inoculated on a 24-well plate. After the samples were treated for 30 min, LPS (Lipopolysaccharide, / Ml) was added and cultured for 24 hours. The amount of NO produced was calculated by using the NO detection kit (iNtRON) using the following equation (2). As a control group, an experimental group treated with LPS alone was used.

The NO production inhibition rate was calculated by the following formula 2, and the results are shown in Table 6 below.

division NO production inhibition rate (%) 0.5% 1.0% 2.0% 5.0% Control group 0 0 0 0 Example 1 4 10 21 46 Example 2 7 16 29 54 Example 3 10 19 40 66 Example 4 14 31 56 73 Example 5 8 15 23 51 Example 6 11 20 29 44 Example 7 18 33 45 60 Example 8 28 48 70 85 Example 9 21 35 56 77 Example 10 12 25 39 51

As a result of Table 6, all of Examples 1 to 10 showed inhibitory effect on NO production, and in particular, Example 8 showed superior NO production inhibitory effect to those of other Examples.

Experimental Example  6. Confirmation of inhibition of prostaglandin formation

1, 2 and 5% by weight of the extracts of Examples 1 to 10 were used to inhibit the production of prostaglandin E ₂ (prostaglandin E ₂ , PGE ₂ ), which is known to cause inflammation induction. Cyclooxygenase (COX) is an enzyme that converts arachidonic acid into prostaglandin, classified as COX-1 and COX-2. COX-1 acts on normal biological functions such as platelet formation, gastric wall protection and maintenance of renal function in the body, and COX-2 forms PGE ₂ , an inflammation mediator. PGE ₂ is known to be deeply involved in the development of cancer, such as promoting inflammation, immune response, and angiogenesis.

Raw 264.7 cells were adjusted to 5 × 10 5 cells / well using DMEM medium supplemented with 10% FBS (Fetal Bovine Serum), and inoculated on a 24-well plate. After the samples were treated for 30 min, LPS (Lipopolysaccharide, / Ml) was added and cultured for 24 hours. The amount of produced PGE ₂ PGE ₂ The values measured using an ELISA kit (R & D Systems, Inc, USA) were calculated by the following equation (3). As a control group, an experimental group treated with LPS alone was used.

The inhibition rate of PGE ₂ production was calculated by the following equation (3), and the results are shown in Table 7 below.

division PGE2 production inhibition rate (%) 0.5% 1.0% 2.0% 5.0% Control group 0 0 0 0 Example 1 9 12 19 43 Example 2 14 25 30 47 Example 3 21 34 48 61 Example 4 31 39 61 74 Example 5 12 14 31 49 Example 6 20 31 40 49 Example 7 24 37 48 61 Example 8 35 51 66 82 Example 9 33 46 58 70 Example 10 22 30 39 51

As shown in Table 7, all of Examples 1 to 10 showed excellent results. It was confirmed that Example 8 was superior to other Examples in inhibiting PGE ₂ formation.

Experimental Example  7. TNF -α production inhibitory effect

In order to confirm the anti-inflammatory effect, the inhibitory effect of TNF-α, which is one of the early inflammatory molecules, was confirmed using the extracts of 0.5, 1, 2 and 5% by weight in Examples 1 to 10. The formation of pro-inflammatory cytokines, such as TNF-α, is mediated through the process of converting arachidonic acid into prostaglandin due to the activity of phospholipase A2 (A2) .

Raw 264.7 cells were adjusted to 5 × 10 5 cells / well using DMEM medium supplemented with 10% FBS (Fetal Bovine Serum), and inoculated on a 24-well plate. After the samples were treated for 30 min, LPS (Lipopolysaccharide, / Ml) was added and cultured for 24 hours. The amount of TNF-α produced was calculated using the TNF-α ELISA kit (eBioscience, Inc, USA) by the following equation (4). As a control group, an experimental group treated with LPS alone was used.

