KR20220028612A - Cosmetic composition antimicroboal, antioxidation, whitening or anti-inflammation comprising artemisia capillaris thunberg and olive extract - Google Patents

Abstract

The present invention relates to a cosmetic composition including an Artemisia campestris extract and an Olea europaea extract as active ingredients, which can prevent and alleviate skin damage by antibacterial, antioxidant, whitening and anti-inflammatory activities. Also, provided can be a cosmetic composition which can exhibit an excellent antiseptic effect and excellent effects in skin aging prevention and alleviation, skin wrinkle alleviation, whitening, and skin inflammation alleviation.

Description

A cosmetic composition for antibacterial, antioxidant, whitening, and anti-inflammatory containing extracts of Injin mugwort and olive as active ingredients

The present invention relates to a cosmetic composition for antibacterial, antioxidant, whitening, and anti-inflammatory comprising an extract of Injin mugwort and an olive extract as active ingredients. In more detail, a cosmetic composition with antibacterial, antioxidant, whitening and anti-inflammatory effects by including ginseng mugwort extract and olive extract as active ingredients, and excellent antiseptic effect, skin aging prevention and improvement, skin wrinkle improvement, whitening, and skin inflammation improvement is about

As the most external organ of the human body, the skin acts as an essential barrier to protect the body by protecting moisture and blocking intrusion factors from the outside.

Cosmetics generally aim to clean and beautify the skin, and in the process accompanying this purpose, it cleans and moisturizes the skin, blocks UV rays, and removes free radicals and peroxides from the body caused by pollution and stress to reduce skin aging. Gives the effect of delaying

Most of the raw materials constituting cosmetics are substances suitable for use by microorganisms. In addition, in recent years, the possibility of contamination of cosmetics by microorganisms has increased by adding a lot of natural products obtained from animals and plants in order to develop high-functional cosmetics.

Accordingly, preservatives are added to cosmetics, and preservatives used in cosmetics must show their effectiveness among consumer products, and whether an appropriate preservative system is established must be proven by testing.

When cosmetics are contaminated by microorganisms, the negative aspects they give us can be divided into two main categories. The first is the risk to consumer health, and the second is the decline in product value. When cosmetics contaminated with microorganisms are applied to the skin, microorganisms introduced through wounds or cracks on the skin may cause inflammation or various troubles. In addition, it may bring about physical changes (destruction of oil painting and odor, etc.) of cosmetics due to the growth of microorganisms, thereby reducing the value of the product. Therefore, it is necessary to identify various sources of contamination that may occur from prescription to manufacturing to packaging and to take countermeasures so that cosmetics are not contaminated with microorganisms.

Cosmetics consist of ingredients that are good for microorganisms, such as water, oil, moisturizer, surfactant, polymer, and natural product. Cosmetics have the characteristic of being used by consumers for a long period of time, so an appropriate preservative is required to prevent deterioration by various microorganisms. If a cosmetic contaminated with microorganisms is used, it may be harmful to the user and may cause disease in those with weak immunity. For this reason, cosmetics should have a preservative system at an appropriate level according to the characteristics of each formulation.

Skin aging can be broadly divided into natural aging and extrinsic aging. Extrinsic aging is mainly affected by sunlight, and in particular, ultraviolet rays are well known to cause photo-aging. On the other hand, infrared radiation is known to have a beneficial effect by increasing heat in the body.

One of the causes of skin aging is caused by active oxygen such as superoxide anion radical, hydroxy radical, singlet oxygen and hydrogen peroxide (H ₂ O ₂ ) derived from oxygen. It is known that the free radical reaction destroys major macromolecules of cells including lipids, which are highly reactive and cause abnormal metabolism by reacting with cell components or cell membranes. Such abnormal metabolism or exposure to physical and chemical external stimuli causes an imbalance between oxidation and antioxidant, which causes peroxidation or incapacity of the body's defense mechanisms, causes oxidation and denaturation of proteins, and impairs cell functions. Eventually, skin aging is accelerated, which is characterized by reduced elasticity, wrinkles, melasma and freckle production (J. Soc., Cosmet. Sci. Kor., 23, pp75-132, 1997).

In particular, one of the many causes of aging and diseases of living things living in the natural world can be found in active oxygen generated in the oxidative metabolic process. Although it is an essential material for non-specific removal of non-magnetic substances, it is known to cause peroxidation of lipids by participating in free radical reactions in biofilms mainly rich in unsaturated fatty acids.

Substances with active oxygen suppression and antioxidant effects that are used as raw materials for general cosmetics, pharmaceuticals and food are known, but their use is limited because they are synthetic products that do not ensure safety when applied to human skin for a long period of time.

Most of the natural products have a weak inhibitory effect on free radicals, but safety is guaranteed for human skin.

Therefore, there is a hope for a substance that has a strong active oxygen inhibitory effect and antioxidant effect and has another effect on human skin.

A representative histological change of photoaging is the accumulation of elastin, the main component of elastin fibers. Unlike normal collagen fibers, solar elastotic material is composed of macromolecules and its function is severely damaged by light.

It is said that the expression of elastin gene is remarkably promoted in skin cells exposed to ultraviolet rays, and expression of elastin gene is induced by free radicals.

Elastase is the only enzyme known so far that can break down elastin, and inhibition of it can fundamentally reduce skin aging. The main reason for the breakdown of collagen is by light.

Photoaging is initiated by signal transduction by UV rays, and skin aging can be prevented by antioxidants or retinoids and protein inhibitors that inhibit the expression of proteolytic enzymes that can block it in the early stage, and these are clinically effective is being proven

Looking at the mechanism of skin aging, first, active oxygen or free radicals are generated by ultraviolet rays or external environmental factors, and the cell membrane is attacked by the generated active oxygen or free radicals, causing inflammation and inflammation of the skin. Inflammated tissue destroys proteins or genetic materials by physiological mechanisms. Skin aging that forms wrinkles and the like occurs.

Therefore, suppressing and blocking all steps involved in the skin aging mechanism would correspond to a method that can fundamentally prevent skin aging.

Factors that cause skin inflammation include physicochemical stimuli, allergens, ultraviolet rays, oxidative stress, pathogenic infection, and secondary inflammation-related cytokines and chemokines produced by this.