The inhibition rate of TNF-? Production was calculated by the following equation (4), and the results are shown in Table 8 below.

division TNF-α production inhibition rate (%) 0.5% 1.0% 2.0% 5.0% Control group 0 0 0 0 Example 1 9 11 24 39 Example 2 11 20 27 51 Example 3 19 34 50 64 Example 4 20 33 59 76 Example 5 13 21 36 50 Example 6 28 41 56 66 Example 7 30 44 58 70 Example 8 45 61 73 88 Example 9 41 57 68 80 Example 10 10 19 31 48

As shown in Table 8, the anti-inflammatory activity of Examples 1 to 10 was excellent, and the anti-inflammatory activity of Example 8 was superior to that of the other Examples.

Experimental Example 8 . Confirmation of inhibition of inflammation caused by ultraviolet light

Using 0.5, 1, 2 and 5% by weight of the extracts of Examples 1 to 10, the inhibition of inflammation caused by ultraviolet light was confirmed.

Human keratinocytes were inoculated into DMEM medium containing penicillin (100 IU / ml), streptomycin (100 g / ml) and 10% FBS (Fetal Bovine Serum) ≪ / RTI > The obtained keratinocytes were divided into 3 × 10 5 cells per well in a 6-well plate and cultured for 24 hours. After incubation, the cells were irradiated with 25 mJ / cm 2 of ultraviolet light, added with the sample, and further cultured for 24 hours. The amount of TNF-α and IL-1α produced was calculated using an ELISA kit (eBioscience, Inc, USA) using the above Equation 4 and Equation 5. As a control group, an experimental group treated with ultraviolet light alone was used.

TNF-a and production inhibition rate were calculated by the above-described Equation (4) and Equation (5), and the results are shown in Tables 9 and 10 below.

division TNF-α production inhibition rate (%) 0.5% 1.0% 2.0% 5.0% Control group 0 0 0 0 Example 1 5 10 18 31 Example 2 6 11 23 46 Example 3 10 21 30 51 Example 4 22 29 44 59 Example 5 10 15 25 47 Example 6 7 12 21 33 Example 7 8 19 30 44 Example 8 27 36 49 68 Example 9 21 30 41 55 Example 10 6 11 19 30

As shown in Table 9, all of Examples 1 to 10 showed excellent results. In particular, Example 8 showed better inhibitory effect on TNF-? Production than the other Examples.

division Inhibition rate of IL-1-α production (%) 0.5% 1.0% 2.0% 5.0% Control group 0 0 0 0 Example 1 7 20 31 42 Example 2 20 28 35 50 Example 3 18 29 48 61 Example 4 23 36 54 67 Example 5 19 26 41 53 Example 6 22 31 39 50 Example 7 20 32 44 58 Example 8 48 60 73 89 Example 9 41 52 66 75 Example 10 20 30 41 51

As shown in Table 10, all of Examples 1 to 10 showed excellent results, and in particular, Example 8 showed better inhibitory effect on IL-1? Production than the other Examples.

Formulation Example  One. Example  Preparation of lotion containing mixed extract of 8

1 kg of lotion (skin lotion) containing the mixed extract powder of Example 8 was prepared using the following production method in the contents shown in Table 11 below.

number Raw material Content (% by weight) One The mixed extract of Example 8 1.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene hardened castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 antiseptic a very small amount 9 Pigment a very small amount 10 Spices a very small amount 11 Purified water Balance

In Table 11, substances No. 2, No. 3, No. 4 and No. 8 were sequentially injected into the reaction vessel and dissolved by stirring. Subsequently, the No. 5 substance was dissolved by heating at about 60 ° C. Thereafter, the substance 10 was dissolved and added to the substance 11. Finally, 1, 6, 7, and 9 substances were added, stirred well, and aged at 25 ℃ for 3 days to prepare the title lotion.

Formulation Example  2. Example  Preparation of nutrition lotion containing mixed extract of 8

1 mg of hydrous nutritive lotion containing the mixed extract powder of Example 8 was prepared in the following Table 12 using the following manufacturing method.

number Raw material Content (% by weight) One The mixed extract of Example 8 1.0 2 Wax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbitan stearate 1.0 7 Pro-type glycerin monostearate 0.5 8 Stearic acid 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Balance

In Table 12, the materials 10, 11, 13, and 16 were heated to 80 to 85 ° C while mixing and stirring. 9 and 12 were heated and dissolved in the temperature range of 80 to 85 ° C, and emulsified. After the emulsification is completed, the mixture is cooled to 50 ° C with stirring using an agitator, and the substance 15 is added. After cooling to 45 ° C, the substance 14 is added, and the substance 1 is added at 35 ° C. For 3 days to prepare the nutritional lotion of the title.