The general pathway of the skin inflammatory response is an immune response to protect the human body from external stimuli, and the control of inflammation through inhibition of the inflammatory response is the main target of treatment. In the initiation and maintenance of the inflammatory response in skin inflammatory lesions, cytokines or chemokines generated during the infiltration of various inflammatory cells such as T cells or eosinophils expressing skin lymphocyte-related antigens into the lesion are involved, and related receptors are inflammatory cells , keratinocytes, fibroblasts, etc. are known to be expressed in various cells. Chemokines are a group of cytokines, and more than 50 types are currently known. They play a role in attracting appropriate immune cells to the site while the tissue undergoes an inflammatory response. When the skin is stimulated by an allergen or an irritant, the activated Langerhans cells move toward the dermis, and T cells are activated by delivering antigens to naive T cells that have not been stimulated. Memory T cells that have been exposed to an antigen once circulate along the blood vessels and migrate to the lesion site, where they are activated more rapidly, leading to an effective immune response. Representative skin diseases explained by this mechanism, such as allergic contact dermatitis, psoriasis, and atopic dermatitis, are inflammatory diseases mediated by T cells.

On the other hand, activated helper T cells stimulate B cells to cause overproduction of immunoglobulin E. Immunoglobulin E triggers inflammatory responses such as irritation, erythema, and itching, and activates cyclooxygenase (COX) signaling, which is another inflammatory pathway by stimulating mast cells. While COX-1 is intrinsically constant in tissues, COX-2 is induced by an inflammatory response and is known to be rapidly expressed within a short period of time by various stimuli. It is known that an increase in the expression of COX-2, a representative inflammatory factor, also affects the production of nitric oxide.

Currently, steroids, topical immunosuppressants, topical antibiotics, and antihistamines are used for the treatment of inflammatory skin diseases. Side effects such as purpura may occur. In particular, when steroids are applied to a wide area, the drug is systemically absorbed through the skin, which can impede growth in children and systemic side effects such as osteoporosis in adults.

Therefore, there is a need to develop a composition derived from natural substances that is safe for the human body with few side effects, imparts an antiseptic effect to the cosmetic composition, and is excellent in preventing aging, whitening and improving inflammatory skin diseases.

KR 10-2019-0012324 A

SUMMARY OF THE INVENTION It is an object of the present invention to provide a cosmetic composition comprising an Injin mugwort extract and an olive extract as active ingredients.

It is another object of the present invention to provide a cosmetic composition comprising Injin mugwort extract and olive extract as active ingredients, which exhibits antibacterial, antioxidant, whitening and anti-inflammatory effects.

Another object of the present invention is a cosmetic composition that can provide antibacterial activity against various microorganisms, prevent and improve skin aging, improve skin wrinkles, whiten effect, and improve skin inflammation, including wormwood extract and olive extract as active ingredients is to provide

In order to achieve the above object, the cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory according to an embodiment of the present invention includes Injin mugwort extract and olive extract as active ingredients.

The composition is to prevent inflammatory skin diseases selected from the group consisting of atopic dermatitis and seborrheic dermatitis.

The extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof, and after extraction, Soxhlet purification using 0.1 mol hydrochloric acid ethanol solution and 65% ethanol solution purification it has become

The composition effectively removes bacteria selected from the group consisting of Escherichia coil, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and fungi (Aspergillus brasiliensis) to provide antibacterial and antiseptic properties will have an effect.

The composition has antioxidant activity through free radical scavenging ability and MDA (malondialdehyde) scavenging ability.

The composition exhibits a whitening effect through tyrosinase inhibitory ability and melanin production inhibitory ability.

The toner according to another embodiment of the present invention is prepared by including the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition as an active ingredient.

The emulsion according to another embodiment of the present invention is prepared by including the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition as an active ingredient.

Hereinafter, the present invention will be described in more detail.

As used herein, the term "prevention" refers to preventing, inhibiting, or delaying skin aging and damage by protecting the skin, and "improvement" refers to the treatment of skin aging and damage, or improvement of symptoms even without complete healing. It means to stop the progression of damage by suppressing tremor or exacerbation and to induce some or all of the symptoms in the direction of healing.

The cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory according to an embodiment of the present invention includes an extract of ginseng mugwort and an olive extract as active ingredients.

Artemisia capillaris (Artemisia capillaris) is a herbaceous perennial plant of the Compositae family, which is native to Korea. Injin mugwort mainly uses young shoots, and is known to be effective in anti-inflammatory, antibacterial, anti-acne, antioxidant, diuretic, and jaundice. The main components include various components from chlorogenic acid and caffeic acid, which are types of polyphenols, to oleic acid and linoleic acid, which are essential oil components.

Olive is the fruit of the olive tree (olea europaea), and it is widely used in Italian and Mediterranean cuisines and plays a very important role in their food culture. Although raw olives have a characteristic bitter taste, if they are pickled in water, salt water, or alkaline solution for a certain period of time, the bitterness disappears, the inherent flavor is restored, and the texture becomes softer. Although better known as olive oil, the olives alone make an appetizer or side dish, and are also added to salads, pasta, and pizza. Although 80-85% of total calories are fat, most of them are unsaturated fatty acids that are good for the body.

In addition, olive contains vitamin E, squalene, and oleic acid, an unsaturated fatty acid, which is effective in preventing aging. It promotes blood circulation during skin massage, and the skin barrier function is strengthened due to the unsaturated fatty acids contained in olive oil, thereby increasing the skin's defense against harmful external environments.

The composition is to prevent inflammatory skin diseases selected from the group consisting of atopic dermatitis and seborrheic dermatitis.

Atopic dermatitis is an inflammatory disease accompanied by pruritus, dry skin, eczema, etc. In climate, it tends to be more severe. Therefore, the use of moisturizers to improve the dryness of the skin of atopic dermatitis and the increase in the recovery of the skin barrier function occupies an important position in the treatment of atopic dermatitis.

Because atopic skin is chronically dry, eczema is easily induced by various stimuli, it has severe itching, and has the characteristics of repeated recurrence. These pruritus and eczema are repeatedly weakened by dry skin, and if not properly managed, eventually progress to subacute and chronic lesions such as lichenification, pigment change, and erythematosus. With immunological abnormalities, superficial folliculitis, phytilosporum folliculitis, epilepsy, superficial dermatophytosis, warts, and recurrent herpes simplex are common. Bacterial infections are usually caused by coagulase-positive Staphylococcus aureus, and mainly appear as folliculitis lesions with superficial pruritus on the arms and legs. In some cases, it can cause recurrent cellulitis with systemic symptoms.

Seborrhoeic dermatitis is a chronic inflammatory skin disease that tends to occur in areas with a lot of sebum secretion (head, forehead, armpits, etc.). Erythema (red spots) and thin scales (dandruff) appear frequently. It tends to appear within the first 3 months of life and between the ages of 40 and 70, and is a very common disease, especially occurring in 3-5% of adult males.

The extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof, and after extraction, Soxhlet purification using 0.1 mol hydrochloric acid ethanol solution and 65% ethanol solution purification it has become

The composition effectively removes bacteria selected from the group consisting of Escherichia coil, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and fungi (Aspergillus brasiliensis) to provide antibacterial and antiseptic properties will have an effect.

The composition has antioxidant activity through free radical scavenging ability and MDA (malondialdehyde) scavenging ability.

The composition exhibits a whitening effect through tyrosinase inhibitory ability and melanin production inhibitory ability.

The Injin mugwort extract and the olive extract are mixed with a polar solvent such as water, ethanol, and alcohol having 1 to 6 carbon atoms, such as water, ethanol, methanol, or the like, or a mixture of alcohol and water in a mixing ratio of 1:0.1 to 1:10. It may be eluted with a solvent, and more specifically, water may be used as an extraction solvent. At this time, the extraction temperature may be 10 ℃ to 100 ℃, preferably the extract extracted at 80 ℃ to 90 ℃.

The extraction solvent may be used in an amount of 2 to 50 times, preferably 10 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for leaching in the extraction solvent, and specifically for 4 to 6 hours.

It may be a result obtained by concentrating the filtered extract under reduced pressure with a vacuum rotary concentrator, but is not limited thereto, as long as it is a cosmetic composition comprising the Injin mugwort extract and olive extract of the present invention as an active ingredient, the extract, a diluted or concentrated solution of the extract; All of the dried product obtained by drying the extract, and a crude product or purified product thereof are included.

At this time, as the dilution solvent, butylene glycol, butanediol, and propanediol are suitable.

The antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition further comprises a Lithophyllum okamurai extract.

Lithophyllum okamurai (Lithophyllum okamurai) is a red plant with a thickness of about 0.6 mm, and grows by enveloping the surface of small stones or other objects and producing lump-shaped projections. The whole body is covered with calcareous and has a pink or red color. The columnar protrusion corresponds to the branch, and the tip is round and the surface is smooth. In Korea, it is collected from Jeju and the southern coast.

In addition, the polysaccharides contained in seaweed as described above are alginic acid and fucoidan, and the alginic acid is a type of dietary fiber and activates intracellular metabolism to keep skin and hair healthy. It helps in the formation of elastic cells such as collagen and elastin in the skin. It is rich in zinc, which is called a skin mineral, which helps to get rid of skin problems such as acne and pimples.

The fucoidan (Fucoidan) is a substance secreted by seaweed to protect itself from waves and sunlight, and acts as a very excellent natural moisturizer. Through a recent study, fucoidan has been confirmed to have the effect of protecting the skin from UV rays by helping cells activate. .

The antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition is prepared by mixing the Injin mugwort extract and the olive extract with the Hokdol leaf extract. According to the synergistic effect of the mixed use, superior antibacterial, It may have antioxidant and anti-inflammatory effects.

Preferably, the antibacterial, antioxidant, whitening, and anti-inflammatory cosmetic composition may include 100 parts by weight of olive extract and 40 to 60 parts by weight of extract of Hokdol leaf based on 100 parts by weight of Injin mugwort extract. In the case of the above range, a synergistic effect of critical significance is expressed as a synergistic effect due to the interaction of each extract, and when out of the above range, the synergistic effect is rapidly reduced or almost absent.

More preferably, in the present invention, in order to promote antibacterial, antioxidant and anti-inflammatory effects and to improve palatability, a gum extract and Muruza extract may be additionally included.

The seal refers to the seeds of the prickly lotus flower, which are collected during the seed maturity period of the prickly lotus flower, and only the seeds are taken out and dried in the sun. The thorn lotus (Euryale ferox Salisb.) is an annual plant that grows in swamps or ponds and has thorns and many beard roots. The leaves that come out from the germination of seeds are small, but gradually large leaves come out and the surface is wrinkled. A flower grows from July to August with a long peduncle with thorns and hangs one at the end. Fruits are axillary, round, and seeds are wrapped in fleshy species.

The Muruza means dried seeds of cycad (Cycas revoluta Thunb.). The cycad is an evergreen shrub or small tall tree planted as a landscape tree in a park or garden, and has a height of 1 to 4 m and a diameter of 20 to 30 cm. The stem bark grows surrounded by leaf marks. The leaves are gathered at the tip of the stem, pinnately compound leaves once, and the small leaves are linear, 8 to 20 cm long, 5 to 8 cm wide, with flat edges and slightly curled backwards. Flowers bloom in the male and female trees in June to August. The male inflorescence stands upright at the end of the stem and is cylindrical, 50 to 60 cm long, 10 to 13 cm wide, and has many scale pieces. The female inflorescence is gathered and hung at the end of the main stem, and 2 to 8 ovules are alternately attached to the stem, and yellowish brown hairs are densely formed on the upper part. The fruits are coniferous, ripen in October, and the seeds are about 4cm long and red. It is planted in the southern regions of Korea and Jeju Island, and is distributed along the coast in its native areas such as Japan, China, and Taiwan. It is planted for ornamental purposes, and its fruits are edible and medicinal.

When the gum extract and Muruja extract are additionally included, the antioxidant and anti-inflammatory effects are equal to or increased compared to the case of using only the Injin mugwort extract, the olive extract, and the Hokdol leaf extract as a composite extract, The older makes it possible to provide a more palatable composition.

Preferably, the cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory of the present invention contains 100 parts by weight of olive extract, 40 to 60 parts by weight of Hokdol leaf extract, 10 to 20 parts by weight of gum extract, and 10 to 20 parts by weight of Gumin extract, based on 100 parts by weight of Injin mugwort extract. 10 to 20 parts by weight of the extract may be included. According to the above range, there is an advantage that it can be used stably at a relatively high concentration due to low cytotoxicity, and it is possible to increase the falling preference due to the peculiar odor of mugwort and seaweed.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, a solution, suspension, paste, gel, cream, lotion, powder, soap, surfactant-containing clenjeung, oil , powder foundation, emulsion foundation, wax foundation, and spray may be formulated, but are not limited thereto. More specifically, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder formulation, oil-in-water (O/W) type or water-in-oil It may be prepared as a (W/O) type formulation or ointment.

In addition, if necessary, functional substances such as whitening agents, moisturizing agents, anti-inflammatory agents, antibacterial agents, vitamins, sunscreen agents, antibiotics, anti-acne agents, perfumes, dyes may be included, and these may be included in an amount commonly used in the cosmetic field, and the cosmetic according to the present invention may be included in the composition.