Formulation Example  3. Example  Nutrient containing mixed extract of 8 Serum  Produce

1 kg of a hydrosomal nutrition serum containing the mixed extract powder of Example 8 was prepared in the following Table 13 with the following preparation method.

number Raw material Content (% by weight) One The mixed extract of Example 8 1.0 2 Xanthan gum 0.02 3 Butylene glycol 10.0 4 Phospholipid 10.0 5 Hydroxyethyl cellulose 0.1 6 Sodium hyaluronate 5.0 7 Purified water Balance

In Table 13, materials 2, 3, and 5 were heated to a temperature ranging from 40 to 45 ° C. while being mixed and stirred. Then, the mixture was injected into a reactor, and then an emulsifier was activated. Materials 4, 6 and 7 were injected into the reactor and emulsified. After completion of the emulsification, the mixture was cooled to 35 ° C with stirring using a stirrer, and the material 1 was added thereto, cooled to 25 ° C, and aged at 25 ° C for 3 days to prepare a nutritional serum.

Formulation Example  4. Example  Preparation of essence containing mixed extract of 8

1 kg of a hydrosomal essence containing the mixed extract powder of Example 8 was prepared in the following Table 14 using the following production method.

number Raw material Content (% by weight) One The mixed extract of Example 8 1.0 2 Sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 Ceramide 0.7 5 Steareth-4 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Xanthan gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water Balance

In Table 14, materials 2, 3, 4, 5 and 6 were homogenized at 65 ° C to prepare nonionic amphipathic lipids. The non-ionic amphiphilic lipids and materials 1, 7, 8, and 14 were mixed and homogenized at 65 ° C, passed through a microfluidizer, and then material 9 was gradually warmed to a temperature range of 50 to 60 ° C After homogenization, it was passed again through microfluidizer. Subsequently, substances 10, 11, 12 and 13 were added, dispersed, stabilized and aged to prepare the title essence.

Experimental Example 9 . Confirm formulation stability

The formulations prepared in Formulation Examples 1 to 4 including the mixed extract powder of Example 8 were stored in a room, a refrigerator and an incubator kept constant at room temperature (25 ° C), cold (4 ° C) and constant temperature (45 ° C) After storage for 12 weeks in an opaque glass container, discoloration, removal and phase separation were observed and stability was confirmed.

Temperature
Condition Stability verification (discoloration, removal and separation) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Indoor (25 ℃) 0 0 0 0 Refrigerated (4 ℃) 0 0 0 0 Constant temperature (45 ° C) 0 0 0 0

<Formulation stability level>

0: No change, 1: Minute change, 2: Change, 3: Extreme change

As shown in Table 15, neither of the formulations of Formulation Examples 1 to 4 exhibited discoloration, separation and separation phenomena under the temperature conditions of 25 캜, 4 캜 and 45 캜, and was stable.

Claims (7)

A cosmetic composition for pore shrinkage and antioxidation comprising a composition of 2: 2: 1 of an extract of corn, lemon balm and orange blossom. delete delete The cosmetic composition for shrinking and antioxidation pores according to claim 1, wherein the extract is 0.001 to 30% by weight based on the total weight of the cosmetic composition. [Claim 2] The method according to claim 1, wherein the extraction solvent is selected from the group consisting of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol, butylene glycol, Wherein the cosmetic composition is one kind selected from the group consisting of polyvinylpyrrolidone and polyvinylpyrrolidone. [Claim 6] The cosmetic composition for contraction and antioxidation of pores according to claim 5, wherein the extraction solvent of the extract is one selected from the group consisting of water, anhydrous or hydroalcohol having 1 to 4 carbon atoms, and a mixed solution of the solvent. The cosmetic composition according to claim 1, wherein the cosmetic composition is a formulation of lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion type foundation, solid type foundation, ultraviolet screening cream, bath detergent, shampoo, soap, mist or aerosol Wherein the cosmetic composition is a composition for shrinking pores and antioxidants.