The toner according to another embodiment of the present invention is prepared by including the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition as an active ingredient.

The emulsion according to another embodiment of the present invention is prepared by including the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition as an active ingredient.

The dermatologically acceptable carrier may include purified water, oil, wax, fatty acid, fatty acid alcohol, fatty acid ester, surfactant, moisture absorbent, thickener, antioxidant, viscosity stabilizer, chelating agent, buffer, preservative, lower alcohol, etc. However, the present invention is not limited thereto, and the types and concentrations thereof vary, and include parts that those skilled in the art can change within the scope of the present invention.

Cosmetics prepared from the cosmetic composition of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. Cosmetics with emulsified formulations include nutritional lotions, creams, and essences, and solubilized cosmetics include softened lotions.

The cosmetic may be prepared in the form of an adjuvant that can be applied topically or systemically commonly used in the art by containing a dermatologically acceptable medium or base in addition to the extract of the present invention.

Suitable cosmetic formulations include, for example, solutions, gels, solid or kneaded dry products, emulsions obtained by dispersing an oily phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), non-ionic It may be provided in the form of a vesicular dispersant, cream, skin, lotion, powder, ointment, spray or in the form of a cone stick. In addition, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nutrient lotion, various creams, essence, pack, foundation, etc. and cleansing, face wash, soap, treatment or liquid cosmetics etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, essence, nourishing essence, pack, soap, shampoo, cleansing foam. , cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, or eye shadow formulations.

According to the cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory comprising the extract of Injin mugwort extract and olive extract of the present invention as active ingredients, it prevents skin aging, skin wrinkles, whitening effect and skin inflammation through antioxidant, whitening and anti-inflammatory activities. And it can be improved, and it is possible to present excellent preservative power to cosmetics or raw materials contained therein through antibacterial activity.

1 is an experimental result of DPPH free radical scavenging activity when the antibacterial, antioxidant, whitening and anti-inflammatory composition according to an embodiment of the present invention is treated by concentration.
2 is an experimental result of MDA (malondialdehyde) scavenging ability when the antibacterial, antioxidant, whitening and anti-inflammatory composition according to an embodiment of the present invention is treated by concentration.
3 is an experimental result for tyrosinase scavenging ability when the antibacterial, antioxidant, whitening and anti-inflammatory composition according to an embodiment of the present invention is treated by concentration.
4 is an experimental result for the amount of melanin produced when the antibacterial, antioxidant, whitening and anti-inflammatory composition according to an embodiment of the present invention is treated by concentration.
5 is an experimental result on the scavenging ability of nitric oxide and prostaglandins of the composition for antibacterial, antioxidant, whitening and anti-inflammatory according to an embodiment of the present invention.
6 is a graph comparing the amount of inflammatory cytokines when the antibacterial, antioxidant, whitening and anti-inflammatory composition according to an embodiment of the present invention is treated by concentration.
7 is a graph comparing the amount of interleukin-6 when the antibacterial, antioxidant, whitening and anti-inflammatory composition according to an embodiment of the present invention is treated by concentration.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of extract]

One. Preparation of Injin Mugwort Extract

After washing and drying Injin mugwort, it was pulverized. The pulverized product was mixed with water, maintained at 80 to 90° C. for 6 hours, cooled, filtered with Whatman filter paper, and the filtrate was concentrated under reduced pressure at 70° C. to obtain a primary Injin mugwort extract powder. This Injin mugwort extract powder was put in a Soxhlet apparatus and refluxed with 0.1 mol aqueous hydrochloric acid ethanol solution, and then the first purified Injin mugwort extract powder was again put in a 65% ethanol aqueous solution heated to 50° C. The filtrate was cooled to 10° C. or less, and the recrystallized material was vacuum-dried at 60° C. to prepare a final Injin mugwort extract (AE).

2. Preparation of Olive Extract

An olive extract (OE) was prepared in the same manner as the Injin mugwort extract (AE).

3. Preparation of a mixture of wormwood extract and olive extract

Injin mugwort extract (AE) and olive extract (OE) prepared above were mixed with Injin mugwort extract (AE), olive extract (OE), and propanediol (Propanediol) at a ratio of 0.9/0.1/9 to effectively use Injin mugwort extract and olive extract A cosmetic composition (MX1) for antibacterial, antioxidant, whitening and anti-inflammatory including as a component was prepared.

[Experimental Example 1: Experiment on the effect of inhibiting the growth of microorganisms by the antibacterial activity of the composition]

In order to measure the antibacterial activity of a cosmetic composition (MX1) for antibacterial, antioxidant, whitening and anti-inflammatory containing ginseng mugwort extract and olive extract as active ingredients, 3 types of bacteria and 2 types of fungi, which are mainly evaluated in the cosmetic industry, are listed in Table 1 and They were selected together, and strains causing acne, athlete's foot, and dandruff were selected as shown in Table 2, and strains causative of urology and sexually related diseases as well as bacterial bacteria on the skin were selected as shown in Table 3 and tested.

strain name strain number Germ Escherichia coil ATCC 8739 Staphylococcus aureus ATCC 6538 Pseudomonas aeruginosa ATCC 9027 fungus Candida albicans ATCC 10231 Aspergillus brasillensis ATCC 16404

strain name strain number Propionibacterium acnes ATCC 6919 Malassezia furfur ATCC 14521 Trichophyton mentagrophytes ATCC 9533

strain name strain number Gardnerella vaginalis ATCC 14019 Neisseria gonorrhoeae ATCC 19424 Chlamydia trachomatis ATCC VR-885 Ureaplasma urealyticum ATCC 27618 Ureaplasma parvum ATCC 27815 Mycoplasma genitalium ATCC 33530 Mycoplasma hominis ATCC 23114 Trichomonas vaginalis ATCC 30001 Treponema phagedenis ATCC 27807 Mycobacterium smegmatis ATCC 19420

In order to measure the antimicrobial activity using the selected strain, the MIC test was performed.

MIC is an abbreviation of minimal inhibitory concentration and means the minimum concentration of an antimicrobial agent that can inhibit the growth of bacteria.

For the test, prepare 2×10 ⁷ CFU/mL for bacteria and 2×10 ⁶ CFU/mL for fungi. Put the sample to be tested in the microtube separately for bacteria and fungi, and mix well with NB (nutrient broth) for bacteria and PDB (potato dextrose broth) for fungi. Add 0.1 mL of each prepared sample to the top compartment of the 96-well plate (separately insert for bacteria and fungi).

After filling the entire 96-well plate with 0.1 mL of the strain prepared above, dilute by 1/2 from the top column. At this time, the lower compartment is used as a blank by placing only the bacterial solution without dilution with the sample. When all steps are completed, the 96-well plate is incubated at 37°C. The evaluation of the results is as follows. Three types of bacteria are checked after 18 to 24 hours, for C. albicans , after 32 to 56 hours, and for A. brasillensis , after 36 to 72 hours, and visually measure the turbidity to confirm the growth of the bacteria. At this time, the minimum inhibitory concentration is indicated by setting the concentration at the time when the turbidity disappears.

The results are shown in Tables 4 to 6 below. According to Table 4, the present composition showed a minimum inhibitory concentration at a concentration of 0.5% to 1% for 5 types of test strains, thereby confirming that it exhibits a uniform antimicrobial activity regardless of the type of bacteria.

No. Sample E. coli S. aureus P. aeruginosa C. albicans A. brasillensis One 1,2-HD 1.0 2.0 1.0 1.0 1.0 2 MX1 0.5 1.0 1.0 1.0 1.0

(unit: %)

No. Sample P.acnes M.furfur T. Mentagrophytes One 1,2-HD 2.0 2.0 2.0 2 MX1 1.0 1.0 1.0

(unit: %)

No. strain MIC value One Gardnerella vaginalis 1.0 2 Neisseria gonorrhoeae 0.5 3 Chlamydia trachomatis 1.0 4 Ureaplasma urealyticum 1.0 5 Ureaplasma parvum 1.0 6 Mycoplasma genitalium 10.0 7 Mycoplasma hominis 1.0 8 Trichomonas vaginalis 0.5 9 Treponema phagedenis 1.0 10 Mycobacterium smegmatis 0.5

(unit: %)

According to Table 5, the composition showed the minimum inhibitory concentration at a concentration of 1% for each strain causing acne, athlete's foot, and dandruff. By showing a minimum inhibitory concentration of 0.5% to 1.0% for

The above results were evaluated in comparison with 1,2-hexanediol, which is most widely used as a preservative in the cosmetic industry, and the same level of antibacterial activity was confirmed at about half the concentration of 1,2-hexanediol for most of the test strains.

Therefore, it can be seen that the cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory containing the extract of Injin mugwort extract and olive extract as active ingredients exhibits excellent antibacterial activity in a wide range.

[Experimental Example 2: Test of antiseptic effect of cosmetic formulations containing compositions]

In order to measure the preservative power of two basic formulations including antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition (MX1) containing ginseng mugwort extract and olive extract as active ingredients, three types of bacteria evaluated by the cosmetic industry and Two types of fungi were selected as shown in Table 1, and a preservative force test was performed. The two basic formulations prepared to proceed with the preservative test are the same as Formulation Example 1 and Formulation Example 2 below.

Formulation Example 1. Toner

The toner was prepared in a conventional manner according to the composition components and composition ratios in Table 7 below.

Raw material content(%) A Disodium EDTA 0.02 citric acid 0.03 sodium citrate 0.10 Glycerin 2.00 Water To 100 B PEG-60 Hydrogenated Casor Oil 0.10 Perfume 0.02 1,3-Butylene Glycol 2.00 C MX1 1.00

Formulation Example 2. Emulsion

The emulsion was prepared in a conventional manner according to the composition components and composition ratios in Table 8 below.

Raw material content(%) A Ammonium Acryloyldimethyltaurate/VP Copolymer 1.50 Caprylic/Capric Triglycerides 10.00 B Cetearyl Olivate/Sorbitan Olivate 5.00 Water To 100 C MX1 1.00

In order to proceed with the antiseptic test using the selected strain, prepare the strain to be tested at 2×10 ⁸ CFU/mL for bacteria and 2×10 ⁷ CFU/mL for fungi. The sample for evaluating the preservative power is divided into sterilized containers by exactly 20.0 g. At this time, prepare as many as the number of test target bacteria for each sample. Inoculate 0.2 mL each in a container containing 20.0 g of the inoculated bacteria prepared above, and mix well so that the inoculated bacteria solution is uniformly distributed in the sample. After inoculation, incubate at 25°C for 4 weeks, collect 1 g of the sample at intervals of 1 week, mix it with 9 mL of diluent, and dilute step-by-step to measure the number of viable cells. The reduction in bacteria at each bacteria recovery time is calculated according to the formula below.

Decrease rate: D ₀ -D _X /D ₀ Х100(%)

Log reduction: Log(D ₀ )-Log(D _X )

D ₀ : Number of inoculated bacteria (CFU/g)

D _X : Number of bacteria recovered at Week X (CFU/g)

The judging criteria are as follows.

[Criteria]

Bacteria: Reduced by 99.9% or more in 1 week (Log reduction 3.0 or more), no growth during the test period thereafter

Fungi: Reduced by more than 90% (Log reduction 1.0 or more) at the 1st week, no growth during the test period thereafter

The results are shown in Tables 9 and 10 below.

0 d 7 d 14 d 21 d 28 d Bacteria 2.36 x 10 ⁶ 99.99% 99.99% 99.99% 99.99% Yeast 1.50 x 10 ⁵ 99.99% 99.99% 99.99% 99.99% Fungi 1.93 x 10 ⁵ 99.98% 99.99% 99.99% 99.99%

0 d 7 d 14 d 21 d 28 d Bacteria 2.36 x 10 ⁶ 99.99% 99.99% 99.99% 99.99% Yeast 1.50 x 10 ⁵ 99.99% 99.99% 99.99% 99.99% Fungi 1.93 x 10 ⁵ 99.99% 99.99% 99.99% 99.99%

According to Tables 9 and 10, the toner and emulsion containing the present composition showed a reduction rate that met the pass criteria for the first week for 5 types of test strains, and thereafter, by confirming that there was no growth until the fourth week, the form of the formulation or It can be seen that it shows even antiseptic power regardless of the type of bacteria.

[Experimental Example 3: Antioxidant effect test of composition]

In order to confirm the antioxidant activity of the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition (MX1) containing ginseng mugwort extract and olive extract as active ingredients, free radical scavenging activity and MDA (malondialdehyde) reduction activity were evaluated.

1. Evaluation of DPPH free radical scavenging ability

10 mg DPPH was dissolved in 100 mL methanol (MeOH) to make 100 μg/mL. This composition was prepared by dissolving 200 mg in 10 mL of MeOH, and was prepared by dilution in stages, and ascorbic acid, which is well known for its excellent free radical scavenging ability, was set as a positive control.

1 mL of DPPH was added to each solution and the solution was kept dark and allowed to stand for exactly 30 minutes. The UV absorption of each solution was recorded at 517 nm. The reaction was allowed to proceed at 37 °C for 30 min and the absorbance was monitored at 517 nm with a microplate reader. The % radical scavenging activity (% RSA) was determined compared to the DMSO containing control. The scavenging of free radicals by DPPH as a radical scavenging activity (%) was calculated as follows, and the results are shown in FIG. 1 below.

% RSA = (Control Absorbance - Absorbance) X 100 / Control Absorbance

According to FIG. 1, when the composition is used from 0.05% to 2%, that is, it was confirmed that the DPPH free radical scavenging ability is improved as the content is increased.

According to the experimental results, in the case of the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition (MX1) containing Injin mugwort extract and olive extract as active ingredients, DPPH radical scavenging ability is excellent, so it can be said that the anti-aging effect by antioxidant is excellent. can

2. MDA (malondialdehyde) scavenging ability evaluation

MDA is the final product of linoleic acid and occurs in proportion to the level of lipid peroxidation. Unsaturated fatty acids are oxidized in a certain direction by an enzymatic reaction in a living body to become water and carbon dioxide gas. On the other hand, in the case of non-enzymatic autoxidation, a substance in which molecular oxygen is directly bonded to the double bond of an unsaturated fatty acid is produced. This is called lipid peroxide. This material is unstable and highly reactive because it is a state in which unpaired electrons are created in part of a molecule (free radicals). Therefore, the lipid peroxide generated in the living body has the side effect of causing damage to the biological membrane system, inactivation or denaturation of enzymes, proteins, hormones, vitamins, etc., causing cell function deterioration or necrosis. Clinically, the relationship between aging, arteriosclerosis, thrombosis, diabetes, skin disease, respiratory disease, liver disease, etc. has been reported. It is known that when reactive oxygen species are generated by an inflammatory reaction, lipid peroxidation proceeds and MAA protein is secreted, which worsens cytokine and immune responses.

After preparing the composition by diluting it step-by-step with purified water, 4 mL of the sample and 1 mL of 13% linoleic acid were mixed using a water bath stirrer. Thereafter, 2.5 mL of thiobarbituric acid (TBA) solution was added, reacted for 10 minutes, and centrifuged at 25° C. at 5000 g for 10 minutes to separate the supernatant, and the absorbance was measured at 532 nm using a microplate reader. .

The MDA concentration was calculated by the following formula, and the results are shown in FIG. 2 below.

MDA contents (%) = 100 [(A _c - A _s )/A _c ]

A _c : control absorbance

A _s : absorbance of sample group

According to FIG. 2, it was confirmed that when the composition was used from 0.01% to 2%, that is, the MDA production inhibitory ability increased as the content increased.

Therefore, the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition (MX1) containing ginseng mugwort extract and olive extract as active ingredients reduces lipid peroxidation caused by free radicals in the body and protects cells, thereby inhibiting aging by antioxidants. It can be said that the action is excellent.

[Experimental Example 4: Test for whitening effect of the composition]

In order to confirm the whitening effect of the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition (MX1) containing Injin mugwort extract and olive extract as active ingredients, tyrosinase inhibitory ability and melanin production inhibitory ability were evaluated.

1. Inhibition of tyrosinase production

Tyrosinase is an enzyme involved in melanin biosynthesis, and in this experiment, the activity inhibiting ability of tyrosinase was evaluated using the maximum absorption wavelength of 490 nm of DOPA quinone produced by the action of substrate tyrosine and tyrosinase. Mushroom tyrosinase and L-tyrosine used in the experiment were purchased commercially from Sigma-Aldrich in the US and measured.

Prior to the experiment, Kojic acid as a positive control and the composition were prepared by diluting to an appropriate concentration using 0.05M sodium phosphate buffer (pH 6.5). After putting 220 μl of 0.1 M sodium phosphate buffer (pH 6.5), 20 μl of sample, and 20 μl of 2,000 U/mL mushroom tyroinase into each well of a 96-well plate, pre-culture was performed at 37° C. for 5 minutes.

Then, 40 μl of 1.5 mM L-tyrosine was added, and after reacting at 37° C. for 10 minutes, absorbance was measured at 490 nm using a microplate reader.

The inhibition rate of tyrosinase was calculated by the following formula, and the results are shown in FIG. 3 below.

Tyrosinase inhibition (%) = {1- (standard / absorbance of sample ÷ absorbance of blank)} × 100

According to FIG. 3, it was confirmed that when the composition was used from 0.01% to 2%, that is, the tyrosinase activity inhibitory ability increased as the content increased. In addition, it was confirmed that the inhibition rate of tyrosinase activity at a concentration of 1% of the composition was at a similar level when compared with 100 μg/mL of Kojic acid, a positive control.

Therefore, it can be seen that the antibacterial, antioxidant, whitening, and anti-inflammatory cosmetic composition (MX1) containing the extract of ginseng mugwort and olive extract as active ingredients inhibits melanin production through tyrosinase inhibitory ability, thereby having an excellent effect on skin whitening.

2. Inhibition of melanogenesis by B16F10 melanocytes

B16F10 melanin-forming cells are a cell line derived from a mouse, and are cells that secrete a black pigment called melanin. B16F10 melanin-forming cells used in this test example were pre-owned from ATCC (American Type Culture Collection) and used.

B16F10 melanin-forming cells were aliquoted in a 6-well plate at a concentration of 2×10 ⁶ cells/mL per well, and the cells were attached thereto and then cultured by adding 0.01% to 0.1% of the composition in an incubator at 35° C., respectively. After culturing for 72 hours, the cells were removed with trypsin-EDTA (ethylenediaminetetraacetic acid), the number of cells was counted, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out by modifying Lotan's method. After washing the cell pellet once with PBS (phosphate buffer saline), 1 mL of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) is added and vortexed for 5 minutes to disrupt the cells. did. To the cell filtrate obtained by centrifugation, 1N NaOH was added to dissolve the extracted melanin, the absorbance of melanin was measured at 405 nm with a microplate reader, and then melanin was quantified to measure the melanin production inhibition rate (%) of the sample. For a comparative experiment, arbutin, a substance known to have an inhibitory effect on melanogenesis, was used as a positive control.

The inhibition rate (%) of melanin production of B16F10 melanin-forming cells was calculated by the following formula, and the results are shown in FIG. 4 below.

Melanogenesis inhibition rate (%) = {(A-B)/A} X 100

According to Figure 4, when the composition is used from 0.01% to 0.1%, that is, it was confirmed that the higher the content, the higher the melanin production inhibitory ability.

Therefore, it can be seen that the antibacterial, antioxidant, whitening, and anti-inflammatory cosmetic composition (MX1) containing the extract of ginseng mugwort and olive extract as active ingredients inhibits melanin production through tyrosinase inhibitory ability, thereby having an excellent effect on skin whitening.

[Experimental Example 5: Antioxidative and anti-inflammatory effect test of composition]

The anti-inflammatory effect was evaluated to confirm the anti-inflammatory, anti-oxidative, whitening and anti-inflammatory cosmetic composition (MX1) containing ginseng mugwort extract and olive extract as active ingredients (MX1) to suppress and improve skin inflammation.

1. LPS (Lipopolysaccharide)-induced Nitric oxide and PGE ₂ production inhibitory ability

To check the anti-inflammatory effect of the composition, macrophages were used to examine the effect on the production of nitric oxide (NO) and prostaglandin E ₂ (PGE ₂ ).

Macrophages dissolve foreign substances by releasing active oxygen from the cell membrane in order to phagocytose bacteria or fungi that invaded from the outside. However, as the activity of macrophages continuously increases, this reaction becomes frequent and the amount of secretion of active oxygen increases, the active oxygen leaks out to the outside of the cell and attacks other blood vessel walls or tissues, resulting in disease. In addition, prostaglandin E ₂ is also an inflammatory factor generated by the activity of macrophages, and causes symptoms such as vasodilation, bronchodilation, gastric acid suppression, inflammation, and fever. The anti-inflammatory efficacy of any substance can be measured by using the characteristics of these macrophages. The cells used in the experiment were RAW264.7 (Mouse macrophage), 37°C, 5% CO ₂ , 10% Fetal bovine serum (FBS, Lonza), and 50 μg/mL of streptomycin (Sigma) added to Dulbecco's modified It was cultured in Eagle's medium (DMEM, Invitrogen). The cells were put into a 96-well plate at a concentration of 1×10 ⁵ cells/well, and then cultured at 37° C., 5% CO ₂ in an incubator for 24 hours. The culture medium was removed and the sample was replaced with a new medium, followed by pre-culture at 37° C., 5% CO ₂ in an incubator for 30 minutes. After incubation for 30 minutes, LPS was treated to be 1 μg/mL and cultured at 37° C., 5% CO ₂ in an incubator for 24 hours. After incubation, 100 μl of the supernatant is taken and transferred to a new 96-well plate. After mixing griess reagent A and B 1:1, 100 μl is dispensed. Immediately after treatment with Griess reagent, absorbance is measured at 540 nm using a microplate reader. The NO production rate (%) of macrophages was calculated by the following formula, and the results are shown in FIG. 5 below.

NO production (%) = absorbance of sample addition / absorbance of control × 100

The production of PGE ₂ was also performed in a similar manner to the above method. After culturing, 100 μl of the supernatant was taken and transferred to a new 96-well plate, and then diluted 10 to 20 times using a culture medium in consideration of the experimenter's tendency and the experimental environment. Put 100 μl of culture medium into the NSB well of the Anti-Mouse IgG plate, 100 μl of standard and sample (diluted supernatant) into each well, and 50 μl of blue PGE _2- AP conjugate into all wells except for the blank well. put each Put 50 μl of culture medium into the NSB well, 50 μl of yellow PGE ₂ antibody into all wells except the blank and NSB well, and use an orbital shaker at room temperature (22-25° C.) for 2 hours at 500 rpm. was shaken. After 2 hours, the contents of the plate were discarded, washed 3 times with 400 μl of washing buffer, 200 μl of substrate solution was added to each well, and incubated at room temperature (22-25° C.) for 45 minutes. After incubation, 50 μl of stop solution was added to each well, and absorbance was immediately measured at 405 nm using a microplate reader. The PGE ₂ production rate (%) of macrophages was calculated by the following formula, and the results are shown in FIG. 5 below.

Standard curve = using the natural logarithm, we get the equation of y=A*ln(x) + B.

% bound = [(absorbance of sample-NSB absorbance)/(Blank absorbance-NSB absorbance)]*100

Using the above two equations, PGE ₂ production amount = dilution factor *(e(%bound-B))/A

As shown in Figure 5, when the composition is used from 0.01% to 0.1%, that is, as the content increases, it was confirmed that NO and PGE ₂ production is reduced.

Therefore, it can be seen that the composition has an excellent anti-inflammatory effect against inflammatory reactions such as fever, edema, and erythema caused by external stimuli such as antigen.

2. Inhibitory ability of inflammatory cytokines induced by UV irradiation

In order to confirm the anti-inflammatory effect of the composition, the effect on the production of TNF-α, COX-2, and IL-1b, which are one of the initial inflammatory molecules, was investigated. The formation of pro-inflammatory cytokines such as TNF-α is a process in which arachidonic acid is converted to prostaglandin and nitric oxide (NO) formation due to the activity of phospholipase A2. lead to the process. The cells used in the experiment were HaCaT (Human Skin Keratinocytes cell line), 37°C, 5% CO ₂ , 10% Fetal bovine serum (FBS, Lonza), and Dulbecco's modified with 50 μg/mL streptomycin (Sigma) added. It was cultured in Eagle's medium (DMEM, Invitrogen). After incubation, the cells were stressed by UV irradiation for 1 hour. As a control, cells irradiated with UV without processing the sample were used.

For RNA analysis, total RNA in cells was extracted from cell culture using Trizol reagent (Invitrogen, USA). The purity and integrity of RNA was confirmed by measuring the A260nm/A280nm ratio, and the RNA yield was measured by absorbance at 260nm.

For cDNA synthesis, 3 μg of total RNA was added with Oligo dT 15 (500 ng/μl) primer, dNTP (10 mM), RTase inhibitor (40 U/μl), and Powerscript Ⅱ RTase (Clontech, USA) at 25°C for 10 Minute primer annealing, cDNA synthesis at 42°C for 60 minutes, and RTase denaturation at 95°C for 5 minutes. For PCR, 3 µl of cDNA, 5 µl of 10X taq polymerase buffer, 2 µl of 10 mM dNTP, 2 µl of 10 pmol primer, and 0.5 µl of taq polymerase were each added to amplify GAPDH of TNF-α, COX-2, and IL-1b from cDNA. After mixing and adding distilled water, the volume was adjusted to 50 μl and amplified. The fryer sequence is shown in Table 11.

The product generated by PCR was electrophoresed on 1% agarose gel and confirmed with an image analyzer (UGEN, U:Genius), and the density of each band was measured using a density measuring program (Gene Tools from Syngene). measured.

Gene Primer GAPDH F: 5'- AAC GAA TTT GGT CGA ACA GC-3' R: 5'-TGA GGA GGG ATT CAG TG-3' IL-1b F: 5' - GTC TCT GAA TCA GAA ATC CTT CTA TC - 3' R: 5' - CAT GTC AAA TTT CAC TGC TTC ATC C - 3' TNF-a F: 5' - CAA AGT AGA CCT GCC CAG AC - 3' R: 5' - GAC CTC TCT CTA ATC AGC CC - 3' COX-2 F: 5' - GTC CCT GAG CAT CTA CGG TTT G - 3' R: 5' - CCC ATT CAG GAT GCT CCT GTT - 3'

As shown in FIG. 6 , when the composition is used from 0.01% to 0.1%, that is, as the content increases, the inhibition of inflammatory cytokine expression is superior, indicating that it has a high anti-inflammatory effect.

Therefore, it can be seen that the composition has an excellent anti-inflammatory effect on inflammatory reactions such as fever, edema, and erythema by reducing skin irritation caused by various external stimuli (UV rays, antigens, etc.).

3. Interleukin-6 inhibitory ability induced by UV irradiation

In order to confirm the anti-inflammatory effect of the composition, the effect on the production of interleukin-6 (IL-6), which is one of the inflammatory molecules, was investigated. Interleukin-6 is a protein that mediates fever and acute inflammatory reactions, and is known to cause various skin diseases such as atopy and psoriasis. The cells used in the experiment were HaCaT (Human Skin Keratinocytes cell line), 37°C, 5% CO ₂ , 10% Fetal bovine serum (FBS, Lonza), and Dulbecco's modified with 50 μg/mL streptomycin (Sigma) added. It was cultured in Eagle's medium (DMEM, Invitrogen). The cells were put into a 96-well plate at a concentration of 1×10 ⁵ cells/well, and then cultured at 37° C., 5% CO ₂ in an incubator for 24 hours. The culture medium was removed and the sample was replaced with a new medium, followed by pre-culture at 37° C., 5% CO ₂ in an incubator for 30 minutes. After incubation, the cells were stressed by UV irradiation for 1 hour. As a control, cells irradiated with UV without processing the sample were used. After incubation, 100 μl of the supernatant was taken and transferred to a new 96-well plate, and the experiment was performed using the IL-6 Human ELISA Kit from Thermo Fisher.

As shown in FIG. 7 , when the composition was used from 0.01% to 0.1%, that is, as the content increased, the inhibition of interleukin-6 expression was superior, indicating that it had a high anti-inflammatory effect.

Therefore, by reducing skin irritation caused by various external stimuli (ultraviolet rays, antigens, etc.), it is known that the composition has an excellent anti-inflammatory effect against not only general inflammatory reactions such as fever, edema, and erythema, but also acute inflammatory reactions such as atopy. can

[Preparation Example 2: Preparation of complex extract]

One. Preparation of complex extracts

Hokdol leaf extract (LE), gum extract (FE) and Muruza extract (GE) were prepared in the same manner as the Injin mugwort extract (AE) prepared in Preparation Example 1.

2. Cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory

In the same manner as the antibacterial, antioxidant, whitening, and anti-inflammatory cosmetic composition (MX1) containing the extract (AE) and olive extract (OE) prepared in Preparation Example 1, the extract (AE) and olive extract (OE), Hokdol leaf extract (LE), gum extract (FE) and Muruza extract (GE) were mixed in the content range shown in Table 12 below to prepare antibacterial, antioxidant, whitening and anti-inflammatory cosmetic compositions.

MC1 MC2 MC3 MC4 MC5 MC6 AE 100 100 100 100 100 100 OE 100 100 100 100 100 100 LE - 30 40 50 60 70 FE - - 5 10 20 30 GE - - 5 10 20 30

(Unit: parts by weight)

[Experimental Example 6: Antibacterial, antioxidant, whitening and anti-inflammatory effect test of complex extract]

After performing the same experiment as that of MX1 conducted in Experimental Examples 1 to 5, the effect tests of the antibacterial, antioxidant, whitening and anti-inflammatory compositions (MC1 to MC6) according to Preparation Example 2 were conducted.

For the relative comparison of the results, the effect of MC1 mixed with Injin mugwort extract and olive extract was set to an index of 5, and the composite extracts MC2 to MC6 were comparatively evaluated as an index, and the results are shown in Table 13 below.

The higher the index, the better the effect.

MC1 MC2 MC3 MC4 MC5 MC6 antibacterial activity 5 6 7 8 9 7 antioxidant activity 5 5 6 7 8 6 anti-inflammatory effect 5 6 6 7 7 6

(Unit: Index)

As shown in Table 3, it was confirmed that the MC1 and the complex extracts MC2 to MC6, which were mixed with the Injin mugwort extract and the olive extract, showed an effect equal to or superior to that of MC1, and the complex extract (MC3 to MC6) was stable due to low cytotoxicity. As it increased, it showed excellent antibacterial, antioxidant, whitening and anti-inflammatory effects.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (8)

Contains Injin mugwort extract and olive extract as active ingredients.
A cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory. The method of claim 1,
The composition is to prevent inflammatory skin diseases selected from the group consisting of atopic dermatitis and seborrheic dermatitis
A cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory. The method of claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 6 carbon atoms, and mixtures thereof,
After extraction, Soxhlet purification using 0.1 mol hydrochloric acid ethanol solution and 65% ethanol aqueous solution
A cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory. The method of claim 1,
The composition effectively removes bacteria selected from the group consisting of Escherichia coil, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and fungi (Aspergillus brasiliensis) to provide antibacterial and antiseptic properties that has an effect
A cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory. The method of claim 1,
The composition has antioxidant activity through free radical scavenging ability and MDA (malondialdehyde) scavenging ability
A cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory. The method of claim 1,
The composition exhibits a whitening effect through tyrosinase inhibitory ability and melanin production inhibitory ability
A cosmetic composition for antibacterial, antioxidant, whitening and anti-inflammatory. Claims 1 to 6, comprising the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition according to any one of claims
toner. Claims 1 to 6, comprising the antibacterial, antioxidant, whitening and anti-inflammatory cosmetic composition according to any one of claims
emulsion.

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